Restriction map for
pF1KA1503
(Circular) (Six-base) MAPSORT of: pF1KA1503.seq Check: 8267 from: 1 to: 5483
pF1KA1503 5483 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 524 2521 524
Size: 1997 3486
Acc65I G'GTAC_C
Cuts at: 2499 2499
Size: 5483
AclI AA'CG_TT
Cuts at: 5323 5323
Size: 5483
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 1153 3308 3740 4487 4755 499
Size: 654 2155 432 747 268 1227
Fragments arranged by size:
2155 1227 747 654 432 268
AflIII A'CryG_T
Cuts at: 2835 3939 4916 2835
Size: 1104 977 3402
Fragments arranged by size:
3402 1104 977
AgeI A'CCGG_T
Cuts at: 3871 3871
Size: 5483
AhdI GACnn_n'nnGTC
Cuts at: 1919 1919
Size: 5483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 233 233
Size: 5483
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 928 960 928
Size: 32 5451
AlwNI CAG_nnn'CTG
Cuts at: 1527 2368 2788 4355 1527
Size: 841 420 1567 2655
Fragments arranged by size:
2655 1567 841 420
ApaI G_GGCC'C
Cuts at: 604 604
Size: 5483
ApaLI G'TGCA_C
Cuts at: 1759 1849 4253 1759
Size: 90 2404 2989
Fragments arranged by size:
2989 2404 90
ApoI r'AATT_y
Cuts at: 918 2068 2487 4836 5092 918
Size: 1150 419 2349 256 1309
Fragments arranged by size:
2349 1309 1150 419 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5483
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5483
AvaI C'yCGr_G
Cuts at: 185 322 353 1247 2458 2503 185
Size: 137 31 894 1211 45 3165
Fragments arranged by size:
3165 1211 894 137 45 31
BamHI G'GATC_C
Cuts at: 2508 2508
Size: 5483
BanI G'GyrC_C
Cuts at: 1053 1348 2499 2913 3161 3196 1053
Size: 295 1151 414 248 35 3340
Fragments arranged by size:
3340 1151 414 295 248 35
BanII G_rGCy'C
Cuts at: 257 323 604 1724 2324 2459 2497 3527 257
Size: 66 281 1120 600 135 38 1030 2213
Fragments arranged by size:
2213 1120 1030 600 281 135 66 38
BbeI G_GCGC'C
Cuts at: 1057 2917 3165 1057
Size: 1860 248 3375
Fragments arranged by size:
3375 1860 248
BbsI GAAGACnn'nnnn_
Cuts at: 1478 1478
Size: 5483
BbvCI CC'TCA_GC
Cuts at: 1536 1536
Size: 5483
BciVI GTATCCnnnnn_n'
Cuts at: 1450 1944 3381 4142 5483 1450
Size: 494 1437 761 1341 1450
Fragments arranged by size:
1450 1437 1341 761 494
BclI T'GATC_A
Cuts at: 1199 1529 3003 1199
Size: 330 1474 3679
Fragments arranged by size:
3679 1474 330
BfrBI ATG'CAT
Cuts at: 962 962
Size: 5483
BglI GCCn_nnn'nGGC
Cuts at: 187 2463 187
Size: 2276 3207
BglII A'GATC_T
Cuts at: 653 2998 5035 653
Size: 2345 2037 1101
Fragments arranged by size:
2345 2037 1101
BlpI GC'TnA_GC
Cuts at: 251 2595 251
Size: 2344 3139
Bme1580I G_kGCm'C
Cuts at: 604 761 894 1353 1763 1853 3108 3201 4257
Size: 157 133 459 410 90 1255 93 1056
Cuts at: 4257 604
Size: 1830
Fragments arranged by size:
1830 1255 1056 459 410 157 133 93
90
BmgBI CAC'GTC
Cuts at: 1560 1560
Size: 5483
BmrI ACTGGGnnnn_n'
Cuts at: 1728 2356 2695 2776 3111 5255 1728
Size: 628 339 81 335 2144 1956
Fragments arranged by size:
2144 1956 628 339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 5483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 952 1402 1528 1738 772
Size: 180 450 126 210 4517
Fragments arranged by size:
4517 450 210 180 126
Bpu10I CC'TnA_GC
Cuts at: 272 1193 1536 2453 272
Size: 921 343 917 3302
Fragments arranged by size:
3302 921 917 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 2338 2659 3538 4030 4328 278
Size: 2060 321 879 492 298 1433
Fragments arranged by size:
2060 1433 879 492 321 298
BsaI GGTCTCn'nnnn_
Cuts at: 838 2421 5187 838
Size: 1583 2766 1134
Fragments arranged by size:
2766 1583 1134
BsaAI yAC'GTr
Cuts at: 1728 2836 1728
Size: 1108 4375
BsaBI GATnn'nnATC
Cuts at: 6 2997 5034 6
Size: 2991 2037 455
Fragments arranged by size:
2991 2037 455
BsaHI Gr'CG_yC
Cuts at: 1054 1773 2914 3162 3864 5360 1054
Size: 719 1141 248 702 1496 1177
Fragments arranged by size:
1496 1177 1141 719 702 248
BsaWI w'CCGG_w
Cuts at: 1741 2672 3193 3871 4145 4292 4703 1741
Size: 931 521 678 274 147 411 2521
Fragments arranged by size:
2521 931 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 928 958 928
Size: 30 5453
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 949 2081 280
Size: 669 1132 3682
Fragments arranged by size:
3682 1132 669
BseYI C'CCAG_C
Cuts at: 489 1307 1754 2283 2299 2390 2908 3666 4243
Size: 818 447 529 16 91 518 758 577
Cuts at: 4243 4739 489
Size: 496 1233
Fragments arranged by size:
1233 818 758 577 529 518 496 447
91 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 694 1012 1396 601
Size: 93 318 384 4688
Fragments arranged by size:
4688 384 318 93
BsiEI CG_ry'CG
Cuts at: 104 3025 3071 4279 104
Size: 2921 46 1208 1308
Fragments arranged by size:
2921 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 257 1763 1812 1853 2497 3275 3465 4257 257
Size: 1506 49 41 644 778 190 792 1483
Fragments arranged by size:
1506 1483 792 778 644 190 49 41
BsmI GAATG_Cn'
Cuts at: 222 1937 222
Size: 1715 3768
Bsp1286I G_dGCh'C
Cuts at: 257 323 604 761 894 1353 1724 1763 1812
Size: 66 281 157 133 459 371 39 49
Cuts at: 1812 1853 2324 2459 2497 3108 3201 3275 3465
Size: 41 471 135 38 611 93 74 190
Cuts at: 3465 3527 4257 257
Size: 62 730 1483
Fragments arranged by size:
1483 730 611 471 459 371 281 190
157 135 133 93 74 66 62 49
41 39 38
BspEI T'CCGG_A
Cuts at: 1741 4703 1741
Size: 2962 2521
BspHI T'CATG_A
Cuts at: 1160 1790 4659 1160
Size: 630 2869 1984
Fragments arranged by size:
2869 1984 630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1804 2466 2533 2747 3049 3430 1804
Size: 662 67 214 302 381 3857
Fragments arranged by size:
3857 662 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 3775 3829 5313 5478 50
Size: 3725 54 1484 165 55
Fragments arranged by size:
3725 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1957 3395 1957
Size: 1438 4045
BsrFI r'CCGG_y
Cuts at: 3481 3662 3871 3481
Size: 181 209 5093
Fragments arranged by size:
5093 209 181
BsrGI T'GTAC_A
Cuts at: 501 501
Size: 5483
BssHII G'CGCG_C
Cuts at: 1750 1750
Size: 5483
BssSI C'ACGA_G
Cuts at: 3754 4112 3754
Size: 358 5125
BstAPI GCAn_nnn'nTGC
Cuts at: 959 1419 1572 2860 959
Size: 460 153 1288 3582
Fragments arranged by size:
3582 1288 460 153
BstBI TT'CG_AA
Cuts at: 1893 3844 1893
Size: 1951 3532
BstXI CCAn_nnnn'nTGG
Cuts at: 2291 2291
Size: 5483
BstYI r'GATC_y
Cuts at: 1 653 1079 1647 2508 2975 2998 3333 3579
Size: 652 426 568 861 467 23 335 246
Cuts at: 3579 4591 4677 4689 5035 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 861 652 568 467 449 426 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 277 277
Size: 5483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2297 3433 3614 3777 2297
Size: 1136 181 163 4003
Fragments arranged by size:
4003 1136 181 163
BtsI GCAGTG_nn'
Cuts at: 389 2726 389
Size: 2337 3146
DraI TTT'AAA
Cuts at: 2482 2482
Size: 5483
DraIII CAC_nnn'GTG
Cuts at: 304 1572 304
Size: 1268 4215
DrdI GACnn_nn'nnGTC
Cuts at: 1122 3189 4047 1122
Size: 2067 858 2558
Fragments arranged by size:
2558 2067 858
EaeI y'GGCC_r
Cuts at: 144 197 1708 2253 3068 3242 3633 3660 5005
Size: 53 1511 545 815 174 391 27 1345
Cuts at: 5005 144
Size: 622
Fragments arranged by size:
1511 1345 815 622 545 391 174 53
27
EagI C'GGCC_G
Cuts at: 3068 3068
Size: 5483
EarI CTCTTCn'nnn_
Cuts at: 349 1273 2101 3506 3716 349
Size: 924 828 1405 210 2116
Fragments arranged by size:
2116 1405 924 828 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 391 2481 3691 4001 4147 5293 391
Size: 2090 1210 310 146 1146 581
Fragments arranged by size:
2090 1210 1146 581 310 146
EcoICRI GAG'CTC
Cuts at: 255 2495 255
Size: 2240 3243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 772 952 1153 1402 1528 1738 3308 3740
Size: 273 180 201 249 126 210 1570 432
Cuts at: 3740 4487 4755 499
Size: 747 268 1227
Fragments arranged by size:
1570 1227 747 432 273 268 249 210
201 180 126
EcoNI CCTnn'n_nnAGG
Cuts at: 1593 1593
Size: 5483
EcoO109I rG'GnC_Cy
Cuts at: 289 429 600 601 925 1327 1503 2622 289
Size: 140 171 1 324 402 176 1119 3150
Fragments arranged by size:
3150 1119 402 324 176 171 140 1
EcoRI G'AATT_C
Cuts at: 2487 2487
Size: 5483
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 360 3502 360
Size: 3142 2341
FspI TGC'GCA
Cuts at: 3264 3264
Size: 5483
HaeII r_GCGC'y
Cuts at: 1057 2917 3165 4187 5166 1057
Size: 1860 248 1022 979 1374
Fragments arranged by size:
1860 1374 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 723 755 1900 1905 1932 1937 3325 3355 3357
Size: 32 1145 5 27 5 1388 30 2
Cuts at: 3357 3387 723
Size: 30 2819
Fragments arranged by size:
2819 1388 1145 32 30 30 27 5
5 2
HincII GTy'rAC
Cuts at: 1227 2001 2522 1227
Size: 774 521 4188
Fragments arranged by size:
4188 774 521
KasI G'GCGC_C
Cuts at: 1053 2913 3161 1053
Size: 1860 248 3375
Fragments arranged by size:
3375 1860 248
KpnI G_GTAC'C
Cuts at: 2503 2503
Size: 5483
MluI A'CGCG_T
Cuts at: 4916 4916
Size: 5483
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 1928 4154 4338 263
Size: 1665 2226 184 1408
Fragments arranged by size:
2226 1665 1408 184
MscI TGG'CCA
Cuts at: 199 1710 3244 199
Size: 1511 1534 2438
Fragments arranged by size:
2438 1534 1511
MslI CAynn'nnrTG
Cuts at: 793 3472 3599 793
Size: 2679 127 2677
Fragments arranged by size:
2679 2677 127
MspA1I CmG'CkG
Cuts at: 990 1307 2594 2908 3268 4281 4526 990
Size: 317 1287 314 360 1013 245 1947
Fragments arranged by size:
1947 1287 1013 360 317 314 245
NaeI GCC'GGC
Cuts at: 3664 3664
Size: 5483
NarI GG'CG_CC
Cuts at: 1054 2914 3162 1054
Size: 1860 248 3375
Fragments arranged by size:
3375 1860 248
NgoMIV G'CCGG_C
Cuts at: 3662 3662
Size: 5483
NsiI A_TGCA'T
Cuts at: 964 964
Size: 5483
NspI r_CATG'y
Cuts at: 498 1659 1992 2536 3036 3943 498
Size: 1161 333 544 500 907 2038
Fragments arranged by size:
2038 1161 907 544 500 333
PasI CC'CwG_GG
Cuts at: 1353 1353
Size: 5483
PciI A'CATG_T
Cuts at: 3939 3939
Size: 5483
PflMI CCAn_nnn'nTGG
Cuts at: 557 1152 557
Size: 595 4888
PfoI T'CCnGG_A
Cuts at: 1082 1442 1082
Size: 360 5123
PmeI GTTT'AAAC
Cuts at: 2482 2482
Size: 5483
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 960 4622 4654 928
Size: 32 3662 32 1757
Fragments arranged by size:
3662 1757 32 32
PpuMI rG'GwC_Cy
Cuts at: 289 429 925 1327 1503 289
Size: 140 496 402 176 4269
Fragments arranged by size:
4269 496 402 176 140
PspOMI G'GGCC_C
Cuts at: 600 600
Size: 5483
PstI C_TGCA'G
Cuts at: 248 783 2057 2477 2530 3215 248
Size: 535 1274 420 53 685 2516
Fragments arranged by size:
2516 1274 685 535 420 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5483
PvuII CAG'CTG
Cuts at: 990 2908 3268 990
Size: 1918 360 3205
Fragments arranged by size:
3205 1918 360
RsrII CG'GwC_CG
Cuts at: 3678 3678
Size: 5483
SacI G_AGCT'C
Cuts at: 257 2497 257
Size: 2240 3243
SalI G'TCGA_C
Cuts at: 2520 2520
Size: 5483
SanDI GG'GwC_CC
Cuts at: 1327 1327
Size: 5483
SapI GCTCTTCn'nnn_
Cuts at: 1273 3506 3716 1273
Size: 2233 210 3040
Fragments arranged by size:
3040 2233 210
SbfI CC_TGCA'GG
Cuts at: 2530 2530
Size: 5483
ScaI AGT'ACT
Cuts at: 5000 5000
Size: 5483
SfcI C'TryA_G
Cuts at: 33 244 434 779 2053 2473 2526 3211 4204
Size: 211 190 345 1274 420 53 685 993
Cuts at: 4204 4395 33
Size: 191 1121
Fragments arranged by size:
1274 1121 993 685 420 345 211 191
190 53
SfoI GGC'GCC
Cuts at: 1055 2915 3163 1055
Size: 1860 248 3375
Fragments arranged by size:
3375 1860 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5483
SmaI CCC'GGG
Cuts at: 2460 2505 2460
Size: 45 5438
SmlI C'TyrA_G
Cuts at: 293 2317 2638 3553 4045 4307 293
Size: 2024 321 915 492 262 1469
Fragments arranged by size:
2024 1469 915 492 321 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5483
SphI G_CATG'C
Cuts at: 498 1992 2536 3036 498
Size: 1494 544 500 2945
Fragments arranged by size:
2945 1494 544 500
StyI C'CwwG_G
Cuts at: 260 1169 2617 2971 260
Size: 909 1448 354 2772
Fragments arranged by size:
2772 1448 909 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 3066 3199 3658 3867 1746
Size: 1320 133 459 209 3362
Fragments arranged by size:
3362 1320 459 209 133
TatI w'GTAC_w
Cuts at: 501 2012 4998 501
Size: 1511 2986 986
Fragments arranged by size:
2986 1511 986
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 370 402 1003 1035 1064 1096 2264 2296 5000
Size: 32 601 32 29 32 1168 32 2704
Cuts at: 5000 5032 370
Size: 32 821
Fragments arranged by size:
2704 1168 821 601 32 32 32 32
32 29
Tth111I GACn'n_nGTC
Cuts at: 1786 3280 1786
Size: 1494 3989
XbaI T'CTAG_A
Cuts at: 2514 2514
Size: 5483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1884 2978 1884
Size: 1094 4389
XmaI C'CCGG_G
Cuts at: 2458 2503 2458
Size: 45 5438
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BaeI
BcgI BmtI BsiWI BsmBI BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI