Restriction map for
pF1KA1451
(Circular) (Six-base) MAPSORT of: pF1KA1451.seq Check: 9212 from: 1 to: 5780
pF1KA1451 5780 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1267 1267
Size: 5780
AccI GT'mk_AC
Cuts at: 2818 2818
Size: 5780
Acc65I G'GTAC_C
Cuts at: 2796 2796
Size: 5780
AclI AA'CG_TT
Cuts at: 5620 5620
Size: 5780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 1328 1567 2228 3605 4037 4784 5052 836
Size: 492 239 661 1377 432 747 268 1564
Fragments arranged by size:
1564 1377 747 661 492 432 268 239
AflII C'TTAA_G
Cuts at: 469 469
Size: 5780
AflIII A'CryG_T
Cuts at: 978 1888 3132 4236 5213 978
Size: 910 1244 1104 977 1545
Fragments arranged by size:
1545 1244 1104 977 910
AgeI A'CCGG_T
Cuts at: 4168 4168
Size: 5780
AleI CACnn'nnGTG
Cuts at: 979 1022 979
Size: 43 5737
AlwNI CAG_nnn'CTG
Cuts at: 219 1300 2512 3085 4652 219
Size: 1081 1212 573 1567 1347
Fragments arranged by size:
1567 1347 1212 1081 573
ApaLI G'TGCA_C
Cuts at: 4550 4550
Size: 5780
ApoI r'AATT_y
Cuts at: 1838 1921 2128 2784 5133 5389 1838
Size: 83 207 656 2349 256 2229
Fragments arranged by size:
2349 2229 656 256 207 83
AseI AT'TA_AT
Cuts at: 20 2110 20
Size: 2090 3690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5780
AvaI C'yCGr_G
Cuts at: 2180 2800 2180
Size: 620 5160
AvrII C'CTAG_G
Cuts at: 1941 1941
Size: 5780
BamHI G'GATC_C
Cuts at: 802 2805 802
Size: 2003 3777
BanI G'GyrC_C
Cuts at: 2796 3210 3458 3493 2796
Size: 414 248 35 5083
Fragments arranged by size:
5083 414 248 35
BanII G_rGCy'C
Cuts at: 2794 3824 2794
Size: 1030 4750
BbeI G_GCGC'C
Cuts at: 3214 3462 3214
Size: 248 5532
BbsI GAAGACnn'nnnn_
Cuts at: 904 904
Size: 5780
BciVI GTATCCnnnnn_n'
Cuts at: 1723 1897 3678 4439 5780 1723
Size: 174 1781 761 1341 1723
Fragments arranged by size:
1781 1723 1341 761 174
BclI T'GATC_A
Cuts at: 836 929 1113 3300 836
Size: 93 184 2187 3316
Fragments arranged by size:
3316 2187 184 93
BglI GCCn_nnn'nGGC
Cuts at: 2469 2469
Size: 5780
BglII A'GATC_T
Cuts at: 3295 5332 3295
Size: 2037 3743
BlpI GC'TnA_GC
Cuts at: 2892 2892
Size: 5780
Bme1580I G_kGCm'C
Cuts at: 3405 3498 4554 3405
Size: 93 1056 4631
Fragments arranged by size:
4631 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1996 2596 2992 3073 3408 5552 1996
Size: 600 396 81 335 2144 2224
Fragments arranged by size:
2224 2144 600 396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4147 4147
Size: 5780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 1882
Size: 5780
Bpu10I CC'TnA_GC
Cuts at: 955 1211 1702 955
Size: 256 491 5033
Fragments arranged by size:
5033 491 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2956 3835 4327 4625 2956
Size: 879 492 298 4111
Fragments arranged by size:
4111 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5484 5484
Size: 5780
BsaAI yAC'GTr
Cuts at: 1003 3133 1003
Size: 2130 3650
BsaBI GATnn'nnATC
Cuts at: 6 284 3294 5331 6
Size: 278 3010 2037 455
Fragments arranged by size:
3010 2037 455 278
BsaHI Gr'CG_yC
Cuts at: 3211 3459 4161 5657 3211
Size: 248 702 1496 3334
Fragments arranged by size:
3334 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2969 3490 4168 4442 4589 5000 2969
Size: 521 678 274 147 411 3749
Fragments arranged by size:
3749 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2517 2517
Size: 5780
BseYI C'CCAG_C
Cuts at: 3205 3963 4540 5036 3205
Size: 758 577 496 3949
Fragments arranged by size:
3949 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3322 3368 4576 104
Size: 3218 46 1208 1308
Fragments arranged by size:
3218 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2794 3572 3762 4554 2794
Size: 778 190 792 4020
Fragments arranged by size:
4020 792 778 190
BsmI GAATG_Cn'
Cuts at: 693 693
Size: 5780
Bsp1286I G_dGCh'C
Cuts at: 2794 3405 3498 3572 3762 3824 4554 2794
Size: 611 93 74 190 62 730 4020
Fragments arranged by size:
4020 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5000 5000
Size: 5780
BspHI T'CATG_A
Cuts at: 4956 4956
Size: 5780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1267 2830 3044 3346 3727 1267
Size: 1563 214 302 381 3320
Fragments arranged by size:
3320 1563 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4072 4126 5610 5775 50
Size: 4022 54 1484 165 55
Fragments arranged by size:
4022 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2107 3692 2107
Size: 1585 4195
BsrFI r'CCGG_y
Cuts at: 2685 3778 3959 4168 2685
Size: 1093 181 209 4297
Fragments arranged by size:
4297 1093 209 181
BssSI C'ACGA_G
Cuts at: 4051 4409 4051
Size: 358 5422
BstAPI GCAn_nnn'nTGC
Cuts at: 3157 3157
Size: 5780
BstBI TT'CG_AA
Cuts at: 2661 4141 2661
Size: 1480 4300
BstXI CCAn_nnnn'nTGG
Cuts at: 658 658
Size: 5780
BstYI r'GATC_y
Cuts at: 1 540 802 2805 3272 3295 3630 3876 4888
Size: 539 262 2003 467 23 335 246 1012
Cuts at: 4888 4974 4986 5332 1
Size: 86 12 346 449
Fragments arranged by size:
2003 1012 539 467 449 346 335 262
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 2356 2690 107
Size: 2249 334 3197
Fragments arranged by size:
3197 2249 334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3730 3911 4074 3730
Size: 181 163 5436
Fragments arranged by size:
5436 181 163
BtsI GCAGTG_nn'
Cuts at: 779 3023 779
Size: 2244 3536
DraI TTT'AAA
Cuts at: 1229 1926 2779 1229
Size: 697 853 4230
Fragments arranged by size:
4230 853 697
DrdI GACnn_nn'nnGTC
Cuts at: 3486 4344 3486
Size: 858 4922
EaeI y'GGCC_r
Cuts at: 2687 3365 3539 3930 3957 5302 2687
Size: 678 174 391 27 1345 3165
Fragments arranged by size:
3165 1345 678 391 174 27
EagI C'GGCC_G
Cuts at: 3365 3365
Size: 5780
EarI CTCTTCn'nnn_
Cuts at: 2276 2663 3803 4013 2276
Size: 387 1140 210 4043
Fragments arranged by size:
4043 1140 387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3988 4298 4444 5590 3988
Size: 310 146 1146 4178
Fragments arranged by size:
4178 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2792 2792
Size: 5780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 1328 1567 1882 2228 3605 4037 4784 5052
Size: 492 239 315 346 1377 432 747 268
Cuts at: 5052 836
Size: 1564
Fragments arranged by size:
1564 1377 747 492 432 346 315 268
239
EcoNI CCTnn'n_nnAGG
Cuts at: 1369 1456 1369
Size: 87 5693
EcoO109I rG'GnC_Cy
Cuts at: 2361 2919 2361
Size: 558 5222
EcoRI G'AATT_C
Cuts at: 1838 2784 1838
Size: 946 4834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 190 484 744 1858 3799 190
Size: 294 260 1114 1941 2171
Fragments arranged by size:
2171 1941 1114 294 260
FspI TGC'GCA
Cuts at: 3561 3561
Size: 5780
HaeII r_GCGC'y
Cuts at: 246 3214 3462 4484 5463 246
Size: 2968 248 1022 979 563
Fragments arranged by size:
2968 1022 979 563 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1438 1470 2137 2169 3622 3652 3654 3684 1438
Size: 32 667 32 1453 30 2 30 3534
Fragments arranged by size:
3534 1453 667 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1778 2819 1778
Size: 1041 4739
HindIII A'AGCT_T
Cuts at: 257 257
Size: 5780
HpaI GTT'AAC
Cuts at: 1778 1778
Size: 5780
KasI G'GCGC_C
Cuts at: 3210 3458 3210
Size: 248 5532
KpnI G_GTAC'C
Cuts at: 2800 2800
Size: 5780
MluI A'CGCG_T
Cuts at: 5213 5213
Size: 5780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 289 780 2107 4451 4635 289
Size: 491 1327 2344 184 1434
Fragments arranged by size:
2344 1434 1327 491 184
MscI TGG'CCA
Cuts at: 3541 3541
Size: 5780
MslI CAynn'nnrTG
Cuts at: 979 1022 3769 3896 979
Size: 43 2747 127 2863
Fragments arranged by size:
2863 2747 127 43
MspA1I CmG'CkG
Cuts at: 2891 3205 3565 4578 4823 2891
Size: 314 360 1013 245 3848
Fragments arranged by size:
3848 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3961 3961
Size: 5780
NarI GG'CG_CC
Cuts at: 3211 3459 3211
Size: 248 5532
NcoI C'CATG_G
Cuts at: 107 2356 107
Size: 2249 3531
NgoMIV G'CCGG_C
Cuts at: 3959 3959
Size: 5780
NspI r_CATG'y
Cuts at: 982 1892 2833 3333 4240 982
Size: 910 941 500 907 2522
Fragments arranged by size:
2522 941 910 907 500
PciI A'CATG_T
Cuts at: 978 1888 4236 978
Size: 910 2348 2522
Fragments arranged by size:
2522 2348 910
PflMI CCAn_nnn'nTGG
Cuts at: 608 753 608
Size: 145 5635
PfoI T'CCnGG_A
Cuts at: 1412 1412
Size: 5780
PmeI GTTT'AAAC
Cuts at: 2779 2779
Size: 5780
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 4919
Size: 32 5748
PpuMI rG'GwC_Cy
Cuts at: 2361 2361
Size: 5780
PsiI TTA'TAA
Cuts at: 413 1559 413
Size: 1146 4634
PspXI vC'TCGA_Gb
Cuts at: 2180 2180
Size: 5780
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 662 694 662
Size: 32 5748
PstI C_TGCA'G
Cuts at: 2827 3512 2827
Size: 685 5095
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5780
PvuII CAG'CTG
Cuts at: 3205 3565 3205
Size: 360 5420
RsrII CG'GwC_CG
Cuts at: 3975 3975
Size: 5780
SacI G_AGCT'C
Cuts at: 2794 2794
Size: 5780
SalI G'TCGA_C
Cuts at: 2817 2817
Size: 5780
SanDI GG'GwC_CC
Cuts at: 2361 2361
Size: 5780
SapI GCTCTTCn'nnn_
Cuts at: 2663 3803 4013 2663
Size: 1140 210 4430
Fragments arranged by size:
4430 1140 210
SbfI CC_TGCA'GG
Cuts at: 2827 2827
Size: 5780
ScaI AGT'ACT
Cuts at: 5297 5297
Size: 5780
SfcI C'TryA_G
Cuts at: 33 2823 3508 4501 4692 33
Size: 2790 685 993 191 1121
Fragments arranged by size:
2790 1121 993 685 191
SfoI GGC'GCC
Cuts at: 3212 3460 3212
Size: 248 5532
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5780
SmaI CCC'GGG
Cuts at: 2802 2802
Size: 5780
SmlI C'TyrA_G
Cuts at: 469 2180 2935 3850 4342 4604 469
Size: 1711 755 915 492 262 1645
Fragments arranged by size:
1711 1645 915 755 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5780
SphI G_CATG'C
Cuts at: 2833 3333 2833
Size: 500 5280
SspI AAT'ATT
Cuts at: 1757 1884 2649 1757
Size: 127 765 4888
Fragments arranged by size:
4888 765 127
StyI C'CwwG_G
Cuts at: 107 174 246 1370 1941 2356 2914 3268 107
Size: 67 72 1124 571 415 558 354 2619
Fragments arranged by size:
2619 1124 571 558 415 354 72 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3363 3496 3955 4164 3363
Size: 133 459 209 4979
Fragments arranged by size:
4979 459 209 133
TatI w'GTAC_w
Cuts at: 529 2560 5295 529
Size: 2031 2735 1014
Fragments arranged by size:
2735 2031 1014
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5297 5329 5297
Size: 32 5748
Tth111I GACn'n_nGTC
Cuts at: 3577 3577
Size: 5780
XbaI T'CTAG_A
Cuts at: 2811 2811
Size: 5780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1264 3275 1264
Size: 2011 3769
XhoI C'TCGA_G
Cuts at: 2180 2180
Size: 5780
XmaI C'CCGG_G
Cuts at: 2800 2800
Size: 5780
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PspOMI SacII
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI
ZraI