Restriction map for
pF1KA1435
(Circular) (Six-base) MAPSORT of: pF1KA1435.seq Check: 2617 from: 1 to: 4343
pF1KA1435 4343 bp
With 182 enzymes: SgfI *
July 31, 2008 09:34 ..
AccI GT'mk_AC
Cuts at: 1381 1381
Size: 4343
Acc65I G'GTAC_C
Cuts at: 1359 1359
Size: 4343
AclI AA'CG_TT
Cuts at: 4183 4183
Size: 4343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 456 673 805 844 2168 2600 3347 3615
Size: 34 217 132 39 1324 432 747 268
Cuts at: 3615 422
Size: 1150
Fragments arranged by size:
1324 1150 747 432 268 217 132 39
34
AfeI AGC'GCT
Cuts at: 1093 1093
Size: 4343
AflIII A'CryG_T
Cuts at: 668 670 1220 1695 2799 3776 668
Size: 2 550 475 1104 977 1235
Fragments arranged by size:
1235 1104 977 550 475 2
AgeI A'CCGG_T
Cuts at: 2731 2731
Size: 4343
AlwNI CAG_nnn'CTG
Cuts at: 849 1648 3215 849
Size: 799 1567 1977
Fragments arranged by size:
1977 1567 799
ApaLI G'TGCA_C
Cuts at: 531 3113 531
Size: 2582 1761
ApoI r'AATT_y
Cuts at: 388 1347 3696 3952 388
Size: 959 2349 256 779
Fragments arranged by size:
2349 959 779 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
AvaI C'yCGr_G
Cuts at: 553 1363 553
Size: 810 3533
BamHI G'GATC_C
Cuts at: 1368 1368
Size: 4343
BanI G'GyrC_C
Cuts at: 173 1359 1773 2021 2056 173
Size: 1186 414 248 35 2460
Fragments arranged by size:
2460 1186 414 248 35
BanII G_rGCy'C
Cuts at: 1357 2387 1357
Size: 1030 3313
BbeI G_GCGC'C
Cuts at: 1777 2025 1777
Size: 248 4095
BciVI GTATCCnnnnn_n'
Cuts at: 2241 3002 4343 2241
Size: 761 1341 2241
Fragments arranged by size:
2241 1341 761
BclI T'GATC_A
Cuts at: 224 494 1863 224
Size: 270 1369 2704
Fragments arranged by size:
2704 1369 270
BglI GCCn_nnn'nGGC
Cuts at: 112 190 112
Size: 78 4265
BglII A'GATC_T
Cuts at: 1280 1858 3895 1280
Size: 578 2037 1728
Fragments arranged by size:
2037 1728 578
BlpI GC'TnA_GC
Cuts at: 157 1455 157
Size: 1298 3045
Bme1580I G_kGCm'C
Cuts at: 176 535 1968 2061 3117 176
Size: 359 1433 93 1056 1402
Fragments arranged by size:
1433 1402 1056 359 93
BmgBI CAC'GTC
Cuts at: 572 572
Size: 4343
BmrI ACTGGGnnnn_n'
Cuts at: 1099 1331 1555 1636 1971 4115 1099
Size: 232 224 81 335 2144 1327
Fragments arranged by size:
2144 1327 335 232 224 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 111
Size: 4343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1080 1519 2398 2890 3188 1080
Size: 439 879 492 298 2235
Fragments arranged by size:
2235 879 492 439 298
BsaI GGTCTCn'nnnn_
Cuts at: 4047 4047
Size: 4343
BsaAI yAC'GTr
Cuts at: 671 1696 671
Size: 1025 3318
BsaBI GATnn'nnATC
Cuts at: 6 1169 1857 3894 6
Size: 1163 688 2037 455
Fragments arranged by size:
2037 1163 688 455
BsaHI Gr'CG_yC
Cuts at: 182 1774 2022 2724 4220 182
Size: 1592 248 702 1496 305
Fragments arranged by size:
1592 1496 702 305 248
BsaWI w'CCGG_w
Cuts at: 1532 2053 2731 3005 3152 3563 1532
Size: 521 678 274 147 411 2312
Fragments arranged by size:
2312 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 932 932
Size: 4343
BseYI C'CCAG_C
Cuts at: 289 438 1026 1768 2526 3103 3599 289
Size: 149 588 742 758 577 496 1033
Fragments arranged by size:
1033 758 742 588 577 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1101 862
Size: 239 4104
BsiEI CG_ry'CG
Cuts at: 104 116 194 1269 1885 1931 3139 104
Size: 12 78 1075 616 46 1208 1308
Fragments arranged by size:
1308 1208 1075 616 78 46 12
BsiHKAI G_wGCw'C
Cuts at: 535 1357 2135 2325 3117 535
Size: 822 778 190 792 1761
Fragments arranged by size:
1761 822 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 885 885
Size: 4343
Bsp1286I G_dGCh'C
Cuts at: 176 535 1357 1968 2061 2135 2325 2387 3117
Size: 359 822 611 93 74 190 62 730
Cuts at: 3117 176
Size: 1402
Fragments arranged by size:
1402 822 730 611 359 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3563 3563
Size: 4343
BspHI T'CATG_A
Cuts at: 339 831 1194 3519 339
Size: 492 363 2325 1163
Fragments arranged by size:
2325 1163 492 363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1393 1607 1909 2290 1393
Size: 214 302 381 3446
Fragments arranged by size:
3446 381 302 214
BsrBI CCG'CTC
Cuts at: 50 542 2635 2689 4173 4338 50
Size: 492 2093 54 1484 165 55
Fragments arranged by size:
2093 1484 492 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1232 2255 1232
Size: 1023 3320
BsrFI r'CCGG_y
Cuts at: 502 2341 2522 2731 502
Size: 1839 181 209 2114
Fragments arranged by size:
2114 1839 209 181
BssSI C'ACGA_G
Cuts at: 2614 2972 2614
Size: 358 3985
BstAPI GCAn_nnn'nTGC
Cuts at: 1055 1720 1055
Size: 665 3678
BstBI TT'CG_AA
Cuts at: 2704 2704
Size: 4343
BstEII G'GTnAC_C
Cuts at: 1251 1251
Size: 4343
BstXI CCAn_nnnn'nTGG
Cuts at: 373 992 1026 373
Size: 619 34 3690
Fragments arranged by size:
3690 619 34
BstYI r'GATC_y
Cuts at: 1 1280 1368 1835 1858 2193 2439 3451 3537
Size: 1279 88 467 23 335 246 1012 86
Cuts at: 3537 3549 3895 1
Size: 12 346 449
Fragments arranged by size:
1279 1012 467 449 346 335 246 88
86 23 12
BtgI C'CryG_G
Cuts at: 107 1226 107
Size: 1119 3224
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2293 2474 2637 2293
Size: 181 163 3999
Fragments arranged by size:
3999 181 163
BtsI GCAGTG_nn'
Cuts at: 914 1044 1586 914
Size: 130 542 3671
Fragments arranged by size:
3671 542 130
DraI TTT'AAA
Cuts at: 1342 1342
Size: 4343
DraIII CAC_nnn'GTG
Cuts at: 488 488
Size: 4343
DrdI GACnn_nn'nnGTC
Cuts at: 2049 2907 2049
Size: 858 3485
EaeI y'GGCC_r
Cuts at: 113 191 230 479 504 1928 2102 2493 2520
Size: 78 39 249 25 1424 174 391 27
Cuts at: 2520 3865 113
Size: 1345 591
Fragments arranged by size:
1424 1345 591 391 249 174 78 39
27 25
EagI C'GGCC_G
Cuts at: 113 191 1928 113
Size: 78 1737 2528
Fragments arranged by size:
2528 1737 78
EarI CTCTTCn'nnn_
Cuts at: 754 929 2366 2576 754
Size: 175 1437 210 2521
Fragments arranged by size:
2521 1437 210 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 914 2551 2861 3007 4153 914
Size: 1637 310 146 1146 1104
Fragments arranged by size:
1637 1146 1104 310 146
EcoICRI GAG'CTC
Cuts at: 1355 1355
Size: 4343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 422 456 673 805 844 2168 2600 3347
Size: 311 34 217 132 39 1324 432 747
Cuts at: 3347 3615 111
Size: 268 839
Fragments arranged by size:
1324 839 747 432 311 268 217 132
39 34
EcoO109I rG'GnC_Cy
Cuts at: 729 1482 729
Size: 753 3590
EcoRI G'AATT_C
Cuts at: 1347 1347
Size: 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 980 2362 980
Size: 1382 2961
FspI TGC'GCA
Cuts at: 2124 2124
Size: 4343
HaeII r_GCGC'y
Cuts at: 1095 1777 2025 3047 4026 1095
Size: 682 248 1022 979 1412
Fragments arranged by size:
1412 1022 979 682 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2185 2215 2217 2247 2185
Size: 30 2 30 4281
Fragments arranged by size:
4281 30 30 2
HincII GTy'rAC
Cuts at: 1382 1382
Size: 4343
HindIII A'AGCT_T
Cuts at: 655 655
Size: 4343
KasI G'GCGC_C
Cuts at: 1773 2021 1773
Size: 248 4095
KpnI G_GTAC'C
Cuts at: 1363 1363
Size: 4343
MluI A'CGCG_T
Cuts at: 3776 3776
Size: 4343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 932 3014 3198 932
Size: 2082 184 2077
Fragments arranged by size:
2082 2077 184
MscI TGG'CCA
Cuts at: 481 2104 481
Size: 1623 2720
MslI CAynn'nnrTG
Cuts at: 778 1225 2332 2459 778
Size: 447 1107 127 2662
Fragments arranged by size:
2662 1107 447 127
MspA1I CmG'CkG
Cuts at: 741 1454 1768 2128 3141 3386 741
Size: 713 314 360 1013 245 1698
Fragments arranged by size:
1698 1013 713 360 314 245
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 4343
NarI GG'CG_CC
Cuts at: 1774 2022 1774
Size: 248 4095
NcoI C'CATG_G
Cuts at: 107 1226 107
Size: 1119 3224
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 4343
NspI r_CATG'y
Cuts at: 552 1224 1396 1896 2803 552
Size: 672 172 500 907 2092
Fragments arranged by size:
2092 907 672 500 172
PciI A'CATG_T
Cuts at: 1220 2799 1220
Size: 1579 2764
PflMI CCAn_nnn'nTGG
Cuts at: 306 488 306
Size: 182 4161
PmeI GTTT'AAAC
Cuts at: 1342 1342
Size: 4343
PmlI CAC'GTG
Cuts at: 671 671
Size: 4343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 4311
PpuMI rG'GwC_Cy
Cuts at: 729 729
Size: 4343
PshAI GACnn'nnGTC
Cuts at: 275 275
Size: 4343
PstI C_TGCA'G
Cuts at: 1051 1312 1390 2075 1051
Size: 261 78 685 3319
Fragments arranged by size:
3319 685 261 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4343
PvuII CAG'CTG
Cuts at: 1768 2128 1768
Size: 360 3983
RsrII CG'GwC_CG
Cuts at: 2538 2538
Size: 4343
SacI G_AGCT'C
Cuts at: 1357 1357
Size: 4343
SalI G'TCGA_C
Cuts at: 1380 1380
Size: 4343
SanDI GG'GwC_CC
Cuts at: 729 729
Size: 4343
SapI GCTCTTCn'nnn_
Cuts at: 2366 2576 2366
Size: 210 4133
SbfI CC_TGCA'GG
Cuts at: 1390 1390
Size: 4343
ScaI AGT'ACT
Cuts at: 3860 3860
Size: 4343
SfcI C'TryA_G
Cuts at: 33 1047 1308 1386 2071 3064 3255 33
Size: 1014 261 78 685 993 191 1121
Fragments arranged by size:
1121 1014 993 685 261 191 78
SfoI GGC'GCC
Cuts at: 1775 2023 1775
Size: 248 4095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
SmaI CCC'GGG
Cuts at: 1365 1365
Size: 4343
SmlI C'TyrA_G
Cuts at: 1095 1498 2413 2905 3167 1095
Size: 403 915 492 262 2271
Fragments arranged by size:
2271 915 492 403 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4343
SphI G_CATG'C
Cuts at: 1396 1896 1396
Size: 500 3843
StyI C'CwwG_G
Cuts at: 107 209 1226 1477 1831 107
Size: 102 1017 251 354 2619
Fragments arranged by size:
2619 1017 354 251 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 480 1279 1926 2059 2518 2727 480
Size: 799 647 133 459 209 2096
Fragments arranged by size:
2096 799 647 459 209 133
TatI w'GTAC_w
Cuts at: 3858 3858
Size: 4343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 893 925 1235 1267 3860 3892 893
Size: 32 310 32 2593 32 1344
Fragments arranged by size:
2593 1344 310 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 184 2140 184
Size: 1956 2387
XbaI T'CTAG_A
Cuts at: 1374 1374
Size: 4343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1245 1838 1245
Size: 593 3750
XmaI C'CCGG_G
Cuts at: 1363 1363
Size: 4343
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI