Restriction map for
pF1KA1480
(Circular) (Six-base) MAPSORT of: pF1KA1480.seq Check: 1985 from: 1 to: 5537
pF1KA1480 5537 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2022 2022
Size: 5537
AccI GT'mk_AC
Cuts at: 599 1360 2575 599
Size: 761 1215 3561
Fragments arranged by size:
3561 1215 761
Acc65I G'GTAC_C
Cuts at: 2553 2553
Size: 5537
AclI AA'CG_TT
Cuts at: 354 2153 5377 354
Size: 1799 3224 514
Fragments arranged by size:
3224 1799 514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3362 3794 4147 4809 3362
Size: 432 353 662 4090
Fragments arranged by size:
4090 662 432 353
AflIII A'CryG_T
Cuts at: 1895 2889 4689 4970 1895
Size: 994 1800 281 2462
Fragments arranged by size:
2462 1800 994 281
AleI CACnn'nnGTG
Cuts at: 445 1846 1924 2309 445
Size: 1401 78 385 3673
Fragments arranged by size:
3673 1401 385 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1070 1070
Size: 5537
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1708 1740 1708
Size: 32 5505
AlwNI CAG_nnn'CTG
Cuts at: 2842 4280 2842
Size: 1438 4099
ApaI G_GGCC'C
Cuts at: 2244 2296 2244
Size: 52 5485
ApaLI G'TGCA_C
Cuts at: 4375 4375
Size: 5537
ApoI r'AATT_y
Cuts at: 2541 4890 5146 2541
Size: 2349 256 2932
Fragments arranged by size:
2932 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5537
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
AvaI C'yCGr_G
Cuts at: 269 846 1781 2525 2557 269
Size: 577 935 744 32 3249
Fragments arranged by size:
3249 935 744 577 32
BamHI G'GATC_C
Cuts at: 1692 2562 1692
Size: 870 4667
BanI G'GyrC_C
Cuts at: 265 321 1869 2195 2553 2967 3215 3250 265
Size: 56 1548 326 358 414 248 35 2552
Fragments arranged by size:
2552 1548 414 358 326 248 56 35
BanII G_rGCy'C
Cuts at: 148 518 761 1445 2207 2244 2296 2551 3581
Size: 370 243 684 762 37 52 255 1030
Cuts at: 3581 148
Size: 2104
Fragments arranged by size:
2104 1030 762 684 370 255 243 52
37
BbeI G_GCGC'C
Cuts at: 2199 2971 3219 2199
Size: 772 248 4517
Fragments arranged by size:
4517 772 248
BbsI GAAGACnn'nnnn_
Cuts at: 530 1033 530
Size: 503 5034
BbvCI CC'TCA_GC
Cuts at: 205 2225 205
Size: 2020 3517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 639 660 673 694 784 818 1450 1484 639
Size: 21 13 21 90 34 632 34 4692
Fragments arranged by size:
4692 632 90 34 34 21 21 13
BciVI GTATCCnnnnn_n'
Cuts at: 1932 3435 4491 5537 1932
Size: 1503 1056 1046 1932
Fragments arranged by size:
1932 1503 1056 1046
BclI T'GATC_A
Cuts at: 3057 3057
Size: 5537
BglI GCCn_nnn'nGGC
Cuts at: 2131 2211 2131
Size: 80 5457
BglII A'GATC_T
Cuts at: 3052 5089 3052
Size: 2037 3500
BlpI GC'TnA_GC
Cuts at: 2649 2649
Size: 5537
Bme1580I G_kGCm'C
Cuts at: 270 326 427 452 1874 2244 2296 3162 3255
Size: 56 101 25 1422 370 52 866 93
Cuts at: 3255 4379 270
Size: 1124 1428
Fragments arranged by size:
1428 1422 1124 866 370 101 93 56
52 25
BmrI ACTGGGnnnn_n'
Cuts at: 278 412 1377 1697 2299 2749 2830 3165 5309
Size: 134 965 320 602 450 81 335 2144
Cuts at: 5309 278
Size: 506
Fragments arranged by size:
2144 965 602 506 450 335 320 134
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1807 3904 1807
Size: 2097 3440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 2007 2246 749
Size: 1258 239 4040
Fragments arranged by size:
4040 1258 239
Bpu10I CC'TnA_GC
Cuts at: 142 205 259 2225 142
Size: 63 54 1966 3454
Fragments arranged by size:
3454 1966 63 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2713 3592 4306 4604 1204
Size: 1509 879 714 298 2137
Fragments arranged by size:
2137 1509 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 812 1222 1336 1384 1778 5241 812
Size: 410 114 48 394 3463 1108
Fragments arranged by size:
3463 1108 410 394 114 48
BsaAI yAC'GTr
Cuts at: 1101 2890 1101
Size: 1789 3748
BsaBI GATnn'nnATC
Cuts at: 6 3051 5088 6
Size: 3045 2037 455
Fragments arranged by size:
3045 2037 455
BsaHI Gr'CG_yC
Cuts at: 2196 2968 3216 3918 5414 2196
Size: 772 248 702 1496 2319
Fragments arranged by size:
2319 1496 772 702 248
BsaWI w'CCGG_w
Cuts at: 395 1935 2726 3247 3925 4336 4483 4757 395
Size: 1540 791 521 678 411 147 274 1175
Fragments arranged by size:
1540 1175 791 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2500 2530 2500
Size: 30 5507
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 865 2281 2284 865
Size: 1416 3 4118
Fragments arranged by size:
4118 1416 3
BseYI C'CCAG_C
Cuts at: 225 785 947 2962 3720 4385 4793 225
Size: 560 162 2015 758 665 408 969
Fragments arranged by size:
2015 969 758 665 560 408 162
BsiEI CG_ry'CG
Cuts at: 104 344 3079 3125 4355 104
Size: 240 2735 46 1230 1286
Fragments arranged by size:
2735 1286 1230 240 46
BsiHKAI G_wGCw'C
Cuts at: 1993 2551 3329 3519 4379 1993
Size: 558 778 190 860 3151
Fragments arranged by size:
3151 860 778 558 190
BsmI GAATG_Cn'
Cuts at: 2037 2037
Size: 5537
BsmBI CGTCTCn'nnnn_
Cuts at: 1290 1290
Size: 5537
Bsp1286I G_dGCh'C
Cuts at: 148 270 326 427 452 518 761 1445 1874
Size: 122 56 101 25 66 243 684 429
Cuts at: 1874 1993 2207 2244 2296 2551 3162 3255 3329
Size: 119 214 37 52 255 611 93 74
Cuts at: 3329 3519 3581 4379 148
Size: 190 62 798 1306
Fragments arranged by size:
1306 798 684 611 429 255 243 214
190 122 119 101 93 74 66 62
56 52 37 25
BspEI T'CCGG_A
Cuts at: 395 1935 395
Size: 1540 3997
BspHI T'CATG_A
Cuts at: 1526 1658 2402 3969 1526
Size: 132 744 1567 3094
Fragments arranged by size:
3094 1567 744 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1895 2022 2587 2801 3103 3484 1895
Size: 127 565 214 302 381 3948
Fragments arranged by size:
3948 565 381 302 214 127
BsrBI CCG'CTC
Cuts at: 50 3829 3883 5367 5532 50
Size: 3779 54 1484 165 55
Fragments arranged by size:
3779 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 678 2350 2455 3449 678
Size: 1672 105 994 2766
Fragments arranged by size:
2766 1672 994 105
BsrFI r'CCGG_y
Cuts at: 465 1704 2317 3535 3716 465
Size: 1239 613 1218 181 2286
Fragments arranged by size:
2286 1239 1218 613 181
BssSI C'ACGA_G
Cuts at: 2090 2305 3808 4516 2090
Size: 215 1503 708 3111
Fragments arranged by size:
3111 1503 708 215
BstAPI GCAn_nnn'nTGC
Cuts at: 2457 2914 2457
Size: 457 5080
BstBI TT'CG_AA
Cuts at: 3898 3898
Size: 5537
BstXI CCAn_nnnn'nTGG
Cuts at: 987 1065 1296 1848 2127 2439 987
Size: 78 231 552 279 312 4085
Fragments arranged by size:
4085 552 312 279 231 78
BstYI r'GATC_y
Cuts at: 1 290 770 1151 1692 2562 3029 3052 3387
Size: 289 480 381 541 870 467 23 335
Cuts at: 3387 3633 3939 3951 4037 5089 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 870 541 480 467 449 381 335
306 289 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1361 1361
Size: 5537
Bsu36I CC'TnA_GG
Cuts at: 1247 1319 1247
Size: 72 5465
BtgI C'CryG_G
Cuts at: 155 606 1022 2126 155
Size: 451 416 1104 3566
Fragments arranged by size:
3566 1104 451 416
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2106 3487 3668 3831 2106
Size: 1381 181 163 3812
Fragments arranged by size:
3812 1381 181 163
BtsI GCAGTG_nn'
Cuts at: 277 476 1512 2345 2780 277
Size: 199 1036 833 435 3034
Fragments arranged by size:
3034 1036 833 435 199
DraI TTT'AAA
Cuts at: 2536 2536
Size: 5537
DraIII CAC_nnn'GTG
Cuts at: 185 185
Size: 5537
DrdI GACnn_nn'nnGTC
Cuts at: 1282 3243 4587 1282
Size: 1961 1344 2232
Fragments arranged by size:
2232 1961 1344
EaeI y'GGCC_r
Cuts at: 1999 3122 3296 3687 3714 5059 1999
Size: 1123 174 391 27 1345 2477
Fragments arranged by size:
2477 1345 1123 391 174 27
EagI C'GGCC_G
Cuts at: 3122 3122
Size: 5537
EarI CTCTTCn'nnn_
Cuts at: 1423 3560 3770 1423
Size: 2137 210 3190
Fragments arranged by size:
3190 2137 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3745 4487 4633 5347 3745
Size: 742 146 714 3935
Fragments arranged by size:
3935 742 714 146
EcoICRI GAG'CTC
Cuts at: 2549 2549
Size: 5537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 2007 2246 3362 3794 4147 4809 749
Size: 1258 239 1116 432 353 662 1477
Fragments arranged by size:
1477 1258 1116 662 432 353 239
EcoNI CCTnn'n_nnAGG
Cuts at: 1592 1592
Size: 5537
EcoO109I rG'GnC_Cy
Cuts at: 222 298 777 1597 2042 2132 2240 2241 2293
Size: 76 479 820 445 90 108 1 52
Cuts at: 2293 2676 222
Size: 383 3083
Fragments arranged by size:
3083 820 479 445 383 108 90 76
52 1
EcoRI G'AATT_C
Cuts at: 2541 2541
Size: 5537
EcoRV GAT'ATC
Cuts at: 492 492
Size: 5537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1869 3556 1869
Size: 1687 3850
FspI TGC'GCA
Cuts at: 3318 3318
Size: 5537
HaeII r_GCGC'y
Cuts at: 205 2199 2382 2971 3219 4449 5220 205
Size: 1994 183 589 248 1230 771 522
Fragments arranged by size:
1994 1230 771 589 522 248 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 905 937 1243 1275 1331 1363 1446 1478 1677
Size: 32 306 32 56 32 83 32 199
Cuts at: 1677 1709 3379 3409 3411 3441 905
Size: 32 1670 30 2 30 3001
Fragments arranged by size:
3001 1670 306 199 83 56 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 240 1203 2576 240
Size: 963 1373 3201
Fragments arranged by size:
3201 1373 963
KasI G'GCGC_C
Cuts at: 2195 2967 3215 2195
Size: 772 248 4517
Fragments arranged by size:
4517 772 248
KpnI G_GTAC'C
Cuts at: 2557 2557
Size: 5537
MluI A'CGCG_T
Cuts at: 4970 4970
Size: 5537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 684 4296 4480 684
Size: 3612 184 1741
Fragments arranged by size:
3612 1741 184
MscI TGG'CCA
Cuts at: 3298 3298
Size: 5537
MslI CAynn'nnrTG
Cuts at: 445 1559 1846 1924 2125 2309 2422 3526 3653
Size: 1114 287 78 201 184 113 1104 127
Cuts at: 3653 445
Size: 2329
Fragments arranged by size:
2329 1114 1104 287 201 184 127 113
78
MspA1I CmG'CkG
Cuts at: 445 785 947 2229 2648 2962 3322 4106 4351
Size: 340 162 1282 419 314 360 784 245
Cuts at: 4351 445
Size: 1631
Fragments arranged by size:
1631 1282 784 419 360 340 314 245
162
NaeI GCC'GGC
Cuts at: 3718 3718
Size: 5537
NarI GG'CG_CC
Cuts at: 2196 2968 3216 2196
Size: 772 248 4517
Fragments arranged by size:
4517 772 248
NgoMIV G'CCGG_C
Cuts at: 3716 3716
Size: 5537
NspI r_CATG'y
Cuts at: 1471 1899 2590 3090 4693 1471
Size: 428 691 500 1603 2315
Fragments arranged by size:
2315 1603 691 500 428
PasI CC'CwG_GG
Cuts at: 2037 2037
Size: 5537
PciI A'CATG_T
Cuts at: 1895 4689 1895
Size: 2794 2743
PflMI CCAn_nnn'nTGG
Cuts at: 315 1437 315
Size: 1122 4415
PfoI T'CCnGG_A
Cuts at: 509 569 2044 509
Size: 60 1475 4002
Fragments arranged by size:
4002 1475 60
PmeI GTTT'AAAC
Cuts at: 2536 2536
Size: 5537
PmlI CAC'GTG
Cuts at: 1101 1101
Size: 5537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 5505
PpuMI rG'GwC_Cy
Cuts at: 2042 2042
Size: 5537
PspOMI G'GGCC_C
Cuts at: 2240 2292 2240
Size: 52 5485
PstI C_TGCA'G
Cuts at: 2453 2584 3269 2453
Size: 131 685 4721
Fragments arranged by size:
4721 685 131
PvuI CG_AT'CG
Cuts at: 104 344 104
Size: 240 5297
PvuII CAG'CTG
Cuts at: 445 947 2962 3322 445
Size: 502 2015 360 2660
Fragments arranged by size:
2660 2015 502 360
RsrII CG'GwC_CG
Cuts at: 3732 3732
Size: 5537
SacI G_AGCT'C
Cuts at: 2551 2551
Size: 5537
SalI G'TCGA_C
Cuts at: 2574 2574
Size: 5537
SapI GCTCTTCn'nnn_
Cuts at: 3560 3770 3560
Size: 210 5327
SbfI CC_TGCA'GG
Cuts at: 2584 2584
Size: 5537
ScaI AGT'ACT
Cuts at: 726 5054 726
Size: 4328 1209
SfcI C'TryA_G
Cuts at: 33 2019 2449 2580 3265 4233 4424 33
Size: 1986 430 131 685 968 191 1146
Fragments arranged by size:
1986 1146 968 685 430 191 131
SfoI GGC'GCC
Cuts at: 2197 2969 3217 2197
Size: 772 248 4517
Fragments arranged by size:
4517 772 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
SmaI CCC'GGG
Cuts at: 2527 2559 2527
Size: 32 5505
SmlI C'TyrA_G
Cuts at: 1219 2692 3607 4321 4583 1219
Size: 1473 915 714 262 2173
Fragments arranged by size:
2173 1473 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5537
SphI G_CATG'C
Cuts at: 1471 2590 3090 1471
Size: 1119 500 3918
Fragments arranged by size:
3918 1119 500
SspI AAT'ATT
Cuts at: 801 801
Size: 5537
StyI C'CwwG_G
Cuts at: 743 1058 1736 1816 2671 3025 743
Size: 315 678 80 855 354 3255
Fragments arranged by size:
3255 855 678 354 315 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 246 777 1425 1960 2314 3120 3253 3712 3921
Size: 531 648 535 354 806 133 459 209
Cuts at: 3921 246
Size: 1862
Fragments arranged by size:
1862 806 648 535 531 459 354 209
133
TatI w'GTAC_w
Cuts at: 724 974 5052 724
Size: 250 4078 1209
Fragments arranged by size:
4078 1209 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 396 428 1894 1926 2500 2532 5054 5086 396
Size: 32 1466 32 574 32 2522 32 847
Fragments arranged by size:
2522 1466 847 574 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3334 3334
Size: 5537
XbaI T'CTAG_A
Cuts at: 2568 2568
Size: 5537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 291 380 1105 3032 291
Size: 89 725 1927 2796
Fragments arranged by size:
2796 1927 725 89
XmaI C'CCGG_G
Cuts at: 2525 2557 2525
Size: 32 5505
XmnI GAAnn'nnTTC
Cuts at: 355 355
Size: 5537
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BfrBI BmgBI BmtI BsgI BsiWI BsrGI BssHII BstEII
ClaI CspCI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI