Restriction map for
pF1KA1478
(Circular) (Six-base) MAPSORT of: pF1KA1478.seq Check: 7502 from: 1 to: 5009
pF1KA1478 5009 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 731 2047 731
Size: 1316 3693
Acc65I G'GTAC_C
Cuts at: 2025 2025
Size: 5009
AclI AA'CG_TT
Cuts at: 4849 4849
Size: 5009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 658 1069 2834 3266 3619 4281 343
Size: 315 411 1765 432 353 662 1071
Fragments arranged by size:
1765 1071 662 432 411 353 315
AflIII A'CryG_T
Cuts at: 1767 2361 4161 4442 1767
Size: 594 1800 281 2334
Fragments arranged by size:
2334 1800 594 281
AhdI GACnn_n'nnGTC
Cuts at: 1170 1170
Size: 5009
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 865
Size: 32 4977
AlwNI CAG_nnn'CTG
Cuts at: 295 772 2314 3752 295
Size: 477 1542 1438 1552
Fragments arranged by size:
1552 1542 1438 477
ApaI G_GGCC'C
Cuts at: 794 794
Size: 5009
ApaLI G'TGCA_C
Cuts at: 3847 3847
Size: 5009
ApoI r'AATT_y
Cuts at: 1030 1303 2013 4362 4618 1030
Size: 273 710 2349 256 1421
Fragments arranged by size:
2349 1421 710 273 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5009
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
AvaI C'yCGr_G
Cuts at: 2029 2029
Size: 5009
AvrII C'CTAG_G
Cuts at: 1962 1962
Size: 5009
BamHI G'GATC_C
Cuts at: 2034 2034
Size: 5009
BanI G'GyrC_C
Cuts at: 2025 2439 2687 2722 2025
Size: 414 248 35 4312
Fragments arranged by size:
4312 414 248 35
BanII G_rGCy'C
Cuts at: 794 2023 3053 794
Size: 1229 1030 2750
Fragments arranged by size:
2750 1229 1030
BbeI G_GCGC'C
Cuts at: 2443 2691 2443
Size: 248 4761
BbsI GAAGACnn'nnnn_
Cuts at: 631 1466 631
Size: 835 4174
BbvCI CC'TCA_GC
Cuts at: 374 1333 374
Size: 959 4050
BciVI GTATCCnnnnn_n'
Cuts at: 105 2907 3963 5009 105
Size: 2802 1056 1046 105
Fragments arranged by size:
2802 1056 1046 105
BclI T'GATC_A
Cuts at: 2529 2529
Size: 5009
BglI GCCn_nnn'nGGC
Cuts at: 1594 1594
Size: 5009
BglII A'GATC_T
Cuts at: 2524 4561 2524
Size: 2037 2972
BlpI GC'TnA_GC
Cuts at: 2121 2121
Size: 5009
Bme1580I G_kGCm'C
Cuts at: 794 937 1637 2634 2727 3851 794
Size: 143 700 997 93 1124 1952
Fragments arranged by size:
1952 1124 997 700 143 93
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1840 2221 2302 2637 4781 1642
Size: 198 381 81 335 2144 1870
Fragments arranged by size:
2144 1870 381 335 198 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 442 1574 3376 442
Size: 1132 1802 2075
Fragments arranged by size:
2075 1802 1132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1735 925
Size: 810 4199
Bpu10I CC'TnA_GC
Cuts at: 374 1333 374
Size: 959 4050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 1984 2185 3064 3778 4076 1849
Size: 135 201 879 714 298 2782
Fragments arranged by size:
2782 879 714 298 201 135
BsaI GGTCTCn'nnnn_
Cuts at: 1077 4713 1077
Size: 3636 1373
BsaAI yAC'GTr
Cuts at: 334 2362 334
Size: 2028 2981
BsaBI GATnn'nnATC
Cuts at: 6 2523 4560 6
Size: 2517 2037 455
Fragments arranged by size:
2517 2037 455
BsaHI Gr'CG_yC
Cuts at: 2440 2688 3390 4886 2440
Size: 248 702 1496 2563
Fragments arranged by size:
2563 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2198 2719 3397 3808 3955 4229 2198
Size: 521 678 411 147 274 2978
Fragments arranged by size:
2978 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 865 895 865
Size: 30 4979
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 1321 478
Size: 843 4166
BseYI C'CCAG_C
Cuts at: 258 520 2434 3192 3857 4265 258
Size: 262 1914 758 665 408 1002
Fragments arranged by size:
1914 1002 758 665 408 262
BsiEI CG_ry'CG
Cuts at: 104 2551 2597 3827 104
Size: 2447 46 1230 1286
Fragments arranged by size:
2447 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 309 2023 2801 2991 3851 309
Size: 1714 778 190 860 1467
Fragments arranged by size:
1714 1467 860 778 190
BsmI GAATG_Cn'
Cuts at: 449 1112 1160 449
Size: 663 48 4298
Fragments arranged by size:
4298 663 48
Bsp1286I G_dGCh'C
Cuts at: 309 794 937 1637 2023 2634 2727 2801 2991
Size: 485 143 700 386 611 93 74 190
Cuts at: 2991 3053 3851 309
Size: 62 798 1467
Fragments arranged by size:
1467 798 700 611 485 386 190 143
93 74 62
BspHI T'CATG_A
Cuts at: 1535 1733 3441 1535
Size: 198 1708 3103
Fragments arranged by size:
3103 1708 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2059 2273 2575 2956 2059
Size: 214 302 381 4112
Fragments arranged by size:
4112 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3301 3355 4839 5004 50
Size: 3251 54 1484 165 55
Fragments arranged by size:
3251 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 522 2921 522
Size: 2399 2610
BsrFI r'CCGG_y
Cuts at: 3007 3188 3007
Size: 181 4828
BssSI C'ACGA_G
Cuts at: 3280 3988 3280
Size: 708 4301
BstAPI GCAn_nnn'nTGC
Cuts at: 153 2386 153
Size: 2233 2776
BstBI TT'CG_AA
Cuts at: 1386 3370 1386
Size: 1984 3025
BstXI CCAn_nnnn'nTGG
Cuts at: 1492 1492
Size: 5009
BstYI r'GATC_y
Cuts at: 1 273 1262 2034 2501 2524 2859 3105 3411
Size: 272 989 772 467 23 335 246 306
Cuts at: 3411 3423 3509 4561 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 989 772 467 449 335 306 272
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5009
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2959 3140 3303 2959
Size: 181 163 4665
Fragments arranged by size:
4665 181 163
BtsI GCAGTG_nn'
Cuts at: 2252 2252
Size: 5009
DraI TTT'AAA
Cuts at: 2008 2008
Size: 5009
DrdI GACnn_nn'nnGTC
Cuts at: 2715 4059 2715
Size: 1344 3665
EaeI y'GGCC_r
Cuts at: 2594 2768 3159 3186 4531 2594
Size: 174 391 27 1345 3072
Fragments arranged by size:
3072 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2594 2594
Size: 5009
EarI CTCTTCn'nnn_
Cuts at: 618 637 1409 3032 3242 618
Size: 19 772 1623 210 2385
Fragments arranged by size:
2385 1623 772 210 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3217 3959 4105 4819 3217
Size: 742 146 714 3407
Fragments arranged by size:
3407 742 714 146
EcoICRI GAG'CTC
Cuts at: 2021 2021
Size: 5009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 658 925 1069 1735 2834 3266 3619 4281
Size: 315 267 144 666 1099 432 353 662
Cuts at: 4281 343
Size: 1071
Fragments arranged by size:
1099 1071 666 662 432 353 315 267
144
EcoO109I rG'GnC_Cy
Cuts at: 1834 2148 1834
Size: 314 4695
EcoRI G'AATT_C
Cuts at: 2013 2013
Size: 5009
EcoRV GAT'ATC
Cuts at: 573 1350 573
Size: 777 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1399 1420 3028 1399
Size: 21 1608 3380
Fragments arranged by size:
3380 1608 21
FspI TGC'GCA
Cuts at: 2790 2790
Size: 5009
HaeII r_GCGC'y
Cuts at: 660 1698 2443 2691 3921 4692 660
Size: 1038 745 248 1230 771 977
Fragments arranged by size:
1230 1038 977 771 745 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 595 627 1156 1188 2851 2881 2883
Size: 32 106 32 529 32 1663 30 2
Cuts at: 2883 2913 457
Size: 30 2553
Fragments arranged by size:
2553 1663 529 106 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1678 2048 1678
Size: 370 4639
KasI G'GCGC_C
Cuts at: 2439 2687 2439
Size: 248 4761
KpnI G_GTAC'C
Cuts at: 2029 2029
Size: 5009
MluI A'CGCG_T
Cuts at: 4442 4442
Size: 5009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3768 3952 3768
Size: 184 4825
MscI TGG'CCA
Cuts at: 2770 2770
Size: 5009
MslI CAynn'nnrTG
Cuts at: 1204 1738 2998 3125 1204
Size: 534 1260 127 3088
Fragments arranged by size:
3088 1260 534 127
MspA1I CmG'CkG
Cuts at: 411 903 1097 2120 2434 2794 3578 3823 411
Size: 492 194 1023 314 360 784 245 1597
Fragments arranged by size:
1597 1023 784 492 360 314 245 194
NaeI GCC'GGC
Cuts at: 3190 3190
Size: 5009
NarI GG'CG_CC
Cuts at: 2440 2688 2440
Size: 248 4761
NcoI C'CATG_G
Cuts at: 107 107
Size: 5009
NgoMIV G'CCGG_C
Cuts at: 3188 3188
Size: 5009
NspI r_CATG'y
Cuts at: 331 1771 2062 2562 4165 331
Size: 1440 291 500 1603 1175
Fragments arranged by size:
1603 1440 1175 500 291
PciI A'CATG_T
Cuts at: 1767 4161 1767
Size: 2394 2615
PflMI CCAn_nnn'nTGG
Cuts at: 258 258
Size: 5009
PmeI GTTT'AAAC
Cuts at: 2008 2008
Size: 5009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 865 897 1887 1919 3455 3487 865
Size: 32 990 32 1536 32 2387
Fragments arranged by size:
2387 1536 990 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1834 1834
Size: 5009
PshAI GACnn'nnGTC
Cuts at: 1097 1097
Size: 5009
PspOMI G'GGCC_C
Cuts at: 790 790
Size: 5009
PstI C_TGCA'G
Cuts at: 729 2056 2741 729
Size: 1327 685 2997
Fragments arranged by size:
2997 1327 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5009
PvuII CAG'CTG
Cuts at: 411 903 2434 2794 411
Size: 492 1531 360 2626
Fragments arranged by size:
2626 1531 492 360
RsrII CG'GwC_CG
Cuts at: 3204 3204
Size: 5009
SacI G_AGCT'C
Cuts at: 2023 2023
Size: 5009
SalI G'TCGA_C
Cuts at: 2046 2046
Size: 5009
SapI GCTCTTCn'nnn_
Cuts at: 3032 3242 3032
Size: 210 4799
SbfI CC_TGCA'GG
Cuts at: 2056 2056
Size: 5009
ScaI AGT'ACT
Cuts at: 4526 4526
Size: 5009
SfcI C'TryA_G
Cuts at: 33 725 764 1362 2052 2737 3705 3896 33
Size: 692 39 598 690 685 968 191 1146
Fragments arranged by size:
1146 968 692 690 685 598 191 39
SfoI GGC'GCC
Cuts at: 2441 2689 2441
Size: 248 4761
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
SmaI CCC'GGG
Cuts at: 2031 2031
Size: 5009
SmlI C'TyrA_G
Cuts at: 1864 1999 2164 3079 3793 4055 1864
Size: 135 165 915 714 262 2818
Fragments arranged by size:
2818 915 714 262 165 135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5009
SphI G_CATG'C
Cuts at: 331 2062 2562 331
Size: 1731 500 2778
Fragments arranged by size:
2778 1731 500
StuI AGG'CCT
Cuts at: 1253 1253
Size: 5009
StyI C'CwwG_G
Cuts at: 107 868 1682 1962 2143 2497 107
Size: 761 814 280 181 354 2619
Fragments arranged by size:
2619 814 761 354 280 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2592 2725 3184 3393 2592
Size: 133 459 209 4208
Fragments arranged by size:
4208 459 209 133
TatI w'GTAC_w
Cuts at: 4524 4524
Size: 5009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1883 1915 4526 4558 1883
Size: 32 2611 32 2334
Fragments arranged by size:
2611 2334 32 32
Tth111I GACn'n_nGTC
Cuts at: 2806 2806
Size: 5009
XbaI T'CTAG_A
Cuts at: 2040 2040
Size: 5009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2504 2504
Size: 5009
XmaI C'CCGG_G
Cuts at: 2029 2029
Size: 5009
XmnI GAAnn'nnTTC
Cuts at: 628 1403 628
Size: 775 4234
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI