Restriction map for
pF1KA1592
(Circular) (Six-base) MAPSORT of: pF1KA1592.seq Check: 5523 from: 1 to: 5006
pF1KA1592 5006 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1694 1694
Size: 5006
AccI GT'mk_AC
Cuts at: 2044 2044
Size: 5006
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 4846 4846
Size: 5006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1752 2831 3263 4010 4278 178
Size: 1574 1079 432 747 268 906
Fragments arranged by size:
1574 1079 906 747 432 268
AflIII A'CryG_T
Cuts at: 1214 2358 3462 4439 1214
Size: 1144 1104 977 1781
Fragments arranged by size:
1781 1144 1104 977
AgeI A'CCGG_T
Cuts at: 3394 3394
Size: 5006
AhdI GACnn_n'nnGTC
Cuts at: 918 918
Size: 5006
AlwNI CAG_nnn'CTG
Cuts at: 543 2311 3878 543
Size: 1768 1567 1671
Fragments arranged by size:
1768 1671 1567
ApaI G_GGCC'C
Cuts at: 138 152 138
Size: 14 4992
ApaLI G'TGCA_C
Cuts at: 126 1021 1881 3776 126
Size: 895 860 1895 1356
Fragments arranged by size:
1895 1356 895 860
ApoI r'AATT_y
Cuts at: 595 2010 4359 4615 595
Size: 1415 2349 256 986
Fragments arranged by size:
2349 1415 986 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 131 286 2026 131
Size: 155 1740 3111
Fragments arranged by size:
3111 1740 155
AvrII C'CTAG_G
Cuts at: 417 417
Size: 5006
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1399 1432 1399
Size: 33 4973
BamHI G'GATC_C
Cuts at: 2031 2031
Size: 5006
BanI G'GyrC_C
Cuts at: 337 571 766 1576 2022 2436 2684 2719 337
Size: 234 195 810 446 414 248 35 2624
Fragments arranged by size:
2624 810 446 414 248 234 195 35
BanII G_rGCy'C
Cuts at: 138 152 377 725 752 1301 2020 3050 138
Size: 14 225 348 27 549 719 1030 2094
Fragments arranged by size:
2094 1030 719 549 348 225 27 14
BbeI G_GCGC'C
Cuts at: 1580 2440 2688 1580
Size: 860 248 3898
Fragments arranged by size:
3898 860 248
BbsI GAAGACnn'nnnn_
Cuts at: 917 1023 917
Size: 106 4900
BciVI GTATCCnnnnn_n'
Cuts at: 2904 3665 5006 2904
Size: 761 1341 2904
Fragments arranged by size:
2904 1341 761
BclI T'GATC_A
Cuts at: 2526 2526
Size: 5006
BglI GCCn_nnn'nGGC
Cuts at: 147 222 381 147
Size: 75 159 4772
Fragments arranged by size:
4772 159 75
BglII A'GATC_T
Cuts at: 2521 4558 2521
Size: 2037 2969
BlpI GC'TnA_GC
Cuts at: 1659 2118 1659
Size: 459 4547
Bme1580I G_kGCm'C
Cuts at: 130 138 152 771 1025 1782 1885 2631 2724
Size: 8 14 619 254 757 103 746 93
Cuts at: 2724 3780 130
Size: 1056 1356
Fragments arranged by size:
1356 1056 757 746 619 254 103 93
14 8
BmgBI CAC'GTC
Cuts at: 1170 1170
Size: 5006
BmrI ACTGGGnnnn_n'
Cuts at: 2218 2299 2634 4778 2218
Size: 81 335 2144 2446
Fragments arranged by size:
2446 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1980 3373 1980
Size: 1393 3613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1081 1183 799
Size: 282 102 4622
Fragments arranged by size:
4622 282 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1396 1423 2182 3061 3553 3851 973
Size: 423 27 759 879 492 298 2128
Fragments arranged by size:
2128 879 759 492 423 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 1365 1930 4710 1365
Size: 565 2780 1661
Fragments arranged by size:
2780 1661 565
BsaAI yAC'GTr
Cuts at: 2359 2359
Size: 5006
BsaBI GATnn'nnATC
Cuts at: 6 2520 4557 6
Size: 2514 2037 455
Fragments arranged by size:
2514 2037 455
BsaHI Gr'CG_yC
Cuts at: 1577 1691 2437 2685 3387 4883 1577
Size: 114 746 248 702 1496 1700
Fragments arranged by size:
1700 1496 746 702 248 114
BsaWI w'CCGG_w
Cuts at: 2195 2716 3394 3668 3815 4226 2195
Size: 521 678 274 147 411 2975
Fragments arranged by size:
2975 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1966 1996 1966
Size: 30 4976
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 486 760 1078 1192 1315 211
Size: 275 274 318 114 123 3902
Fragments arranged by size:
3902 318 275 274 123 114
BseYI C'CCAG_C
Cuts at: 137 809 1632 2431 3189 3766 4262 137
Size: 672 823 799 758 577 496 881
Fragments arranged by size:
881 823 799 758 672 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 346 963 1120 1957 211
Size: 135 617 157 837 3260
Fragments arranged by size:
3260 837 617 157 135
BsiEI CG_ry'CG
Cuts at: 104 1502 1626 2548 2594 3802 104
Size: 1398 124 922 46 1208 1308
Fragments arranged by size:
1398 1308 1208 922 124 46
BsiHKAI G_wGCw'C
Cuts at: 130 550 752 1025 1301 1454 1885 2020 2798
Size: 420 202 273 276 153 431 135 778
Cuts at: 2798 2988 3780 130
Size: 190 792 1356
Fragments arranged by size:
1356 792 778 431 420 276 273 202
190 153 135
BsmI GAATG_Cn'
Cuts at: 1998 1998
Size: 5006
BsmBI CGTCTCn'nnnn_
Cuts at: 1564 1698 1564
Size: 134 4872
Bsp1286I G_dGCh'C
Cuts at: 130 138 152 377 550 725 752 771 1025
Size: 8 14 225 173 175 27 19 254
Cuts at: 1025 1301 1454 1782 1885 2020 2631 2724 2798
Size: 276 153 328 103 135 611 93 74
Cuts at: 2798 2988 3050 3780 130
Size: 190 62 730 1356
Fragments arranged by size:
1356 730 611 328 276 254 225 190
175 173 153 135 103 93 74 62
27 19 14 8
BspEI T'CCGG_A
Cuts at: 4226 4226
Size: 5006
BspHI T'CATG_A
Cuts at: 803 827 4182 803
Size: 24 3355 1627
Fragments arranged by size:
3355 1627 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2056 2270 2572 2953 2056
Size: 214 302 381 4109
Fragments arranged by size:
4109 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1336 1658 3298 3352 4836 5001 50
Size: 1286 322 1640 54 1484 165 55
Fragments arranged by size:
1640 1484 1286 322 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2918 2918
Size: 5006
BsrFI r'CCGG_y
Cuts at: 1497 3004 3185 3394 1497
Size: 1507 181 209 3109
Fragments arranged by size:
3109 1507 209 181
BsrGI T'GTAC_A
Cuts at: 1217 1481 1217
Size: 264 4742
BssSI C'ACGA_G
Cuts at: 736 1783 1987 3277 3635 736
Size: 1047 204 1290 358 2107
Fragments arranged by size:
2107 1290 1047 358 204
BstAPI GCAn_nnn'nTGC
Cuts at: 2383 2383
Size: 5006
BstBI TT'CG_AA
Cuts at: 908 3367 908
Size: 2459 2547
BstEII G'GTnAC_C
Cuts at: 1126 1155 1126
Size: 29 4977
BstXI CCAn_nnnn'nTGG
Cuts at: 1893 1893
Size: 5006
BstYI r'GATC_y
Cuts at: 1 527 1196 1394 2031 2498 2521 2856 3102
Size: 526 669 198 637 467 23 335 246
Cuts at: 3102 4114 4200 4212 4558 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 669 637 526 467 449 346 335
246 198 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 536 536
Size: 5006
BtgI C'CryG_G
Cuts at: 308 308
Size: 5006
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 854 1324 2956 3137 3300 854
Size: 470 1632 181 163 2560
Fragments arranged by size:
2560 1632 470 181 163
BtsI GCAGTG_nn'
Cuts at: 178 2249 178
Size: 2071 2935
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DraIII CAC_nnn'GTG
Cuts at: 1020 1020
Size: 5006
DrdI GACnn_nn'nnGTC
Cuts at: 1771 2712 3570 1771
Size: 941 858 3207
Fragments arranged by size:
3207 941 858
EaeI y'GGCC_r
Cuts at: 1091 1331 1499 1904 2591 2765 3156 3183 4528
Size: 240 168 405 687 174 391 27 1345
Cuts at: 4528 1091
Size: 1569
Fragments arranged by size:
1569 1345 687 405 391 240 174 168
27
EagI C'GGCC_G
Cuts at: 1499 2591 1499
Size: 1092 3914
EarI CTCTTCn'nnn_
Cuts at: 190 1952 3029 3239 190
Size: 1762 1077 210 1957
Fragments arranged by size:
1957 1762 1077 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3214 3524 3670 4816 3214
Size: 310 146 1146 3404
Fragments arranged by size:
3404 1146 310 146
EcoICRI GAG'CTC
Cuts at: 750 1299 2018 750
Size: 549 719 3738
Fragments arranged by size:
3738 719 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 799 1081 1183 1752 2831 3263 4010 4278
Size: 621 282 102 569 1079 432 747 268
Cuts at: 4278 178
Size: 906
Fragments arranged by size:
1079 906 747 621 569 432 282 268
102
EcoNI CCTnn'n_nnAGG
Cuts at: 535 535
Size: 5006
EcoO109I rG'GnC_Cy
Cuts at: 149 540 1143 2145 149
Size: 391 603 1002 3010
Fragments arranged by size:
3010 1002 603 391
EcoRI G'AATT_C
Cuts at: 2010 2010
Size: 5006
EcoRV GAT'ATC
Cuts at: 801 1385 801
Size: 584 4422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1284 3025 1284
Size: 1741 3265
FspI TGC'GCA
Cuts at: 319 2787 319
Size: 2468 2538
HaeII r_GCGC'y
Cuts at: 357 1580 2440 2688 3710 4689 357
Size: 1223 860 248 1022 979 674
Fragments arranged by size:
1223 1022 979 860 674 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 519 551 904 936 984 1016 1929
Size: 32 327 32 353 32 48 32 913
Cuts at: 1929 1961 2848 2878 2880 2910 160
Size: 32 887 30 2 30 2256
Fragments arranged by size:
2256 913 887 353 327 48 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 435 2045 435
Size: 1610 3396
KasI G'GCGC_C
Cuts at: 1576 2436 2684 1576
Size: 860 248 3898
Fragments arranged by size:
3898 860 248
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 848 1028 1172 1235 1646 3677 3861 848
Size: 180 144 63 411 2031 184 1993
Fragments arranged by size:
2031 1993 411 184 180 144 63
MscI TGG'CCA
Cuts at: 1093 2767 1093
Size: 1674 3332
MslI CAynn'nnrTG
Cuts at: 487 901 1891 1991 2995 3122 487
Size: 414 990 100 1004 127 2371
Fragments arranged by size:
2371 1004 990 414 127 100
MspA1I CmG'CkG
Cuts at: 2117 2431 2791 3804 4049 2117
Size: 314 360 1013 245 3074
Fragments arranged by size:
3074 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1499 3187 1499
Size: 1688 3318
NarI GG'CG_CC
Cuts at: 1577 2437 2685 1577
Size: 860 248 3898
Fragments arranged by size:
3898 860 248
NcoI C'CATG_G
Cuts at: 308 308
Size: 5006
NgoMIV G'CCGG_C
Cuts at: 1497 3185 1497
Size: 1688 3318
NspI r_CATG'y
Cuts at: 1218 2059 2559 3466 1218
Size: 841 500 907 2758
Fragments arranged by size:
2758 907 841 500
PciI A'CATG_T
Cuts at: 1214 3462 1214
Size: 2248 2758
PfoI T'CCnGG_A
Cuts at: 644 815 848 1199 644
Size: 171 33 351 4451
Fragments arranged by size:
4451 351 171 33
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4974
PpuMI rG'GwC_Cy
Cuts at: 1143 1143
Size: 5006
PshAI GACnn'nnGTC
Cuts at: 1612 1612
Size: 5006
PspOMI G'GGCC_C
Cuts at: 134 148 134
Size: 14 4992
PstI C_TGCA'G
Cuts at: 1526 1704 2053 2738 1526
Size: 178 349 685 3794
Fragments arranged by size:
3794 685 349 178
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 2431 2791 2431
Size: 360 4646
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 752 1301 2020 752
Size: 549 719 3738
Fragments arranged by size:
3738 719 549
SalI G'TCGA_C
Cuts at: 2043 2043
Size: 5006
SapI GCTCTTCn'nnn_
Cuts at: 190 3029 3239 190
Size: 2839 210 1957
Fragments arranged by size:
2839 1957 210
SbfI CC_TGCA'GG
Cuts at: 1526 2053 1526
Size: 527 4479
ScaI AGT'ACT
Cuts at: 1462 4523 1462
Size: 3061 1945
SfcI C'TryA_G
Cuts at: 33 791 1522 1700 2049 2734 3727 3918 33
Size: 758 731 178 349 685 993 191 1121
Fragments arranged by size:
1121 993 758 731 685 349 191 178
SfoI GGC'GCC
Cuts at: 1578 2438 2686 1578
Size: 860 248 3898
Fragments arranged by size:
3898 860 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 133 2028 133
Size: 1895 3111
SmlI C'TyrA_G
Cuts at: 988 1411 1438 2161 3076 3568 3830 988
Size: 423 27 723 915 492 262 2164
Fragments arranged by size:
2164 915 723 492 423 262 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 2059 2559 2059
Size: 500 4506
SspI AAT'ATT
Cuts at: 927 927
Size: 5006
StyI C'CwwG_G
Cuts at: 308 417 1709 2140 2494 308
Size: 109 1292 431 354 2820
Fragments arranged by size:
2820 1292 431 354 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 1602 1903 2589 2722 3181 3390 559
Size: 1043 301 686 133 459 209 2175
Fragments arranged by size:
2175 1043 686 459 301 209 133
TatI w'GTAC_w
Cuts at: 1217 1460 1481 1754 1796 4521 1217
Size: 243 21 273 42 2725 1702
Fragments arranged by size:
2725 1702 273 243 42 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1966 1998 4523 4555 1966
Size: 32 2525 32 2417
Fragments arranged by size:
2525 2417 32 32
Tth111I GACn'n_nGTC
Cuts at: 2803 2803
Size: 5006
XbaI T'CTAG_A
Cuts at: 456 2037 456
Size: 1581 3425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2501
Size: 5006
XmaI C'CCGG_G
Cuts at: 131 2026 131
Size: 1895 3111
ZraI GAC'GTC
Cuts at: 1692 1692
Size: 5006
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BssHII BstZ17I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI