FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KA1475, 973 aa 1>>>pF1KA1475 973 - 973 aa - 973 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 5.4551+/-0.000356; mu= 21.5715+/- 0.022 mean_var=88.0182+/-17.660, 0's: 0 Z-trim(114.8): 25 B-trim: 57 in 1/52 Lambda= 0.136706 statistics sampled from 24883 (24908) to 24883 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.67), E-opt: 0.2 (0.292), width: 16 Scan time: 11.650 The best scores are: opt bits E(85289) NP_065908 (OMIM: 608551) vacuolar protein sorting- ( 973) 6555 1303.6 0 XP_011520145 (OMIM: 608551) PREDICTED: vacuolar pr ( 901) 6052 1204.4 0 XP_011520146 (OMIM: 608551) PREDICTED: vacuolar pr ( 871) 5890 1172.4 0 XP_016877936 (OMIM: 608551) PREDICTED: vacuolar pr ( 605) 4082 815.7 0 XP_016867477 (OMIM: 605485) PREDICTED: vacuolar pr ( 469) 174 44.9 0.0012 NP_542198 (OMIM: 605485) vacuolar protein sorting- ( 829) 174 45.1 0.0019 NP_055211 (OMIM: 605485) vacuolar protein sorting- ( 854) 174 45.1 0.0019 >>NP_065908 (OMIM: 608551) vacuolar protein sorting-asso (973 aa) initn: 6555 init1: 6555 opt: 6555 Z-score: 6983.5 bits: 1303.6 E(85289): 0 Smith-Waterman score: 6555; 100.0% identity (100.0% similar) in 973 aa overlap (1-973:1-973) 10 20 30 40 50 60 pF1KA1 MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSL 10 20 30 40 50 60 70 80 90 100 110 120 pF1KA1 VVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 VVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTE 70 80 90 100 110 120 130 140 150 160 170 180 pF1KA1 VLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 VLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGG 130 140 150 160 170 180 190 200 210 220 230 240 pF1KA1 LFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 LFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAE 190 200 210 220 230 240 250 260 270 280 290 300 pF1KA1 AQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 AQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCG 250 260 270 280 290 300 310 320 330 340 350 360 pF1KA1 RPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 RPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFL 310 320 330 340 350 360 370 380 390 400 410 420 pF1KA1 EKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 EKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPD 370 380 390 400 410 420 430 440 450 460 470 480 pF1KA1 CLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 CLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASL 430 440 450 460 470 480 490 500 510 520 530 540 pF1KA1 KPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 KPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASR 490 500 510 520 530 540 550 560 570 580 590 600 pF1KA1 ASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 ASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSP 550 560 570 580 590 600 610 620 630 640 650 660 pF1KA1 ILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 ILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETE 610 620 630 640 650 660 670 680 690 700 710 720 pF1KA1 QAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 QAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVL 670 680 690 700 710 720 730 740 750 760 770 780 pF1KA1 ELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 ELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASC 730 740 750 760 770 780 790 800 810 820 830 840 pF1KA1 PLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQEL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 PLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQEL 790 800 810 820 830 840 850 860 870 880 890 900 pF1KA1 RGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 RGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQR 850 860 870 880 890 900 910 920 930 940 950 960 pF1KA1 KLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_065 KLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFI 910 920 930 940 950 960 970 pF1KA1 DPQRYEEEQLSWL ::::::::::::: NP_065 DPQRYEEEQLSWL 970 >>XP_011520145 (OMIM: 608551) PREDICTED: vacuolar protei (901 aa) initn: 6052 init1: 6052 opt: 6052 Z-score: 6447.8 bits: 1204.4 E(85289): 0 Smith-Waterman score: 6052; 100.0% identity (100.0% similar) in 895 aa overlap (79-973:7-901) 50 60 70 80 90 100 pF1KA1 IDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHT :::::::::::::::::::::::::::::: XP_011 MYISGNIDLGKANEPNHVELGRKDDAKVHKMFLDHT 10 20 30 110 120 130 140 150 160 pF1KA1 GSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 GSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGH 40 50 60 70 80 90 170 180 190 200 210 220 pF1KA1 IFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 IFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRL 100 110 120 130 140 150 230 240 250 260 270 280 pF1KA1 FQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 FQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMM 160 170 180 190 200 210 290 300 310 320 330 340 pF1KA1 GDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 GDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCT 220 230 240 250 260 270 350 360 370 380 390 400 pF1KA1 LTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 LTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRF 280 290 300 310 320 330 410 420 430 440 450 460 pF1KA1 DLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 DLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEA 340 350 360 370 380 390 470 480 490 500 510 520 pF1KA1 LAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 LAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLS 400 410 420 430 440 450 530 540 550 560 570 580 pF1KA1 SPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLAR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 SPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLAR 460 470 480 490 500 510 590 600 610 620 630 640 pF1KA1 HRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRY :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 HRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRY 520 530 540 550 560 570 650 660 670 680 690 700 pF1KA1 MEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 MEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHG 580 590 600 610 620 630 710 720 730 740 750 760 pF1KA1 HHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 HHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEE 640 650 660 670 680 690 770 780 790 800 810 820 pF1KA1 DVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 DVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATA 700 710 720 730 740 750 830 840 850 860 870 880 pF1KA1 SAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 SAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLP 760 770 780 790 800 810 890 900 910 920 930 940 pF1KA1 AYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 AYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCG 820 830 840 850 860 870 950 960 970 pF1KA1 ELMIRSIDRPFIDPQRYEEEQLSWL ::::::::::::::::::::::::: XP_011 ELMIRSIDRPFIDPQRYEEEQLSWL 880 890 900 >>XP_011520146 (OMIM: 608551) PREDICTED: vacuolar protei (871 aa) initn: 5890 init1: 5890 opt: 5890 Z-score: 6275.4 bits: 1172.4 E(85289): 0 Smith-Waterman score: 5890; 100.0% identity (100.0% similar) in 871 aa overlap (103-973:1-871) 80 90 100 110 120 130 pF1KA1 KDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVR :::::::::::::::::::::::::::::: XP_011 MFLDHTGSHLLIALSSTEVLYVNRNGQKVR 10 20 30 140 150 160 170 180 190 pF1KA1 PLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 PLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRP 40 50 60 70 80 90 200 210 220 230 240 250 pF1KA1 LYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 LYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYT 100 110 120 130 140 150 260 270 280 290 300 310 pF1KA1 DHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVW :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 DHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVW 160 170 180 190 200 210 320 330 340 350 360 370 pF1KA1 EYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 EYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKD 220 230 240 250 260 270 380 390 400 410 420 430 pF1KA1 SSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 SSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADF 280 290 300 310 320 330 440 450 460 470 480 490 pF1KA1 CFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 CFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLT 340 350 360 370 380 390 500 510 520 530 540 550 pF1KA1 TWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 TWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGD 400 410 420 430 440 450 560 570 580 590 600 610 pF1KA1 TEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 TEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVD 460 470 480 490 500 510 620 630 640 650 660 670 pF1KA1 AWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 AWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYA 520 530 540 550 560 570 680 690 700 710 720 730 pF1KA1 RGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 RGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQ 580 590 600 610 620 630 740 750 760 770 780 790 pF1KA1 VDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 VDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFF 640 650 660 670 680 690 800 810 820 830 840 850 pF1KA1 PDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 PDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDK 700 710 720 730 740 750 860 870 880 890 900 910 pF1KA1 CATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 CATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGS 760 770 780 790 800 810 920 930 940 950 960 970 pF1KA1 ARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSW :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_011 ARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSW 820 830 840 850 860 870 pF1KA1 L : XP_011 L >>XP_016877936 (OMIM: 608551) PREDICTED: vacuolar protei (605 aa) initn: 4082 init1: 4082 opt: 4082 Z-score: 4350.3 bits: 815.7 E(85289): 0 Smith-Waterman score: 4082; 100.0% identity (100.0% similar) in 605 aa overlap (369-973:1-605) 340 350 360 370 380 390 pF1KA1 LADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDV :::::::::::::::::::::::::::::: XP_016 MVKDSSTGQLWAYTERAVFRYHVQREARDV 10 20 30 400 410 420 430 440 450 pF1KA1 WRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIAL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 WRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIAL 40 50 60 70 80 90 460 470 480 490 500 510 pF1KA1 KFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 KFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRE 100 110 120 130 140 150 520 530 540 550 560 570 pF1KA1 TKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 TKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEA 160 170 180 190 200 210 580 590 600 610 620 630 pF1KA1 YEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 YEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGE 220 230 240 250 260 270 640 650 660 670 680 690 pF1KA1 VQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 VQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLK 280 290 300 310 320 330 700 710 720 730 740 750 pF1KA1 YALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 YALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLK 340 350 360 370 380 390 760 770 780 790 800 810 pF1KA1 IARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 IARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQE 400 410 420 430 440 450 820 830 840 850 860 870 pF1KA1 LQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 LQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADC 460 470 480 490 500 510 880 890 900 910 920 930 pF1KA1 LLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: XP_016 LLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDE 520 530 540 550 560 570 940 950 960 970 pF1KA1 LVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL ::::::::::::::::::::::::::::::::::: XP_016 LVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL 580 590 600 >>XP_016867477 (OMIM: 605485) PREDICTED: vacuolar protei (469 aa) initn: 70 init1: 70 opt: 174 Z-score: 186.3 bits: 44.9 E(85289): 0.0012 Smith-Waterman score: 189; 19.5% identity (49.0% similar) in 473 aa overlap (423-879:11-438) 400 410 420 430 440 pF1KA1 REARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYAL---T : :: . : . . .: . . XP_016 MGAERGENSRDIGLAYINHLVERGDYDIAARKCQKILGKN 10 20 30 40 450 460 470 480 490 500 pF1KA1 QSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGD . .: . :: : : .:.. .: : ::: .: .: : . .. XP_016 AALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPL--IYEMILHEFLESDYEGFATLIREW 50 60 70 80 90 510 520 530 540 550 560 pF1KA1 PEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERV : : . : :.. ... :. . : .. ..:.. . .:... .: .. XP_016 PGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALE-IYLTLRHKDVFQL 100 110 120 130 140 150 570 580 590 600 610 620 pF1KA1 VAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPA . : . .. .. :.. :. .. :: .. .... .... XP_016 IHKHNLFSSIKDKIV---------LLMDFDS-------EKAVDMLLDNEDKISIKKVVEE 160 170 180 190 200 630 640 650 660 670 680 pF1KA1 LVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYAR-GRPDSLLAYLEQAGA : . :.:.: :.. . . : :. .::::. ::. :: .:... XP_016 LEDRP---ELQHV-----YLHKLFKRDHHKGQRYHEKQISLYAEYDRPN-LLPFLRDS-- 210 220 230 240 250 690 700 710 720 730 740 pF1KA1 SPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQV--DVDLAKQCADLPE .: :. ::..: ... . :.. . . . :. . .. ::: : . : : XP_016 ----THCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAK--E 260 270 280 290 300 750 760 770 780 790 800 pF1KA1 EDEELRKKLWLKIARHVVQEEEDVQTAMACLASC--PLLKIEDVLPFFPDFVTIDHFKEA .:. .:: . . ... . . ... :.: :. . . . : ..... XP_016 QDD---GELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRI----KEGMEIPNLRDS 310 320 330 340 350 810 820 830 840 850 860 pF1KA1 ICSSLKAYNHHIQELQREMEEA--TASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLL- . . :. :: .: : :: . .:.. . . ... . . :. .. : .: :.: XP_016 LVKILQDYN--LQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESCLSPILP 360 370 380 390 400 410 870 880 890 900 910 pF1KA1 ---NRPF--YLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAK .:: .: : :::: .:: XP_016 SDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK 420 430 440 450 460 >>NP_542198 (OMIM: 605485) vacuolar protein sorting-asso (829 aa) initn: 99 init1: 70 opt: 174 Z-score: 183.0 bits: 45.1 E(85289): 0.0019 Smith-Waterman score: 197; 19.6% identity (49.1% similar) in 479 aa overlap (417-879:365-798) 390 400 410 420 430 440 pF1KA1 FRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCY .: :: :: . : . . .: NP_542 RDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILDIGLAYINHLVERGDYDIAARKCQ 340 350 360 370 380 390 450 460 470 480 490 500 pF1KA1 AL---TQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRL . . . .: . :: : : .:.. .: : ::: .: .: : . NP_542 KILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPL--IYEMILHEFLESDYEGFA 400 410 420 430 440 450 510 520 530 540 550 560 pF1KA1 GALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIM .. : : . : :.. ... :. . : .. ..:.. . .:... NP_542 TLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALE-IYLTLRH 460 470 480 490 500 510 570 580 590 600 610 620 pF1KA1 QDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDA .: ... : . .. ...: . :. .. :: .. .... NP_542 KDVFQLIHKHNLFSSIKDKIVLL---------MDFDS-------EKAVDMLLDNEDKISI 520 530 540 550 630 640 650 660 670 680 pF1KA1 RQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYAR-GRPDSLLAY .... : . :.:.: :.. . . : :. .::::. ::. :: . NP_542 KKVVEELEDRP---ELQHV-----YLHKLFKRDHHKGQRYHEKQISLYAEYDRPN-LLPF 560 570 580 590 600 690 700 710 720 730 740 pF1KA1 LEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQV--DVDLAKQ :... .: :. ::..: ... . :.. . . . :. . .. ::: : . NP_542 LRDS------THCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIE 610 620 630 640 650 660 750 760 770 780 790 pF1KA1 CADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASC--PLLKIEDVLPFFPDFVTI : :.:. .:: . . ... . . ... :.: :. . . . : NP_542 FA--KEQDD---GELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRI----KEGMEI 670 680 690 700 710 800 810 820 830 840 850 pF1KA1 DHFKEAICSSLKAYNHHIQELQREMEEA--TASAQRIRRDLQELRGRYGTVEPQDKCATC ...... . :. :: .: : :: . .:.. . . ... . . :. .. : .: NP_542 PNLRDSLVKILQDYN--LQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESC 720 730 740 750 760 860 870 880 890 900 910 pF1KA1 DFPLL----NRPF--YLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAK :.: .:: .: : :::: .:: NP_542 LSPILPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK 770 780 790 800 810 820 >>NP_055211 (OMIM: 605485) vacuolar protein sorting-asso (854 aa) initn: 99 init1: 70 opt: 174 Z-score: 182.8 bits: 45.1 E(85289): 0.0019 Smith-Waterman score: 197; 19.6% identity (49.1% similar) in 479 aa overlap (417-879:390-823) 390 400 410 420 430 440 pF1KA1 FRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCY .: :: :: . : . . .: NP_055 RDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILDIGLAYINHLVERGDYDIAARKCQ 360 370 380 390 400 410 450 460 470 480 490 500 pF1KA1 AL---TQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRL . . . .: . :: : : .:.. .: : ::: .: .: : . NP_055 KILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPL--IYEMILHEFLESDYEGFA 420 430 440 450 460 470 510 520 530 540 550 560 pF1KA1 GALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIM .. : : . : :.. ... :. . : .. ..:.. . .:... NP_055 TLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALE-IYLTLRH 480 490 500 510 520 530 570 580 590 600 610 620 pF1KA1 QDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDA .: ... : . .. ...: . :. .. :: .. .... NP_055 KDVFQLIHKHNLFSSIKDKIVLL---------MDFDS-------EKAVDMLLDNEDKISI 540 550 560 570 580 630 640 650 660 670 680 pF1KA1 RQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYAR-GRPDSLLAY .... : . :.:.: :.. . . : :. .::::. ::. :: . NP_055 KKVVEELEDRP---ELQHV-----YLHKLFKRDHHKGQRYHEKQISLYAEYDRPN-LLPF 590 600 610 620 630 690 700 710 720 730 740 pF1KA1 LEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQV--DVDLAKQ :... .: :. ::..: ... . :.. . . . :. . .. ::: : . NP_055 LRDS------THCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIE 640 650 660 670 680 750 760 770 780 790 pF1KA1 CADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASC--PLLKIEDVLPFFPDFVTI : :.:. .:: . . ... . . ... :.: :. . . . : NP_055 FA--KEQDD---GELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRI----KEGMEI 690 700 710 720 730 800 810 820 830 840 850 pF1KA1 DHFKEAICSSLKAYNHHIQELQREMEEA--TASAQRIRRDLQELRGRYGTVEPQDKCATC ...... . :. :: .: : :: . .:.. . . ... . . :. .. : .: NP_055 PNLRDSLVKILQDYN--LQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESC 740 750 760 770 780 790 860 870 880 890 900 910 pF1KA1 DFPLL----NRPF--YLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAK :.: .:: .: : :::: .:: NP_055 LSPILPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK 800 810 820 830 840 850 973 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Fri Nov 4 01:34:19 2016 done: Fri Nov 4 01:34:20 2016 Total Scan time: 11.650 Total Display time: 0.200 Function used was FASTA [36.3.4 Apr, 2011]