Restriction map for
pF1KA1625
(Circular) (Six-base) MAPSORT of: pF1KA1625.seq Check: 3115 from: 1 to: 5384
pF1KA1625 5384 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2422 2422
Size: 5384
Acc65I G'GTAC_C
Cuts at: 2400 2400
Size: 5384
AclI AA'CG_TT
Cuts at: 5224 5224
Size: 5384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 712 1264 1987 2074 3209 3641 4388 4656
Size: 529 552 723 87 1135 432 747 268
Cuts at: 4656 183
Size: 911
Fragments arranged by size:
1135 911 747 723 552 529 432 268
87
AflIII A'CryG_T
Cuts at: 1520 2736 3840 4817 1520
Size: 1216 1104 977 2087
Fragments arranged by size:
2087 1216 1104 977
AgeI A'CCGG_T
Cuts at: 664 3772 664
Size: 3108 2276
AhdI GACnn_n'nnGTC
Cuts at: 1563 2037 1563
Size: 474 4910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1852 2098 1852
Size: 246 5138
AlwNI CAG_nnn'CTG
Cuts at: 484 1063 1173 1251 1662 2689 4256 484
Size: 579 110 78 411 1027 1567 1612
Fragments arranged by size:
1612 1567 1027 579 411 110 78
ApaI G_GGCC'C
Cuts at: 2219 2219
Size: 5384
ApaLI G'TGCA_C
Cuts at: 4154 4154
Size: 5384
ApoI r'AATT_y
Cuts at: 362 958 1399 2388 4737 4993 362
Size: 596 441 989 2349 256 753
Fragments arranged by size:
2349 989 753 596 441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5384
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5384
AvaI C'yCGr_G
Cuts at: 118 918 2404 118
Size: 800 1486 3098
Fragments arranged by size:
3098 1486 800
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 435 468 435
Size: 33 5351
BamHI G'GATC_C
Cuts at: 1730 1799 2409 1730
Size: 69 610 4705
Fragments arranged by size:
4705 610 69
BanI G'GyrC_C
Cuts at: 2400 2814 3062 3097 2400
Size: 414 248 35 4687
Fragments arranged by size:
4687 414 248 35
BanII G_rGCy'C
Cuts at: 989 1163 1195 1493 1949 2219 2398 3428 989
Size: 174 32 298 456 270 179 1030 2945
Fragments arranged by size:
2945 1030 456 298 270 179 174 32
BbeI G_GCGC'C
Cuts at: 2818 3066 2818
Size: 248 5136
BbsI GAAGACnn'nnnn_
Cuts at: 605 605
Size: 5384
BciVI GTATCCnnnnn_n'
Cuts at: 436 899 3282 4043 5384 436
Size: 463 2383 761 1341 436
Fragments arranged by size:
2383 1341 761 463 436
BclI T'GATC_A
Cuts at: 1997 2904 1997
Size: 907 4477
BglI GCCn_nnn'nGGC
Cuts at: 615 615
Size: 5384
BglII A'GATC_T
Cuts at: 1236 1686 2899 4936 1236
Size: 450 1213 2037 1684
Fragments arranged by size:
2037 1684 1213 450
BlpI GC'TnA_GC
Cuts at: 622 2496 622
Size: 1874 3510
Bme1580I G_kGCm'C
Cuts at: 1646 2219 3009 3102 4158 1646
Size: 573 790 93 1056 2872
Fragments arranged by size:
2872 1056 790 573 93
BmrI ACTGGGnnnn_n'
Cuts at: 1098 1178 1908 2162 2596 2677 3012 5156 1098
Size: 80 730 254 434 81 335 2144 1326
Fragments arranged by size:
2144 1326 730 434 335 254 81 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 3751 2188
Size: 1563 3821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 698 899 1219 1908 2011 123
Size: 575 201 320 689 103 3496
Fragments arranged by size:
3496 689 575 320 201 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1138 2560 3439 3931 4229 1138
Size: 1422 879 492 298 2293
Fragments arranged by size:
2293 1422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 986 2350 5088 986
Size: 1364 2738 1282
Fragments arranged by size:
2738 1364 1282
BsaAI yAC'GTr
Cuts at: 890 2737 890
Size: 1847 3537
BsaBI GATnn'nnATC
Cuts at: 6 2898 4935 6
Size: 2892 2037 455
Fragments arranged by size:
2892 2037 455
BsaHI Gr'CG_yC
Cuts at: 775 2815 3063 3765 5261 775
Size: 2040 248 702 1496 898
Fragments arranged by size:
2040 1496 898 702 248
BsaWI w'CCGG_w
Cuts at: 439 664 1536 1871 2288 2573 3094 3772 4046
Size: 225 872 335 417 285 521 678 274
Cuts at: 4046 4193 4604 439
Size: 147 411 1219
Fragments arranged by size:
1219 872 678 521 417 411 335 285
274 225 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 695 1222 1351 1864 2368 695
Size: 527 129 513 504 3711
Fragments arranged by size:
3711 527 513 504 129
BseYI C'CCAG_C
Cuts at: 1040 1162 1662 2809 3567 4144 4640 1040
Size: 122 500 1147 758 577 496 1784
Fragments arranged by size:
1784 1147 758 577 500 496 122
BsiEI CG_ry'CG
Cuts at: 104 1369 2926 2972 4180 104
Size: 1265 1557 46 1208 1308
Fragments arranged by size:
1557 1308 1265 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1949 2398 3176 3366 4158 1949
Size: 449 778 190 792 3175
Fragments arranged by size:
3175 792 778 449 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2101 2101
Size: 5384
Bsp1286I G_dGCh'C
Cuts at: 989 1163 1195 1493 1646 1949 2219 2398 3009
Size: 174 32 298 153 303 270 179 611
Cuts at: 3009 3102 3176 3366 3428 4158 989
Size: 93 74 190 62 730 2215
Fragments arranged by size:
2215 730 611 303 298 270 190 179
174 153 93 74 62 32
BspEI T'CCGG_A
Cuts at: 1536 4604 1536
Size: 3068 2316
BspHI T'CATG_A
Cuts at: 1133 4560 1133
Size: 3427 1957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 683 2287 2368 2434 2648 2950 3331 683
Size: 1604 81 66 214 302 381 2736
Fragments arranged by size:
2736 1604 381 302 214 81 66
BsrBI CCG'CTC
Cuts at: 50 621 2172 3676 3730 5214 5379 50
Size: 571 1551 1504 54 1484 165 55
Fragments arranged by size:
1551 1504 1484 571 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1299 3296 1299
Size: 1997 3387
BsrFI r'CCGG_y
Cuts at: 541 664 3382 3563 3772 541
Size: 123 2718 181 209 2153
Fragments arranged by size:
2718 2153 209 181 123
BssSI C'ACGA_G
Cuts at: 524 3655 4013 524
Size: 3131 358 1895
Fragments arranged by size:
3131 1895 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2761 2761
Size: 5384
BstBI TT'CG_AA
Cuts at: 744 3745 744
Size: 3001 2383
BstYI r'GATC_y
Cuts at: 1 149 456 1236 1686 1730 1799 2409 2876
Size: 148 307 780 450 44 69 610 467
Cuts at: 2876 2899 3234 3480 4492 4578 4590 4936 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 780 610 467 450 449 346 335
307 246 148 86 69 44 23 12
BtgI C'CryG_G
Cuts at: 1404 1611 2348 1404
Size: 207 737 4440
Fragments arranged by size:
4440 737 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3334 3515 3678 3334
Size: 181 163 5040
Fragments arranged by size:
5040 181 163
BtsI GCAGTG_nn'
Cuts at: 253 1027 1177 2627 253
Size: 774 150 1450 3010
Fragments arranged by size:
3010 1450 774 150
DraI TTT'AAA
Cuts at: 2383 2383
Size: 5384
DraIII CAC_nnn'GTG
Cuts at: 1581 2064 1581
Size: 483 4901
DrdI GACnn_nn'nnGTC
Cuts at: 3090 3948 3090
Size: 858 4526
EaeI y'GGCC_r
Cuts at: 498 795 1436 2066 2969 3143 3534 3561 4906
Size: 297 641 630 903 174 391 27 1345
Cuts at: 4906 498
Size: 976
Fragments arranged by size:
1345 976 903 641 630 391 297 174
27
EagI C'GGCC_G
Cuts at: 2969 2969
Size: 5384
EarI CTCTTCn'nnn_
Cuts at: 1498 3407 3617 1498
Size: 1909 210 3265
Fragments arranged by size:
3265 1909 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1602 1812 3592 3902 4048 5194 1602
Size: 210 1780 310 146 1146 1792
Fragments arranged by size:
1792 1780 1146 310 210 146
EcoICRI GAG'CTC
Cuts at: 1947 2396 1947
Size: 449 4935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 183 698 712 899 1219 1264 1908 1987
Size: 60 515 14 187 320 45 644 79
Cuts at: 1987 2011 2074 3209 3641 4388 4656 123
Size: 24 63 1135 432 747 268 851
Fragments arranged by size:
1135 851 747 644 515 432 320 268
187 79 63 60 45 24 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2266 2266
Size: 5384
EcoO109I rG'GnC_Cy
Cuts at: 2215 2216 2523 2215
Size: 1 307 5076
Fragments arranged by size:
5076 307 1
EcoRI G'AATT_C
Cuts at: 958 2388 958
Size: 1430 3954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 210 3403 210
Size: 3193 2191
FspI TGC'GCA
Cuts at: 3165 3165
Size: 5384
HaeII r_GCGC'y
Cuts at: 2818 3066 4088 5067 2818
Size: 248 1022 979 3135
Fragments arranged by size:
3135 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 897 929 1226 1258 1313 1345 1549 1581 1607
Size: 32 297 32 55 32 204 32 26
Cuts at: 1607 1639 1678 1710 2023 2055 3226 3256 3258
Size: 32 39 32 313 32 1171 30 2
Cuts at: 3258 3288 897
Size: 30 2993
Fragments arranged by size:
2993 1171 313 297 204 55 39 32
32 32 32 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 2423 2423
Size: 5384
KasI G'GCGC_C
Cuts at: 2814 3062 2814
Size: 248 5136
KpnI G_GTAC'C
Cuts at: 2404 2404
Size: 5384
MluI A'CGCG_T
Cuts at: 4817 4817
Size: 5384
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4055 4239 4055
Size: 184 5200
MscI TGG'CCA
Cuts at: 500 1438 3145 500
Size: 938 1707 2739
Fragments arranged by size:
2739 1707 938
MslI CAynn'nnrTG
Cuts at: 343 1312 3373 3500 343
Size: 969 2061 127 2227
Fragments arranged by size:
2227 2061 969 127
MspA1I CmG'CkG
Cuts at: 626 738 1173 1659 2495 2809 3169 4182 4427
Size: 112 435 486 836 314 360 1013 245
Cuts at: 4427 626
Size: 1583
Fragments arranged by size:
1583 1013 836 486 435 360 314 245
112
NaeI GCC'GGC
Cuts at: 3565 3565
Size: 5384
NarI GG'CG_CC
Cuts at: 2815 3063 2815
Size: 248 5136
NcoI C'CATG_G
Cuts at: 1611 1611
Size: 5384
NdeI CA'TA_TG
Cuts at: 2307 2307
Size: 5384
NgoMIV G'CCGG_C
Cuts at: 3563 3563
Size: 5384
NspI r_CATG'y
Cuts at: 1524 2437 2937 3844 1524
Size: 913 500 907 3064
Fragments arranged by size:
3064 913 907 500
PciI A'CATG_T
Cuts at: 1520 3840 1520
Size: 2320 3064
PflMI CCAn_nnn'nTGG
Cuts at: 453 507 1445 1581 453
Size: 54 938 136 4256
Fragments arranged by size:
4256 938 136 54
PmeI GTTT'AAAC
Cuts at: 2383 2383
Size: 5384
PmlI CAC'GTG
Cuts at: 890 890
Size: 5384
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 4523
Size: 32 5352
PspOMI G'GGCC_C
Cuts at: 2215 2215
Size: 5384
PstI C_TGCA'G
Cuts at: 565 1808 1931 2144 2431 3116 565
Size: 1243 123 213 287 685 2833
Fragments arranged by size:
2833 1243 685 287 213 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5384
PvuII CAG'CTG
Cuts at: 626 1659 2809 3169 626
Size: 1033 1150 360 2841
Fragments arranged by size:
2841 1150 1033 360
RsrII CG'GwC_CG
Cuts at: 3579 3579
Size: 5384
SacI G_AGCT'C
Cuts at: 1949 2398 1949
Size: 449 4935
SalI G'TCGA_C
Cuts at: 2421 2421
Size: 5384
SapI GCTCTTCn'nnn_
Cuts at: 1498 3407 3617 1498
Size: 1909 210 3265
Fragments arranged by size:
3265 1909 210
SbfI CC_TGCA'GG
Cuts at: 2431 2431
Size: 5384
ScaI AGT'ACT
Cuts at: 4901 4901
Size: 5384
SfcI C'TryA_G
Cuts at: 33 561 769 1804 1927 2140 2201 2427 3112
Size: 528 208 1035 123 213 61 226 685
Cuts at: 3112 4105 4296 33
Size: 993 191 1121
Fragments arranged by size:
1121 1035 993 685 528 226 213 208
191 123 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 615 615
Size: 5384
SfoI GGC'GCC
Cuts at: 2816 3064 2816
Size: 248 5136
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5384
SgrAI Cr'CCGG_yG
Cuts at: 664 664
Size: 5384
SmaI CCC'GGG
Cuts at: 120 2406 120
Size: 2286 3098
SmlI C'TyrA_G
Cuts at: 1153 2539 3454 3946 4208 1153
Size: 1386 915 492 262 2329
Fragments arranged by size:
2329 1386 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5384
SphI G_CATG'C
Cuts at: 2437 2937 2437
Size: 500 4884
SspI AAT'ATT
Cuts at: 1515 1515
Size: 5384
StyI C'CwwG_G
Cuts at: 1117 1611 2175 2518 2872 1117
Size: 494 564 343 354 3629
Fragments arranged by size:
3629 564 494 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 2967 3100 3559 3768 1383
Size: 1584 133 459 209 2999
Fragments arranged by size:
2999 1584 459 209 133
TatI w'GTAC_w
Cuts at: 1054 1755 4899 1054
Size: 701 3144 1539
Fragments arranged by size:
3144 1539 701
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 4901 4933 274
Size: 32 4595 32 725
Fragments arranged by size:
4595 725 32 32
Tth111I GACn'n_nGTC
Cuts at: 2154 3181 2154
Size: 1027 4357
XbaI T'CTAG_A
Cuts at: 2415 2415
Size: 5384
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2879 2879
Size: 5384
XmaI C'CCGG_G
Cuts at: 118 2404 118
Size: 2286 3098
XmnI GAAnn'nnTTC
Cuts at: 193 962 1936 193
Size: 769 974 3641
Fragments arranged by size:
3641 974 769
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SnaBI
SrfI StuI SwaI XhoI ZraI