Restriction map for
pF1KA1492
(Circular) (Six-base) MAPSORT of: pF1KA1492.seq Check: 1807 from: 1 to: 5480
pF1KA1492 5480 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 544 2345 2518 544
Size: 1801 173 3506
Fragments arranged by size:
3506 1801 173
Acc65I G'GTAC_C
Cuts at: 867 2496 867
Size: 1629 3851
AclI AA'CG_TT
Cuts at: 5320 5320
Size: 5480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1669 1684 2400 3305 3737 4484 4752 1478
Size: 191 15 716 905 432 747 268 2206
Fragments arranged by size:
2206 905 747 716 432 268 191 15
AflIII A'CryG_T
Cuts at: 390 2832 3936 4913 390
Size: 2442 1104 977 957
Fragments arranged by size:
2442 1104 977 957
AgeI A'CCGG_T
Cuts at: 3868 3868
Size: 5480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 733 1660 733
Size: 927 4553
AlwNI CAG_nnn'CTG
Cuts at: 660 1252 1911 2785 4352 660
Size: 592 659 874 1567 1788
Fragments arranged by size:
1788 1567 874 659 592
ApaLI G'TGCA_C
Cuts at: 4250 4250
Size: 5480
ApoI r'AATT_y
Cuts at: 273 1234 1276 1397 1916 1981 2410 2484 4833
Size: 961 42 121 519 65 429 74 2349
Cuts at: 4833 5089 273
Size: 256 664
Fragments arranged by size:
2349 961 664 519 429 256 121 74
65 42
AseI AT'TA_AT
Cuts at: 20 2245 2299 20
Size: 2225 54 3201
Fragments arranged by size:
3201 2225 54
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
AvaI C'yCGr_G
Cuts at: 2500 2500
Size: 5480
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 350 383 350
Size: 33 5447
BamHI G'GATC_C
Cuts at: 2098 2505 2098
Size: 407 5073
BanI G'GyrC_C
Cuts at: 867 2496 2910 3158 3193 867
Size: 1629 414 248 35 3154
Fragments arranged by size:
3154 1629 414 248 35
BanII G_rGCy'C
Cuts at: 1010 1211 2494 3524 1010
Size: 201 1283 1030 2966
Fragments arranged by size:
2966 1283 1030 201
BbeI G_GCGC'C
Cuts at: 2914 3162 2914
Size: 248 5232
BbsI GAAGACnn'nnnn_
Cuts at: 308 308
Size: 5480
BbvCI CC'TCA_GC
Cuts at: 2139 2139
Size: 5480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5446
BciVI GTATCCnnnnn_n'
Cuts at: 917 935 941 3378 4139 5480 917
Size: 18 6 2437 761 1341 917
Fragments arranged by size:
2437 1341 917 761 18 6
BclI T'GATC_A
Cuts at: 3000 3000
Size: 5480
BfrBI ATG'CAT
Cuts at: 1481 1481
Size: 5480
BglII A'GATC_T
Cuts at: 1367 2995 5032 1367
Size: 1628 2037 1815
Fragments arranged by size:
2037 1815 1628
BlpI GC'TnA_GC
Cuts at: 2592 2592
Size: 5480
Bme1580I G_kGCm'C
Cuts at: 3105 3198 4254 3105
Size: 93 1056 4331
Fragments arranged by size:
4331 1056 93
BmgBI CAC'GTC
Cuts at: 1270 1270
Size: 5480
BmrI ACTGGGnnnn_n'
Cuts at: 160 1362 1576 1845 2692 2773 3108 5252 160
Size: 1202 214 269 847 81 335 2144 388
Fragments arranged by size:
2144 1202 847 388 335 269 214 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3847 3847
Size: 5480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 917 2342 587
Size: 330 1425 3725
Fragments arranged by size:
3725 1425 330
Bpu10I CC'TnA_GC
Cuts at: 2139 2139
Size: 5480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2656 3535 4027 4325 2656
Size: 879 492 298 3811
Fragments arranged by size:
3811 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1129 5184 1129
Size: 4055 1425
BsaAI yAC'GTr
Cuts at: 1184 2833 1184
Size: 1649 3831
BsaBI GATnn'nnATC
Cuts at: 6 1366 2994 5031 6
Size: 1360 1628 2037 455
Fragments arranged by size:
2037 1628 1360 455
BsaHI Gr'CG_yC
Cuts at: 2911 3159 3861 5357 2911
Size: 248 702 1496 3034
Fragments arranged by size:
3034 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2669 3190 3868 4142 4289 4700 2669
Size: 521 678 274 147 411 3449
Fragments arranged by size:
3449 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1198 1228 1993 2023 1198
Size: 30 765 30 4655
Fragments arranged by size:
4655 765 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1219 1219
Size: 5480
BseYI C'CCAG_C
Cuts at: 1611 2905 3663 4240 4736 1611
Size: 1294 758 577 496 2355
Fragments arranged by size:
2355 1294 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3022 3068 4276 104
Size: 2918 46 1208 1308
Fragments arranged by size:
2918 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1010 1414 2494 3272 3462 4254 1010
Size: 404 1080 778 190 792 2236
Fragments arranged by size:
2236 1080 792 778 404 190
BsmI GAATG_Cn'
Cuts at: 795 795
Size: 5480
BsmBI CGTCTCn'nnnn_
Cuts at: 1217 1217
Size: 5480
Bsp1286I G_dGCh'C
Cuts at: 1010 1211 1414 2494 3105 3198 3272 3462 3524
Size: 201 203 1080 611 93 74 190 62
Cuts at: 3524 4254 1010
Size: 730 2236
Fragments arranged by size:
2236 1080 730 611 203 201 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4700 4700
Size: 5480
BspHI T'CATG_A
Cuts at: 1496 4656 1496
Size: 3160 2320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 931 2331 2530 2744 3046 3427 931
Size: 1400 199 214 302 381 2984
Fragments arranged by size:
2984 1400 381 302 214 199
BsrBI CCG'CTC
Cuts at: 50 3772 3826 5310 5475 50
Size: 3722 54 1484 165 55
Fragments arranged by size:
3722 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 715 3392 715
Size: 2677 2803
BsrFI r'CCGG_y
Cuts at: 3478 3659 3868 3478
Size: 181 209 5090
Fragments arranged by size:
5090 209 181
BsrGI T'GTAC_A
Cuts at: 1442 1510 1442
Size: 68 5412
BssSI C'ACGA_G
Cuts at: 3751 4109 3751
Size: 358 5122
BstAPI GCAn_nnn'nTGC
Cuts at: 2857 2857
Size: 5480
BstBI TT'CG_AA
Cuts at: 3841 3841
Size: 5480
BstEII G'GTnAC_C
Cuts at: 818 818
Size: 5480
BstXI CCAn_nnnn'nTGG
Cuts at: 1083 1083
Size: 5480
BstYI r'GATC_y
Cuts at: 1 1367 2098 2505 2972 2995 3330 3576 4588
Size: 1366 731 407 467 23 335 246 1012
Cuts at: 4588 4674 4686 5032 1
Size: 86 12 346 449
Fragments arranged by size:
1366 1012 731 467 449 407 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 545 545
Size: 5480
Bsu36I CC'TnA_GG
Cuts at: 935 935
Size: 5480
BtgI C'CryG_G
Cuts at: 126 875 2102 126
Size: 749 1227 3504
Fragments arranged by size:
3504 1227 749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 789 3430 3611 3774 789
Size: 2641 181 163 2495
Fragments arranged by size:
2641 2495 181 163
BtsI GCAGTG_nn'
Cuts at: 2723 2723
Size: 5480
DraI TTT'AAA
Cuts at: 494 1029 2091 2479 494
Size: 535 1062 388 3495
Fragments arranged by size:
3495 1062 535 388
DrdI GACnn_nn'nnGTC
Cuts at: 3186 4044 3186
Size: 858 4622
EaeI y'GGCC_r
Cuts at: 3065 3239 3630 3657 5002 3065
Size: 174 391 27 1345 3543
Fragments arranged by size:
3543 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3065 3065
Size: 5480
EarI CTCTTCn'nnn_
Cuts at: 776 1778 2371 3503 3713 776
Size: 1002 593 1132 210 2543
Fragments arranged by size:
2543 1132 1002 593 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3688 3998 4144 5290 3688
Size: 310 146 1146 3878
Fragments arranged by size:
3878 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1008 2492 1008
Size: 1484 3996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 917 1478 1669 1684 2342 2400 3305 3737
Size: 330 561 191 15 658 58 905 432
Cuts at: 3737 4484 4752 587
Size: 747 268 1315
Fragments arranged by size:
1315 905 747 658 561 432 330 268
191 58 15
EcoNI CCTnn'n_nnAGG
Cuts at: 938 938
Size: 5480
EcoO109I rG'GnC_Cy
Cuts at: 1578 2619 1578
Size: 1041 4439
EcoRI G'AATT_C
Cuts at: 2484 2484
Size: 5480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 5480
FspI TGC'GCA
Cuts at: 112 3261 112
Size: 3149 2331
HaeII r_GCGC'y
Cuts at: 2914 3162 4184 5163 2914
Size: 248 1022 979 3231
Fragments arranged by size:
3231 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1159 1191 2370 2402 3322 3352 3354 3384 1159
Size: 32 1179 32 920 30 2 30 3255
Fragments arranged by size:
3255 1179 920 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2519 2519
Size: 5480
HindIII A'AGCT_T
Cuts at: 2083 2083
Size: 5480
KasI G'GCGC_C
Cuts at: 2910 3158 2910
Size: 248 5232
KpnI G_GTAC'C
Cuts at: 871 2500 871
Size: 1629 3851
MluI A'CGCG_T
Cuts at: 4913 4913
Size: 5480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2307 4151 4335 2307
Size: 1844 184 3452
Fragments arranged by size:
3452 1844 184
MscI TGG'CCA
Cuts at: 3241 3241
Size: 5480
MslI CAynn'nnrTG
Cuts at: 235 1291 1855 3469 3596 235
Size: 1056 564 1614 127 2119
Fragments arranged by size:
2119 1614 1056 564 127
MspA1I CmG'CkG
Cuts at: 128 660 1440 1809 1975 2591 2905 3265 4278
Size: 532 780 369 166 616 314 360 1013
Cuts at: 4278 4523 128
Size: 245 1085
Fragments arranged by size:
1085 1013 780 616 532 369 360 314
245 166
NaeI GCC'GGC
Cuts at: 3661 3661
Size: 5480
NarI GG'CG_CC
Cuts at: 2911 3159 2911
Size: 248 5232
NcoI C'CATG_G
Cuts at: 875 875
Size: 5480
NdeI CA'TA_TG
Cuts at: 513 555 513
Size: 42 5438
NgoMIV G'CCGG_C
Cuts at: 3659 3659
Size: 5480
NsiI A_TGCA'T
Cuts at: 1483 1483
Size: 5480
NspI r_CATG'y
Cuts at: 394 2533 3033 3940 394
Size: 2139 500 907 1934
Fragments arranged by size:
2139 1934 907 500
PciI A'CATG_T
Cuts at: 390 3936 390
Size: 3546 1934
PmeI GTTT'AAAC
Cuts at: 2479 2479
Size: 5480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4619 4651 4619
Size: 32 5448
PpuMI rG'GwC_Cy
Cuts at: 1578 1578
Size: 5480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1709 1741 1709
Size: 32 5448
PstI C_TGCA'G
Cuts at: 2527 3212 2527
Size: 685 4795
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5480
PvuII CAG'CTG
Cuts at: 660 1440 1809 1975 2905 3265 660
Size: 780 369 166 930 360 2875
Fragments arranged by size:
2875 930 780 369 360 166
RsrII CG'GwC_CG
Cuts at: 3675 3675
Size: 5480
SacI G_AGCT'C
Cuts at: 1010 2494 1010
Size: 1484 3996
SacII CC_GC'GG
Cuts at: 129 129
Size: 5480
SalI G'TCGA_C
Cuts at: 2517 2517
Size: 5480
SanDI GG'GwC_CC
Cuts at: 1578 1578
Size: 5480
SapI GCTCTTCn'nnn_
Cuts at: 1778 2371 3503 3713 1778
Size: 593 1132 210 3545
Fragments arranged by size:
3545 1132 593 210
SbfI CC_TGCA'GG
Cuts at: 2527 2527
Size: 5480
ScaI AGT'ACT
Cuts at: 4997 4997
Size: 5480
SfcI C'TryA_G
Cuts at: 33 1142 2394 2523 3208 4201 4392 33
Size: 1109 1252 129 685 993 191 1121
Fragments arranged by size:
1252 1121 1109 993 685 191 129
SfoI GGC'GCC
Cuts at: 2912 3160 2912
Size: 248 5232
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5480
SmaI CCC'GGG
Cuts at: 2502 2502
Size: 5480
SmlI C'TyrA_G
Cuts at: 2635 3550 4042 4304 2635
Size: 915 492 262 3811
Fragments arranged by size:
3811 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5480
SphI G_CATG'C
Cuts at: 2533 3033 2533
Size: 500 4980
SspI AAT'ATT
Cuts at: 1621 1621
Size: 5480
StyI C'CwwG_G
Cuts at: 648 875 1573 2160 2614 2968 648
Size: 227 698 587 454 354 3160
Fragments arranged by size:
3160 698 587 454 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1772 3063 3196 3655 3864 1772
Size: 1291 133 459 209 3388
Fragments arranged by size:
3388 1291 459 209 133
TatI w'GTAC_w
Cuts at: 1442 1510 4995 1442
Size: 68 3485 1927
Fragments arranged by size:
3485 1927 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4997 5029 4997
Size: 32 5448
Tth111I GACn'n_nGTC
Cuts at: 619 3277 619
Size: 2658 2822
XbaI T'CTAG_A
Cuts at: 1219 2511 1219
Size: 1292 4188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 176 294 344 432 1749 2975 114
Size: 62 118 50 88 1317 1226 2619
Fragments arranged by size:
2619 1317 1226 118 88 62 50
XmaI C'CCGG_G
Cuts at: 2500 2500
Size: 5480
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BglI BmtI BsgI BsiWI BssHII ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI