Restriction map for
pF1KA1448
(Circular) (Six-base) MAPSORT of: pF1KA1448.seq Check: 5138 from: 1 to: 6572
pF1KA1448 6572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1336 2483 2525 2862 1336
Size: 1147 42 337 5046
Fragments arranged by size:
5046 1147 337 42
AccI GT'mk_AC
Cuts at: 1366 3610 1366
Size: 2244 4328
Acc65I G'GTAC_C
Cuts at: 3588 3588
Size: 6572
AclI AA'CG_TT
Cuts at: 1395 6412 1395
Size: 5017 1555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1246 2346 4397 4829 5576 5844 240
Size: 1006 1100 2051 432 747 268 968
Fragments arranged by size:
2051 1100 1006 968 747 432 268
AfeI AGC'GCT
Cuts at: 1774 1774
Size: 6572
AflII C'TTAA_G
Cuts at: 2646 2646
Size: 6572
AflIII A'CryG_T
Cuts at: 2814 3924 5028 6005 2814
Size: 1110 1104 977 3381
Fragments arranged by size:
3381 1110 1104 977
AgeI A'CCGG_T
Cuts at: 4960 4960
Size: 6572
AhdI GACnn_n'nnGTC
Cuts at: 2121 2121
Size: 6572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 364 422 364
Size: 58 6514
AlwNI CAG_nnn'CTG
Cuts at: 1251 1657 2082 3485 3877 5444 1251
Size: 406 425 1403 392 1567 2379
Fragments arranged by size:
2379 1567 1403 425 406 392
ApaLI G'TGCA_C
Cuts at: 5342 5342
Size: 6572
ApoI r'AATT_y
Cuts at: 551 1041 2315 2885 3576 5925 6181 551
Size: 490 1274 570 691 2349 256 942
Fragments arranged by size:
2349 1274 942 691 570 490 256
AseI AT'TA_AT
Cuts at: 20 920 3221 20
Size: 900 2301 3371
Fragments arranged by size:
3371 2301 900
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6572
AvaI C'yCGr_G
Cuts at: 158 3592 158
Size: 3434 3138
AvrII C'CTAG_G
Cuts at: 1536 1536
Size: 6572
BamHI G'GATC_C
Cuts at: 2838 3150 3597 2838
Size: 312 447 5813
Fragments arranged by size:
5813 447 312
BanI G'GyrC_C
Cuts at: 883 1664 3588 4002 4250 4285 883
Size: 781 1924 414 248 35 3170
Fragments arranged by size:
3170 1924 781 414 248 35
BanII G_rGCy'C
Cuts at: 1087 1692 1766 3172 3586 4616 1087
Size: 605 74 1406 414 1030 3043
Fragments arranged by size:
3043 1406 1030 605 414 74
BbeI G_GCGC'C
Cuts at: 1668 4006 4254 1668
Size: 2338 248 3986
Fragments arranged by size:
3986 2338 248
BbsI GAAGACnn'nnnn_
Cuts at: 1592 1912 2525 1592
Size: 320 613 5639
Fragments arranged by size:
5639 613 320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2914 2948 2914
Size: 34 6538
BciVI GTATCCnnnnn_n'
Cuts at: 2481 4470 5231 6572 2481
Size: 1989 761 1341 2481
Fragments arranged by size:
2481 1989 1341 761
BclI T'GATC_A
Cuts at: 4092 4092
Size: 6572
BfrBI ATG'CAT
Cuts at: 185 185
Size: 6572
BglI GCCn_nnn'nGGC
Cuts at: 455 455
Size: 6572
BglII A'GATC_T
Cuts at: 2027 2058 2307 4087 6124 2027
Size: 31 249 1780 2037 2475
Fragments arranged by size:
2475 2037 1780 249 31
BlpI GC'TnA_GC
Cuts at: 1658 1681 1895 3684 1658
Size: 23 214 1789 4546
Fragments arranged by size:
4546 1789 214 23
Bme1580I G_kGCm'C
Cuts at: 4197 4290 5346 4197
Size: 93 1056 5423
Fragments arranged by size:
5423 1056 93
BmgBI CAC'GTC
Cuts at: 2854 2854
Size: 6572
BmrI ACTGGGnnnn_n'
Cuts at: 2543 3150 3784 3865 4200 6344 2543
Size: 607 634 81 335 2144 2771
Fragments arranged by size:
2771 2144 634 607 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1706 1944 2472 4939 1706
Size: 238 528 2467 3339
Fragments arranged by size:
3339 2467 528 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 2481 3443 1394
Size: 1087 962 4523
Fragments arranged by size:
4523 1087 962
Bpu10I CC'TnA_GC
Cuts at: 1244 1827 3070 1244
Size: 583 1243 4746
Fragments arranged by size:
4746 1243 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2295 2548 3158 3748 4627 5119 5417 2295
Size: 253 610 590 879 492 298 3450
Fragments arranged by size:
3450 879 610 590 492 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 157 284 799 1915 6276 157
Size: 127 515 1116 4361 453
Fragments arranged by size:
4361 1116 515 453 127
BsaAI yAC'GTr
Cuts at: 1788 3925 1788
Size: 2137 4435
BsaBI GATnn'nnATC
Cuts at: 6 4086 6123 6
Size: 4080 2037 455
Fragments arranged by size:
4080 2037 455
BsaHI Gr'CG_yC
Cuts at: 1333 1665 2480 2522 2859 4003 4251 4953 6449
Size: 332 815 42 337 1144 248 702 1496
Cuts at: 6449 1333
Size: 1456
Fragments arranged by size:
1496 1456 1144 815 702 337 332 248
42
BsaWI w'CCGG_w
Cuts at: 1718 2972 3761 4282 4960 5234 5381 5792 1718
Size: 1254 789 521 678 274 147 411 2498
Fragments arranged by size:
2498 1254 789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 634 664 107
Size: 30 497 30 6015
Fragments arranged by size:
6015 497 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1225 1391 2647 3531 1225
Size: 166 1256 884 4266
Fragments arranged by size:
4266 1256 884 166
BseYI C'CCAG_C
Cuts at: 700 1808 2176 2822 3478 3997 4755 5332 5828
Size: 1108 368 646 656 519 758 577 496
Cuts at: 5828 700
Size: 1444
Fragments arranged by size:
1444 1108 758 656 646 577 519 496
368
BsiEI CG_ry'CG
Cuts at: 104 4114 4160 5368 104
Size: 4010 46 1208 1308
Fragments arranged by size:
4010 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1245 3586 4364 4554 5346 1120
Size: 125 2341 778 190 792 2346
Fragments arranged by size:
2346 2341 792 778 190 125
Bsp1286I G_dGCh'C
Cuts at: 1087 1120 1245 1692 1766 3172 3586 4197 4290
Size: 33 125 447 74 1406 414 611 93
Cuts at: 4290 4364 4554 4616 5346 1087
Size: 74 190 62 730 2313
Fragments arranged by size:
2313 1406 730 611 447 414 190 125
93 74 74 62 33
BspEI T'CCGG_A
Cuts at: 1718 5792 1718
Size: 4074 2498
BspHI T'CATG_A
Cuts at: 5748 5748
Size: 6572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3622 3836 4138 4519 3622
Size: 214 302 381 5675
Fragments arranged by size:
5675 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1662 1685 2451 4864 4918 6402 6567 50
Size: 1612 23 766 2413 54 1484 165 55
Fragments arranged by size:
2413 1612 1484 766 165 55 54 23
BsrDI GCAATG_nn'
Cuts at: 680 1073 1164 1418 3447 4484 680
Size: 393 91 254 2029 1037 2768
Fragments arranged by size:
2768 2029 1037 393 254 91
BsrFI r'CCGG_y
Cuts at: 4570 4751 4960 4570
Size: 181 209 6182
Fragments arranged by size:
6182 209 181
BsrGI T'GTAC_A
Cuts at: 326 3357 326
Size: 3031 3541
BssSI C'ACGA_G
Cuts at: 1889 4843 5201 1889
Size: 2954 358 3260
Fragments arranged by size:
3260 2954 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1138 3949 1072
Size: 66 2811 3695
Fragments arranged by size:
3695 2811 66
BstBI TT'CG_AA
Cuts at: 4933 4933
Size: 6572
BstEII G'GTnAC_C
Cuts at: 3340 3340
Size: 6572
BstXI CCAn_nnnn'nTGG
Cuts at: 720 1476 720
Size: 756 5816
BstYI r'GATC_y
Cuts at: 1 297 645 849 2027 2058 2307 2692 2838
Size: 296 348 204 1178 31 249 385 146
Cuts at: 2838 3150 3597 4064 4087 4422 4668 5680 5766
Size: 312 447 467 23 335 246 1012 86
Cuts at: 5766 5778 6124 1
Size: 12 346 449
Fragments arranged by size:
1178 1012 467 449 447 385 348 346
335 312 296 249 246 204 146 86
31 23 12
BtgI C'CryG_G
Cuts at: 1088 1274 3163 3314 3508 1088
Size: 186 1889 151 194 4152
Fragments arranged by size:
4152 1889 194 186 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4522 4703 4866 4522
Size: 181 163 6228
Fragments arranged by size:
6228 181 163
BtsI GCAGTG_nn'
Cuts at: 2226 2474 3347 3815 2226
Size: 248 873 468 4983
Fragments arranged by size:
4983 873 468 248
DraI TTT'AAA
Cuts at: 1400 3571 1400
Size: 2171 4401
DraIII CAC_nnn'GTG
Cuts at: 765 765
Size: 6572
DrdI GACnn_nn'nnGTC
Cuts at: 2858 4278 5136 2858
Size: 1420 858 4294
Fragments arranged by size:
4294 1420 858
EaeI y'GGCC_r
Cuts at: 962 1644 2393 2471 2528 3276 4157 4331 4722
Size: 682 749 78 57 748 881 174 391
Cuts at: 4722 4749 6094 962
Size: 27 1345 1440
Fragments arranged by size:
1440 1345 881 749 748 682 391 174
78 57 27
EagI C'GGCC_G
Cuts at: 4157 4157
Size: 6572
EarI CTCTTCn'nnn_
Cuts at: 1439 1697 2126 3107 4595 4805 1439
Size: 258 429 981 1488 210 3206
Fragments arranged by size:
3206 1488 981 429 258 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4780 5090 5236 6382 4780
Size: 310 146 1146 4970
Fragments arranged by size:
4970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3584 3584
Size: 6572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1246 1394 2346 2481 3443 4397 4829 5576
Size: 1006 148 952 135 962 954 432 747
Cuts at: 5576 5844 240
Size: 268 968
Fragments arranged by size:
1006 968 962 954 952 747 432 268
148 135
EcoO109I rG'GnC_Cy
Cuts at: 146 1176 1230 1248 1280 3711 146
Size: 1030 54 18 32 2431 3007
Fragments arranged by size:
3007 2431 1030 54 32 18
EcoRI G'AATT_C
Cuts at: 3576 3576
Size: 6572
EcoRV GAT'ATC
Cuts at: 1095 1095
Size: 6572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1468 2077 3000 4591 1468
Size: 609 923 1591 3449
Fragments arranged by size:
3449 1591 923 609
FspI TGC'GCA
Cuts at: 4353 4353
Size: 6572
HaeII r_GCGC'y
Cuts at: 1668 1776 2274 3381 4006 4254 5276 6255 1668
Size: 108 498 1107 625 248 1022 979 1985
Fragments arranged by size:
1985 1107 1022 979 625 498 248 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 362 394 634 666 1551 1583 2107 2139 2171
Size: 32 240 32 885 32 524 32 32
Cuts at: 2171 2679 2711 4414 4444 4446 4476 362
Size: 508 32 1703 30 2 30 2458
Fragments arranged by size:
2458 1703 885 524 508 240 32 32
32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1575 3611 1575
Size: 2036 4536
HindIII A'AGCT_T
Cuts at: 1450 1450
Size: 6572
HpaI GTT'AAC
Cuts at: 1575 1575
Size: 6572
KasI G'GCGC_C
Cuts at: 1664 4002 4250 1664
Size: 2338 248 3986
Fragments arranged by size:
3986 2338 248
KpnI G_GTAC'C
Cuts at: 3592 3592
Size: 6572
MluI A'CGCG_T
Cuts at: 6005 6005
Size: 6572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3232 5243 5427 3232
Size: 2011 184 4377
Fragments arranged by size:
4377 2011 184
MscI TGG'CCA
Cuts at: 1646 2395 2473 2530 3278 4333 1646
Size: 749 78 57 748 1055 3885
Fragments arranged by size:
3885 1055 749 748 78 57
MslI CAynn'nnrTG
Cuts at: 193 471 800 1474 4561 4688 193
Size: 278 329 674 3087 127 2077
Fragments arranged by size:
3087 2077 674 329 278 127
MspA1I CmG'CkG
Cuts at: 1090 1739 1821 2826 3316 3510 3683 3997 4357
Size: 649 82 1005 490 194 173 314 360
Cuts at: 4357 5370 5615 1090
Size: 1013 245 2047
Fragments arranged by size:
2047 1013 1005 649 490 360 314 245
194 173 82
NaeI GCC'GGC
Cuts at: 4753 4753
Size: 6572
NarI GG'CG_CC
Cuts at: 1665 4003 4251 1665
Size: 2338 248 3986
Fragments arranged by size:
3986 2338 248
NcoI C'CATG_G
Cuts at: 1274 3163 1274
Size: 1889 4683
NgoMIV G'CCGG_C
Cuts at: 4751 4751
Size: 6572
NsiI A_TGCA'T
Cuts at: 187 187
Size: 6572
NspI r_CATG'y
Cuts at: 3625 4125 5032 3625
Size: 500 907 5165
Fragments arranged by size:
5165 907 500
PciI A'CATG_T
Cuts at: 5028 5028
Size: 6572
PflMI CCAn_nnn'nTGG
Cuts at: 628 2221 628
Size: 1593 4979
PfoI T'CCnGG_A
Cuts at: 2458 2458
Size: 6572
PmeI GTTT'AAAC
Cuts at: 3571 3571
Size: 6572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 5711
Size: 32 6540
PpuMI rG'GwC_Cy
Cuts at: 1176 1230 1280 1176
Size: 54 50 6468
Fragments arranged by size:
6468 54 50
PsiI TTA'TAA
Cuts at: 3416 3416
Size: 6572
PstI C_TGCA'G
Cuts at: 3619 4304 3619
Size: 685 5887
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6572
PvuII CAG'CTG
Cuts at: 1821 2826 3997 4357 1821
Size: 1005 1171 360 4036
Fragments arranged by size:
4036 1171 1005 360
RsrII CG'GwC_CG
Cuts at: 4767 4767
Size: 6572
SacI G_AGCT'C
Cuts at: 3586 3586
Size: 6572
SacII CC_GC'GG
Cuts at: 1091 3317 3511 1091
Size: 2226 194 4152
Fragments arranged by size:
4152 2226 194
SalI G'TCGA_C
Cuts at: 3609 3609
Size: 6572
SanDI GG'GwC_CC
Cuts at: 1280 1280
Size: 6572
SapI GCTCTTCn'nnn_
Cuts at: 1697 4595 4805 1697
Size: 2898 210 3464
Fragments arranged by size:
3464 2898 210
SbfI CC_TGCA'GG
Cuts at: 3619 3619
Size: 6572
ScaI AGT'ACT
Cuts at: 6089 6089
Size: 6572
SfcI C'TryA_G
Cuts at: 33 530 1005 2014 3486 3615 4300 5293 5484
Size: 497 475 1009 1472 129 685 993 191
Cuts at: 5484 33
Size: 1121
Fragments arranged by size:
1472 1121 1009 993 685 497 475 191
129
SfoI GGC'GCC
Cuts at: 1666 4004 4252 1666
Size: 2338 248 3986
Fragments arranged by size:
3986 2338 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6572
SmaI CCC'GGG
Cuts at: 3594 3594
Size: 6572
SmlI C'TyrA_G
Cuts at: 158 2274 2563 2646 3173 3727 4642 5134 5396
Size: 2116 289 83 527 554 915 492 262
Cuts at: 5396 158
Size: 1334
Fragments arranged by size:
2116 1334 915 554 527 492 289 262
83
SnaBI TAC'GTA
Cuts at: 1788 1788
Size: 6572
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6572
SphI G_CATG'C
Cuts at: 3625 4125 3625
Size: 500 6072
SspI AAT'ATT
Cuts at: 918 918
Size: 6572
StyI C'CwwG_G
Cuts at: 959 1274 1536 3163 3706 4060 959
Size: 315 262 1627 543 354 3471
Fragments arranged by size:
3471 1627 543 354 315 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 635 799 3549 4155 4288 4747 4956 635
Size: 164 2750 606 133 459 209 2251
Fragments arranged by size:
2750 2251 606 459 209 164 133
TatI w'GTAC_w
Cuts at: 326 1017 3357 6087 326
Size: 691 2340 2730 811
Fragments arranged by size:
2730 2340 811 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 107 139 623 655 2217 2249 6089 6121 107
Size: 32 484 32 1562 32 3840 32 558
Fragments arranged by size:
3840 1562 558 484 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4369 4369
Size: 6572
XbaI T'CTAG_A
Cuts at: 3603 3603
Size: 6572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1188 2830 4067 1188
Size: 1642 1237 3693
Fragments arranged by size:
3693 1642 1237
XhoI C'TCGA_G
Cuts at: 158 158
Size: 6572
XmaI C'CCGG_G
Cuts at: 3592 3592
Size: 6572
XmnI GAAnn'nnTTC
Cuts at: 3261 3261
Size: 6572
ZraI GAC'GTC
Cuts at: 1334 2481 2523 2860 1334
Size: 1147 42 337 5046
Fragments arranged by size:
5046 1147 337 42
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AleI AloI ApaI AscI BaeI BbvCI BmtI
BsgI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI EcoNI FseI FspAI MfeI NdeI NheI NotI
NruI PacI PasI PmlI PshAI PspOMI PspXI PsrI
SexAI SfiI SgrAI SrfI StuI SwaI