Restriction map for
pF1KA1512
(Circular) (Six-base) MAPSORT of: pF1KA1512.seq Check: 8419 from: 1 to: 9062
pF1KA1512 9062 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1488 3861 5676 1488
Size: 2373 1815 4874
Fragments arranged by size:
4874 2373 1815
AccI GT'mk_AC
Cuts at: 1097 6100 1097
Size: 5003 4059
Acc65I G'GTAC_C
Cuts at: 2417 6078 2417
Size: 3661 5401
AclI AA'CG_TT
Cuts at: 8902 8902
Size: 9062
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1248 3451 5581 6887 7319 8066 8334 1248
Size: 2203 2130 1306 432 747 268 1976
Fragments arranged by size:
2203 2130 1976 1306 747 432 268
AfeI AGC'GCT
Cuts at: 553 2965 553
Size: 2412 6650
AflII C'TTAA_G
Cuts at: 5764 5764
Size: 9062
AflIII A'CryG_T
Cuts at: 1718 6414 7518 8495 1718
Size: 4696 1104 977 2285
Fragments arranged by size:
4696 2285 1104 977
AgeI A'CCGG_T
Cuts at: 7450 7450
Size: 9062
AhdI GACnn_n'nnGTC
Cuts at: 744 744
Size: 9062
AleI CACnn'nnGTG
Cuts at: 152 221 2539 4271 152
Size: 69 2318 1732 4943
Fragments arranged by size:
4943 2318 1732 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1843 1875 2671 2703 1843
Size: 32 796 32 8202
Fragments arranged by size:
8202 796 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1581 2595 2664 2871 3273 3480 3903 4260 4332
Size: 1014 69 207 402 207 423 357 72
Cuts at: 4332 4570 4573 4830 5241 5274 5697 5853 6367
Size: 238 3 257 411 33 423 156 514
Cuts at: 6367 7934 1581
Size: 1567 2709
Fragments arranged by size:
2709 1567 1014 514 423 423 411 402
357 257 238 207 207 156 72 69
33 3
ApaI G_GGCC'C
Cuts at: 3561 3645 4905 5076 6043 3561
Size: 84 1260 171 967 6580
Fragments arranged by size:
6580 1260 967 171 84
ApaLI G'TGCA_C
Cuts at: 304 3093 5582 7832 304
Size: 2789 2489 2250 1534
Fragments arranged by size:
2789 2489 2250 1534
ApoI r'AATT_y
Cuts at: 6066 8415 8671 6066
Size: 2349 256 6457
Fragments arranged by size:
6457 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9062
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9062
AvaI C'yCGr_G
Cuts at: 1448 3593 3638 6026 6082 1448
Size: 2145 45 2388 56 4428
Fragments arranged by size:
4428 2388 2145 56 45
AvrII C'CTAG_G
Cuts at: 1257 1257
Size: 9062
BamHI G'GATC_C
Cuts at: 2040 2357 4934 6087 2040
Size: 317 2577 1153 5015
Fragments arranged by size:
5015 2577 1153 317
BanI G'GyrC_C
Cuts at: 154 342 883 1303 2001 2417 4202 4687 6078
Size: 188 541 420 698 416 1785 485 1391
Cuts at: 6078 6492 6740 6775 154
Size: 414 248 35 2441
Fragments arranged by size:
2441 1785 1391 698 541 485 420 416
414 248 188 35
BanII G_rGCy'C
Cuts at: 1229 1521 2125 2141 2497 2582 3104 3561 3645
Size: 292 604 16 356 85 522 457 84
Cuts at: 3645 4697 4905 4949 5076 5255 5633 5804 6043
Size: 1052 208 44 127 179 378 171 239
Cuts at: 6043 6076 7106 1229
Size: 33 1030 3185
Fragments arranged by size:
3185 1052 1030 604 522 457 378 356
292 239 208 179 171 127 85 84
44 33 16
BbeI G_GCGC'C
Cuts at: 1307 4206 6496 6744 1307
Size: 2899 2290 248 3625
Fragments arranged by size:
3625 2899 2290 248
BbsI GAAGACnn'nnnn_
Cuts at: 1761 3545 5611 1761
Size: 1784 2066 5212
Fragments arranged by size:
5212 2066 1784
BbvCI CC'TCA_GC
Cuts at: 2789 4307 4563 5507 2789
Size: 1518 256 944 6344
Fragments arranged by size:
6344 1518 944 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3931 3965 4369 4403 3931
Size: 34 404 34 8590
Fragments arranged by size:
8590 404 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2434 6960 7721 9062 2434
Size: 4526 761 1341 2434
Fragments arranged by size:
4526 2434 1341 761
BclI T'GATC_A
Cuts at: 755 2978 4145 6582 755
Size: 2223 1167 2437 3235
Fragments arranged by size:
3235 2437 2223 1167
BfrBI ATG'CAT
Cuts at: 1856 1856
Size: 9062
BglI GCCn_nnn'nGGC
Cuts at: 925 2812 3820 4255 4357 5167 5524 5548 925
Size: 1887 1008 435 102 810 357 24 4439
Fragments arranged by size:
4439 1887 1008 810 435 357 102 24
BglII A'GATC_T
Cuts at: 1640 6577 8614 1640
Size: 4937 2037 2088
Fragments arranged by size:
4937 2088 2037
BlpI GC'TnA_GC
Cuts at: 2933 3514 3523 3691 4021 4156 4319 5249 5464
Size: 581 9 168 330 135 163 930 215
Cuts at: 5464 5726 6174 2933
Size: 262 448 5821
Fragments arranged by size:
5821 930 581 448 330 262 215 168
163 135 9
Bme1580I G_kGCm'C
Cuts at: 308 886 3097 3561 3645 4239 4611 4690 4905
Size: 578 2211 464 84 594 372 79 215
Cuts at: 4905 5076 5586 6021 6043 6687 6780 7836 308
Size: 171 510 435 22 644 93 1056 1534
Fragments arranged by size:
2211 1534 1056 644 594 578 510 464
435 372 215 171 93 84 79 22
BmgBI CAC'GTC
Cuts at: 2886 2886
Size: 9062
BmrI ACTGGGnnnn_n'
Cuts at: 215 619 1773 1834 2429 2511 4356 6274 6355
Size: 404 1154 61 595 82 1845 1918 81
Cuts at: 6355 6690 8834 215
Size: 335 2144 443
Fragments arranged by size:
2144 1918 1845 1154 595 443 404 335
82 81 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7429 7429
Size: 9062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 511 1066 1750 1864 2047 2371 2893 3019
Size: 336 555 684 114 183 324 522 126
Cuts at: 3019 3052 3103 3145 3355 3388 3439 3502 3640
Size: 33 51 42 210 33 51 63 138
Cuts at: 3640 3703 3724 3808 3925 4432 4564 4570 4633
Size: 63 21 84 117 507 132 6 63
Cuts at: 4633 4675 4759 4852 4906 4927 4933 4963 5149
Size: 42 84 93 54 21 6 30 186
Cuts at: 5149 5296 5329 5350 5464 5617 5632 5719 5740
Size: 147 33 21 114 153 15 87 21
Cuts at: 5740 175
Size: 3497
Fragments arranged by size:
3497 684 555 522 507 336 324 210
186 183 153 147 138 132 126 117
114 114 93 87 84 84 63 63
63 54 51 51 42 42 33 33
33 30 21 21 21 21 15 6
6
Bpu10I CC'TnA_GC
Cuts at: 2789 4307 4563 5507 2789
Size: 1518 256 944 6344
Fragments arranged by size:
6344 1518 944 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 2671 3088 3449 3478 4732 5080 5188 5665
Size: 1761 417 361 29 1254 348 108 477
Cuts at: 5665 6238 7117 7609 7907 910
Size: 573 879 492 298 2065
Fragments arranged by size:
2065 1761 1254 879 573 492 477 417
361 348 298 108 29
BsaI GGTCTCn'nnnn_
Cuts at: 409 2047 4912 8766 409
Size: 1638 2865 3854 705
Fragments arranged by size:
3854 2865 1638 705
BsaAI yAC'GTr
Cuts at: 366 1473 6415 366
Size: 1107 4942 3013
Fragments arranged by size:
4942 3013 1107
BsaBI GATnn'nnATC
Cuts at: 6 2362 6576 8613 6
Size: 2356 4214 2037 455
Fragments arranged by size:
4214 2356 2037 455
BsaHI Gr'CG_yC
Cuts at: 359 1190 1304 1877 2567 4203 6032 6493 6741
Size: 831 114 573 690 1636 1829 461 248
Cuts at: 6741 7443 8939 359
Size: 702 1496 482
Fragments arranged by size:
1829 1636 1496 831 702 690 573 482
461 248 114
BsaWI w'CCGG_w
Cuts at: 760 2360 4973 6251 6772 7450 7724 7871 8282
Size: 1600 2613 1278 521 678 274 147 411
Cuts at: 8282 760
Size: 1540
Fragments arranged by size:
2613 1600 1540 1278 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3076 3106 4123 4153 3076
Size: 30 1017 30 7985
Fragments arranged by size:
7985 1017 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 199 954 1237 1366 1417 1753 1861 2806
Size: 3 755 283 129 51 336 108 945
Cuts at: 2806 2809 2890 2974 3055 3058 3307 3499 3616
Size: 3 81 84 81 3 249 192 117
Cuts at: 3616 3628 3700 3721 3805 4060 4144 4228 4312
Size: 12 72 21 84 255 84 84 84
Cuts at: 4312 4315 4383 4420 4429 4471 4648 4651 4735
Size: 3 68 37 9 42 177 3 84
Cuts at: 4735 4789 4903 4930 5011 5224 5245 5326 5494
Size: 54 114 27 81 213 21 81 168
Cuts at: 5494 5524 5569 5662 5833 5917 5989 196
Size: 30 45 93 171 84 72 3269
Fragments arranged by size:
3269 945 755 336 283 255 249 213
192 177 171 168 129 117 114 108
93 84 84 84 84 84 84 84
81 81 81 81 72 72 68 54
51 45 42 37 30 27 21 21
12 9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 1630 2198 2490 2554 2667 3765 3861 5139 5459
Size: 568 292 64 113 1098 96 1278 320
Cuts at: 5459 5951 6487 7245 7822 8318 1630
Size: 492 536 758 577 496 2374
Fragments arranged by size:
2374 1278 1098 758 577 568 536 496
492 320 292 113 96 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1297 2188 3013 3683 4007 4273 4865 5471
Size: 777 891 825 670 324 266 592 606
Cuts at: 5471 5710 520
Size: 239 3872
Fragments arranged by size:
3872 891 825 777 670 606 592 324
266 239
BsiEI CG_ry'CG
Cuts at: 104 2852 6604 6650 7858 104
Size: 2748 3752 46 1208 1308
Fragments arranged by size:
3752 2748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 308 1229 2582 3097 3104 4489 4697 4949 5255
Size: 921 1353 515 7 1385 208 252 306
Cuts at: 5255 5586 5633 5804 6076 6854 7044 7836 308
Size: 331 47 171 272 778 190 792 1534
Fragments arranged by size:
1534 1385 1353 921 792 778 515 331
306 272 252 208 190 171 47 7
BsmI GAATG_Cn'
Cuts at: 989 1858 3364 5403 989
Size: 869 1506 2039 4648
Fragments arranged by size:
4648 2039 1506 869
BsmBI CGTCTCn'nnnn_
Cuts at: 830 2872 4735 830
Size: 2042 1863 5157
Fragments arranged by size:
5157 2042 1863
Bsp1286I G_dGCh'C
Cuts at: 308 886 1229 1521 2125 2141 2497 2582 3097
Size: 578 343 292 604 16 356 85 515
Cuts at: 3097 3104 3561 3645 4239 4489 4611 4690 4697
Size: 7 457 84 594 250 122 79 7
Cuts at: 4697 4905 4949 5076 5255 5586 5633 5804 6021
Size: 208 44 127 179 331 47 171 217
Cuts at: 6021 6043 6076 6687 6780 6854 7044 7106 7836
Size: 22 33 611 93 74 190 62 730
Cuts at: 7836 308
Size: 1534
Fragments arranged by size:
1534 730 611 604 594 578 515 457
356 343 331 292 250 217 208 190
179 171 127 122 93 85 84 79
74 62 47 44 33 22 16 7
7
BspEI T'CCGG_A
Cuts at: 2360 8282 2360
Size: 5922 3140
BspHI T'CATG_A
Cuts at: 8238 8238
Size: 9062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 551 1488 1799 2246 3290 3861 4775 5676 6112
Size: 937 311 447 1044 571 914 901 436
Cuts at: 6112 6326 6628 7009 551
Size: 214 302 381 2604
Fragments arranged by size:
2604 1044 937 914 901 571 447 436
381 311 302 214
BsrBI CCG'CTC
Cuts at: 50 671 3027 3402 3630 3699 4554 5468 5979
Size: 621 2356 375 228 69 855 914 511
Cuts at: 5979 7354 7408 8892 9057 50
Size: 1375 54 1484 165 55
Fragments arranged by size:
2356 1484 1375 914 855 621 511 375
228 165 69 55 54
BsrDI GCAATG_nn'
Cuts at: 2346 5085 6974 2346
Size: 2739 1889 4434
Fragments arranged by size:
4434 2739 1889
BsrFI r'CCGG_y
Cuts at: 775 1541 3071 5348 7060 7241 7450 775
Size: 766 1530 2277 1712 181 209 2387
Fragments arranged by size:
2387 2277 1712 1530 766 209 181
BssHII G'CGCG_C
Cuts at: 3308 3308
Size: 9062
BssSI C'ACGA_G
Cuts at: 3808 4294 4717 5257 5956 7333 7691 3808
Size: 486 423 540 699 1377 358 5179
Fragments arranged by size:
5179 1377 699 540 486 423 358
BstAPI GCAn_nnn'nTGC
Cuts at: 543 1140 3891 4260 4332 4573 5622 6439 543
Size: 597 2751 369 72 241 1049 817 3166
Fragments arranged by size:
3166 2751 1049 817 597 369 241 72
BstBI TT'CG_AA
Cuts at: 1661 7423 1661
Size: 5762 3300
BstEII G'GTnAC_C
Cuts at: 3277 3424 5113 3277
Size: 147 1689 7226
Fragments arranged by size:
7226 1689 147
BstXI CCAn_nnnn'nTGG
Cuts at: 1075 1075
Size: 9062
BstYI r'GATC_y
Cuts at: 1 1106 1640 2040 2357 2363 4871 4934 6087
Size: 1105 534 400 317 6 2508 63 1153
Cuts at: 6087 6554 6577 6912 7158 8170 8256 8268 8614
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8614 1
Size: 449
Fragments arranged by size:
2508 1153 1105 1012 534 467 449 400
346 335 317 246 86 63 23 12
6
Bsu36I CC'TnA_GG
Cuts at: 949 5011 949
Size: 4062 5000
BtgI C'CryG_G
Cuts at: 150 316 410 920 1022 1199 1247 2057 2631
Size: 166 94 510 102 177 48 810 574
Cuts at: 2631 3725 4161 4677 5670 150
Size: 1094 436 516 993 3542
Fragments arranged by size:
3542 1094 993 810 574 516 510 436
177 166 102 94 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1215 1611 7012 7193 7356 1215
Size: 396 5401 181 163 2921
Fragments arranged by size:
5401 2921 396 181 163
BtsI GCAGTG_nn'
Cuts at: 227 1422 3826 4435 6305 227
Size: 1195 2404 609 1870 2984
Fragments arranged by size:
2984 2404 1870 1195 609
DraI TTT'AAA
Cuts at: 6061 6061
Size: 9062
DraIII CAC_nnn'GTG
Cuts at: 4251 4251
Size: 9062
DrdI GACnn_nn'nnGTC
Cuts at: 3444 6768 7626 3444
Size: 3324 858 4880
Fragments arranged by size:
4880 3324 858
EaeI y'GGCC_r
Cuts at: 500 1523 2529 2597 2634 2849 3062 3137 4175
Size: 1023 1006 68 37 215 213 75 1038
Cuts at: 4175 4713 5540 5549 5933 6647 6821 7212 7239
Size: 538 827 9 384 714 174 391 27
Cuts at: 7239 8584 500
Size: 1345 978
Fragments arranged by size:
1345 1038 1023 1006 978 827 714 538
391 384 215 213 174 75 68 37
27 9
EagI C'GGCC_G
Cuts at: 2849 6647 2849
Size: 3798 5264
EarI CTCTTCn'nnn_
Cuts at: 595 2570 3188 3605 3910 4187 4702 4810 4858
Size: 1975 618 417 305 277 515 108 48
Cuts at: 4858 7085 7295 595
Size: 2227 210 2362
Fragments arranged by size:
2362 2227 1975 618 515 417 305 277
210 108 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 718 1375 3613 3775 3859 3871 4081 5464
Size: 453 657 2238 162 84 12 210 1383
Cuts at: 5464 5923 6007 7270 7580 7726 8872 265
Size: 459 84 1263 310 146 1146 455
Fragments arranged by size:
2238 1383 1263 1146 657 459 455 453
310 210 162 146 84 84 12
EcoICRI GAG'CTC
Cuts at: 1227 2580 3102 4695 4947 5253 5631 5802 6074
Size: 1353 522 1593 252 306 378 171 272
Cuts at: 6074 1227
Size: 4215
Fragments arranged by size:
4215 1593 1353 522 378 306 272 252
171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 511 1066 1248 1750 1864 2047 2371 2893
Size: 336 555 182 502 114 183 324 522
Cuts at: 2893 3019 3052 3103 3145 3355 3388 3439 3451
Size: 126 33 51 42 210 33 51 12
Cuts at: 3451 3502 3640 3703 3724 3808 3925 4432 4564
Size: 51 138 63 21 84 117 507 132
Cuts at: 4564 4570 4633 4675 4759 4852 4906 4927 4933
Size: 6 63 42 84 93 54 21 6
Cuts at: 4933 4963 5149 5296 5329 5350 5464 5581 5617
Size: 30 186 147 33 21 114 117 36
Cuts at: 5617 5632 5719 5740 6887 7319 8066 8334 175
Size: 15 87 21 1147 432 747 268 903
Fragments arranged by size:
1147 903 747 555 522 507 502 432
336 324 268 210 186 183 182 147
138 132 126 117 117 114 114 93
87 84 84 63 63 54 51 51
51 42 42 36 33 33 33 30
21 21 21 21 15 12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 277 520 1846 2599 3370 4126 4771 5332 5647
Size: 243 1326 753 771 756 645 561 315
Cuts at: 5647 277
Size: 3692
Fragments arranged by size:
3692 1326 771 756 753 645 561 315
243
EcoO109I rG'GnC_Cy
Cuts at: 1422 2346 3270 3351 3557 4170 4901 4902 5760
Size: 924 924 81 206 613 731 1 858
Cuts at: 5760 5850 6040 6201 1422
Size: 90 190 161 4283
Fragments arranged by size:
4283 924 924 858 731 613 206 190
161 90 81 1
EcoRI G'AATT_C
Cuts at: 6066 6066
Size: 9062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2562 7081 2562
Size: 4519 4543
FspI TGC'GCA
Cuts at: 730 3388 3928 5776 6843 730
Size: 2658 540 1848 1067 2949
Fragments arranged by size:
2949 2658 1848 1067 540
HaeII r_GCGC'y
Cuts at: 555 669 1307 2595 2664 2842 2865 2967 3802
Size: 114 638 1288 69 178 23 102 835
Cuts at: 3802 4206 4852 5089 5283 5391 5790 5895 6496
Size: 404 646 237 194 108 399 105 601
Cuts at: 6496 6744 7766 8745 555
Size: 248 1022 979 872
Fragments arranged by size:
1288 1022 979 872 835 646 638 601
404 399 248 237 194 178 114 108
105 102 69 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 730 762 949 981 6904 6934 6936
Size: 32 331 32 187 32 5923 30 2
Cuts at: 6936 6966 367
Size: 30 2463
Fragments arranged by size:
5923 2463 331 187 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 789 6101 789
Size: 5312 3750
HpaI GTT'AAC
Cuts at: 789 789
Size: 9062
KasI G'GCGC_C
Cuts at: 1303 4202 6492 6740 1303
Size: 2899 2290 248 3625
Fragments arranged by size:
3625 2899 2290 248
KpnI G_GTAC'C
Cuts at: 2421 6082 2421
Size: 3661 5401
MluI A'CGCG_T
Cuts at: 8495 8495
Size: 9062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 365 4475 7733 7917 365
Size: 4110 3258 184 1510
Fragments arranged by size:
4110 3258 1510 184
MscI TGG'CCA
Cuts at: 502 1525 2531 2599 3139 5935 6823 502
Size: 1023 1006 68 540 2796 888 2741
Fragments arranged by size:
2796 2741 1023 1006 888 540 68
MslI CAynn'nnrTG
Cuts at: 152 221 232 502 692 1198 1723 2458 2539
Size: 69 11 270 190 506 525 735 81
Cuts at: 2539 3934 4271 7051 7178 152
Size: 1395 337 2780 127 2036
Fragments arranged by size:
2780 2036 1395 735 525 506 337 270
190 127 81 69 11
MspA1I CmG'CkG
Cuts at: 563 1024 1347 1668 2202 2267 2331 2415 2769
Size: 461 323 321 534 65 64 84 354
Cuts at: 2769 2925 2997 3480 3522 3727 3888 4056 4062
Size: 156 72 483 42 205 161 168 6
Cuts at: 4062 4155 4329 4570 4593 4647 4941 5139 5238
Size: 93 174 241 23 54 294 198 99
Cuts at: 5238 5248 5463 6173 6487 6847 7860 8105 563
Size: 10 215 710 314 360 1013 245 1520
Fragments arranged by size:
1520 1013 710 534 483 461 360 354
323 321 314 294 245 241 215 205
198 174 168 161 156 99 93 84
72 65 64 54 42 23 10 6
NaeI GCC'GGC
Cuts at: 3073 5350 7243 3073
Size: 2277 1893 4892
Fragments arranged by size:
4892 2277 1893
NarI GG'CG_CC
Cuts at: 1304 4203 6493 6741 1304
Size: 2899 2290 248 3625
Fragments arranged by size:
3625 2899 2290 248
NcoI C'CATG_G
Cuts at: 316 920 1247 316
Size: 604 327 8131
Fragments arranged by size:
8131 604 327
NgoMIV G'CCGG_C
Cuts at: 3071 5348 7241 3071
Size: 2277 1893 4892
Fragments arranged by size:
4892 2277 1893
NsiI A_TGCA'T
Cuts at: 1858 1858
Size: 9062
NspI r_CATG'y
Cuts at: 726 1143 1722 1860 5847 6115 6615 7522 726
Size: 417 579 138 3987 268 500 907 2266
Fragments arranged by size:
3987 2266 907 579 500 417 268 138
PasI CC'CwG_GG
Cuts at: 831 2296 3273 6036 831
Size: 1465 977 2763 3857
Fragments arranged by size:
3857 2763 1465 977
PciI A'CATG_T
Cuts at: 1718 7518 1718
Size: 5800 3262
PflMI CCAn_nnn'nTGG
Cuts at: 864 1248 1491 1581 2595 2699 2871 4188 4830
Size: 384 243 90 1014 104 172 1317 642
Cuts at: 4830 864
Size: 5096
Fragments arranged by size:
5096 1317 1014 642 384 243 172 104
90
PfoI T'CCnGG_A
Cuts at: 1043 1841 2348 2444 2570 4823 1043
Size: 798 507 96 126 2253 5282
Fragments arranged by size:
5282 2253 798 507 126 96
PmeI GTTT'AAAC
Cuts at: 6061 6061
Size: 9062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 511 543 8201 8233 511
Size: 32 7658 32 1340
Fragments arranged by size:
7658 1340 32 32
PpuMI rG'GwC_Cy
Cuts at: 1422 2346 3351 5850 1422
Size: 924 1005 2499 4634
Fragments arranged by size:
4634 2499 1005 924
PshAI GACnn'nnGTC
Cuts at: 2059 2884 2059
Size: 825 8237
PspOMI G'GGCC_C
Cuts at: 3557 3641 4901 5072 6039 3557
Size: 84 1260 171 967 6580
Fragments arranged by size:
6580 1260 967 171 84
PstI C_TGCA'G
Cuts at: 461 1136 1183 1796 2621 2924 2930 3287 3590
Size: 675 47 613 825 303 6 357 303
Cuts at: 3590 3887 4055 4328 4448 4772 5048 5138 5870
Size: 297 168 273 120 324 276 90 732
Cuts at: 5870 6109 6794 461
Size: 239 685 2729
Fragments arranged by size:
2729 825 732 685 675 613 357 324
303 303 297 276 273 239 168 120
90 47 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9062
PvuII CAG'CTG
Cuts at: 1668 2202 2331 2925 2997 3480 3522 4062 4155
Size: 534 129 594 72 483 42 540 93
Cuts at: 4155 4570 4941 5139 5248 5463 6487 6847 1668
Size: 415 371 198 109 215 1024 360 3883
Fragments arranged by size:
3883 1024 594 540 534 483 415 371
360 215 198 129 109 93 72 42
RsrII CG'GwC_CG
Cuts at: 7257 7257
Size: 9062
SacI G_AGCT'C
Cuts at: 1229 2582 3104 4697 4949 5255 5633 5804 6076
Size: 1353 522 1593 252 306 378 171 272
Cuts at: 6076 1229
Size: 4215
Fragments arranged by size:
4215 1593 1353 522 378 306 272 252
171
SacII CC_GC'GG
Cuts at: 1025 3728 1025
Size: 2703 6359
SalI G'TCGA_C
Cuts at: 6099 6099
Size: 9062
SapI GCTCTTCn'nnn_
Cuts at: 2570 4702 4810 7085 7295 2570
Size: 2132 108 2275 210 4337
Fragments arranged by size:
4337 2275 2132 210 108
SbfI CC_TGCA'GG
Cuts at: 3287 4772 5048 6109 3287
Size: 1485 276 1061 6240
Fragments arranged by size:
6240 1485 1061 276
ScaI AGT'ACT
Cuts at: 811 8579 811
Size: 7768 1294
SexAI A'CCwGG_T
Cuts at: 4796 5852 4796
Size: 1056 8006
SfcI C'TryA_G
Cuts at: 33 457 1132 1179 1792 2617 2920 2926 3283
Size: 424 675 47 613 825 303 6 357
Cuts at: 3283 3586 3883 4051 4093 4324 4444 4516 4768
Size: 303 297 168 42 231 120 72 252
Cuts at: 4768 5044 5134 5866 6105 6790 7783 7974 33
Size: 276 90 732 239 685 993 191 1121
Fragments arranged by size:
1121 993 825 732 685 675 613 424
357 303 303 297 276 252 239 231
191 168 120 90 72 47 42 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 5167 5524 5548 5167
Size: 357 24 8681
Fragments arranged by size:
8681 357 24
SfoI GGC'GCC
Cuts at: 1305 4204 6494 6742 1305
Size: 2899 2290 248 3625
Fragments arranged by size:
3625 2899 2290 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9062
SgrAI Cr'CCGG_yG
Cuts at: 3071 3071
Size: 9062
SmaI CCC'GGG
Cuts at: 1450 3640 6028 6084 1450
Size: 2190 2388 56 4428
Fragments arranged by size:
4428 2388 2190 56
SmlI C'TyrA_G
Cuts at: 889 2650 3103 3464 3493 4747 5095 5203 5644
Size: 1761 453 361 29 1254 348 108 441
Cuts at: 5644 5764 6217 7132 7624 7886 889
Size: 120 453 915 492 262 2065
Fragments arranged by size:
2065 1761 1254 915 492 453 453 441
361 348 262 120 108 29
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9062
SphI G_CATG'C
Cuts at: 5847 6115 6615 5847
Size: 268 500 8294
Fragments arranged by size:
8294 500 268
SrfI GCCC'GGGC
Cuts at: 3640 3640
Size: 9062
StuI AGG'CCT
Cuts at: 526 587 928 1436 1870 5050 5420 526
Size: 61 341 508 434 3180 370 4168
Fragments arranged by size:
4168 3180 508 434 370 341 61
StyI C'CwwG_G
Cuts at: 172 316 920 1247 1257 1478 1526 1865 2373
Size: 144 604 327 10 221 48 339 508
Cuts at: 2373 2537 2600 2987 3233 3977 4340 5705 6196
Size: 164 63 387 246 744 363 1365 491
Cuts at: 6196 6550 172
Size: 354 2684
Fragments arranged by size:
2684 1365 744 604 508 491 387 363
354 339 327 246 221 164 144 63
48 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 330 1938 2530 2911 3265 4417 6645 6778 7237
Size: 1608 592 381 354 1152 2228 133 459
Cuts at: 7237 7446 330
Size: 209 1946
Fragments arranged by size:
2228 1946 1608 1152 592 459 381 354
209 133
TatI w'GTAC_w
Cuts at: 809 1742 8577 809
Size: 933 6835 1294
Fragments arranged by size:
6835 1294 933
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1814 1846 1913 1945 2694 2726 3076 3108 4123
Size: 32 67 32 749 32 350 32 1015
Cuts at: 4123 4155 8579 8611 1814
Size: 32 4424 32 2265
Fragments arranged by size:
4424 2265 1015 749 350 67 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 2193 4524 6859 1765
Size: 428 2331 2335 3968
Fragments arranged by size:
3968 2335 2331 428
XbaI T'CTAG_A
Cuts at: 6093 6093
Size: 9062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 918 2524 2994 3330 3414 4038 4236 4353
Size: 684 1606 470 336 84 624 198 117
Cuts at: 4353 4428 4848 5928 6557 234
Size: 75 420 1080 629 2739
Fragments arranged by size:
2739 1606 1080 684 629 624 470 420
336 198 117 84 75
XmaI C'CCGG_G
Cuts at: 1448 3638 6026 6082 1448
Size: 2190 2388 56 4428
Fragments arranged by size:
4428 2388 2190 56
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AlfI AscI BaeI BmtI BsiWI BsrGI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII MfeI NdeI
NheI NotI NruI PacI PmlI PsiI PspXI PsrI
SanDI SnaBI SspI SwaI XhoI XmnI ZraI