Restriction map for
pF1KA1628
(Circular) (Six-base) MAPSORT of: pF1KA1628.seq Check: 7457 from: 1 to: 6863
pF1KA1628 6863 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 374 1770 374
Size: 1396 5467
AccI GT'mk_AC
Cuts at: 3901 3901
Size: 6863
Acc65I G'GTAC_C
Cuts at: 3879 3879
Size: 6863
AclI AA'CG_TT
Cuts at: 6703 6703
Size: 6863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 756 1864 2451 2808 3248 3281 3521 4688
Size: 74 1108 587 357 440 33 240 1167
Cuts at: 4688 5120 5867 6135 682
Size: 432 747 268 1410
Fragments arranged by size:
1410 1167 1108 747 587 440 432 357
268 240 74 33
AfeI AGC'GCT
Cuts at: 1202 2626 1202
Size: 1424 5439
AflIII A'CryG_T
Cuts at: 4215 5319 6296 4215
Size: 1104 977 4782
Fragments arranged by size:
4782 1104 977
AgeI A'CCGG_T
Cuts at: 5251 5251
Size: 6863
AleI CACnn'nnGTG
Cuts at: 313 868 871 2890 313
Size: 555 3 2019 4286
Fragments arranged by size:
4286 2019 555 3
AlwNI CAG_nnn'CTG
Cuts at: 433 705 1087 2474 3414 3429 4168 5735 433
Size: 272 382 1387 940 15 739 1567 1561
Fragments arranged by size:
1567 1561 1387 940 739 382 272 15
ApaI G_GGCC'C
Cuts at: 253 1941 3384 253
Size: 1688 1443 3732
Fragments arranged by size:
3732 1688 1443
ApaLI G'TGCA_C
Cuts at: 1666 3172 3232 5633 1666
Size: 1506 60 2401 2896
Fragments arranged by size:
2896 2401 1506 60
ApoI r'AATT_y
Cuts at: 3867 6216 6472 3867
Size: 2349 256 4258
Fragments arranged by size:
4258 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6863
AvaI C'yCGr_G
Cuts at: 1129 1462 3094 3377 3609 3682 3883 1129
Size: 333 1632 283 232 73 201 4109
Fragments arranged by size:
4109 1632 333 283 232 201 73
BamHI G'GATC_C
Cuts at: 3621 3888 3621
Size: 267 6596
BanI G'GyrC_C
Cuts at: 1125 1224 2319 2325 2711 3879 4293 4541 4576
Size: 99 1095 6 386 1168 414 248 35
Cuts at: 4576 1125
Size: 3412
Fragments arranged by size:
3412 1168 1095 414 386 248 99 35
6
BanII G_rGCy'C
Cuts at: 143 253 1094 1941 2483 2680 2729 3123 3300
Size: 110 841 847 542 197 49 394 177
Cuts at: 3300 3384 3877 4907 143
Size: 84 493 1030 2099
Fragments arranged by size:
2099 1030 847 841 542 493 394 197
177 110 84 49
BbeI G_GCGC'C
Cuts at: 1129 1228 2323 4297 4545 1129
Size: 99 1095 1974 248 3447
Fragments arranged by size:
3447 1974 1095 248 99
BbsI GAAGACnn'nnnn_
Cuts at: 2531 2531
Size: 6863
BbvCI CC'TCA_GC
Cuts at: 909 2117 2831 909
Size: 1208 714 4941
Fragments arranged by size:
4941 1208 714
BciVI GTATCCnnnnn_n'
Cuts at: 4761 5522 6863 4761
Size: 761 1341 4761
Fragments arranged by size:
4761 1341 761
BclI T'GATC_A
Cuts at: 2927 4383 2927
Size: 1456 5407
BfrBI ATG'CAT
Cuts at: 1956 1956
Size: 6863
BglI GCCn_nnn'nGGC
Cuts at: 1233 1242 2187 2643 1233
Size: 9 945 456 5453
Fragments arranged by size:
5453 945 456 9
BglII A'GATC_T
Cuts at: 2078 3287 4378 6415 2078
Size: 1209 1091 2037 2526
Fragments arranged by size:
2526 2037 1209 1091
BlpI GC'TnA_GC
Cuts at: 214 1088 3313 3975 214
Size: 874 2225 662 3102
Fragments arranged by size:
3102 2225 874 662
Bme1580I G_kGCm'C
Cuts at: 253 1670 1941 2716 3176 3236 3384 4488 4581
Size: 1417 271 775 460 60 148 1104 93
Cuts at: 4581 5637 253
Size: 1056 1479
Fragments arranged by size:
1479 1417 1104 1056 775 460 271 148
93 60
BmgBI CAC'GTC
Cuts at: 837 2373 3213 837
Size: 1536 840 4487
Fragments arranged by size:
4487 1536 840
BmrI ACTGGGnnnn_n'
Cuts at: 1682 1941 1964 3413 4075 4156 4491 6635 1682
Size: 259 23 1449 662 81 335 2144 1910
Fragments arranged by size:
2144 1910 1449 662 335 259 81 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2873 5230 2873
Size: 2357 4506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 367 2348 2540 2866 2920 3099 3572 3586
Size: 25 1981 192 326 54 179 473 14
Cuts at: 3586 3730 3797 342
Size: 144 67 3408
Fragments arranged by size:
3408 1981 473 326 192 179 144 67
54 25 14
Bpu10I CC'TnA_GC
Cuts at: 909 1768 2117 2482 2783 2831 909
Size: 859 349 365 301 48 4941
Fragments arranged by size:
4941 859 365 349 301 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 4039 4918 5410 5708 2176
Size: 1863 879 492 298 3331
Fragments arranged by size:
3331 1863 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2153 3763 3849 6567 2153
Size: 1610 86 2718 2449
Fragments arranged by size:
2718 2449 1610 86
BsaAI yAC'GTr
Cuts at: 1614 4216 1614
Size: 2602 4261
BsaBI GATnn'nnATC
Cuts at: 6 4377 6414 6
Size: 4371 2037 455
Fragments arranged by size:
4371 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 692 817 1031 1126 1225 1304 1966 2320
Size: 570 125 214 95 99 79 662 354
Cuts at: 2320 3440 4294 4542 5244 6740 122
Size: 1120 854 248 702 1496 245
Fragments arranged by size:
1496 1120 854 702 662 570 354 248
245 214 125 99 95 79
BsaWI w'CCGG_w
Cuts at: 4052 4573 5251 5525 5672 6083 4052
Size: 521 678 274 147 411 4832
Fragments arranged by size:
4832 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 172 202 2632 2662 2932 2962 172
Size: 30 2430 30 270 30 4073
Fragments arranged by size:
4073 2430 270 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 586 676 2134 2137 2998 3781 3837 3840
Size: 453 90 1458 3 861 783 56 3
Cuts at: 3840 133
Size: 3156
Fragments arranged by size:
3156 1458 861 783 453 90 56 3
3
BseYI C'CCAG_C
Cuts at: 395 1393 1628 1632 1656 1700 1762 2222 3136
Size: 998 235 4 24 44 62 460 914
Cuts at: 3136 3144 3317 3474 4288 5046 5623 6119 395
Size: 8 173 157 814 758 577 496 1139
Fragments arranged by size:
1139 998 914 814 758 577 496 460
235 173 157 62 44 24 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1198 1712 2041 2596 2689 532
Size: 666 514 329 555 93 4706
Fragments arranged by size:
4706 666 555 514 329 93
BsiEI CG_ry'CG
Cuts at: 104 129 135 2139 3367 4405 4451 5659 104
Size: 25 6 2004 1228 1038 46 1208 1308
Fragments arranged by size:
2004 1308 1228 1208 1038 46 25 6
BsiHKAI G_wGCw'C
Cuts at: 353 1094 1159 1670 2789 3123 3176 3236 3877
Size: 741 65 511 1119 334 53 60 641
Cuts at: 3877 4655 4845 5637 353
Size: 778 190 792 1579
Fragments arranged by size:
1579 1119 792 778 741 641 511 334
190 65 60 53
BsmI GAATG_Cn'
Cuts at: 2493 2493
Size: 6863
BsmBI CGTCTCn'nnnn_
Cuts at: 193 193
Size: 6863
Bsp1286I G_dGCh'C
Cuts at: 143 253 353 1094 1159 1670 1941 2483 2680
Size: 110 100 741 65 511 271 542 197
Cuts at: 2680 2716 2729 2789 3123 3176 3236 3300 3384
Size: 36 13 60 334 53 60 64 84
Cuts at: 3384 3877 4488 4581 4655 4845 4907 5637 143
Size: 493 611 93 74 190 62 730 1369
Fragments arranged by size:
1369 741 730 611 542 511 493 334
271 197 190 110 100 93 84 74
65 64 62 60 60 53 36 13
BspEI T'CCGG_A
Cuts at: 6083 6083
Size: 6863
BspHI T'CATG_A
Cuts at: 6039 6039
Size: 6863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 1770 2675 3709 3913 4127 4429 4810 374
Size: 1396 905 1034 204 214 302 381 2427
Fragments arranged by size:
2427 1396 1034 905 381 302 214 204
BsrBI CCG'CTC
Cuts at: 50 1458 5155 5209 6693 6858 50
Size: 1408 3697 54 1484 165 55
Fragments arranged by size:
3697 1484 1408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 652 1779 3459 4775 652
Size: 1127 1680 1316 2740
Fragments arranged by size:
2740 1680 1316 1127
BsrFI r'CCGG_y
Cuts at: 124 1306 1927 2237 2322 2642 3366 4861 5042
Size: 1182 621 310 85 320 724 1495 181
Cuts at: 5042 5251 124
Size: 209 1736
Fragments arranged by size:
1736 1495 1182 724 621 320 310 209
181 85
BsrGI T'GTAC_A
Cuts at: 2759 2759
Size: 6863
BssHII G'CGCG_C
Cuts at: 172 1162 2881 172
Size: 990 1719 4154
Fragments arranged by size:
4154 1719 990
BssSI C'ACGA_G
Cuts at: 2257 5134 5492 2257
Size: 2877 358 3628
Fragments arranged by size:
3628 2877 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3721 4240 3721
Size: 519 6344
BstBI TT'CG_AA
Cuts at: 5224 5224
Size: 6863
BstEII G'GTnAC_C
Cuts at: 315 2499 315
Size: 2184 4679
BstXI CCAn_nnnn'nTGG
Cuts at: 2083 2083
Size: 6863
BstYI r'GATC_y
Cuts at: 1 701 2078 3287 3621 3835 3888 4355 4378
Size: 700 1377 1209 334 214 53 467 23
Cuts at: 4378 4713 4959 5971 6057 6069 6415 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1377 1209 1012 700 467 449 346 335
334 246 214 86 53 23 12
Bsu36I CC'TnA_GG
Cuts at: 434 659 3572 434
Size: 225 2913 3725
Fragments arranged by size:
3725 2913 225
BtgI C'CryG_G
Cuts at: 107 129 135 477 1192 1370 2154 2693 2838
Size: 22 6 342 715 178 784 539 145
Cuts at: 2838 107
Size: 4132
Fragments arranged by size:
4132 784 715 539 342 178 145 22
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4813 4994 5157 4813
Size: 181 163 6519
Fragments arranged by size:
6519 181 163
BtsI GCAGTG_nn'
Cuts at: 232 272 423 1076 1412 1553 2300 2579 4106
Size: 40 151 653 336 141 747 279 1527
Cuts at: 4106 232
Size: 2989
Fragments arranged by size:
2989 1527 747 653 336 279 151 141
40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 909 944 909
Size: 35 6828
DraI TTT'AAA
Cuts at: 3862 3862
Size: 6863
DraIII CAC_nnn'GTG
Cuts at: 2589 2589
Size: 6863
DrdI GACnn_nn'nnGTC
Cuts at: 427 4569 5427 427
Size: 4142 858 1863
Fragments arranged by size:
4142 1863 858
EaeI y'GGCC_r
Cuts at: 126 132 167 497 746 879 1195 1367 2235
Size: 6 35 330 249 133 316 172 868
Cuts at: 2235 3364 4448 4622 5013 5040 6385 126
Size: 1129 1084 174 391 27 1345 604
Fragments arranged by size:
1345 1129 1084 868 604 391 330 316
249 174 172 133 35 27 6
EagI C'GGCC_G
Cuts at: 126 132 3364 4448 126
Size: 6 3232 1084 2541
Fragments arranged by size:
3232 2541 1084 6
EarI CTCTTCn'nnn_
Cuts at: 4886 5096 4886
Size: 210 6653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5071 5381 5527 6673 5071
Size: 310 146 1146 5261
Fragments arranged by size:
5261 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1092 3121 3875 1092
Size: 2029 754 4080
Fragments arranged by size:
4080 2029 754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 367 682 756 1864 2348 2451 2540 2808
Size: 25 315 74 1108 484 103 89 268
Cuts at: 2808 2866 2920 3099 3248 3281 3521 3572 3586
Size: 58 54 179 149 33 240 51 14
Cuts at: 3586 3730 3797 4688 5120 5867 6135 342
Size: 144 67 891 432 747 268 1070
Fragments arranged by size:
1108 1070 891 747 484 432 315 268
268 240 179 149 144 103 89 74
67 58 54 51 33 25 14
EcoO109I rG'GnC_Cy
Cuts at: 730 783 809 1581 1937 1938 2179 2896 3358
Size: 53 26 772 356 1 241 717 462
Cuts at: 3358 3381 3576 4002 730
Size: 23 195 426 3591
Fragments arranged by size:
3591 772 717 462 426 356 241 195
53 26 23 1
EcoRI G'AATT_C
Cuts at: 3867 3867
Size: 6863
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4882 4882
Size: 6863
FspI TGC'GCA
Cuts at: 4644 4644
Size: 6863
HaeII r_GCGC'y
Cuts at: 1129 1138 1204 1228 1400 2323 2628 4297 4545
Size: 9 66 24 172 923 305 1669 248
Cuts at: 4545 5567 6546 1129
Size: 1022 979 1446
Fragments arranged by size:
1669 1446 1022 979 923 305 248 172
66 24 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1123 1155 2513 2545 2632 2664 2932 2953 2964
Size: 32 1358 32 87 32 268 21 11
Cuts at: 2964 2985 3592 3624 3827 3859 4705 4735 4737
Size: 21 607 32 203 32 846 30 2
Cuts at: 4737 4767 1123
Size: 30 3219
Fragments arranged by size:
3219 1358 846 607 268 203 87 32
32 32 32 32 30 30 21 21
11 2
HincII GTy'rAC
Cuts at: 152 2451 3902 152
Size: 2299 1451 3113
Fragments arranged by size:
3113 2299 1451
HindIII A'AGCT_T
Cuts at: 520 520
Size: 6863
KasI G'GCGC_C
Cuts at: 1125 1224 2319 4293 4541 1125
Size: 99 1095 1974 248 3447
Fragments arranged by size:
3447 1974 1095 248 99
KpnI G_GTAC'C
Cuts at: 3883 3883
Size: 6863
MluI A'CGCG_T
Cuts at: 6296 6296
Size: 6863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 966 2687 5534 5718 966
Size: 1721 2847 184 2111
Fragments arranged by size:
2847 2111 1721 184
MscI TGG'CCA
Cuts at: 499 748 881 4624 499
Size: 249 133 3743 2738
Fragments arranged by size:
3743 2738 249 133
MslI CAynn'nnrTG
Cuts at: 313 868 871 1738 2279 2375 2890 2986 4852
Size: 555 3 867 541 96 515 96 1866
Cuts at: 4852 4979 313
Size: 127 2197
Fragments arranged by size:
2197 1866 867 555 541 515 127 96
96 3
MspA1I CmG'CkG
Cuts at: 131 298 740 913 1087 1194 1230 1438 1632
Size: 167 442 173 174 107 36 208 194
Cuts at: 1632 3148 3974 4288 4648 5661 5906 131
Size: 1516 826 314 360 1013 245 1088
Fragments arranged by size:
1516 1088 1013 826 442 360 314 245
208 194 174 173 167 107 36
NaeI GCC'GGC
Cuts at: 126 1308 1929 2239 2324 3368 5044 126
Size: 1182 621 310 85 1044 1676 1945
Fragments arranged by size:
1945 1676 1182 1044 621 310 85
NarI GG'CG_CC
Cuts at: 1126 1225 2320 4294 4542 1126
Size: 99 1095 1974 248 3447
Fragments arranged by size:
3447 1974 1095 248 99
NcoI C'CATG_G
Cuts at: 107 2838 107
Size: 2731 4132
NgoMIV G'CCGG_C
Cuts at: 124 1306 1927 2237 2322 3366 5042 124
Size: 1182 621 310 85 1044 1676 1945
Fragments arranged by size:
1945 1676 1182 1044 621 310 85
NsiI A_TGCA'T
Cuts at: 1958 1958
Size: 6863
NspI r_CATG'y
Cuts at: 2337 2967 3030 3916 4416 5323 2337
Size: 630 63 886 500 907 3877
Fragments arranged by size:
3877 907 886 630 500 63
PasI CC'CwG_GG
Cuts at: 2474 2474
Size: 6863
PciI A'CATG_T
Cuts at: 5319 5319
Size: 6863
PflMI CCAn_nnn'nTGG
Cuts at: 888 1323 2844 3159 3564 888
Size: 435 1521 315 405 4187
Fragments arranged by size:
4187 1521 435 405 315
PfoI T'CCnGG_A
Cuts at: 704 2936 704
Size: 2232 4631
PmeI GTTT'AAAC
Cuts at: 3862 3862
Size: 6863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6002 6034 6002
Size: 32 6831
PpuMI rG'GwC_Cy
Cuts at: 809 1581 2896 3576 809
Size: 772 1315 680 4096
Fragments arranged by size:
4096 1315 772 680
PshAI GACnn'nnGTC
Cuts at: 511 511
Size: 6863
PspOMI G'GGCC_C
Cuts at: 249 1937 3380 249
Size: 1688 1443 3732
Fragments arranged by size:
3732 1688 1443
PstI C_TGCA'G
Cuts at: 267 1083 1301 2010 2921 3425 3484 3910 4595
Size: 816 218 709 911 504 59 426 685
Cuts at: 4595 267
Size: 2535
Fragments arranged by size:
2535 911 816 709 685 504 426 218
59
PvuI CG_AT'CG
Cuts at: 104 2139 104
Size: 2035 4828
PvuII CAG'CTG
Cuts at: 913 1632 3148 4288 4648 913
Size: 719 1516 1140 360 3128
Fragments arranged by size:
3128 1516 1140 719 360
RsrII CG'GwC_CG
Cuts at: 5058 5058
Size: 6863
SacI G_AGCT'C
Cuts at: 1094 3123 3877 1094
Size: 2029 754 4080
Fragments arranged by size:
4080 2029 754
SacII CC_GC'GG
Cuts at: 132 1195 132
Size: 1063 5800
SalI G'TCGA_C
Cuts at: 3900 3900
Size: 6863
SanDI GG'GwC_CC
Cuts at: 809 809
Size: 6863
SapI GCTCTTCn'nnn_
Cuts at: 4886 5096 4886
Size: 210 6653
SbfI CC_TGCA'GG
Cuts at: 1301 2921 3484 3910 1301
Size: 1620 563 426 4254
Fragments arranged by size:
4254 1620 563 426
ScaI AGT'ACT
Cuts at: 6380 6380
Size: 6863
SexAI A'CCwGG_T
Cuts at: 644 3488 644
Size: 2844 4019
SfcI C'TryA_G
Cuts at: 33 263 276 1079 1297 1570 2006 2917 3421
Size: 230 13 803 218 273 436 911 504
Cuts at: 3421 3480 3906 4591 5584 5775 33
Size: 59 426 685 993 191 1121
Fragments arranged by size:
1121 993 911 803 685 504 436 426
273 230 218 191 59 13
SfoI GGC'GCC
Cuts at: 1127 1226 2321 4295 4543 1127
Size: 99 1095 1974 248 3447
Fragments arranged by size:
3447 1974 1095 248 99
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6863
SmaI CCC'GGG
Cuts at: 3096 3379 3684 3885 3096
Size: 283 305 201 6074
Fragments arranged by size:
6074 305 283 201
SmlI C'TyrA_G
Cuts at: 2191 4018 4933 5425 5687 2191
Size: 1827 915 492 262 3367
Fragments arranged by size:
3367 1827 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6863
SphI G_CATG'C
Cuts at: 3916 4416 3916
Size: 500 6363
StuI AGG'CCT
Cuts at: 3074 3827 3074
Size: 753 6110
StyI C'CwwG_G
Cuts at: 107 2838 3997 4351 107
Size: 2731 1159 354 2619
Fragments arranged by size:
2731 2619 1159 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 2089 2980 3235 4446 4579 5038 5247 423
Size: 1666 891 255 1211 133 459 209 2039
Fragments arranged by size:
2039 1666 1211 891 459 255 209 133
TatI w'GTAC_w
Cuts at: 1790 2759 3110 6378 1790
Size: 969 351 3268 2275
Fragments arranged by size:
3268 2275 969 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 793 825 3046 3078 6380 6412 793
Size: 32 2221 32 3302 32 1244
Fragments arranged by size:
3302 2221 1244 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 838 2688 4660 838
Size: 1850 1972 3041
Fragments arranged by size:
3041 1972 1850
XbaI T'CTAG_A
Cuts at: 3894 3894
Size: 6863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1205 1264 1659 2230 4358 1205
Size: 59 395 571 2128 3710
Fragments arranged by size:
3710 2128 571 395 59
XmaI C'CCGG_G
Cuts at: 3094 3377 3682 3883 3094
Size: 283 305 201 6074
Fragments arranged by size:
6074 305 283 201
XmnI GAAnn'nnTTC
Cuts at: 2551 2551
Size: 6863
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BmtI BsiWI BstZ17I ClaI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI