Restriction map for
pF1KA1457
(Circular) (Six-base) MAPSORT of: pF1KA1457.seq Check: 8215 from: 1 to: 7142
pF1KA1457 7142 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3701 3974 3701
Size: 273 6869
AccI GT'mk_AC
Cuts at: 2045 2372 3329 4180 2045
Size: 327 957 851 5007
Fragments arranged by size:
5007 957 851 327
Acc65I G'GTAC_C
Cuts at: 4158 4158
Size: 7142
AclI AA'CG_TT
Cuts at: 6982 6982
Size: 7142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3402 3688 3874 4967 5399 6146 6414 3402
Size: 286 186 1093 432 747 268 4130
Fragments arranged by size:
4130 1093 747 432 286 268 186
AflIII A'CryG_T
Cuts at: 479 1469 3362 4494 5598 6575 479
Size: 990 1893 1132 1104 977 1046
Fragments arranged by size:
1893 1132 1104 1046 990 977
AgeI A'CCGG_T
Cuts at: 5530 5530
Size: 7142
AhdI GACnn_n'nnGTC
Cuts at: 1902 1902
Size: 7142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1121 1950 1953 3922 1121
Size: 829 3 1969 4341
Fragments arranged by size:
4341 1969 829 3
AlwNI CAG_nnn'CTG
Cuts at: 1350 2930 3147 3516 4447 6014 1350
Size: 1580 217 369 931 1567 2478
Fragments arranged by size:
2478 1580 1567 931 369 217
ApaI G_GGCC'C
Cuts at: 620 3173 4001 4132 620
Size: 2553 828 131 3630
Fragments arranged by size:
3630 2553 828 131
ApaLI G'TGCA_C
Cuts at: 1492 3211 3700 5912 1492
Size: 1719 489 2212 2722
Fragments arranged by size:
2722 2212 1719 489
ApoI r'AATT_y
Cuts at: 424 4146 6495 6751 424
Size: 3722 2349 256 815
Fragments arranged by size:
3722 2349 815 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7142
AvaI C'yCGr_G
Cuts at: 1908 2640 4162 1908
Size: 732 1522 4888
Fragments arranged by size:
4888 1522 732
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3248 3281 3248
Size: 33 7109
BamHI G'GATC_C
Cuts at: 4167 4167
Size: 7142
BanI G'GyrC_C
Cuts at: 1279 1402 1420 1533 2024 2236 2285 2698 4158
Size: 123 18 113 491 212 49 413 1460
Cuts at: 4158 4572 4820 4855 1279
Size: 414 248 35 3566
Fragments arranged by size:
3566 1460 491 414 413 248 212 123
113 49 35 18
BanII G_rGCy'C
Cuts at: 620 777 917 959 968 989 1129 1210 1240
Size: 157 140 42 9 21 140 81 30
Cuts at: 1240 2192 3173 4001 4132 4156 5186 620
Size: 952 981 828 131 24 1030 2576
Fragments arranged by size:
2576 1030 981 952 828 157 140 140
131 81 42 30 24 21 9
BbeI G_GCGC'C
Cuts at: 1424 2702 4576 4824 1424
Size: 1278 1874 248 3742
Fragments arranged by size:
3742 1874 1278 248
BbsI GAAGACnn'nnnn_
Cuts at: 651 1256 2323 3408 651
Size: 605 1067 1085 4385
Fragments arranged by size:
4385 1085 1067 605
BbvCI CC'TCA_GC
Cuts at: 1582 2179 1582
Size: 597 6545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3390 3424 3390
Size: 34 7108
BciVI GTATCCnnnnn_n'
Cuts at: 2121 3249 5040 5801 7142 2121
Size: 1128 1791 761 1341 2121
Fragments arranged by size:
2121 1791 1341 1128 761
BclI T'GATC_A
Cuts at: 4662 4662
Size: 7142
BglI GCCn_nnn'nGGC
Cuts at: 266 1868 2622 2884 3844 3932 3996 266
Size: 1602 754 262 960 88 64 3412
Fragments arranged by size:
3412 1602 960 754 262 88 64
BglII A'GATC_T
Cuts at: 3767 4657 6694 3767
Size: 890 2037 4215
Fragments arranged by size:
4215 2037 890
BlpI GC'TnA_GC
Cuts at: 817 4254 817
Size: 3437 3705
Bme1580I G_kGCm'C
Cuts at: 554 620 1284 1496 1538 2241 2290 2719 3173
Size: 66 664 212 42 703 49 429 454
Cuts at: 3173 3215 3403 3704 4001 4132 4767 4860 5916
Size: 42 188 301 297 131 635 93 1056
Cuts at: 5916 554
Size: 1780
Fragments arranged by size:
1780 1056 703 664 635 454 429 301
297 212 188 131 93 66 49 42
42
BmgBI CAC'GTC
Cuts at: 480 2946 3495 3729 480
Size: 2466 549 234 3893
Fragments arranged by size:
3893 2466 549 234
BmrI ACTGGGnnnn_n'
Cuts at: 717 876 1668 2940 3203 4354 4435 4770 6914
Size: 159 792 1272 263 1151 81 335 2144
Cuts at: 6914 717
Size: 945
Fragments arranged by size:
2144 1272 1151 945 792 335 263 159
81
BmtI G_CTAG'C
Cuts at: 3446 3446
Size: 7142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 2211 5509 444
Size: 1767 3298 2077
Fragments arranged by size:
3298 2077 1767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 485 862 1287 1989 2032 2434 2566 2650
Size: 229 377 425 702 43 402 132 84
Cuts at: 2650 2973 4129 256
Size: 323 1156 3269
Fragments arranged by size:
3269 1156 702 425 402 377 323 229
132 84 43
Bpu10I CC'TnA_GC
Cuts at: 1582 2029 2179 2534 1582
Size: 447 150 355 6190
Fragments arranged by size:
6190 447 355 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 2331 4318 5197 5689 5987 991
Size: 1340 1987 879 492 298 2146
Fragments arranged by size:
2146 1987 1340 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1578 2509 2955 3350 6846 1578
Size: 931 446 395 3496 1874
Fragments arranged by size:
3496 1874 931 446 395
BsaAI yAC'GTr
Cuts at: 1377 3075 3537 4495 1377
Size: 1698 462 958 4024
Fragments arranged by size:
4024 1698 958 462
BsaBI GATnn'nnATC
Cuts at: 6 2761 3772 4656 6693 6
Size: 2755 1011 884 2037 455
Fragments arranged by size:
2755 2037 1011 884 455
BsaHI Gr'CG_yC
Cuts at: 1421 1559 2699 2876 4573 4821 5523 7019 1421
Size: 138 1140 177 1697 248 702 1496 1544
Fragments arranged by size:
1697 1544 1496 1140 702 248 177 138
BsaWI w'CCGG_w
Cuts at: 748 3104 4331 4852 5530 5804 5951 6362 748
Size: 2356 1227 521 678 274 147 411 1528
Fragments arranged by size:
2356 1528 1227 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1420 1450 2935 2965 1420
Size: 30 1485 30 5597
Fragments arranged by size:
5597 1485 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 379 655 916 1656 2115 2697 166
Size: 213 276 261 740 459 582 4611
Fragments arranged by size:
4611 740 582 459 276 261 213
BseYI C'CCAG_C
Cuts at: 167 316 608 967 1432 1501 2292 2328 2484
Size: 149 292 359 465 69 791 36 156
Cuts at: 2484 3224 3587 3986 3991 4000 4082 4567 5325
Size: 740 363 399 5 9 82 485 758
Cuts at: 5325 5902 6398 167
Size: 577 496 911
Fragments arranged by size:
911 791 758 740 577 496 485 465
399 363 359 292 156 149 82 69
36 9 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1378 2542 3673 1340
Size: 38 1164 1131 4809
Fragments arranged by size:
4809 1164 1131 38
BsiEI CG_ry'CG
Cuts at: 104 247 1045 2356 4076 4684 4730 5938 104
Size: 143 798 1311 1720 608 46 1208 1308
Fragments arranged by size:
1720 1311 1308 1208 798 608 143 46
BsiHKAI G_wGCw'C
Cuts at: 917 1129 1210 1496 2666 3215 3704 4156 4934
Size: 212 81 286 1170 549 489 452 778
Cuts at: 4934 5124 5916 917
Size: 190 792 2143
Fragments arranged by size:
2143 1170 792 778 549 489 452 286
212 190 81
BsmI GAATG_Cn'
Cuts at: 381 1929 381
Size: 1548 5594
BsmBI CGTCTCn'nnnn_
Cuts at: 939 1043 939
Size: 104 7038
Bsp1286I G_dGCh'C
Cuts at: 554 620 777 917 959 968 989 1129 1210
Size: 66 157 140 42 9 21 140 81
Cuts at: 1210 1240 1284 1496 1538 2192 2241 2290 2666
Size: 30 44 212 42 654 49 49 376
Cuts at: 2666 2719 3173 3215 3403 3704 4001 4132 4156
Size: 53 454 42 188 301 297 131 24
Cuts at: 4156 4767 4860 4934 5124 5186 5916 554
Size: 611 93 74 190 62 730 1780
Fragments arranged by size:
1780 730 654 611 454 376 301 297
212 190 188 157 140 140 131 93
81 74 66 62 53 49 49 44
42 42 42 30 24 21 9
BspEI T'CCGG_A
Cuts at: 6362 6362
Size: 7142
BspHI T'CATG_A
Cuts at: 2972 3218 6318 2972
Size: 246 3100 3796
Fragments arranged by size:
3796 3100 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 645 1758 1907 2237 3146 3701 3974 4192 4406
Size: 1113 149 330 909 555 273 218 214
Cuts at: 4406 4708 5089 645
Size: 302 381 2698
Fragments arranged by size:
2698 1113 909 555 381 330 302 273
218 214 149
BsrBI CCG'CTC
Cuts at: 50 329 3880 4017 5434 5488 6972 7137 50
Size: 279 3551 137 1417 54 1484 165 55
Fragments arranged by size:
3551 1484 1417 279 165 137 55 54
BsrDI GCAATG_nn'
Cuts at: 984 5054 984
Size: 4070 3072
BsrFI r'CCGG_y
Cuts at: 242 779 1836 2355 3545 3782 4007 4075 5140
Size: 537 1057 519 1190 237 225 68 1065
Cuts at: 5140 5321 5530 242
Size: 181 209 1854
Fragments arranged by size:
1854 1190 1065 1057 537 519 237 225
209 181 68
BsrGI T'GTAC_A
Cuts at: 173 3737 173
Size: 3564 3578
BssSI C'ACGA_G
Cuts at: 1153 4012 5413 5771 1153
Size: 2859 1401 358 2524
Fragments arranged by size:
2859 2524 1401 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1813 4519 1813
Size: 2706 4436
BstBI TT'CG_AA
Cuts at: 5503 5503
Size: 7142
BstEII G'GTnAC_C
Cuts at: 3183 3264 3183
Size: 81 7061
BstXI CCAn_nnnn'nTGG
Cuts at: 949 1509 1530 2223 2508 2695 2997 3729 3900
Size: 560 21 693 285 187 302 732 171
Cuts at: 3900 4036 949
Size: 136 4055
Fragments arranged by size:
4055 732 693 560 302 285 187 171
136 21
BstYI r'GATC_y
Cuts at: 1 230 1370 3767 4167 4634 4657 4992 5238
Size: 229 1140 2397 400 467 23 335 246
Cuts at: 5238 6250 6336 6348 6694 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2397 1140 1012 467 449 400 346 335
246 229 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3234 3234
Size: 7142
BtgI C'CryG_G
Cuts at: 146 2390 2574 2894 3488 3603 4124 146
Size: 2244 184 320 594 115 521 3164
Fragments arranged by size:
3164 2244 594 521 320 184 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 147 1446 1463 2748 2796 5092 5273 5436 147
Size: 1299 17 1285 48 2296 181 163 1853
Fragments arranged by size:
2296 1853 1299 1285 181 163 48 17
BtsI GCAGTG_nn'
Cuts at: 1932 1983 2717 3814 4385 1932
Size: 51 734 1097 571 4689
Fragments arranged by size:
4689 1097 734 571 51
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 128 163 2927 2962 3258 3293 128
Size: 35 2764 35 296 35 3977
Fragments arranged by size:
3977 2764 296 35 35 35
DraI TTT'AAA
Cuts at: 4141 4141
Size: 7142
DraIII CAC_nnn'GTG
Cuts at: 291 1202 291
Size: 911 6231
DrdI GACnn_nn'nnGTC
Cuts at: 4848 5706 4848
Size: 858 6284
EaeI y'GGCC_r
Cuts at: 244 1658 2353 3095 3890 3933 4073 4101 4727
Size: 1414 695 742 795 43 140 28 626
Cuts at: 4727 4901 5292 5319 6664 244
Size: 174 391 27 1345 722
Fragments arranged by size:
1414 1345 795 742 722 695 626 391
174 140 43 28 27
EagI C'GGCC_G
Cuts at: 244 2353 4073 4727 244
Size: 2109 1720 654 2659
Fragments arranged by size:
2659 2109 1720 654
EarI CTCTTCn'nnn_
Cuts at: 184 569 2278 2284 2386 2920 5165 5375 184
Size: 385 1709 6 102 534 2245 210 1951
Fragments arranged by size:
2245 1951 1709 534 385 210 102 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1516 2731 4048 5350 5660 5806 6952 1516
Size: 1215 1317 1302 310 146 1146 1706
Fragments arranged by size:
1706 1317 1302 1215 1146 310 146
EcoICRI GAG'CTC
Cuts at: 915 1127 1208 4154 915
Size: 212 81 2946 3903
Fragments arranged by size:
3903 2946 212 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 485 862 1287 1989 2032 2434 2566 2650
Size: 229 377 425 702 43 402 132 84
Cuts at: 2650 2973 3402 3688 3874 4129 4967 5399 6146
Size: 323 429 286 186 255 838 432 747
Cuts at: 6146 6414 256
Size: 268 984
Fragments arranged by size:
984 838 747 702 432 429 425 402
377 323 286 268 255 229 186 132
84 43
EcoNI CCTnn'n_nnAGG
Cuts at: 340 340
Size: 7142
EcoO109I rG'GnC_Cy
Cuts at: 579 616 617 1158 1428 2073 3170 3513 4129
Size: 37 1 541 270 645 1097 343 616
Cuts at: 4129 4281 579
Size: 152 3440
Fragments arranged by size:
3440 1097 645 616 541 343 270 152
37 1
EcoRI G'AATT_C
Cuts at: 4146 4146
Size: 7142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 675 3678 3936 5161 675
Size: 3003 258 1225 2656
Fragments arranged by size:
3003 2656 1225 258
FseI GG_CCGG'CC
Cuts at: 2359 3549 3786 2359
Size: 1190 237 5715
Fragments arranged by size:
5715 1190 237
FspI TGC'GCA
Cuts at: 675 1151 3910 4923 675
Size: 476 2759 1013 2894
Fragments arranged by size:
2894 2759 1013 476
FspAI rTGC'GCAy
Cuts at: 675 1151 675
Size: 476 6666
HaeII r_GCGC'y
Cuts at: 1424 1508 2436 2702 3746 3907 4576 4824 5846
Size: 84 928 266 1044 161 669 248 1022
Cuts at: 5846 6825 1424
Size: 979 1741
Fragments arranged by size:
1741 1044 1022 979 928 669 266 248
161 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 931 963 1111 1143 1147 1179 1391 1420 1423
Size: 32 148 32 4 32 212 29 3
Cuts at: 1423 1452 1888 1920 2482 2514 2935 2967 4984
Size: 29 436 32 562 32 421 32 2017
Cuts at: 4984 5014 5016 5046 931
Size: 30 2 30 3027
Fragments arranged by size:
3027 2017 562 436 421 212 148 32
32 32 32 32 32 30 30 29
29 4 3 2
HincII GTy'rAC
Cuts at: 599 2046 2939 4181 599
Size: 1447 893 1242 3560
Fragments arranged by size:
3560 1447 1242 893
KasI G'GCGC_C
Cuts at: 1420 2698 4572 4820 1420
Size: 1278 1874 248 3742
Fragments arranged by size:
3742 1874 1278 248
KpnI G_GTAC'C
Cuts at: 4162 4162
Size: 7142
MluI A'CGCG_T
Cuts at: 6575 6575
Size: 7142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1601 2636 5813 5997 1190
Size: 411 1035 3177 184 2335
Fragments arranged by size:
3177 2335 1035 411 184
MscI TGG'CCA
Cuts at: 1660 4903 1660
Size: 3243 3899
MslI CAynn'nnrTG
Cuts at: 1483 1489 1918 3385 3535 3898 4066 5131 5258
Size: 6 429 1467 150 363 168 1065 127
Cuts at: 5258 1483
Size: 3367
Fragments arranged by size:
3367 1467 1065 429 363 168 150 127
6
MspA1I CmG'CkG
Cuts at: 171 221 320 513 612 1317 1350 2292 2349
Size: 50 99 193 99 705 33 942 57
Cuts at: 2349 2392 2421 2484 3567 3645 3852 3995 4047
Size: 43 29 63 1083 78 207 143 52
Cuts at: 4047 4126 4253 4567 4927 5940 6185 171
Size: 79 127 314 360 1013 245 1128
Fragments arranged by size:
1128 1083 1013 942 705 360 314 245
207 193 143 127 99 99 79 78
63 57 52 50 43 33 29
NaeI GCC'GGC
Cuts at: 2357 3547 3784 4077 5323 2357
Size: 1190 237 293 1246 4176
Fragments arranged by size:
4176 1246 1190 293 237
NarI GG'CG_CC
Cuts at: 1421 2699 4573 4821 1421
Size: 1278 1874 248 3742
Fragments arranged by size:
3742 1874 1278 248
NcoI C'CATG_G
Cuts at: 3603 3603
Size: 7142
NdeI CA'TA_TG
Cuts at: 207 207
Size: 7142
NgoMIV G'CCGG_C
Cuts at: 2355 3545 3782 4075 5321 2355
Size: 1190 237 293 1246 4176
Fragments arranged by size:
4176 1246 1190 293 237
NheI G'CTAG_C
Cuts at: 3442 3442
Size: 7142
NspI r_CATG'y
Cuts at: 309 717 1887 3558 4195 4695 5602 309
Size: 408 1170 1671 637 500 907 1849
Fragments arranged by size:
1849 1671 1170 907 637 500 408
PasI CC'CwG_GG
Cuts at: 1509 2241 1509
Size: 732 6410
PciI A'CATG_T
Cuts at: 5598 5598
Size: 7142
PflMI CCAn_nnn'nTGG
Cuts at: 1350 2930 3609 1350
Size: 1580 679 4883
Fragments arranged by size:
4883 1580 679
PfoI T'CCnGG_A
Cuts at: 233 833 1409 2543 2588 3955 233
Size: 600 576 1134 45 1367 3420
Fragments arranged by size:
3420 1367 1134 600 576 45
PmeI GTTT'AAAC
Cuts at: 4141 4141
Size: 7142
PmlI CAC'GTG
Cuts at: 1377 3075 1377
Size: 1698 5444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1165 1197 6281 6313 1165
Size: 32 5084 32 1994
Fragments arranged by size:
5084 1994 32 32
PpuMI rG'GwC_Cy
Cuts at: 579 1158 1428 2073 3513 579
Size: 579 270 645 1440 4208
Fragments arranged by size:
4208 1440 645 579 270
PsiI TTA'TAA
Cuts at: 115 452 115
Size: 337 6805
PspOMI G'GGCC_C
Cuts at: 616 3169 3997 4128 616
Size: 2553 828 131 3630
Fragments arranged by size:
3630 2553 828 131
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 232 264 232
Size: 32 7110
PstI C_TGCA'G
Cuts at: 3803 4189 4874 3803
Size: 386 685 6071
Fragments arranged by size:
6071 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7142
PvuII CAG'CTG
Cuts at: 171 320 513 1317 2349 3852 4567 4927 171
Size: 149 193 804 1032 1503 715 360 2386
Fragments arranged by size:
2386 1503 1032 804 715 360 193 149
RsrII CG'GwC_CG
Cuts at: 5337 5337
Size: 7142
SacI G_AGCT'C
Cuts at: 917 1129 1210 4156 917
Size: 212 81 2946 3903
Fragments arranged by size:
3903 2946 212 81
SacII CC_GC'GG
Cuts at: 2393 4127 2393
Size: 1734 5408
SalI G'TCGA_C
Cuts at: 2044 4179 2044
Size: 2135 5007
SanDI GG'GwC_CC
Cuts at: 1428 1428
Size: 7142
SapI GCTCTTCn'nnn_
Cuts at: 184 5165 5375 184
Size: 4981 210 1951
Fragments arranged by size:
4981 1951 210
SbfI CC_TGCA'GG
Cuts at: 4189 4189
Size: 7142
ScaI AGT'ACT
Cuts at: 6659 6659
Size: 7142
SexAI A'CCwGG_T
Cuts at: 397 397
Size: 7142
SfcI C'TryA_G
Cuts at: 33 1590 1824 3799 4185 4870 5863 6054 33
Size: 1557 234 1975 386 685 993 191 1121
Fragments arranged by size:
1975 1557 1121 993 685 386 234 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3932 3932
Size: 7142
SfoI GGC'GCC
Cuts at: 1422 2700 4574 4822 1422
Size: 1278 1874 248 3742
Fragments arranged by size:
3742 1874 1278 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7142
SmaI CCC'GGG
Cuts at: 1910 2642 4164 1910
Size: 732 1522 4888
Fragments arranged by size:
4888 1522 732
SmlI C'TyrA_G
Cuts at: 1006 2310 4297 5212 5704 5966 1006
Size: 1304 1987 915 492 262 2182
Fragments arranged by size:
2182 1987 1304 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7142
SphI G_CATG'C
Cuts at: 309 717 1887 4195 4695 309
Size: 408 1170 2308 500 2756
Fragments arranged by size:
2756 2308 1170 500 408
SrfI GCCC'GGGC
Cuts at: 2642 2642
Size: 7142
StuI AGG'CCT
Cuts at: 787 2149 787
Size: 1362 5780
StyI C'CwwG_G
Cuts at: 341 1187 3043 3395 3470 3603 3722 4133 4276
Size: 846 1856 352 75 133 119 411 143
Cuts at: 4276 4630 341
Size: 354 2853
Fragments arranged by size:
2853 1856 846 411 354 352 143 133
119 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1031 3313 4725 4858 5317 5526 1031
Size: 2282 1412 133 459 209 2647
Fragments arranged by size:
2647 2282 1412 459 209 133
TatI w'GTAC_w
Cuts at: 173 278 644 2864 3737 3857 6657 173
Size: 105 366 2220 873 120 2800 658
Fragments arranged by size:
2800 2220 873 658 366 120 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6659 6691 6659
Size: 32 7110
Tth111I GACn'n_nGTC
Cuts at: 535 1480 1789 2947 3181 4939 535
Size: 945 309 1158 234 1758 2738
Fragments arranged by size:
2738 1758 1158 945 309 234
XbaI T'CTAG_A
Cuts at: 4173 4173
Size: 7142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1218 2121 3050 3726 4637 1218
Size: 903 929 676 911 3723
Fragments arranged by size:
3723 929 911 903 676
XmaI C'CCGG_G
Cuts at: 1908 2640 4162 1908
Size: 732 1522 4888
Fragments arranged by size:
4888 1522 732
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BfrBI
BsiWI BssHII BstZ17I ClaI EcoRV HindIII HpaI MfeI
NotI NruI NsiI PacI PshAI PspXI SgrAI SnaBI
SspI SwaI XhoI XmnI ZraI