Restriction map for
pF1KA1590
(Circular) (Six-base) MAPSORT of: pF1KA1590.seq Check: 5689 from: 1 to: 7289
pF1KA1590 7289 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1125 1125
Size: 7289
AatII G_ACGT'C
Cuts at: 764 764
Size: 7289
AccI GT'mk_AC
Cuts at: 4327 4327
Size: 7289
Acc65I G'GTAC_C
Cuts at: 4305 4305
Size: 7289
AclI AA'CG_TT
Cuts at: 7129 7129
Size: 7289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 566 1315 1806 3481 4094 4129 5114 5546
Size: 86 749 491 1675 613 35 985 432
Cuts at: 5546 6293 6561 480
Size: 747 268 1208
Fragments arranged by size:
1675 1208 985 749 747 613 491 432
268 86 35
AflIII A'CryG_T
Cuts at: 576 4641 5745 6722 576
Size: 4065 1104 977 1143
Fragments arranged by size:
4065 1143 1104 977
AgeI A'CCGG_T
Cuts at: 5677 5677
Size: 7289
AleI CACnn'nnGTG
Cuts at: 2767 3862 2767
Size: 1095 6194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3230 3230
Size: 7289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 2669 2701 160
Size: 32 2477 32 4748
Fragments arranged by size:
4748 2477 32 32
AlwNI CAG_nnn'CTG
Cuts at: 748 2442 3327 3643 3849 4594 6161 748
Size: 1694 885 316 206 745 1567 1876
Fragments arranged by size:
1876 1694 1567 885 745 316 206
ApaI G_GGCC'C
Cuts at: 3132 3132
Size: 7289
ApaLI G'TGCA_C
Cuts at: 3995 6059 3995
Size: 2064 5225
ApoI r'AATT_y
Cuts at: 450 563 811 1897 2140 2587 2894 3028 3763
Size: 113 248 1086 243 447 307 134 735
Cuts at: 3763 4033 4293 6642 6898 450
Size: 270 260 2349 256 841
Fragments arranged by size:
2349 1086 841 735 447 307 270 260
256 248 243 134 113
AseI AT'TA_AT
Cuts at: 20 1199 20
Size: 1179 6110
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7289
AvaI C'yCGr_G
Cuts at: 4309 4309
Size: 7289
BamHI G'GATC_C
Cuts at: 4314 4314
Size: 7289
BanI G'GyrC_C
Cuts at: 3577 4305 4719 4967 5002 3577
Size: 728 414 248 35 5864
Fragments arranged by size:
5864 728 414 248 35
BanII G_rGCy'C
Cuts at: 3132 3254 4303 5333 3132
Size: 122 1049 1030 5088
Fragments arranged by size:
5088 1049 1030 122
BbeI G_GCGC'C
Cuts at: 4723 4971 4723
Size: 248 7041
BbsI GAAGACnn'nnnn_
Cuts at: 3950 4080 3950
Size: 130 7159
BbvCI CC'TCA_GC
Cuts at: 2158 2158
Size: 7289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 345 379 345
Size: 34 7255
BciVI GTATCCnnnnn_n'
Cuts at: 5187 5948 7289 5187
Size: 761 1341 5187
Fragments arranged by size:
5187 1341 761
BclI T'GATC_A
Cuts at: 4010 4809 4010
Size: 799 6490
BglII A'GATC_T
Cuts at: 585 3684 4804 6841 585
Size: 3099 1120 2037 1033
Fragments arranged by size:
3099 2037 1120 1033
BlpI GC'TnA_GC
Cuts at: 3272 4401 3272
Size: 1129 6160
Bme1580I G_kGCm'C
Cuts at: 3132 3187 3999 4914 5007 6063 3132
Size: 55 812 915 93 1056 4358
Fragments arranged by size:
4358 1056 915 812 93 55
BmgBI CAC'GTC
Cuts at: 3049 3049
Size: 7289
BmrI ACTGGGnnnn_n'
Cuts at: 251 758 1662 4501 4582 4917 7061 251
Size: 507 904 2839 81 335 2144 479
Fragments arranged by size:
2839 2144 904 507 479 335 81
BmtI G_CTAG'C
Cuts at: 3263 3263
Size: 7289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2929 5656 2929
Size: 2727 4562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 2017 2038 3142 3163 3238 3286 3307 3661
Size: 1541 21 1104 21 75 48 21 354
Cuts at: 3661 476
Size: 4104
Fragments arranged by size:
4104 1541 1104 354 75 48 21 21
21
Bpu10I CC'TnA_GC
Cuts at: 972 1786 2158 4136 972
Size: 814 372 1978 4125
Fragments arranged by size:
4125 1978 814 372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 2759 4226 4465 5344 5836 6134 1256
Size: 1503 1467 239 879 492 298 2411
Fragments arranged by size:
2411 1503 1467 879 492 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 779 2014 2200 6993 779
Size: 1235 186 4793 1075
Fragments arranged by size:
4793 1235 1075 186
BsaAI yAC'GTr
Cuts at: 4642 4642
Size: 7289
BsaBI GATnn'nnATC
Cuts at: 6 1480 3506 4803 6840 6
Size: 1474 2026 1297 2037 455
Fragments arranged by size:
2037 2026 1474 1297 455
BsaHI Gr'CG_yC
Cuts at: 761 3492 4720 4968 5670 7166 761
Size: 2731 1228 248 702 1496 884
Fragments arranged by size:
2731 1496 1228 884 702 248
BsaWI w'CCGG_w
Cuts at: 731 892 4478 4999 5677 5951 6098 6509 731
Size: 161 3586 521 678 274 147 411 1511
Fragments arranged by size:
3586 1511 678 521 411 274 161 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 160 190 2446 2476 2825 2855 3737 3767 160
Size: 30 2256 30 349 30 882 30 3682
Fragments arranged by size:
3682 2256 882 349 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2086 2164 2377 2488 2569 3162 3977 2086
Size: 78 213 111 81 593 815 5398
Fragments arranged by size:
5398 815 593 213 111 81 78
BseYI C'CCAG_C
Cuts at: 2024 4714 5472 6049 6545 2024
Size: 2690 758 577 496 2768
Fragments arranged by size:
2768 2690 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 2068 902
Size: 1166 6123
BsiEI CG_ry'CG
Cuts at: 104 4831 4877 6085 104
Size: 4727 46 1208 1308
Fragments arranged by size:
4727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1675 3999 4142 4303 5081 5271 6063 1675
Size: 2324 143 161 778 190 792 2901
Fragments arranged by size:
2901 2324 792 778 190 161 143
BsmI GAATG_Cn'
Cuts at: 2495 3965 2495
Size: 1470 5819
BsmBI CGTCTCn'nnnn_
Cuts at: 1547 1547
Size: 7289
Bsp1286I G_dGCh'C
Cuts at: 1675 3132 3187 3254 3999 4142 4303 4914 5007
Size: 1457 55 67 745 143 161 611 93
Cuts at: 5007 5081 5271 5333 6063 1675
Size: 74 190 62 730 2901
Fragments arranged by size:
2901 1457 745 730 611 190 161 143
93 74 67 62 55
BspEI T'CCGG_A
Cuts at: 6509 6509
Size: 7289
BspHI T'CATG_A
Cuts at: 1097 6465 1097
Size: 5368 1921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 761 1125 2370 4339 4553 4855 5236 761
Size: 364 1245 1969 214 302 381 2814
Fragments arranged by size:
2814 1969 1245 381 364 302 214
BsrBI CCG'CTC
Cuts at: 50 2001 3126 5581 5635 7119 7284 50
Size: 1951 1125 2455 54 1484 165 55
Fragments arranged by size:
2455 1951 1484 1125 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1611 3041 3926 5201 1611
Size: 1430 885 1275 3699
Fragments arranged by size:
3699 1430 1275 885
BsrFI r'CCGG_y
Cuts at: 5287 5468 5677 5287
Size: 181 209 6899
Fragments arranged by size:
6899 209 181
BssSI C'ACGA_G
Cuts at: 2389 2528 5560 5918 2389
Size: 139 3032 358 3760
Fragments arranged by size:
3760 3032 358 139
BstAPI GCAn_nnn'nTGC
Cuts at: 2385 4666 2385
Size: 2281 5008
BstBI TT'CG_AA
Cuts at: 540 2585 5650 540
Size: 2045 3065 2179
Fragments arranged by size:
3065 2179 2045
BstYI r'GATC_y
Cuts at: 1 421 479 585 848 2684 3684 4314 4781
Size: 420 58 106 263 1836 1000 630 467
Cuts at: 4781 4804 5139 5385 6397 6483 6495 6841 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1836 1012 1000 630 467 449 420 346
335 263 246 106 86 58 23 12
Bsu36I CC'TnA_GG
Cuts at: 1426 4214 1426
Size: 2788 4501
BtgI C'CryG_G
Cuts at: 107 1565 107
Size: 1458 5831
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5239 5420 5583 5239
Size: 181 163 6945
Fragments arranged by size:
6945 181 163
BtsI GCAGTG_nn'
Cuts at: 3032 4532 3032
Size: 1500 5789
ClaI AT'CG_AT
Cuts at: 1484 1484
Size: 7289
DraI TTT'AAA
Cuts at: 1526 2699 4288 1526
Size: 1173 1589 4527
Fragments arranged by size:
4527 1589 1173
DrdI GACnn_nn'nnGTC
Cuts at: 497 4995 5853 497
Size: 4498 858 1933
Fragments arranged by size:
4498 1933 858
EaeI y'GGCC_r
Cuts at: 125 3134 3885 4874 5048 5439 5466 6811 125
Size: 3009 751 989 174 391 27 1345 603
Fragments arranged by size:
3009 1345 989 751 603 391 174 27
EagI C'GGCC_G
Cuts at: 4874 4874
Size: 7289
EarI CTCTTCn'nnn_
Cuts at: 249 499 1157 1789 1795 2141 2185 2198 2399
Size: 250 658 632 6 346 44 13 201
Cuts at: 2399 2465 2473 3191 5312 5522 249
Size: 66 8 718 2121 210 2016
Fragments arranged by size:
2121 2016 718 658 632 346 250 210
201 66 44 13 8 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 612 5497 5807 5953 7099 612
Size: 4885 310 146 1146 802
Fragments arranged by size:
4885 1146 802 310 146
EcoICRI GAG'CTC
Cuts at: 4301 4301
Size: 7289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 480 566 1315 1806 2017 2038 3142 3163
Size: 4 86 749 491 211 21 1104 21
Cuts at: 3163 3238 3286 3307 3481 3661 4094 4129 5114
Size: 75 48 21 174 180 433 35 985
Cuts at: 5114 5546 6293 6561 476
Size: 432 747 268 1204
Fragments arranged by size:
1204 1104 985 749 747 491 433 432
268 211 180 174 86 75 48 35
21 21 21 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3289 3289
Size: 7289
EcoO109I rG'GnC_Cy
Cuts at: 649 1664 2484 2631 2908 3129 3377 4428 649
Size: 1015 820 147 277 221 248 1051 3510
Fragments arranged by size:
3510 1051 1015 820 277 248 221 147
EcoRI G'AATT_C
Cuts at: 2587 3028 4033 4293 2587
Size: 441 1005 260 5583
Fragments arranged by size:
5583 1005 441 260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 870 1090 2989 5308 870
Size: 220 1899 2319 2851
Fragments arranged by size:
2851 2319 1899 220
FspI TGC'GCA
Cuts at: 5070 5070
Size: 7289
HaeII r_GCGC'y
Cuts at: 3121 4723 4971 5993 6972 3121
Size: 1602 248 1022 979 3438
Fragments arranged by size:
3438 1602 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 652 684 1629 1661 1849 1881 2704 2736 5131
Size: 32 945 32 188 32 823 32 2395
Cuts at: 5131 5161 5163 5193 652
Size: 30 2 30 2748
Fragments arranged by size:
2748 2395 945 823 188 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1052 3783 4073 4328 1052
Size: 2731 290 255 4013
Fragments arranged by size:
4013 2731 290 255
HindIII A'AGCT_T
Cuts at: 530 2335 530
Size: 1805 5484
KasI G'GCGC_C
Cuts at: 4719 4967 4719
Size: 248 7041
KpnI G_GTAC'C
Cuts at: 4309 4309
Size: 7289
MluI A'CGCG_T
Cuts at: 6722 6722
Size: 7289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 481 2259 2711 2849 5960 6144 481
Size: 1778 452 138 3111 184 1626
Fragments arranged by size:
3111 1778 1626 452 184 138
MscI TGG'CCA
Cuts at: 3136 3887 5050 3136
Size: 751 1163 5375
Fragments arranged by size:
5375 1163 751
MslI CAynn'nnrTG
Cuts at: 442 2389 2767 3862 5278 5405 442
Size: 1947 378 1095 1416 127 2326
Fragments arranged by size:
2326 1947 1416 1095 378 127
MspA1I CmG'CkG
Cuts at: 3003 3327 3652 4400 4714 5074 6087 6332 3003
Size: 324 325 748 314 360 1013 245 3960
Fragments arranged by size:
3960 1013 748 360 325 324 314 245
NaeI GCC'GGC
Cuts at: 5470 5470
Size: 7289
NarI GG'CG_CC
Cuts at: 4720 4968 4720
Size: 248 7041
NcoI C'CATG_G
Cuts at: 107 107
Size: 7289
NdeI CA'TA_TG
Cuts at: 408 408
Size: 7289
NgoMIV G'CCGG_C
Cuts at: 5468 5468
Size: 7289
NheI G'CTAG_C
Cuts at: 3259 3259
Size: 7289
NspI r_CATG'y
Cuts at: 4342 4842 5749 4342
Size: 500 907 5882
Fragments arranged by size:
5882 907 500
PasI CC'CwG_GG
Cuts at: 3180 3180
Size: 7289
PciI A'CATG_T
Cuts at: 5745 5745
Size: 7289
PflMI CCAn_nnn'nTGG
Cuts at: 521 1370 521
Size: 849 6440
PmeI GTTT'AAAC
Cuts at: 4288 4288
Size: 7289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 160 192 6428 6460 160
Size: 32 6236 32 989
Fragments arranged by size:
6236 989 32 32
PpuMI rG'GwC_Cy
Cuts at: 1664 2484 2631 3377 1664
Size: 820 147 746 5576
Fragments arranged by size:
5576 820 746 147
PsiI TTA'TAA
Cuts at: 389 569 389
Size: 180 7109
PspOMI G'GGCC_C
Cuts at: 3128 3128
Size: 7289
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 7257
PstI C_TGCA'G
Cuts at: 1872 2186 2849 3167 3532 3543 4336 5021 1872
Size: 314 663 318 365 11 793 685 4140
Fragments arranged by size:
4140 793 685 663 365 318 314 11
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7289
PvuII CAG'CTG
Cuts at: 3003 3327 4714 5074 3003
Size: 324 1387 360 5218
Fragments arranged by size:
5218 1387 360 324
RsrII CG'GwC_CG
Cuts at: 5484 5484
Size: 7289
SacI G_AGCT'C
Cuts at: 4303 4303
Size: 7289
SalI G'TCGA_C
Cuts at: 4326 4326
Size: 7289
SanDI GG'GwC_CC
Cuts at: 1664 3377 1664
Size: 1713 5576
SapI GCTCTTCn'nnn_
Cuts at: 2399 3191 5312 5522 2399
Size: 792 2121 210 4166
Fragments arranged by size:
4166 2121 792 210
SbfI CC_TGCA'GG
Cuts at: 4336 4336
Size: 7289
ScaI AGT'ACT
Cuts at: 1149 1503 6806 1149
Size: 354 5303 1632
Fragments arranged by size:
5303 1632 354
SexAI A'CCwGG_T
Cuts at: 2633 3434 2633
Size: 801 6488
SfcI C'TryA_G
Cuts at: 33 1868 2182 2833 2845 3163 3528 3539 4332
Size: 1835 314 651 12 318 365 11 793
Cuts at: 4332 5017 6010 6201 33
Size: 685 993 191 1121
Fragments arranged by size:
1835 1121 993 793 685 651 365 318
314 191 12 11
SfoI GGC'GCC
Cuts at: 4721 4969 4721
Size: 248 7041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7289
SmaI CCC'GGG
Cuts at: 4311 4311
Size: 7289
SmlI C'TyrA_G
Cuts at: 1271 2774 4241 4444 5359 5851 6113 1271
Size: 1503 1467 203 915 492 262 2447
Fragments arranged by size:
2447 1503 1467 915 492 262 203
SpeI A'CTAG_T
Cuts at: 67 4083 67
Size: 4016 3273
SphI G_CATG'C
Cuts at: 4342 4842 4342
Size: 500 6789
SspI AAT'ATT
Cuts at: 930 1401 930
Size: 471 6818
StyI C'CwwG_G
Cuts at: 107 1074 1364 3776 4423 4777 107
Size: 967 290 2412 647 354 2619
Fragments arranged by size:
2619 2412 967 647 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 105 1852 2454 3865 3937 4872 5005 5464 5673
Size: 1747 602 1411 72 935 133 459 209
Cuts at: 5673 105
Size: 1721
Fragments arranged by size:
1747 1721 1411 935 602 459 209 133
72
TatI w'GTAC_w
Cuts at: 681 1147 1501 2804 3532 6804 681
Size: 466 354 1303 728 3272 1166
Fragments arranged by size:
3272 1303 1166 728 466 354
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2446 2478 3737 3769 6806 6838 2446
Size: 32 1259 32 3037 32 2897
Fragments arranged by size:
3037 2897 1259 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5086 5086
Size: 7289
XbaI T'CTAG_A
Cuts at: 2722 4320 2722
Size: 1598 5691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2340 4784 2340
Size: 2444 4845
XmaI C'CCGG_G
Cuts at: 4309 4309
Size: 7289
XmnI GAAnn'nnTTC
Cuts at: 1328 2495 1328
Size: 1167 6122
ZraI GAC'GTC
Cuts at: 762 762
Size: 7289
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PmeI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BfrBI BglI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NotI NruI NsiI
PacI PfoI PmlI PshAI PspXI SacII SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI