Restriction map for
pF1KA1643
(Circular) (Six-base) MAPSORT of: pF1KA1643.seq Check: 1414 from: 1 to: 5771
pF1KA1643 5771 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2679 2679
Size: 5771
AatII G_ACGT'C
Cuts at: 602 602
Size: 5771
AccI GT'mk_AC
Cuts at: 1263 2809 1263
Size: 1546 4225
Acc65I G'GTAC_C
Cuts at: 731 2787 731
Size: 2056 3715
AclI AA'CG_TT
Cuts at: 5611 5611
Size: 5771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3596 4028 4381 5043 3596
Size: 432 353 662 4324
Fragments arranged by size:
4324 662 432 353
AfeI AGC'GCT
Cuts at: 584 584
Size: 5771
AflIII A'CryG_T
Cuts at: 860 887 2723 3123 4923 5204 860
Size: 27 1836 400 1800 281 1427
Fragments arranged by size:
1836 1800 1427 400 281 27
AhdI GACnn_n'nnGTC
Cuts at: 1170 1170
Size: 5771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 179 490 179
Size: 311 5460
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1288 1320 1288
Size: 32 5739
AlwNI CAG_nnn'CTG
Cuts at: 543 924 1128 1391 1538 2142 2439 3076 4514
Size: 381 204 263 147 604 297 637 1438
Cuts at: 4514 543
Size: 1800
Fragments arranged by size:
1800 1438 637 604 381 297 263 204
147
ApaI G_GGCC'C
Cuts at: 720 1923 720
Size: 1203 4568
ApaLI G'TGCA_C
Cuts at: 249 2465 2709 4609 249
Size: 2216 244 1900 1411
Fragments arranged by size:
2216 1900 1411 244
ApoI r'AATT_y
Cuts at: 2775 5124 5380 2775
Size: 2349 256 3166
Fragments arranged by size:
3166 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5771
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5771
AvaI C'yCGr_G
Cuts at: 1351 1492 1863 2791 1351
Size: 141 371 928 4331
Fragments arranged by size:
4331 928 371 141
BamHI G'GATC_C
Cuts at: 150 2796 150
Size: 2646 3125
BanI G'GyrC_C
Cuts at: 415 523 642 731 1023 1528 1879 1941 2130
Size: 108 119 89 292 505 351 62 189
Cuts at: 2130 2547 2787 3201 3449 3484 415
Size: 417 240 414 248 35 2702
Fragments arranged by size:
2702 505 417 414 351 292 248 240
189 119 108 89 62 35
BanII G_rGCy'C
Cuts at: 623 720 1724 1923 1982 2116 2152 2785 3815
Size: 97 1004 199 59 134 36 633 1030
Cuts at: 3815 623
Size: 2579
Fragments arranged by size:
2579 1030 1004 633 199 134 97 59
36
BbeI G_GCGC'C
Cuts at: 527 1027 1945 2134 3205 3453 527
Size: 500 918 189 1071 248 2845
Fragments arranged by size:
2845 1071 918 500 248 189
BbsI GAAGACnn'nnnn_
Cuts at: 863 2537 863
Size: 1674 4097
BbvCI CC'TCA_GC
Cuts at: 586 586
Size: 5771
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2031 2065 2031
Size: 34 5737
BciVI GTATCCnnnnn_n'
Cuts at: 3669 4725 5771 3669
Size: 1056 1046 3669
Fragments arranged by size:
3669 1056 1046
BclI T'GATC_A
Cuts at: 3291 3291
Size: 5771
BglI GCCn_nnn'nGGC
Cuts at: 1526 1868 2233 1526
Size: 342 365 5064
Fragments arranged by size:
5064 365 342
BglII A'GATC_T
Cuts at: 2636 3286 5323 2636
Size: 650 2037 3084
Fragments arranged by size:
3084 2037 650
BlpI GC'TnA_GC
Cuts at: 622 1138 1759 1899 2883 622
Size: 516 621 140 984 3510
Fragments arranged by size:
3510 984 621 516 140
Bme1580I G_kGCm'C
Cuts at: 253 720 1507 1531 1923 2469 2713 3396 3489
Size: 467 787 24 392 546 244 683 93
Cuts at: 3489 4613 253
Size: 1124 1411
Fragments arranged by size:
1411 1124 787 683 546 467 392 244
93 24
BmgBI CAC'GTC
Cuts at: 516 978 1002 1320 1833 516
Size: 462 24 318 513 4454
Fragments arranged by size:
4454 513 462 318 24
BmrI ACTGGGnnnn_n'
Cuts at: 1775 2983 3064 3399 5543 1775
Size: 1208 81 335 2144 2003
Fragments arranged by size:
2144 2003 1208 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1191 1731 4138 1191
Size: 540 2407 2824
Fragments arranged by size:
2824 2407 540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 529 1150 1241 2136 481
Size: 48 621 91 895 4116
Fragments arranged by size:
4116 895 621 91 48
Bpu10I CC'TnA_GC
Cuts at: 586 1070 586
Size: 484 5287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1497 2947 3826 4540 4838 1497
Size: 1450 879 714 298 2430
Fragments arranged by size:
2430 1450 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2473 5475 2473
Size: 3002 2769
BsaAI yAC'GTr
Cuts at: 672 3124 672
Size: 2452 3319
BsaBI GATnn'nnATC
Cuts at: 6 3285 5322 6
Size: 3279 2037 455
Fragments arranged by size:
3279 2037 455
BsaHI Gr'CG_yC
Cuts at: 524 599 1024 1871 1942 2131 3202 3450 4152
Size: 75 425 847 71 189 1071 248 702
Cuts at: 4152 5648 524
Size: 1496 647
Fragments arranged by size:
1496 1071 847 702 647 425 248 189
75 71
BsaWI w'CCGG_w
Cuts at: 1622 2252 2960 3481 4159 4570 4717 4991 1622
Size: 630 708 521 678 411 147 274 2402
Fragments arranged by size:
2402 708 678 630 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1288 1318 2470 2500 1288
Size: 30 1152 30 4559
Fragments arranged by size:
4559 1152 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 388 946 949 1306 1309 1483 1951 2169
Size: 186 558 3 357 3 174 468 218
Cuts at: 2169 2646 202
Size: 477 3327
Fragments arranged by size:
3327 558 477 468 357 218 186 174
3 3
BseYI C'CCAG_C
Cuts at: 242 1209 1359 1574 2062 2107 2167 2208 3196
Size: 967 150 215 488 45 60 41 988
Cuts at: 3196 3954 4619 5027 242
Size: 758 665 408 986
Fragments arranged by size:
988 986 967 758 665 488 408 215
150 60 45 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 925 1024 1546 1878 2556 694
Size: 231 99 522 332 678 3909
Fragments arranged by size:
3909 678 522 332 231 99
BsiEI CG_ry'CG
Cuts at: 104 1697 3313 3359 4589 104
Size: 1593 1616 46 1230 1286
Fragments arranged by size:
1616 1593 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 253 623 695 2469 2713 2785 3563 3753 4613
Size: 370 72 1774 244 72 778 190 860
Cuts at: 4613 253
Size: 1411
Fragments arranged by size:
1774 1411 860 778 370 244 190 72
72
BsiWI C'GTAC_G
Cuts at: 1886 1886
Size: 5771
BsmI GAATG_Cn'
Cuts at: 1788 1788
Size: 5771
BsmBI CGTCTCn'nnnn_
Cuts at: 636 697 1594 636
Size: 61 897 4813
Fragments arranged by size:
4813 897 61
Bsp1286I G_dGCh'C
Cuts at: 253 623 695 720 1507 1531 1724 1923 1982
Size: 370 72 25 787 24 193 199 59
Cuts at: 1982 2116 2152 2469 2713 2785 3396 3489 3563
Size: 134 36 317 244 72 611 93 74
Cuts at: 3563 3753 3815 4613 253
Size: 190 62 798 1411
Fragments arranged by size:
1411 798 787 611 370 317 244 199
193 190 134 93 74 72 72 62
59 36 25 24
BspHI T'CATG_A
Cuts at: 446 4203 446
Size: 3757 2014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2062 2679 2821 3035 3337 3718 2062
Size: 617 142 214 302 381 4115
Fragments arranged by size:
4115 617 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 275 1228 1435 4063 4117 5601 5766 50
Size: 225 953 207 2628 54 1484 165 55
Fragments arranged by size:
2628 1484 953 225 207 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1193 3683 1193
Size: 2490 3281
BsrFI r'CCGG_y
Cuts at: 397 433 765 1894 2697 2758 3769 3950 397
Size: 36 332 1129 803 61 1011 181 2218
Fragments arranged by size:
2218 1129 1011 803 332 181 61 36
BssSI C'ACGA_G
Cuts at: 811 2125 4042 4750 811
Size: 1314 1917 708 1832
Fragments arranged by size:
1917 1832 1314 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2579 2717 3148 2579
Size: 138 431 5202
Fragments arranged by size:
5202 431 138
BstBI TT'CG_AA
Cuts at: 4132 4132
Size: 5771
BstEII G'GTnAC_C
Cuts at: 2427 2427
Size: 5771
BstXI CCAn_nnnn'nTGG
Cuts at: 1376 1683 1376
Size: 307 5464
BstYI r'GATC_y
Cuts at: 1 150 377 2249 2636 2796 3263 3286 3621
Size: 149 227 1872 387 160 467 23 335
Cuts at: 3621 3867 4173 4185 4271 5323 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1872 1052 467 449 387 335 306 246
227 160 149 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 371 1052 2019 2135 371
Size: 681 967 116 4007
Fragments arranged by size:
4007 967 681 116
BtgI C'CryG_G
Cuts at: 206 638 1649 1724 2265 206
Size: 432 1011 75 541 3712
Fragments arranged by size:
3712 1011 541 432 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2531 3721 3902 4065 2531
Size: 1190 181 163 4237
Fragments arranged by size:
4237 1190 181 163
BtsI GCAGTG_nn'
Cuts at: 1290 2616 2715 3014 1290
Size: 1326 99 299 4047
Fragments arranged by size:
4047 1326 299 99
DraI TTT'AAA
Cuts at: 2770 2770
Size: 5771
DraIII CAC_nnn'GTG
Cuts at: 2579 2579
Size: 5771
DrdI GACnn_nn'nnGTC
Cuts at: 1693 3477 4821 1693
Size: 1784 1344 2643
Fragments arranged by size:
2643 1784 1344
EaeI y'GGCC_r
Cuts at: 203 395 399 428 470 485 635 794 1343
Size: 192 4 29 42 15 150 159 549
Cuts at: 1343 1727 2384 3356 3530 3921 3948 5293 203
Size: 384 657 972 174 391 27 1345 681
Fragments arranged by size:
1345 972 681 657 549 391 384 192
174 159 150 42 29 27 15 4
EagI C'GGCC_G
Cuts at: 3356 3356
Size: 5771
EarI CTCTTCn'nnn_
Cuts at: 700 3794 4004 700
Size: 3094 210 2467
Fragments arranged by size:
3094 2467 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 246 1445 2593 3979 4721 4867 5581 246
Size: 1199 1148 1386 742 146 714 436
Fragments arranged by size:
1386 1199 1148 742 714 436 146
EcoICRI GAG'CTC
Cuts at: 621 2783 621
Size: 2162 3609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 529 1150 1241 2136 3596 4028 4381 5043
Size: 48 621 91 895 1460 432 353 662
Cuts at: 5043 481
Size: 1209
Fragments arranged by size:
1460 1209 895 662 621 432 353 91
48
EcoNI CCTnn'n_nnAGG
Cuts at: 739 739
Size: 5771
EcoO109I rG'GnC_Cy
Cuts at: 237 549 716 717 1009 1919 1920 2139 2171
Size: 312 167 1 292 910 1 219 32
Cuts at: 2171 2910 237
Size: 739 3098
Fragments arranged by size:
3098 910 739 312 292 219 167 32
1 1
EcoRI G'AATT_C
Cuts at: 2775 2775
Size: 5771
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3790 3790
Size: 5771
FseI GG_CCGG'CC
Cuts at: 401 2762 401
Size: 2361 3410
FspI TGC'GCA
Cuts at: 3552 3552
Size: 5771
HaeII r_GCGC'y
Cuts at: 527 544 586 1027 1945 2134 3205 3453 4683
Size: 17 42 441 918 189 1071 248 1230
Cuts at: 4683 5454 527
Size: 771 844
Fragments arranged by size:
1230 1071 918 844 771 441 248 189
42 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 1156 1188 2234 2266 2470 2502 3613
Size: 32 989 32 1046 32 204 32 1111
Cuts at: 3613 3643 3645 3675 135
Size: 30 2 30 2231
Fragments arranged by size:
2231 1111 1046 989 204 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2810 2810
Size: 5771
HindIII A'AGCT_T
Cuts at: 1756 1756
Size: 5771
KasI G'GCGC_C
Cuts at: 523 1023 1941 2130 3201 3449 523
Size: 500 918 189 1071 248 2845
Fragments arranged by size:
2845 1071 918 500 248 189
KpnI G_GTAC'C
Cuts at: 735 2791 735
Size: 2056 3715
MluI A'CGCG_T
Cuts at: 5204 5204
Size: 5771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 128 1991 2021 4530 4714 128
Size: 1863 30 2509 184 1185
Fragments arranged by size:
2509 1863 1185 184 30
MscI TGG'CCA
Cuts at: 472 487 796 1345 2386 3532 472
Size: 15 309 549 1041 1146 2711
Fragments arranged by size:
2711 1146 1041 549 309 15
MslI CAynn'nnrTG
Cuts at: 445 472 1374 3760 3887 445
Size: 27 902 2386 127 2329
Fragments arranged by size:
2386 2329 902 127 27
MspA1I CmG'CkG
Cuts at: 159 208 246 405 483 1015 1363 1544 2167
Size: 49 38 159 78 532 348 181 623
Cuts at: 2167 2325 2627 2673 2882 3196 3556 4340 4585
Size: 158 302 46 209 314 360 784 245
Cuts at: 4585 159
Size: 1345
Fragments arranged by size:
1345 784 623 532 360 348 314 302
245 209 181 159 158 78 49 46
38
NaeI GCC'GGC
Cuts at: 399 435 767 1896 2760 3952 399
Size: 36 332 1129 864 1192 2218
Fragments arranged by size:
2218 1192 1129 864 332 36
NarI GG'CG_CC
Cuts at: 524 1024 1942 2131 3202 3450 524
Size: 500 918 189 1071 248 2845
Fragments arranged by size:
2845 1071 918 500 248 189
NcoI C'CATG_G
Cuts at: 638 638
Size: 5771
NgoMIV G'CCGG_C
Cuts at: 397 433 765 1894 2758 3950 397
Size: 36 332 1129 864 1192 2218
Fragments arranged by size:
2218 1192 1129 864 332 36
NspI r_CATG'y
Cuts at: 864 2107 2224 2727 2824 3324 4927 864
Size: 1243 117 503 97 500 1603 1708
Fragments arranged by size:
1708 1603 1243 503 500 117 97
PasI CC'CwG_GG
Cuts at: 659 659
Size: 5771
PciI A'CATG_T
Cuts at: 860 2723 4923 860
Size: 1863 2200 1708
Fragments arranged by size:
2200 1863 1708
PflMI CCAn_nnn'nTGG
Cuts at: 315 978 1128 315
Size: 663 150 4958
Fragments arranged by size:
4958 663 150
PmeI GTTT'AAAC
Cuts at: 2770 2770
Size: 5771
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 944 976 1288 1320 4217 4249 944
Size: 32 312 32 2897 32 2466
Fragments arranged by size:
2897 2466 312 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 237 549 1009 2171 237
Size: 312 460 1162 3837
Fragments arranged by size:
3837 1162 460 312
PspOMI G'GGCC_C
Cuts at: 716 1919 716
Size: 1203 4568
PstI C_TGCA'G
Cuts at: 920 1543 2330 2818 3503 920
Size: 623 787 488 685 3188
Fragments arranged by size:
3188 787 685 623 488
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5771
PvuII CAG'CTG
Cuts at: 159 405 1363 1544 2167 2325 2627 3196 3556
Size: 246 958 181 623 158 302 569 360
Cuts at: 3556 159
Size: 2374
Fragments arranged by size:
2374 958 623 569 360 302 246 181
158
RsrII CG'GwC_CG
Cuts at: 2700 3966 2700
Size: 1266 4505
SacI G_AGCT'C
Cuts at: 623 2785 623
Size: 2162 3609
SacII CC_GC'GG
Cuts at: 209 209
Size: 5771
SalI G'TCGA_C
Cuts at: 2808 2808
Size: 5771
SanDI GG'GwC_CC
Cuts at: 1009 1009
Size: 5771
SapI GCTCTTCn'nnn_
Cuts at: 700 3794 4004 700
Size: 3094 210 2467
Fragments arranged by size:
3094 2467 210
SbfI CC_TGCA'GG
Cuts at: 2818 2818
Size: 5771
ScaI AGT'ACT
Cuts at: 667 5288 667
Size: 4621 1150
SexAI A'CCwGG_T
Cuts at: 197 197
Size: 5771
SfcI C'TryA_G
Cuts at: 33 916 1539 2326 2745 2814 3499 4467 4658
Size: 883 623 787 419 69 685 968 191
Cuts at: 4658 33
Size: 1146
Fragments arranged by size:
1146 968 883 787 685 623 419 191
69
SfoI GGC'GCC
Cuts at: 525 1025 1943 2132 3203 3451 525
Size: 500 918 189 1071 248 2845
Fragments arranged by size:
2845 1071 918 500 248 189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5771
SmaI CCC'GGG
Cuts at: 1865 2793 1865
Size: 928 4843
SmlI C'TyrA_G
Cuts at: 1476 1492 2926 3841 4555 4817 1476
Size: 16 1434 915 714 262 2430
Fragments arranged by size:
2430 1434 915 714 262 16
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5771
SphI G_CATG'C
Cuts at: 2107 2224 2824 3324 2107
Size: 117 600 500 4554
Fragments arranged by size:
4554 600 500 117
StuI AGG'CCT
Cuts at: 1252 2560 1252
Size: 1308 4463
StyI C'CwwG_G
Cuts at: 488 638 1904 2453 2905 3259 488
Size: 150 1266 549 452 354 3000
Fragments arranged by size:
3000 1266 549 452 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1364 1573 1717 2446 2535 2728 3354 3487 3946
Size: 209 144 729 89 193 626 133 459
Cuts at: 3946 4155 1364
Size: 209 2980
Fragments arranged by size:
2980 729 626 459 209 209 193 144
133 89
TatI w'GTAC_w
Cuts at: 665 5286 665
Size: 4621 1150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5288 5320 5288
Size: 32 5739
Tth111I GACn'n_nGTC
Cuts at: 2484 3568 2484
Size: 1084 4687
XbaI T'CTAG_A
Cuts at: 2802 2802
Size: 5771
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 3266 480
Size: 2786 2985
XhoI C'TCGA_G
Cuts at: 1492 1492
Size: 5771
XmaI C'CCGG_G
Cuts at: 1863 2791 1863
Size: 928 4843
XmnI GAAnn'nnTTC
Cuts at: 115 2638 115
Size: 2523 3248
ZraI GAC'GTC
Cuts at: 600 600
Size: 5771
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AleI AscI AvrII BaeI BfrBI BmtI
BspEI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SfiI SgrAI
SnaBI SrfI SspI SwaI