Restriction map for
pF1KA1496
(Circular) (Six-base) MAPSORT of: pF1KA1496.seq Check: 2409 from: 1 to: 6197
pF1KA1496 6197 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1362 1549 1362
Size: 187 6010
AccI GT'mk_AC
Cuts at: 3235 3235
Size: 6197
Acc65I G'GTAC_C
Cuts at: 3213 3213
Size: 6197
AclI AA'CG_TT
Cuts at: 6037 6037
Size: 6197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 2213 2243 2303 2734 2966 3101 4022 4454
Size: 1774 30 60 431 232 135 921 432
Cuts at: 4454 4807 5469 439
Size: 353 662 1167
Fragments arranged by size:
1774 1167 921 662 432 431 353 232
135 60 30
AflIII A'CryG_T
Cuts at: 691 3549 5349 5630 691
Size: 2858 1800 281 1258
Fragments arranged by size:
2858 1800 1258 281
AhdI GACnn_n'nnGTC
Cuts at: 2942 2942
Size: 6197
AleI CACnn'nnGTG
Cuts at: 694 2050 2860 694
Size: 1356 810 4031
Fragments arranged by size:
4031 1356 810
AlwNI CAG_nnn'CTG
Cuts at: 505 2056 2089 2281 3502 4940 505
Size: 1551 33 192 1221 1438 1762
Fragments arranged by size:
1762 1551 1438 1221 192 33
ApaI G_GGCC'C
Cuts at: 1728 1728
Size: 6197
ApaLI G'TGCA_C
Cuts at: 5035 5035
Size: 6197
ApoI r'AATT_y
Cuts at: 417 474 1008 1472 2122 3201 5550 5806 417
Size: 57 534 464 650 1079 2349 256 808
Fragments arranged by size:
2349 1079 808 650 534 464 256 57
AseI AT'TA_AT
Cuts at: 20 368 884 20
Size: 348 516 5333
Fragments arranged by size:
5333 516 348
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6197
AvaI C'yCGr_G
Cuts at: 722 3217 722
Size: 2495 3702
BamHI G'GATC_C
Cuts at: 2271 2351 3109 3222 2271
Size: 80 758 113 5246
Fragments arranged by size:
5246 758 113 80
BanI G'GyrC_C
Cuts at: 644 1213 1620 3213 3627 3875 3910 644
Size: 569 407 1593 414 248 35 2931
Fragments arranged by size:
2931 1593 569 414 407 248 35
BanII G_rGCy'C
Cuts at: 736 1310 1728 3211 4241 736
Size: 574 418 1483 1030 2692
Fragments arranged by size:
2692 1483 1030 574 418
BbeI G_GCGC'C
Cuts at: 3631 3879 3631
Size: 248 5949
BbsI GAAGACnn'nnnn_
Cuts at: 2080 2080
Size: 6197
BciVI GTATCCnnnnn_n'
Cuts at: 381 4095 5151 6197 381
Size: 3714 1056 1046 381
Fragments arranged by size:
3714 1056 1046 381
BclI T'GATC_A
Cuts at: 1796 3717 1796
Size: 1921 4276
BfrBI ATG'CAT
Cuts at: 998 998
Size: 6197
BglII A'GATC_T
Cuts at: 3712 5749 3712
Size: 2037 4160
BlpI GC'TnA_GC
Cuts at: 1304 3309 1304
Size: 2005 4192
Bme1580I G_kGCm'C
Cuts at: 647 1218 1425 1728 3822 3915 5039 647
Size: 571 207 303 2094 93 1124 1805
Fragments arranged by size:
2094 1805 1124 571 303 207 93
BmrI ACTGGGnnnn_n'
Cuts at: 1981 2473 2623 2810 3409 3490 3825 5969 1981
Size: 492 150 187 599 81 335 2144 2209
Fragments arranged by size:
2209 2144 599 492 335 187 150 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1057 4564 1057
Size: 3507 2690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 1313 578
Size: 735 5462
Bpu10I CC'TnA_GC
Cuts at: 156 156
Size: 6197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3373 4252 4966 5264 2513
Size: 860 879 714 298 3446
Fragments arranged by size:
3446 879 860 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5901 5901
Size: 6197
BsaAI yAC'GTr
Cuts at: 3550 3550
Size: 6197
BsaBI GATnn'nnATC
Cuts at: 6 3711 5748 6
Size: 3705 2037 455
Fragments arranged by size:
3705 2037 455
BsaHI Gr'CG_yC
Cuts at: 1038 3628 3876 4578 6074 1038
Size: 2590 248 702 1496 1161
Fragments arranged by size:
2590 1496 1161 702 248
BsaWI w'CCGG_w
Cuts at: 3386 3907 4585 4996 5143 5417 3386
Size: 521 678 411 147 274 4166
Fragments arranged by size:
4166 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 882 912 2887 2917 882
Size: 30 1975 30 4162
Fragments arranged by size:
4162 1975 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 507 1204 2249 507
Size: 697 1045 4455
Fragments arranged by size:
4455 1045 697
BseYI C'CCAG_C
Cuts at: 202 729 2767 3622 4380 5045 5453 202
Size: 527 2038 855 758 665 408 946
Fragments arranged by size:
2038 946 855 758 665 527 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 1477 1390
Size: 87 6110
BsiEI CG_ry'CG
Cuts at: 104 3739 3785 5015 104
Size: 3635 46 1230 1286
Fragments arranged by size:
3635 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1310 3211 3989 4179 5039 1310
Size: 1901 778 190 860 2468
Fragments arranged by size:
2468 1901 860 778 190
BsmI GAATG_Cn'
Cuts at: 1000 1126 2850 1000
Size: 126 1724 4347
Fragments arranged by size:
4347 1724 126
BsmBI CGTCTCn'nnnn_
Cuts at: 1045 1045
Size: 6197
Bsp1286I G_dGCh'C
Cuts at: 647 736 1218 1310 1425 1728 3211 3822 3915
Size: 89 482 92 115 303 1483 611 93
Cuts at: 3915 3989 4179 4241 5039 647
Size: 74 190 62 798 1805
Fragments arranged by size:
1805 1483 798 611 482 303 190 115
93 92 89 74 62
BspHI T'CATG_A
Cuts at: 4629 4629
Size: 6197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1362 1549 3247 3461 3763 4144 973
Size: 389 187 1698 214 302 381 3026
Fragments arranged by size:
3026 1698 389 381 302 214 187
BsrBI CCG'CTC
Cuts at: 50 4489 4543 6027 6192 50
Size: 4439 54 1484 165 55
Fragments arranged by size:
4439 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 998 2607 4109 998
Size: 1609 1502 3086
Fragments arranged by size:
3086 1609 1502
BsrFI r'CCGG_y
Cuts at: 2246 2761 4195 4376 2246
Size: 515 1434 181 4067
Fragments arranged by size:
4067 1434 515 181
BssSI C'ACGA_G
Cuts at: 1013 4468 5176 1013
Size: 3455 708 2034
Fragments arranged by size:
3455 2034 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3574 3574
Size: 6197
BstBI TT'CG_AA
Cuts at: 4558 4558
Size: 6197
BstEII G'GTnAC_C
Cuts at: 2340 2340
Size: 6197
BstYI r'GATC_y
Cuts at: 1 578 1750 2271 2351 3109 3222 3689 3712
Size: 577 1172 521 80 758 113 467 23
Cuts at: 3712 4047 4293 4599 4611 4697 5749 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1172 1052 758 577 521 467 449 335
306 246 113 86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 2057 2057
Size: 6197
BtgI C'CryG_G
Cuts at: 118 1097 2027 2107 2891 118
Size: 979 930 80 784 3424
Fragments arranged by size:
3424 979 930 784 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4147 4328 4491 4147
Size: 181 163 5853
Fragments arranged by size:
5853 181 163
BtsI GCAGTG_nn'
Cuts at: 3440 3440
Size: 6197
ClaI AT'CG_AT
Cuts at: 2066 2066
Size: 6197
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2076 2111 2076
Size: 35 6162
DraI TTT'AAA
Cuts at: 480 3196 480
Size: 2716 3481
DrdI GACnn_nn'nnGTC
Cuts at: 3903 5247 3903
Size: 1344 4853
EaeI y'GGCC_r
Cuts at: 3782 3956 4347 4374 5719 3782
Size: 174 391 27 1345 4260
Fragments arranged by size:
4260 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3782 3782
Size: 6197
EarI CTCTTCn'nnn_
Cuts at: 821 1434 2189 2501 2710 4220 4430 821
Size: 613 755 312 209 1510 210 2588
Fragments arranged by size:
2588 1510 755 613 312 210 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2255 2317 4405 5147 5293 6007 2255
Size: 62 2088 742 146 714 2445
Fragments arranged by size:
2445 2088 742 714 146 62
EcoICRI GAG'CTC
Cuts at: 1308 3209 1308
Size: 1901 4296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 578 1313 2213 2243 2303 2734 2966 3101
Size: 139 735 900 30 60 431 232 135
Cuts at: 3101 4022 4454 4807 5469 439
Size: 921 432 353 662 1167
Fragments arranged by size:
1167 921 900 735 662 432 431 353
232 139 135 60 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1416 1416
Size: 6197
EcoO109I rG'GnC_Cy
Cuts at: 181 1724 1725 2709 3336 181
Size: 1543 1 984 627 3042
Fragments arranged by size:
3042 1543 984 627 1
EcoRI G'AATT_C
Cuts at: 1008 3201 1008
Size: 2193 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 469 1493 1631 2884 4216 469
Size: 1024 138 1253 1332 2450
Fragments arranged by size:
2450 1332 1253 1024 138
FspI TGC'GCA
Cuts at: 3978 3978
Size: 6197
HaeII r_GCGC'y
Cuts at: 2768 2783 3631 3879 5109 5880 2768
Size: 15 848 248 1230 771 3085
Fragments arranged by size:
3085 1230 848 771 248 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2644 2676 2887 2919 2928 2960 4039 4069 4071
Size: 32 211 32 9 32 1079 30 2
Cuts at: 4071 4101 2644
Size: 30 4740
Fragments arranged by size:
4740 1079 211 32 32 32 30 30
9 2
HincII GTy'rAC
Cuts at: 3236 3236
Size: 6197
KasI G'GCGC_C
Cuts at: 3627 3875 3627
Size: 248 5949
KpnI G_GTAC'C
Cuts at: 3217 3217
Size: 6197
MfeI C'AATT_G
Cuts at: 431 431
Size: 6197
MluI A'CGCG_T
Cuts at: 5630 5630
Size: 6197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 598 763 993 4956 5140 598
Size: 165 230 3963 184 1655
Fragments arranged by size:
3963 1655 230 184 165
MscI TGG'CCA
Cuts at: 3958 3958
Size: 6197
MslI CAynn'nnrTG
Cuts at: 694 1543 2050 2860 4186 4313 694
Size: 849 507 810 1326 127 2578
Fragments arranged by size:
2578 1326 849 810 507 127
MspA1I CmG'CkG
Cuts at: 297 1689 1812 2986 3308 3622 3982 4766 5011
Size: 1392 123 1174 322 314 360 784 245
Cuts at: 5011 297
Size: 1483
Fragments arranged by size:
1483 1392 1174 784 360 322 314 245
123
NaeI GCC'GGC
Cuts at: 2763 4378 2763
Size: 1615 4582
NarI GG'CG_CC
Cuts at: 3628 3876 3628
Size: 248 5949
NcoI C'CATG_G
Cuts at: 118 2107 2891 118
Size: 1989 784 3424
Fragments arranged by size:
3424 1989 784
NdeI CA'TA_TG
Cuts at: 570 2427 570
Size: 1857 4340
NgoMIV G'CCGG_C
Cuts at: 2761 4376 2761
Size: 1615 4582
NsiI A_TGCA'T
Cuts at: 1000 1000
Size: 6197
NspI r_CATG'y
Cuts at: 695 1257 3250 3750 5353 695
Size: 562 1993 500 1603 1539
Fragments arranged by size:
1993 1603 1539 562 500
PciI A'CATG_T
Cuts at: 691 5349 691
Size: 4658 1539
PflMI CCAn_nnn'nTGG
Cuts at: 556 2361 2588 556
Size: 1805 227 4165
Fragments arranged by size:
4165 1805 227
PfoI T'CCnGG_A
Cuts at: 1432 1432
Size: 6197
PmeI GTTT'AAAC
Cuts at: 3196 3196
Size: 6197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 4643
Size: 32 6165
PsiI TTA'TAA
Cuts at: 338 2450 338
Size: 2112 4085
PspOMI G'GGCC_C
Cuts at: 1724 1724
Size: 6197
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2153 2185 2153
Size: 32 6165
PstI C_TGCA'G
Cuts at: 2502 3244 3929 2502
Size: 742 685 4770
Fragments arranged by size:
4770 742 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6197
PvuII CAG'CTG
Cuts at: 297 1812 2986 3622 3982 297
Size: 1515 1174 636 360 2512
Fragments arranged by size:
2512 1515 1174 636 360
RsrII CG'GwC_CG
Cuts at: 4392 4392
Size: 6197
SacI G_AGCT'C
Cuts at: 1310 3211 1310
Size: 1901 4296
SalI G'TCGA_C
Cuts at: 3234 3234
Size: 6197
SapI GCTCTTCn'nnn_
Cuts at: 2501 2710 4220 4430 2501
Size: 209 1510 210 4268
Fragments arranged by size:
4268 1510 210 209
SbfI CC_TGCA'GG
Cuts at: 3244 3244
Size: 6197
ScaI AGT'ACT
Cuts at: 2963 3164 5714 2963
Size: 201 2550 3446
Fragments arranged by size:
3446 2550 201
SexAI A'CCwGG_T
Cuts at: 1708 1708
Size: 6197
SfcI C'TryA_G
Cuts at: 33 2090 2290 2498 2513 2931 3158 3240 3925
Size: 2057 200 208 15 418 227 82 685
Cuts at: 3925 4893 5084 33
Size: 968 191 1146
Fragments arranged by size:
2057 1146 968 685 418 227 208 200
191 82 15
SfoI GGC'GCC
Cuts at: 3629 3877 3629
Size: 248 5949
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6197
SmaI CCC'GGG
Cuts at: 3219 3219
Size: 6197
SmlI C'TyrA_G
Cuts at: 2528 3352 4267 4981 5243 2528
Size: 824 915 714 262 3482
Fragments arranged by size:
3482 915 824 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6197
SphI G_CATG'C
Cuts at: 3250 3750 3250
Size: 500 5697
StyI C'CwwG_G
Cuts at: 118 861 1417 2107 2587 2891 3331 3685 118
Size: 743 556 690 480 304 440 354 2630
Fragments arranged by size:
2630 743 690 556 480 440 354 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 763 2100 2431 3780 3913 4372 4581 763
Size: 1337 331 1349 133 459 209 2379
Fragments arranged by size:
2379 1349 1337 459 331 209 133
TatI w'GTAC_w
Cuts at: 496 2961 3162 5712 496
Size: 2465 201 2550 981
Fragments arranged by size:
2550 2465 981 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 344 376 882 914 927 959 2628 2660 3048
Size: 32 506 32 13 32 1669 32 388
Cuts at: 3048 3080 5714 5746 344
Size: 32 2634 32 795
Fragments arranged by size:
2634 1669 795 506 388 32 32 32
32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 3994 3994
Size: 6197
XbaI T'CTAG_A
Cuts at: 3228 3228
Size: 6197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3692 3692
Size: 6197
XmaI C'CCGG_G
Cuts at: 3217 3217
Size: 6197
XmnI GAAnn'nnTTC
Cuts at: 815 815
Size: 6197
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsiWI BspEI
BsrGI BssHII BstXI BstZ17I DraIII EcoRV FseI FspAI
HindIII HpaI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI