Restriction map for
pF1KA1533
(Circular) (Six-base) MAPSORT of: pF1KA1533.seq Check: 2432 from: 1 to: 5288
pF1KA1533 5288 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1019 2326 1019
Size: 1307 3981
Acc65I G'GTAC_C
Cuts at: 2304 2304
Size: 5288
AclI AA'CG_TT
Cuts at: 5128 5128
Size: 5288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 604 3113 3545 3898 4560 586
Size: 18 2509 432 353 662 1314
Fragments arranged by size:
2509 1314 662 432 353 18
AflIII A'CryG_T
Cuts at: 577 2640 4440 4721 577
Size: 2063 1800 281 1144
Fragments arranged by size:
2063 1800 1144 281
AhdI GACnn_n'nnGTC
Cuts at: 734 734
Size: 5288
AleI CACnn'nnGTG
Cuts at: 241 1336 241
Size: 1095 4193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 5288
AlwNI CAG_nnn'CTG
Cuts at: 198 1458 1840 2163 2593 4031 198
Size: 1260 382 323 430 1438 1455
Fragments arranged by size:
1455 1438 1260 430 382 323
ApaI G_GGCC'C
Cuts at: 672 1106 1120 1426 1826 1921 672
Size: 434 14 306 400 95 4039
Fragments arranged by size:
4039 434 400 306 95 14
ApaLI G'TGCA_C
Cuts at: 2143 4126 2143
Size: 1983 3305
ApoI r'AATT_y
Cuts at: 2292 4641 4897 2292
Size: 2349 256 2683
Fragments arranged by size:
2683 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
AvaI C'yCGr_G
Cuts at: 891 965 1074 1757 1853 1872 1921 2308 891
Size: 74 109 683 96 19 49 387 3871
Fragments arranged by size:
3871 683 387 109 96 74 49 19
AvrII C'CTAG_G
Cuts at: 309 309
Size: 5288
BamHI G'GATC_C
Cuts at: 2313 2313
Size: 5288
BanI G'GyrC_C
Cuts at: 235 282 752 2304 2718 2966 3001 235
Size: 47 470 1552 414 248 35 2522
Fragments arranged by size:
2522 1552 470 414 248 47 35
BanII G_rGCy'C
Cuts at: 373 672 749 1088 1106 1120 1351 1426 1712
Size: 299 77 339 18 14 231 75 286
Cuts at: 1712 1812 1826 1879 1921 2183 2302 3332 373
Size: 100 14 53 42 262 119 1030 2329
Fragments arranged by size:
2329 1030 339 299 286 262 231 119
100 77 75 53 42 18 14 14
BbeI G_GCGC'C
Cuts at: 2722 2970 2722
Size: 248 5040
BbsI GAAGACnn'nnnn_
Cuts at: 1239 1595 1688 1239
Size: 356 93 4839
Fragments arranged by size:
4839 356 93
BbvCI CC'TCA_GC
Cuts at: 1882 1882
Size: 5288
BciVI GTATCCnnnnn_n'
Cuts at: 3186 4242 5288 3186
Size: 1056 1046 3186
Fragments arranged by size:
3186 1056 1046
BclI T'GATC_A
Cuts at: 2808 2808
Size: 5288
BglI GCCn_nnn'nGGC
Cuts at: 1101 1101
Size: 5288
BglII A'GATC_T
Cuts at: 626 2803 4840 626
Size: 2177 2037 1074
Fragments arranged by size:
2177 2037 1074
BlpI GC'TnA_GC
Cuts at: 367 1262 1345 2400 367
Size: 895 83 1055 3255
Fragments arranged by size:
3255 1055 895 83
Bme1580I G_kGCm'C
Cuts at: 672 1106 1120 1426 1826 1854 1921 1944 2147
Size: 434 14 306 400 28 67 23 203
Cuts at: 2147 2913 3006 4130 672
Size: 766 93 1124 1830
Fragments arranged by size:
1830 1124 766 434 400 306 203 93
67 28 23 14
BmgBI CAC'GTC
Cuts at: 1338 1338
Size: 5288
BmrI ACTGGGnnnn_n'
Cuts at: 919 1130 1190 1429 1931 2500 2581 2916 5060
Size: 211 60 239 502 569 81 335 2144
Cuts at: 5060 919
Size: 1147
Fragments arranged by size:
2144 1147 569 502 335 239 211 81
60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1680 3655 1680
Size: 1975 3313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1026 1255 1288 1374 1720 1756 2227 466
Size: 560 229 33 86 346 36 471 3527
Fragments arranged by size:
3527 560 471 346 229 86 36 33
Bpu10I CC'TnA_GC
Cuts at: 221 874 1798 1882 221
Size: 653 924 84 3627
Fragments arranged by size:
3627 924 653 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 3343 4057 4355 2464
Size: 879 714 298 3397
Fragments arranged by size:
3397 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 538 4992 538
Size: 4454 834
BsaAI yAC'GTr
Cuts at: 2641 2641
Size: 5288
BsaBI GATnn'nnATC
Cuts at: 6 556 2802 4839 6
Size: 550 2246 2037 455
Fragments arranged by size:
2246 2037 550 455
BsaHI Gr'CG_yC
Cuts at: 1034 2719 2967 3669 5165 1034
Size: 1685 248 702 1496 1157
Fragments arranged by size:
1685 1496 1157 702 248
BsaWI w'CCGG_w
Cuts at: 179 401 2477 2998 3676 4087 4234 4508 179
Size: 222 2076 521 678 411 147 274 959
Fragments arranged by size:
2076 959 678 521 411 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 799 829 871 901 1024 1054 799
Size: 30 42 30 123 30 5033
Fragments arranged by size:
5033 123 42 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 255 679 879 1012 1024 1029 1156 1158 1222
Size: 424 200 133 12 5 127 2 64
Cuts at: 1222 255
Size: 4321
Fragments arranged by size:
4321 424 200 133 127 64 12 5
2
BseYI C'CCAG_C
Cuts at: 295 539 783 1091 1826 2255 2713 3471 4136
Size: 244 244 308 735 429 458 758 665
Cuts at: 4136 4544 295
Size: 408 1039
Fragments arranged by size:
1039 758 735 665 458 429 408 308
244 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 615 2200 615
Size: 1585 3703
BsiEI CG_ry'CG
Cuts at: 104 1227 2830 2876 4106 104
Size: 1123 1603 46 1230 1286
Fragments arranged by size:
1603 1286 1230 1123 46
BsiHKAI G_wGCw'C
Cuts at: 133 157 749 1081 1712 2147 2183 2302 3080
Size: 24 592 332 631 435 36 119 778
Cuts at: 3080 3270 4130 133
Size: 190 860 1291
Fragments arranged by size:
1291 860 778 631 592 435 332 190
119 36 24
BsmI GAATG_Cn'
Cuts at: 717 1862 717
Size: 1145 4143
Bsp1286I G_dGCh'C
Cuts at: 133 157 373 672 749 1081 1088 1106 1120
Size: 24 216 299 77 332 7 18 14
Cuts at: 1120 1351 1426 1712 1812 1826 1854 1879 1921
Size: 231 75 286 100 14 28 25 42
Cuts at: 1921 1944 2147 2183 2302 2913 3006 3080 3270
Size: 23 203 36 119 611 93 74 190
Cuts at: 3270 3332 4130 133
Size: 62 798 1291
Fragments arranged by size:
1291 798 611 332 299 286 231 216
203 190 119 100 93 77 75 74
62 42 36 28 25 24 23 18
14 14 7
BspEI T'CCGG_A
Cuts at: 179 401 179
Size: 222 5066
BspHI T'CATG_A
Cuts at: 3720 3720
Size: 5288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1005 2338 2552 2854 3235 1005
Size: 1333 214 302 381 3058
Fragments arranged by size:
3058 1333 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1266 1673 3580 3634 5118 5283 50
Size: 1216 407 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 1216 407 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3200 3200
Size: 5288
BsrFI r'CCGG_y
Cuts at: 209 1480 1858 3286 3467 209
Size: 1271 378 1428 181 2030
Fragments arranged by size:
2030 1428 1271 378 181
BssSI C'ACGA_G
Cuts at: 1665 3559 4267 1665
Size: 1894 708 2686
Fragments arranged by size:
2686 1894 708
BstAPI GCAn_nnn'nTGC
Cuts at: 198 759 2163 2665 198
Size: 561 1404 502 2821
Fragments arranged by size:
2821 1404 561 502
BstBI TT'CG_AA
Cuts at: 3649 3649
Size: 5288
BstXI CCAn_nnnn'nTGG
Cuts at: 855 1113 1944 855
Size: 258 831 4199
Fragments arranged by size:
4199 831 258
BstYI r'GATC_y
Cuts at: 1 473 512 626 1125 1610 1836 2105 2159
Size: 472 39 114 499 485 226 269 54
Cuts at: 2159 2313 2780 2803 3138 3384 3690 3702 3788
Size: 154 467 23 335 246 306 12 86
Cuts at: 3788 4840 1
Size: 1052 449
Fragments arranged by size:
1052 499 485 472 467 449 335 306
269 246 226 154 114 86 54 39
23 12
BtgI C'CryG_G
Cuts at: 107 239 939 987 1439 1630 2174 107
Size: 132 700 48 452 191 544 3221
Fragments arranged by size:
3221 700 544 452 191 132 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 158 1962 3238 3419 3582 158
Size: 1804 1276 181 163 1864
Fragments arranged by size:
1864 1804 1276 181 163
BtsI GCAGTG_nn'
Cuts at: 715 1327 1544 2531 715
Size: 612 217 987 3472
Fragments arranged by size:
3472 987 612 217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 908 943 908
Size: 35 5253
DraI TTT'AAA
Cuts at: 2287 2287
Size: 5288
DraIII CAC_nnn'GTG
Cuts at: 759 2096 2136 759
Size: 1337 40 3911
Fragments arranged by size:
3911 1337 40
DrdI GACnn_nn'nnGTC
Cuts at: 2994 4338 2994
Size: 1344 3944
EaeI y'GGCC_r
Cuts at: 147 599 942 1224 2063 2087 2873 3047 3438
Size: 452 343 282 839 24 786 174 391
Cuts at: 3438 3465 4810 147
Size: 27 1345 625
Fragments arranged by size:
1345 839 786 625 452 391 343 282
174 27 24
EagI C'GGCC_G
Cuts at: 1224 2873 1224
Size: 1649 3639
EarI CTCTTCn'nnn_
Cuts at: 1176 1288 2002 3311 3521 1176
Size: 112 714 1309 210 2943
Fragments arranged by size:
2943 1309 714 210 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 688 1800 3496 4238 4384 5098 688
Size: 1112 1696 742 146 714 878
Fragments arranged by size:
1696 1112 878 742 714 146
EcoICRI GAG'CTC
Cuts at: 747 1710 2181 2300 747
Size: 963 471 119 3735
Fragments arranged by size:
3735 963 471 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 586 604 1026 1255 1288 1374 1720 1756
Size: 120 18 422 229 33 86 346 36
Cuts at: 1756 2227 3113 3545 3898 4560 466
Size: 471 886 432 353 662 1194
Fragments arranged by size:
1194 886 662 471 432 422 353 346
229 120 86 36 33 18
EcoNI CCTnn'n_nnAGG
Cuts at: 481 2065 481
Size: 1584 3704
EcoO109I rG'GnC_Cy
Cuts at: 290 668 842 1117 1423 1823 1917 1918 2427
Size: 378 174 275 306 400 94 1 509
Cuts at: 2427 290
Size: 3151
Fragments arranged by size:
3151 509 400 378 306 275 174 94
1
EcoRI G'AATT_C
Cuts at: 2292 2292
Size: 5288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 3307 669
Size: 2638 2650
FspI TGC'GCA
Cuts at: 3069 3069
Size: 5288
HaeII r_GCGC'y
Cuts at: 1262 1383 1436 2116 2722 2970 4200 4971 1262
Size: 121 53 680 606 248 1230 771 1579
Fragments arranged by size:
1579 1230 771 680 606 248 121 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 250 270 282 720 752 799 831 851
Size: 12 20 12 438 32 47 32 20
Cuts at: 851 871 883 903 1024 1056 1714 1746 3130
Size: 20 12 20 121 32 658 32 1384
Cuts at: 3130 3160 3162 3192 238
Size: 30 2 30 2334
Fragments arranged by size:
2334 1384 658 438 121 47 32 32
32 32 30 30 20 20 20 20
12 12 12 2
HincII GTy'rAC
Cuts at: 2327 2327
Size: 5288
KasI G'GCGC_C
Cuts at: 2718 2966 2718
Size: 248 5040
KpnI G_GTAC'C
Cuts at: 2308 2308
Size: 5288
MluI A'CGCG_T
Cuts at: 4721 4721
Size: 5288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4047 4231 4047
Size: 184 5104
MscI TGG'CCA
Cuts at: 944 2065 3049 944
Size: 1121 984 3183
Fragments arranged by size:
3183 1121 984
MslI CAynn'nnrTG
Cuts at: 241 1336 3277 3404 241
Size: 1095 1941 127 2125
Fragments arranged by size:
2125 1941 1095 127
MspA1I CmG'CkG
Cuts at: 539 564 680 828 901 2399 2713 3073 3857
Size: 25 116 148 73 1498 314 360 784
Cuts at: 3857 4102 539
Size: 245 1725
Fragments arranged by size:
1725 1498 784 360 314 245 148 116
73 25
NaeI GCC'GGC
Cuts at: 211 1482 1860 3469 211
Size: 1271 378 1609 2030
Fragments arranged by size:
2030 1609 1271 378
NarI GG'CG_CC
Cuts at: 2719 2967 2719
Size: 248 5040
NcoI C'CATG_G
Cuts at: 107 239 987 107
Size: 132 748 4408
Fragments arranged by size:
4408 748 132
NdeI CA'TA_TG
Cuts at: 114 114
Size: 5288
NgoMIV G'CCGG_C
Cuts at: 209 1480 1858 3467 209
Size: 1271 378 1609 2030
Fragments arranged by size:
2030 1609 1271 378
NspI r_CATG'y
Cuts at: 581 2341 2841 4444 581
Size: 1760 500 1603 1425
Fragments arranged by size:
1760 1603 1425 500
PasI CC'CwG_GG
Cuts at: 1113 1113
Size: 5288
PciI A'CATG_T
Cuts at: 577 4440 577
Size: 3863 1425
PflMI CCAn_nnn'nTGG
Cuts at: 1698 2096 2136 1698
Size: 398 40 4850
Fragments arranged by size:
4850 398 40
PfoI T'CCnGG_A
Cuts at: 2183 2183
Size: 5288
PmeI GTTT'AAAC
Cuts at: 2287 2287
Size: 5288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2214 3734 3766 2182
Size: 32 1520 32 3704
Fragments arranged by size:
3704 1520 32 32
PpuMI rG'GwC_Cy
Cuts at: 290 842 290
Size: 552 4736
PsiI TTA'TAA
Cuts at: 380 380
Size: 5288
PspOMI G'GGCC_C
Cuts at: 668 1102 1116 1422 1822 1917 668
Size: 434 14 306 400 95 4039
Fragments arranged by size:
4039 434 400 306 95 14
PstI C_TGCA'G
Cuts at: 194 467 2120 2335 3020 194
Size: 273 1653 215 685 2462
Fragments arranged by size:
2462 1653 685 273 215
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5288
PvuII CAG'CTG
Cuts at: 564 828 901 2713 3073 564
Size: 264 73 1812 360 2779
Fragments arranged by size:
2779 1812 360 264 73
RsrII CG'GwC_CG
Cuts at: 3483 3483
Size: 5288
SacI G_AGCT'C
Cuts at: 749 1712 2183 2302 749
Size: 963 471 119 3735
Fragments arranged by size:
3735 963 471 119
SalI G'TCGA_C
Cuts at: 2325 2325
Size: 5288
SanDI GG'GwC_CC
Cuts at: 290 842 290
Size: 552 4736
SapI GCTCTTCn'nnn_
Cuts at: 1288 2002 3311 3521 1288
Size: 714 1309 210 3055
Fragments arranged by size:
3055 1309 714 210
SbfI CC_TGCA'GG
Cuts at: 2335 2335
Size: 5288
ScaI AGT'ACT
Cuts at: 4805 4805
Size: 5288
SexAI A'CCwGG_T
Cuts at: 755 755
Size: 5288
SfcI C'TryA_G
Cuts at: 33 190 463 522 1315 2116 2331 3016 3984
Size: 157 273 59 793 801 215 685 968
Cuts at: 3984 4175 33
Size: 191 1146
Fragments arranged by size:
1146 968 801 793 685 273 215 191
157 59
SfoI GGC'GCC
Cuts at: 2720 2968 2720
Size: 248 5040
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5288
SmaI CCC'GGG
Cuts at: 1759 1855 1923 2310 1759
Size: 96 68 387 4737
Fragments arranged by size:
4737 387 96 68
SmlI C'TyrA_G
Cuts at: 2443 3358 4072 4334 2443
Size: 915 714 262 3397
Fragments arranged by size:
3397 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5288
SphI G_CATG'C
Cuts at: 2341 2841 2341
Size: 500 4788
SrfI GCCC'GGGC
Cuts at: 1855 1855
Size: 5288
StyI C'CwwG_G
Cuts at: 107 239 309 848 987 1121 1715 2066 2220
Size: 132 70 539 139 134 594 351 154
Cuts at: 2220 2422 2776 107
Size: 202 354 2619
Fragments arranged by size:
2619 594 539 354 351 202 154 139
134 132 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 1105 1847 2269 2871 3004 3463 3672 316
Size: 789 742 422 602 133 459 209 1932
Fragments arranged by size:
1932 789 742 602 459 422 209 133
TatI w'GTAC_w
Cuts at: 4803 4803
Size: 5288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1354 1386 4805 4837 1354
Size: 32 3419 32 1805
Fragments arranged by size:
3419 1805 32 32
Tth111I GACn'n_nGTC
Cuts at: 811 3085 811
Size: 2274 3014
XbaI T'CTAG_A
Cuts at: 2319 2319
Size: 5288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2783 2783
Size: 5288
XmaI C'CCGG_G
Cuts at: 1757 1853 1921 2308 1757
Size: 96 68 387 4737
Fragments arranged by size:
4737 387 96 68
XmnI GAAnn'nnTTC
Cuts at: 409 649 2125 409
Size: 240 1476 3572
Fragments arranged by size:
3572 1476 240
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI AscI BaeI
BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PshAI
PspXI PsrI SacII SfiI SgrAI SnaBI SspI StuI
SwaI XhoI ZraI