Restriction map for
pF1KA1508
(Circular) (Six-base) MAPSORT of: pF1KA1508.seq Check: 5159 from: 1 to: 6491
pF1KA1508 6491 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 762 2287 762
Size: 1525 4966
AatII G_ACGT'C
Cuts at: 3256 3256
Size: 6491
AccI GT'mk_AC
Cuts at: 3529 3529
Size: 6491
Acc65I G'GTAC_C
Cuts at: 320 939 3507 320
Size: 619 2568 3304
Fragments arranged by size:
3304 2568 619
AclI AA'CG_TT
Cuts at: 6331 6331
Size: 6491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 2072 2094 3327 4316 4748 5101 5763 295
Size: 1777 22 1233 989 432 353 662 1023
Fragments arranged by size:
1777 1233 1023 989 662 432 353 22
AfeI AGC'GCT
Cuts at: 1912 2962 1912
Size: 1050 5441
AflIII A'CryG_T
Cuts at: 2375 3843 5643 5924 2375
Size: 1468 1800 281 2942
Fragments arranged by size:
2942 1800 1468 281
AgeI A'CCGG_T
Cuts at: 1007 1007
Size: 6491
AhdI GACnn_n'nnGTC
Cuts at: 3462 3462
Size: 6491
AleI CACnn'nnGTG
Cuts at: 673 673
Size: 6491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 898 1763 2203 3460 898
Size: 865 440 1257 3929
Fragments arranged by size:
3929 1257 865 440
AlwNI CAG_nnn'CTG
Cuts at: 871 1284 1786 2664 3796 5234 871
Size: 413 502 878 1132 1438 2128
Fragments arranged by size:
2128 1438 1132 878 502 413
ApaI G_GGCC'C
Cuts at: 130 990 1253 1485 2567 130
Size: 860 263 232 1082 4054
Fragments arranged by size:
4054 1082 860 263 232
ApaLI G'TGCA_C
Cuts at: 262 5329 262
Size: 5067 1424
ApoI r'AATT_y
Cuts at: 3151 3495 5844 6100 3151
Size: 344 2349 256 3542
Fragments arranged by size:
3542 2349 344 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6491
AvaI C'yCGr_G
Cuts at: 250 650 735 983 1235 1294 1478 2260 3019
Size: 400 85 248 252 59 184 782 759
Cuts at: 3019 3459 3511 250
Size: 440 52 3230
Fragments arranged by size:
3230 782 759 440 400 252 248 184
85 59 52
AvrII C'CTAG_G
Cuts at: 148 148
Size: 6491
BamHI G'GATC_C
Cuts at: 3516 3516
Size: 6491
BanI G'GyrC_C
Cuts at: 152 320 675 817 882 939 965 1091 1795
Size: 168 355 142 65 57 26 126 704
Cuts at: 1795 1970 2044 2313 3507 3921 4169 4204 152
Size: 175 74 269 1194 414 248 35 2439
Fragments arranged by size:
2439 1194 704 414 355 269 248 175
168 142 126 74 65 57 35 26
BanII G_rGCy'C
Cuts at: 130 335 686 743 845 990 1101 1253 1485
Size: 205 351 57 102 145 111 152 232
Cuts at: 1485 1709 2261 2567 3505 4535 130
Size: 224 552 306 938 1030 2086
Fragments arranged by size:
2086 1030 938 552 351 306 232 224
205 152 145 111 102 57
BbeI G_GCGC'C
Cuts at: 156 1974 2317 3925 4173 156
Size: 1818 343 1608 248 2474
Fragments arranged by size:
2474 1818 1608 343 248
BbsI GAAGACnn'nnnn_
Cuts at: 374 451 1679 374
Size: 77 1228 5186
Fragments arranged by size:
5186 1228 77
BbvCI CC'TCA_GC
Cuts at: 690 2423 690
Size: 1733 4758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 165 199 1081 1115 165
Size: 34 882 34 5541
Fragments arranged by size:
5541 882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4389 5445 6491 4389
Size: 1056 1046 4389
Fragments arranged by size:
4389 1056 1046
BclI T'GATC_A
Cuts at: 2516 2981 4011 2516
Size: 465 1030 4996
Fragments arranged by size:
4996 1030 465
BglI GCCn_nnn'nGGC
Cuts at: 385 985 1076 1085 1280 1343 1660 2606 385
Size: 600 91 9 195 63 317 946 4270
Fragments arranged by size:
4270 946 600 317 195 91 63 9
BglII A'GATC_T
Cuts at: 4006 6043 4006
Size: 2037 4454
BlpI GC'TnA_GC
Cuts at: 2239 3603 2239
Size: 1364 5127
Bme1580I G_kGCm'C
Cuts at: 130 266 768 887 970 990 1161 1253 1485
Size: 136 502 119 83 20 171 92 232
Cuts at: 1485 1798 2047 2567 2885 3407 3449 4116 4209
Size: 313 249 520 318 522 42 667 93
Cuts at: 4209 5333 130
Size: 1124 1288
Fragments arranged by size:
1288 1124 667 522 520 502 318 313
249 232 171 136 119 93 92 83
42 20
BmgBI CAC'GTC
Cuts at: 1743 1743
Size: 6491
BmrI ACTGGGnnnn_n'
Cuts at: 133 695 1156 1404 3703 3784 4119 6263 133
Size: 562 461 248 2299 81 335 2144 361
Fragments arranged by size:
2299 2144 562 461 361 335 248 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 705 4858 705
Size: 4153 2338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 514 602 747 919 1507 1749 2794 2878
Size: 165 88 145 172 588 242 1045 84
Cuts at: 2878 349
Size: 3962
Fragments arranged by size:
3962 1045 588 242 172 165 145 88
84
Bpu10I CC'TnA_GC
Cuts at: 680 690 2423 680
Size: 10 1733 4748
Fragments arranged by size:
4748 1733 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1137 2596 2611 2980 3154 3667 4546 5260 5558
Size: 1459 15 369 174 513 879 714 298
Cuts at: 5558 1137
Size: 2070
Fragments arranged by size:
2070 1459 879 714 513 369 298 174
15
BsaI GGTCTCn'nnnn_
Cuts at: 398 1767 2353 6195 398
Size: 1369 586 3842 694
Fragments arranged by size:
3842 1369 694 586
BsaAI yAC'GTr
Cuts at: 2376 2644 3844 2376
Size: 268 1200 5023
Fragments arranged by size:
5023 1200 268
BsaBI GATnn'nnATC
Cuts at: 6 4005 6042 6
Size: 3999 2037 455
Fragments arranged by size:
3999 2037 455
BsaHI Gr'CG_yC
Cuts at: 153 850 1836 1971 2314 3253 3922 4170 4872
Size: 697 986 135 343 939 669 248 702
Cuts at: 4872 6368 153
Size: 1496 276
Fragments arranged by size:
1496 986 939 702 697 669 343 276
248 135
BsaWI w'CCGG_w
Cuts at: 1007 1310 2006 3680 4201 4879 5290 5437 5711
Size: 303 696 1674 521 678 411 147 274
Cuts at: 5711 1007
Size: 1787
Fragments arranged by size:
1787 1674 696 678 521 411 303 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 949 979 949
Size: 30 6461
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 317 825 1030 1339 1872 2382 2791 317
Size: 508 205 309 533 510 409 4017
Fragments arranged by size:
4017 533 510 508 409 309 205
BseYI C'CCAG_C
Cuts at: 808 889 969 1074 1190 1199 1471 1717 1990
Size: 81 80 105 116 9 272 246 273
Cuts at: 1990 2039 2442 3916 4674 5339 5747 808
Size: 49 403 1474 758 665 408 1552
Fragments arranged by size:
1552 1474 758 665 408 403 273 272
246 116 105 81 80 49 9
BsiEI CG_ry'CG
Cuts at: 104 260 1465 2291 4033 4079 5309 104
Size: 156 1205 826 1742 46 1230 1286
Fragments arranged by size:
1742 1286 1230 1205 826 156 46
BsiHKAI G_wGCw'C
Cuts at: 145 266 1101 3505 4283 4473 5333 145
Size: 121 835 2404 778 190 860 1303
Fragments arranged by size:
2404 1303 860 835 778 190 121
BsmBI CGTCTCn'nnnn_
Cuts at: 954 954
Size: 6491
Bsp1286I G_dGCh'C
Cuts at: 130 145 266 335 686 743 768 845 887
Size: 15 121 69 351 57 25 77 42
Cuts at: 887 970 990 1101 1161 1253 1485 1709 1798
Size: 83 20 111 60 92 232 224 89
Cuts at: 1798 2047 2261 2567 2885 3407 3449 3505 4116
Size: 249 214 306 318 522 42 56 611
Cuts at: 4116 4209 4283 4473 4535 5333 130
Size: 93 74 190 62 798 1288
Fragments arranged by size:
1288 798 611 522 351 318 306 249
232 224 214 190 121 111 93 92
89 83 77 74 69 62 60 57
56 42 42 25 20 15
BspEI T'CCGG_A
Cuts at: 2006 2006
Size: 6491
BspHI T'CATG_A
Cuts at: 4923 4923
Size: 6491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 762 1250 1898 2287 2672 2957 3541 3755 4057
Size: 488 648 389 385 285 584 214 302
Cuts at: 4057 4438 762
Size: 381 2815
Fragments arranged by size:
2815 648 584 488 389 385 381 302
285 214
BsrBI CCG'CTC
Cuts at: 50 1182 1880 2250 2749 4783 4837 6321 6486
Size: 1132 698 370 499 2034 54 1484 165
Cuts at: 6486 50
Size: 55
Fragments arranged by size:
2034 1484 1132 698 499 370 165 55
54
BsrDI GCAATG_nn'
Cuts at: 4403 4403
Size: 6491
BsrFI r'CCGG_y
Cuts at: 407 1007 1342 2265 2290 4489 4670 407
Size: 600 335 923 25 2199 181 2228
Fragments arranged by size:
2228 2199 923 600 335 181 25
BsrGI T'GTAC_A
Cuts at: 191 191
Size: 6491
BssHII G'CGCG_C
Cuts at: 2224 2224
Size: 6491
BssSI C'ACGA_G
Cuts at: 2338 2849 4762 5470 2338
Size: 511 1913 708 3359
Fragments arranged by size:
3359 1913 708 511
BstAPI GCAn_nnn'nTGC
Cuts at: 3868 3868
Size: 6491
BstBI TT'CG_AA
Cuts at: 4852 4852
Size: 6491
BstEII G'GTnAC_C
Cuts at: 3279 3279
Size: 6491
BstXI CCAn_nnnn'nTGG
Cuts at: 897 2560 897
Size: 1663 4828
BstYI r'GATC_y
Cuts at: 1 3120 3516 3983 4006 4341 4587 4893 4905
Size: 3119 396 467 23 335 246 306 12
Cuts at: 4905 4991 6043 1
Size: 86 1052 449
Fragments arranged by size:
3119 1052 467 449 396 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1525 2650 3395 1525
Size: 1125 745 4621
Fragments arranged by size:
4621 1125 745
BtgI C'CryG_G
Cuts at: 107 297 335 974 1036 1904 2792 2928 107
Size: 190 38 639 62 868 888 136 3670
Fragments arranged by size:
3670 888 868 639 190 136 62 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1526 1940 3162 4441 4622 4785 1526
Size: 414 1222 1279 181 163 3232
Fragments arranged by size:
3232 1279 1222 414 181 163
BtsI GCAGTG_nn'
Cuts at: 264 694 2903 3407 3734 264
Size: 430 2209 504 327 3021
Fragments arranged by size:
3021 2209 504 430 327
DraI TTT'AAA
Cuts at: 3490 3490
Size: 6491
DrdI GACnn_nn'nnGTC
Cuts at: 4197 5541 4197
Size: 1344 5147
EaeI y'GGCC_r
Cuts at: 257 1462 1661 1872 1895 2288 4076 4250 4641
Size: 1205 199 211 23 393 1788 174 391
Cuts at: 4641 4668 6013 257
Size: 27 1345 735
Fragments arranged by size:
1788 1345 1205 735 393 391 211 199
174 27 23
EagI C'GGCC_G
Cuts at: 257 1462 2288 4076 257
Size: 1205 826 1788 2672
Fragments arranged by size:
2672 1788 1205 826
EarI CTCTTCn'nnn_
Cuts at: 278 786 3347 4514 4724 278
Size: 508 2561 1167 210 2045
Fragments arranged by size:
2561 2045 1167 508 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4699 5441 5587 6301 4699
Size: 742 146 714 4889
Fragments arranged by size:
4889 742 714 146
EcoICRI GAG'CTC
Cuts at: 1099 3503 1099
Size: 2404 4087
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 349 514 602 747 919 1507 1749 2072
Size: 54 165 88 145 172 588 242 323
Cuts at: 2072 2094 2794 2878 3327 4316 4748 5101 5763
Size: 22 700 84 449 989 432 353 662
Cuts at: 5763 295
Size: 1023
Fragments arranged by size:
1023 989 700 662 588 449 432 353
323 242 172 165 145 88 84 54
22
EcoNI CCTnn'n_nnAGG
Cuts at: 277 660 277
Size: 383 6108
EcoO109I rG'GnC_Cy
Cuts at: 307 1239 1249 1250 1257 1281 1482 2027 2490
Size: 932 10 1 7 24 201 545 463
Cuts at: 2490 2607 2661 3630 307
Size: 117 54 969 3168
Fragments arranged by size:
3168 969 932 545 463 201 117 54
24 10 7 1
EcoRI G'AATT_C
Cuts at: 3495 3495
Size: 6491
EcoRV GAT'ATC
Cuts at: 2118 2118
Size: 6491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3021 4510 3021
Size: 1489 5002
FspI TGC'GCA
Cuts at: 4272 4272
Size: 6491
HaeII r_GCGC'y
Cuts at: 156 998 1338 1914 1974 2317 2964 3925 4173
Size: 842 340 576 60 343 647 961 248
Cuts at: 4173 5403 6174 156
Size: 1230 771 473
Fragments arranged by size:
1230 961 842 771 647 576 473 343
340 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 438 470 3105 3137 3448 3480 4333
Size: 32 279 32 2635 32 311 32 853
Cuts at: 4333 4363 4365 4395 127
Size: 30 2 30 2223
Fragments arranged by size:
2635 2223 853 311 279 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3530 3530
Size: 6491
HindIII A'AGCT_T
Cuts at: 2527 2527
Size: 6491
KasI G'GCGC_C
Cuts at: 152 1970 2313 3921 4169 152
Size: 1818 343 1608 248 2474
Fragments arranged by size:
2474 1818 1608 343 248
KpnI G_GTAC'C
Cuts at: 324 943 3511 324
Size: 619 2568 3304
Fragments arranged by size:
3304 2568 619
MluI A'CGCG_T
Cuts at: 5924 5924
Size: 6491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 992 3458 5250 5434 992
Size: 2466 1792 184 2049
Fragments arranged by size:
2466 2049 1792 184
MscI TGG'CCA
Cuts at: 1663 1897 4252 1663
Size: 234 2355 3902
Fragments arranged by size:
3902 2355 234
MslI CAynn'nnrTG
Cuts at: 673 3137 4480 4607 673
Size: 2464 1343 127 2557
Fragments arranged by size:
2557 2464 1343 127
MspA1I CmG'CkG
Cuts at: 117 534 1074 1560 2183 2395 2930 3469 3602
Size: 417 540 486 623 212 535 539 133
Cuts at: 3602 3916 4276 5060 5305 117
Size: 314 360 784 245 1303
Fragments arranged by size:
1303 784 623 540 539 535 486 417
360 314 245 212 133
NaeI GCC'GGC
Cuts at: 2267 2292 4672 2267
Size: 25 2380 4086
Fragments arranged by size:
4086 2380 25
NarI GG'CG_CC
Cuts at: 153 1971 2314 3922 4170 153
Size: 1818 343 1608 248 2474
Fragments arranged by size:
2474 1818 1608 343 248
NcoI C'CATG_G
Cuts at: 107 335 974 107
Size: 228 639 5624
Fragments arranged by size:
5624 639 228
NgoMIV G'CCGG_C
Cuts at: 2265 2290 4670 2265
Size: 25 2380 4086
Fragments arranged by size:
4086 2380 25
NspI r_CATG'y
Cuts at: 2442 2831 3291 3544 4044 5647 2442
Size: 389 460 253 500 1603 3286
Fragments arranged by size:
3286 1603 500 460 389 253
PasI CC'CwG_GG
Cuts at: 1451 2711 1451
Size: 1260 5231
PciI A'CATG_T
Cuts at: 5643 5643
Size: 6491
PflMI CCAn_nnn'nTGG
Cuts at: 136 871 136
Size: 735 5756
PfoI T'CCnGG_A
Cuts at: 1367 2462 3116 1367
Size: 1095 654 4742
Fragments arranged by size:
4742 1095 654
PmeI GTTT'AAAC
Cuts at: 3490 3490
Size: 6491
PmlI CAC'GTG
Cuts at: 2376 2644 2376
Size: 268 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4937 4969 4937
Size: 32 6459
PpuMI rG'GwC_Cy
Cuts at: 307 1239 2027 2490 2661 307
Size: 932 788 463 171 4137
Fragments arranged by size:
4137 932 788 463 171
PsiI TTA'TAA
Cuts at: 2144 2144
Size: 6491
PspOMI G'GGCC_C
Cuts at: 126 986 1249 1481 2563 126
Size: 860 263 232 1082 4054
Fragments arranged by size:
4054 1082 860 263 232
PstI C_TGCA'G
Cuts at: 271 1280 1858 2697 2954 3538 4223 271
Size: 1009 578 839 257 584 685 2539
Fragments arranged by size:
2539 1009 839 685 584 578 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6491
PvuII CAG'CTG
Cuts at: 117 534 1074 1560 3916 4276 117
Size: 417 540 486 2356 360 2332
Fragments arranged by size:
2356 2332 540 486 417 360
RsrII CG'GwC_CG
Cuts at: 1340 4686 1340
Size: 3346 3145
SacI G_AGCT'C
Cuts at: 1101 3505 1101
Size: 2404 4087
SacII CC_GC'GG
Cuts at: 2931 2931
Size: 6491
SalI G'TCGA_C
Cuts at: 3528 3528
Size: 6491
SapI GCTCTTCn'nnn_
Cuts at: 4514 4724 4514
Size: 210 6281
SbfI CC_TGCA'GG
Cuts at: 1280 2954 3538 1280
Size: 1674 584 4233
Fragments arranged by size:
4233 1674 584
ScaI AGT'ACT
Cuts at: 6008 6008
Size: 6491
SexAI A'CCwGG_T
Cuts at: 935 935
Size: 6491
SfcI C'TryA_G
Cuts at: 33 267 1276 1317 1854 2352 2693 2911 2950
Size: 234 1009 41 537 498 341 218 39
Cuts at: 2950 3534 4219 5187 5378 33
Size: 584 685 968 191 1146
Fragments arranged by size:
1146 1009 968 685 584 537 498 341
234 218 191 41 39
SfiI GGCCn_nnn'nGGCC
Cuts at: 985 1280 1660 2606 985
Size: 295 380 946 4870
Fragments arranged by size:
4870 946 380 295
SfoI GGC'GCC
Cuts at: 154 1972 2315 3923 4171 154
Size: 1818 343 1608 248 2474
Fragments arranged by size:
2474 1818 1608 343 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6491
SmaI CCC'GGG
Cuts at: 652 737 985 1480 2262 3461 3513 652
Size: 85 248 495 782 1199 52 3630
Fragments arranged by size:
3630 1199 782 495 248 85 52
SmlI C'TyrA_G
Cuts at: 1116 2611 2626 2995 3169 3646 4561 5275 5537
Size: 1495 15 369 174 477 915 714 262
Cuts at: 5537 1116
Size: 2070
Fragments arranged by size:
2070 1495 915 714 477 369 262 174
15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6491
SphI G_CATG'C
Cuts at: 2831 3544 4044 2831
Size: 713 500 5278
Fragments arranged by size:
5278 713 500
SrfI GCCC'GGGC
Cuts at: 652 2262 652
Size: 1610 4881
StuI AGG'CCT
Cuts at: 913 1438 913
Size: 525 5966
StyI C'CwwG_G
Cuts at: 107 148 335 512 785 974 1584 2030 3625
Size: 41 187 177 273 189 610 446 1595
Cuts at: 3625 3979 107
Size: 354 2619
Fragments arranged by size:
2619 1595 610 446 354 273 189 187
177 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 128 1464 4074 4207 4666 4875 128
Size: 1336 2610 133 459 209 1744
Fragments arranged by size:
2610 1744 1336 459 209 133
TatI w'GTAC_w
Cuts at: 191 6006 191
Size: 5815 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 840 872 949 981 6008 6040 840
Size: 32 77 32 5027 32 1291
Fragments arranged by size:
5027 1291 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4288 4288
Size: 6491
XbaI T'CTAG_A
Cuts at: 3522 3522
Size: 6491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 669 3986 211
Size: 458 3317 2716
Fragments arranged by size:
3317 2716 458
XmaI C'CCGG_G
Cuts at: 650 735 983 1478 2260 3459 3511 650
Size: 85 248 495 782 1199 52 3630
Fragments arranged by size:
3630 1199 782 495 248 85 52
ZraI GAC'GTC
Cuts at: 3254 3254
Size: 6491
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AloI AscI BaeI BfrBI BmtI BsgI BsiWI
BsmI BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PspXI PsrI SanDI SgrAI SnaBI SspI SwaI XhoI
XmnI