Restriction map for
pF1KA1516
(Circular) (Six-base) MAPSORT of: pF1KA1516.seq Check: 868 from: 1 to: 10019
pF1KA1516 10019 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6810 6810
Size: 10019
AatII G_ACGT'C
Cuts at: 3601 5795 3601
Size: 2194 7825
AccI GT'mk_AC
Cuts at: 1129 1766 2939 5789 7057 1129
Size: 637 1173 2850 1268 4091
Fragments arranged by size:
4091 2850 1268 1173 637
Acc65I G'GTAC_C
Cuts at: 7035 7035
Size: 10019
AclI AA'CG_TT
Cuts at: 9859 9859
Size: 10019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 2149 2257 2743 3025 3087 3826 3892 4072
Size: 2012 108 486 282 62 739 66 180
Cuts at: 4072 4936 5989 7844 8276 9023 9291 137
Size: 864 1053 1855 432 747 268 865
Fragments arranged by size:
2012 1855 1053 865 864 747 739 486
432 282 268 180 108 66 62
AflII C'TTAA_G
Cuts at: 6805 6805
Size: 10019
AflIII A'CryG_T
Cuts at: 2182 7371 8475 9452 2182
Size: 5189 1104 977 2749
Fragments arranged by size:
5189 2749 1104 977
AgeI A'CCGG_T
Cuts at: 1507 8407 1507
Size: 6900 3119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1774 3852 1774
Size: 2078 7941
AlwNI CAG_nnn'CTG
Cuts at: 133 400 2688 3016 3174 5433 5460 5880 6231
Size: 267 2288 328 158 2259 27 420 351
Cuts at: 6231 7324 8891 133
Size: 1093 1567 1261
Fragments arranged by size:
2288 2259 1567 1261 1093 420 351 328
267 158 27
ApaLI G'TGCA_C
Cuts at: 2677 8789 2677
Size: 6112 3907
ApoI r'AATT_y
Cuts at: 524 560 2385 2863 3148 3757 4242 4604 4642
Size: 36 1825 478 285 609 485 362 38
Cuts at: 4642 5050 5134 6078 6239 6383 6796 7023 9372
Size: 408 84 944 161 144 413 227 2349
Cuts at: 9372 9628 524
Size: 256 915
Fragments arranged by size:
2349 1825 944 915 609 485 478 413
408 362 285 256 227 161 144 84
38 36
AseI AT'TA_AT
Cuts at: 20 3485 6203 20
Size: 3465 2718 3836
Fragments arranged by size:
3836 3465 2718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10019
AvaI C'yCGr_G
Cuts at: 6833 6905 7039 6833
Size: 72 134 9813
Fragments arranged by size:
9813 134 72
BamHI G'GATC_C
Cuts at: 1573 5706 7044 1573
Size: 4133 1338 4548
Fragments arranged by size:
4548 4133 1338
BanI G'GyrC_C
Cuts at: 2263 3075 6211 7035 7449 7697 7732 2263
Size: 812 3136 824 414 248 35 4550
Fragments arranged by size:
4550 3136 824 812 414 248 35
BanII G_rGCy'C
Cuts at: 1819 1924 2246 2642 3555 3655 5236 6185 6502
Size: 105 322 396 913 100 1581 949 317
Cuts at: 6502 7033 8063 1819
Size: 531 1030 3775
Fragments arranged by size:
3775 1581 1030 949 913 531 396 322
317 105 100
BbeI G_GCGC'C
Cuts at: 2267 7453 7701 2267
Size: 5186 248 4585
Fragments arranged by size:
5186 4585 248
BbsI GAAGACnn'nnnn_
Cuts at: 509 1049 3629 4922 6715 509
Size: 540 2580 1293 1793 3813
Fragments arranged by size:
3813 2580 1793 1293 540
BbvCI CC'TCA_GC
Cuts at: 497 4147 4583 497
Size: 3650 436 5933
Fragments arranged by size:
5933 3650 436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4387 4421 5769 5803 6814 6843 6848 6877 4387
Size: 34 1348 34 1011 29 5 29 7529
Fragments arranged by size:
7529 1348 1011 34 34 29 29 5
BciVI GTATCCnnnnn_n'
Cuts at: 1319 5525 6406 6958 7917 8678 10019 1319
Size: 4206 881 552 959 761 1341 1319
Fragments arranged by size:
4206 1341 1319 959 881 761 552
BclI T'GATC_A
Cuts at: 620 867 5061 7539 620
Size: 247 4194 2478 3100
Fragments arranged by size:
4194 3100 2478 247
BfrBI ATG'CAT
Cuts at: 5161 5161
Size: 10019
BglI GCCn_nnn'nGGC
Cuts at: 178 1610 2954 178
Size: 1432 1344 7243
Fragments arranged by size:
7243 1432 1344
BglII A'GATC_T
Cuts at: 1893 3921 5691 5901 7534 9571 1893
Size: 2028 1770 210 1633 2037 2341
Fragments arranged by size:
2341 2037 2028 1770 1633 210
BlpI GC'TnA_GC
Cuts at: 2852 7131 2852
Size: 4279 5740
Bme1580I G_kGCm'C
Cuts at: 1801 2190 2681 3836 5755 7644 7737 8793 1801
Size: 389 491 1155 1919 1889 93 1056 3027
Fragments arranged by size:
3027 1919 1889 1155 1056 491 389 93
BmgBI CAC'GTC
Cuts at: 1532 5336 1532
Size: 3804 6215
BmrI ACTGGGnnnn_n'
Cuts at: 168 1296 2468 2884 3001 4052 5050 5750 7231
Size: 1128 1172 416 117 1051 998 700 1481
Cuts at: 7231 7312 7647 9791 168
Size: 81 335 2144 396
Fragments arranged by size:
2144 1481 1172 1128 1051 998 700 416
396 335 117 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1938 4076 8386 1938
Size: 2138 4310 3571
Fragments arranged by size:
4310 3571 2138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 2475 2737 5618 6527 6972 2470
Size: 5 262 2881 909 445 5517
Fragments arranged by size:
5517 2881 909 445 262 5
Bpu10I CC'TnA_GC
Cuts at: 497 4147 4583 6643 497
Size: 3650 436 2060 3873
Fragments arranged by size:
3873 3650 2060 436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3461 6516 6859 7195 8074 8566 8864 3461
Size: 3055 343 336 879 492 298 4616
Fragments arranged by size:
4616 3055 879 492 343 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 210 2107 2278 9723 210
Size: 1897 171 7445 506
Fragments arranged by size:
7445 1897 506 171
BsaAI yAC'GTr
Cuts at: 2185 2655 7372 2185
Size: 470 4717 4832
Fragments arranged by size:
4832 4717 470
BsaBI GATnn'nnATC
Cuts at: 6 4450 7533 9570 6
Size: 4444 3083 2037 455
Fragments arranged by size:
4444 3083 2037 455
BsaHI Gr'CG_yC
Cuts at: 2264 3598 4747 5792 7450 7698 8400 9896 2264
Size: 1334 1149 1045 1658 248 702 1496 2387
Fragments arranged by size:
2387 1658 1496 1334 1149 1045 702 248
BsaWI w'CCGG_w
Cuts at: 1507 2269 2429 7208 7729 8407 8681 8828 9239
Size: 762 160 4779 521 678 274 147 411
Cuts at: 9239 1507
Size: 2287
Fragments arranged by size:
4779 2287 762 678 521 411 274 160
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1080 1110 1272 1302 2251 2281 2383 2413 3588
Size: 30 162 30 949 30 102 30 1175
Cuts at: 3588 3618 3660 3690 6745 6775 1080
Size: 30 42 30 3055 30 4324
Fragments arranged by size:
4324 3055 1175 949 162 102 42 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 790 1978 2136 2476 4876 6385 6520 6523 6976
Size: 1188 158 340 2400 1509 135 3 453
Cuts at: 6976 790
Size: 3833
Fragments arranged by size:
3833 2400 1509 1188 453 340 158 135
3
BseYI C'CCAG_C
Cuts at: 1386 1654 2144 2808 3283 3571 4427 5438 6440
Size: 268 490 664 475 288 856 1011 1002
Cuts at: 6440 7444 8202 8779 9275 1386
Size: 1004 758 577 496 2130
Fragments arranged by size:
2130 1011 1004 1002 856 758 664 577
496 490 475 288 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 4113 4819 6572 6809 6844 2176
Size: 1937 706 1753 237 35 5351
Fragments arranged by size:
5351 1937 1753 706 237 35
BsiEI CG_ry'CG
Cuts at: 104 7561 7607 8815 104
Size: 7457 46 1208 1308
Fragments arranged by size:
7457 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 642 1819 1880 1924 2246 2642 2681 3276 4057
Size: 1177 61 44 322 396 39 595 781
Cuts at: 4057 6502 7033 7811 8001 8793 642
Size: 2445 531 778 190 792 1868
Fragments arranged by size:
2445 1868 1177 792 781 778 595 531
396 322 190 61 44 39
BsmI GAATG_Cn'
Cuts at: 1813 3312 3516 4824 5288 1813
Size: 1499 204 1308 464 6544
Fragments arranged by size:
6544 1499 1308 464 204
BsmBI CGTCTCn'nnnn_
Cuts at: 4430 4430
Size: 10019
Bsp1286I G_dGCh'C
Cuts at: 642 1801 1819 1880 1924 2190 2246 2642 2681
Size: 1159 18 61 44 266 56 396 39
Cuts at: 2681 3276 3555 3655 3836 4057 5236 5755 6185
Size: 595 279 100 181 221 1179 519 430
Cuts at: 6185 6502 7033 7644 7737 7811 8001 8063 8793
Size: 317 531 611 93 74 190 62 730
Cuts at: 8793 642
Size: 1868
Fragments arranged by size:
1868 1179 1159 730 611 595 531 519
430 396 317 279 266 221 190 181
100 93 74 62 61 56 44 39
18
BspEI T'CCGG_A
Cuts at: 2429 9239 2429
Size: 6810 3209
BspHI T'CATG_A
Cuts at: 2276 3380 9195 2276
Size: 1104 5815 3100
Fragments arranged by size:
5815 3100 1104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4550 4842 5408 6810 7069 7283 7585 7966 4550
Size: 292 566 1402 259 214 302 381 6603
Fragments arranged by size:
6603 1402 566 381 302 292 259 214
BsrBI CCG'CTC
Cuts at: 50 8311 8365 9849 10014 50
Size: 8261 54 1484 165 55
Fragments arranged by size:
8261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1444 3025 4010 4576 5597 7931 1444
Size: 1581 985 566 1021 2334 3532
Fragments arranged by size:
3532 2334 1581 1021 985 566
BsrFI r'CCGG_y
Cuts at: 1507 8017 8198 8407 1507
Size: 6510 181 209 3119
Fragments arranged by size:
6510 3119 209 181
BsrGI T'GTAC_A
Cuts at: 482 482
Size: 10019
BssSI C'ACGA_G
Cuts at: 4105 8290 8648 4105
Size: 4185 358 5476
Fragments arranged by size:
5476 4185 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3174 3307 6231 7396 3174
Size: 133 2924 1165 5797
Fragments arranged by size:
5797 2924 1165 133
BstBI TT'CG_AA
Cuts at: 5897 8380 5897
Size: 2483 7536
BstXI CCAn_nnnn'nTGG
Cuts at: 451 1536 5642 451
Size: 1085 4106 4828
Fragments arranged by size:
4828 4106 1085
BstYI r'GATC_y
Cuts at: 1 1357 1573 1893 2916 3810 3921 4484 5691
Size: 1356 216 320 1023 894 111 563 1207
Cuts at: 5691 5706 5901 7044 7511 7534 7869 8115 9127
Size: 15 195 1143 467 23 335 246 1012
Cuts at: 9127 9213 9225 9571 1
Size: 86 12 346 449
Fragments arranged by size:
1356 1207 1143 1023 1012 894 563 467
449 346 335 320 246 216 195 111
86 23 15 12
BstZ17I GTA'TAC
Cuts at: 1130 2940 1130
Size: 1810 8209
Bsu36I CC'TnA_GG
Cuts at: 2059 2059
Size: 10019
BtgI C'CryG_G
Cuts at: 587 1404 1958 2290 2628 2702 5526 587
Size: 817 554 332 338 74 2824 5080
Fragments arranged by size:
5080 2824 817 554 338 332 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3501 7969 8150 8313 3501
Size: 4468 181 163 5207
Fragments arranged by size:
5207 4468 181 163
BtsI GCAGTG_nn'
Cuts at: 868 1331 2933 3175 3836 4125 4527 6584 7262
Size: 463 1602 242 661 289 402 2057 678
Cuts at: 7262 868
Size: 3625
Fragments arranged by size:
3625 2057 1602 678 661 463 402 289
242
ClaI AT'CG_AT
Cuts at: 1676 4558 1676
Size: 2882 7137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 6760 6795 6760
Size: 35 9984
DraI TTT'AAA
Cuts at: 1053 2078 5582 5984 7018 1053
Size: 1025 3504 402 1034 4054
Fragments arranged by size:
4054 3504 1034 1025 402
DraIII CAC_nnn'GTG
Cuts at: 2973 2975 3072 3243 2973
Size: 2 97 171 9749
Fragments arranged by size:
9749 171 97 2
DrdI GACnn_nn'nnGTC
Cuts at: 7725 8583 7725
Size: 858 9161
EaeI y'GGCC_r
Cuts at: 827 1167 1622 2000 7604 7778 8169 8196 9541
Size: 340 455 378 5604 174 391 27 1345
Cuts at: 9541 827
Size: 1305
Fragments arranged by size:
5604 1345 1305 455 391 378 340 174
27
EagI C'GGCC_G
Cuts at: 7604 7604
Size: 10019
EarI CTCTTCn'nnn_
Cuts at: 1201 1326 3106 3543 4844 5499 6111 6362 6401
Size: 125 1780 437 1301 655 612 251 39
Cuts at: 6401 6452 6674 8042 8252 1201
Size: 51 222 1368 210 2968
Fragments arranged by size:
2968 1780 1368 1301 655 612 437 251
222 210 125 51 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3705 8227 8537 8683 9829 3705
Size: 4522 310 146 1146 3895
Fragments arranged by size:
4522 3895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1817 1922 2244 2640 6500 7031 1817
Size: 105 322 396 3860 531 4805
Fragments arranged by size:
4805 3860 531 396 322 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 2149 2257 2470 2475 2737 2743 3025 3087
Size: 2012 108 213 5 262 6 282 62
Cuts at: 3087 3826 3892 4072 4936 5618 5989 6527 6972
Size: 739 66 180 864 682 371 538 445
Cuts at: 6972 7844 8276 9023 9291 137
Size: 872 432 747 268 865
Fragments arranged by size:
2012 872 865 864 747 739 682 538
445 432 371 282 268 262 213 180
108 66 62 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2692 3199 2692
Size: 507 9512
EcoO109I rG'GnC_Cy
Cuts at: 1612 3575 3801 4389 6168 6738 7158 1612
Size: 1963 226 588 1779 570 420 4473
Fragments arranged by size:
4473 1963 1779 588 570 420 226
EcoRI G'AATT_C
Cuts at: 524 560 2385 7023 524
Size: 36 1825 4638 3520
Fragments arranged by size:
4638 3520 1825 36
EcoRV GAT'ATC
Cuts at: 4001 4758 5995 4001
Size: 757 1237 8025
Fragments arranged by size:
8025 1237 757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2845 4506 4732 8038 2845
Size: 1661 226 3306 4826
Fragments arranged by size:
4826 3306 1661 226
FspI TGC'GCA
Cuts at: 7800 7800
Size: 10019
HaeII r_GCGC'y
Cuts at: 2267 7453 7701 8723 9702 2267
Size: 5186 248 1022 979 2584
Fragments arranged by size:
5186 2584 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 429 461 1200 1232 1272 1304 1349 1381 2251
Size: 32 739 32 40 32 45 32 870
Cuts at: 2251 2283 2383 2415 2424 2456 3588 3620 4042
Size: 32 100 32 9 32 1132 32 422
Cuts at: 4042 4074 4873 4905 5233 5265 5550 5582 7861
Size: 32 799 32 328 32 285 32 2279
Cuts at: 7861 7891 7893 7923 429
Size: 30 2 30 2525
Fragments arranged by size:
2525 2279 1132 870 799 739 422 328
285 100 45 40 32 32 32 32
32 32 32 32 32 32 32 32
30 30 9 2
HincII GTy'rAC
Cuts at: 687 927 1256 1767 2409 3929 5150 5790 6893
Size: 240 329 511 642 1520 1221 640 1103
Cuts at: 6893 7058 687
Size: 165 3648
Fragments arranged by size:
3648 1520 1221 1103 642 640 511 329
240 165
HindIII A'AGCT_T
Cuts at: 2281 4948 5978 2281
Size: 2667 1030 6322
Fragments arranged by size:
6322 2667 1030
KasI G'GCGC_C
Cuts at: 2263 7449 7697 2263
Size: 5186 248 4585
Fragments arranged by size:
5186 4585 248
KpnI G_GTAC'C
Cuts at: 7039 7039
Size: 10019
MfeI C'AATT_G
Cuts at: 1145 3884 1145
Size: 2739 7280
MluI A'CGCG_T
Cuts at: 9452 9452
Size: 10019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1335 2528 5522 8690 8874 1335
Size: 1193 2994 3168 184 2480
Fragments arranged by size:
3168 2994 2480 1193 184
MscI TGG'CCA
Cuts at: 829 1169 1624 2002 7780 829
Size: 340 455 378 5778 3068
Fragments arranged by size:
5778 3068 455 378 340
MslI CAynn'nnrTG
Cuts at: 145 449 1723 4180 5582 8008 8135 145
Size: 304 1274 2457 1402 2426 127 2029
Fragments arranged by size:
2457 2426 2029 1402 1274 304 127
MspA1I CmG'CkG
Cuts at: 858 1654 2292 2744 2851 3107 3571 5457 5816
Size: 796 638 452 107 256 464 1886 359
Cuts at: 5816 5947 6009 6395 6735 7130 7444 7804 8817
Size: 131 62 386 340 395 314 360 1013
Cuts at: 8817 9062 858
Size: 245 1815
Fragments arranged by size:
1886 1815 1013 796 638 464 452 395
386 360 359 340 314 256 245 131
107 62
NaeI GCC'GGC
Cuts at: 8200 8200
Size: 10019
NarI GG'CG_CC
Cuts at: 2264 7450 7698 2264
Size: 5186 248 4585
Fragments arranged by size:
5186 4585 248
NcoI C'CATG_G
Cuts at: 587 1404 1958 2628 5526 587
Size: 817 554 670 2898 5080
Fragments arranged by size:
5080 2898 817 670 554
NgoMIV G'CCGG_C
Cuts at: 8198 8198
Size: 10019
NsiI A_TGCA'T
Cuts at: 5163 5163
Size: 10019
NspI r_CATG'y
Cuts at: 1999 3003 5805 7072 7572 8479 1999
Size: 1004 2802 1267 500 907 3539
Fragments arranged by size:
3539 2802 1267 1004 907 500
PasI CC'CwG_GG
Cuts at: 3342 5236 6172 3342
Size: 1894 936 7189
Fragments arranged by size:
7189 1894 936
PciI A'CATG_T
Cuts at: 8475 8475
Size: 10019
PflMI CCAn_nnn'nTGG
Cuts at: 766 2224 3665 766
Size: 1458 1441 7120
Fragments arranged by size:
7120 1458 1441
PfoI T'CCnGG_A
Cuts at: 2491 5139 2491
Size: 2648 7371
PmeI GTTT'AAAC
Cuts at: 7018 7018
Size: 10019
PmlI CAC'GTG
Cuts at: 2185 2185
Size: 10019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9158 9190 9158
Size: 32 9987
PpuMI rG'GwC_Cy
Cuts at: 3575 3801 4389 6168 6738 3575
Size: 226 588 1779 570 6856
Fragments arranged by size:
6856 1779 588 570 226
PshAI GACnn'nnGTC
Cuts at: 4200 4200
Size: 10019
PsiI TTA'TAA
Cuts at: 2533 4808 2533
Size: 2275 7744
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4277 4309 4277
Size: 32 9987
PstI C_TGCA'G
Cuts at: 186 863 1685 2696 4292 5715 6236 7066 7751
Size: 677 822 1011 1596 1423 521 830 685
Cuts at: 7751 186
Size: 2454
Fragments arranged by size:
2454 1596 1423 1011 830 822 685 677
521
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10019
PvuII CAG'CTG
Cuts at: 858 1654 2851 3571 5457 5816 6009 6395 7444
Size: 796 1197 720 1886 359 193 386 1049
Cuts at: 7444 7804 858
Size: 360 3073
Fragments arranged by size:
3073 1886 1197 1049 796 720 386 360
359 193
RsrII CG'GwC_CG
Cuts at: 8214 8214
Size: 10019
SacI G_AGCT'C
Cuts at: 1819 1924 2246 2642 6502 7033 1819
Size: 105 322 396 3860 531 4805
Fragments arranged by size:
4805 3860 531 396 322 105
SacII CC_GC'GG
Cuts at: 2293 2293
Size: 10019
SalI G'TCGA_C
Cuts at: 1765 5788 7056 1765
Size: 4023 1268 4728
Fragments arranged by size:
4728 4023 1268
SanDI GG'GwC_CC
Cuts at: 3575 6168 3575
Size: 2593 7426
SapI GCTCTTCn'nnn_
Cuts at: 3106 3543 8042 8252 3106
Size: 437 4499 210 4873
Fragments arranged by size:
4873 4499 437 210
SbfI CC_TGCA'GG
Cuts at: 7066 7066
Size: 10019
ScaI AGT'ACT
Cuts at: 1563 9536 1563
Size: 7973 2046
SfcI C'TryA_G
Cuts at: 33 182 859 1681 2692 4288 4380 5711 6232
Size: 149 677 822 1011 1596 92 1331 521
Cuts at: 6232 6633 7062 7747 8740 8931 33
Size: 401 429 685 993 191 1121
Fragments arranged by size:
1596 1331 1121 1011 993 822 685 677
521 429 401 191 149 92
SfoI GGC'GCC
Cuts at: 2265 7451 7699 2265
Size: 5186 248 4585
Fragments arranged by size:
5186 4585 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10019
SmaI CCC'GGG
Cuts at: 7041 7041
Size: 10019
SmlI C'TyrA_G
Cuts at: 3476 6495 6805 6833 6874 7174 8089 8581 8843
Size: 3019 310 28 41 300 915 492 262
Cuts at: 8843 3476
Size: 4652
Fragments arranged by size:
4652 3019 915 492 310 300 262 41
28
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10019
SphI G_CATG'C
Cuts at: 5805 7072 7572 5805
Size: 1267 500 8252
Fragments arranged by size:
8252 1267 500
SspI AAT'ATT
Cuts at: 219 6304 6628 219
Size: 6085 324 3610
Fragments arranged by size:
6085 3610 324
StuI AGG'CCT
Cuts at: 2620 2620
Size: 10019
StyI C'CwwG_G
Cuts at: 587 1404 1958 2628 2647 4116 5526 6215 6956
Size: 817 554 670 19 1469 1410 689 741
Cuts at: 6956 7153 7507 587
Size: 197 354 3099
Fragments arranged by size:
3099 1469 1410 817 741 689 670 554
354 197 19
SwaI ATTT'AAAT
Cuts at: 5582 5582
Size: 10019
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1917 6964 7602 7735 8194 8403 1917
Size: 5047 638 133 459 209 3533
Fragments arranged by size:
5047 3533 638 459 209 133
TatI w'GTAC_w
Cuts at: 482 1499 1561 2165 2835 9534 482
Size: 1017 62 604 670 6699 967
Fragments arranged by size:
6699 1017 967 670 604 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 493 525 6739 6771 9536 9568 493
Size: 32 6214 32 2765 32 944
Fragments arranged by size:
6214 2765 944 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7816 7816
Size: 10019
XbaI T'CTAG_A
Cuts at: 7050 7050
Size: 10019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1722 3662 4227 5763 6355 6996 7514 1722
Size: 1940 565 1536 592 641 518 4227
Fragments arranged by size:
4227 1940 1536 641 592 565 518
XhoI C'TCGA_G
Cuts at: 6833 6833
Size: 10019
XmaI C'CCGG_G
Cuts at: 7039 7039
Size: 10019
XmnI GAAnn'nnTTC
Cuts at: 528 2620 5274 5288 528
Size: 2092 2654 14 5259
Fragments arranged by size:
5259 2654 2092 14
ZraI GAC'GTC
Cuts at: 3599 5793 3599
Size: 2194 7825
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AhdI AleI AloI ApaI AscI AvrII BaeI
BmtI BsiWI BssHII BstEII FseI FspAI HpaI NdeI
NheI NotI NruI PacI PspOMI PspXI SexAI SfiI
SgrAI SnaBI SrfI