Restriction map for
pF1KA1555
(Circular) (Six-base) MAPSORT of: pF1KA1555.seq Check: 2833 from: 1 to: 10328
pF1KA1555 10328 bp
With 182 enzymes: SgfI *
November 19, 2008 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1476 2298 2613 6650 7008 1476
Size: 822 315 4037 358 4796
Fragments arranged by size:
4796 4037 822 358 315
AatII G_ACGT'C
Cuts at: 5444 5444
Size: 10328
AccI GT'mk_AC
Cuts at: 7366 7366
Size: 10328
Acc65I G'GTAC_C
Cuts at: 6277 7344 6277
Size: 1067 9261
AclI AA'CG_TT
Cuts at: 3101 10168 3101
Size: 7067 3261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 980 1190 1888 2098 3741 3835 5434 6184 6618
Size: 210 698 210 1643 94 1599 750 434
Cuts at: 6618 8153 8585 9332 9600 980
Size: 1535 432 747 268 1708
Fragments arranged by size:
1708 1643 1599 1535 750 747 698 434
432 268 210 210 94
AflII C'TTAA_G
Cuts at: 1209 1209
Size: 10328
AflIII A'CryG_T
Cuts at: 1841 3029 4395 6255 7680 8784 9761 1841
Size: 1188 1366 1860 1425 1104 977 2408
Fragments arranged by size:
2408 1860 1425 1366 1188 1104 977
AgeI A'CCGG_T
Cuts at: 8716 8716
Size: 10328
AleI CACnn'nnGTG
Cuts at: 752 4048 752
Size: 3296 7032
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1044 1076 1044
Size: 32 10296
AlwNI CAG_nnn'CTG
Cuts at: 958 1197 2161 3277 4162 5629 5891 7028 7633
Size: 239 964 1116 885 1467 262 1137 605
Cuts at: 7633 9200 958
Size: 1567 2086
Fragments arranged by size:
2086 1567 1467 1137 1116 964 885 605
262 239
ApaI G_GGCC'C
Cuts at: 292 3418 3446 4241 5806 6175 6755 6810 292
Size: 3126 28 795 1565 369 580 55 3810
Fragments arranged by size:
3810 3126 1565 795 580 369 55 28
ApaLI G'TGCA_C
Cuts at: 7112 9098 7112
Size: 1986 8342
ApoI r'AATT_y
Cuts at: 2495 3589 4640 5387 6889 7332 9681 9937 2495
Size: 1094 1051 747 1502 443 2349 256 2886
Fragments arranged by size:
2886 2349 1502 1094 1051 747 443 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10328
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10328
AvaI C'yCGr_G
Cuts at: 285 568 2326 2542 2901 3062 3402 6107 6248
Size: 283 1758 216 359 161 340 2705 141
Cuts at: 6248 6271 6809 6957 7126 7348 285
Size: 23 538 148 169 222 3265
Fragments arranged by size:
3265 2705 1758 538 359 340 283 222
216 169 161 148 141 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4873 4906 4873
Size: 33 10295
BamHI G'GATC_C
Cuts at: 111 908 6701 7353 111
Size: 797 5793 652 3086
Fragments arranged by size:
5793 3086 797 652
BanI G'GyrC_C
Cuts at: 133 1461 2140 3022 4803 5748 6277 6996 7344
Size: 1328 679 882 1781 945 529 719 348
Cuts at: 7344 7758 8006 8041 133
Size: 414 248 35 2420
Fragments arranged by size:
2420 1781 1328 945 882 719 679 529
414 348 248 35
BanII G_rGCy'C
Cuts at: 253 266 292 521 935 1001 1255 1576 1631
Size: 13 26 229 414 66 254 321 55
Cuts at: 1631 3097 3418 3446 4241 4816 5806 5953 6175
Size: 1466 321 28 795 575 990 147 222
Cuts at: 6175 6732 6755 6810 6853 7010 7093 7277 7342
Size: 557 23 55 43 157 83 184 65
Cuts at: 7342 8372 253
Size: 1030 2209
Fragments arranged by size:
2209 1466 1030 990 795 575 557 414
321 321 254 229 222 184 157 147
83 66 65 55 55 43 28 26
23 13
BbeI G_GCGC'C
Cuts at: 5752 7762 8010 5752
Size: 2010 248 8070
Fragments arranged by size:
8070 2010 248
BbsI GAAGACnn'nnnn_
Cuts at: 588 1121 1178 1451 2576 2752 3309 3803 5696
Size: 533 57 273 1125 176 557 494 1893
Cuts at: 5696 6012 588
Size: 316 4904
Fragments arranged by size:
4904 1893 1125 557 533 494 316 273
176 57
BbvCI CC'TCA_GC
Cuts at: 146 3801 3996 5807 6123 6399 6478 146
Size: 3655 195 1811 316 276 79 3996
Fragments arranged by size:
3996 3655 1811 316 276 195 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 404 438 4800 4834 7117 7151 404
Size: 34 4362 34 2283 34 3581
Fragments arranged by size:
4362 3581 2283 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8226 8987 10328 8226
Size: 761 1341 8226
Fragments arranged by size:
8226 1341 761
BclI T'GATC_A
Cuts at: 7848 7848
Size: 10328
BfrBI ATG'CAT
Cuts at: 2205 2205
Size: 10328
BglI GCCn_nnn'nGGC
Cuts at: 782 2703 3193 3910 6140 6814 782
Size: 1921 490 717 2230 674 4296
Fragments arranged by size:
4296 2230 1921 717 674 490
BglII A'GATC_T
Cuts at: 4127 5208 7843 9880 4127
Size: 1081 2635 2037 4575
Fragments arranged by size:
4575 2635 2037 1081
BlpI GC'TnA_GC
Cuts at: 1675 2943 4155 5870 5973 7440 1675
Size: 1268 1212 1715 103 1467 4563
Fragments arranged by size:
4563 1715 1467 1268 1212 103
Bme1580I G_kGCm'C
Cuts at: 136 292 2402 3025 3418 3446 3455 4241 5806
Size: 156 2110 623 393 28 9 786 1565
Cuts at: 5806 6175 6675 6755 6810 6999 7116 7284 7953
Size: 369 500 80 55 189 117 168 669
Cuts at: 7953 8046 9102 136
Size: 93 1056 1362
Fragments arranged by size:
2110 1565 1362 1056 786 669 623 500
393 369 189 168 156 117 93 80
55 28 9
BmrI ACTGGGnnnn_n'
Cuts at: 1083 1098 1688 2224 2580 2981 3184 3834 4134
Size: 15 590 536 356 401 203 650 300
Cuts at: 4134 7540 7621 7956 10100 1083
Size: 3406 81 335 2144 1311
Fragments arranged by size:
3406 2144 1311 650 590 536 401 356
335 300 203 81 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 6341 8695 3284
Size: 3057 2354 4917
Fragments arranged by size:
4917 3057 2354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 602 712 916 1014 1666 2138 2380 3167
Size: 133 110 204 98 652 472 242 787
Cuts at: 3167 3254 3569 3670 4381 4824 5575 5677 6311
Size: 87 315 101 711 443 751 102 634
Cuts at: 6311 6855 6931 7107 469
Size: 544 76 176 3690
Fragments arranged by size:
3690 787 751 711 652 634 544 472
443 315 242 204 176 133 110 102
101 98 87 76
Bpu10I CC'TnA_GC
Cuts at: 146 437 1156 1825 3801 3996 5807 6123 6399
Size: 291 719 669 1976 195 1811 316 276
Cuts at: 6399 6478 6878 146
Size: 79 400 3596
Fragments arranged by size:
3596 1976 1811 719 669 400 316 291
276 195 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4374 4513 6000 7504 8383 8875 9173 4374
Size: 139 1487 1504 879 492 298 5529
Fragments arranged by size:
5529 1504 1487 879 492 298 139
BsaI GGTCTCn'nnnn_
Cuts at: 477 624 5865 6194 6329 6487 6888 10032 477
Size: 147 5241 329 135 158 401 3144 773
Fragments arranged by size:
5241 3144 773 401 329 158 147 135
BsaAI yAC'GTr
Cuts at: 1842 6150 7681 1842
Size: 4308 1531 4489
Fragments arranged by size:
4489 4308 1531
BsaBI GATnn'nnATC
Cuts at: 6 5299 6691 7842 9879 6
Size: 5293 1392 1151 2037 455
Fragments arranged by size:
5293 2037 1392 1151 455
BsaHI Gr'CG_yC
Cuts at: 5441 5749 7068 7759 8007 8709 10205 5441
Size: 308 1319 691 248 702 1496 5564
Fragments arranged by size:
5564 1496 1319 702 691 308 248
BsaWI w'CCGG_w
Cuts at: 2452 7517 8038 8716 8990 9137 9548 2452
Size: 5065 521 678 274 147 411 3232
Fragments arranged by size:
5065 3232 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1044 1074 1618 1648 1933 1963 2503 2533 3704
Size: 30 544 30 285 30 540 30 1171
Cuts at: 3704 3734 3781 3811 3839 3869 3996 4026 4775
Size: 30 47 30 28 30 127 30 749
Cuts at: 4775 4805 5629 5659 1044
Size: 30 824 30 5713
Fragments arranged by size:
5713 1171 824 749 544 540 285 127
47 30 30 30 30 30 30 30
30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 943 979 1933 2265 2490 2707 2767 3240 3789
Size: 36 954 332 225 217 60 473 549
Cuts at: 3789 3984 4004 4477 4480 5253 5269 5392 5572
Size: 195 20 473 3 773 16 123 180
Cuts at: 5572 5650 5722 5725 5778 6200 6466 6555 6637
Size: 78 72 3 53 422 266 89 82
Cuts at: 6637 6891 943
Size: 254 4380
Fragments arranged by size:
4380 954 773 549 473 473 422 332
266 254 225 217 195 180 123 89
82 78 72 60 53 36 20 16
3 3
BseYI C'CCAG_C
Cuts at: 718 1418 1453 2768 2789 2796 3043 3725 3741
Size: 700 35 1315 21 7 247 682 16
Cuts at: 3741 3937 3984 4439 4712 4894 5404 5931 6089
Size: 196 47 455 273 182 510 527 158
Cuts at: 6089 6382 6656 7200 7753 8511 9088 9584 718
Size: 293 274 544 553 758 577 496 1462
Fragments arranged by size:
1462 1315 758 700 682 577 553 544
527 510 496 455 293 274 273 247
196 182 158 47 35 21 16 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1734 3583 3685 4238 6130 6634 6733 7014
Size: 409 1849 102 553 1892 504 99 281
Cuts at: 7014 1325
Size: 4639
Fragments arranged by size:
4639 1892 1849 553 504 409 281 102
99
BsiEI CG_ry'CG
Cuts at: 104 2707 5358 7057 7870 7916 9124 104
Size: 2603 2651 1699 813 46 1208 1308
Fragments arranged by size:
2651 2603 1699 1308 1208 813 46
BsiHKAI G_wGCw'C
Cuts at: 266 728 1001 1151 1499 1576 2058 4816 5642
Size: 462 273 150 348 77 482 2758 826
Cuts at: 5642 6255 6263 6853 7116 7342 8120 8310 9102
Size: 613 8 590 263 226 778 190 792
Cuts at: 9102 266
Size: 1492
Fragments arranged by size:
2758 1492 826 792 778 613 590 482
462 348 273 263 226 190 150 77
8
BsmI GAATG_Cn'
Cuts at: 415 415
Size: 10328
Bsp1286I G_dGCh'C
Cuts at: 136 253 266 292 521 728 935 1001 1151
Size: 117 13 26 229 207 207 66 150
Cuts at: 1151 1255 1499 1576 1631 2058 2402 3025 3097
Size: 104 244 77 55 427 344 623 72
Cuts at: 3097 3418 3446 3455 4241 4816 5642 5806 5953
Size: 321 28 9 786 575 826 164 147
Cuts at: 5953 6175 6255 6263 6675 6732 6755 6810 6853
Size: 222 80 8 412 57 23 55 43
Cuts at: 6853 6999 7010 7093 7116 7277 7284 7342 7953
Size: 146 11 83 23 161 7 58 611
Cuts at: 7953 8046 8120 8310 8372 9102 136
Size: 93 74 190 62 730 1362
Fragments arranged by size:
1362 826 786 730 623 611 575 427
412 344 321 244 229 222 207 207
190 164 161 150 147 146 117 104
93 83 80 77 74 72 66 62
58 57 55 55 43 28 26 23
23 13 11 9 8 7
BspEI T'CCGG_A
Cuts at: 9548 9548
Size: 10328
BspHI T'CATG_A
Cuts at: 9504 9504
Size: 10328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1305 1476 2298 2613 3530 6505 6650 7008 7378
Size: 171 822 315 917 2975 145 358 370
Cuts at: 7378 7592 7894 8275 1305
Size: 214 302 381 3358
Fragments arranged by size:
3358 2975 917 822 381 370 358 315
302 214 171 145
BsrBI CCG'CTC
Cuts at: 50 906 2942 8620 8674 10158 10323 50
Size: 856 2036 5678 54 1484 165 55
Fragments arranged by size:
5678 2036 1484 856 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2922 4112 8240 2922
Size: 1190 4128 5010
Fragments arranged by size:
5010 4128 1190
BsrFI r'CCGG_y
Cuts at: 701 1878 2702 5286 5937 6946 7116 8326 8507
Size: 1177 824 2584 651 1009 170 1210 181
Cuts at: 8507 8716 701
Size: 209 2313
Fragments arranged by size:
2584 2313 1210 1177 1009 824 651 209
181 170
BsrGI T'GTAC_A
Cuts at: 4109 5009 4109
Size: 900 9428
BssSI C'ACGA_G
Cuts at: 3559 5846 8599 8957 3559
Size: 2287 2753 358 4930
Fragments arranged by size:
4930 2753 2287 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3561 5534 7705 3561
Size: 1973 2171 6184
Fragments arranged by size:
6184 2171 1973
BstBI TT'CG_AA
Cuts at: 5306 8689 5306
Size: 3383 6945
BstEII G'GTnAC_C
Cuts at: 985 4134 985
Size: 3149 7179
BstXI CCAn_nnnn'nTGG
Cuts at: 3018 4720 6371 3018
Size: 1702 1651 6975
Fragments arranged by size:
6975 1702 1651
BstYI r'GATC_y
Cuts at: 1 111 908 2678 4127 4620 5208 6701 7353
Size: 110 797 1770 1449 493 588 1493 652
Cuts at: 7353 7820 7843 8178 8424 9436 9522 9534 9880
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9880 1
Size: 449
Fragments arranged by size:
1770 1493 1449 1012 797 652 588 493
467 449 346 335 246 110 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 861 2732 5059 5117 7260 7298 861
Size: 1871 2327 58 2143 38 3891
Fragments arranged by size:
3891 2327 2143 1871 58 38
BtgI C'CryG_G
Cuts at: 107 1529 2188 2417 2967 4046 4430 4671 5096
Size: 1422 659 229 550 1079 384 241 425
Cuts at: 5096 5352 6010 6349 6436 6508 6597 6984 107
Size: 256 658 339 87 72 89 387 3451
Fragments arranged by size:
3451 1422 1079 659 658 550 425 387
384 339 256 241 229 89 87 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6108 8278 8459 8622 6108
Size: 2170 181 163 7814
Fragments arranged by size:
7814 2170 181 163
BtsI GCAGTG_nn'
Cuts at: 1669 3466 3960 4518 5773 7571 1669
Size: 1797 494 558 1255 1798 4426
Fragments arranged by size:
4426 1798 1797 1255 558 494
ClaI AT'CG_AT
Cuts at: 1232 1232
Size: 10328
DraI TTT'AAA
Cuts at: 4934 5481 7327 4934
Size: 547 1846 7935
Fragments arranged by size:
7935 1846 547
DraIII CAC_nnn'GTG
Cuts at: 3394 3394
Size: 10328
DrdI GACnn_nn'nnGTC
Cuts at: 8034 8892 8034
Size: 858 9470
EaeI y'GGCC_r
Cuts at: 2704 3380 5099 5355 6600 6721 7054 7118 7913
Size: 676 1719 256 1245 121 333 64 795
Cuts at: 7913 8087 8478 8505 9850 2704
Size: 174 391 27 1345 3182
Fragments arranged by size:
3182 1719 1345 1245 795 676 391 333
256 174 121 64 27
EagI C'GGCC_G
Cuts at: 2704 5355 7054 7913 2704
Size: 2651 1699 859 5119
Fragments arranged by size:
5119 2651 1699 859
EarI CTCTTCn'nnn_
Cuts at: 632 914 956 2242 2257 2276 2818 4256 4537
Size: 282 42 1286 15 19 542 1438 281
Cuts at: 4537 4731 5228 5324 6228 6451 8351 8561 632
Size: 194 497 96 904 223 1900 210 2399
Fragments arranged by size:
2399 1900 1438 1286 904 542 497 282
281 223 210 194 96 42 19 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1584 2641 6570 6965 8536 8846 8992 10138 1584
Size: 1057 3929 395 1571 310 146 1146 1774
Fragments arranged by size:
3929 1774 1571 1146 1057 395 310 146
EcoICRI GAG'CTC
Cuts at: 264 999 1574 4814 6851 7340 264
Size: 735 575 3240 2037 489 3252
Fragments arranged by size:
3252 3240 2037 735 575 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 602 712 916 980 1014 1190 1666 1888
Size: 133 110 204 64 34 176 476 222
Cuts at: 1888 2098 2138 2380 3167 3254 3569 3670 3741
Size: 210 40 242 787 87 315 101 71
Cuts at: 3741 3835 4381 4824 5434 5575 5677 6184 6311
Size: 94 546 443 610 141 102 507 127
Cuts at: 6311 6618 6855 6931 7107 8153 8585 9332 9600
Size: 307 237 76 176 1046 432 747 268
Cuts at: 9600 469
Size: 1197
Fragments arranged by size:
1197 1046 787 747 610 546 507 476
443 432 315 307 268 242 237 222
210 204 176 176 141 133 127 110
102 101 94 87 76 71 64 40
34
EcoNI CCTnn'n_nnAGG
Cuts at: 1898 5120 5596 7180 1898
Size: 3222 476 1584 5046
Fragments arranged by size:
5046 3222 1584 476
EcoO109I rG'GnC_Cy
Cuts at: 289 356 573 761 2413 2982 3172 3414 3431
Size: 67 217 188 1652 569 190 242 17
Cuts at: 3431 3442 4976 5803 6172 6751 6806 7467 289
Size: 11 1534 827 369 579 55 661 3150
Fragments arranged by size:
3150 1652 1534 827 661 579 569 369
242 217 190 188 67 55 17 11
EcoRI G'AATT_C
Cuts at: 3589 5387 7332 3589
Size: 1798 1945 6585
Fragments arranged by size:
6585 1945 1798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4292 6393 8347 4292
Size: 2101 1954 6273
Fragments arranged by size:
6273 2101 1954
FspI TGC'GCA
Cuts at: 8109 8109
Size: 10328
HaeII r_GCGC'y
Cuts at: 1875 5752 5877 6361 7762 8010 9032 10011 1875
Size: 3877 125 484 1401 248 1022 979 2192
Fragments arranged by size:
3877 2192 1401 1022 979 484 248 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1650 1933 1965 2176 2208 4780 4812 4960
Size: 32 283 32 211 32 2572 32 148
Cuts at: 4960 4992 5653 5659 5685 5691 5713 5745 5956
Size: 32 661 6 26 6 22 32 211
Cuts at: 5956 5988 6838 6870 8170 8200 8202 8232 1618
Size: 32 850 32 1300 30 2 30 3714
Fragments arranged by size:
3714 2572 1300 850 661 283 211 211
148 32 32 32 32 32 32 32
32 30 30 26 22 6 6 2
HincII GTy'rAC
Cuts at: 474 3035 4745 7367 474
Size: 2561 1710 2622 3435
Fragments arranged by size:
3435 2622 2561 1710
HindIII A'AGCT_T
Cuts at: 4694 4694
Size: 10328
KasI G'GCGC_C
Cuts at: 5748 7758 8006 5748
Size: 2010 248 8070
Fragments arranged by size:
8070 2010 248
KpnI G_GTAC'C
Cuts at: 6281 7348 6281
Size: 1067 9261
MluI A'CGCG_T
Cuts at: 9761 9761
Size: 10328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3263 8999 9183 3263
Size: 5736 184 4408
Fragments arranged by size:
5736 4408 184
MscI TGG'CCA
Cuts at: 5101 8089 5101
Size: 2988 7340
MslI CAynn'nnrTG
Cuts at: 752 3028 3056 4048 8317 8444 752
Size: 2276 28 992 4269 127 2636
Fragments arranged by size:
4269 2636 2276 992 127 28
MspA1I CmG'CkG
Cuts at: 227 235 1034 1389 2800 4411 5354 5891 6204
Size: 8 799 355 1411 1611 943 537 313
Cuts at: 6204 6403 6833 7149 7439 7753 8113 9126 9371
Size: 199 430 316 290 314 360 1013 245
Cuts at: 9371 227
Size: 1184
Fragments arranged by size:
1611 1411 1184 1013 943 799 537 430
360 355 316 314 313 290 245 199
8
NaeI GCC'GGC
Cuts at: 703 1880 5288 8509 703
Size: 1177 3408 3221 2522
Fragments arranged by size:
3408 3221 2522 1177
NarI GG'CG_CC
Cuts at: 5749 7759 8007 5749
Size: 2010 248 8070
Fragments arranged by size:
8070 2010 248
NcoI C'CATG_G
Cuts at: 107 2967 4046 4430 4671 5096 6984 107
Size: 2860 1079 384 241 425 1888 3451
Fragments arranged by size:
3451 2860 1888 1079 425 384 241
NdeI CA'TA_TG
Cuts at: 2047 2047
Size: 10328
NgoMIV G'CCGG_C
Cuts at: 701 1878 5286 8507 701
Size: 1177 3408 3221 2522
Fragments arranged by size:
3408 3221 2522 1177
NsiI A_TGCA'T
Cuts at: 2207 2207
Size: 10328
NspI r_CATG'y
Cuts at: 3006 3033 3061 4966 5412 5773 5916 6259 7381
Size: 27 28 1905 446 361 143 343 1122
Cuts at: 7381 7881 8788 3006
Size: 500 907 4546
Fragments arranged by size:
4546 1905 1122 907 500 446 361 343
143 28 27
PasI CC'CwG_GG
Cuts at: 514 913 3018 3418 6228 7062 7277 514
Size: 399 2105 400 2810 834 215 3565
Fragments arranged by size:
3565 2810 2105 834 400 399 215
PciI A'CATG_T
Cuts at: 3029 6255 8784 3029
Size: 3226 2529 4573
Fragments arranged by size:
4573 3226 2529
PflMI CCAn_nnn'nTGG
Cuts at: 4131 6320 6609 4131
Size: 2189 289 7850
Fragments arranged by size:
7850 2189 289
PfoI T'CCnGG_A
Cuts at: 446 579 5597 7084 446
Size: 133 5018 1487 3690
Fragments arranged by size:
5018 3690 1487 133
PmeI GTTT'AAAC
Cuts at: 7327 7327
Size: 10328
PmlI CAC'GTG
Cuts at: 1842 6150 1842
Size: 4308 6020
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 1076 9467 9499 1044
Size: 32 8391 32 1873
Fragments arranged by size:
8391 1873 32 32
PpuMI rG'GwC_Cy
Cuts at: 573 2413 4976 573
Size: 1840 2563 5925
Fragments arranged by size:
5925 2563 1840
PshAI GACnn'nnGTC
Cuts at: 6012 6012
Size: 10328
PspOMI G'GGCC_C
Cuts at: 288 3414 3442 4237 5802 6171 6751 6806 288
Size: 3126 28 795 1565 369 580 55 3810
Fragments arranged by size:
3810 3126 1565 795 580 369 55 28
PspXI vC'TCGA_Gb
Cuts at: 6248 6271 6248
Size: 23 10305
PstI C_TGCA'G
Cuts at: 700 996 2139 2639 4889 7005 7375 8060 700
Size: 296 1143 500 2250 2116 370 685 2968
Fragments arranged by size:
2968 2250 2116 1143 685 500 370 296
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10328
PvuII CAG'CTG
Cuts at: 235 2800 4411 5891 6204 7753 8113 235
Size: 2565 1611 1480 313 1549 360 2450
Fragments arranged by size:
2565 2450 1611 1549 1480 360 313
RsrII CG'GwC_CG
Cuts at: 1391 8523 1391
Size: 7132 3196
SacI G_AGCT'C
Cuts at: 266 1001 1576 4816 6853 7342 266
Size: 735 575 3240 2037 489 3252
Fragments arranged by size:
3252 3240 2037 735 575 489
SacII CC_GC'GG
Cuts at: 5355 5355
Size: 10328
SalI G'TCGA_C
Cuts at: 7365 7365
Size: 10328
SanDI GG'GwC_CC
Cuts at: 2413 2413
Size: 10328
SapI GCTCTTCn'nnn_
Cuts at: 2257 2276 4256 8351 8561 2257
Size: 19 1980 4095 210 4024
Fragments arranged by size:
4095 4024 1980 210 19
SbfI CC_TGCA'GG
Cuts at: 7375 7375
Size: 10328
ScaI AGT'ACT
Cuts at: 694 1272 9845 694
Size: 578 8573 1177
Fragments arranged by size:
8573 1177 578
SfcI C'TryA_G
Cuts at: 33 696 992 2135 2635 4885 7001 7371 8056
Size: 663 296 1143 500 2250 2116 370 685
Cuts at: 8056 9049 9240 33
Size: 993 191 1121
Fragments arranged by size:
2250 2116 1143 1121 993 685 663 500
370 296 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 6814 6814
Size: 10328
SfoI GGC'GCC
Cuts at: 5750 7760 8008 5750
Size: 2010 248 8070
Fragments arranged by size:
8070 2010 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10328
SmaI CCC'GGG
Cuts at: 287 2328 3404 6811 6959 7350 287
Size: 2041 1076 3407 148 391 3265
Fragments arranged by size:
3407 3265 2041 1076 391 148
SmlI C'TyrA_G
Cuts at: 1209 2542 4353 4492 5979 6248 6271 7483 8398
Size: 1333 1811 139 1487 269 23 1212 915
Cuts at: 8398 8890 9152 1209
Size: 492 262 2385
Fragments arranged by size:
2385 1811 1487 1333 1212 915 492 269
262 139 23
SpeI A'CTAG_T
Cuts at: 67 970 67
Size: 903 9425
SphI G_CATG'C
Cuts at: 4966 5412 5916 7381 7881 4966
Size: 446 504 1465 500 7413
Fragments arranged by size:
7413 1465 504 500 446
SrfI GCCC'GGGC
Cuts at: 6959 6959
Size: 10328
StuI AGG'CCT
Cuts at: 854 3253 3267 6121 6293 6966 7186 854
Size: 2399 14 2854 172 673 220 3996
Fragments arranged by size:
3996 2854 2399 673 220 172 14
StyI C'CwwG_G
Cuts at: 107 1564 1976 2268 2405 2431 2967 3296 4046
Size: 1457 412 292 137 26 536 329 750
Cuts at: 4046 4146 4430 4475 4578 4671 4775 4799 5096
Size: 100 284 45 103 93 104 24 297
Cuts at: 5096 6377 6929 6984 7462 7816 107
Size: 1281 552 55 478 354 2619
Fragments arranged by size:
2619 1457 1281 750 552 536 478 412
354 329 297 292 284 137 104 103
100 93 55 45 26 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 859 1192 1432 2158 2829 3040 3068 3139
Size: 603 333 240 726 671 211 28 71
Cuts at: 3139 3616 3719 3755 4153 5446 5808 5847 7029
Size: 477 103 36 398 1293 362 39 1182
Cuts at: 7029 7911 8044 8503 8712 256
Size: 882 133 459 209 1872
Fragments arranged by size:
1872 1293 1182 882 726 671 603 477
459 398 362 333 240 211 209 133
103 71 39 36 28
TatI w'GTAC_w
Cuts at: 680 692 1270 3947 4109 5009 9843 680
Size: 12 578 2677 162 900 4834 1165
Fragments arranged by size:
4834 2677 1165 900 578 162 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 811 843 2503 2535 3839 3871 5117 5149 9845
Size: 32 1660 32 1304 32 1246 32 4696
Cuts at: 9845 9877 811
Size: 32 1262
Fragments arranged by size:
4696 1660 1304 1262 1246 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 8125 8125
Size: 10328
XbaI T'CTAG_A
Cuts at: 7359 7359
Size: 10328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 4053 6008 7146 7823 465
Size: 3588 1955 1138 677 2970
Fragments arranged by size:
3588 2970 1955 1138 677
XhoI C'TCGA_G
Cuts at: 2542 6248 6271 2542
Size: 3706 23 6599
Fragments arranged by size:
6599 3706 23
XmaI C'CCGG_G
Cuts at: 285 2326 3402 6809 6957 7348 285
Size: 2041 1076 3407 148 391 3265
Fragments arranged by size:
3407 3265 2041 1076 391 148
XmnI GAAnn'nnTTC
Cuts at: 6631 6631
Size: 10328
ZraI GAC'GTC
Cuts at: 5442 5442
Size: 10328
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AscI AvrII BmgBI BmtI BsiWI
BsmBI BssHII BstZ17I CspCI EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI PacI PsiI PsrI SexAI
SgrAI SnaBI SspI SwaI