Restriction map for
pF1KA1597
(Circular) (Six-base) MAPSORT of: pF1KA1597.seq Check: 4051 from: 1 to: 5798
pF1KA1597 5798 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2836 2836
Size: 5798
Acc65I G'GTAC_C
Cuts at: 2246 2814 2246
Size: 568 5230
AclI AA'CG_TT
Cuts at: 5638 5638
Size: 5798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 220 1691 1936 2275 2573 2675 2687 3623
Size: 63 1471 245 339 298 102 12 936
Cuts at: 3623 4055 4408 5070 157
Size: 432 353 662 885
Fragments arranged by size:
1471 936 885 662 432 353 339 298
245 102 63 12
AflIII A'CryG_T
Cuts at: 3150 4950 5231 3150
Size: 1800 281 3717
Fragments arranged by size:
3717 1800 281
AleI CACnn'nnGTG
Cuts at: 2530 2530
Size: 5798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 143 143
Size: 5798
AlwNI CAG_nnn'CTG
Cuts at: 198 2341 3103 4541 198
Size: 2143 762 1438 1455
Fragments arranged by size:
2143 1455 1438 762
ApaLI G'TGCA_C
Cuts at: 2371 4636 2371
Size: 2265 3533
ApoI r'AATT_y
Cuts at: 989 1126 1368 1724 2427 2802 5151 5407 989
Size: 137 242 356 703 375 2349 256 1380
Fragments arranged by size:
2349 1380 703 375 356 256 242 137
AseI AT'TA_AT
Cuts at: 20 1313 20
Size: 1293 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
AvaI C'yCGr_G
Cuts at: 447 2818 447
Size: 2371 3427
AvrII C'CTAG_G
Cuts at: 2178 2178
Size: 5798
BamHI G'GATC_C
Cuts at: 875 2823 875
Size: 1948 3850
BanI G'GyrC_C
Cuts at: 1488 2246 2814 3228 3476 3511 1488
Size: 758 568 414 248 35 3775
Fragments arranged by size:
3775 758 568 414 248 35
BanII G_rGCy'C
Cuts at: 867 2812 3842 867
Size: 1945 1030 2823
Fragments arranged by size:
2823 1945 1030
BbeI G_GCGC'C
Cuts at: 3232 3480 3232
Size: 248 5550
BbsI GAAGACnn'nnnn_
Cuts at: 1022 1607 2269 2488 2620 1022
Size: 585 662 219 132 4200
Fragments arranged by size:
4200 662 585 219 132
BciVI GTATCCnnnnn_n'
Cuts at: 2148 3696 4752 5798 2148
Size: 1548 1056 1046 2148
Fragments arranged by size:
2148 1548 1056 1046
BclI T'GATC_A
Cuts at: 3318 3318
Size: 5798
BglII A'GATC_T
Cuts at: 2556 3313 5350 2556
Size: 757 2037 3004
Fragments arranged by size:
3004 2037 757
BlpI GC'TnA_GC
Cuts at: 317 2910 317
Size: 2593 3205
Bme1580I G_kGCm'C
Cuts at: 2375 3423 3516 4640 2375
Size: 1048 93 1124 3533
Fragments arranged by size:
3533 1124 1048 93
BmrI ACTGGGnnnn_n'
Cuts at: 1532 1935 2223 3010 3091 3426 5570 1532
Size: 403 288 787 81 335 2144 1760
Fragments arranged by size:
2144 1760 787 403 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 881 895 4165 881
Size: 14 3270 2514
Fragments arranged by size:
3270 2514 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 509 876 1060 2302 2384 377
Size: 132 367 184 1242 82 3791
Fragments arranged by size:
3791 1242 367 184 132 82
Bpu10I CC'TnA_GC
Cuts at: 1205 1475 2410 1205
Size: 270 935 4593
Fragments arranged by size:
4593 935 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1236 2974 3853 4567 4865 865
Size: 371 1738 879 714 298 1798
Fragments arranged by size:
1798 1738 879 714 371 298
BsaI GGTCTCn'nnnn_
Cuts at: 1502 5502 1502
Size: 4000 1798
BsaAI yAC'GTr
Cuts at: 3151 3151
Size: 5798
BsaBI GATnn'nnATC
Cuts at: 6 3312 5349 6
Size: 3306 2037 455
Fragments arranged by size:
3306 2037 455
BsaHI Gr'CG_yC
Cuts at: 3229 3477 4179 5675 3229
Size: 248 702 1496 3352
Fragments arranged by size:
3352 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2987 3508 4186 4597 4744 5018 2987
Size: 521 678 411 147 274 3767
Fragments arranged by size:
3767 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2300 2330 2300
Size: 30 5768
BseYI C'CCAG_C
Cuts at: 356 3223 3981 4646 5054 356
Size: 2867 758 665 408 1100
Fragments arranged by size:
2867 1100 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3340 3386 4616 104
Size: 3236 46 1230 1286
Fragments arranged by size:
3236 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2375 2812 3590 3780 4640 2375
Size: 437 778 190 860 3533
Fragments arranged by size:
3533 860 778 437 190
BsmI GAATG_Cn'
Cuts at: 1005 1323 1717 2395 2771 1005
Size: 318 394 678 376 4032
Fragments arranged by size:
4032 678 394 376 318
Bsp1286I G_dGCh'C
Cuts at: 867 2375 2812 3423 3516 3590 3780 3842 4640
Size: 1508 437 611 93 74 190 62 798
Cuts at: 4640 867
Size: 2025
Fragments arranged by size:
2025 1508 798 611 437 190 93 74
62
BspHI T'CATG_A
Cuts at: 4230 4230
Size: 5798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2848 3062 3364 3745 2848
Size: 214 302 381 4901
Fragments arranged by size:
4901 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4090 4144 5628 5793 50
Size: 4040 54 1484 165 55
Fragments arranged by size:
4040 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3710 3710
Size: 5798
BsrFI r'CCGG_y
Cuts at: 3796 3977 3796
Size: 181 5617
BssSI C'ACGA_G
Cuts at: 4069 4777 4069
Size: 708 5090
BstAPI GCAn_nnn'nTGC
Cuts at: 723 2275 3175 723
Size: 1552 900 3346
Fragments arranged by size:
3346 1552 900
BstBI TT'CG_AA
Cuts at: 4159 4159
Size: 5798
BstYI r'GATC_y
Cuts at: 1 875 2556 2823 3290 3313 3648 3894 4200
Size: 874 1681 267 467 23 335 246 306
Cuts at: 4200 4212 4298 5350 1
Size: 12 86 1052 449
Fragments arranged by size:
1681 1052 874 467 449 335 306 267
246 86 23 12
BtgI C'CryG_G
Cuts at: 261 1107 261
Size: 846 4952
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3748 3929 4092 3748
Size: 181 163 5454
Fragments arranged by size:
5454 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1836 2404 2752 3041 1076
Size: 760 568 348 289 3833
Fragments arranged by size:
3833 760 568 348 289
DraI TTT'AAA
Cuts at: 803 2797 803
Size: 1994 3804
DrdI GACnn_nn'nnGTC
Cuts at: 3504 4848 3504
Size: 1344 4454
EaeI y'GGCC_r
Cuts at: 213 3383 3557 3948 3975 5320 213
Size: 3170 174 391 27 1345 691
Fragments arranged by size:
3170 1345 691 391 174 27
EagI C'GGCC_G
Cuts at: 3383 3383
Size: 5798
EarI CTCTTCn'nnn_
Cuts at: 133 146 292 410 419 526 1029 1181 3821
Size: 13 146 118 9 107 503 152 2640
Cuts at: 3821 4031 133
Size: 210 1900
Fragments arranged by size:
2640 1900 503 210 152 146 118 107
13 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4006 4748 4894 5608 4006
Size: 742 146 714 4196
Fragments arranged by size:
4196 742 714 146
EcoICRI GAG'CTC
Cuts at: 2810 2810
Size: 5798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 220 377 509 876 1060 1691 1936 2275
Size: 63 157 132 367 184 631 245 339
Cuts at: 2275 2302 2384 2573 2675 2687 3623 4055 4408
Size: 27 82 189 102 12 936 432 353
Cuts at: 4408 5070 157
Size: 662 885
Fragments arranged by size:
936 885 662 631 432 367 353 339
245 189 184 157 132 102 82 63
27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2182 2182
Size: 5798
EcoO109I rG'GnC_Cy
Cuts at: 302 743 2937 302
Size: 441 2194 3163
Fragments arranged by size:
3163 2194 441
EcoRI G'AATT_C
Cuts at: 1368 2802 1368
Size: 1434 4364
EcoRV GAT'ATC
Cuts at: 273 273
Size: 5798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2079 3817 2079
Size: 1738 4060
FspI TGC'GCA
Cuts at: 3579 3579
Size: 5798
HaeII r_GCGC'y
Cuts at: 3232 3480 4710 5481 3232
Size: 248 1230 771 3549
Fragments arranged by size:
3549 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 525 557 637 669 805 837 1387 1419 1441
Size: 32 80 32 136 32 550 32 22
Cuts at: 1441 1473 1594 1626 3640 3670 3672 3702 525
Size: 32 121 32 2014 30 2 30 2621
Fragments arranged by size:
2621 2014 550 136 121 80 32 32
32 32 32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 372 674 2837 372
Size: 302 2163 3333
Fragments arranged by size:
3333 2163 302
HindIII A'AGCT_T
Cuts at: 2350 2350
Size: 5798
KasI G'GCGC_C
Cuts at: 3228 3476 3228
Size: 248 5550
KpnI G_GTAC'C
Cuts at: 2250 2818 2250
Size: 568 5230
MfeI C'AATT_G
Cuts at: 1895 2236 1895
Size: 341 5457
MluI A'CGCG_T
Cuts at: 5231 5231
Size: 5798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4557 4741 4557
Size: 184 5614
MscI TGG'CCA
Cuts at: 215 3559 215
Size: 3344 2454
MslI CAynn'nnrTG
Cuts at: 2380 2530 3787 3914 2380
Size: 150 1257 127 4264
Fragments arranged by size:
4264 1257 150 127
MspA1I CmG'CkG
Cuts at: 123 198 316 1963 2909 3223 3583 4367 4612
Size: 75 118 1647 946 314 360 784 245
Cuts at: 4612 123
Size: 1309
Fragments arranged by size:
1647 1309 946 784 360 314 245 118
75
NaeI GCC'GGC
Cuts at: 3979 3979
Size: 5798
NarI GG'CG_CC
Cuts at: 3229 3477 3229
Size: 248 5550
NcoI C'CATG_G
Cuts at: 261 1107 261
Size: 846 4952
NdeI CA'TA_TG
Cuts at: 1260 1992 1260
Size: 732 5066
NgoMIV G'CCGG_C
Cuts at: 3977 3977
Size: 5798
NspI r_CATG'y
Cuts at: 1057 1930 2851 3351 4954 1057
Size: 873 921 500 1603 1901
Fragments arranged by size:
1901 1603 921 873 500
PciI A'CATG_T
Cuts at: 4950 4950
Size: 5798
PflMI CCAn_nnn'nTGG
Cuts at: 1315 2341 2529 2619 2738 1315
Size: 1026 188 90 119 4375
Fragments arranged by size:
4375 1026 188 119 90
PmeI GTTT'AAAC
Cuts at: 2797 2797
Size: 5798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 5766
PpuMI rG'GwC_Cy
Cuts at: 743 743
Size: 5798
PshAI GACnn'nnGTC
Cuts at: 1356 1356
Size: 5798
PsiI TTA'TAA
Cuts at: 929 929
Size: 5798
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2527 2559 2527
Size: 32 5766
PstI C_TGCA'G
Cuts at: 1071 2845 3530 1071
Size: 1774 685 3339
Fragments arranged by size:
3339 1774 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5798
PvuII CAG'CTG
Cuts at: 198 316 3223 3583 198
Size: 118 2907 360 2413
Fragments arranged by size:
2907 2413 360 118
RsrII CG'GwC_CG
Cuts at: 3993 3993
Size: 5798
SacI G_AGCT'C
Cuts at: 2812 2812
Size: 5798
SalI G'TCGA_C
Cuts at: 2835 2835
Size: 5798
SanDI GG'GwC_CC
Cuts at: 743 743
Size: 5798
SapI GCTCTTCn'nnn_
Cuts at: 410 3821 4031 410
Size: 3411 210 2177
Fragments arranged by size:
3411 2177 210
SbfI CC_TGCA'GG
Cuts at: 2845 2845
Size: 5798
ScaI AGT'ACT
Cuts at: 5315 5315
Size: 5798
SfcI C'TryA_G
Cuts at: 33 1067 2492 2841 3526 4494 4685 33
Size: 1034 1425 349 685 968 191 1146
Fragments arranged by size:
1425 1146 1034 968 685 349 191
SfoI GGC'GCC
Cuts at: 3230 3478 3230
Size: 248 5550
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
SmaI CCC'GGG
Cuts at: 2820 2820
Size: 5798
SmlI C'TyrA_G
Cuts at: 880 1251 2953 3868 4582 4844 880
Size: 371 1702 915 714 262 1834
Fragments arranged by size:
1834 1702 915 714 371 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5798
SphI G_CATG'C
Cuts at: 2851 3351 2851
Size: 500 5298
SspI AAT'ATT
Cuts at: 1884 1884
Size: 5798
StuI AGG'CCT
Cuts at: 731 2002 2550 731
Size: 1271 548 3979
Fragments arranged by size:
3979 1271 548
StyI C'CwwG_G
Cuts at: 233 261 1107 2178 2932 3286 233
Size: 28 846 1071 754 354 2745
Fragments arranged by size:
2745 1071 846 754 354 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 2370 3381 3514 3973 4182 348
Size: 2022 1011 133 459 209 1964
Fragments arranged by size:
2022 1964 1011 459 209 133
TatI w'GTAC_w
Cuts at: 5313 5313
Size: 5798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5315 5347 5315
Size: 32 5766
Tth111I GACn'n_nGTC
Cuts at: 3595 3595
Size: 5798
XbaI T'CTAG_A
Cuts at: 1504 1596 2829 1504
Size: 92 1233 4473
Fragments arranged by size:
4473 1233 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2616 3293 2616
Size: 677 5121
XmaI C'CCGG_G
Cuts at: 2818 2818
Size: 5798
XmnI GAAnn'nnTTC
Cuts at: 920 1190 1227 920
Size: 270 37 5491
Fragments arranged by size:
5491 270 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI