Restriction map for
pF1KA1462
(Circular) (Six-base) MAPSORT of: pF1KA1462.seq Check: 5979 from: 1 to: 7190
pF1KA1462 7190 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1768 1768
Size: 7190
AatII G_ACGT'C
Cuts at: 286 286
Size: 7190
AccI GT'mk_AC
Cuts at: 4228 4228
Size: 7190
Acc65I G'GTAC_C
Cuts at: 4206 4206
Size: 7190
AclI AA'CG_TT
Cuts at: 7030 7030
Size: 7190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1253 3726 3918 5015 5447 6194 6462 1253
Size: 2473 192 1097 432 747 268 1981
Fragments arranged by size:
2473 1981 1097 747 432 268 192
AflIII A'CryG_T
Cuts at: 300 762 4542 5646 6623 300
Size: 462 3780 1104 977 867
Fragments arranged by size:
3780 1104 977 867 462
AgeI A'CCGG_T
Cuts at: 2159 2320 2574 5578 2159
Size: 161 254 3004 3771
Fragments arranged by size:
3771 3004 254 161
AhdI GACnn_n'nnGTC
Cuts at: 3252 3252
Size: 7190
AleI CACnn'nnGTG
Cuts at: 2882 2882
Size: 7190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2958 3477 2958
Size: 519 6671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3688 3720 3688
Size: 32 7158
AlwNI CAG_nnn'CTG
Cuts at: 234 640 1105 1977 2281 3667 3793 3874 4495
Size: 406 465 872 304 1386 126 81 621
Cuts at: 4495 6062 234
Size: 1567 1362
Fragments arranged by size:
1567 1386 1362 872 621 465 406 304
126 81
ApaI G_GGCC'C
Cuts at: 2533 2533
Size: 7190
ApaLI G'TGCA_C
Cuts at: 2933 5960 2933
Size: 3027 4163
ApoI r'AATT_y
Cuts at: 771 842 909 927 4194 6543 6799 771
Size: 71 67 18 3267 2349 256 1162
Fragments arranged by size:
3267 2349 1162 256 71 67 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 7190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7190
AvaI C'yCGr_G
Cuts at: 820 1621 2901 4002 4210 820
Size: 801 1280 1101 208 3800
Fragments arranged by size:
3800 1280 1101 801 208
AvrII C'CTAG_G
Cuts at: 3094 3859 3094
Size: 765 6425
BamHI G'GATC_C
Cuts at: 3371 4215 3371
Size: 844 6346
BanI G'GyrC_C
Cuts at: 194 1191 1522 2156 2427 2799 3286 4145 4206
Size: 997 331 634 271 372 487 859 61
Cuts at: 4206 4620 4868 4903 194
Size: 414 248 35 2481
Fragments arranged by size:
2481 997 859 634 487 414 372 331
271 248 61 35
BanII G_rGCy'C
Cuts at: 353 1091 1309 1328 1957 2204 2487 2525 2533
Size: 738 218 19 629 247 283 38 8
Cuts at: 2533 2633 2812 3104 3203 3236 3266 3449 3577
Size: 100 179 292 99 33 30 183 128
Cuts at: 3577 3615 3686 3907 4001 4204 5234 353
Size: 38 71 221 94 203 1030 2309
Fragments arranged by size:
2309 1030 738 629 292 283 247 221
218 203 183 179 128 100 99 94
71 38 38 33 30 19 8
BbeI G_GCGC'C
Cuts at: 198 4624 4872 198
Size: 4426 248 2516
Fragments arranged by size:
4426 2516 248
BbsI GAAGACnn'nnnn_
Cuts at: 128 1190 1445 1728 1982 2084 3035 128
Size: 1062 255 283 254 102 951 4283
Fragments arranged by size:
4283 1062 951 283 255 254 102
BbvCI CC'TCA_GC
Cuts at: 408 2374 408
Size: 1966 5224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3439 3473 3439
Size: 34 7156
BciVI GTATCCnnnnn_n'
Cuts at: 136 899 5088 5849 7190 136
Size: 763 4189 761 1341 136
Fragments arranged by size:
4189 1341 763 761 136
BclI T'GATC_A
Cuts at: 1929 2325 2600 4710 1929
Size: 396 275 2110 4409
Fragments arranged by size:
4409 2110 396 275
BglI GCCn_nnn'nGGC
Cuts at: 1323 3610 1323
Size: 2287 4903
BglII A'GATC_T
Cuts at: 2058 4705 6742 2058
Size: 2647 2037 2506
Fragments arranged by size:
2647 2506 2037
BlpI GC'TnA_GC
Cuts at: 948 1418 1981 2946 3443 4302 948
Size: 470 563 965 497 859 3836
Fragments arranged by size:
3836 965 859 563 497 470
Bme1580I G_kGCm'C
Cuts at: 1196 1715 2159 2349 2533 2937 4041 4150 4815
Size: 519 444 190 184 404 1104 109 665
Cuts at: 4815 4908 5964 1196
Size: 93 1056 2422
Fragments arranged by size:
2422 1104 1056 665 519 444 404 190
184 109 93
BmrI ACTGGGnnnn_n'
Cuts at: 221 1295 1805 1858 1916 4402 4483 4818 6962
Size: 1074 510 53 58 2486 81 335 2144
Cuts at: 6962 221
Size: 449
Fragments arranged by size:
2486 2144 1074 510 449 335 81 58
53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1976 3921 5557 1976
Size: 1945 1636 3609
Fragments arranged by size:
3609 1945 1636
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1103 1455 2023 2617 2874 3004 3227 4068
Size: 574 352 568 594 257 130 223 841
Cuts at: 4068 529
Size: 3651
Fragments arranged by size:
3651 841 594 574 568 352 257 223
130
Bpu10I CC'TnA_GC
Cuts at: 408 2374 2498 3674 3680 3928 408
Size: 1966 124 1176 6 248 3670
Fragments arranged by size:
3670 1966 1176 248 124 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1472 2116 2385 4366 5245 5737 6035 970
Size: 502 644 269 1981 879 492 298 2125
Fragments arranged by size:
2125 1981 879 644 502 492 298 269
BsaI GGTCTCn'nnnn_
Cuts at: 152 1652 6894 152
Size: 1500 5242 448
Fragments arranged by size:
5242 1500 448
BsaAI yAC'GTr
Cuts at: 552 1128 2884 4543 552
Size: 576 1756 1659 3199
Fragments arranged by size:
3199 1756 1659 576
BsaBI GATnn'nnATC
Cuts at: 6 4704 6741 6
Size: 4698 2037 455
Fragments arranged by size:
4698 2037 455
BsaHI Gr'CG_yC
Cuts at: 195 283 1067 2434 3959 4621 4869 5571 7067
Size: 88 784 1367 1525 662 248 702 1496
Cuts at: 7067 195
Size: 318
Fragments arranged by size:
1525 1496 1367 784 702 662 318 248
88
BsaWI w'CCGG_w
Cuts at: 452 2159 2320 2574 3416 4379 4900 5578 5852
Size: 1707 161 254 842 963 521 678 274
Cuts at: 5852 5999 6410 452
Size: 147 411 1232
Fragments arranged by size:
1707 1232 963 842 678 521 411 274
254 161 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 712 742 749 779 3688 3718 3998
Size: 30 309 30 7 30 2909 30 280
Cuts at: 3998 4028 4066 4096 373
Size: 30 38 30 3467
Fragments arranged by size:
3467 2909 309 280 38 30 30 30
30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1040 2212 2701 2875 3605 1040
Size: 1172 489 174 730 4625
Fragments arranged by size:
4625 1172 730 489 174
BseYI C'CCAG_C
Cuts at: 165 1246 2235 3501 3685 4615 5373 5950 6446
Size: 1081 989 1266 184 930 758 577 496
Cuts at: 6446 165
Size: 909
Fragments arranged by size:
1266 1081 989 930 909 758 577 496
184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2987 3447 2273
Size: 714 460 6016
Fragments arranged by size:
6016 714 460
BsiEI CG_ry'CG
Cuts at: 104 1238 4732 4778 5986 104
Size: 1134 3494 46 1208 1308
Fragments arranged by size:
3494 1308 1208 1134 46
BsiHKAI G_wGCw'C
Cuts at: 361 500 1091 1759 2633 2724 2937 3632 3907
Size: 139 591 668 874 91 213 695 275
Cuts at: 3907 4204 4982 5172 5964 361
Size: 297 778 190 792 1587
Fragments arranged by size:
1587 874 792 778 695 668 591 297
275 213 190 139 91
BsmI GAATG_Cn'
Cuts at: 1514 2136 3945 1514
Size: 622 1809 4759
Fragments arranged by size:
4759 1809 622
Bsp1286I G_dGCh'C
Cuts at: 353 361 500 1091 1196 1309 1328 1715 1759
Size: 8 139 591 105 113 19 387 44
Cuts at: 1759 1957 2159 2204 2349 2487 2525 2533 2633
Size: 198 202 45 145 138 38 8 100
Cuts at: 2633 2724 2812 2937 3104 3203 3236 3266 3449
Size: 91 88 125 167 99 33 30 183
Cuts at: 3449 3577 3615 3632 3686 3907 4001 4041 4150
Size: 128 38 17 54 221 94 40 109
Cuts at: 4150 4204 4815 4908 4982 5172 5234 5964 353
Size: 54 611 93 74 190 62 730 1579
Fragments arranged by size:
1579 730 611 591 387 221 202 198
190 183 167 145 139 138 128 125
113 109 105 100 99 94 93 91
88 74 62 54 54 45 44 40
38 38 33 30 19 17 8 8
BspEI T'CCGG_A
Cuts at: 3416 6410 3416
Size: 2994 4196
BspHI T'CATG_A
Cuts at: 6366 6366
Size: 7190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1768 3004 4240 4454 4756 5137 1768
Size: 1236 1236 214 302 381 3821
Fragments arranged by size:
3821 1236 1236 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1030 3424 5482 5536 7020 7185 50
Size: 980 2394 2058 54 1484 165 55
Fragments arranged by size:
2394 2058 1484 980 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5102 5102
Size: 7190
BsrFI r'CCGG_y
Cuts at: 1494 2159 2320 2574 3507 5188 5369 5578 1494
Size: 665 161 254 933 1681 181 209 3106
Fragments arranged by size:
3106 1681 933 665 254 209 181 161
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 7190
BssSI C'ACGA_G
Cuts at: 218 921 2961 5461 5819 218
Size: 703 2040 2500 358 1589
Fragments arranged by size:
2500 2040 1589 703 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4567 4567
Size: 7190
BstBI TT'CG_AA
Cuts at: 3710 5551 3710
Size: 1841 5349
BstXI CCAn_nnnn'nTGG
Cuts at: 522 2001 2479 2888 3693 522
Size: 1479 478 409 805 4019
Fragments arranged by size:
4019 1479 805 478 409
BstYI r'GATC_y
Cuts at: 1 2058 3371 4215 4682 4705 5040 5286 6298
Size: 2057 1313 844 467 23 335 246 1012
Cuts at: 6298 6384 6396 6742 1
Size: 86 12 346 449
Fragments arranged by size:
2057 1313 1012 844 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1319 3617 1319
Size: 2298 4892
BtgI C'CryG_G
Cuts at: 287 342 565 1393 2417 2472 2525 2751 287
Size: 55 223 828 1024 55 53 226 4726
Fragments arranged by size:
4726 1024 828 226 223 55 55 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5140 5321 5484 5140
Size: 181 163 6846
Fragments arranged by size:
6846 181 163
BtsI GCAGTG_nn'
Cuts at: 799 1044 1349 1481 2688 2724 3067 4032 4433
Size: 245 305 132 1207 36 343 965 401
Cuts at: 4433 799
Size: 3556
Fragments arranged by size:
3556 1207 965 401 343 305 245 132
36
DraI TTT'AAA
Cuts at: 1410 4189 1410
Size: 2779 4411
DrdI GACnn_nn'nnGTC
Cuts at: 1072 2412 3039 3649 4896 5754 1072
Size: 1340 627 610 1247 858 2508
Fragments arranged by size:
2508 1340 1247 858 627 610
EaeI y'GGCC_r
Cuts at: 1583 2149 2874 4775 4949 5340 5367 6712 1583
Size: 566 725 1901 174 391 27 1345 2061
Fragments arranged by size:
2061 1901 1345 725 566 391 174 27
EagI C'GGCC_G
Cuts at: 4775 4775
Size: 7190
EarI CTCTTCn'nnn_
Cuts at: 453 931 2633 2823 3479 4085 4117 5213 5423
Size: 478 1702 190 656 606 32 1096 210
Cuts at: 5423 453
Size: 2220
Fragments arranged by size:
2220 1702 1096 656 606 478 210 190
32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1124 2713 5398 5708 5854 7000 1124
Size: 1589 2685 310 146 1146 1314
Fragments arranged by size:
2685 1589 1314 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1089 2631 3905 4202 1089
Size: 1542 1274 297 4077
Fragments arranged by size:
4077 1542 1274 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 1103 1253 1455 2023 2617 2874 3004 3227
Size: 574 150 202 568 594 257 130 223
Cuts at: 3227 3726 3918 4068 5015 5447 6194 6462 529
Size: 499 192 150 947 432 747 268 1257
Fragments arranged by size:
1257 947 747 594 574 568 499 432
268 257 223 202 192 150 150 130
EcoNI CCTnn'n_nnAGG
Cuts at: 1268 1268
Size: 7190
EcoO109I rG'GnC_Cy
Cuts at: 231 637 980 1273 2013 2369 2530 4329 231
Size: 406 343 293 740 356 161 1799 3092
Fragments arranged by size:
3092 1799 740 406 356 343 293 161
EcoRI G'AATT_C
Cuts at: 771 927 4194 771
Size: 156 3267 3767
Fragments arranged by size:
3767 3267 156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5209 5209
Size: 7190
FspI TGC'GCA
Cuts at: 903 4971 903
Size: 4068 3122
HaeII r_GCGC'y
Cuts at: 198 4624 4872 5894 6873 198
Size: 4426 248 1022 979 515
Fragments arranged by size:
4426 1022 979 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 578 1444 1476 1562 1594 2827 2859 3238
Size: 32 866 32 86 32 1233 32 379
Cuts at: 3238 3270 3478 3510 4066 4098 4116 4148 5032
Size: 32 208 32 556 32 18 32 884
Cuts at: 5032 5062 5064 5094 546
Size: 30 2 30 2642
Fragments arranged by size:
2642 1233 884 866 556 379 208 86
32 32 32 32 32 32 32 32
30 30 18 2
HincII GTy'rAC
Cuts at: 1222 4229 1222
Size: 3007 4183
HindIII A'AGCT_T
Cuts at: 1447 1447
Size: 7190
KasI G'GCGC_C
Cuts at: 194 4620 4868 194
Size: 4426 248 2516
Fragments arranged by size:
4426 2516 248
KpnI G_GTAC'C
Cuts at: 4210 4210
Size: 7190
MluI A'CGCG_T
Cuts at: 6623 6623
Size: 7190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 555 1258 5861 6045 555
Size: 703 4603 184 1700
Fragments arranged by size:
4603 1700 703 184
MscI TGG'CCA
Cuts at: 2151 2876 4951 2151
Size: 725 2075 4390
Fragments arranged by size:
4390 2075 725
MslI CAynn'nnrTG
Cuts at: 251 305 1201 2882 3385 3635 5179 5306 251
Size: 54 896 1681 503 250 1544 127 2135
Fragments arranged by size:
2135 1681 1544 896 503 250 127 54
MspA1I CmG'CkG
Cuts at: 289 1256 1664 2217 2753 3088 4301 4615 4975
Size: 967 408 553 536 335 1213 314 360
Cuts at: 4975 5988 6233 289
Size: 1013 245 1246
Fragments arranged by size:
1246 1213 1013 967 553 536 408 360
335 314 245
NaeI GCC'GGC
Cuts at: 1496 5371 1496
Size: 3875 3315
NarI GG'CG_CC
Cuts at: 195 4621 4869 195
Size: 4426 248 2516
Fragments arranged by size:
4426 2516 248
NcoI C'CATG_G
Cuts at: 565 565
Size: 7190
NgoMIV G'CCGG_C
Cuts at: 1494 5369 1494
Size: 3875 3315
NspI r_CATG'y
Cuts at: 304 595 766 1989 4243 4743 5650 304
Size: 291 171 1223 2254 500 907 1844
Fragments arranged by size:
2254 1844 1223 907 500 291 171
PasI CC'CwG_GG
Cuts at: 1425 2491 2591 3271 3521 1425
Size: 1066 100 680 250 5094
Fragments arranged by size:
5094 1066 680 250 100
PciI A'CATG_T
Cuts at: 300 762 5646 300
Size: 462 4884 1844
Fragments arranged by size:
4884 1844 462
PflMI CCAn_nnn'nTGG
Cuts at: 1062 4047 1062
Size: 2985 4205
PfoI T'CCnGG_A
Cuts at: 2633 2633
Size: 7190
PmeI GTTT'AAAC
Cuts at: 4189 4189
Size: 7190
PmlI CAC'GTG
Cuts at: 552 2884 552
Size: 2332 4858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3688 3720 6329 6361 3688
Size: 32 2609 32 4517
Fragments arranged by size:
4517 2609 32 32
PpuMI rG'GwC_Cy
Cuts at: 637 2369 637
Size: 1732 5458
PspOMI G'GGCC_C
Cuts at: 2529 2529
Size: 7190
PstI C_TGCA'G
Cuts at: 239 2012 2731 2870 4237 4922 239
Size: 1773 719 139 1367 685 2507
Fragments arranged by size:
2507 1773 1367 719 685 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7190
PvuII CAG'CTG
Cuts at: 2217 3088 4615 4975 2217
Size: 871 1527 360 4432
Fragments arranged by size:
4432 1527 871 360
RsrII CG'GwC_CG
Cuts at: 5385 5385
Size: 7190
SacI G_AGCT'C
Cuts at: 1091 2633 3907 4204 1091
Size: 1542 1274 297 4077
Fragments arranged by size:
4077 1542 1274 297
SacII CC_GC'GG
Cuts at: 290 2754 290
Size: 2464 4726
SalI G'TCGA_C
Cuts at: 4227 4227
Size: 7190
SapI GCTCTTCn'nnn_
Cuts at: 4117 5213 5423 4117
Size: 1096 210 5884
Fragments arranged by size:
5884 1096 210
SbfI CC_TGCA'GG
Cuts at: 4237 4237
Size: 7190
ScaI AGT'ACT
Cuts at: 6707 6707
Size: 7190
SfcI C'TryA_G
Cuts at: 33 235 2008 2727 2866 4233 4918 5911 6102
Size: 202 1773 719 139 1367 685 993 191
Cuts at: 6102 33
Size: 1121
Fragments arranged by size:
1773 1367 1121 993 719 685 202 191
139
SfoI GGC'GCC
Cuts at: 196 4622 4870 196
Size: 4426 248 2516
Fragments arranged by size:
4426 2516 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7190
SmaI CCC'GGG
Cuts at: 1623 2903 4004 4212 1623
Size: 1280 1101 208 4601
Fragments arranged by size:
4601 1280 1101 208
SmlI C'TyrA_G
Cuts at: 985 1487 2131 2400 4345 5260 5752 6014 985
Size: 502 644 269 1945 915 492 262 2161
Fragments arranged by size:
2161 1945 915 644 502 492 269 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7190
SphI G_CATG'C
Cuts at: 4243 4743 4243
Size: 500 6690
StuI AGG'CCT
Cuts at: 1574 4010 1574
Size: 2436 4754
StyI C'CwwG_G
Cuts at: 188 565 682 3094 3692 3859 4070 4324 4678
Size: 377 117 2412 598 167 211 254 354
Cuts at: 4678 188
Size: 2700
Fragments arranged by size:
2700 2412 598 377 354 254 211 167
117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1252 2020 2787 2894 3301 4773 4906 5365 5574
Size: 768 767 107 407 1472 133 459 209
Cuts at: 5574 1252
Size: 2868
Fragments arranged by size:
2868 1472 768 767 459 407 209 133
107
TatI w'GTAC_w
Cuts at: 110 1373 6705 110
Size: 1263 5332 595
Fragments arranged by size:
5332 1263 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 749 781 828 860 3998 4030 6707 6739 749
Size: 32 47 32 3138 32 2677 32 1200
Fragments arranged by size:
3138 2677 1200 47 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3667 4987 3667
Size: 1320 5870
XbaI T'CTAG_A
Cuts at: 4221 4221
Size: 7190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4685 4685
Size: 7190
XmaI C'CCGG_G
Cuts at: 1621 2901 4002 4210 1621
Size: 1280 1101 208 4601
Fragments arranged by size:
4601 1280 1101 208
XmnI GAAnn'nnTTC
Cuts at: 3042 3813 3042
Size: 771 6419
ZraI GAC'GTC
Cuts at: 284 284
Size: 7190
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BfrBI BmgBI BmtI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI