Restriction map for
pF1KA1567
(Circular) (Six-base) MAPSORT of: pF1KA1567.seq Check: 8854 from: 1 to: 5696
pF1KA1567 5696 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1218 2734 1218
Size: 1516 4180
Acc65I G'GTAC_C
Cuts at: 2712 2712
Size: 5696
AclI AA'CG_TT
Cuts at: 5536 5536
Size: 5696
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1798 3521 3953 4306 4968 481
Size: 1317 1723 432 353 662 1209
Fragments arranged by size:
1723 1317 1209 662 432 353
AflIII A'CryG_T
Cuts at: 374 1683 3048 4848 5129 374
Size: 1309 1365 1800 281 941
Fragments arranged by size:
1800 1365 1309 941 281
AleI CACnn'nnGTG
Cuts at: 604 2317 2482 604
Size: 1713 165 3818
Fragments arranged by size:
3818 1713 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1427 1427
Size: 5696
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1579 1611 2379 2411 1579
Size: 32 768 32 4864
Fragments arranged by size:
4864 768 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2037 2457 2518 3001 4439 2037
Size: 420 61 483 1438 3294
Fragments arranged by size:
3294 1438 483 420 61
ApaLI G'TGCA_C
Cuts at: 2490 4534 2490
Size: 2044 3652
ApoI r'AATT_y
Cuts at: 847 1270 2378 2700 5049 5305 847
Size: 423 1108 322 2349 256 1238
Fragments arranged by size:
2349 1238 1108 423 322 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5696
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5696
AvaI C'yCGr_G
Cuts at: 1099 2716 1099
Size: 1617 4079
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 296 329 296
Size: 33 5663
BamHI G'GATC_C
Cuts at: 2625 2721 2625
Size: 96 5600
BanI G'GyrC_C
Cuts at: 2712 3126 3374 3409 2712
Size: 414 248 35 4999
Fragments arranged by size:
4999 414 248 35
BanII G_rGCy'C
Cuts at: 759 1307 2009 2710 3740 759
Size: 548 702 701 1030 2715
Fragments arranged by size:
2715 1030 702 701 548
BbeI G_GCGC'C
Cuts at: 3130 3378 3130
Size: 248 5448
BbsI GAAGACnn'nnnn_
Cuts at: 1750 1750
Size: 5696
BciVI GTATCCnnnnn_n'
Cuts at: 3594 4650 5696 3594
Size: 1056 1046 3594
Fragments arranged by size:
3594 1056 1046
BclI T'GATC_A
Cuts at: 1169 3216 1169
Size: 2047 3649
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5696
BglII A'GATC_T
Cuts at: 1186 1994 3211 5248 1186
Size: 808 1217 2037 1634
Fragments arranged by size:
2037 1634 1217 808
BlpI GC'TnA_GC
Cuts at: 758 2808 758
Size: 2050 3646
Bme1580I G_kGCm'C
Cuts at: 527 2494 3321 3414 4538 527
Size: 1967 827 93 1124 1685
Fragments arranged by size:
1967 1685 1124 827 93
BmrI ACTGGGnnnn_n'
Cuts at: 598 2908 2989 3324 5468 598
Size: 2310 81 335 2144 826
Fragments arranged by size:
2310 2144 826 335 81
BmtI G_CTAG'C
Cuts at: 140 140
Size: 5696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 4063 2510
Size: 1553 4143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2434 2434
Size: 5696
Bpu10I CC'TnA_GC
Cuts at: 2642 2642
Size: 5696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2872 3751 4465 4763 2872
Size: 879 714 298 3805
Fragments arranged by size:
3805 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 690 874 5400 690
Size: 184 4526 986
Fragments arranged by size:
4526 986 184
BsaAI yAC'GTr
Cuts at: 3049 3049
Size: 5696
BsaBI GATnn'nnATC
Cuts at: 6 239 2573 3210 5247 6
Size: 233 2334 637 2037 455
Fragments arranged by size:
2334 2037 637 455 233
BsaHI Gr'CG_yC
Cuts at: 3127 3375 4077 5573 3127
Size: 248 702 1496 3250
Fragments arranged by size:
3250 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2885 3406 4084 4495 4642 4916 2885
Size: 521 678 411 147 274 3665
Fragments arranged by size:
3665 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 571 601 658 688 1579 1609 1859 1889 571
Size: 30 57 30 891 30 250 30 4378
Fragments arranged by size:
4378 891 250 57 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1999 1999
Size: 5696
BseYI C'CCAG_C
Cuts at: 117 520 836 1887 1948 2669 3121 3879 4544
Size: 403 316 1051 61 721 452 758 665
Cuts at: 4544 4952 117
Size: 408 861
Fragments arranged by size:
1051 861 758 721 665 452 408 403
316 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1595 1996 2285 1595
Size: 401 289 5006
Fragments arranged by size:
5006 401 289
BsiEI CG_ry'CG
Cuts at: 104 2622 3238 3284 4514 104
Size: 2518 616 46 1230 1286
Fragments arranged by size:
2518 1286 1230 616 46
BsiHKAI G_wGCw'C
Cuts at: 1307 2009 2494 2710 3488 3678 4538 1307
Size: 702 485 216 778 190 860 2465
Fragments arranged by size:
2465 860 778 702 485 216 190
BsmI GAATG_Cn'
Cuts at: 1535 1535
Size: 5696
Bsp1286I G_dGCh'C
Cuts at: 527 759 1307 2009 2494 2710 3321 3414 3488
Size: 232 548 702 485 216 611 93 74
Cuts at: 3488 3678 3740 4538 527
Size: 190 62 798 1685
Fragments arranged by size:
1685 798 702 611 548 485 232 216
190 93 74 62
BspHI T'CATG_A
Cuts at: 2577 4128 2577
Size: 1551 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 708 2746 2960 3262 3643 708
Size: 2038 214 302 381 2761
Fragments arranged by size:
2761 2038 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1870 3988 4042 5526 5691 50
Size: 1820 2118 54 1484 165 55
Fragments arranged by size:
2118 1820 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1913 2304 3608 1913
Size: 391 1304 4001
Fragments arranged by size:
4001 1304 391
BsrFI r'CCGG_y
Cuts at: 1952 2207 3694 3875 1952
Size: 255 1487 181 3773
Fragments arranged by size:
3773 1487 255 181
BsrGI T'GTAC_A
Cuts at: 377 994 377
Size: 617 5079
BssSI C'ACGA_G
Cuts at: 3967 4675 3967
Size: 708 4988
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1573 2527 3073 1189
Size: 384 954 546 3812
Fragments arranged by size:
3812 954 546 384
BstBI TT'CG_AA
Cuts at: 2175 4057 2175
Size: 1882 3814
BstEII G'GTnAC_C
Cuts at: 882 882
Size: 5696
BstYI r'GATC_y
Cuts at: 1 1186 1994 2625 2721 3188 3211 3546 3792
Size: 1185 808 631 96 467 23 335 246
Cuts at: 3792 4098 4110 4196 5248 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1185 1052 808 631 467 449 335 306
246 96 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2630 2630
Size: 5696
BtgI C'CryG_G
Cuts at: 107 2115 107
Size: 2008 3688
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1143 3646 3827 3990 1143
Size: 2503 181 163 2849
Fragments arranged by size:
2849 2503 181 163
BtsI GCAGTG_nn'
Cuts at: 2435 2939 2435
Size: 504 5192
DraI TTT'AAA
Cuts at: 462 2695 462
Size: 2233 3463
DraIII CAC_nnn'GTG
Cuts at: 1573 1718 1720 1573
Size: 145 2 5549
Fragments arranged by size:
5549 145 2
DrdI GACnn_nn'nnGTC
Cuts at: 3402 4746 3402
Size: 1344 4352
EaeI y'GGCC_r
Cuts at: 937 1709 1941 2155 2209 2619 3281 3455 3846
Size: 772 232 214 54 410 662 174 391
Cuts at: 3846 3873 5218 937
Size: 27 1345 1415
Fragments arranged by size:
1415 1345 772 662 410 391 232 214
174 54 27
EagI C'GGCC_G
Cuts at: 2619 3281 2619
Size: 662 5034
EarI CTCTTCn'nnn_
Cuts at: 437 1202 1222 2263 3719 3929 437
Size: 765 20 1041 1456 210 2204
Fragments arranged by size:
2204 1456 1041 765 210 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3904 4646 4792 5506 3904
Size: 742 146 714 4094
Fragments arranged by size:
4094 742 714 146
EcoICRI GAG'CTC
Cuts at: 1305 2007 2708 1305
Size: 702 701 4293
Fragments arranged by size:
4293 702 701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1798 2434 3521 3953 4306 4968 481
Size: 1317 636 1087 432 353 662 1209
Fragments arranged by size:
1317 1209 1087 662 636 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 591 653 2484 591
Size: 62 1831 3803
Fragments arranged by size:
3803 1831 62
EcoO109I rG'GnC_Cy
Cuts at: 121 1832 2034 2835 121
Size: 1711 202 801 2982
Fragments arranged by size:
2982 1711 801 202
EcoRI G'AATT_C
Cuts at: 2378 2700 2378
Size: 322 5374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3715 3715
Size: 5696
FspI TGC'GCA
Cuts at: 3477 3477
Size: 5696
HaeII r_GCGC'y
Cuts at: 118 3130 3378 4608 5379 118
Size: 3012 248 1230 771 435
Fragments arranged by size:
3012 1230 771 435 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 807 839 1737 1769 1859 1891 3538 3568 3570
Size: 32 898 32 90 32 1647 30 2
Cuts at: 3570 3600 807
Size: 30 2903
Fragments arranged by size:
2903 1647 898 90 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 288 2735 288
Size: 2447 3249
HindIII A'AGCT_T
Cuts at: 512 512
Size: 5696
KasI G'GCGC_C
Cuts at: 3126 3374 3126
Size: 248 5448
KpnI G_GTAC'C
Cuts at: 2716 2716
Size: 5696
MfeI C'AATT_G
Cuts at: 398 398
Size: 5696
MluI A'CGCG_T
Cuts at: 5129 5129
Size: 5696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 159 1668 4455 4639 159
Size: 1509 2787 184 1216
Fragments arranged by size:
2787 1509 1216 184
MscI TGG'CCA
Cuts at: 1711 1943 2157 3457 1711
Size: 232 214 1300 3950
Fragments arranged by size:
3950 1300 232 214
MslI CAynn'nnrTG
Cuts at: 248 530 604 2317 2480 2482 2582 3685 3812
Size: 282 74 1713 163 2 100 1103 127
Cuts at: 3812 248
Size: 2132
Fragments arranged by size:
2132 1713 1103 282 163 127 100 74
2
MspA1I CmG'CkG
Cuts at: 1503 2469 2807 3121 3481 4265 4510 1503
Size: 966 338 314 360 784 245 2689
Fragments arranged by size:
2689 966 784 360 338 314 245
NaeI GCC'GGC
Cuts at: 3877 3877
Size: 5696
NarI GG'CG_CC
Cuts at: 3127 3375 3127
Size: 248 5448
NcoI C'CATG_G
Cuts at: 107 107
Size: 5696
NgoMIV G'CCGG_C
Cuts at: 3875 3875
Size: 5696
NheI G'CTAG_C
Cuts at: 136 136
Size: 5696
NspI r_CATG'y
Cuts at: 378 1395 2186 2749 3249 4852 378
Size: 1017 791 563 500 1603 1222
Fragments arranged by size:
1603 1222 1017 791 563 500
PciI A'CATG_T
Cuts at: 374 4848 374
Size: 4474 1222
PflMI CCAn_nnn'nTGG
Cuts at: 651 651
Size: 5696
PfoI T'CCnGG_A
Cuts at: 2030 2030
Size: 5696
PmeI GTTT'AAAC
Cuts at: 2695 2695
Size: 5696
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1579 1611 4142 4174 1579
Size: 32 2531 32 3101
Fragments arranged by size:
3101 2531 32 32
PpuMI rG'GwC_Cy
Cuts at: 121 2034 121
Size: 1913 3783
PspXI vC'TCGA_Gb
Cuts at: 1099 1099
Size: 5696
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2438 2470 2438
Size: 32 5664
PstI C_TGCA'G
Cuts at: 2442 2743 3428 2442
Size: 301 685 4710
Fragments arranged by size:
4710 685 301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5696
PvuII CAG'CTG
Cuts at: 1503 2469 3121 3481 1503
Size: 966 652 360 3718
Fragments arranged by size:
3718 966 652 360
RsrII CG'GwC_CG
Cuts at: 3891 3891
Size: 5696
SacI G_AGCT'C
Cuts at: 1307 2009 2710 1307
Size: 702 701 4293
Fragments arranged by size:
4293 702 701
SalI G'TCGA_C
Cuts at: 2733 2733
Size: 5696
SanDI GG'GwC_CC
Cuts at: 121 121
Size: 5696
SapI GCTCTTCn'nnn_
Cuts at: 1222 3719 3929 1222
Size: 2497 210 2989
Fragments arranged by size:
2989 2497 210
SbfI CC_TGCA'GG
Cuts at: 2743 2743
Size: 5696
ScaI AGT'ACT
Cuts at: 5213 5213
Size: 5696
SexAI A'CCwGG_T
Cuts at: 2408 2408
Size: 5696
SfcI C'TryA_G
Cuts at: 33 1178 1627 2013 2315 2364 2438 2739 3424
Size: 1145 449 386 302 49 74 301 685
Cuts at: 3424 4392 4583 33
Size: 968 191 1146
Fragments arranged by size:
1146 1145 968 685 449 386 302 301
191 74 49
SfoI GGC'GCC
Cuts at: 3128 3376 3128
Size: 248 5448
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5696
SmaI CCC'GGG
Cuts at: 2718 2718
Size: 5696
SmlI C'TyrA_G
Cuts at: 1099 2851 3766 4480 4742 1099
Size: 1752 915 714 262 2053
Fragments arranged by size:
2053 1752 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5696
SphI G_CATG'C
Cuts at: 2749 3249 2749
Size: 500 5196
SspI AAT'ATT
Cuts at: 335 1009 335
Size: 674 5022
StuI AGG'CCT
Cuts at: 2635 2635
Size: 5696
StyI C'CwwG_G
Cuts at: 107 168 2636 2830 3184 107
Size: 61 2468 194 354 2619
Fragments arranged by size:
2619 2468 354 194 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 468 1234 1386 3279 3412 3871 4080 468
Size: 766 152 1893 133 459 209 2084
Fragments arranged by size:
2084 1893 766 459 209 152 133
TatI w'GTAC_w
Cuts at: 377 552 994 5211 377
Size: 175 442 4217 862
Fragments arranged by size:
4217 862 442 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 372 404 571 603 1706 1738 5213 5245 372
Size: 32 167 32 1103 32 3475 32 823
Fragments arranged by size:
3475 1103 823 167 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3493 3493
Size: 5696
XbaI T'CTAG_A
Cuts at: 2727 2727
Size: 5696
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1920 3191 114
Size: 1806 1271 2619
Fragments arranged by size:
2619 1806 1271
XhoI C'TCGA_G
Cuts at: 1099 1099
Size: 5696
XmaI C'CCGG_G
Cuts at: 2716 2716
Size: 5696
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AvrII BbvCI BcgI BfrBI BmgBI BsiWI BsmBI BspEI
BssHII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI NdeI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI SacII SfiI SgrAI SnaBI SrfI
SwaI XmnI ZraI