Restriction map for
pF1KA1565
(Circular) (Six-base) MAPSORT of: pF1KA1565.seq Check: 3786 from: 1 to: 6938
pF1KA1565 6938 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2258 2258
Size: 6938
AccI GT'mk_AC
Cuts at: 3976 3976
Size: 6938
Acc65I G'GTAC_C
Cuts at: 3652 3954 3652
Size: 302 6636
AclI AA'CG_TT
Cuts at: 2901 6778 2901
Size: 3877 3061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 491 1105 1603 1913 2128 2326 2479 2975
Size: 238 614 498 310 215 198 153 496
Cuts at: 2975 3379 4763 5195 5548 6210 253
Size: 404 1384 432 353 662 981
Fragments arranged by size:
1384 981 662 614 498 496 432 404
353 310 238 215 198 153
AflIII A'CryG_T
Cuts at: 221 4290 6090 6371 221
Size: 4069 1800 281 788
Fragments arranged by size:
4069 1800 788 281
AleI CACnn'nnGTG
Cuts at: 2926 2926
Size: 6938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3200 3898 3200
Size: 698 6240
AlwNI CAG_nnn'CTG
Cuts at: 1440 3516 3913 4243 5681 1440
Size: 2076 397 330 1438 2697
Fragments arranged by size:
2697 2076 1438 397 330
ApaI G_GGCC'C
Cuts at: 3423 3423
Size: 6938
ApaLI G'TGCA_C
Cuts at: 1793 5776 1793
Size: 3983 2955
ApoI r'AATT_y
Cuts at: 1626 2535 2685 3273 3753 3942 6291 6547 1626
Size: 909 150 588 480 189 2349 256 2017
Fragments arranged by size:
2349 2017 909 588 480 256 189 150
AseI AT'TA_AT
Cuts at: 20 319 20
Size: 299 6639
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6938
AvaI C'yCGr_G
Cuts at: 439 778 3584 3958 439
Size: 339 2806 374 3419
Fragments arranged by size:
3419 2806 374 339
BamHI G'GATC_C
Cuts at: 3963 3963
Size: 6938
BanI G'GyrC_C
Cuts at: 905 3652 3954 4368 4616 4651 905
Size: 2747 302 414 248 35 3192
Fragments arranged by size:
3192 2747 414 302 248 35
BanII G_rGCy'C
Cuts at: 1874 1886 1994 2288 2471 2951 3423 3452 3952
Size: 12 108 294 183 480 472 29 500
Cuts at: 3952 4982 1874
Size: 1030 3830
Fragments arranged by size:
3830 1030 500 480 472 294 183 108
29 12
BbeI G_GCGC'C
Cuts at: 4372 4620 4372
Size: 248 6690
BbsI GAAGACnn'nnnn_
Cuts at: 556 981 2924 3287 556
Size: 425 1943 363 4207
Fragments arranged by size:
4207 1943 425 363
BbvCI CC'TCA_GC
Cuts at: 2263 2263
Size: 6938
BciVI GTATCCnnnnn_n'
Cuts at: 4836 5892 6938 4836
Size: 1056 1046 4836
Fragments arranged by size:
4836 1056 1046
BclI T'GATC_A
Cuts at: 1323 3702 4458 1323
Size: 2379 756 3803
Fragments arranged by size:
3803 2379 756
BfrBI ATG'CAT
Cuts at: 1021 1021
Size: 6938
BglII A'GATC_T
Cuts at: 1272 4453 6490 1272
Size: 3181 2037 1720
Fragments arranged by size:
3181 2037 1720
BlpI GC'TnA_GC
Cuts at: 389 4050 389
Size: 3661 3277
Bme1580I G_kGCm'C
Cuts at: 181 1797 3423 4563 4656 5780 181
Size: 1616 1626 1140 93 1124 1339
Fragments arranged by size:
1626 1616 1339 1140 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 241 4150 4231 4566 6710 241
Size: 3909 81 335 2144 469
Fragments arranged by size:
3909 2144 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 793 5305 793
Size: 4512 2426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1753 2008 2959 3034 3526 1753
Size: 255 951 75 492 5165
Fragments arranged by size:
5165 951 492 255 75
Bpu10I CC'TnA_GC
Cuts at: 1810 2263 1810
Size: 453 6485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 1279 2149 2251 2272 2294 2464 4114 4993
Size: 1149 870 102 21 22 170 1650 879
Cuts at: 4993 5707 6005 130
Size: 714 298 1063
Fragments arranged by size:
1650 1149 1063 879 870 714 298 170
102 22 21
BsaI GGTCTCn'nnnn_
Cuts at: 735 6642 735
Size: 5907 1031
BsaAI yAC'GTr
Cuts at: 4291 4291
Size: 6938
BsaBI GATnn'nnATC
Cuts at: 6 2511 4452 6489 6
Size: 2505 1941 2037 455
Fragments arranged by size:
2505 2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 3929 4369 4617 5319 6815 3929
Size: 440 248 702 1496 4052
Fragments arranged by size:
4052 1496 702 440 248
BsaWI w'CCGG_w
Cuts at: 716 4127 4648 5326 5737 5884 6158 716
Size: 3411 521 678 411 147 274 1496
Fragments arranged by size:
3411 1496 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 475 580 2215 2218 2248 2467 250
Size: 225 105 1635 3 30 219 4721
Fragments arranged by size:
4721 1635 225 219 105 30 3
BseYI C'CCAG_C
Cuts at: 522 3416 4363 5121 5786 6194 522
Size: 2894 947 758 665 408 1266
Fragments arranged by size:
2894 1266 947 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1758 2932 3016 3310 3894 1758
Size: 1174 84 294 584 4802
Fragments arranged by size:
4802 1174 584 294 84
BsiEI CG_ry'CG
Cuts at: 104 4480 4526 5756 104
Size: 4376 46 1230 1286
Fragments arranged by size:
4376 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 545 1797 1811 1994 2165 2288 2471 2951 3952
Size: 1252 14 183 171 123 183 480 1001
Cuts at: 3952 4730 4920 5780 545
Size: 778 190 860 1703
Fragments arranged by size:
1703 1252 1001 860 778 480 190 183
183 171 123 14
BsmI GAATG_Cn'
Cuts at: 621 1175 1903 3405 621
Size: 554 728 1502 4154
Fragments arranged by size:
4154 1502 728 554
Bsp1286I G_dGCh'C
Cuts at: 181 545 1797 1811 1874 1886 1994 2165 2288
Size: 364 1252 14 63 12 108 171 123
Cuts at: 2288 2471 2951 3423 3452 3952 4563 4656 4730
Size: 183 480 472 29 500 611 93 74
Cuts at: 4730 4920 4982 5780 181
Size: 190 62 798 1339
Fragments arranged by size:
1339 1252 798 611 500 480 472 364
190 183 171 123 108 93 74 63
62 29 14 12
BspHI T'CATG_A
Cuts at: 2178 5370 2178
Size: 3192 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2258 3311 3988 4202 4504 4885 2258
Size: 1053 677 214 302 381 4311
Fragments arranged by size:
4311 1053 677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1344 1731 2325 5230 5284 6768 6933 50
Size: 1294 387 594 2905 54 1484 165 55
Fragments arranged by size:
2905 1484 1294 594 387 165 55 54
BsrDI GCAATG_nn'
Cuts at: 968 4850 968
Size: 3882 3056
BsrFI r'CCGG_y
Cuts at: 526 4936 5117 526
Size: 4410 181 2347
Fragments arranged by size:
4410 2347 181
BssSI C'ACGA_G
Cuts at: 5209 5917 5209
Size: 708 6230
BstAPI GCAn_nnn'nTGC
Cuts at: 1440 4315 1440
Size: 2875 4063
BstBI TT'CG_AA
Cuts at: 5299 5299
Size: 6938
BstEII G'GTnAC_C
Cuts at: 706 1161 1657 706
Size: 455 496 5987
Fragments arranged by size:
5987 496 455
BstYI r'GATC_y
Cuts at: 1 631 1079 1272 1436 2614 3963 4430 4453
Size: 630 448 193 164 1178 1349 467 23
Cuts at: 4453 4788 5034 5340 5352 5438 6490 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1349 1178 1052 630 467 449 448 335
306 246 193 164 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1363 3914 1363
Size: 2551 4387
BtgI C'CryG_G
Cuts at: 107 455 2036 3548 107
Size: 348 1581 1512 3497
Fragments arranged by size:
3497 1581 1512 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4888 5069 5232 4888
Size: 181 163 6594
Fragments arranged by size:
6594 181 163
BtsI GCAGTG_nn'
Cuts at: 543 567 1680 1741 2165 3921 4181 543
Size: 24 1113 61 424 1756 260 3300
Fragments arranged by size:
3300 1756 1113 424 260 61 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2408 2443 2408
Size: 35 6903
DraI TTT'AAA
Cuts at: 309 849 1538 2567 2690 3362 3937 309
Size: 540 689 1029 123 672 575 3310
Fragments arranged by size:
3310 1029 689 672 575 540 123
DraIII CAC_nnn'GTG
Cuts at: 628 628
Size: 6938
DrdI GACnn_nn'nnGTC
Cuts at: 4644 5988 4644
Size: 1344 5594
EaeI y'GGCC_r
Cuts at: 1193 1334 4523 4697 5088 5115 6460 1193
Size: 141 3189 174 391 27 1345 1671
Fragments arranged by size:
3189 1671 1345 391 174 141 27
EagI C'GGCC_G
Cuts at: 4523 4523
Size: 6938
EarI CTCTTCn'nnn_
Cuts at: 653 1049 1373 1496 1691 2357 4961 5171 653
Size: 396 324 123 195 666 2604 210 2420
Fragments arranged by size:
2604 2420 666 396 324 210 195 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1362 2959 5146 5888 6034 6748 1362
Size: 1597 2187 742 146 714 1552
Fragments arranged by size:
2187 1597 1552 742 714 146
EcoICRI GAG'CTC
Cuts at: 1992 2286 2469 2949 3950 1992
Size: 294 183 480 1001 4980
Fragments arranged by size:
4980 1001 480 294 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 491 1105 1603 1753 1913 2008 2128 2326
Size: 238 614 498 150 160 95 120 198
Cuts at: 2326 2479 2959 2975 3034 3379 3526 4763 5195
Size: 153 480 16 59 345 147 1237 432
Cuts at: 5195 5548 6210 253
Size: 353 662 981
Fragments arranged by size:
1237 981 662 614 498 480 432 353
345 238 198 160 153 150 147 120
95 59 16
EcoNI CCTnn'n_nnAGG
Cuts at: 193 1444 193
Size: 1251 5687
EcoO109I rG'GnC_Cy
Cuts at: 185 431 3420 4077 185
Size: 246 2989 657 3046
Fragments arranged by size:
3046 2989 657 246
EcoRI G'AATT_C
Cuts at: 3753 3942 3753
Size: 189 6749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 4957 2245
Size: 2712 4226
FspI TGC'GCA
Cuts at: 4719 4719
Size: 6938
HaeII r_GCGC'y
Cuts at: 2354 4372 4620 5850 6621 2354
Size: 2018 248 1230 771 2671
Fragments arranged by size:
2671 2018 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1421 1453 1976 2008 2292 2324 3420 3452 4780
Size: 32 523 32 284 32 1096 32 1328
Cuts at: 4780 4810 4812 4842 1421
Size: 30 2 30 3517
Fragments arranged by size:
3517 1328 1096 523 284 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3977 3977
Size: 6938
HindIII A'AGCT_T
Cuts at: 2227 2316 2521 2227
Size: 89 205 6644
Fragments arranged by size:
6644 205 89
KasI G'GCGC_C
Cuts at: 4368 4616 4368
Size: 248 6690
KpnI G_GTAC'C
Cuts at: 3656 3958 3656
Size: 302 6636
MfeI C'AATT_G
Cuts at: 2728 3601 2728
Size: 873 6065
MluI A'CGCG_T
Cuts at: 6371 6371
Size: 6938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 3467 3834 5697 5881 1205
Size: 2262 367 1863 184 2262
Fragments arranged by size:
2262 2262 1863 367 184
MscI TGG'CCA
Cuts at: 1195 4699 1195
Size: 3504 3434
MslI CAynn'nnrTG
Cuts at: 2926 3224 4927 5054 2926
Size: 298 1703 127 4810
Fragments arranged by size:
4810 1703 298 127
MspA1I CmG'CkG
Cuts at: 1859 2025 2045 2415 3030 3246 3516 4049 4363
Size: 166 20 370 615 216 270 533 314
Cuts at: 4363 4723 5507 5752 1859
Size: 360 784 245 3045
Fragments arranged by size:
3045 784 615 533 370 360 314 270
245 216 166 20
NaeI GCC'GGC
Cuts at: 5119 5119
Size: 6938
NarI GG'CG_CC
Cuts at: 4369 4617 4369
Size: 248 6690
NcoI C'CATG_G
Cuts at: 107 3548 107
Size: 3441 3497
NdeI CA'TA_TG
Cuts at: 1972 1972
Size: 6938
NgoMIV G'CCGG_C
Cuts at: 5117 5117
Size: 6938
NsiI A_TGCA'T
Cuts at: 1023 1023
Size: 6938
NspI r_CATG'y
Cuts at: 225 3991 4491 6094 225
Size: 3766 500 1603 1069
Fragments arranged by size:
3766 1603 1069 500
PasI CC'CwG_GG
Cuts at: 189 189
Size: 6938
PciI A'CATG_T
Cuts at: 221 6090 221
Size: 5869 1069
PflMI CCAn_nnn'nTGG
Cuts at: 2730 3549 2730
Size: 819 6119
PfoI T'CCnGG_A
Cuts at: 1514 3313 1514
Size: 1799 5139
PmeI GTTT'AAAC
Cuts at: 3937 3937
Size: 6938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1868 1900 5384 5416 1868
Size: 32 3484 32 3390
Fragments arranged by size:
3484 3390 32 32
PpuMI rG'GwC_Cy
Cuts at: 185 431 185
Size: 246 6692
PshAI GACnn'nnGTC
Cuts at: 429 429
Size: 6938
PspOMI G'GGCC_C
Cuts at: 3419 3419
Size: 6938
PspXI vC'TCGA_Gb
Cuts at: 778 778
Size: 6938
PstI C_TGCA'G
Cuts at: 257 538 1445 2255 2667 3985 4670 257
Size: 281 907 810 412 1318 685 2525
Fragments arranged by size:
2525 1318 907 810 685 412 281
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6938
PvuII CAG'CTG
Cuts at: 2025 2045 2415 3030 3246 3516 4363 4723 2025
Size: 20 370 615 216 270 847 360 4240
Fragments arranged by size:
4240 847 615 370 360 270 216 20
RsrII CG'GwC_CG
Cuts at: 5133 5133
Size: 6938
SacI G_AGCT'C
Cuts at: 1994 2288 2471 2951 3952 1994
Size: 294 183 480 1001 4980
Fragments arranged by size:
4980 1001 480 294 183
SalI G'TCGA_C
Cuts at: 3975 3975
Size: 6938
SanDI GG'GwC_CC
Cuts at: 185 185
Size: 6938
SapI GCTCTTCn'nnn_
Cuts at: 4961 5171 4961
Size: 210 6728
SbfI CC_TGCA'GG
Cuts at: 2255 3985 2255
Size: 1730 5208
ScaI AGT'ACT
Cuts at: 6455 6455
Size: 6938
SfcI C'TryA_G
Cuts at: 33 253 534 1441 2251 2663 3981 4666 5634
Size: 220 281 907 810 412 1318 685 968
Cuts at: 5634 5825 33
Size: 191 1146
Fragments arranged by size:
1318 1146 968 907 810 685 412 281
220 191
SfoI GGC'GCC
Cuts at: 4370 4618 4370
Size: 248 6690
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6938
SmaI CCC'GGG
Cuts at: 3586 3960 3586
Size: 374 6564
SmlI C'TyrA_G
Cuts at: 145 778 1294 2164 2230 2287 2309 2443 4093
Size: 633 516 870 66 57 22 134 1650
Cuts at: 4093 5008 5722 5984 145
Size: 915 714 262 1099
Fragments arranged by size:
1650 1099 915 870 714 633 516 262
134 66 57 22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6938
SphI G_CATG'C
Cuts at: 3991 4491 3991
Size: 500 6438
StuI AGG'CCT
Cuts at: 1090 2339 3800 1090
Size: 1249 1461 4228
Fragments arranged by size:
4228 1461 1249
StyI C'CwwG_G
Cuts at: 107 1091 3548 4072 4426 107
Size: 984 2457 524 354 2619
Fragments arranged by size:
2619 2457 984 524 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4521 4654 5113 5322 4521
Size: 133 459 209 6137
Fragments arranged by size:
6137 459 209 133
TatI w'GTAC_w
Cuts at: 1355 1382 6453 1355
Size: 27 5071 1840
Fragments arranged by size:
5071 1840 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 684 716 3902 3934 6455 6487 684
Size: 32 3186 32 2521 32 1135
Fragments arranged by size:
3186 2521 1135 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4735 4735
Size: 6938
XbaI T'CTAG_A
Cuts at: 1569 3969 1569
Size: 2400 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 4433 114
Size: 4319 2619
XhoI C'TCGA_G
Cuts at: 778 778
Size: 6938
XmaI C'CCGG_G
Cuts at: 3584 3958 3584
Size: 374 6564
XmnI GAAnn'nnTTC
Cuts at: 359 2339 2522 3266 3779 359
Size: 1980 183 744 513 3518
Fragments arranged by size:
3518 1980 744 513 183
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsaXI BsiWI BsmBI
BspEI BsrGI BssHII BstXI BstZ17I ClaI EcoRV FseI
FspAI HpaI NheI NotI NruI PacI PmlI PsiI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI