Restriction map for
pF1KA1469
(Circular) (Six-base) MAPSORT of: pF1KA1469.seq Check: 929 from: 1 to: 5060
pF1KA1469 5060 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2098 2098
Size: 5060
Acc65I G'GTAC_C
Cuts at: 2076 2076
Size: 5060
AclI AA'CG_TT
Cuts at: 4900 4900
Size: 5060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 320 2885 3317 4064 4332 286
Size: 34 2565 432 747 268 1014
Fragments arranged by size:
2565 1014 747 432 268 34
AflIII A'CryG_T
Cuts at: 2412 3516 4493 2412
Size: 1104 977 2979
Fragments arranged by size:
2979 1104 977
AgeI A'CCGG_T
Cuts at: 3448 3448
Size: 5060
AlwNI CAG_nnn'CTG
Cuts at: 1840 2365 3932 1840
Size: 525 1567 2968
Fragments arranged by size:
2968 1567 525
ApaLI G'TGCA_C
Cuts at: 3830 3830
Size: 5060
ApoI r'AATT_y
Cuts at: 397 485 810 2064 4413 4669 397
Size: 88 325 1254 2349 256 788
Fragments arranged by size:
2349 1254 788 325 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 5060
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5060
AvaI C'yCGr_G
Cuts at: 2080 2080
Size: 5060
BamHI G'GATC_C
Cuts at: 2085 2085
Size: 5060
BanI G'GyrC_C
Cuts at: 891 2076 2490 2738 2773 891
Size: 1185 414 248 35 3178
Fragments arranged by size:
3178 1185 414 248 35
BanII G_rGCy'C
Cuts at: 1103 2074 3104 1103
Size: 971 1030 3059
Fragments arranged by size:
3059 1030 971
BbeI G_GCGC'C
Cuts at: 2494 2742 2494
Size: 248 4812
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 910 944 910
Size: 34 5026
BciVI GTATCCnnnnn_n'
Cuts at: 1241 2958 3719 5060 1241
Size: 1717 761 1341 1241
Fragments arranged by size:
1717 1341 1241 761
BclI T'GATC_A
Cuts at: 110 2580 110
Size: 2470 2590
BfrBI ATG'CAT
Cuts at: 1541 1541
Size: 5060
BglII A'GATC_T
Cuts at: 2575 4612 2575
Size: 2037 3023
BlpI GC'TnA_GC
Cuts at: 2172 2172
Size: 5060
Bme1580I G_kGCm'C
Cuts at: 896 2685 2778 3834 896
Size: 1789 93 1056 2122
Fragments arranged by size:
2122 1789 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1444 2272 2353 2688 4832 1444
Size: 828 81 335 2144 1672
Fragments arranged by size:
2144 1672 828 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3427 3427
Size: 5060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 1241 1534 144
Size: 1097 293 3670
Fragments arranged by size:
3670 1097 293
Bpu10I CC'TnA_GC
Cuts at: 604 604
Size: 5060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1311 1770 2236 3115 3607 3905 1311
Size: 459 466 879 492 298 2466
Fragments arranged by size:
2466 879 492 466 459 298
BsaI GGTCTCn'nnnn_
Cuts at: 703 4764 703
Size: 4061 999
BsaAI yAC'GTr
Cuts at: 1057 2413 1057
Size: 1356 3704
BsaBI GATnn'nnATC
Cuts at: 6 659 2574 4611 6
Size: 653 1915 2037 455
Fragments arranged by size:
2037 1915 653 455
BsaHI Gr'CG_yC
Cuts at: 2491 2739 3441 4937 2491
Size: 248 702 1496 2614
Fragments arranged by size:
2614 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2249 2770 3448 3722 3869 4280 2249
Size: 521 678 274 147 411 3029
Fragments arranged by size:
3029 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1827 1942 1827
Size: 115 4945
BseYI C'CCAG_C
Cuts at: 325 2485 3243 3820 4316 325
Size: 2160 758 577 496 1069
Fragments arranged by size:
2160 1069 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 1595 809
Size: 786 4274
BsiEI CG_ry'CG
Cuts at: 104 2602 2648 3856 104
Size: 2498 46 1208 1308
Fragments arranged by size:
2498 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2074 2852 3042 3834 2074
Size: 778 190 792 3300
Fragments arranged by size:
3300 792 778 190
BsmI GAATG_Cn'
Cuts at: 553 553
Size: 5060
Bsp1286I G_dGCh'C
Cuts at: 896 1103 2074 2685 2778 2852 3042 3104 3834
Size: 207 971 611 93 74 190 62 730
Cuts at: 3834 896
Size: 2122
Fragments arranged by size:
2122 971 730 611 207 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4280 4280
Size: 5060
BspHI T'CATG_A
Cuts at: 4236 4236
Size: 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2110 2324 2626 3007 2110
Size: 214 302 381 4163
Fragments arranged by size:
4163 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3352 3406 4890 5055 50
Size: 3302 54 1484 165 55
Fragments arranged by size:
3302 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 1914 2972 1730
Size: 184 1058 3818
Fragments arranged by size:
3818 1058 184
BsrFI r'CCGG_y
Cuts at: 3058 3239 3448 3058
Size: 181 209 4670
Fragments arranged by size:
4670 209 181
BsrGI T'GTAC_A
Cuts at: 1625 1973 1625
Size: 348 4712
BssSI C'ACGA_G
Cuts at: 3331 3689 3331
Size: 358 4702
BstAPI GCAn_nnn'nTGC
Cuts at: 2437 2437
Size: 5060
BstBI TT'CG_AA
Cuts at: 1620 3421 1620
Size: 1801 3259
BstEII G'GTnAC_C
Cuts at: 1725 1725
Size: 5060
BstYI r'GATC_y
Cuts at: 1 1146 1456 2085 2552 2575 2910 3156 4168
Size: 1145 310 629 467 23 335 246 1012
Cuts at: 4168 4254 4266 4612 1
Size: 86 12 346 449
Fragments arranged by size:
1145 1012 629 467 449 346 335 310
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 853 968 853
Size: 115 4945
BtgI C'CryG_G
Cuts at: 1550 1550
Size: 5060
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 233 3010 3191 3354 233
Size: 2777 181 163 1939
Fragments arranged by size:
2777 1939 181 163
BtsI GCAGTG_nn'
Cuts at: 1208 1497 2303 1208
Size: 289 806 3965
Fragments arranged by size:
3965 806 289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1077 1112 1228 1263 1077
Size: 35 116 35 4874
Fragments arranged by size:
4874 116 35 35
DraI TTT'AAA
Cuts at: 2059 2059
Size: 5060
DrdI GACnn_nn'nnGTC
Cuts at: 523 795 2766 3624 523
Size: 272 1971 858 1959
Fragments arranged by size:
1971 1959 858 272
EaeI y'GGCC_r
Cuts at: 1249 2645 2819 3210 3237 4582 1249
Size: 1396 174 391 27 1345 1727
Fragments arranged by size:
1727 1396 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2645 2645
Size: 5060
EarI CTCTTCn'nnn_
Cuts at: 539 695 1786 3083 3293 539
Size: 156 1091 1297 210 2306
Fragments arranged by size:
2306 1297 1091 210 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3268 3578 3724 4870 3268
Size: 310 146 1146 3458
Fragments arranged by size:
3458 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2072 2072
Size: 5060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 286 320 1241 1534 2885 3317 4064 4332
Size: 142 34 921 293 1351 432 747 268
Cuts at: 4332 144
Size: 872
Fragments arranged by size:
1351 921 872 747 432 293 268 142
34
EcoNI CCTnn'n_nnAGG
Cuts at: 1238 1238
Size: 5060
EcoO109I rG'GnC_Cy
Cuts at: 2199 2199
Size: 5060
EcoRI G'AATT_C
Cuts at: 810 2064 810
Size: 1254 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 5060
FspI TGC'GCA
Cuts at: 2841 2841
Size: 5060
HaeII r_GCGC'y
Cuts at: 2494 2742 3764 4743 2494
Size: 248 1022 979 2811
Fragments arranged by size:
2811 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 505 537 2902 2932 2934 2964 505
Size: 32 2365 30 2 30 2601
Fragments arranged by size:
2601 2365 32 30 30 2
HincII GTy'rAC
Cuts at: 1089 2099 1089
Size: 1010 4050
HindIII A'AGCT_T
Cuts at: 859 859
Size: 5060
KasI G'GCGC_C
Cuts at: 2490 2738 2490
Size: 248 4812
KpnI G_GTAC'C
Cuts at: 2080 2080
Size: 5060
MfeI C'AATT_G
Cuts at: 1472 1472
Size: 5060
MluI A'CGCG_T
Cuts at: 4493 4493
Size: 5060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 289 600 3731 3915 289
Size: 311 3131 184 1434
Fragments arranged by size:
3131 1434 311 184
MscI TGG'CCA
Cuts at: 1251 2821 1251
Size: 1570 3490
MslI CAynn'nnrTG
Cuts at: 207 512 1769 3049 3176 207
Size: 305 1257 1280 127 2091
Fragments arranged by size:
2091 1280 1257 305 127
MspA1I CmG'CkG
Cuts at: 1424 2171 2485 2845 3858 4103 1424
Size: 747 314 360 1013 245 2381
Fragments arranged by size:
2381 1013 747 360 314 245
NaeI GCC'GGC
Cuts at: 3241 3241
Size: 5060
NarI GG'CG_CC
Cuts at: 2491 2739 2491
Size: 248 4812
NcoI C'CATG_G
Cuts at: 1550 1550
Size: 5060
NgoMIV G'CCGG_C
Cuts at: 3239 3239
Size: 5060
NsiI A_TGCA'T
Cuts at: 1543 1543
Size: 5060
NspI r_CATG'y
Cuts at: 2113 2613 3520 2113
Size: 500 907 3653
Fragments arranged by size:
3653 907 500
PasI CC'CwG_GG
Cuts at: 617 617
Size: 5060
PciI A'CATG_T
Cuts at: 3516 3516
Size: 5060
PfoI T'CCnGG_A
Cuts at: 1865 1865
Size: 5060
PmeI GTTT'AAAC
Cuts at: 2059 2059
Size: 5060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 4199
Size: 32 5028
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1529 1561 1529
Size: 32 5028
PstI C_TGCA'G
Cuts at: 534 2107 2792 534
Size: 1573 685 2802
Fragments arranged by size:
2802 1573 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5060
PvuII CAG'CTG
Cuts at: 1424 2485 2845 1424
Size: 1061 360 3639
Fragments arranged by size:
3639 1061 360
RsrII CG'GwC_CG
Cuts at: 3255 3255
Size: 5060
SacI G_AGCT'C
Cuts at: 2074 2074
Size: 5060
SalI G'TCGA_C
Cuts at: 2097 2097
Size: 5060
SapI GCTCTTCn'nnn_
Cuts at: 1786 3083 3293 1786
Size: 1297 210 3553
Fragments arranged by size:
3553 1297 210
SbfI CC_TGCA'GG
Cuts at: 2107 2107
Size: 5060
ScaI AGT'ACT
Cuts at: 1498 4577 1498
Size: 3079 1981
SfcI C'TryA_G
Cuts at: 33 530 635 2013 2103 2788 3781 3972 33
Size: 497 105 1378 90 685 993 191 1121
Fragments arranged by size:
1378 1121 993 685 497 191 105 90
SfoI GGC'GCC
Cuts at: 2492 2740 2492
Size: 248 4812
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5060
SmaI CCC'GGG
Cuts at: 2082 2082
Size: 5060
SmlI C'TyrA_G
Cuts at: 1326 1785 2215 3130 3622 3884 1326
Size: 459 430 915 492 262 2502
Fragments arranged by size:
2502 915 492 459 430 262
SpeI A'CTAG_T
Cuts at: 67 703 67
Size: 636 4424
SphI G_CATG'C
Cuts at: 2113 2613 2113
Size: 500 4560
StyI C'CwwG_G
Cuts at: 1239 1550 2194 2548 1239
Size: 311 644 354 3751
Fragments arranged by size:
3751 644 354 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2643 2776 3235 3444 2643
Size: 133 459 209 4259
Fragments arranged by size:
4259 459 209 133
TatI w'GTAC_w
Cuts at: 1496 1625 1973 4575 1496
Size: 129 348 2602 1981
Fragments arranged by size:
2602 1981 348 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1215 1247 1305 1337 1935 1967 4577 4609 1215
Size: 32 58 32 598 32 2610 32 1666
Fragments arranged by size:
2610 1666 598 58 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2857 2857
Size: 5060
XbaI T'CTAG_A
Cuts at: 723 2091 723
Size: 1368 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1689 2555 1689
Size: 866 4194
XmaI C'CCGG_G
Cuts at: 2080 2080
Size: 5060
XmnI GAAnn'nnTTC
Cuts at: 1124 1124
Size: 5060
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BssHII BstXI BstZ17I ClaI
DraIII EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI PacI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI