Restriction map for
pF1KA1522
(Circular) (Six-base) MAPSORT of: pF1KA1522.seq Check: 6196 from: 1 to: 6251
pF1KA1522 6251 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 860 3289 860
Size: 2429 3822
Acc65I G'GTAC_C
Cuts at: 895 1317 1638 3267 895
Size: 422 321 1629 3879
Fragments arranged by size:
3879 1629 422 321
AclI AA'CG_TT
Cuts at: 6091 6091
Size: 6251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2363 4076 4508 4861 5523 1465
Size: 898 1713 432 353 662 2193
Fragments arranged by size:
2193 1713 898 662 432 353
AflIII A'CryG_T
Cuts at: 3603 5403 5684 3603
Size: 1800 281 4170
Fragments arranged by size:
4170 1800 281
AgeI A'CCGG_T
Cuts at: 2541 2541
Size: 6251
AleI CACnn'nnGTG
Cuts at: 1237 1237
Size: 6251
AlwNI CAG_nnn'CTG
Cuts at: 164 202 259 381 795 940 964 1507 2239
Size: 38 57 122 414 145 24 543 732
Cuts at: 2239 2307 2431 2494 2734 3556 4994 164
Size: 68 124 63 240 822 1438 1421
Fragments arranged by size:
1438 1421 822 732 543 414 240 145
124 122 68 63 57 38 24
ApaI G_GGCC'C
Cuts at: 777 906 2709 3196 777
Size: 129 1803 487 3832
Fragments arranged by size:
3832 1803 487 129
ApaLI G'TGCA_C
Cuts at: 710 5089 710
Size: 4379 1872
ApoI r'AATT_y
Cuts at: 3255 5604 5860 3255
Size: 2349 256 3646
Fragments arranged by size:
3646 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6251
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6251
AvaI C'yCGr_G
Cuts at: 149 698 752 776 899 1716 2451 3089 3216
Size: 549 54 24 123 817 735 638 127
Cuts at: 3216 3271 149
Size: 55 3129
Fragments arranged by size:
3129 817 735 638 549 127 123 55
54 24
AvrII C'CTAG_G
Cuts at: 1068 1068
Size: 6251
BamHI G'GATC_C
Cuts at: 3276 3276
Size: 6251
BanI G'GyrC_C
Cuts at: 684 757 895 1317 1638 1667 1959 2220 3267
Size: 73 138 422 321 29 292 261 1047
Cuts at: 3267 3681 3929 3964 684
Size: 414 248 35 2971
Fragments arranged by size:
2971 1047 422 414 321 292 261 248
138 73 35 29
BanII G_rGCy'C
Cuts at: 350 499 705 777 906 974 982 1297 1351
Size: 149 206 72 129 68 8 315 54
Cuts at: 1351 2002 2252 2658 2709 2753 2896 3101 3128
Size: 651 250 406 51 44 143 205 27
Cuts at: 3128 3196 3223 3265 4295 350
Size: 68 27 42 1030 2306
Fragments arranged by size:
2306 1030 651 406 315 250 206 205
149 143 129 72 68 68 54 51
44 42 27 27 8
BbeI G_GCGC'C
Cuts at: 1671 3685 3933 1671
Size: 2014 248 3989
Fragments arranged by size:
3989 2014 248
BbsI GAAGACnn'nnnn_
Cuts at: 279 455 279
Size: 176 6075
BbvCI CC'TCA_GC
Cuts at: 1373 2243 2424 1373
Size: 870 181 5200
Fragments arranged by size:
5200 870 181
BciVI GTATCCnnnnn_n'
Cuts at: 430 1705 4149 5205 6251 430
Size: 1275 2444 1056 1046 430
Fragments arranged by size:
2444 1275 1056 1046 430
BclI T'GATC_A
Cuts at: 3771 3771
Size: 6251
BglI GCCn_nnn'nGGC
Cuts at: 755 1512 2381 2869 3218 755
Size: 757 869 488 349 3788
Fragments arranged by size:
3788 869 757 488 349
BglII A'GATC_T
Cuts at: 3766 5803 3766
Size: 2037 4214
BlpI GC'TnA_GC
Cuts at: 1497 2813 3095 3363 1497
Size: 1316 282 268 4385
Fragments arranged by size:
4385 1316 282 268
Bme1580I G_kGCm'C
Cuts at: 714 777 906 2709 3196 3876 3969 5093 714
Size: 63 129 1803 487 680 93 1124 1872
Fragments arranged by size:
1872 1803 1124 680 487 129 93 63
BmgBI CAC'GTC
Cuts at: 1059 1814 1856 1059
Size: 755 42 5454
Fragments arranged by size:
5454 755 42
BmrI ACTGGGnnnn_n'
Cuts at: 290 1349 2680 2722 2847 3074 3463 3544 3879
Size: 1059 1331 42 125 227 389 81 335
Cuts at: 3879 6023 290
Size: 2144 518
Fragments arranged by size:
2144 1331 1059 518 389 335 227 125
81 42
BmtI G_CTAG'C
Cuts at: 1307 1307
Size: 6251
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1235 1367 4618 1235
Size: 132 3251 2868
Fragments arranged by size:
3251 2868 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1146 1182 1230 1689 2456 2630 2932 817
Size: 329 36 48 459 767 174 302 4136
Fragments arranged by size:
4136 767 459 329 302 174 48 36
Bpu10I CC'TnA_GC
Cuts at: 976 1373 1532 1538 2243 2363 2424 976
Size: 397 159 6 705 120 61 4803
Fragments arranged by size:
4803 705 397 159 120 61 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2755 2790 3427 4306 5020 5318 2755
Size: 35 637 879 714 298 3688
Fragments arranged by size:
3688 879 714 637 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 2926 5955 2926
Size: 3029 3222
BsaAI yAC'GTr
Cuts at: 486 3604 486
Size: 3118 3133
BsaBI GATnn'nnATC
Cuts at: 6 3765 5802 6
Size: 3759 2037 455
Fragments arranged by size:
3759 2037 455
BsaHI Gr'CG_yC
Cuts at: 603 1668 2066 3682 3930 4632 6128 603
Size: 1065 398 1616 248 702 1496 726
Fragments arranged by size:
1616 1496 1065 726 702 398 248
BsaWI w'CCGG_w
Cuts at: 2541 2972 3440 3961 4639 5050 5197 5471 2541
Size: 431 468 521 678 411 147 274 3321
Fragments arranged by size:
3321 678 521 468 431 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 502 532 1177 1207 1728 1758 2091
Size: 30 30 30 645 30 521 30 333
Cuts at: 2091 2121 3096 3126 442
Size: 30 975 30 3567
Fragments arranged by size:
3567 975 645 521 333 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1143 1185 1251 1833 1860 1863 1937 2260 2311
Size: 42 66 582 27 3 74 323 51
Cuts at: 2311 2350 2412 2459 2733 3101 3214 1143
Size: 39 62 47 274 368 113 4180
Fragments arranged by size:
4180 582 368 323 274 113 74 66
62 51 47 42 39 27 3
BseYI C'CCAG_C
Cuts at: 139 395 630 1122 1140 1556 1623 1634 1787
Size: 256 235 492 18 416 67 11 153
Cuts at: 1787 1881 2007 2487 2664 3676 4434 5099 5507
Size: 94 126 480 177 1012 758 665 408
Cuts at: 5507 139
Size: 883
Fragments arranged by size:
1012 883 758 665 492 480 416 408
256 235 177 153 126 94 67 18
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 670 1093 310
Size: 360 423 5468
Fragments arranged by size:
5468 423 360
BsiEI CG_ry'CG
Cuts at: 104 181 193 747 3793 3839 5069 104
Size: 77 12 554 3046 46 1230 1286
Fragments arranged by size:
3046 1286 1230 554 77 46 12
BsiHKAI G_wGCw'C
Cuts at: 275 499 625 714 1297 2646 2814 3265 4043
Size: 224 126 89 583 1349 168 451 778
Cuts at: 4043 4233 5093 275
Size: 190 860 1433
Fragments arranged by size:
1433 1349 860 778 583 451 224 190
168 126 89
BsiWI C'GTAC_G
Cuts at: 723 723
Size: 6251
BsmI GAATG_Cn'
Cuts at: 1394 1394
Size: 6251
BsmBI CGTCTCn'nnnn_
Cuts at: 1164 1820 1845 1164
Size: 656 25 5570
Fragments arranged by size:
5570 656 25
Bsp1286I G_dGCh'C
Cuts at: 275 350 499 625 705 714 777 906 974
Size: 75 149 126 80 9 63 129 68
Cuts at: 974 982 1297 1351 2002 2252 2646 2658 2709
Size: 8 315 54 651 250 394 12 51
Cuts at: 2709 2753 2814 2896 3101 3128 3196 3223 3265
Size: 44 61 82 205 27 68 27 42
Cuts at: 3265 3876 3969 4043 4233 4295 5093 275
Size: 611 93 74 190 62 798 1433
Fragments arranged by size:
1433 798 651 611 394 315 250 205
190 149 129 126 93 82 80 75
74 68 68 63 62 61 54 51
44 42 27 27 12 9 8
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 6251
BspHI T'CATG_A
Cuts at: 4683 4683
Size: 6251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1750 3301 3515 3817 4198 1750
Size: 1551 214 302 381 3803
Fragments arranged by size:
3803 1551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 353 527 615 1136 1898 2916 4543 4597
Size: 303 174 88 521 762 1018 1627 54
Cuts at: 4597 6081 6246 50
Size: 1484 165 55
Fragments arranged by size:
1627 1484 1018 762 521 303 174 165
88 55 54
BsrDI GCAATG_nn'
Cuts at: 4163 4163
Size: 6251
BsrFI r'CCGG_y
Cuts at: 192 884 2541 4249 4430 192
Size: 692 1657 1708 181 2013
Fragments arranged by size:
2013 1708 1657 692 181
BssSI C'ACGA_G
Cuts at: 4522 5230 4522
Size: 708 5543
BstAPI GCAn_nnn'nTGC
Cuts at: 202 648 718 964 2227 2622 3628 202
Size: 446 70 246 1263 395 1006 2825
Fragments arranged by size:
2825 1263 1006 446 395 246 70
BstBI TT'CG_AA
Cuts at: 4612 4612
Size: 6251
BstXI CCAn_nnnn'nTGG
Cuts at: 1755 1755
Size: 6251
BstYI r'GATC_y
Cuts at: 1 2259 3276 3743 3766 4101 4347 4653 4665
Size: 2258 1017 467 23 335 246 306 12
Cuts at: 4665 4751 5803 1
Size: 86 1052 449
Fragments arranged by size:
2258 1052 1017 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 667 762 2294 2745 2831 3020 667
Size: 95 1532 451 86 189 3898
Fragments arranged by size:
3898 1532 451 189 95 86
BtgI C'CryG_G
Cuts at: 107 402 890 1675 2312 2636 2842 107
Size: 295 488 785 637 324 206 3516
Fragments arranged by size:
3516 785 637 488 324 295 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4201 4382 4545 4201
Size: 181 163 5907
Fragments arranged by size:
5907 181 163
BtsI GCAGTG_nn'
Cuts at: 269 941 1344 1367 1410 1508 2110 2695 3494
Size: 672 403 23 43 98 602 585 799
Cuts at: 3494 269
Size: 3026
Fragments arranged by size:
3026 799 672 602 585 403 98 43
23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1895 1930 2544 2579 1895
Size: 35 614 35 5567
Fragments arranged by size:
5567 614 35 35
DraI TTT'AAA
Cuts at: 3250 3250
Size: 6251
DrdI GACnn_nn'nnGTC
Cuts at: 1936 3957 5301 1936
Size: 2021 1344 2886
Fragments arranged by size:
2886 2021 1344
EaeI y'GGCC_r
Cuts at: 190 243 744 882 983 1115 1419 1567 2354
Size: 53 501 138 101 132 304 148 787
Cuts at: 2354 2519 2786 2870 2992 3242 3836 4010 4401
Size: 165 267 84 122 250 594 174 391
Cuts at: 4401 4428 5773 190
Size: 27 1345 668
Fragments arranged by size:
1345 787 668 594 501 391 304 267
250 174 165 148 138 132 122 101
84 53 27
EagI C'GGCC_G
Cuts at: 190 744 3836 190
Size: 554 3092 2605
Fragments arranged by size:
3092 2605 554
EarI CTCTTCn'nnn_
Cuts at: 314 1823 1851 4274 4484 314
Size: 1509 28 2423 210 2081
Fragments arranged by size:
2423 2081 1509 210 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 633 817 823 2468 2493 3178 4459 5201 5347
Size: 184 6 1645 25 685 1281 742 146
Cuts at: 5347 6061 633
Size: 714 823
Fragments arranged by size:
1645 1281 823 742 714 685 184 146
25 6
EcoICRI GAG'CTC
Cuts at: 497 1295 3263 497
Size: 798 1968 3485
Fragments arranged by size:
3485 1968 798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1146 1182 1230 1465 1689 2363 2456 2630
Size: 329 36 48 235 224 674 93 174
Cuts at: 2630 2932 4076 4508 4861 5523 817
Size: 302 1144 432 353 662 1545
Fragments arranged by size:
1545 1144 674 662 432 353 329 302
235 224 174 93 48 36
EcoO109I rG'GnC_Cy
Cuts at: 135 238 773 903 961 1752 1762 1832 2304
Size: 103 535 130 58 791 10 70 472
Cuts at: 2304 2374 2705 2706 3003 3390 135
Size: 70 331 1 297 387 2996
Fragments arranged by size:
2996 791 535 472 387 331 297 130
103 70 70 58 10 1
EcoRI G'AATT_C
Cuts at: 3255 3255
Size: 6251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4270 4270
Size: 6251
FspI TGC'GCA
Cuts at: 1087 4032 1087
Size: 2945 3306
HaeII r_GCGC'y
Cuts at: 796 821 846 1149 1671 3685 3933 5163 5934
Size: 25 25 303 522 2014 248 1230 771
Cuts at: 5934 796
Size: 1113
Fragments arranged by size:
2014 1230 1113 771 522 303 248 25
25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 502 534 2209 2241 4093 4123 4125
Size: 32 28 32 1675 32 1852 30 2
Cuts at: 4125 4155 442
Size: 30 2538
Fragments arranged by size:
2538 1852 1675 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 3290 3290
Size: 6251
KasI G'GCGC_C
Cuts at: 1667 3681 3929 1667
Size: 2014 248 3989
Fragments arranged by size:
3989 2014 248
KpnI G_GTAC'C
Cuts at: 899 1321 1642 3271 899
Size: 422 321 1629 3879
Fragments arranged by size:
3879 1629 422 321
MluI A'CGCG_T
Cuts at: 5684 5684
Size: 6251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 585 1253 2790 5010 5194 585
Size: 668 1537 2220 184 1642
Fragments arranged by size:
2220 1642 1537 668 184
MscI TGG'CCA
Cuts at: 245 2356 2521 4012 245
Size: 2111 165 1491 2484
Fragments arranged by size:
2484 2111 1491 165
MslI CAynn'nnrTG
Cuts at: 230 1237 4240 4367 230
Size: 1007 3003 127 2114
Fragments arranged by size:
3003 2114 1007 127
MspA1I CmG'CkG
Cuts at: 143 1076 1100 1491 1560 1634 2011 2910 2952
Size: 933 24 391 69 74 377 899 42
Cuts at: 2952 2991 3186 3362 3676 4036 4820 5065 143
Size: 39 195 176 314 360 784 245 1329
Fragments arranged by size:
1329 933 899 784 391 377 360 314
245 195 176 74 69 42 39 24
NaeI GCC'GGC
Cuts at: 194 4432 194
Size: 4238 2013
NarI GG'CG_CC
Cuts at: 1668 3682 3930 1668
Size: 2014 248 3989
Fragments arranged by size:
3989 2014 248
NcoI C'CATG_G
Cuts at: 107 402 2312 107
Size: 295 1910 4046
Fragments arranged by size:
4046 1910 295
NgoMIV G'CCGG_C
Cuts at: 192 4430 192
Size: 4238 2013
NheI G'CTAG_C
Cuts at: 1303 1303
Size: 6251
NspI r_CATG'y
Cuts at: 649 1036 2518 3304 3804 5407 649
Size: 387 1482 786 500 1603 1493
Fragments arranged by size:
1603 1493 1482 786 500 387
PasI CC'CwG_GG
Cuts at: 1329 1828 1923 2509 2573 1329
Size: 499 95 586 64 5007
Fragments arranged by size:
5007 586 499 95 64
PciI A'CATG_T
Cuts at: 5403 5403
Size: 6251
PflMI CCAn_nnn'nTGG
Cuts at: 1634 1634
Size: 6251
PfoI T'CCnGG_A
Cuts at: 430 2646 430
Size: 2216 4035
PmeI GTTT'AAAC
Cuts at: 3250 3250
Size: 6251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 4697
Size: 32 6219
PpuMI rG'GwC_Cy
Cuts at: 1762 2304 1762
Size: 542 5709
PshAI GACnn'nnGTC
Cuts at: 2069 2069
Size: 6251
PspOMI G'GGCC_C
Cuts at: 773 902 2705 3192 773
Size: 129 1803 487 3832
Fragments arranged by size:
3832 1803 487 129
PspXI vC'TCGA_Gb
Cuts at: 1716 3089 1716
Size: 1373 4878
PstI C_TGCA'G
Cuts at: 264 469 791 842 969 1081 1105 2117 2373
Size: 205 322 51 127 112 24 1012 256
Cuts at: 2373 2618 2782 2909 3185 3298 3983 264
Size: 245 164 127 276 113 685 2532
Fragments arranged by size:
2532 1012 685 322 276 256 245 205
164 127 127 113 112 51 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6251
PvuII CAG'CTG
Cuts at: 1560 1634 2011 2910 3186 3676 4036 1560
Size: 74 377 899 276 490 360 3775
Fragments arranged by size:
3775 899 490 377 360 276 74
RsrII CG'GwC_CG
Cuts at: 2763 2824 4446 2763
Size: 61 1622 4568
Fragments arranged by size:
4568 1622 61
SacI G_AGCT'C
Cuts at: 499 1297 3265 499
Size: 798 1968 3485
Fragments arranged by size:
3485 1968 798
SalI G'TCGA_C
Cuts at: 3288 3288
Size: 6251
SapI GCTCTTCn'nnn_
Cuts at: 314 4274 4484 314
Size: 3960 210 2081
Fragments arranged by size:
3960 2081 210
SbfI CC_TGCA'GG
Cuts at: 791 2373 3298 791
Size: 1582 925 3744
Fragments arranged by size:
3744 1582 925
ScaI AGT'ACT
Cuts at: 5768 5768
Size: 6251
SfcI C'TryA_G
Cuts at: 33 260 465 787 838 965 1049 1077 1101
Size: 227 205 322 51 127 84 28 24
Cuts at: 1101 2113 2369 2550 2614 2778 2905 3181 3294
Size: 1012 256 181 64 164 127 276 113
Cuts at: 3294 3979 4947 5138 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1012 968 685 322 276 256 227
205 191 181 164 127 127 113 84
64 51 28 24
SfoI GGC'GCC
Cuts at: 1669 3683 3931 1669
Size: 2014 248 3989
Fragments arranged by size:
3989 2014 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6251
SmaI CCC'GGG
Cuts at: 151 700 778 901 2453 3218 3273 151
Size: 549 78 123 1552 765 55 3129
Fragments arranged by size:
3129 1552 765 549 123 78 55
SmlI C'TyrA_G
Cuts at: 1716 2770 2805 3089 3406 4321 5035 5297 1716
Size: 1054 35 284 317 915 714 262 2670
Fragments arranged by size:
2670 1054 915 714 317 284 262 35
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6251
SphI G_CATG'C
Cuts at: 3304 3804 3304
Size: 500 5751
StuI AGG'CCT
Cuts at: 1378 2776 2801 1378
Size: 1398 25 4828
Fragments arranged by size:
4828 1398 25
StyI C'CwwG_G
Cuts at: 107 206 402 1068 1748 1955 2312 2888 3385
Size: 99 196 666 680 207 357 576 497
Cuts at: 3385 3739 107
Size: 354 2619
Fragments arranged by size:
2619 680 666 576 497 357 354 207
196 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 427 1202 1587 1632 1915 2205 2629 3151 3834
Size: 775 385 45 283 290 424 522 683
Cuts at: 3834 3967 4426 4635 427
Size: 133 459 209 2043
Fragments arranged by size:
2043 775 683 522 459 424 385 290
283 209 133 45
TatI w'GTAC_w
Cuts at: 5766 5766
Size: 6251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 663 695 1177 1207 1209 1239 1728 1760 5768
Size: 32 482 30 2 30 489 32 4008
Cuts at: 5768 5800 663
Size: 32 1114
Fragments arranged by size:
4008 1114 489 482 32 32 32 30
30 2
Tth111I GACn'n_nGTC
Cuts at: 283 856 4048 283
Size: 573 3192 2486
Fragments arranged by size:
3192 2486 573
XbaI T'CTAG_A
Cuts at: 3282 3282
Size: 6251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 1336 1631 2667 3746 483
Size: 853 295 1036 1079 2988
Fragments arranged by size:
2988 1079 1036 853 295
XhoI C'TCGA_G
Cuts at: 1716 3089 1716
Size: 1373 4878
XmaI C'CCGG_G
Cuts at: 149 698 776 899 2451 3216 3271 149
Size: 549 78 123 1552 765 55 3129
Fragments arranged by size:
3129 1552 765 549 123 78 55
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BcgI BfrBI BsrGI BssHII BstEII BstZ17I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PmlI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI