Restriction map for
pF1KA1525
(Circular) (Six-base) MAPSORT of: pF1KA1525.seq Check: 8716 from: 1 to: 6509
pF1KA1525 6509 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2103 2103
Size: 6509
AatII G_ACGT'C
Cuts at: 3100 3100
Size: 6509
AccI GT'mk_AC
Cuts at: 539 1105 2552 2786 3547 539
Size: 566 1447 234 761 3501
Fragments arranged by size:
3501 1447 761 566 234
Acc65I G'GTAC_C
Cuts at: 3525 3525
Size: 6509
AclI AA'CG_TT
Cuts at: 2256 6349 2256
Size: 4093 2416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 2009 2834 3368 4334 4766 5513 5781 854
Size: 1155 825 534 966 432 747 268 1582
Fragments arranged by size:
1582 1155 966 825 747 534 432 268
AflII C'TTAA_G
Cuts at: 1704 1704
Size: 6509
AflIII A'CryG_T
Cuts at: 836 3861 4965 5942 836
Size: 3025 1104 977 1403
Fragments arranged by size:
3025 1403 1104 977
AgeI A'CCGG_T
Cuts at: 3253 4897 3253
Size: 1644 4865
AhdI GACnn_n'nnGTC
Cuts at: 3390 3390
Size: 6509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1820 1820
Size: 6509
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1478 1510 1478
Size: 32 6477
AlwNI CAG_nnn'CTG
Cuts at: 877 2097 2209 2677 2812 2841 3814 5381 877
Size: 1220 112 468 135 29 973 1567 2005
Fragments arranged by size:
2005 1567 1220 973 468 135 112 29
ApaLI G'TGCA_C
Cuts at: 1360 5279 1360
Size: 3919 2590
ApoI r'AATT_y
Cuts at: 162 784 798 975 1249 1393 2042 2168 2239
Size: 622 14 177 274 144 649 126 71
Cuts at: 2239 3513 5862 6118 162
Size: 1274 2349 256 553
Fragments arranged by size:
2349 1274 649 622 553 274 256 177
144 126 71 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 6509
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
AvaI C'yCGr_G
Cuts at: 147 3529 147
Size: 3382 3127
BamHI G'GATC_C
Cuts at: 3534 3534
Size: 6509
BanI G'GyrC_C
Cuts at: 2149 3525 3939 4187 4222 2149
Size: 1376 414 248 35 4436
Fragments arranged by size:
4436 1376 414 248 35
BanII G_rGCy'C
Cuts at: 929 1634 3411 3523 4553 929
Size: 705 1777 112 1030 2885
Fragments arranged by size:
2885 1777 1030 705 112
BbeI G_GCGC'C
Cuts at: 2153 3943 4191 2153
Size: 1790 248 4471
Fragments arranged by size:
4471 1790 248
BbsI GAAGACnn'nnnn_
Cuts at: 3381 3381
Size: 6509
BciVI GTATCCnnnnn_n'
Cuts at: 986 4407 5168 6509 986
Size: 3421 761 1341 986
Fragments arranged by size:
3421 1341 986 761
BclI T'GATC_A
Cuts at: 1982 4029 1982
Size: 2047 4462
BfrBI ATG'CAT
Cuts at: 121 406 121
Size: 285 6224
BglI GCCn_nnn'nGGC
Cuts at: 466 586 940 466
Size: 120 354 6035
Fragments arranged by size:
6035 354 120
BglII A'GATC_T
Cuts at: 2346 4024 6061 2346
Size: 1678 2037 2794
Fragments arranged by size:
2794 2037 1678
BlpI GC'TnA_GC
Cuts at: 3621 3621
Size: 6509
Bme1580I G_kGCm'C
Cuts at: 1364 4134 4227 5283 1364
Size: 2770 93 1056 2590
Fragments arranged by size:
2770 2590 1056 93
BmgBI CAC'GTC
Cuts at: 1113 1113
Size: 6509
BmrI ACTGGGnnnn_n'
Cuts at: 2456 2542 2614 3721 3802 4137 6281 2456
Size: 86 72 1107 81 335 2144 2684
Fragments arranged by size:
2684 2144 1107 335 86 81 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 391 1314 4876 391
Size: 923 3562 2024
Fragments arranged by size:
3562 2024 923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2502 2502
Size: 6509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 1309 2740 3685 4564 5056 5354 726
Size: 583 1431 945 879 492 298 1881
Fragments arranged by size:
1881 1431 945 879 583 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6213 6213
Size: 6509
BsaAI yAC'GTr
Cuts at: 3862 3862
Size: 6509
BsaBI GATnn'nnATC
Cuts at: 6 4023 6060 6
Size: 4017 2037 455
Fragments arranged by size:
4017 2037 455
BsaHI Gr'CG_yC
Cuts at: 2150 3097 3940 4188 4890 6386 2150
Size: 947 843 248 702 1496 2273
Fragments arranged by size:
2273 1496 947 843 702 248
BsaWI w'CCGG_w
Cuts at: 2934 3253 3367 3698 4219 4897 5171 5318 5729
Size: 319 114 331 521 678 274 147 411
Cuts at: 5729 2934
Size: 3714
Fragments arranged by size:
3714 678 521 411 331 319 274 147
114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1146 1176 1146
Size: 30 6479
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 1730 2050 364
Size: 1366 320 4823
Fragments arranged by size:
4823 1366 320
BseYI C'CCAG_C
Cuts at: 2409 2834 3209 3934 4692 5269 5765 2409
Size: 425 375 725 758 577 496 3153
Fragments arranged by size:
3153 758 725 577 496 425 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 2137 2558 938
Size: 1199 421 4889
Fragments arranged by size:
4889 1199 421
BsiEI CG_ry'CG
Cuts at: 104 4051 4097 5305 104
Size: 3947 46 1208 1308
Fragments arranged by size:
3947 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 929 1364 1634 3411 3523 4301 4491 5283 929
Size: 435 270 1777 112 778 190 792 2155
Fragments arranged by size:
2155 1777 792 778 435 270 190 112
BsmBI CGTCTCn'nnnn_
Cuts at: 2062 3104 2062
Size: 1042 5467
Bsp1286I G_dGCh'C
Cuts at: 929 1364 1634 3411 3523 4134 4227 4301 4491
Size: 435 270 1777 112 611 93 74 190
Cuts at: 4491 4553 5283 929
Size: 62 730 2155
Fragments arranged by size:
2155 1777 730 611 435 270 190 112
93 74 62
BspEI T'CCGG_A
Cuts at: 2934 5729 2934
Size: 2795 3714
BspHI T'CATG_A
Cuts at: 1406 5685 1406
Size: 4279 2230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2103 3559 3773 4075 4456 2103
Size: 1456 214 302 381 4156
Fragments arranged by size:
4156 1456 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4801 4855 6339 6504 50
Size: 4751 54 1484 165 55
Fragments arranged by size:
4751 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 487 1283 1721 4421 487
Size: 796 438 2700 2575
Fragments arranged by size:
2700 2575 796 438
BsrFI r'CCGG_y
Cuts at: 3253 4507 4688 4897 3253
Size: 1254 181 209 4865
Fragments arranged by size:
4865 1254 209 181
BsrGI T'GTAC_A
Cuts at: 3038 3038
Size: 6509
BssSI C'ACGA_G
Cuts at: 4780 5138 4780
Size: 358 6151
BstAPI GCAn_nnn'nTGC
Cuts at: 2097 2209 3886 2097
Size: 112 1677 4720
Fragments arranged by size:
4720 1677 112
BstBI TT'CG_AA
Cuts at: 972 4870 972
Size: 3898 2611
BstEII G'GTnAC_C
Cuts at: 3461 3461
Size: 6509
BstYI r'GATC_y
Cuts at: 1 1640 1893 2346 3364 3534 4001 4024 4359
Size: 1639 253 453 1018 170 467 23 335
Cuts at: 4359 4605 5617 5703 5715 6061 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1639 1018 1012 467 453 449 346 335
253 246 170 86 23 12
BstZ17I GTA'TAC
Cuts at: 1106 1106
Size: 6509
BtgI C'CryG_G
Cuts at: 107 249 1217 3402 107
Size: 142 968 2185 3214
Fragments arranged by size:
3214 2185 968 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2712 4459 4640 4803 2712
Size: 1747 181 163 4418
Fragments arranged by size:
4418 1747 181 163
BtsI GCAGTG_nn'
Cuts at: 613 1574 2594 3752 613
Size: 961 1020 1158 3370
Fragments arranged by size:
3370 1158 1020 961
DraI TTT'AAA
Cuts at: 609 3508 609
Size: 2899 3610
DraIII CAC_nnn'GTG
Cuts at: 3064 3064
Size: 6509
DrdI GACnn_nn'nnGTC
Cuts at: 2462 4215 5073 2462
Size: 1753 858 3898
Fragments arranged by size:
3898 1753 858
EaeI y'GGCC_r
Cuts at: 252 4094 4268 4659 4686 6031 252
Size: 3842 174 391 27 1345 730
Fragments arranged by size:
3842 1345 730 391 174 27
EagI C'GGCC_G
Cuts at: 4094 4094
Size: 6509
EarI CTCTTCn'nnn_
Cuts at: 917 1622 1837 3353 4532 4742 917
Size: 705 215 1516 1179 210 2684
Fragments arranged by size:
2684 1516 1179 705 215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4717 5027 5173 6319 4717
Size: 310 146 1146 4907
Fragments arranged by size:
4907 1146 310 146
EcoICRI GAG'CTC
Cuts at: 927 1632 3409 3521 927
Size: 705 1777 112 3915
Fragments arranged by size:
3915 1777 705 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 2009 2502 2834 3368 4334 4766 5513 5781
Size: 1155 493 332 534 966 432 747 268
Cuts at: 5781 854
Size: 1582
Fragments arranged by size:
1582 1155 966 747 534 493 432 332
268
EcoNI CCTnn'n_nnAGG
Cuts at: 1387 3011 1387
Size: 1624 4885
EcoO109I rG'GnC_Cy
Cuts at: 191 1614 2123 2827 3648 191
Size: 1423 509 704 821 3052
Fragments arranged by size:
3052 1423 821 704 509
EcoRI G'AATT_C
Cuts at: 3513 3513
Size: 6509
EcoRV GAT'ATC
Cuts at: 762 1281 1494 762
Size: 519 213 5777
Fragments arranged by size:
5777 519 213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 2039 3495 4528 1513
Size: 526 1456 1033 3494
Fragments arranged by size:
3494 1456 1033 526
FspI TGC'GCA
Cuts at: 4290 4290
Size: 6509
HaeII r_GCGC'y
Cuts at: 2153 3943 4191 5213 6192 2153
Size: 1790 248 1022 979 2470
Fragments arranged by size:
2470 1790 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 1323 1355 1722 1754 2338 2370 3117
Size: 32 876 32 367 32 584 32 747
Cuts at: 3117 3149 3376 3408 4351 4381 4383 4413 415
Size: 32 227 32 943 30 2 30 2511
Fragments arranged by size:
2511 943 876 747 584 367 227 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3548 3548
Size: 6509
HindIII A'AGCT_T
Cuts at: 890 1370 890
Size: 480 6029
KasI G'GCGC_C
Cuts at: 2149 3939 4187 2149
Size: 1790 248 4471
Fragments arranged by size:
4471 1790 248
KpnI G_GTAC'C
Cuts at: 3529 3529
Size: 6509
MluI A'CGCG_T
Cuts at: 5942 5942
Size: 6509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 307 5180 5364 307
Size: 4873 184 1452
Fragments arranged by size:
4873 1452 184
MscI TGG'CCA
Cuts at: 254 4270 254
Size: 4016 2493
MslI CAynn'nnrTG
Cuts at: 254 1343 2731 3188 4498 4625 254
Size: 1089 1388 457 1310 127 2138
Fragments arranged by size:
2138 1388 1310 1089 457 127
MspA1I CmG'CkG
Cuts at: 670 3213 3620 3934 4294 5307 5552 670
Size: 2543 407 314 360 1013 245 1627
Fragments arranged by size:
2543 1627 1013 407 360 314 245
NaeI GCC'GGC
Cuts at: 4690 4690
Size: 6509
NarI GG'CG_CC
Cuts at: 2150 3940 4188 2150
Size: 1790 248 4471
Fragments arranged by size:
4471 1790 248
NcoI C'CATG_G
Cuts at: 107 249 1217 3402 107
Size: 142 968 2185 3214
Fragments arranged by size:
3214 2185 968 142
NdeI CA'TA_TG
Cuts at: 1344 1344
Size: 6509
NgoMIV G'CCGG_C
Cuts at: 4688 4688
Size: 6509
NsiI A_TGCA'T
Cuts at: 123 408 123
Size: 285 6224
NspI r_CATG'y
Cuts at: 840 1260 3341 3562 4062 4969 840
Size: 420 2081 221 500 907 2380
Fragments arranged by size:
2380 2081 907 500 420 221
PasI CC'CwG_GG
Cuts at: 3396 3396
Size: 6509
PciI A'CATG_T
Cuts at: 836 4965 836
Size: 4129 2380
PflMI CCAn_nnn'nTGG
Cuts at: 637 637
Size: 6509
PmeI GTTT'AAAC
Cuts at: 3508 3508
Size: 6509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5648 5680 5648
Size: 32 6477
PpuMI rG'GwC_Cy
Cuts at: 191 1614 191
Size: 1423 5086
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1667 1699 1667
Size: 32 6477
PstI C_TGCA'G
Cuts at: 2093 2217 2331 2601 3285 3556 4241 2093
Size: 124 114 270 684 271 685 4361
Fragments arranged by size:
4361 685 684 271 270 124 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6509
PvuII CAG'CTG
Cuts at: 670 3934 4294 670
Size: 3264 360 2885
Fragments arranged by size:
3264 2885 360
RsrII CG'GwC_CG
Cuts at: 4704 4704
Size: 6509
SacI G_AGCT'C
Cuts at: 929 1634 3411 3523 929
Size: 705 1777 112 3915
Fragments arranged by size:
3915 1777 705 112
SalI G'TCGA_C
Cuts at: 3546 3546
Size: 6509
SapI GCTCTTCn'nnn_
Cuts at: 917 1622 4532 4742 917
Size: 705 2910 210 2684
Fragments arranged by size:
2910 2684 705 210
SbfI CC_TGCA'GG
Cuts at: 3556 3556
Size: 6509
ScaI AGT'ACT
Cuts at: 1737 6026 1737
Size: 4289 2220
SexAI A'CCwGG_T
Cuts at: 3087 3087
Size: 6509
SfcI C'TryA_G
Cuts at: 33 1137 1474 2089 2213 2327 2597 2783 3281
Size: 1104 337 615 124 114 270 186 498
Cuts at: 3281 3552 4237 5230 5421 33
Size: 271 685 993 191 1121
Fragments arranged by size:
1121 1104 993 685 615 498 337 271
270 191 186 124 114
SfoI GGC'GCC
Cuts at: 2151 3941 4189 2151
Size: 1790 248 4471
Fragments arranged by size:
4471 1790 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
SmaI CCC'GGG
Cuts at: 3531 3531
Size: 6509
SmlI C'TyrA_G
Cuts at: 741 1324 1704 2755 3664 4579 5071 5333 741
Size: 583 380 1051 909 915 492 262 1917
Fragments arranged by size:
1917 1051 915 909 583 492 380 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6509
SphI G_CATG'C
Cuts at: 3562 4062 3562
Size: 500 6009
SspI AAT'ATT
Cuts at: 548 548
Size: 6509
StyI C'CwwG_G
Cuts at: 107 249 1217 3402 3643 3997 107
Size: 142 968 2185 241 354 2619
Fragments arranged by size:
2619 2185 968 354 241 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 371 1034 1347 2725 4092 4225 4684 4893
Size: 271 663 313 1378 1367 133 459 209
Cuts at: 4893 100
Size: 1716
Fragments arranged by size:
1716 1378 1367 663 459 313 271 209
133
TatI w'GTAC_w
Cuts at: 518 529 1735 2317 3038 6024 518
Size: 11 1206 582 721 2986 1003
Fragments arranged by size:
2986 1206 1003 721 582 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1146 1178 6026 6058 1146
Size: 32 4848 32 1597
Fragments arranged by size:
4848 1597 32 32
Tth111I GACn'n_nGTC
Cuts at: 2077 4306 2077
Size: 2229 4280
XbaI T'CTAG_A
Cuts at: 3540 3540
Size: 6509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4004 4004
Size: 6509
XmaI C'CCGG_G
Cuts at: 3529 3529
Size: 6509
ZraI GAC'GTC
Cuts at: 3098 3098
Size: 6509
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AleI ApaI AscI AvrII BaeI BbvCI BcgI
BmtI Bpu10I BsiWI BsmI BssHII BstXI Bsu36I ClaI
CspCI FseI FspAI HpaI MfeI NheI NotI NruI
PacI PfoI PmlI PshAI PsiI PspOMI PspXI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI