Restriction map for
pF1KA1429
(Circular) (Six-base) MAPSORT of: pF1KA1429.seq Check: 2856 from: 1 to: 8504
pF1KA1429 8504 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 920 1403 3760 5004 5049 5542 920
Size: 483 2357 1244 45 493 3882
Fragments arranged by size:
3882 2357 1244 493 483 45
Acc65I G'GTAC_C
Cuts at: 5520 5520
Size: 8504
AclI AA'CG_TT
Cuts at: 8344 8344
Size: 8504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1109 1143 3026 3194 3446 3722 4037 4615
Size: 24 34 1883 168 252 276 315 578
Cuts at: 4615 4742 6329 6761 7114 7776 1085
Size: 127 1587 432 353 662 1813
Fragments arranged by size:
1883 1813 1587 662 578 432 353 315
276 252 168 127 34 24
AfeI AGC'GCT
Cuts at: 165 1439 3215 4819 165
Size: 1274 1776 1604 3850
Fragments arranged by size:
3850 1776 1604 1274
AflII C'TTAA_G
Cuts at: 1603 4546 1603
Size: 2943 5561
AflIII A'CryG_T
Cuts at: 2910 3017 4144 5496 5856 7656 7937 2910
Size: 107 1127 1352 360 1800 281 3477
Fragments arranged by size:
3477 1800 1352 1127 360 281 107
AleI CACnn'nnGTG
Cuts at: 2201 2201
Size: 8504
AlwNI CAG_nnn'CTG
Cuts at: 718 1882 4557 4638 5809 7247 718
Size: 1164 2675 81 1171 1438 1975
Fragments arranged by size:
2675 1975 1438 1171 1164 81
ApaLI G'TGCA_C
Cuts at: 7342 7342
Size: 8504
ApoI r'AATT_y
Cuts at: 783 1007 1230 1325 1425 2292 3284 4303 4316
Size: 224 223 95 100 867 992 1019 13
Cuts at: 4316 4458 5508 7857 8113 783
Size: 142 1050 2349 256 1174
Fragments arranged by size:
2349 1174 1050 1019 992 867 256 224
223 142 100 95 13
AseI AT'TA_AT
Cuts at: 20 3845 4406 20
Size: 3825 561 4118
Fragments arranged by size:
4118 3825 561
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8504
AvaI C'yCGr_G
Cuts at: 159 5194 5296 5524 159
Size: 5035 102 228 3139
Fragments arranged by size:
5035 3139 228 102
BamHI G'GATC_C
Cuts at: 3178 5529 3178
Size: 2351 6153
BanI G'GyrC_C
Cuts at: 5520 5934 6182 6217 5520
Size: 414 248 35 7807
Fragments arranged by size:
7807 414 248 35
BanII G_rGCy'C
Cuts at: 255 767 1521 2310 4383 4953 5518 6548 255
Size: 512 754 789 2073 570 565 1030 2211
Fragments arranged by size:
2211 2073 1030 789 754 570 565 512
BbeI G_GCGC'C
Cuts at: 5938 6186 5938
Size: 248 8256
BbsI GAAGACnn'nnnn_
Cuts at: 1097 1840 2251 2681 3728 1097
Size: 743 411 430 1047 5873
Fragments arranged by size:
5873 1047 743 430 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1861 1895 1861
Size: 34 8470
BciVI GTATCCnnnnn_n'
Cuts at: 1070 6402 7458 8504 1070
Size: 5332 1056 1046 1070
Fragments arranged by size:
5332 1070 1056 1046
BclI T'GATC_A
Cuts at: 1553 1584 1866 2325 3981 4683 6024 1553
Size: 31 282 459 1656 702 1341 4033
Fragments arranged by size:
4033 1656 1341 702 459 282 31
BfrBI ATG'CAT
Cuts at: 2374 2663 5064 2374
Size: 289 2401 5814
Fragments arranged by size:
5814 2401 289
BglI GCCn_nnn'nGGC
Cuts at: 3069 3069
Size: 8504
BglII A'GATC_T
Cuts at: 2478 2964 4737 6019 8056 2478
Size: 486 1773 1282 2037 2926
Fragments arranged by size:
2926 2037 1773 1282 486
BlpI GC'TnA_GC
Cuts at: 167 5616 167
Size: 5449 3055
Bme1580I G_kGCm'C
Cuts at: 351 6129 6222 7346 351
Size: 5778 93 1124 1509
Fragments arranged by size:
5778 1509 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 5716 5797 6132 8276 5716
Size: 81 335 2144 5944
Fragments arranged by size:
5944 2144 335 81
BmtI G_CTAG'C
Cuts at: 4111 4111
Size: 8504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4062 6871 4062
Size: 2809 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 2078 2581 3938 4057 4579 4604 4829 5153
Size: 1431 503 1357 119 522 25 225 324
Cuts at: 5153 647
Size: 3998
Fragments arranged by size:
3998 1431 1357 522 503 324 225 119
25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 1909 5093 5354 5680 6559 7273 7571 1505
Size: 404 3184 261 326 879 714 298 2438
Fragments arranged by size:
3184 2438 879 714 404 326 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 2350 3917 4366 4891 8208 2350
Size: 1567 449 525 3317 2646
Fragments arranged by size:
3317 2646 1567 525 449
BsaAI yAC'GTr
Cuts at: 1590 2911 5857 1590
Size: 1321 2946 4237
Fragments arranged by size:
4237 2946 1321
BsaBI GATnn'nnATC
Cuts at: 6 1940 4511 4736 5435 6018 8055 6
Size: 1934 2571 225 699 583 2037 455
Fragments arranged by size:
2571 2037 1934 699 583 455 225
BsaHI Gr'CG_yC
Cuts at: 5935 6183 6885 8381 5935
Size: 248 702 1496 6058
Fragments arranged by size:
6058 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5693 6214 6892 7303 7450 7724 5693
Size: 521 678 411 147 274 6473
Fragments arranged by size:
6473 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2076 2106 3218 3248 3434 3464 3911 3941 2076
Size: 30 1112 30 186 30 447 30 6639
Fragments arranged by size:
6639 1112 447 186 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 650 731 949 2350 5182 650
Size: 81 218 1401 2832 3972
Fragments arranged by size:
3972 2832 1401 218 81
BseYI C'CCAG_C
Cuts at: 554 1480 5929 6687 7352 7760 554
Size: 926 4449 758 665 408 1298
Fragments arranged by size:
4449 1298 926 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2479 3114 3665 2479
Size: 635 551 7318
Fragments arranged by size:
7318 635 551
BsiEI CG_ry'CG
Cuts at: 104 6046 6092 7322 104
Size: 5942 46 1230 1286
Fragments arranged by size:
5942 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2196 2310 3244 4230 4383 4953 5183 5518 6296
Size: 114 934 986 153 570 230 335 778
Cuts at: 6296 6486 7346 2196
Size: 190 860 3354
Fragments arranged by size:
3354 986 934 860 778 570 335 230
190 153 114
Bsp1286I G_dGCh'C
Cuts at: 255 351 767 1521 2196 2310 3244 4230 4383
Size: 96 416 754 675 114 934 986 153
Cuts at: 4383 4953 5183 5518 6129 6222 6296 6486 6548
Size: 570 230 335 611 93 74 190 62
Cuts at: 6548 7346 255
Size: 798 1413
Fragments arranged by size:
1413 986 934 798 754 675 611 570
416 335 230 190 153 114 96 93
74 62
BspHI T'CATG_A
Cuts at: 507 5013 6936 507
Size: 4506 1923 2075
Fragments arranged by size:
4506 2075 1923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3622 4947 5554 5768 6070 6451 3622
Size: 1325 607 214 302 381 5675
Fragments arranged by size:
5675 1325 607 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6796 6850 8334 8499 50
Size: 6746 54 1484 165 55
Fragments arranged by size:
6746 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2407 2419 3475 3989 6416 2407
Size: 12 1056 514 2427 4495
Fragments arranged by size:
4495 2427 1056 514 12
BsrFI r'CCGG_y
Cuts at: 6502 6683 6502
Size: 181 8323
BsrGI T'GTAC_A
Cuts at: 2449 2449
Size: 8504
BssSI C'ACGA_G
Cuts at: 5475 6775 7483 5475
Size: 1300 708 6496
Fragments arranged by size:
6496 1300 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3614 5881 3614
Size: 2267 6237
BstBI TT'CG_AA
Cuts at: 6865 6865
Size: 8504
BstEII G'GTnAC_C
Cuts at: 1816 2160 4570 5280 1816
Size: 344 2410 710 5040
Fragments arranged by size:
5040 2410 710 344
BstXI CCAn_nnnn'nTGG
Cuts at: 114 2356 114
Size: 2242 6262
BstYI r'GATC_y
Cuts at: 1 1941 2478 2964 3178 3891 4737 5529 5996
Size: 1940 537 486 214 713 846 792 467
Cuts at: 5996 6019 6354 6600 6906 6918 7004 8056 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1940 1052 846 792 713 537 486 467
449 335 306 246 214 86 23 12
BstZ17I GTA'TAC
Cuts at: 3761 5005 3761
Size: 1244 7260
Bsu36I CC'TnA_GG
Cuts at: 932 932
Size: 8504
BtgI C'CryG_G
Cuts at: 107 107
Size: 8504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 139 6454 6635 6798 139
Size: 6315 181 163 1845
Fragments arranged by size:
6315 1845 181 163
BtsI GCAGTG_nn'
Cuts at: 1082 2900 5366 5402 5747 1082
Size: 1818 2466 36 345 3839
Fragments arranged by size:
3839 2466 1818 345 36
ClaI AT'CG_AT
Cuts at: 2864 2864
Size: 8504
DraI TTT'AAA
Cuts at: 153 1424 1620 4199 5503 153
Size: 1271 196 2579 1304 3154
Fragments arranged by size:
3154 2579 1304 1271 196
DrdI GACnn_nn'nnGTC
Cuts at: 6210 7554 6210
Size: 1344 7160
EaeI y'GGCC_r
Cuts at: 1392 2544 3365 5134 6089 6263 6654 6681 8026
Size: 1152 821 1769 955 174 391 27 1345
Cuts at: 8026 1392
Size: 1870
Fragments arranged by size:
1870 1769 1345 1152 955 821 391 174
27
EagI C'GGCC_G
Cuts at: 6089 6089
Size: 8504
EarI CTCTTCn'nnn_
Cuts at: 725 824 881 935 947 962 1850 3146 3350
Size: 99 57 54 12 15 888 1296 204
Cuts at: 3350 3476 3794 4017 4634 4718 5254 6527 6737
Size: 126 318 223 617 84 536 1273 210
Cuts at: 6737 725
Size: 2492
Fragments arranged by size:
2492 1296 1273 888 617 536 318 223
210 204 126 99 84 57 54 15
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6712 7454 7600 8314 6712
Size: 742 146 714 6902
Fragments arranged by size:
6902 742 714 146
EcoICRI GAG'CTC
Cuts at: 2308 4381 4951 5516 2308
Size: 2073 570 565 5296
Fragments arranged by size:
5296 2073 570 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 1085 1109 1143 2078 2581 3026 3194 3446
Size: 438 24 34 935 503 445 168 252
Cuts at: 3446 3722 3938 4037 4057 4579 4604 4615 4742
Size: 276 216 99 20 522 25 11 127
Cuts at: 4742 4829 5153 6329 6761 7114 7776 647
Size: 87 324 1176 432 353 662 1375
Fragments arranged by size:
1375 1176 935 662 522 503 445 438
432 353 324 276 252 216 168 127
99 87 34 25 24 20 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2948 2948
Size: 8504
EcoO109I rG'GnC_Cy
Cuts at: 667 5244 5643 667
Size: 4577 399 3528
Fragments arranged by size:
4577 3528 399
EcoRI G'AATT_C
Cuts at: 783 5508 783
Size: 4725 3779
EcoRV GAT'ATC
Cuts at: 2131 3297 2131
Size: 1166 7338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1405 1456 3418 4776 6523 1405
Size: 51 1962 1358 1747 3386
Fragments arranged by size:
3386 1962 1747 1358 51
FspI TGC'GCA
Cuts at: 6285 6285
Size: 8504
HaeII r_GCGC'y
Cuts at: 167 1441 3217 4821 5938 6186 7416 8187 167
Size: 1274 1776 1604 1117 248 1230 771 484
Fragments arranged by size:
1776 1604 1274 1230 1117 771 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 1107 1139 1576 1608 1827 1859 2091
Size: 32 573 32 437 32 219 32 232
Cuts at: 2091 2123 2292 2324 2614 2646 3434 3466 3655
Size: 32 169 32 290 32 788 32 189
Cuts at: 3655 3687 4273 4305 4789 4821 6346 6376 6378
Size: 32 586 32 484 32 1525 30 2
Cuts at: 6378 6408 502
Size: 30 2598
Fragments arranged by size:
2598 1525 788 586 573 484 437 290
232 219 189 169 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1262 2956 3081 3158 4758 5543 1262
Size: 1694 125 77 1600 785 4223
Fragments arranged by size:
4223 1694 1600 785 125 77
HindIII A'AGCT_T
Cuts at: 1418 1622 1661 1705 1925 2737 3815 4480 1418
Size: 204 39 44 220 812 1078 665 5442
Fragments arranged by size:
5442 1078 812 665 220 204 44 39
HpaI GTT'AAC
Cuts at: 1262 3158 1262
Size: 1896 6608
KasI G'GCGC_C
Cuts at: 5934 6182 5934
Size: 248 8256
KpnI G_GTAC'C
Cuts at: 5524 5524
Size: 8504
MfeI C'AATT_G
Cuts at: 1363 3655 4334 1363
Size: 2292 679 5533
Fragments arranged by size:
5533 2292 679
MluI A'CGCG_T
Cuts at: 7937 7937
Size: 8504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2070 2255 3686 4508 4521 5154 7263 7447 2070
Size: 185 1431 822 13 633 2109 184 3127
Fragments arranged by size:
3127 2109 1431 822 633 185 184 13
MscI TGG'CCA
Cuts at: 1394 2546 3367 6265 1394
Size: 1152 821 2898 3633
Fragments arranged by size:
3633 2898 1152 821
MslI CAynn'nnrTG
Cuts at: 112 185 593 1642 1784 2201 2321 2412 3560
Size: 73 408 1049 142 417 120 91 1148
Cuts at: 3560 4181 4892 5069 6493 6620 112
Size: 621 711 177 1424 127 1996
Fragments arranged by size:
1996 1424 1148 1049 711 621 417 408
177 142 127 120 91 73
MspA1I CmG'CkG
Cuts at: 2539 5615 5929 6289 7073 7318 2539
Size: 3076 314 360 784 245 3725
Fragments arranged by size:
3725 3076 784 360 314 245
NaeI GCC'GGC
Cuts at: 6685 6685
Size: 8504
NarI GG'CG_CC
Cuts at: 5935 6183 5935
Size: 248 8256
NcoI C'CATG_G
Cuts at: 107 107
Size: 8504
NdeI CA'TA_TG
Cuts at: 282 1119 1128 3230 3632 282
Size: 837 9 2102 402 5154
Fragments arranged by size:
5154 2102 837 402 9
NgoMIV G'CCGG_C
Cuts at: 6683 6683
Size: 8504
NheI G'CTAG_C
Cuts at: 4107 4107
Size: 8504
NsiI A_TGCA'T
Cuts at: 2376 2665 5066 2376
Size: 289 2401 5814
Fragments arranged by size:
5814 2401 289
NspI r_CATG'y
Cuts at: 592 2663 2722 2933 3201 3555 4148 5068 5500
Size: 2071 59 211 268 354 593 920 432
Cuts at: 5500 5557 6057 7660 592
Size: 57 500 1603 1436
Fragments arranged by size:
2071 1603 1436 920 593 500 432 354
268 211 59 57
PciI A'CATG_T
Cuts at: 4144 5496 7656 4144
Size: 1352 2160 4992
Fragments arranged by size:
4992 2160 1352
PflMI CCAn_nnn'nTGG
Cuts at: 718 718
Size: 8504
PfoI T'CCnGG_A
Cuts at: 663 3887 3915 4845 663
Size: 3224 28 930 4322
Fragments arranged by size:
4322 3224 930 28
PmeI GTTT'AAAC
Cuts at: 5503 5503
Size: 8504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6950 6982 6950
Size: 32 8472
PpuMI rG'GwC_Cy
Cuts at: 667 667
Size: 8504
PshAI GACnn'nnGTC
Cuts at: 4147 4147
Size: 8504
PsiI TTA'TAA
Cuts at: 2651 4906 2651
Size: 2255 6249
PspXI vC'TCGA_Gb
Cuts at: 5194 5194
Size: 8504
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4845 4877 4845
Size: 32 8472
PstI C_TGCA'G
Cuts at: 4416 5551 6236 4416
Size: 1135 685 6684
Fragments arranged by size:
6684 1135 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8504
PvuII CAG'CTG
Cuts at: 2539 5929 6289 2539
Size: 3390 360 4754
Fragments arranged by size:
4754 3390 360
RsrII CG'GwC_CG
Cuts at: 6699 6699
Size: 8504
SacI G_AGCT'C
Cuts at: 2310 4383 4953 5518 2310
Size: 2073 570 565 5296
Fragments arranged by size:
5296 2073 570 565
SalI G'TCGA_C
Cuts at: 5541 5541
Size: 8504
SapI GCTCTTCn'nnn_
Cuts at: 3794 4017 6527 6737 3794
Size: 223 2510 210 5561
Fragments arranged by size:
5561 2510 223 210
SbfI CC_TGCA'GG
Cuts at: 5551 5551
Size: 8504
ScaI AGT'ACT
Cuts at: 2599 4610 8021 2599
Size: 2011 3411 3082
Fragments arranged by size:
3411 3082 2011
SexAI A'CCwGG_T
Cuts at: 3775 3775
Size: 8504
SfcI C'TryA_G
Cuts at: 33 2389 4412 4785 5275 5547 6232 7200 7391
Size: 2356 2023 373 490 272 685 968 191
Cuts at: 7391 33
Size: 1146
Fragments arranged by size:
2356 2023 1146 968 685 490 373 272
191
SfoI GGC'GCC
Cuts at: 5936 6184 5936
Size: 248 8256
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8504
SmaI CCC'GGG
Cuts at: 5526 5526
Size: 8504
SmlI C'TyrA_G
Cuts at: 1520 1603 1888 4546 5108 5194 5369 5659 6574
Size: 83 285 2658 562 86 175 290 915
Cuts at: 6574 7288 7550 1520
Size: 714 262 2474
Fragments arranged by size:
2658 2474 915 714 562 290 285 262
175 86 83
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8504
SphI G_CATG'C
Cuts at: 2663 2933 3555 5557 6057 2663
Size: 270 622 2002 500 5110
Fragments arranged by size:
5110 2002 622 500 270
SspI AAT'ATT
Cuts at: 808 808
Size: 8504
StyI C'CwwG_G
Cuts at: 107 643 1389 2615 3064 5638 5992 107
Size: 536 746 1226 449 2574 354 2619
Fragments arranged by size:
2619 2574 1226 746 536 449 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2608 6087 6220 6679 6888 2608
Size: 3479 133 459 209 4224
Fragments arranged by size:
4224 3479 459 209 133
TatI w'GTAC_w
Cuts at: 2449 2597 4608 8019 2449
Size: 148 2011 3411 2934
Fragments arranged by size:
3411 2934 2011 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8021 8053 8021
Size: 32 8472
Tth111I GACn'n_nGTC
Cuts at: 1633 6301 1633
Size: 4668 3836
XbaI T'CTAG_A
Cuts at: 5310 5535 5310
Size: 225 8279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2613 5999 2613
Size: 3386 5118
XhoI C'TCGA_G
Cuts at: 5194 5194
Size: 8504
XmaI C'CCGG_G
Cuts at: 5524 5524
Size: 8504
XmnI GAAnn'nnTTC
Cuts at: 1325 2009 3590 4628 5102 1325
Size: 684 1581 1038 474 4727
Fragments arranged by size:
4727 1581 1038 684 474
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BmgBI Bpu10I BsiWI BsmI BsmBI
BspEI BssHII CspCI DraIII FseI FspAI NotI NruI
PacI PasI PmlI PspOMI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI