Restriction map for
pF1KA1432
(Circular) (Six-base) MAPSORT of: pF1KA1432.seq Check: 5295 from: 1 to: 5486
pF1KA1432 5486 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AccI GT'mk_AC
Cuts at: 1137 1523 2524 1137
Size: 386 1001 4099
Fragments arranged by size:
4099 1001 386
Acc65I G'GTAC_C
Cuts at: 2502 2502
Size: 5486
AclI AA'CG_TT
Cuts at: 5326 5326
Size: 5486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1227 1564 2102 3311 3743 4096 4758 1227
Size: 337 538 1209 432 353 662 1955
Fragments arranged by size:
1955 1209 662 538 432 353 337
AflIII A'CryG_T
Cuts at: 379 1379 2838 4638 4919 379
Size: 1000 1459 1800 281 946
Fragments arranged by size:
1800 1459 1000 946 281
AhdI GACnn_n'nnGTC
Cuts at: 380 380
Size: 5486
AlwNI CAG_nnn'CTG
Cuts at: 1367 2791 4229 1367
Size: 1424 1438 2624
Fragments arranged by size:
2624 1438 1424
ApaLI G'TGCA_C
Cuts at: 4324 4324
Size: 5486
ApoI r'AATT_y
Cuts at: 877 1297 1911 2490 4839 5095 877
Size: 420 614 579 2349 256 1268
Fragments arranged by size:
2349 1268 614 579 420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5486
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5486
AvaI C'yCGr_G
Cuts at: 1508 1517 2327 2506 1508
Size: 9 810 179 4488
Fragments arranged by size:
4488 810 179 9
AvrII C'CTAG_G
Cuts at: 472 472
Size: 5486
BamHI G'GATC_C
Cuts at: 2511 2511
Size: 5486
BanI G'GyrC_C
Cuts at: 1415 2036 2502 2916 3164 3199 1415
Size: 621 466 414 248 35 3702
Fragments arranged by size:
3702 621 466 414 248 35
BanII G_rGCy'C
Cuts at: 800 845 2162 2350 2500 3530 800
Size: 45 1317 188 150 1030 2756
Fragments arranged by size:
2756 1317 1030 188 150 45
BbeI G_GCGC'C
Cuts at: 1419 2920 3168 1419
Size: 1501 248 3737
Fragments arranged by size:
3737 1501 248
BbsI GAAGACnn'nnnn_
Cuts at: 937 2108 937
Size: 1171 4315
BciVI GTATCCnnnnn_n'
Cuts at: 3384 4440 5486 3384
Size: 1056 1046 3384
Fragments arranged by size:
3384 1056 1046
BclI T'GATC_A
Cuts at: 3006 3006
Size: 5486
BglII A'GATC_T
Cuts at: 686 3001 5038 686
Size: 2315 2037 1134
Fragments arranged by size:
2315 2037 1134
BlpI GC'TnA_GC
Cuts at: 427 1732 2598 427
Size: 1305 866 3315
Fragments arranged by size:
3315 1305 866
Bme1580I G_kGCm'C
Cuts at: 3111 3204 4328 3111
Size: 93 1124 4269
Fragments arranged by size:
4269 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 750 1211 1263 2698 2779 3114 5258 348
Size: 402 461 52 1435 81 335 2144 576
Fragments arranged by size:
2144 1435 576 461 402 335 81 52
BmtI G_CTAG'C
Cuts at: 550 550
Size: 5486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 696 698 1427 3853 696
Size: 2 729 2426 2329
Fragments arranged by size:
2426 2329 729 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 664 814 1193 1422 1966 2294 2337 433
Size: 231 150 379 229 544 328 43 3582
Fragments arranged by size:
3582 544 379 328 231 229 150 43
Bpu10I CC'TnA_GC
Cuts at: 1010 1208 1010
Size: 198 5288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2662 3541 4255 4553 1705
Size: 957 879 714 298 2638
Fragments arranged by size:
2638 957 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5190 5190
Size: 5486
BsaAI yAC'GTr
Cuts at: 2839 2839
Size: 5486
BsaBI GATnn'nnATC
Cuts at: 6 3000 5037 6
Size: 2994 2037 455
Fragments arranged by size:
2994 2037 455
BsaHI Gr'CG_yC
Cuts at: 1416 2917 3165 3867 5363 1416
Size: 1501 248 702 1496 1539
Fragments arranged by size:
1539 1501 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2675 3196 3874 4285 4432 4706 2675
Size: 521 678 411 147 274 3455
Fragments arranged by size:
3455 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2020 2050 2020
Size: 30 5456
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1009 2340 2350 2428 2449 1009
Size: 1331 10 78 21 4046
Fragments arranged by size:
4046 1331 78 21 10
BseYI C'CCAG_C
Cuts at: 506 1463 1467 1587 2911 3669 4334 4742 506
Size: 957 4 120 1324 758 665 408 1250
Fragments arranged by size:
1324 1250 957 758 665 408 120 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 461 233
Size: 228 5258
BsiEI CG_ry'CG
Cuts at: 104 3028 3074 4304 104
Size: 2924 46 1230 1286
Fragments arranged by size:
2924 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 653 800 1952 2500 3278 3468 4328 653
Size: 147 1152 548 778 190 860 1811
Fragments arranged by size:
1811 1152 860 778 548 190 147
BsmI GAATG_Cn'
Cuts at: 1840 1840
Size: 5486
Bsp1286I G_dGCh'C
Cuts at: 653 800 845 1952 2162 2350 2500 3111 3204
Size: 147 45 1107 210 188 150 611 93
Cuts at: 3204 3278 3468 3530 4328 653
Size: 74 190 62 798 1811
Fragments arranged by size:
1811 1107 798 611 210 190 188 150
147 93 74 62 45
BspHI T'CATG_A
Cuts at: 251 1755 3918 251
Size: 1504 2163 1819
Fragments arranged by size:
2163 1819 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2256 2536 2750 3052 3433 2256
Size: 280 214 302 381 4309
Fragments arranged by size:
4309 381 302 280 214
BsrBI CCG'CTC
Cuts at: 50 3778 3832 5316 5481 50
Size: 3728 54 1484 165 55
Fragments arranged by size:
3728 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3398 3398
Size: 5486
BsrFI r'CCGG_y
Cuts at: 3484 3665 3484
Size: 181 5305
BssSI C'ACGA_G
Cuts at: 206 3757 4465 206
Size: 3551 708 1227
Fragments arranged by size:
3551 1227 708
BstAPI GCAn_nnn'nTGC
Cuts at: 744 2863 744
Size: 2119 3367
BstBI TT'CG_AA
Cuts at: 3847 3847
Size: 5486
BstXI CCAn_nnnn'nTGG
Cuts at: 1965 1965
Size: 5486
BstYI r'GATC_y
Cuts at: 1 281 686 2511 2978 3001 3336 3582 3888
Size: 280 405 1825 467 23 335 246 306
Cuts at: 3888 3900 3986 5038 1
Size: 12 86 1052 449
Fragments arranged by size:
1825 1052 467 449 405 335 306 280
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 980 980
Size: 5486
BtgI C'CryG_G
Cuts at: 1779 1877 1958 2358 1779
Size: 98 81 400 4907
Fragments arranged by size:
4907 400 98 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3436 3617 3780 3436
Size: 181 163 5142
Fragments arranged by size:
5142 181 163
BtsI GCAGTG_nn'
Cuts at: 2174 2729 2174
Size: 555 4931
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1105 1140 1105
Size: 35 5451
DraI TTT'AAA
Cuts at: 2216 2485 2216
Size: 269 5217
DrdI GACnn_nn'nnGTC
Cuts at: 3192 4536 3192
Size: 1344 4142
EaeI y'GGCC_r
Cuts at: 1570 1967 3071 3245 3636 3663 5008 1570
Size: 397 1104 174 391 27 1345 2048
Fragments arranged by size:
2048 1345 1104 397 391 174 27
EagI C'GGCC_G
Cuts at: 3071 3071
Size: 5486
EarI CTCTTCn'nnn_
Cuts at: 472 658 904 1540 1599 2255 3509 3719 472
Size: 186 246 636 59 656 1254 210 2239
Fragments arranged by size:
2239 1254 656 636 246 210 186 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3694 4436 4582 5296 3694
Size: 742 146 714 3884
Fragments arranged by size:
3884 742 714 146
EcoICRI GAG'CTC
Cuts at: 798 2498 798
Size: 1700 3786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 664 814 1193 1227 1422 1564 1966 2102
Size: 231 150 379 34 195 142 402 136
Cuts at: 2102 2294 2337 3311 3743 4096 4758 433
Size: 192 43 974 432 353 662 1161
Fragments arranged by size:
1161 974 662 432 402 379 353 231
195 192 150 142 136 43 34
EcoNI CCTnn'n_nnAGG
Cuts at: 412 412
Size: 5486
EcoO109I rG'GnC_Cy
Cuts at: 417 1128 1443 1978 2625 417
Size: 711 315 535 647 3278
Fragments arranged by size:
3278 711 647 535 315
EcoRI G'AATT_C
Cuts at: 2490 2490
Size: 5486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3505 3505
Size: 5486
FspI TGC'GCA
Cuts at: 3267 3267
Size: 5486
HaeII r_GCGC'y
Cuts at: 1419 2920 3168 4398 5169 1419
Size: 1501 248 1230 771 1736
Fragments arranged by size:
1736 1501 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 233 265 366 398 3328 3358 3360 3390 233
Size: 32 101 32 2930 30 2 30 2329
Fragments arranged by size:
2930 2329 101 32 32 30 30 2
HincII GTy'rAC
Cuts at: 712 1138 2525 712
Size: 426 1387 3673
Fragments arranged by size:
3673 1387 426
KasI G'GCGC_C
Cuts at: 1415 2916 3164 1415
Size: 1501 248 3737
Fragments arranged by size:
3737 1501 248
KpnI G_GTAC'C
Cuts at: 2506 2506
Size: 5486
MluI A'CGCG_T
Cuts at: 4919 4919
Size: 5486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 951 1730 2310 2364 4245 4429 951
Size: 779 580 54 1881 184 2008
Fragments arranged by size:
2008 1881 779 580 184 54
MscI TGG'CCA
Cuts at: 1572 1969 3247 1572
Size: 397 1278 3811
Fragments arranged by size:
3811 1278 397
MslI CAynn'nnrTG
Cuts at: 253 1957 2023 3475 3602 253
Size: 1704 66 1452 127 2137
Fragments arranged by size:
2137 1704 1452 127 66
MspA1I CmG'CkG
Cuts at: 510 642 967 1027 1276 1467 1647 1666 2597
Size: 132 325 60 249 191 180 19 931
Cuts at: 2597 2911 3271 4055 4300 510
Size: 314 360 784 245 1696
Fragments arranged by size:
1696 931 784 360 325 314 249 245
191 180 132 60 19
NaeI GCC'GGC
Cuts at: 3667 3667
Size: 5486
NarI GG'CG_CC
Cuts at: 1416 2917 3165 1416
Size: 1501 248 3737
Fragments arranged by size:
3737 1501 248
NcoI C'CATG_G
Cuts at: 1779 1877 1958 2358 1779
Size: 98 81 400 4907
Fragments arranged by size:
4907 400 98 81
NgoMIV G'CCGG_C
Cuts at: 3665 3665
Size: 5486
NheI G'CTAG_C
Cuts at: 546 546
Size: 5486
NspI r_CATG'y
Cuts at: 1087 1383 1411 2539 3039 4642 1087
Size: 296 28 1128 500 1603 1931
Fragments arranged by size:
1931 1603 1128 500 296 28
PasI CC'CwG_GG
Cuts at: 2373 2373
Size: 5486
PciI A'CATG_T
Cuts at: 1379 4638 1379
Size: 3259 2227
PflMI CCAn_nnn'nTGG
Cuts at: 2169 2169
Size: 5486
PfoI T'CCnGG_A
Cuts at: 284 2196 284
Size: 1912 3574
PmeI GTTT'AAAC
Cuts at: 2485 2485
Size: 5486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 5454
PpuMI rG'GwC_Cy
Cuts at: 1128 1443 1128
Size: 315 5171
PshAI GACnn'nnGTC
Cuts at: 913 1133 913
Size: 220 5266
PstI C_TGCA'G
Cuts at: 908 1071 2240 2533 3218 908
Size: 163 1169 293 685 3176
Fragments arranged by size:
3176 1169 685 293 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5486
PvuII CAG'CTG
Cuts at: 642 967 1027 1276 1467 1647 1666 2911 3271
Size: 325 60 249 191 180 19 1245 360
Cuts at: 3271 642
Size: 2857
Fragments arranged by size:
2857 1245 360 325 249 191 180 60
19
RsrII CG'GwC_CG
Cuts at: 3681 3681
Size: 5486
SacI G_AGCT'C
Cuts at: 800 2500 800
Size: 1700 3786
SalI G'TCGA_C
Cuts at: 1136 2523 1136
Size: 1387 4099
SapI GCTCTTCn'nnn_
Cuts at: 658 2255 3509 3719 658
Size: 1597 1254 210 2425
Fragments arranged by size:
2425 1597 1254 210
SbfI CC_TGCA'GG
Cuts at: 2533 2533
Size: 5486
ScaI AGT'ACT
Cuts at: 815 5003 815
Size: 4188 1298
SfcI C'TryA_G
Cuts at: 33 904 1067 2186 2236 2529 3214 4182 4373
Size: 871 163 1119 50 293 685 968 191
Cuts at: 4373 33
Size: 1146
Fragments arranged by size:
1146 1119 968 871 685 293 191 163
50
SfoI GGC'GCC
Cuts at: 1417 2918 3166 1417
Size: 1501 248 3737
Fragments arranged by size:
3737 1501 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5486
SmaI CCC'GGG
Cuts at: 1519 2329 2508 1519
Size: 810 179 4497
Fragments arranged by size:
4497 810 179
SmlI C'TyrA_G
Cuts at: 1684 2641 3556 4270 4532 1684
Size: 957 915 714 262 2638
Fragments arranged by size:
2638 957 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5486
SphI G_CATG'C
Cuts at: 2539 3039 2539
Size: 500 4986
StuI AGG'CCT
Cuts at: 2063 2063
Size: 5486
StyI C'CwwG_G
Cuts at: 472 576 729 1446 1779 1877 1958 2358 2620
Size: 104 153 717 333 98 81 400 262
Cuts at: 2620 2974 472
Size: 354 2984
Fragments arranged by size:
2984 717 400 354 333 262 153 104
98 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 949 1215 1765 2189 3069 3202 3661 3870 949
Size: 266 550 424 880 133 459 209 2565
Fragments arranged by size:
2565 880 550 459 424 266 209 133
TatI w'GTAC_w
Cuts at: 166 345 813 5001 166
Size: 179 468 4188 651
Fragments arranged by size:
4188 651 468 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2020 2052 5003 5035 2020
Size: 32 2951 32 2471
Fragments arranged by size:
2951 2471 32 32
Tth111I GACn'n_nGTC
Cuts at: 3283 3283
Size: 5486
XbaI T'CTAG_A
Cuts at: 2517 2517
Size: 5486
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2981 2380
Size: 601 4885
XmaI C'CCGG_G
Cuts at: 1517 2327 2506 1517
Size: 810 179 4497
Fragments arranged by size:
4497 810 179
XmnI GAAnn'nnTTC
Cuts at: 1283 2447 1283
Size: 1164 4322
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI