Restriction map for
pF1KA1489
(Circular) (Six-base) MAPSORT of: pF1KA1489.seq Check: 3702 from: 1 to: 4982
pF1KA1489 4982 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2020 2020
Size: 4982
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 462 4822 462
Size: 4360 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 917 1111 1122 1478 1577 2807 3239 3592
Size: 398 194 11 356 99 1230 432 353
Cuts at: 3592 4254 519
Size: 662 1247
Fragments arranged by size:
1247 1230 662 432 398 356 353 194
99 11
AflIII A'CryG_T
Cuts at: 259 608 1815 2334 4134 4415 259
Size: 349 1207 519 1800 281 826
Fragments arranged by size:
1800 1207 826 519 349 281
AgeI A'CCGG_T
Cuts at: 1784 1784
Size: 4982
AhdI GACnn_n'nnGTC
Cuts at: 1788 1788
Size: 4982
AleI CACnn'nnGTG
Cuts at: 592 592
Size: 4982
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1388 1388
Size: 4982
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 568 600 568
Size: 32 4950
AlwNI CAG_nnn'CTG
Cuts at: 1600 2287 3725 1600
Size: 687 1438 2857
Fragments arranged by size:
2857 1438 687
ApaLI G'TGCA_C
Cuts at: 3820 3820
Size: 4982
ApoI r'AATT_y
Cuts at: 229 1517 1607 1701 1986 4335 4591 229
Size: 1288 90 94 285 2349 256 620
Fragments arranged by size:
2349 1288 620 285 256 94 90
AseI AT'TA_AT
Cuts at: 20 20
Size: 4982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 2002 2002
Size: 4982
BamHI G'GATC_C
Cuts at: 2007 2007
Size: 4982
BanI G'GyrC_C
Cuts at: 1998 2412 2660 2695 1998
Size: 414 248 35 4285
Fragments arranged by size:
4285 414 248 35
BanII G_rGCy'C
Cuts at: 1996 3026 1996
Size: 1030 3952
BbeI G_GCGC'C
Cuts at: 2416 2664 2416
Size: 248 4734
BbsI GAAGACnn'nnnn_
Cuts at: 740 1583 740
Size: 843 4139
BciVI GTATCCnnnnn_n'
Cuts at: 2880 3936 4982 2880
Size: 1056 1046 2880
Fragments arranged by size:
2880 1056 1046
BclI T'GATC_A
Cuts at: 2502 2502
Size: 4982
BfrBI ATG'CAT
Cuts at: 373 373
Size: 4982
BglII A'GATC_T
Cuts at: 2497 4534 2497
Size: 2037 2945
BlpI GC'TnA_GC
Cuts at: 2094 2094
Size: 4982
Bme1580I G_kGCm'C
Cuts at: 226 2607 2700 3824 226
Size: 2381 93 1124 1384
Fragments arranged by size:
2381 1384 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 732 2194 2275 2610 4754 732
Size: 1462 81 335 2144 960
Fragments arranged by size:
2144 1462 960 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 4982
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 2158 3037 3751 4049 101
Size: 2057 879 714 298 1034
Fragments arranged by size:
2057 1034 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1796 4686 1796
Size: 2890 2092
BsaAI yAC'GTr
Cuts at: 2335 2335
Size: 4982
BsaBI GATnn'nnATC
Cuts at: 6 2496 4533 6
Size: 2490 2037 455
Fragments arranged by size:
2490 2037 455
BsaHI Gr'CG_yC
Cuts at: 2413 2661 3363 4859 2413
Size: 248 702 1496 2536
Fragments arranged by size:
2536 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1784 2171 2692 3370 3781 3928 4202 1784
Size: 387 521 678 411 147 274 2564
Fragments arranged by size:
2564 678 521 411 387 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 568 598 1259 1289 1943 1973 568
Size: 30 661 30 654 30 3577
Fragments arranged by size:
3577 661 654 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1280 1280
Size: 4982
BseYI C'CCAG_C
Cuts at: 2407 3165 3830 4238 2407
Size: 758 665 408 3151
Fragments arranged by size:
3151 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 1394
Size: 4982
BsiEI CG_ry'CG
Cuts at: 104 2524 2570 3800 104
Size: 2420 46 1230 1286
Fragments arranged by size:
2420 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 581 1996 2774 2964 3824 581
Size: 1415 778 190 860 1739
Fragments arranged by size:
1739 1415 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 226 581 1996 2607 2700 2774 2964 3026 3824
Size: 355 1415 611 93 74 190 62 798
Cuts at: 3824 226
Size: 1384
Fragments arranged by size:
1415 1384 798 611 355 190 93 74
62
BspHI T'CATG_A
Cuts at: 1389 3414 1389
Size: 2025 2957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2032 2246 2548 2929 2032
Size: 214 302 381 4085
Fragments arranged by size:
4085 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3274 3328 4812 4977 50
Size: 3224 54 1484 165 55
Fragments arranged by size:
3224 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 398 1372 2894 398
Size: 974 1522 2486
Fragments arranged by size:
2486 1522 974
BsrFI r'CCGG_y
Cuts at: 1784 2980 3161 1784
Size: 1196 181 3605
Fragments arranged by size:
3605 1196 181
BsrGI T'GTAC_A
Cuts at: 321 321
Size: 4982
BssSI C'ACGA_G
Cuts at: 3253 3961 3253
Size: 708 4274
BstAPI GCAn_nnn'nTGC
Cuts at: 2359 2359
Size: 4982
BstBI TT'CG_AA
Cuts at: 3343 3343
Size: 4982
BstYI r'GATC_y
Cuts at: 1 2007 2474 2497 2832 3078 3384 3396 3482
Size: 2006 467 23 335 246 306 12 86
Cuts at: 3482 4534 1
Size: 1052 449
Fragments arranged by size:
2006 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 328 328
Size: 4982
BtgI C'CryG_G
Cuts at: 1891 1891
Size: 4982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2932 3113 3276 2932
Size: 181 163 4638
Fragments arranged by size:
4638 181 163
BtsI GCAGTG_nn'
Cuts at: 587 2225 587
Size: 1638 3344
DraI TTT'AAA
Cuts at: 665 1981 665
Size: 1316 3666
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2688 4032 1482
Size: 1206 1344 2432
Fragments arranged by size:
2432 1344 1206
EaeI y'GGCC_r
Cuts at: 1463 2567 2741 3132 3159 4504 1463
Size: 1104 174 391 27 1345 1941
Fragments arranged by size:
1941 1345 1104 391 174 27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4982
EarI CTCTTCn'nnn_
Cuts at: 538 963 1877 1928 3005 3215 538
Size: 425 914 51 1077 210 2305
Fragments arranged by size:
2305 1077 914 425 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3190 3932 4078 4792 3190
Size: 742 146 714 3380
Fragments arranged by size:
3380 742 714 146
EcoICRI GAG'CTC
Cuts at: 1994 1994
Size: 4982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 917 1111 1122 1478 1577 2807 3239 3592
Size: 398 194 11 356 99 1230 432 353
Cuts at: 3592 4254 519
Size: 662 1247
Fragments arranged by size:
1247 1230 662 432 398 356 353 194
99 11
EcoO109I rG'GnC_Cy
Cuts at: 1484 2121 1484
Size: 637 4345
EcoRI G'AATT_C
Cuts at: 229 1986 229
Size: 1757 3225
EcoRV GAT'ATC
Cuts at: 1059 1059
Size: 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3001 3001
Size: 4982
FspI TGC'GCA
Cuts at: 2763 2763
Size: 4982
HaeII r_GCGC'y
Cuts at: 2416 2664 3894 4665 2416
Size: 248 1230 771 2733
Fragments arranged by size:
2733 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1774 1806 2824 2854 2856 2886 1774
Size: 32 1018 30 2 30 3870
Fragments arranged by size:
3870 1018 32 30 30 2
HincII GTy'rAC
Cuts at: 2021 2021
Size: 4982
KasI G'GCGC_C
Cuts at: 2412 2660 2412
Size: 248 4734
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MfeI C'AATT_G
Cuts at: 1259 1259
Size: 4982
MluI A'CGCG_T
Cuts at: 608 4415 608
Size: 3807 1175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 3741 3925 140
Size: 3601 184 1197
Fragments arranged by size:
3601 1197 184
MscI TGG'CCA
Cuts at: 1465 2743 1465
Size: 1278 3704
MslI CAynn'nnrTG
Cuts at: 286 592 1402 1736 2971 3098 286
Size: 306 810 334 1235 127 2170
Fragments arranged by size:
2170 1235 810 334 306 127
MspA1I CmG'CkG
Cuts at: 621 1015 1498 2093 2407 2767 3551 3796 621
Size: 394 483 595 314 360 784 245 1807
Fragments arranged by size:
1807 784 595 483 394 360 314 245
NaeI GCC'GGC
Cuts at: 3163 3163
Size: 4982
NarI GG'CG_CC
Cuts at: 2413 2661 2413
Size: 248 4734
NcoI C'CATG_G
Cuts at: 1891 1891
Size: 4982
NgoMIV G'CCGG_C
Cuts at: 3161 3161
Size: 4982
NsiI A_TGCA'T
Cuts at: 375 375
Size: 4982
NspI r_CATG'y
Cuts at: 263 2035 2535 4138 263
Size: 1772 500 1603 1107
Fragments arranged by size:
1772 1603 1107 500
PacI TTA_AT'TAA
Cuts at: 482 482
Size: 4982
PciI A'CATG_T
Cuts at: 259 4134 259
Size: 3875 1107
PflMI CCAn_nnn'nTGG
Cuts at: 889 889
Size: 4982
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 568 600 3428 3460 568
Size: 32 2828 32 2090
Fragments arranged by size:
2828 2090 32 32
PpuMI rG'GwC_Cy
Cuts at: 1484 1484
Size: 4982
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1408 1440 1408
Size: 32 4950
PstI C_TGCA'G
Cuts at: 1503 2029 2714 1503
Size: 526 685 3771
Fragments arranged by size:
3771 685 526
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 621 1015 1498 2407 2767 621
Size: 394 483 909 360 2836
Fragments arranged by size:
2836 909 483 394 360
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 1996 1996
Size: 4982
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4982
SapI GCTCTTCn'nnn_
Cuts at: 3005 3215 3005
Size: 210 4772
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4982
ScaI AGT'ACT
Cuts at: 4499 4499
Size: 4982
SexAI A'CCwGG_T
Cuts at: 1183 1183
Size: 4982
SfcI C'TryA_G
Cuts at: 33 407 439 1499 1580 1973 2025 2710 3678
Size: 374 32 1060 81 393 52 685 968
Cuts at: 3678 3869 33
Size: 191 1146
Fragments arranged by size:
1146 1060 968 685 393 374 191 81
52 32
SfoI GGC'GCC
Cuts at: 2414 2662 2414
Size: 248 4734
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 2004 2004
Size: 4982
SmlI C'TyrA_G
Cuts at: 116 2137 3052 3766 4028 116
Size: 2021 915 714 262 1070
Fragments arranged by size:
2021 1070 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1477 67
Size: 1410 3572
SphI G_CATG'C
Cuts at: 2035 2535 2035
Size: 500 4482
SspI AAT'ATT
Cuts at: 475 475
Size: 4982
StyI C'CwwG_G
Cuts at: 1438 1891 2116 2470 1438
Size: 453 225 354 3950
Fragments arranged by size:
3950 453 354 225
SwaI ATTT'AAAT
Cuts at: 665 665
Size: 4982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 837 2565 2698 3157 3366 837
Size: 1728 133 459 209 2453
Fragments arranged by size:
2453 1728 459 209 133
TatI w'GTAC_w
Cuts at: 321 1424 1818 4497 321
Size: 1103 394 2679 806
Fragments arranged by size:
2679 1103 806 394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 4950
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4982
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2477 2477
Size: 4982
XmaI C'CCGG_G
Cuts at: 2002 2002
Size: 4982
XmnI GAAnn'nnTTC
Cuts at: 686 686
Size: 4982
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PacI PciI
PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI BpmI Bpu10I BsiWI
BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI XhoI ZraI