Restriction map for
pF1KA1472
(Circular) (Six-base) MAPSORT of: pF1KA1472.seq Check: 8780 from: 1 to: 5102
pF1KA1472 5102 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 601 2140 601
Size: 1539 3563
Acc65I G'GTAC_C
Cuts at: 2118 2118
Size: 5102
AclI AA'CG_TT
Cuts at: 4942 4942
Size: 5102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1210 2927 3359 3712 4374 982
Size: 228 1717 432 353 662 1710
Fragments arranged by size:
1717 1710 662 432 353 228
AflII C'TTAA_G
Cuts at: 1012 1012
Size: 5102
AflIII A'CryG_T
Cuts at: 857 2454 4254 4535 857
Size: 1597 1800 281 1424
Fragments arranged by size:
1800 1597 1424 281
AhdI GACnn_n'nnGTC
Cuts at: 720 720
Size: 5102
AlwNI CAG_nnn'CTG
Cuts at: 241 329 394 1465 2042 2407 3845 241
Size: 88 65 1071 577 365 1438 1498
Fragments arranged by size:
1498 1438 1071 577 365 88 65
ApaI G_GGCC'C
Cuts at: 116 116
Size: 5102
ApaLI G'TGCA_C
Cuts at: 3940 3940
Size: 5102
ApoI r'AATT_y
Cuts at: 179 2106 4455 4711 179
Size: 1927 2349 256 570
Fragments arranged by size:
2349 1927 570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5102
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
AvaI C'yCGr_G
Cuts at: 558 1653 2122 558
Size: 1095 469 3538
Fragments arranged by size:
3538 1095 469
BamHI G'GATC_C
Cuts at: 2007 2127 2007
Size: 120 4982
BanI G'GyrC_C
Cuts at: 1584 2118 2532 2780 2815 1584
Size: 534 414 248 35 3871
Fragments arranged by size:
3871 534 414 248 35
BanII G_rGCy'C
Cuts at: 116 751 1613 2116 3146 116
Size: 635 862 503 1030 2072
Fragments arranged by size:
2072 1030 862 635 503
BbeI G_GCGC'C
Cuts at: 2536 2784 2536
Size: 248 4854
BbsI GAAGACnn'nnnn_
Cuts at: 385 614 385
Size: 229 4873
BciVI GTATCCnnnnn_n'
Cuts at: 3000 4056 5102 3000
Size: 1056 1046 3000
Fragments arranged by size:
3000 1056 1046
BclI T'GATC_A
Cuts at: 2622 2622
Size: 5102
BglI GCCn_nnn'nGGC
Cuts at: 410 1081 410
Size: 671 4431
BglII A'GATC_T
Cuts at: 2617 4654 2617
Size: 2037 3065
BlpI GC'TnA_GC
Cuts at: 300 745 2214 300
Size: 445 1469 3188
Fragments arranged by size:
3188 1469 445
Bme1580I G_kGCm'C
Cuts at: 116 1589 2727 2820 3944 116
Size: 1473 1138 93 1124 1274
Fragments arranged by size:
1473 1274 1138 1124 93
BmgBI CAC'GTC
Cuts at: 1581 1581
Size: 5102
BmrI ACTGGGnnnn_n'
Cuts at: 761 1071 2314 2395 2730 4874 761
Size: 310 1243 81 335 2144 989
Fragments arranged by size:
2144 1243 989 335 310 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1731 3469 1731
Size: 1738 3364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 1495 1531 1621 1935 1234
Size: 261 36 90 314 4401
Fragments arranged by size:
4401 314 261 90 36
Bpu10I CC'TnA_GC
Cuts at: 2035 2035
Size: 5102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2278 3157 3871 4169 2278
Size: 879 714 298 3211
Fragments arranged by size:
3211 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1774 4806 1774
Size: 3032 2070
BsaAI yAC'GTr
Cuts at: 2455 2455
Size: 5102
BsaBI GATnn'nnATC
Cuts at: 6 2616 4653 6
Size: 2610 2037 455
Fragments arranged by size:
2610 2037 455
BsaHI Gr'CG_yC
Cuts at: 1663 1898 2533 2781 3483 4979 1663
Size: 235 635 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 635 248 235
BsaWI w'CCGG_w
Cuts at: 2291 2812 3490 3901 4048 4322 2291
Size: 521 678 411 147 274 3071
Fragments arranged by size:
3071 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 790 820 1000 1030 790
Size: 30 180 30 4862
Fragments arranged by size:
4862 180 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 546 1066 1069 1555 546
Size: 520 3 486 4093
Fragments arranged by size:
4093 520 486 3
BseYI C'CCAG_C
Cuts at: 234 283 1019 1596 2527 3285 3950 4358 234
Size: 49 736 577 931 758 665 408 978
Fragments arranged by size:
978 931 758 736 665 577 408 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 753 166
Size: 587 4515
BsiEI CG_ry'CG
Cuts at: 104 2644 2690 3920 104
Size: 2540 46 1230 1286
Fragments arranged by size:
2540 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 140 1613 2116 2894 3084 3944 140
Size: 1473 503 778 190 860 1298
Fragments arranged by size:
1473 1298 860 778 503 190
BsmI GAATG_Cn'
Cuts at: 482 1169 1485 1981 2061 482
Size: 687 316 496 80 3523
Fragments arranged by size:
3523 687 496 316 80
Bsp1286I G_dGCh'C
Cuts at: 116 140 751 1589 1613 2116 2727 2820 2894
Size: 24 611 838 24 503 611 93 74
Cuts at: 2894 3084 3146 3944 116
Size: 190 62 798 1274
Fragments arranged by size:
1274 838 798 611 611 503 190 93
74 62 24 24
BspHI T'CATG_A
Cuts at: 153 836 1826 3534 153
Size: 683 990 1708 1721
Fragments arranged by size:
1721 1708 990 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1353 2152 2366 2668 3049 1353
Size: 799 214 302 381 3406
Fragments arranged by size:
3406 799 381 302 214
BsrBI CCG'CTC
Cuts at: 50 583 3394 3448 4932 5097 50
Size: 533 2811 54 1484 165 55
Fragments arranged by size:
2811 1484 533 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3014 3014
Size: 5102
BsrFI r'CCGG_y
Cuts at: 3100 3281 3100
Size: 181 4921
BssSI C'ACGA_G
Cuts at: 3373 4081 3373
Size: 708 4394
BstAPI GCAn_nnn'nTGC
Cuts at: 144 2479 144
Size: 2335 2767
BstBI TT'CG_AA
Cuts at: 3463 3463
Size: 5102
BstEII G'GTnAC_C
Cuts at: 1468 1468
Size: 5102
BstXI CCAn_nnnn'nTGG
Cuts at: 1530 1530
Size: 5102
BstYI r'GATC_y
Cuts at: 1 1935 2007 2127 2594 2617 2952 3198 3504
Size: 1934 72 120 467 23 335 246 306
Cuts at: 3504 3516 3602 4654 1
Size: 12 86 1052 449
Fragments arranged by size:
1934 1052 467 449 335 306 246 120
86 72 23 12
Bsu36I CC'TnA_GG
Cuts at: 587 587
Size: 5102
BtgI C'CryG_G
Cuts at: 107 703 1775 1958 1967 107
Size: 596 1072 183 9 3242
Fragments arranged by size:
3242 1072 596 183 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3052 3233 3396 3052
Size: 181 163 4758
Fragments arranged by size:
4758 181 163
BtsI GCAGTG_nn'
Cuts at: 916 1554 2345 916
Size: 638 791 3673
Fragments arranged by size:
3673 791 638
DraI TTT'AAA
Cuts at: 2101 2101
Size: 5102
DraIII CAC_nnn'GTG
Cuts at: 144 228 1249 144
Size: 84 1021 3997
Fragments arranged by size:
3997 1021 84
DrdI GACnn_nn'nnGTC
Cuts at: 2808 4152 2808
Size: 1344 3758
EaeI y'GGCC_r
Cuts at: 2687 2861 3252 3279 4624 2687
Size: 174 391 27 1345 3165
Fragments arranged by size:
3165 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2687 2687
Size: 5102
EarI CTCTTCn'nnn_
Cuts at: 257 1297 1352 1853 3125 3335 257
Size: 1040 55 501 1272 210 2024
Fragments arranged by size:
2024 1272 1040 501 210 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 669 2059 3310 4052 4198 4912 669
Size: 1390 1251 742 146 714 859
Fragments arranged by size:
1390 1251 859 742 714 146
EcoICRI GAG'CTC
Cuts at: 1611 2114 1611
Size: 503 4599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1210 1234 1495 1531 1621 1935 2927 3359
Size: 228 24 261 36 90 314 992 432
Cuts at: 3359 3712 4374 982
Size: 353 662 1710
Fragments arranged by size:
1710 992 662 432 353 314 261 228
90 36 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2034 2034
Size: 5102
EcoO109I rG'GnC_Cy
Cuts at: 112 113 643 714 1641 2027 2241 112
Size: 1 530 71 927 386 214 2973
Fragments arranged by size:
2973 927 530 386 214 71 1
EcoRI G'AATT_C
Cuts at: 179 2106 179
Size: 1927 3175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 5102
FspI TGC'GCA
Cuts at: 2883 2883
Size: 5102
HaeII r_GCGC'y
Cuts at: 300 978 2536 2784 4014 4785 300
Size: 678 1558 248 1230 771 617
Fragments arranged by size:
1558 1230 771 678 617 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 706 738 1000 1032 1333 1365 2944
Size: 32 136 32 262 32 301 32 1579
Cuts at: 2944 2974 2976 3006 538
Size: 30 2 30 2634
Fragments arranged by size:
2634 1579 301 262 136 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 602 2141 602
Size: 1539 3563
KasI G'GCGC_C
Cuts at: 2532 2780 2532
Size: 248 4854
KpnI G_GTAC'C
Cuts at: 2122 2122
Size: 5102
MluI A'CGCG_T
Cuts at: 4535 4535
Size: 5102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1847 3861 4045 1847
Size: 2014 184 2904
Fragments arranged by size:
2904 2014 184
MscI TGG'CCA
Cuts at: 2863 2863
Size: 5102
MslI CAynn'nnrTG
Cuts at: 1237 1528 3091 3218 1237
Size: 291 1563 127 3121
Fragments arranged by size:
3121 1563 291 127
MspA1I CmG'CkG
Cuts at: 705 744 1023 2213 2527 2887 3671 3916 705
Size: 39 279 1190 314 360 784 245 1891
Fragments arranged by size:
1891 1190 784 360 314 279 245 39
NaeI GCC'GGC
Cuts at: 3283 3283
Size: 5102
NarI GG'CG_CC
Cuts at: 2533 2781 2533
Size: 248 4854
NcoI C'CATG_G
Cuts at: 107 107
Size: 5102
NdeI CA'TA_TG
Cuts at: 206 596 206
Size: 390 4712
NgoMIV G'CCGG_C
Cuts at: 3281 3281
Size: 5102
NspI r_CATG'y
Cuts at: 861 1035 2155 2655 4258 861
Size: 174 1120 500 1603 1705
Fragments arranged by size:
1705 1603 1120 500 174
PasI CC'CwG_GG
Cuts at: 1495 1495
Size: 5102
PciI A'CATG_T
Cuts at: 857 4254 857
Size: 3397 1705
PfoI T'CCnGG_A
Cuts at: 1508 1637 1508
Size: 129 4973
PmeI GTTT'AAAC
Cuts at: 2101 2101
Size: 5102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 5070
PpuMI rG'GwC_Cy
Cuts at: 643 714 1641 643
Size: 71 927 4104
Fragments arranged by size:
4104 927 71
PshAI GACnn'nnGTC
Cuts at: 1646 1646
Size: 5102
PspOMI G'GGCC_C
Cuts at: 112 112
Size: 5102
PspXI vC'TCGA_Gb
Cuts at: 558 1653 558
Size: 1095 4007
PstI C_TGCA'G
Cuts at: 923 1631 2149 2834 923
Size: 708 518 685 3191
Fragments arranged by size:
3191 708 685 518
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5102
PvuII CAG'CTG
Cuts at: 744 1023 2527 2887 744
Size: 279 1504 360 2959
Fragments arranged by size:
2959 1504 360 279
RsrII CG'GwC_CG
Cuts at: 3297 3297
Size: 5102
SacI G_AGCT'C
Cuts at: 1613 2116 1613
Size: 503 4599
SacII CC_GC'GG
Cuts at: 706 706
Size: 5102
SalI G'TCGA_C
Cuts at: 600 2139 600
Size: 1539 3563
SapI GCTCTTCn'nnn_
Cuts at: 1297 1352 3125 3335 1297
Size: 55 1773 210 3064
Fragments arranged by size:
3064 1773 210 55
SbfI CC_TGCA'GG
Cuts at: 2149 2149
Size: 5102
ScaI AGT'ACT
Cuts at: 1912 1989 4619 1912
Size: 77 2630 2395
Fragments arranged by size:
2630 2395 77
SexAI A'CCwGG_T
Cuts at: 531 531
Size: 5102
SfcI C'TryA_G
Cuts at: 33 825 919 1590 1627 2145 2830 3798 3989
Size: 792 94 671 37 518 685 968 191
Cuts at: 3989 33
Size: 1146
Fragments arranged by size:
1146 968 792 685 671 518 191 94
37
SfoI GGC'GCC
Cuts at: 2534 2782 2534
Size: 248 4854
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
SmaI CCC'GGG
Cuts at: 2124 2124
Size: 5102
SmlI C'TyrA_G
Cuts at: 558 1012 1653 2257 3172 3886 4148 558
Size: 454 641 604 915 714 262 1512
Fragments arranged by size:
1512 915 714 641 604 454 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5102
SphI G_CATG'C
Cuts at: 1035 2155 2655 1035
Size: 1120 500 3482
Fragments arranged by size:
3482 1120 500
SspI AAT'ATT
Cuts at: 1180 1180
Size: 5102
StuI AGG'CCT
Cuts at: 521 521
Size: 5102
StyI C'CwwG_G
Cuts at: 107 2236 2590 107
Size: 2129 354 2619
Fragments arranged by size:
2619 2129 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1591 2685 2818 3277 3486 1591
Size: 1094 133 459 209 3207
Fragments arranged by size:
3207 1094 459 209 133
TatI w'GTAC_w
Cuts at: 1910 1987 4617 1910
Size: 77 2630 2395
Fragments arranged by size:
2630 2395 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 214 246 333 365 1255 1287 4619 4651 214
Size: 32 87 32 890 32 3332 32 665
Fragments arranged by size:
3332 890 665 87 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2899 2899
Size: 5102
XbaI T'CTAG_A
Cuts at: 2133 2133
Size: 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2597 2597
Size: 5102
XhoI C'TCGA_G
Cuts at: 558 1653 558
Size: 1095 4007
XmaI C'CCGG_G
Cuts at: 2122 2122
Size: 5102
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmBI
BspEI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PflMI PmlI PsiI PsrI SanDI SfiI SgrAI
SnaBI SrfI SwaI XmnI ZraI