Restriction map for
pF1KA1582
(Circular) (Six-base) MAPSORT of: pF1KA1582.seq Check: 5517 from: 1 to: 8183
pF1KA1582 8183 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 779 1656 779
Size: 877 7306
AatII G_ACGT'C
Cuts at: 2899 4334 2899
Size: 1435 6748
AccI GT'mk_AC
Cuts at: 5221 5221
Size: 8183
Acc65I G'GTAC_C
Cuts at: 2196 2287 5199 2196
Size: 91 2912 5180
Fragments arranged by size:
5180 2912 91
AclI AA'CG_TT
Cuts at: 3594 8023 3594
Size: 4429 3754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 1604 3244 6008 6440 6793 7455 851
Size: 753 1640 2764 432 353 662 1579
Fragments arranged by size:
2764 1640 1579 753 662 432 353
AfeI AGC'GCT
Cuts at: 3027 3027
Size: 8183
AflIII A'CryG_T
Cuts at: 2336 2804 4817 5535 7335 7616 2336
Size: 468 2013 718 1800 281 2903
Fragments arranged by size:
2903 2013 1800 718 468 281
AgeI A'CCGG_T
Cuts at: 2199 3952 2199
Size: 1753 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 985 1496 3686 3809 5059 985
Size: 511 2190 123 1250 4109
Fragments arranged by size:
4109 2190 1250 511 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1576 1608 1576
Size: 32 8151
AlwNI CAG_nnn'CTG
Cuts at: 1627 1990 2027 2852 3264 3735 4649 4902 5488
Size: 363 37 825 412 471 914 253 586
Cuts at: 5488 6926 1627
Size: 1438 2884
Fragments arranged by size:
2884 1438 914 825 586 471 412 363
253 37
ApaI G_GGCC'C
Cuts at: 5089 5089
Size: 8183
ApaLI G'TGCA_C
Cuts at: 3245 7021 3245
Size: 3776 4407
ApoI r'AATT_y
Cuts at: 1489 3303 4270 5187 7536 7792 1489
Size: 1814 967 917 2349 256 1880
Fragments arranged by size:
2349 1880 1814 967 917 256
AseI AT'TA_AT
Cuts at: 20 395 3536 20
Size: 375 3141 4667
Fragments arranged by size:
4667 3141 375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8183
AvaI C'yCGr_G
Cuts at: 1707 2964 4605 5203 1707
Size: 1257 1641 598 4687
Fragments arranged by size:
4687 1641 1257 598
AvrII C'CTAG_G
Cuts at: 1504 1504
Size: 8183
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2757 2790 2757
Size: 33 8150
BamHI G'GATC_C
Cuts at: 955 3643 5208 955
Size: 2688 1565 3930
Fragments arranged by size:
3930 2688 1565
BanI G'GyrC_C
Cuts at: 172 2196 2287 4497 4570 5058 5199 5613 5861
Size: 2024 91 2210 73 488 141 414 248
Cuts at: 5861 5896 172
Size: 35 2459
Fragments arranged by size:
2459 2210 2024 488 414 248 141 91
73 35
BanII G_rGCy'C
Cuts at: 3191 5089 5197 6227 3191
Size: 1898 108 1030 5147
Fragments arranged by size:
5147 1898 1030 108
BbeI G_GCGC'C
Cuts at: 5617 5865 5617
Size: 248 7935
BbsI GAAGACnn'nnnn_
Cuts at: 160 2899 3128 3869 160
Size: 2739 229 741 4474
Fragments arranged by size:
4474 2739 741 229
BbvCI CC'TCA_GC
Cuts at: 2292 2700 2978 2292
Size: 408 278 7497
Fragments arranged by size:
7497 408 278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1895 1929 3785 3819 4635 4669 1895
Size: 34 1856 34 816 34 5409
Fragments arranged by size:
5409 1856 816 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6081 7137 8183 6081
Size: 1056 1046 6081
Fragments arranged by size:
6081 1056 1046
BclI T'GATC_A
Cuts at: 2936 3012 5703 2936
Size: 76 2691 5416
Fragments arranged by size:
5416 2691 76
BglI GCCn_nnn'nGGC
Cuts at: 1270 4583 5024 5084 1270
Size: 3313 441 60 4369
Fragments arranged by size:
4369 3313 441 60
BglII A'GATC_T
Cuts at: 2011 5698 7735 2011
Size: 3687 2037 2459
Fragments arranged by size:
3687 2459 2037
BlpI GC'TnA_GC
Cuts at: 4954 5161 5295 4954
Size: 207 134 7842
Fragments arranged by size:
7842 207 134
Bme1580I G_kGCm'C
Cuts at: 126 216 1400 3249 4502 5063 5089 5808 5901
Size: 90 1184 1849 1253 561 26 719 93
Cuts at: 5901 7025 126
Size: 1124 1284
Fragments arranged by size:
1849 1284 1253 1184 1124 719 561 93
90 26
BmgBI CAC'GTC
Cuts at: 2805 3051 4439 2805
Size: 246 1388 6549
Fragments arranged by size:
6549 1388 246
BmrI ACTGGGnnnn_n'
Cuts at: 738 1349 1860 2079 2115 2151 3248 3419 3975
Size: 611 511 219 36 36 1097 171 556
Cuts at: 3975 3981 3996 4031 4364 5058 5395 5476 5811
Size: 6 15 35 333 694 337 81 335
Cuts at: 5811 7955 738
Size: 2144 966
Fragments arranged by size:
2144 1097 966 694 611 556 511 337
335 333 219 171 81 36 36 35
15 6
BmtI G_CTAG'C
Cuts at: 2114 2114
Size: 8183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 6550 4547
Size: 2003 6180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 1902 4075 4094 4109 4517 4638 5055 264
Size: 1638 2173 19 15 408 121 417 3392
Fragments arranged by size:
3392 2173 1638 417 408 121 19 15
Bpu10I CC'TnA_GC
Cuts at: 2292 2700 2978 2292
Size: 408 278 7497
Fragments arranged by size:
7497 408 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 794 3422 4387 4736 5359 6238 6952 7250 794
Size: 2628 965 349 623 879 714 298 1727
Fragments arranged by size:
2628 1727 965 879 714 623 349 298
BsaI GGTCTCn'nnnn_
Cuts at: 3511 7887 3511
Size: 4376 3807
BsaAI yAC'GTr
Cuts at: 4213 5536 4213
Size: 1323 6860
BsaBI GATnn'nnATC
Cuts at: 6 5697 7734 6
Size: 5691 2037 455
Fragments arranged by size:
5691 2037 455
BsaHI Gr'CG_yC
Cuts at: 2437 2896 4331 5614 5862 6564 8060 2437
Size: 459 1435 1283 248 702 1496 2560
Fragments arranged by size:
2560 1496 1435 1283 702 459 248
BsaWI w'CCGG_w
Cuts at: 2199 3640 3952 4769 5372 5893 6571 6982 7129
Size: 1441 312 817 603 521 678 411 147
Cuts at: 7129 7403 2199
Size: 274 2979
Fragments arranged by size:
2979 1441 817 678 603 521 411 312
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 908 938 1240 1270 1792 1822 3879 3909 4003
Size: 30 302 30 522 30 2057 30 94
Cuts at: 4003 4033 908
Size: 30 5058
Fragments arranged by size:
5058 2057 522 302 94 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2162 2829 2995 4390 4548 4593 4801 2162
Size: 667 166 1395 158 45 208 5544
Fragments arranged by size:
5544 1395 667 208 166 158 45
BseYI C'CCAG_C
Cuts at: 365 423 1099 2027 2234 2513 2620 2741 3066
Size: 58 676 928 207 279 107 121 325
Cuts at: 3066 3285 3419 3626 5092 5608 6366 7031 7439
Size: 219 134 207 1466 516 758 665 408
Cuts at: 7439 365
Size: 1109
Fragments arranged by size:
1466 1109 928 758 676 665 516 408
325 279 219 207 207 134 121 107
58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1571 1907 3421 3500 3769 4499 4798 1571
Size: 336 1514 79 269 730 299 4956
Fragments arranged by size:
4956 1514 730 336 299 269 79
BsiEI CG_ry'CG
Cuts at: 104 5725 5771 7001 104
Size: 5621 46 1230 1286
Fragments arranged by size:
5621 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 586 3249 3438 4624 5197 5975 6165 7025 586
Size: 2663 189 1186 573 778 190 860 1744
Fragments arranged by size:
2663 1744 1186 860 778 573 190 189
BsmI GAATG_Cn'
Cuts at: 1155 1155
Size: 8183
BsmBI CGTCTCn'nnnn_
Cuts at: 2428 2428
Size: 8183
Bsp1286I G_dGCh'C
Cuts at: 126 216 586 1400 3191 3249 3438 4502 4624
Size: 90 370 814 1791 58 189 1064 122
Cuts at: 4624 5063 5089 5197 5808 5901 5975 6165 6227
Size: 439 26 108 611 93 74 190 62
Cuts at: 6227 7025 126
Size: 798 1284
Fragments arranged by size:
1791 1284 1064 814 798 611 439 370
190 189 122 108 93 90 74 62
58 26
BspEI T'CCGG_A
Cuts at: 3640 3640
Size: 8183
BspHI T'CATG_A
Cuts at: 1598 2924 4482 6615 1598
Size: 1326 1558 2133 3166
Fragments arranged by size:
3166 2133 1558 1326
BspMI ACCTGCnnnn'nnnn_
Cuts at: 779 1656 3863 5165 5233 5447 5749 6130 779
Size: 877 2207 1302 68 214 302 381 2832
Fragments arranged by size:
2832 2207 1302 877 381 302 214 68
BsrBI CCG'CTC
Cuts at: 50 6475 6529 8013 8178 50
Size: 6425 54 1484 165 55
Fragments arranged by size:
6425 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 710 4764 6095 710
Size: 4054 1331 2798
Fragments arranged by size:
4054 2798 1331
BsrFI r'CCGG_y
Cuts at: 2193 2199 2661 2930 3952 4142 6181 6362 2193
Size: 6 462 269 1022 190 2039 181 4014
Fragments arranged by size:
4014 2039 1022 462 269 190 181 6
BssHII G'CGCG_C
Cuts at: 3673 3673
Size: 8183
BssSI C'ACGA_G
Cuts at: 2007 2409 3178 3739 4521 6454 7162 2007
Size: 402 769 561 782 1933 708 3028
Fragments arranged by size:
3028 1933 782 769 708 561 402
BstAPI GCAn_nnn'nTGC
Cuts at: 940 2692 5560 940
Size: 1752 2868 3563
Fragments arranged by size:
3563 2868 1752
BstBI TT'CG_AA
Cuts at: 4662 6544 4662
Size: 1882 6301
BstEII G'GTnAC_C
Cuts at: 868 868
Size: 8183
BstXI CCAn_nnnn'nTGG
Cuts at: 431 4023 4684 431
Size: 3592 661 3930
Fragments arranged by size:
3930 3592 661
BstYI r'GATC_y
Cuts at: 1 955 1954 2011 3643 3701 5208 5675 5698
Size: 954 999 57 1632 58 1507 467 23
Cuts at: 5698 6033 6279 6585 6597 6683 7735 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1632 1507 1052 999 954 467 449 335
306 246 86 58 57 23 12
Bsu36I CC'TnA_GG
Cuts at: 3461 4632 3461
Size: 1171 7012
BtgI C'CryG_G
Cuts at: 107 458 3140 3809 4022 4288 4948 4974 107
Size: 351 2682 669 213 266 660 26 3316
Fragments arranged by size:
3316 2682 669 660 351 266 213 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6133 6314 6477 6133
Size: 181 163 7839
Fragments arranged by size:
7839 181 163
BtsI GCAGTG_nn'
Cuts at: 297 831 969 1056 1478 2226 2631 2754 4350
Size: 534 138 87 422 748 405 123 1596
Cuts at: 4350 4693 5043 5426 297
Size: 343 350 383 3054
Fragments arranged by size:
3054 1596 748 534 422 405 383 350
343 138 123 87
ClaI AT'CG_AT
Cuts at: 2472 2472
Size: 8183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3141 3176 3141
Size: 35 8148
DraI TTT'AAA
Cuts at: 2084 5182 2084
Size: 3098 5085
DraIII CAC_nnn'GTG
Cuts at: 3244 3244
Size: 8183
DrdI GACnn_nn'nnGTC
Cuts at: 4330 5889 7233 4330
Size: 1559 1344 5280
Fragments arranged by size:
5280 1559 1344
EaeI y'GGCC_r
Cuts at: 251 1770 3092 3806 4140 4847 4998 5768 5942
Size: 1519 1322 714 334 707 151 770 174
Cuts at: 5942 6333 6360 7705 251
Size: 391 27 1345 729
Fragments arranged by size:
1519 1345 1322 770 729 714 707 391
334 174 151 27
EagI C'GGCC_G
Cuts at: 5768 5768
Size: 8183
EarI CTCTTCn'nnn_
Cuts at: 687 1623 2670 2986 3179 3420 6206 6416 687
Size: 936 1047 316 193 241 2786 210 2454
Fragments arranged by size:
2786 2454 1047 936 316 241 210 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6391 7133 7279 7993 6391
Size: 742 146 714 6581
Fragments arranged by size:
6581 742 714 146
EcoICRI GAG'CTC
Cuts at: 5195 5195
Size: 8183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 851 1604 1902 3244 4075 4094 4109 4517
Size: 587 753 298 1342 831 19 15 408
Cuts at: 4517 4638 5055 6008 6440 6793 7455 264
Size: 121 417 953 432 353 662 992
Fragments arranged by size:
1342 992 953 831 753 662 587 432
417 408 353 298 121 19 15
EcoNI CCTnn'n_nnAGG
Cuts at: 878 1041 878
Size: 163 8020
EcoO109I rG'GnC_Cy
Cuts at: 1009 2304 3261 3732 3972 5085 5154 5322 1009
Size: 1295 957 471 240 1113 69 168 3870
Fragments arranged by size:
3870 1295 1113 957 471 240 168 69
EcoRI G'AATT_C
Cuts at: 3303 4270 5187 3303
Size: 967 917 6299
Fragments arranged by size:
6299 967 917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3493 6202 3493
Size: 2709 5474
FspI TGC'GCA
Cuts at: 5964 5964
Size: 8183
HaeII r_GCGC'y
Cuts at: 1699 3029 3720 4903 5099 5617 5865 7095 7866
Size: 1330 691 1183 196 518 248 1230 771
Cuts at: 7866 1699
Size: 2016
Fragments arranged by size:
2016 1330 1230 1183 771 691 518 248
196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1792 1824 1900 1932 2275 2307 3315 3347 3859
Size: 32 76 32 343 32 1008 32 512
Cuts at: 3859 3891 4222 4254 4417 4449 6025 6055 6057
Size: 32 331 32 163 32 1576 30 2
Cuts at: 6057 6087 1792
Size: 30 3888
Fragments arranged by size:
3888 1576 1008 512 343 331 163 76
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1638 3936 4389 4541 5222 1638
Size: 2298 453 152 681 4599
Fragments arranged by size:
4599 2298 681 453 152
HpaI GTT'AAC
Cuts at: 4541 4541
Size: 8183
KasI G'GCGC_C
Cuts at: 5613 5861 5613
Size: 248 7935
KpnI G_GTAC'C
Cuts at: 2200 2291 5203 2200
Size: 91 2912 5180
Fragments arranged by size:
5180 2912 91
MluI A'CGCG_T
Cuts at: 7616 7616
Size: 8183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 401 1184 1975 6942 7126 401
Size: 783 791 4967 184 1458
Fragments arranged by size:
4967 1458 791 783 184
MscI TGG'CCA
Cuts at: 1772 5000 5944 1772
Size: 3228 944 4011
Fragments arranged by size:
4011 3228 944
MslI CAynn'nnrTG
Cuts at: 2953 3946 6172 6299 2953
Size: 993 2226 127 4837
Fragments arranged by size:
4837 2226 993 127
MspA1I CmG'CkG
Cuts at: 427 1654 2466 2633 2977 3576 3582 3723 4649
Size: 1227 812 167 344 599 6 141 926
Cuts at: 4649 4919 4950 4963 5165 5294 5608 5968 6752
Size: 270 31 13 202 129 314 360 784
Cuts at: 6752 6997 427
Size: 245 1613
Fragments arranged by size:
1613 1227 926 812 784 599 360 344
314 270 245 202 167 141 129 31
13 6
NaeI GCC'GGC
Cuts at: 2932 6364 2932
Size: 3432 4751
NarI GG'CG_CC
Cuts at: 5614 5862 5614
Size: 248 7935
NcoI C'CATG_G
Cuts at: 107 3809 4022 4288 4974 107
Size: 3702 213 266 686 3316
Fragments arranged by size:
3702 3316 686 266 213
NdeI CA'TA_TG
Cuts at: 3548 3548
Size: 8183
NgoMIV G'CCGG_C
Cuts at: 2930 6362 2930
Size: 3432 4751
NheI G'CTAG_C
Cuts at: 2110 2110
Size: 8183
NspI r_CATG'y
Cuts at: 2340 3294 4821 5236 5736 7339 2340
Size: 954 1527 415 500 1603 3184
Fragments arranged by size:
3184 1603 1527 954 500 415
PasI CC'CwG_GG
Cuts at: 126 3264 126
Size: 3138 5045
PciI A'CATG_T
Cuts at: 2336 4817 7335 2336
Size: 2481 2518 3184
Fragments arranged by size:
3184 2518 2481
PflMI CCAn_nnn'nTGG
Cuts at: 260 2783 4649 4980 260
Size: 2523 1866 331 3463
Fragments arranged by size:
3463 2523 1866 331
PfoI T'CCnGG_A
Cuts at: 1023 4278 4379 1023
Size: 3255 101 4827
Fragments arranged by size:
4827 3255 101
PmeI GTTT'AAAC
Cuts at: 5182 5182
Size: 8183
PmlI CAC'GTG
Cuts at: 4213 4213
Size: 8183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6629 6661 6629
Size: 32 8151
PpuMI rG'GwC_Cy
Cuts at: 1009 2304 3972 5154 1009
Size: 1295 1668 1182 4038
Fragments arranged by size:
4038 1668 1295 1182
PspOMI G'GGCC_C
Cuts at: 5085 5085
Size: 8183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1296 1328 1296
Size: 32 8151
PstI C_TGCA'G
Cuts at: 2848 3581 3695 3798 3860 4304 5230 5915 2848
Size: 733 114 103 62 444 926 685 5116
Fragments arranged by size:
5116 926 733 685 444 114 103 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8183
PvuII CAG'CTG
Cuts at: 427 2466 2977 3576 3582 3723 4649 4919 5608
Size: 2039 511 599 6 141 926 270 689
Cuts at: 5608 5968 427
Size: 360 2642
Fragments arranged by size:
2642 2039 926 689 599 511 360 270
141 6
RsrII CG'GwC_CG
Cuts at: 6378 6378
Size: 8183
SacI G_AGCT'C
Cuts at: 5197 5197
Size: 8183
SacII CC_GC'GG
Cuts at: 4951 4951
Size: 8183
SalI G'TCGA_C
Cuts at: 5220 5220
Size: 8183
SanDI GG'GwC_CC
Cuts at: 2304 2304
Size: 8183
SapI GCTCTTCn'nnn_
Cuts at: 2670 3179 6206 6416 2670
Size: 509 3027 210 4437
Fragments arranged by size:
4437 3027 509 210
SbfI CC_TGCA'GG
Cuts at: 5230 5230
Size: 8183
ScaI AGT'ACT
Cuts at: 1749 4512 5068 7700 1749
Size: 2763 556 2632 2232
Fragments arranged by size:
2763 2632 2232 556
SfcI C'TryA_G
Cuts at: 33 113 1286 2844 3577 3691 3794 3856 4300
Size: 80 1173 1558 733 114 103 62 444
Cuts at: 4300 5226 5911 6879 7070 33
Size: 926 685 968 191 1146
Fragments arranged by size:
1558 1173 1146 968 926 733 685 444
191 114 103 80 62
SfoI GGC'GCC
Cuts at: 5615 5863 5615
Size: 248 7935
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8183
SgrAI Cr'CCGG_yG
Cuts at: 2661 2661
Size: 8183
SmaI CCC'GGG
Cuts at: 1709 5205 1709
Size: 3496 4687
SmlI C'TyrA_G
Cuts at: 809 3437 4402 4751 5338 6253 6967 7229 809
Size: 2628 965 349 587 915 714 262 1763
Fragments arranged by size:
2628 1763 965 915 714 587 349 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8183
SphI G_CATG'C
Cuts at: 3294 5236 5736 3294
Size: 1942 500 5741
Fragments arranged by size:
5741 1942 500
SspI AAT'ATT
Cuts at: 4308 4308
Size: 8183
StuI AGG'CCT
Cuts at: 779 1540 2917 2986 779
Size: 761 1377 69 5976
Fragments arranged by size:
5976 1377 761 69
StyI C'CwwG_G
Cuts at: 107 216 1392 1411 1504 1923 3809 4022 4288
Size: 109 1176 19 93 419 1886 213 266
Cuts at: 4288 4781 4796 4890 4974 5317 5671 107
Size: 493 15 94 84 343 354 2619
Fragments arranged by size:
2619 1886 1176 493 419 354 343 266
213 109 94 93 84 19 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 702 1619 1827 2634 3091 3256 3845 3897 4533
Size: 917 208 807 457 165 589 52 636
Cuts at: 4533 4923 5011 5766 5899 6358 6567 702
Size: 390 88 755 133 459 209 2318
Fragments arranged by size:
2318 917 807 755 636 589 459 457
390 209 208 165 133 88 52
TatI w'GTAC_w
Cuts at: 438 1606 1747 4510 5066 7698 438
Size: 1168 141 2763 556 2632 923
Fragments arranged by size:
2763 2632 1168 923 556 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 908 940 1240 1272 3879 3911 4003 4035 7700
Size: 32 300 32 2607 32 92 32 3665
Cuts at: 7700 7732 908
Size: 32 1359
Fragments arranged by size:
3665 2607 1359 300 92 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4440 5980 4440
Size: 1540 6643
XbaI T'CTAG_A
Cuts at: 3310 5214 3310
Size: 1904 6279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 1780 2066 3261 5678 288
Size: 1492 286 1195 2417 2793
Fragments arranged by size:
2793 2417 1492 1195 286
XmaI C'CCGG_G
Cuts at: 1707 5203 1707
Size: 3496 4687
XmnI GAAnn'nnTTC
Cuts at: 4879 4879
Size: 8183
ZraI GAC'GTC
Cuts at: 2897 4332 2897
Size: 1435 6748
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI BfrBI BsiWI BsrGI BstZ17I
EcoRV FseI FspAI HindIII MfeI NotI NruI NsiI
PacI PshAI PsiI PspXI SexAI SfiI SnaBI SrfI
SwaI XhoI