Restriction map for
pF1KA1594
(Circular) (Six-base) MAPSORT of: pF1KA1594.seq Check: 1754 from: 1 to: 6050
pF1KA1594 6050 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 3088 3088
Size: 6050
Acc65I G'GTAC_C
Cuts at: 3066 3066
Size: 6050
AclI AA'CG_TT
Cuts at: 2079 5890 2079
Size: 3811 2239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 2524 2741 2801 3875 4307 4660 5322 139
Size: 2385 217 60 1074 432 353 662 867
Fragments arranged by size:
2385 1074 867 662 432 353 217 60
AflIII A'CryG_T
Cuts at: 3402 5202 5483 3402
Size: 1800 281 3969
Fragments arranged by size:
3969 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 3032 3032
Size: 6050
AleI CACnn'nnGTG
Cuts at: 3016 3016
Size: 6050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1406 1539 1406
Size: 133 5917
AlwNI CAG_nnn'CTG
Cuts at: 1125 2550 2719 3355 4793 1125
Size: 1425 169 636 1438 2382
Fragments arranged by size:
2382 1438 1425 636 169
ApaLI G'TGCA_C
Cuts at: 4888 4888
Size: 6050
ApoI r'AATT_y
Cuts at: 140 245 1241 1399 1488 1759 1974 2011 3054
Size: 105 996 158 89 271 215 37 1043
Cuts at: 3054 5403 5659 140
Size: 2349 256 531
Fragments arranged by size:
2349 1043 996 531 271 256 215 158
105 89 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 3070 3070
Size: 6050
AvrII C'CTAG_G
Cuts at: 1774 1774
Size: 6050
BamHI G'GATC_C
Cuts at: 181 931 2406 3075 181
Size: 750 1475 669 3156
Fragments arranged by size:
3156 1475 750 669
BanI G'GyrC_C
Cuts at: 3066 3480 3728 3763 3066
Size: 414 248 35 5353
Fragments arranged by size:
5353 414 248 35
BanII G_rGCy'C
Cuts at: 2035 3064 4094 2035
Size: 1029 1030 3991
Fragments arranged by size:
3991 1030 1029
BbeI G_GCGC'C
Cuts at: 3484 3732 3484
Size: 248 5802
BbsI GAAGACnn'nnnn_
Cuts at: 1468 2144 2620 3031 1468
Size: 676 476 411 4487
Fragments arranged by size:
4487 676 476 411
BbvCI CC'TCA_GC
Cuts at: 1025 2092 2726 1025
Size: 1067 634 4349
Fragments arranged by size:
4349 1067 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2902 2936 2902
Size: 34 6016
BciVI GTATCCnnnnn_n'
Cuts at: 1237 3948 5004 6050 1237
Size: 2711 1056 1046 1237
Fragments arranged by size:
2711 1237 1056 1046
BclI T'GATC_A
Cuts at: 3570 3570
Size: 6050
BfrBI ATG'CAT
Cuts at: 2659 2659
Size: 6050
BglII A'GATC_T
Cuts at: 2963 3565 5602 2963
Size: 602 2037 3411
Fragments arranged by size:
3411 2037 602
BlpI GC'TnA_GC
Cuts at: 3162 3162
Size: 6050
Bme1580I G_kGCm'C
Cuts at: 1100 3675 3768 4892 1100
Size: 2575 93 1124 2258
Fragments arranged by size:
2575 2258 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 167 293 3262 3343 3678 5822 167
Size: 126 2969 81 335 2144 395
Fragments arranged by size:
2969 2144 395 335 126 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1386 4417 1386
Size: 3031 3019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 798 1177 1499 1729 2450 2482 2902 260
Size: 538 379 322 230 721 32 420 3408
Fragments arranged by size:
3408 721 538 420 379 322 230 32
Bpu10I CC'TnA_GC
Cuts at: 1025 2092 2726 1025
Size: 1067 634 4349
Fragments arranged by size:
4349 1067 634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 3226 4105 4819 5117 1312
Size: 1914 879 714 298 2245
Fragments arranged by size:
2245 1914 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1303 5754 1303
Size: 4451 1599
BsaAI yAC'GTr
Cuts at: 3403 3403
Size: 6050
BsaBI GATnn'nnATC
Cuts at: 6 2924 3564 5601 6
Size: 2918 640 2037 455
Fragments arranged by size:
2918 2037 640 455
BsaHI Gr'CG_yC
Cuts at: 3481 3729 4431 5927 3481
Size: 248 702 1496 3604
Fragments arranged by size:
3604 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2320 3239 3760 4438 4849 4996 5270 2320
Size: 919 521 678 411 147 274 3100
Fragments arranged by size:
3100 919 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 104 1649 104
Size: 1545 4505
BseYI C'CCAG_C
Cuts at: 3475 4233 4898 5306 3475
Size: 758 665 408 4219
Fragments arranged by size:
4219 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2932 2932
Size: 6050
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4868 104
Size: 3488 46 1230 1286
Fragments arranged by size:
3488 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1746 2035 3064 3842 4032 4892 1746
Size: 289 1029 778 190 860 2904
Fragments arranged by size:
2904 1029 860 778 289 190
BsmI GAATG_Cn'
Cuts at: 1170 1170
Size: 6050
BsmBI CGTCTCn'nnnn_
Cuts at: 452 452
Size: 6050
Bsp1286I G_dGCh'C
Cuts at: 1100 1746 2035 3064 3675 3768 3842 4032 4094
Size: 646 289 1029 611 93 74 190 62
Cuts at: 4094 4892 1100
Size: 798 2258
Fragments arranged by size:
2258 1029 798 646 611 289 190 93
74 62
BspHI T'CATG_A
Cuts at: 1395 2283 4482 1395
Size: 888 2199 2963
Fragments arranged by size:
2963 2199 888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 3100 3314 3616 3997 1162
Size: 1938 214 302 381 3215
Fragments arranged by size:
3215 1938 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4342 4396 5880 6045 50
Size: 4292 54 1484 165 55
Fragments arranged by size:
4292 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1884 3962 1884
Size: 2078 3972
BsrFI r'CCGG_y
Cuts at: 2774 4048 4229 2774
Size: 1274 181 4595
Fragments arranged by size:
4595 1274 181
BssSI C'ACGA_G
Cuts at: 4321 5029 4321
Size: 708 5342
BstAPI GCAn_nnn'nTGC
Cuts at: 1125 3427 1125
Size: 2302 3748
BstBI TT'CG_AA
Cuts at: 144 585 4411 144
Size: 441 3826 1783
Fragments arranged by size:
3826 1783 441
BstYI r'GATC_y
Cuts at: 1 181 807 931 2406 2473 2963 3075 3542
Size: 180 626 124 1475 67 490 112 467
Cuts at: 3542 3565 3900 4146 4452 4464 4550 5602 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1475 1052 626 490 467 449 335 306
246 180 124 112 86 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 767 767
Size: 6050
BtgI C'CryG_G
Cuts at: 1231 1231
Size: 6050
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 3293 3293
Size: 6050
ClaI AT'CG_AT
Cuts at: 616 616
Size: 6050
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 3756 5100 3756
Size: 1344 4706
EaeI y'GGCC_r
Cuts at: 3635 3809 4200 4227 5572 3635
Size: 174 391 27 1345 4113
Fragments arranged by size:
4113 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 863 951 2219 2522 4073 4283 863
Size: 88 1268 303 1551 210 2630
Fragments arranged by size:
2630 1551 1268 303 210 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4258 5000 5146 5860 4258
Size: 742 146 714 4448
Fragments arranged by size:
4448 742 714 146
EcoICRI GAG'CTC
Cuts at: 2033 3062 2033
Size: 1029 5021
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 260 798 1177 1499 1729 2450 2482 2524
Size: 121 538 379 322 230 721 32 42
Cuts at: 2524 2741 2801 2902 3875 4307 4660 5322 139
Size: 217 60 101 973 432 353 662 867
Fragments arranged by size:
973 867 721 662 538 432 379 353
322 230 217 121 101 60 42 32
EcoO109I rG'GnC_Cy
Cuts at: 488 1779 3189 488
Size: 1291 1410 3349
Fragments arranged by size:
3349 1410 1291
EcoRI G'AATT_C
Cuts at: 140 245 1974 3054 140
Size: 105 1729 1080 3136
Fragments arranged by size:
3136 1729 1080 105
EcoRV GAT'ATC
Cuts at: 734 1296 734
Size: 562 5488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 2052 2245 2982 4069 1696
Size: 356 193 737 1087 3677
Fragments arranged by size:
3677 1087 737 356 193
FspI TGC'GCA
Cuts at: 1931 3831 1931
Size: 1900 4150
FspAI rTGC'GCAy
Cuts at: 1931 1931
Size: 6050
HaeII r_GCGC'y
Cuts at: 3484 3732 4962 5733 3484
Size: 248 1230 771 3801
Fragments arranged by size:
3801 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 769 801 1577 1609 2662 2694 2742
Size: 32 623 32 776 32 1053 32 48
Cuts at: 2742 2774 3018 3050 3892 3922 3924 3954 114
Size: 32 244 32 842 30 2 30 2210
Fragments arranged by size:
2210 1053 842 776 623 244 48 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 329 3089 329
Size: 2760 3290
HpaI GTT'AAC
Cuts at: 329 329
Size: 6050
KasI G'GCGC_C
Cuts at: 3480 3728 3480
Size: 248 5802
KpnI G_GTAC'C
Cuts at: 3070 3070
Size: 6050
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1903 4809 4993 1903
Size: 2906 184 2960
Fragments arranged by size:
2960 2906 184
MscI TGG'CCA
Cuts at: 3811 3811
Size: 6050
MslI CAynn'nnrTG
Cuts at: 2288 3016 4039 4166 2288
Size: 728 1023 127 4172
Fragments arranged by size:
4172 1023 728 127
MspA1I CmG'CkG
Cuts at: 849 1425 2239 3161 3475 3835 4619 4864 849
Size: 576 814 922 314 360 784 245 2035
Fragments arranged by size:
2035 922 814 784 576 360 314 245
NaeI GCC'GGC
Cuts at: 4231 4231
Size: 6050
NarI GG'CG_CC
Cuts at: 3481 3729 3481
Size: 248 5802
NcoI C'CATG_G
Cuts at: 1231 1231
Size: 6050
NdeI CA'TA_TG
Cuts at: 1934 1934
Size: 6050
NgoMIV G'CCGG_C
Cuts at: 4229 4229
Size: 6050
NsiI A_TGCA'T
Cuts at: 2661 2661
Size: 6050
NspI r_CATG'y
Cuts at: 1583 3103 3603 5206 1583
Size: 1520 500 1603 2427
Fragments arranged by size:
2427 1603 1520 500
PciI A'CATG_T
Cuts at: 5202 5202
Size: 6050
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 488 1779 488
Size: 1291 4759
PstI C_TGCA'G
Cuts at: 435 1130 3097 3782 435
Size: 695 1967 685 2703
Fragments arranged by size:
2703 1967 695 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 849 1425 2239 3475 3835 849
Size: 576 814 1236 360 3064
Fragments arranged by size:
3064 1236 814 576 360
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 2035 3064 2035
Size: 1029 5021
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 2219 2522 4073 4283 2219
Size: 303 1551 210 3986
Fragments arranged by size:
3986 1551 303 210
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 5567 5567
Size: 6050
SfcI C'TryA_G
Cuts at: 33 431 626 1126 1355 3093 3778 4746 4937
Size: 398 195 500 229 1738 685 968 191
Cuts at: 4937 33
Size: 1146
Fragments arranged by size:
1738 1146 968 685 500 398 229 195
191
SfoI GGC'GCC
Cuts at: 3482 3730 3482
Size: 248 5802
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 3072 3072
Size: 6050
SmlI C'TyrA_G
Cuts at: 1327 3205 4120 4834 5096 1327
Size: 1878 915 714 262 2281
Fragments arranged by size:
2281 1878 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 3103 3603 3103
Size: 500 5550
StuI AGG'CCT
Cuts at: 3040 3040
Size: 6050
StyI C'CwwG_G
Cuts at: 250 935 1231 1774 1917 2036 3184 3538 250
Size: 685 296 543 143 119 1148 354 2762
Fragments arranged by size:
2762 1148 685 543 354 296 143 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3633 3766 4225 4434 3633
Size: 133 459 209 5249
Fragments arranged by size:
5249 459 209 133
TatI w'GTAC_w
Cuts at: 2708 5565 2708
Size: 2857 3193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5567 5599 5567
Size: 32 6018
Tth111I GACn'n_nGTC
Cuts at: 1875 3847 1875
Size: 1972 4078
XbaI T'CTAG_A
Cuts at: 402 1945 3081 402
Size: 1543 1136 3371
Fragments arranged by size:
3371 1543 1136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1008 1072 2034 3545 1008
Size: 64 962 1511 3513
Fragments arranged by size:
3513 1511 962 64
XmaI C'CCGG_G
Cuts at: 3070 3070
Size: 6050
XmnI GAAnn'nnTTC
Cuts at: 2228 2228
Size: 6050
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI ApaI AscI
BaeI BglI BmgBI BmtI BsaXI BsiWI BspEI BsrGI
BssHII BstEII BstXI BstZ17I CspCI DraIII EcoNI FseI
HindIII MfeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI