Restriction map for
pF1KA1560
(Circular) (Six-base) MAPSORT of: pF1KA1560.seq Check: 9647 from: 1 to: 5597
pF1KA1560 5597 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 1126 2635 1126
Size: 1509 4088
Acc65I G'GTAC_C
Cuts at: 2613 2613
Size: 5597
AclI AA'CG_TT
Cuts at: 5437 5437
Size: 5597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 854 882 2245 2316 3422 3854 4601 4869
Size: 632 28 1363 71 1106 432 747 268
Cuts at: 4869 222
Size: 950
Fragments arranged by size:
1363 1106 950 747 632 432 268 71
28
AflIII A'CryG_T
Cuts at: 1057 1445 2949 4053 5030 1057
Size: 388 1504 1104 977 1624
Fragments arranged by size:
1624 1504 1104 977 388
AgeI A'CCGG_T
Cuts at: 1356 3985 1356
Size: 2629 2968
AleI CACnn'nnGTG
Cuts at: 197 490 197
Size: 293 5304
AlwNI CAG_nnn'CTG
Cuts at: 1725 2353 2902 4469 1725
Size: 628 549 1567 2853
Fragments arranged by size:
2853 1567 628 549
ApaLI G'TGCA_C
Cuts at: 4367 4367
Size: 5597
ApoI r'AATT_y
Cuts at: 337 722 768 1689 2601 4950 5206 337
Size: 385 46 921 912 2349 256 728
Fragments arranged by size:
2349 921 912 728 385 256 46
AseI AT'TA_AT
Cuts at: 20 1454 20
Size: 1434 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5597
AvaI C'yCGr_G
Cuts at: 1088 2617 1088
Size: 1529 4068
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 336 369 2341 2374 336
Size: 33 1972 33 3559
Fragments arranged by size:
3559 1972 33 33
BamHI G'GATC_C
Cuts at: 2622 2622
Size: 5597
BanI G'GyrC_C
Cuts at: 1996 2613 3027 3275 3310 1996
Size: 617 414 248 35 4283
Fragments arranged by size:
4283 617 414 248 35
BanII G_rGCy'C
Cuts at: 2611 3641 2611
Size: 1030 4567
BbeI G_GCGC'C
Cuts at: 3031 3279 3031
Size: 248 5349
BbsI GAAGACnn'nnnn_
Cuts at: 1634 1797 2192 1634
Size: 163 395 5039
Fragments arranged by size:
5039 395 163
BciVI GTATCCnnnnn_n'
Cuts at: 364 3495 4256 5597 364
Size: 3131 761 1341 364
Fragments arranged by size:
3131 1341 761 364
BclI T'GATC_A
Cuts at: 662 1934 3117 662
Size: 1272 1183 3142
Fragments arranged by size:
3142 1272 1183
BfrBI ATG'CAT
Cuts at: 1710 1710
Size: 5597
BglII A'GATC_T
Cuts at: 241 1040 3112 5149 241
Size: 799 2072 2037 689
Fragments arranged by size:
2072 2037 799 689
BlpI GC'TnA_GC
Cuts at: 1514 2123 2709 1514
Size: 609 586 4402
Fragments arranged by size:
4402 609 586
Bme1580I G_kGCm'C
Cuts at: 3222 3315 4371 3222
Size: 93 1056 4448
Fragments arranged by size:
4448 1056 93
BmgBI CAC'GTC
Cuts at: 1448 1448
Size: 5597
BmrI ACTGGGnnnn_n'
Cuts at: 344 683 1222 1412 1912 2809 2890 3225 5369
Size: 339 539 190 500 897 81 335 2144
Cuts at: 5369 344
Size: 572
Fragments arranged by size:
2144 897 572 539 500 339 335 190
81
BmtI G_CTAG'C
Cuts at: 1538 1538
Size: 5597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1009 3964 1009
Size: 2955 2642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2314 1399
Size: 915 4682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 2773 3652 4144 4442 760
Size: 2013 879 492 298 1915
Fragments arranged by size:
2013 1915 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2482 5301 2482
Size: 2819 2778
BsaAI yAC'GTr
Cuts at: 2950 2950
Size: 5597
BsaBI GATnn'nnATC
Cuts at: 6 3111 5148 6
Size: 3105 2037 455
Fragments arranged by size:
3105 2037 455
BsaHI Gr'CG_yC
Cuts at: 429 3028 3276 3978 5474 429
Size: 2599 248 702 1496 552
Fragments arranged by size:
2599 1496 702 552 248
BsaWI w'CCGG_w
Cuts at: 1356 2060 2786 3307 3985 4259 4406 4817 1356
Size: 704 726 521 678 274 147 411 2136
Fragments arranged by size:
2136 726 704 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 1969 1999 2261 2291 1372
Size: 30 567 30 262 30 4678
Fragments arranged by size:
4678 567 262 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1573 1573
Size: 5597
BseYI C'CCAG_C
Cuts at: 329 575 1725 1783 3022 3780 4357 4853 329
Size: 246 1150 58 1239 758 577 496 1073
Fragments arranged by size:
1239 1150 1073 758 577 496 246 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 300 104
Size: 196 5401
BsiEI CG_ry'CG
Cuts at: 104 3139 3185 4393 104
Size: 3035 46 1208 1308
Fragments arranged by size:
3035 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1089 2090 2249 2611 3389 3579 4371 1089
Size: 1001 159 362 778 190 792 2315
Fragments arranged by size:
2315 1001 792 778 362 190 159
BsmI GAATG_Cn'
Cuts at: 2069 2454 2069
Size: 385 5212
BsmBI CGTCTCn'nnnn_
Cuts at: 757 757
Size: 5597
Bsp1286I G_dGCh'C
Cuts at: 1089 2090 2249 2611 3222 3315 3389 3579 3641
Size: 1001 159 362 611 93 74 190 62
Cuts at: 3641 4371 1089
Size: 730 2315
Fragments arranged by size:
2315 1001 730 611 362 190 159 93
74 62
BspEI T'CCGG_A
Cuts at: 4817 4817
Size: 5597
BspHI T'CATG_A
Cuts at: 4773 4773
Size: 5597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2647 2861 3163 3544 2647
Size: 214 302 381 4700
Fragments arranged by size:
4700 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3889 3943 5427 5592 50
Size: 3839 54 1484 165 55
Fragments arranged by size:
3839 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 665 863 1833 3509 665
Size: 198 970 1676 2753
Fragments arranged by size:
2753 1676 970 198
BsrFI r'CCGG_y
Cuts at: 654 1356 3595 3776 3985 654
Size: 702 2239 181 209 2266
Fragments arranged by size:
2266 2239 702 209 181
BssSI C'ACGA_G
Cuts at: 1661 3868 4226 1661
Size: 2207 358 3032
Fragments arranged by size:
3032 2207 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1953 2370 2416 2974 1953
Size: 417 46 558 4576
Fragments arranged by size:
4576 558 417 46
BstBI TT'CG_AA
Cuts at: 1808 3958 1808
Size: 2150 3447
BstXI CCAn_nnnn'nTGG
Cuts at: 919 919
Size: 5597
BstYI r'GATC_y
Cuts at: 1 241 799 1040 2622 3089 3112 3447 3693
Size: 240 558 241 1582 467 23 335 246
Cuts at: 3693 4705 4791 4803 5149 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1582 1012 558 467 449 346 335 246
241 240 86 23 12
BtgI C'CryG_G
Cuts at: 107 411 996 107
Size: 304 585 4708
Fragments arranged by size:
4708 585 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3547 3728 3891 3547
Size: 181 163 5253
Fragments arranged by size:
5253 181 163
BtsI GCAGTG_nn'
Cuts at: 1529 2005 2137 2840 1529
Size: 476 132 703 4286
Fragments arranged by size:
4286 703 476 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1548 1583 2316 2351 1548
Size: 35 733 35 4794
Fragments arranged by size:
4794 733 35 35
DraI TTT'AAA
Cuts at: 254 2596 254
Size: 2342 3255
DraIII CAC_nnn'GTG
Cuts at: 1569 1569
Size: 5597
DrdI GACnn_nn'nnGTC
Cuts at: 1907 3303 4161 1907
Size: 1396 858 3343
Fragments arranged by size:
3343 1396 858
EaeI y'GGCC_r
Cuts at: 1964 3182 3356 3747 3774 5119 1964
Size: 1218 174 391 27 1345 2442
Fragments arranged by size:
2442 1345 1218 391 174 27
EagI C'GGCC_G
Cuts at: 3182 3182
Size: 5597
EarI CTCTTCn'nnn_
Cuts at: 841 3620 3830 841
Size: 2779 210 2608
Fragments arranged by size:
2779 2608 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3805 4115 4261 5407 3805
Size: 310 146 1146 3995
Fragments arranged by size:
3995 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2609 2609
Size: 5597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 854 882 1399 2245 2314 2316 3422 3854
Size: 632 28 517 846 69 2 1106 432
Cuts at: 3854 4601 4869 222
Size: 747 268 950
Fragments arranged by size:
1106 950 846 747 632 517 432 268
69 28 2
EcoO109I rG'GnC_Cy
Cuts at: 1909 2736 1909
Size: 827 4770
EcoRI G'AATT_C
Cuts at: 2601 2601
Size: 5597
EcoRV GAT'ATC
Cuts at: 2106 2106
Size: 5597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 754 3616 754
Size: 2862 2735
FspI TGC'GCA
Cuts at: 3378 3378
Size: 5597
HaeII r_GCGC'y
Cuts at: 3031 3279 4301 5280 3031
Size: 248 1022 979 3348
Fragments arranged by size:
3348 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 929 961 1108 1140 1491 1523 3439
Size: 32 679 32 147 32 351 32 1916
Cuts at: 3439 3469 3471 3501 218
Size: 30 2 30 2314
Fragments arranged by size:
2314 1916 679 351 147 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 597 736 2636 597
Size: 139 1900 3558
Fragments arranged by size:
3558 1900 139
HindIII A'AGCT_T
Cuts at: 916 2143 916
Size: 1227 4370
HpaI GTT'AAC
Cuts at: 597 597
Size: 5597
KasI G'GCGC_C
Cuts at: 3027 3275 3027
Size: 248 5349
KpnI G_GTAC'C
Cuts at: 2617 2617
Size: 5597
MfeI C'AATT_G
Cuts at: 533 533
Size: 5597
MluI A'CGCG_T
Cuts at: 5030 5030
Size: 5597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 259 276 1143 4268 4452 259
Size: 17 867 3125 184 1404
Fragments arranged by size:
3125 1404 867 184 17
MscI TGG'CCA
Cuts at: 3358 3358
Size: 5597
MslI CAynn'nnrTG
Cuts at: 197 490 1199 3586 3713 197
Size: 293 709 2387 127 2081
Fragments arranged by size:
2387 2081 709 293 127
MspA1I CmG'CkG
Cuts at: 413 1722 1950 2708 3022 3382 4395 4640 413
Size: 1309 228 758 314 360 1013 245 1370
Fragments arranged by size:
1370 1309 1013 758 360 314 245 228
NaeI GCC'GGC
Cuts at: 3778 3778
Size: 5597
NarI GG'CG_CC
Cuts at: 3028 3276 3028
Size: 248 5349
NcoI C'CATG_G
Cuts at: 107 996 107
Size: 889 4708
NgoMIV G'CCGG_C
Cuts at: 3776 3776
Size: 5597
NheI G'CTAG_C
Cuts at: 1534 1534
Size: 5597
NsiI A_TGCA'T
Cuts at: 1712 1712
Size: 5597
NspI r_CATG'y
Cuts at: 480 1714 2650 3150 4057 480
Size: 1234 936 500 907 2020
Fragments arranged by size:
2020 1234 936 907 500
PciI A'CATG_T
Cuts at: 4053 4053
Size: 5597
PflMI CCAn_nnn'nTGG
Cuts at: 295 295
Size: 5597
PfoI T'CCnGG_A
Cuts at: 1046 1046
Size: 5597
PmeI GTTT'AAAC
Cuts at: 2596 2596
Size: 5597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 4736
Size: 32 5565
PpuMI rG'GwC_Cy
Cuts at: 1909 1909
Size: 5597
PshAI GACnn'nnGTC
Cuts at: 1101 1101
Size: 5597
PstI C_TGCA'G
Cuts at: 2644 3329 2644
Size: 685 4912
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5597
PvuII CAG'CTG
Cuts at: 1722 1950 3022 3382 1722
Size: 228 1072 360 3937
Fragments arranged by size:
3937 1072 360 228
RsrII CG'GwC_CG
Cuts at: 3792 3792
Size: 5597
SacI G_AGCT'C
Cuts at: 2611 2611
Size: 5597
SacII CC_GC'GG
Cuts at: 414 414
Size: 5597
SalI G'TCGA_C
Cuts at: 2634 2634
Size: 5597
SanDI GG'GwC_CC
Cuts at: 1909 1909
Size: 5597
SapI GCTCTTCn'nnn_
Cuts at: 3620 3830 3620
Size: 210 5387
SbfI CC_TGCA'GG
Cuts at: 2644 2644
Size: 5597
ScaI AGT'ACT
Cuts at: 5114 5114
Size: 5597
SfcI C'TryA_G
Cuts at: 33 981 1587 1821 2301 2640 3325 4318 4509
Size: 948 606 234 480 339 685 993 191
Cuts at: 4509 33
Size: 1121
Fragments arranged by size:
1121 993 948 685 606 480 339 234
191
SfoI GGC'GCC
Cuts at: 3029 3277 3029
Size: 248 5349
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5597
SmaI CCC'GGG
Cuts at: 2619 2619
Size: 5597
SmlI C'TyrA_G
Cuts at: 739 2752 3667 4159 4421 739
Size: 2013 915 492 262 1915
Fragments arranged by size:
2013 1915 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5597
SphI G_CATG'C
Cuts at: 1714 2650 3150 1714
Size: 936 500 4161
Fragments arranged by size:
4161 936 500
SspI AAT'ATT
Cuts at: 1333 1333
Size: 5597
StuI AGG'CCT
Cuts at: 2299 2299
Size: 5597
StyI C'CwwG_G
Cuts at: 107 129 996 2240 2280 2731 3085 107
Size: 22 867 1244 40 451 354 2619
Fragments arranged by size:
2619 1244 867 451 354 40 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 665 669 1762 3180 3313 3772 3981 665
Size: 4 1093 1418 133 459 209 2281
Fragments arranged by size:
2281 1418 1093 459 209 133 4
TatI w'GTAC_w
Cuts at: 522 5112 522
Size: 4590 1007
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5114 5146 5114
Size: 32 5565
Tth111I GACn'n_nGTC
Cuts at: 3394 3394
Size: 5597
XbaI T'CTAG_A
Cuts at: 2562 2628 2562
Size: 66 5531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1566 1722 3092 1566
Size: 156 1370 4071
Fragments arranged by size:
4071 1370 156
XmaI C'CCGG_G
Cuts at: 2617 2617
Size: 5597
XmnI GAAnn'nnTTC
Cuts at: 1016 2570 1016
Size: 1554 4043
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI Bpu10I BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoNI FseI FspAI
NdeI NotI NruI PacI PasI PmlI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI