Restriction map for
pF1KA1507
(Circular) (Six-base) MAPSORT of: pF1KA1507.seq Check: 6040 from: 1 to: 5879
pF1KA1507 5879 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 890 2368 890
Size: 1478 4401
AccI GT'mk_AC
Cuts at: 1466 2534 2917 1466
Size: 1068 383 4428
Fragments arranged by size:
4428 1068 383
Acc65I G'GTAC_C
Cuts at: 2895 2895
Size: 5879
AclI AA'CG_TT
Cuts at: 5719 5719
Size: 5879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 2280 3704 4136 4489 5151 2137
Size: 143 1424 432 353 662 2865
Fragments arranged by size:
2865 1424 662 432 353 143
AflIII A'CryG_T
Cuts at: 1320 3231 5031 5312 1320
Size: 1911 1800 281 1887
Fragments arranged by size:
1911 1887 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1104 1104
Size: 5879
AleI CACnn'nnGTG
Cuts at: 481 1882 481
Size: 1401 4478
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1594 1626 1594
Size: 32 5847
AlwNI CAG_nnn'CTG
Cuts at: 1072 2287 2307 2595 3184 4622 1072
Size: 1215 20 288 589 1438 2329
Fragments arranged by size:
2329 1438 1215 589 288 20
ApaI G_GGCC'C
Cuts at: 402 402
Size: 5879
ApaLI G'TGCA_C
Cuts at: 4717 4717
Size: 5879
ApoI r'AATT_y
Cuts at: 429 1019 2883 5232 5488 429
Size: 590 1864 2349 256 820
Fragments arranged by size:
2349 1864 820 590 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5879
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5879
AvaI C'yCGr_G
Cuts at: 118 933 992 1716 1737 2255 2899 118
Size: 815 59 724 21 518 644 3098
Fragments arranged by size:
3098 815 724 644 518 59 21
AvrII C'CTAG_G
Cuts at: 1911 2569 1911
Size: 658 5221
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 605 638 605
Size: 33 5846
BamHI G'GATC_C
Cuts at: 1370 2598 2904 1370
Size: 1228 306 4345
Fragments arranged by size:
4345 1228 306
BanI G'GyrC_C
Cuts at: 858 982 1401 1873 2178 2895 3309 3557 3592
Size: 124 419 472 305 717 414 248 35
Cuts at: 3592 858
Size: 3145
Fragments arranged by size:
3145 717 472 419 414 305 248 124
35
BanII G_rGCy'C
Cuts at: 402 1910 2114 2893 3923 402
Size: 1508 204 779 1030 2358
Fragments arranged by size:
2358 1508 1030 779 204
BbeI G_GCGC'C
Cuts at: 862 3313 3561 862
Size: 2451 248 3180
Fragments arranged by size:
3180 2451 248
BbsI GAAGACnn'nnnn_
Cuts at: 842 842
Size: 5879
BbvCI CC'TCA_GC
Cuts at: 928 1561 1663 2288 928
Size: 633 102 625 4519
Fragments arranged by size:
4519 633 625 102
BciVI GTATCCnnnnn_n'
Cuts at: 3777 4833 5879 3777
Size: 1056 1046 3777
Fragments arranged by size:
3777 1056 1046
BclI T'GATC_A
Cuts at: 2438 3399 2438
Size: 961 4918
BfrBI ATG'CAT
Cuts at: 2485 2485
Size: 5879
BglI GCCn_nnn'nGGC
Cuts at: 1899 1899
Size: 5879
BglII A'GATC_T
Cuts at: 3394 5431 3394
Size: 2037 3842
BlpI GC'TnA_GC
Cuts at: 1658 2083 2991 1658
Size: 425 908 4546
Fragments arranged by size:
4546 908 425
Bme1580I G_kGCm'C
Cuts at: 402 648 985 1404 1876 2513 2664 3504 3597
Size: 246 337 419 472 637 151 840 93
Cuts at: 3597 4721 402
Size: 1124 1560
Fragments arranged by size:
1560 1124 840 637 472 419 337 246
151 93
BmgBI CAC'GTC
Cuts at: 492 1884 2130 492
Size: 1392 246 4241
Fragments arranged by size:
4241 1392 246
BmrI ACTGGGnnnn_n'
Cuts at: 405 561 1462 3091 3172 3507 5651 405
Size: 156 901 1629 81 335 2144 633
Fragments arranged by size:
2144 1629 901 633 335 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1752 1850 4246 1752
Size: 98 2396 3385
Fragments arranged by size:
3385 2396 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1602 1606 2080 2158 2410 340
Size: 1262 4 474 78 252 3809
Fragments arranged by size:
3809 1262 474 252 78 4
Bpu10I CC'TnA_GC
Cuts at: 260 928 1561 1663 1904 2288 2614 260
Size: 668 633 102 241 384 326 3525
Fragments arranged by size:
3525 668 633 384 326 241 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1779 3055 3934 4648 4946 1779
Size: 1276 879 714 298 2712
Fragments arranged by size:
2712 1276 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1112 1851 5583 1112
Size: 739 3732 1408
Fragments arranged by size:
3732 1408 739
BsaAI yAC'GTr
Cuts at: 3232 3232
Size: 5879
BsaBI GATnn'nnATC
Cuts at: 6 3393 5430 6
Size: 3387 2037 455
Fragments arranged by size:
3387 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 503 859 887 2365 3310 3558 4260 5756
Size: 391 356 28 1478 945 248 702 1496
Cuts at: 5756 112
Size: 235
Fragments arranged by size:
1496 1478 945 702 391 356 248 235
28
BsaWI w'CCGG_w
Cuts at: 1502 1836 3068 3589 4267 4678 4825 5099 1502
Size: 334 1232 521 678 411 147 274 2282
Fragments arranged by size:
2282 1232 678 521 411 334 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 338 368 1594 1624 2131 2161 338
Size: 30 1226 30 507 30 4056
Fragments arranged by size:
4056 1226 507 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 553 648 1134 1495 1704 2194 553
Size: 95 486 361 209 490 4238
Fragments arranged by size:
4238 490 486 361 209 95
BseYI C'CCAG_C
Cuts at: 558 803 908 1036 1814 2099 2371 3304 4062
Size: 245 105 128 778 285 272 933 758
Cuts at: 4062 4727 5135 558
Size: 665 408 1302
Fragments arranged by size:
1302 933 778 758 665 408 285 272
245 128 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1744 2110 1744
Size: 366 5513
BsiEI CG_ry'CG
Cuts at: 104 3421 3467 4697 104
Size: 3317 46 1230 1286
Fragments arranged by size:
3317 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 497 2080 2736 2893 3671 3861 4721 497
Size: 1583 656 157 778 190 860 1655
Fragments arranged by size:
1655 1583 860 778 656 190 157
BsmI GAATG_Cn'
Cuts at: 123 1694 123
Size: 1571 4308
BsmBI CGTCTCn'nnnn_
Cuts at: 162 322 1834 2136 2859 162
Size: 160 1512 302 723 3182
Fragments arranged by size:
3182 1512 723 302 160
Bsp1286I G_dGCh'C
Cuts at: 402 497 648 985 1404 1876 1910 2080 2114
Size: 95 151 337 419 472 34 170 34
Cuts at: 2114 2513 2664 2736 2893 3504 3597 3671 3861
Size: 399 151 72 157 611 93 74 190
Cuts at: 3861 3923 4721 402
Size: 62 798 1560
Fragments arranged by size:
1560 798 611 472 419 399 337 190
170 157 151 151 95 93 74 72
62 34 34
BspEI T'CCGG_A
Cuts at: 1836 1836
Size: 5879
BspHI T'CATG_A
Cuts at: 2547 4311 2547
Size: 1764 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2929 3143 3445 3826 2929
Size: 214 302 381 4982
Fragments arranged by size:
4982 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1896 4171 4225 5709 5874 50
Size: 1846 2275 54 1484 165 55
Fragments arranged by size:
2275 1846 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 185 2433 3791 185
Size: 2248 1358 2273
Fragments arranged by size:
2273 2248 1358
BsrFI r'CCGG_y
Cuts at: 1989 3877 4058 1989
Size: 1888 181 3810
Fragments arranged by size:
3810 1888 181
BssSI C'ACGA_G
Cuts at: 724 4150 4858 724
Size: 3426 708 1745
Fragments arranged by size:
3426 1745 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3256 3256
Size: 5879
BstBI TT'CG_AA
Cuts at: 1686 4240 1686
Size: 2554 3325
BstEII G'GTnAC_C
Cuts at: 2608 2608
Size: 5879
BstXI CCAn_nnnn'nTGG
Cuts at: 513 1884 2723 513
Size: 1371 839 3669
Fragments arranged by size:
3669 1371 839
BstYI r'GATC_y
Cuts at: 1 1370 2598 2904 3371 3394 3729 3975 4281
Size: 1369 1228 306 467 23 335 246 306
Cuts at: 4281 4293 4379 5431 1
Size: 12 86 1052 449
Fragments arranged by size:
1369 1228 1052 467 449 335 306 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2535 2535
Size: 5879
Bsu36I CC'TnA_GG
Cuts at: 404 436 1780 404
Size: 32 1344 4503
Fragments arranged by size:
4503 1344 32
BtgI C'CryG_G
Cuts at: 107 1251 1631 2741 107
Size: 1144 380 1110 3245
Fragments arranged by size:
3245 1144 1110 380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2663 3829 4010 4173 2663
Size: 1166 181 163 4369
Fragments arranged by size:
4369 1166 181 163
BtsI GCAGTG_nn'
Cuts at: 952 2756 3122 952
Size: 1804 366 3709
Fragments arranged by size:
3709 1804 366
ClaI AT'CG_AT
Cuts at: 2069 2069
Size: 5879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 710 745 2138 2173 710
Size: 35 1393 35 4416
Fragments arranged by size:
4416 1393 35 35
DraI TTT'AAA
Cuts at: 2878 2878
Size: 5879
DraIII CAC_nnn'GTG
Cuts at: 2608 2608
Size: 5879
DrdI GACnn_nn'nnGTC
Cuts at: 798 844 3585 4929 798
Size: 46 2741 1344 1748
Fragments arranged by size:
2741 1748 1344 46
EaeI y'GGCC_r
Cuts at: 182 1701 2402 2744 3464 3638 4029 4056 5401
Size: 1519 701 342 720 174 391 27 1345
Cuts at: 5401 182
Size: 660
Fragments arranged by size:
1519 1345 720 701 660 391 342 174
27
EagI C'GGCC_G
Cuts at: 3464 3464
Size: 5879
EarI CTCTTCn'nnn_
Cuts at: 530 1360 2236 2782 3902 4112 530
Size: 830 876 546 1120 210 2297
Fragments arranged by size:
2297 1120 876 830 546 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4087 4829 4975 5689 4087
Size: 742 146 714 4277
Fragments arranged by size:
4277 742 714 146
EcoICRI GAG'CTC
Cuts at: 2891 2891
Size: 5879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1602 1606 2080 2137 2158 2280 2410 3704
Size: 1262 4 474 57 21 122 130 1294
Cuts at: 3704 4136 4489 5151 340
Size: 432 353 662 1068
Fragments arranged by size:
1294 1262 1068 662 474 432 353 130
122 57 21 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1330 1548 1330
Size: 218 5661
EcoO109I rG'GnC_Cy
Cuts at: 399 450 555 969 1575 1797 2261 2304 2357
Size: 51 105 414 606 222 464 43 53
Cuts at: 2357 3018 399
Size: 661 3260
Fragments arranged by size:
3260 661 606 464 414 222 105 53
51 43
EcoRI G'AATT_C
Cuts at: 2883 2883
Size: 5879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1791 3898 1791
Size: 2107 3772
FspI TGC'GCA
Cuts at: 880 3660 880
Size: 2780 3099
FspAI rTGC'GCAy
Cuts at: 880 880
Size: 5879
HaeII r_GCGC'y
Cuts at: 862 3313 3561 4791 5562 862
Size: 2451 248 1230 771 1179
Fragments arranged by size:
2451 1230 1179 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1085 1090 1117 1122 1699 1731 2131 2163 2235
Size: 5 27 5 577 32 400 32 72
Cuts at: 2235 2267 2783 2815 3721 3751 3753 3783 1085
Size: 32 516 32 906 30 2 30 3181
Fragments arranged by size:
3181 906 577 516 400 72 32 32
32 32 30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 1217 1467 2168 2918 1217
Size: 250 701 750 4178
Fragments arranged by size:
4178 750 701 250
KasI G'GCGC_C
Cuts at: 858 3309 3557 858
Size: 2451 248 3180
Fragments arranged by size:
3180 2451 248
KpnI G_GTAC'C
Cuts at: 2899 2899
Size: 5879
MluI A'CGCG_T
Cuts at: 5312 5312
Size: 5879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 301 2157 4638 4822 301
Size: 1856 2481 184 1358
Fragments arranged by size:
2481 1856 1358 184
MscI TGG'CCA
Cuts at: 184 2404 3640 184
Size: 2220 1236 2423
Fragments arranged by size:
2423 2220 1236
MslI CAynn'nnrTG
Cuts at: 481 1319 1882 2281 2326 3868 3995 481
Size: 838 563 399 45 1542 127 2365
Fragments arranged by size:
2365 1542 838 563 399 127 45
MspA1I CmG'CkG
Cuts at: 178 562 1040 1150 2099 2371 2990 3304 3664
Size: 384 478 110 949 272 619 314 360
Cuts at: 3664 4448 4693 178
Size: 784 245 1364
Fragments arranged by size:
1364 949 784 619 478 384 360 314
272 245 110
NaeI GCC'GGC
Cuts at: 1991 4060 1991
Size: 2069 3810
NarI GG'CG_CC
Cuts at: 859 3310 3558 859
Size: 2451 248 3180
Fragments arranged by size:
3180 2451 248
NcoI C'CATG_G
Cuts at: 107 2741 107
Size: 2634 3245
NgoMIV G'CCGG_C
Cuts at: 1989 4058 1989
Size: 2069 3810
NsiI A_TGCA'T
Cuts at: 2487 2487
Size: 5879
NspI r_CATG'y
Cuts at: 1324 2091 2485 2517 2932 3432 5035 1324
Size: 767 394 32 415 500 1603 2168
Fragments arranged by size:
2168 1603 767 500 415 394 32
PciI A'CATG_T
Cuts at: 1320 5031 1320
Size: 3711 2168
PflMI CCAn_nnn'nTGG
Cuts at: 2307 2325 2307
Size: 18 5861
PfoI T'CCnGG_A
Cuts at: 356 830 2594 356
Size: 474 1764 3641
Fragments arranged by size:
3641 1764 474
PmeI GTTT'AAAC
Cuts at: 2878 2878
Size: 5879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1594 1626 4325 4357 1594
Size: 32 2699 32 3116
Fragments arranged by size:
3116 2699 32 32
PpuMI rG'GwC_Cy
Cuts at: 555 969 1575 2304 555
Size: 414 606 729 4130
Fragments arranged by size:
4130 729 606 414
PspOMI G'GGCC_C
Cuts at: 398 398
Size: 5879
PspXI vC'TCGA_Gb
Cuts at: 2255 2255
Size: 5879
PstI C_TGCA'G
Cuts at: 371 1121 1411 1571 2628 2926 3611 371
Size: 750 290 160 1057 298 685 2639
Fragments arranged by size:
2639 1057 750 685 298 290 160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5879
PvuII CAG'CTG
Cuts at: 178 562 2371 3304 3664 178
Size: 384 1809 933 360 2393
Fragments arranged by size:
2393 1809 933 384 360
RsrII CG'GwC_CG
Cuts at: 4074 4074
Size: 5879
SacI G_AGCT'C
Cuts at: 2893 2893
Size: 5879
SalI G'TCGA_C
Cuts at: 1465 2916 1465
Size: 1451 4428
SanDI GG'GwC_CC
Cuts at: 555 1575 555
Size: 1020 4859
SapI GCTCTTCn'nnn_
Cuts at: 2236 3902 4112 2236
Size: 1666 210 4003
Fragments arranged by size:
4003 1666 210
SbfI CC_TGCA'GG
Cuts at: 1411 2926 1411
Size: 1515 4364
ScaI AGT'ACT
Cuts at: 1774 5396 1774
Size: 3622 2257
SexAI A'CCwGG_T
Cuts at: 335 2604 335
Size: 2269 3610
SfcI C'TryA_G
Cuts at: 33 367 1117 1407 1545 1567 2624 2763 2922
Size: 334 750 290 138 22 1057 139 159
Cuts at: 2922 3607 4575 4766 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1057 968 750 685 334 290 191
159 139 138 22
SfoI GGC'GCC
Cuts at: 860 3311 3559 860
Size: 2451 248 3180
Fragments arranged by size:
3180 2451 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5879
SmaI CCC'GGG
Cuts at: 120 935 994 2901 120
Size: 815 59 1907 3098
Fragments arranged by size:
3098 1907 815 59
SmlI C'TyrA_G
Cuts at: 1758 2255 3034 3949 4663 4925 1758
Size: 497 779 915 714 262 2712
Fragments arranged by size:
2712 915 779 714 497 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5879
SphI G_CATG'C
Cuts at: 2091 2485 2932 3432 2091
Size: 394 447 500 4538
Fragments arranged by size:
4538 500 447 394
SrfI GCCC'GGGC
Cuts at: 120 935 120
Size: 815 5064
StuI AGG'CCT
Cuts at: 510 2672 2720 510
Size: 2162 48 3669
Fragments arranged by size:
3669 2162 48
StyI C'CwwG_G
Cuts at: 107 811 1911 2399 2456 2569 2741 3013 3367
Size: 704 1100 488 57 113 172 272 354
Cuts at: 3367 107
Size: 2619
Fragments arranged by size:
2619 1100 704 488 354 272 172 113
57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3462 3595 4054 4263 3462
Size: 133 459 209 5078
Fragments arranged by size:
5078 459 209 133
TatI w'GTAC_w
Cuts at: 1199 1772 5394 1199
Size: 573 3622 1684
Fragments arranged by size:
3622 1684 573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5396 5428 5396
Size: 32 5847
Tth111I GACn'n_nGTC
Cuts at: 3676 3676
Size: 5879
XbaI T'CTAG_A
Cuts at: 2910 2910
Size: 5879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 483 2412 3374 192
Size: 291 1929 962 2697
Fragments arranged by size:
2697 1929 962 291
XhoI C'TCGA_G
Cuts at: 2255 2255
Size: 5879
XmaI C'CCGG_G
Cuts at: 118 933 992 2899 118
Size: 815 59 1907 3098
Fragments arranged by size:
3098 1907 815 59
XmnI GAAnn'nnTTC
Cuts at: 1609 1609
Size: 5879
ZraI GAC'GTC
Cuts at: 888 2366 888
Size: 1478 4401
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AlfI AscI BcgI BmtI
BsiWI BsrGI BssHII EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PsrI SacII SfiI SgrAI SnaBI SspI SwaI