Restriction map for
pF1KA1611
(Circular) (Six-base) MAPSORT of: pF1KA1611.seq Check: 5029 from: 1 to: 5567
pF1KA1611 5567 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 736 2605 736
Size: 1869 3698
Acc65I G'GTAC_C
Cuts at: 2583 2583
Size: 5567
AclI AA'CG_TT
Cuts at: 5407 5407
Size: 5567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1056 1476 1644 2232 2484 3392 3824 4177 4839
Size: 420 168 588 252 908 432 353 662
Cuts at: 4839 1056
Size: 1784
Fragments arranged by size:
1784 908 662 588 432 420 353 252
168
AflIII A'CryG_T
Cuts at: 2919 4719 5000 2919
Size: 1800 281 3486
Fragments arranged by size:
3486 1800 281
AleI CACnn'nnGTG
Cuts at: 454 454
Size: 5567
AlwNI CAG_nnn'CTG
Cuts at: 2872 4310 2872
Size: 1438 4129
ApaLI G'TGCA_C
Cuts at: 4405 4405
Size: 5567
ApoI r'AATT_y
Cuts at: 154 773 912 938 1106 1248 1274 1584 1610
Size: 619 139 26 168 142 26 310 26
Cuts at: 1610 1778 2030 2366 2571 4920 5176 154
Size: 168 252 336 205 2349 256 545
Fragments arranged by size:
2349 619 545 336 310 256 252 205
168 168 142 139 26 26 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5567
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
AvaI C'yCGr_G
Cuts at: 2587 2587
Size: 5567
BamHI G'GATC_C
Cuts at: 2592 2592
Size: 5567
BanI G'GyrC_C
Cuts at: 2583 2997 3245 3280 2583
Size: 414 248 35 4870
Fragments arranged by size:
4870 414 248 35
BanII G_rGCy'C
Cuts at: 305 2581 3611 305
Size: 2276 1030 2261
Fragments arranged by size:
2276 2261 1030
BbeI G_GCGC'C
Cuts at: 3001 3249 3001
Size: 248 5319
BbsI GAAGACnn'nnnn_
Cuts at: 494 1062 1818 2154 2490 494
Size: 568 756 336 336 3571
Fragments arranged by size:
3571 756 568 336 336
BciVI GTATCCnnnnn_n'
Cuts at: 3465 4521 5567 3465
Size: 1056 1046 3465
Fragments arranged by size:
3465 1056 1046
BclI T'GATC_A
Cuts at: 3087 3087
Size: 5567
BfrBI ATG'CAT
Cuts at: 1500 2088 2538 1500
Size: 588 450 4529
Fragments arranged by size:
4529 588 450
BglII A'GATC_T
Cuts at: 3082 5119 3082
Size: 2037 3530
BlpI GC'TnA_GC
Cuts at: 2679 2679
Size: 5567
Bme1580I G_kGCm'C
Cuts at: 3192 3285 4409 3192
Size: 93 1124 4350
Fragments arranged by size:
4350 1124 93
BmgBI CAC'GTC
Cuts at: 213 213
Size: 5567
BmrI ACTGGGnnnn_n'
Cuts at: 2300 2384 2779 2860 3195 5339 2300
Size: 84 395 81 335 2144 2528
Fragments arranged by size:
2528 2144 395 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1763 3934 1763
Size: 2171 3396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1136 1220 1304 1388 1640 1724 1808 1892
Size: 84 84 84 84 252 84 84 84
Cuts at: 1892 1976 2060 2144 2480 1052
Size: 84 84 84 336 4139
Fragments arranged by size:
4139 336 252 84 84 84 84 84
84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2743 3622 4336 4634 2743
Size: 879 714 298 3676
Fragments arranged by size:
3676 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5271 5271
Size: 5567
BsaAI yAC'GTr
Cuts at: 2920 2920
Size: 5567
BsaBI GATnn'nnATC
Cuts at: 6 3081 5118 6
Size: 3075 2037 455
Fragments arranged by size:
3075 2037 455
BsaHI Gr'CG_yC
Cuts at: 2998 3246 3948 5444 2998
Size: 248 702 1496 3121
Fragments arranged by size:
3121 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2206 2542 2756 3277 3955 4366 4513 4787 2206
Size: 336 214 521 678 411 147 274 2986
Fragments arranged by size:
2986 678 521 411 336 274 214 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 5567
BseYI C'CCAG_C
Cuts at: 2992 3750 4415 4823 2992
Size: 758 665 408 3736
Fragments arranged by size:
3736 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3109 3155 4385 104
Size: 3005 46 1230 1286
Fragments arranged by size:
3005 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2581 3359 3549 4409 2581
Size: 778 190 860 3739
Fragments arranged by size:
3739 860 778 190
BsmI GAATG_Cn'
Cuts at: 1500 1567 1987 2088 2540 1500
Size: 67 420 101 452 4527
Fragments arranged by size:
4527 452 420 101 67
Bsp1286I G_dGCh'C
Cuts at: 305 2581 3192 3285 3359 3549 3611 4409 305
Size: 2276 611 93 74 190 62 798 1463
Fragments arranged by size:
2276 1463 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 813 1053 1977 3999 813
Size: 240 924 2022 2381
Fragments arranged by size:
2381 2022 924 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2617 2831 3133 3514 2617
Size: 214 302 381 4670
Fragments arranged by size:
4670 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3859 3913 5397 5562 50
Size: 3809 54 1484 165 55
Fragments arranged by size:
3809 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1311 1563 2235 3479 1311
Size: 252 672 1244 3399
Fragments arranged by size:
3399 1244 672 252
BsrFI r'CCGG_y
Cuts at: 3565 3746 3565
Size: 181 5386
BsrGI T'GTAC_A
Cuts at: 394 2395 394
Size: 2001 3566
BssSI C'ACGA_G
Cuts at: 3838 4546 3838
Size: 708 4859
BstAPI GCAn_nnn'nTGC
Cuts at: 974 1310 1562 2944 974
Size: 336 252 1382 3597
Fragments arranged by size:
3597 1382 336 252
BstBI TT'CG_AA
Cuts at: 916 3928 916
Size: 3012 2555
BstYI r'GATC_y
Cuts at: 1 197 2592 3059 3082 3417 3663 3969 3981
Size: 196 2395 467 23 335 246 306 12
Cuts at: 3981 4067 5119 1
Size: 86 1052 449
Fragments arranged by size:
2395 1052 467 449 335 306 246 196
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3517 3698 3861 3517
Size: 181 163 5223
Fragments arranged by size:
5223 181 163
BtsI GCAGTG_nn'
Cuts at: 975 2810 975
Size: 1835 3732
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2217 2252 2217
Size: 35 5532
DraI TTT'AAA
Cuts at: 2566 2566
Size: 5567
DraIII CAC_nnn'GTG
Cuts at: 453 453
Size: 5567
DrdI GACnn_nn'nnGTC
Cuts at: 3273 4617 3273
Size: 1344 4223
EaeI y'GGCC_r
Cuts at: 146 3152 3326 3717 3744 5089 146
Size: 3006 174 391 27 1345 624
Fragments arranged by size:
3006 1345 624 391 174 27
EagI C'GGCC_G
Cuts at: 3152 3152
Size: 5567
EarI CTCTTCn'nnn_
Cuts at: 310 418 609 1243 3590 3800 310
Size: 108 191 634 2347 210 2077
Fragments arranged by size:
2347 2077 634 210 191 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3775 4517 4663 5377 3775
Size: 742 146 714 3965
Fragments arranged by size:
3965 742 714 146
EcoICRI GAG'CTC
Cuts at: 2579 2579
Size: 5567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1056 1136 1220 1304 1388 1476 1640 1644
Size: 4 80 84 84 84 88 164 4
Cuts at: 1644 1724 1808 1892 1976 2060 2144 2232 2480
Size: 80 84 84 84 84 84 88 248
Cuts at: 2480 2484 3392 3824 4177 4839 1052
Size: 4 908 432 353 662 1780
Fragments arranged by size:
1780 908 662 432 353 248 164 88
88 84 84 84 84 84 84 84
84 80 80 4 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 118 118
Size: 5567
EcoO109I rG'GnC_Cy
Cuts at: 2706 2706
Size: 5567
EcoRI G'AATT_C
Cuts at: 154 912 938 1106 1274 1610 1778 2030 2366
Size: 758 26 168 168 336 168 252 336
Cuts at: 2366 2571 154
Size: 205 3150
Fragments arranged by size:
3150 758 336 336 252 205 168 168
168 26
EcoRV GAT'ATC
Cuts at: 381 381
Size: 5567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1949 3586 1949
Size: 1637 3930
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5567
HaeII r_GCGC'y
Cuts at: 3001 3249 4479 5250 3001
Size: 248 1230 771 3318
Fragments arranged by size:
3318 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1671 1703 2499 2531 3409 3439 3441 3471 1671
Size: 32 796 32 878 30 2 30 3767
Fragments arranged by size:
3767 878 796 32 32 30 30 2
HincII GTy'rAC
Cuts at: 131 2606 131
Size: 2475 3092
HindIII A'AGCT_T
Cuts at: 631 657 631
Size: 26 5541
KasI G'GCGC_C
Cuts at: 2997 3245 2997
Size: 248 5319
KpnI G_GTAC'C
Cuts at: 2587 2587
Size: 5567
MluI A'CGCG_T
Cuts at: 5000 5000
Size: 5567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 266 440 4326 4510 200
Size: 66 174 3886 184 1257
Fragments arranged by size:
3886 1257 184 174 66
MscI TGG'CCA
Cuts at: 148 3328 148
Size: 3180 2387
MslI CAynn'nnrTG
Cuts at: 454 1058 1451 3556 3683 454
Size: 604 393 2105 127 2338
Fragments arranged by size:
2338 2105 604 393 127
MspA1I CmG'CkG
Cuts at: 2678 2992 3352 4136 4381 2678
Size: 314 360 784 245 3864
Fragments arranged by size:
3864 784 360 314 245
NaeI GCC'GGC
Cuts at: 3748 3748
Size: 5567
NarI GG'CG_CC
Cuts at: 2998 3246 2998
Size: 248 5319
NcoI C'CATG_G
Cuts at: 107 107
Size: 5567
NdeI CA'TA_TG
Cuts at: 2444 2444
Size: 5567
NgoMIV G'CCGG_C
Cuts at: 3746 3746
Size: 5567
NsiI A_TGCA'T
Cuts at: 1502 2090 2540 1502
Size: 588 450 4529
Fragments arranged by size:
4529 588 450
NspI r_CATG'y
Cuts at: 2620 3120 4723 2620
Size: 500 1603 3464
Fragments arranged by size:
3464 1603 500
PciI A'CATG_T
Cuts at: 4719 4719
Size: 5567
PflMI CCAn_nnn'nTGG
Cuts at: 453 453
Size: 5567
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 5535
PsiI TTA'TAA
Cuts at: 878 1261 878
Size: 383 5184
PstI C_TGCA'G
Cuts at: 686 2614 3299 686
Size: 1928 685 2954
Fragments arranged by size:
2954 1928 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5567
PvuII CAG'CTG
Cuts at: 2992 3352 2992
Size: 360 5207
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5567
SacI G_AGCT'C
Cuts at: 2581 2581
Size: 5567
SalI G'TCGA_C
Cuts at: 2604 2604
Size: 5567
SapI GCTCTTCn'nnn_
Cuts at: 310 418 1243 3590 3800 310
Size: 108 825 2347 210 2077
Fragments arranged by size:
2347 2077 825 210 108
SbfI CC_TGCA'GG
Cuts at: 2614 2614
Size: 5567
ScaI AGT'ACT
Cuts at: 5084 5084
Size: 5567
SfcI C'TryA_G
Cuts at: 33 682 2610 3295 4263 4454 33
Size: 649 1928 685 968 191 1146
Fragments arranged by size:
1928 1146 968 685 649 191
SfoI GGC'GCC
Cuts at: 2999 3247 2999
Size: 248 5319
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
SmaI CCC'GGG
Cuts at: 2589 2589
Size: 5567
SmlI C'TyrA_G
Cuts at: 2722 3637 4351 4613 2722
Size: 915 714 262 3676
Fragments arranged by size:
3676 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5567
SphI G_CATG'C
Cuts at: 2620 3120 2620
Size: 500 5067
SspI AAT'ATT
Cuts at: 273 273
Size: 5567
StyI C'CwwG_G
Cuts at: 107 2701 3055 107
Size: 2594 354 2619
Fragments arranged by size:
2619 2594 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2287 3150 3283 3742 3951 2287
Size: 863 133 459 209 3903
Fragments arranged by size:
3903 863 459 209 133
TatI w'GTAC_w
Cuts at: 394 430 2395 5082 394
Size: 36 1965 2687 879
Fragments arranged by size:
2687 1965 879 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5084 5116 5084
Size: 32 5535
Tth111I GACn'n_nGTC
Cuts at: 254 3364 254
Size: 3110 2457
XbaI T'CTAG_A
Cuts at: 2598 2598
Size: 5567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 3062
Size: 5567
XmaI C'CCGG_G
Cuts at: 2587 2587
Size: 5567
XmnI GAAnn'nnTTC
Cuts at: 1657 1782 2245 1657
Size: 125 463 4979
Fragments arranged by size:
4979 463 125
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI