Restriction map for
pF1KA1534
(Circular) (Six-base) MAPSORT of: pF1KA1534.seq Check: 4412 from: 1 to: 5546
pF1KA1534 5546 bp
With 182 enzymes: SgfI *
February 20, 2009 16:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 667 1317 667
Size: 650 4896
AccI GT'mk_AC
Cuts at: 176 2584 176
Size: 2408 3138
Acc65I G'GTAC_C
Cuts at: 1199 2562 1199
Size: 1363 4183
AclI AA'CG_TT
Cuts at: 5386 5386
Size: 5546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 555 1048 2077 3371 3803 4156 4818 526
Size: 29 493 1029 1294 432 353 662 1254
Fragments arranged by size:
1294 1254 1029 662 493 432 353 29
AfeI AGC'GCT
Cuts at: 465 465
Size: 5546
AflIII A'CryG_T
Cuts at: 1721 2898 4698 4979 1721
Size: 1177 1800 281 2288
Fragments arranged by size:
2288 1800 1177 281
AleI CACnn'nnGTG
Cuts at: 316 931 316
Size: 615 4931
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 766 798 1096 1128 766
Size: 32 298 32 5184
Fragments arranged by size:
5184 298 32 32
AlwNI CAG_nnn'CTG
Cuts at: 549 1797 2045 2355 2397 2851 4289 549
Size: 1248 248 310 42 454 1438 1806
Fragments arranged by size:
1806 1438 1248 454 310 248 42
ApaI G_GGCC'C
Cuts at: 293 514 909 1947 2153 293
Size: 221 395 1038 206 3686
Fragments arranged by size:
3686 1038 395 221 206
ApaLI G'TGCA_C
Cuts at: 4384 4384
Size: 5546
ApoI r'AATT_y
Cuts at: 1609 2550 4899 5155 1609
Size: 941 2349 256 2000
Fragments arranged by size:
2349 2000 941 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5546
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5546
AvaI C'yCGr_G
Cuts at: 1659 1770 2566 1659
Size: 111 796 4639
Fragments arranged by size:
4639 796 111
BamHI G'GATC_C
Cuts at: 2087 2239 2571 2087
Size: 152 332 5062
Fragments arranged by size:
5062 332 152
BanI G'GyrC_C
Cuts at: 131 318 655 1199 1812 2405 2448 2562 2976
Size: 187 337 544 613 593 43 114 414
Cuts at: 2976 3224 3259 131
Size: 248 35 2418
Fragments arranged by size:
2418 613 593 544 414 337 248 187
114 43 35
BanII G_rGCy'C
Cuts at: 227 260 293 514 535 909 1947 2153 2267
Size: 33 33 221 21 374 1038 206 114
Cuts at: 2267 2314 2428 2476 2560 3590 227
Size: 47 114 48 84 1030 2183
Fragments arranged by size:
2183 1038 1030 374 221 206 114 114
84 48 47 33 33 21
BbeI G_GCGC'C
Cuts at: 135 2980 3228 135
Size: 2845 248 2453
Fragments arranged by size:
2845 2453 248
BbsI GAAGACnn'nnnn_
Cuts at: 773 874 773
Size: 101 5445
BbvCI CC'TCA_GC
Cuts at: 208 735 1798 208
Size: 527 1063 3956
Fragments arranged by size:
3956 1063 527
BciVI GTATCCnnnnn_n'
Cuts at: 3444 4500 5546 3444
Size: 1056 1046 3444
Fragments arranged by size:
3444 1056 1046
BclI T'GATC_A
Cuts at: 3066 3066
Size: 5546
BfrBI ATG'CAT
Cuts at: 799 799
Size: 5546
BglI GCCn_nnn'nGGC
Cuts at: 509 1703 509
Size: 1194 4352
BglII A'GATC_T
Cuts at: 1655 3061 5098 1655
Size: 1406 2037 2103
Fragments arranged by size:
2103 2037 1406
BlpI GC'TnA_GC
Cuts at: 460 2422 2658 460
Size: 1962 236 3348
Fragments arranged by size:
3348 1962 236
Bme1580I G_kGCm'C
Cuts at: 293 514 658 909 1947 2153 3171 3264 4388
Size: 221 144 251 1038 206 1018 93 1124
Cuts at: 4388 293
Size: 1451
Fragments arranged by size:
1451 1124 1038 1018 251 221 206 144
93
BmgBI CAC'GTC
Cuts at: 1362 1673 1722 1863 1362
Size: 311 49 141 5045
Fragments arranged by size:
5045 311 141 49
BmrI ACTGGGnnnn_n'
Cuts at: 661 1719 2064 2758 2839 3174 5318 661
Size: 1058 345 694 81 335 2144 889
Fragments arranged by size:
2144 1058 889 694 345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1110 3913 1110
Size: 2803 2743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 808 1105 1128 1570 1840 1858 1942 637
Size: 171 297 23 442 270 18 84 4241
Fragments arranged by size:
4241 442 297 270 171 84 23 18
Bpu10I CC'TnA_GC
Cuts at: 208 735 1798 208
Size: 527 1063 3956
Fragments arranged by size:
3956 1063 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1603 2722 3601 4315 4613 388
Size: 1215 1119 879 714 298 1321
Fragments arranged by size:
1321 1215 1119 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 841 889 1258 5250 841
Size: 48 369 3992 1137
Fragments arranged by size:
3992 1137 369 48
BsaAI yAC'GTr
Cuts at: 2899 2899
Size: 5546
BsaBI GATnn'nnATC
Cuts at: 6 3060 5097 6
Size: 3054 2037 455
Fragments arranged by size:
3054 2037 455
BsaHI Gr'CG_yC
Cuts at: 132 611 973 2977 3225 3927 5423 132
Size: 479 362 2004 248 702 1496 255
Fragments arranged by size:
2004 1496 702 479 362 255 248
BsaWI w'CCGG_w
Cuts at: 1370 2735 3256 3934 4345 4492 4766 1370
Size: 1365 521 678 411 147 274 2150
Fragments arranged by size:
2150 1365 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 976 1006 1096 1126 1156 1670 1700 1825 1855
Size: 30 90 30 30 514 30 125 30
Cuts at: 1855 2191 2221 976
Size: 336 30 4301
Fragments arranged by size:
4301 514 336 125 90 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 196 847 964 1012 1174 1837 1939 2290
Size: 66 651 117 48 162 663 102 351
Cuts at: 2290 130
Size: 3386
Fragments arranged by size:
3386 663 651 351 162 117 102 66
48
BseYI C'CCAG_C
Cuts at: 478 553 954 963 1554 1601 2387 2971 3729
Size: 75 401 9 591 47 786 584 758
Cuts at: 3729 4394 4802 478
Size: 665 408 1222
Fragments arranged by size:
1222 786 758 665 591 584 408 401
75 47 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2341 2346 2383 2341
Size: 5 37 5504
Fragments arranged by size:
5504 37 5
BsiEI CG_ry'CG
Cuts at: 104 3088 3134 4364 104
Size: 2984 46 1230 1286
Fragments arranged by size:
2984 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 227 2267 2428 2560 3338 3528 4388 227
Size: 2040 161 132 778 190 860 1385
Fragments arranged by size:
2040 1385 860 778 190 161 132
BsmI GAATG_Cn'
Cuts at: 799 799
Size: 5546
BsmBI CGTCTCn'nnnn_
Cuts at: 668 668
Size: 5546
Bsp1286I G_dGCh'C
Cuts at: 227 260 293 514 535 658 909 1947 2153
Size: 33 33 221 21 123 251 1038 206
Cuts at: 2153 2267 2314 2428 2476 2560 3171 3264 3338
Size: 114 47 114 48 84 611 93 74
Cuts at: 3338 3528 3590 4388 227
Size: 190 62 798 1385
Fragments arranged by size:
1385 1038 798 611 251 221 206 190
123 114 114 93 84 74 62 48
47 33 33 21
BspHI T'CATG_A
Cuts at: 3978 3978
Size: 5546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 667 930 1145 1317 2596 2810 3112 3493 667
Size: 263 215 172 1279 214 302 381 2720
Fragments arranged by size:
2720 1279 381 302 263 215 214 172
BsrBI CCG'CTC
Cuts at: 50 1745 1775 1953 3838 3892 5376 5541 50
Size: 1695 30 178 1885 54 1484 165 55
Fragments arranged by size:
1885 1695 1484 178 165 55 54 30
BsrDI GCAATG_nn'
Cuts at: 3458 3458
Size: 5546
BsrFI r'CCGG_y
Cuts at: 324 425 3544 3725 324
Size: 101 3119 181 2145
Fragments arranged by size:
3119 2145 181 101
BssHII G'CGCG_C
Cuts at: 441 441
Size: 5546
BssSI C'ACGA_G
Cuts at: 2158 2359 3817 4525 2158
Size: 201 1458 708 3179
Fragments arranged by size:
3179 1458 708 201
BstAPI GCAn_nnn'nTGC
Cuts at: 1812 2355 2397 2923 1812
Size: 543 42 526 4435
Fragments arranged by size:
4435 543 526 42
BstBI TT'CG_AA
Cuts at: 3907 3907
Size: 5546
BstEII G'GTnAC_C
Cuts at: 384 1566 384
Size: 1182 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 933 2136 933
Size: 1203 4343
BstYI r'GATC_y
Cuts at: 1 1655 2087 2239 2571 3038 3061 3396 3642
Size: 1654 432 152 332 467 23 335 246
Cuts at: 3642 3948 3960 4046 5098 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1654 1052 467 449 432 335 332 306
246 152 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 830 830
Size: 5546
BtgI C'CryG_G
Cuts at: 2114 2225 2429 2114
Size: 111 204 5231
Fragments arranged by size:
5231 204 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 698 2049 3496 3677 3840 698
Size: 1351 1447 181 163 2404
Fragments arranged by size:
2404 1447 1351 181 163
BtsI GCAGTG_nn'
Cuts at: 1515 1758 2789 1515
Size: 243 1031 4272
Fragments arranged by size:
4272 1031 243
DraI TTT'AAA
Cuts at: 2545 2545
Size: 5546
DraIII CAC_nnn'GTG
Cuts at: 1812 1812
Size: 5546
DrdI GACnn_nn'nnGTC
Cuts at: 3252 4596 3252
Size: 1344 4202
EaeI y'GGCC_r
Cuts at: 1039 1704 2211 2414 3131 3305 3696 3723 5068
Size: 665 507 203 717 174 391 27 1345
Cuts at: 5068 1039
Size: 1517
Fragments arranged by size:
1517 1345 717 665 507 391 203 174
27
EagI C'GGCC_G
Cuts at: 3131 3131
Size: 5546
EarI CTCTTCn'nnn_
Cuts at: 3569 3779 3569
Size: 210 5336
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3754 4496 4642 5356 3754
Size: 742 146 714 3944
Fragments arranged by size:
3944 742 714 146
EcoICRI GAG'CTC
Cuts at: 225 2265 2426 2558 225
Size: 2040 161 132 3213
Fragments arranged by size:
3213 2040 161 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 555 637 808 1048 1105 1128 1570 1840
Size: 29 82 171 240 57 23 442 270
Cuts at: 1840 1858 1942 2077 3371 3803 4156 4818 526
Size: 18 84 135 1294 432 353 662 1254
Fragments arranged by size:
1294 1254 662 442 432 353 270 240
171 135 84 82 57 29 23 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2359 2359
Size: 5546
EcoO109I rG'GnC_Cy
Cuts at: 289 290 510 511 689 905 906 2049 2149
Size: 1 220 1 178 216 1 1143 100
Cuts at: 2149 2352 2685 289
Size: 203 333 3150
Fragments arranged by size:
3150 1143 333 220 216 203 178 100
1 1 1
EcoRI G'AATT_C
Cuts at: 1609 2550 1609
Size: 941 4605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1398 1608 1644 3565 1398
Size: 210 36 1921 3379
Fragments arranged by size:
3379 1921 210 36
FspI TGC'GCA
Cuts at: 3327 3327
Size: 5546
HaeII r_GCGC'y
Cuts at: 135 467 1444 2980 3228 4458 5229 135
Size: 332 977 1536 248 1230 771 452
Fragments arranged by size:
1536 1230 977 771 452 332 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 976 1008 1126 1158 1315 1347 1670
Size: 32 157 32 118 32 157 32 323
Cuts at: 1670 1702 1825 1857 2191 2223 3388 3418 3420
Size: 32 123 32 334 32 1165 30 2
Cuts at: 3420 3450 787
Size: 30 2883
Fragments arranged by size:
2883 1165 334 323 157 157 123 118
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 177 2585 177
Size: 2408 3138
KasI G'GCGC_C
Cuts at: 131 2976 3224 131
Size: 2845 248 2453
Fragments arranged by size:
2845 2453 248
KpnI G_GTAC'C
Cuts at: 1203 2566 1203
Size: 1363 4183
MluI A'CGCG_T
Cuts at: 4979 4979
Size: 5546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 1139 4305 4489 794
Size: 345 3166 184 1851
Fragments arranged by size:
3166 1851 345 184
MscI TGG'CCA
Cuts at: 1706 3307 1706
Size: 1601 3945
MslI CAynn'nnrTG
Cuts at: 316 931 3535 3662 316
Size: 615 2604 127 2200
Fragments arranged by size:
2604 2200 615 127
MspA1I CmG'CkG
Cuts at: 212 456 1274 1605 1818 1935 1941 1986 2181
Size: 244 818 331 213 117 6 45 195
Cuts at: 2181 2237 2517 2657 2971 3331 4115 4360 212
Size: 56 280 140 314 360 784 245 1398
Fragments arranged by size:
1398 818 784 360 331 314 280 245
244 213 195 140 117 56 45 6
NaeI GCC'GGC
Cuts at: 3727 3727
Size: 5546
NarI GG'CG_CC
Cuts at: 132 2977 3225 132
Size: 2845 248 2453
Fragments arranged by size:
2845 2453 248
NgoMIV G'CCGG_C
Cuts at: 3725 3725
Size: 5546
NsiI A_TGCA'T
Cuts at: 801 801
Size: 5546
NspI r_CATG'y
Cuts at: 2599 3099 4702 2599
Size: 500 1603 3443
Fragments arranged by size:
3443 1603 500
PasI CC'CwG_GG
Cuts at: 241 278 901 2045 2146 241
Size: 37 623 1144 101 3641
Fragments arranged by size:
3641 1144 623 101 37
PciI A'CATG_T
Cuts at: 4698 4698
Size: 5546
PfoI T'CCnGG_A
Cuts at: 944 944
Size: 5546
PmeI GTTT'AAAC
Cuts at: 2545 2545
Size: 5546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1096 1128 3992 4024 1096
Size: 32 2864 32 2618
Fragments arranged by size:
2864 2618 32 32
PpuMI rG'GwC_Cy
Cuts at: 689 2049 689
Size: 1360 4186
PspOMI G'GGCC_C
Cuts at: 289 510 905 1943 2149 289
Size: 221 395 1038 206 3686
Fragments arranged by size:
3686 1038 395 221 206
PstI C_TGCA'G
Cuts at: 2351 2471 2593 3278 2351
Size: 120 122 685 4619
Fragments arranged by size:
4619 685 122 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5546
PvuII CAG'CTG
Cuts at: 456 1605 1986 2517 2971 3331 456
Size: 1149 381 531 454 360 2671
Fragments arranged by size:
2671 1149 531 454 381 360
RsrII CG'GwC_CG
Cuts at: 3741 3741
Size: 5546
SacI G_AGCT'C
Cuts at: 227 2267 2428 2560 227
Size: 2040 161 132 3213
Fragments arranged by size:
3213 2040 161 132
SalI G'TCGA_C
Cuts at: 175 2583 175
Size: 2408 3138
SanDI GG'GwC_CC
Cuts at: 2049 2049
Size: 5546
SapI GCTCTTCn'nnn_
Cuts at: 3569 3779 3569
Size: 210 5336
SbfI CC_TGCA'GG
Cuts at: 2593 2593
Size: 5546
ScaI AGT'ACT
Cuts at: 5063 5063
Size: 5546
SfcI C'TryA_G
Cuts at: 33 1170 2347 2467 2589 3274 4242 4433 33
Size: 1137 1177 120 122 685 968 191 1146
Fragments arranged by size:
1177 1146 1137 968 685 191 122 120
SfoI GGC'GCC
Cuts at: 133 2978 3226 133
Size: 2845 248 2453
Fragments arranged by size:
2845 2453 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5546
SmaI CCC'GGG
Cuts at: 2568 2568
Size: 5546
SmlI C'TyrA_G
Cuts at: 403 1618 2701 3616 4330 4592 403
Size: 1215 1083 915 714 262 1357
Fragments arranged by size:
1357 1215 1083 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5546
SphI G_CATG'C
Cuts at: 2599 3099 2599
Size: 500 5046
StuI AGG'CCT
Cuts at: 121 2462 121
Size: 2341 3205
StyI C'CwwG_G
Cuts at: 1226 2680 3034 1226
Size: 1454 354 3738
Fragments arranged by size:
3738 1454 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 764 1546 2023 3129 3262 3721 3930 764
Size: 782 477 1106 133 459 209 2380
Fragments arranged by size:
2380 1106 782 477 459 209 133
TatI w'GTAC_w
Cuts at: 332 5061 332
Size: 4729 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5063 5095 5063
Size: 32 5514
Tth111I GACn'n_nGTC
Cuts at: 990 2293 3343 990
Size: 1303 1050 3193
Fragments arranged by size:
3193 1303 1050
XbaI T'CTAG_A
Cuts at: 2577 2577
Size: 5546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1080 1152 3041 1080
Size: 72 1889 3585
Fragments arranged by size:
3585 1889 72
XmaI C'CCGG_G
Cuts at: 2566 2566
Size: 5546
XmnI GAAnn'nnTTC
Cuts at: 1591 1591
Size: 5546
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AscI AvrII BaeI
BcgI BmtI BsiWI BspEI BsrGI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI