Restriction map for
pF1KA1645
(Circular) (Six-base) MAPSORT of: pF1KA1645.seq Check: 1360 from: 1 to: 4094
pF1KA1645 4094 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 828 828
Size: 4094
AatII G_ACGT'C
Cuts at: 556 692 556
Size: 136 3958
AccI GT'mk_AC
Cuts at: 1132 1132
Size: 4094
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 3934 3934
Size: 4094
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 589 1919 2351 2704 3366 397
Size: 192 1330 432 353 662 1125
Fragments arranged by size:
1330 1125 662 432 353 192
AfeI AGC'GCT
Cuts at: 414 414
Size: 4094
AflIII A'CryG_T
Cuts at: 1446 3246 3527 1446
Size: 1800 281 2013
Fragments arranged by size:
2013 1800 281
AgeI A'CCGG_T
Cuts at: 171 171
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 336 534 834 969 1067 1399 2837 336
Size: 198 300 135 98 332 1438 1593
Fragments arranged by size:
1593 1438 332 300 198 135 98
ApaLI G'TGCA_C
Cuts at: 2932 2932
Size: 4094
ApoI r'AATT_y
Cuts at: 1098 3447 3703 1098
Size: 2349 256 1489
Fragments arranged by size:
2349 1489 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 868 1114 868
Size: 246 3848
BamHI G'GATC_C
Cuts at: 1119 1119
Size: 4094
BanI G'GyrC_C
Cuts at: 160 215 347 1110 1524 1772 1807 160
Size: 55 132 763 414 248 35 2447
Fragments arranged by size:
2447 763 414 248 132 55 35
BanII G_rGCy'C
Cuts at: 204 737 1045 1108 2138 204
Size: 533 308 63 1030 2160
Fragments arranged by size:
2160 1030 533 308 63
BbeI G_GCGC'C
Cuts at: 219 351 1528 1776 219
Size: 132 1177 248 2537
Fragments arranged by size:
2537 1177 248 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 699 733 699
Size: 34 4060
BciVI GTATCCnnnnn_n'
Cuts at: 1992 3048 4094 1992
Size: 1056 1046 1992
Fragments arranged by size:
1992 1056 1046
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglI GCCn_nnn'nGGC
Cuts at: 112 308 345 979 112
Size: 196 37 634 3227
Fragments arranged by size:
3227 634 196 37
BglII A'GATC_T
Cuts at: 1609 3646 1609
Size: 2037 2057
BlpI GC'TnA_GC
Cuts at: 1206 1206
Size: 4094
Bme1580I G_kGCm'C
Cuts at: 1719 1812 2936 1719
Size: 93 1124 2877
Fragments arranged by size:
2877 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 138 159 1306 1387 1722 3866 138
Size: 21 1147 81 335 2144 366
Fragments arranged by size:
2144 1147 366 335 81 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 2461 561
Size: 1900 2194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 282 556 831 119
Size: 163 274 275 3382
Fragments arranged by size:
3382 275 274 163
Bpu10I CC'TnA_GC
Cuts at: 209 209
Size: 4094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2863 3161 1270
Size: 879 714 298 2203
Fragments arranged by size:
2203 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 242 3798 242
Size: 3556 538
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 216 348 553 689 1525 1773 2475 3971 216
Size: 132 205 136 836 248 702 1496 339
Fragments arranged by size:
1496 836 702 339 248 205 136 132
BsaWI w'CCGG_w
Cuts at: 171 1283 1804 2482 2893 3040 3314 171
Size: 1112 521 678 411 147 274 951
Fragments arranged by size:
1112 951 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 742 772 619
Size: 30 93 30 3941
Fragments arranged by size:
3941 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 346 745 214
Size: 132 399 3563
Fragments arranged by size:
3563 399 132
BseYI C'CCAG_C
Cuts at: 591 923 1519 2277 2942 3350 591
Size: 332 596 758 665 408 1335
Fragments arranged by size:
1335 758 665 596 408 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 729 169
Size: 560 3534
BsiEI CG_ry'CG
Cuts at: 104 1636 1682 2912 104
Size: 1532 46 1230 1286
Fragments arranged by size:
1532 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1108 1886 2076 2936 1108
Size: 778 190 860 2266
Fragments arranged by size:
2266 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 696 696
Size: 4094
Bsp1286I G_dGCh'C
Cuts at: 204 737 1045 1108 1719 1812 1886 2076 2138
Size: 533 308 63 611 93 74 190 62
Cuts at: 2138 2936 204
Size: 798 1362
Fragments arranged by size:
1362 798 611 533 308 190 93 74
63 62
BspHI T'CATG_A
Cuts at: 852 2526 852
Size: 1674 2420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 828 1144 1358 1660 2041 828
Size: 316 214 302 381 2881
Fragments arranged by size:
2881 381 316 302 214
BsrBI CCG'CTC
Cuts at: 50 222 283 2386 2440 3924 4089 50
Size: 172 61 2103 54 1484 165 55
Fragments arranged by size:
2103 1484 172 165 61 55 54
BsrDI GCAATG_nn'
Cuts at: 2006 2006
Size: 4094
BsrFI r'CCGG_y
Cuts at: 171 655 2092 2273 171
Size: 484 1437 181 1992
Fragments arranged by size:
1992 1437 484 181
BssSI C'ACGA_G
Cuts at: 2365 3073 2365
Size: 708 3386
BstAPI GCAn_nnn'nTGC
Cuts at: 183 336 722 834 1471 183
Size: 153 386 112 637 2806
Fragments arranged by size:
2806 637 386 153 112
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstEII G'GTnAC_C
Cuts at: 285 285
Size: 4094
BstYI r'GATC_y
Cuts at: 1 905 1119 1586 1609 1944 2190 2496 2508
Size: 904 214 467 23 335 246 306 12
Cuts at: 2508 2594 3646 1
Size: 86 1052 449
Fragments arranged by size:
1052 904 467 449 335 306 246 214
86 23 12
BtgI C'CryG_G
Cuts at: 303 788 1037 303
Size: 485 249 3360
Fragments arranged by size:
3360 485 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 702 2044 2225 2388 702
Size: 1342 181 163 2408
Fragments arranged by size:
2408 1342 181 163
BtsI GCAGTG_nn'
Cuts at: 1337 1337
Size: 4094
DraI TTT'AAA
Cuts at: 1093 1093
Size: 4094
DrdI GACnn_nn'nnGTC
Cuts at: 1800 3144 1800
Size: 1344 2750
EaeI y'GGCC_r
Cuts at: 300 309 470 653 892 911 971 1034 1679
Size: 9 161 183 239 19 60 63 645
Cuts at: 1679 1853 2244 2271 3616 300
Size: 174 391 27 1345 778
Fragments arranged by size:
1345 778 645 391 239 183 174 161
63 60 27 19 9
EagI C'GGCC_G
Cuts at: 1679 1679
Size: 4094
EarI CTCTTCn'nnn_
Cuts at: 232 2117 2327 232
Size: 1885 210 1999
Fragments arranged by size:
1999 1885 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 969 2302 3044 3190 3904 969
Size: 1333 742 146 714 1159
Fragments arranged by size:
1333 1159 742 714 146
EcoICRI GAG'CTC
Cuts at: 1106 1106
Size: 4094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 282 397 556 589 831 1919 2351 2704
Size: 163 115 159 33 242 1088 432 353
Cuts at: 2704 3366 119
Size: 662 847
Fragments arranged by size:
1088 847 662 432 353 242 163 159
115 33
EcoNI CCTnn'n_nnAGG
Cuts at: 361 400 361
Size: 39 4055
EcoO109I rG'GnC_Cy
Cuts at: 372 393 831 1233 372
Size: 21 438 402 3233
Fragments arranged by size:
3233 438 402 21
EcoRI G'AATT_C
Cuts at: 1098 1098
Size: 4094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2113 2113
Size: 4094
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 219 351 416 802 995 1528 1776 3006 3777
Size: 132 65 386 193 533 248 1230 771
Cuts at: 3777 219
Size: 536
Fragments arranged by size:
1230 771 536 533 386 248 193 132
65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 655 662 687 694 742 774 1936
Size: 32 4 7 25 7 48 32 1162
Cuts at: 1936 1966 1968 1998 619
Size: 30 2 30 2715
Fragments arranged by size:
2715 1162 48 32 32 30 30 25
7 7 4 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 215 347 1524 1772 215
Size: 132 1177 248 2537
Fragments arranged by size:
2537 1177 248 132
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2853 3037 2853
Size: 184 3910
MscI TGG'CCA
Cuts at: 302 913 1855 302
Size: 611 942 2541
Fragments arranged by size:
2541 942 611
MslI CAynn'nnrTG
Cuts at: 601 940 2083 2210 601
Size: 339 1143 127 2485
Fragments arranged by size:
2485 1143 339 127
MspA1I CmG'CkG
Cuts at: 790 997 1039 1056 1205 1519 1879 2663 2908
Size: 207 42 17 149 314 360 784 245
Cuts at: 2908 790
Size: 1976
Fragments arranged by size:
1976 784 360 314 245 207 149 42
17
NaeI GCC'GGC
Cuts at: 657 2275 657
Size: 1618 2476
NarI GG'CG_CC
Cuts at: 216 348 1525 1773 216
Size: 132 1177 248 2537
Fragments arranged by size:
2537 1177 248 132
NgoMIV G'CCGG_C
Cuts at: 655 2273 655
Size: 1618 2476
NspI r_CATG'y
Cuts at: 181 600 1147 1647 3250 181
Size: 419 547 500 1603 1025
Fragments arranged by size:
1603 1025 547 500 419
PasI CC'CwG_GG
Cuts at: 342 342
Size: 4094
PciI A'CATG_T
Cuts at: 3246 3246
Size: 4094
PflMI CCAn_nnn'nTGG
Cuts at: 1008 1008
Size: 4094
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2540 2572 2540
Size: 32 4062
PpuMI rG'GwC_Cy
Cuts at: 372 393 372
Size: 21 4073
PstI C_TGCA'G
Cuts at: 272 332 634 839 1141 1826 272
Size: 60 302 205 302 685 2540
Fragments arranged by size:
2540 685 302 302 205 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 1519 1879 1519
Size: 360 3734
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 1108 1108
Size: 4094
SacII CC_GC'GG
Cuts at: 791 1040 791
Size: 249 3845
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 2117 2327 2117
Size: 210 3884
SbfI CC_TGCA'GG
Cuts at: 839 1141 839
Size: 302 3792
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 268 328 630 835 1137 1822 2790 2981
Size: 235 60 302 205 302 685 968 191
Cuts at: 2981 33
Size: 1146
Fragments arranged by size:
1146 968 685 302 302 235 205 191
60
SfiI GGCCn_nnn'nGGCC
Cuts at: 308 979 308
Size: 671 3423
SfoI GGC'GCC
Cuts at: 217 349 1526 1774 217
Size: 132 1177 248 2537
Fragments arranged by size:
2537 1177 248 132
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SgrAI Cr'CCGG_yG
Cuts at: 171 171
Size: 4094
SmaI CCC'GGG
Cuts at: 870 1116 870
Size: 246 3848
SmlI C'TyrA_G
Cuts at: 1249 2164 2878 3140 1249
Size: 915 714 262 2203
Fragments arranged by size:
2203 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 181 600 1147 1647 181
Size: 419 547 500 2628
Fragments arranged by size:
2628 547 500 419
StyI C'CwwG_G
Cuts at: 1046 1228 1582 1046
Size: 182 354 3558
Fragments arranged by size:
3558 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 178 1677 1810 2269 2478 178
Size: 1499 133 459 209 1794
Fragments arranged by size:
1794 1499 459 209 133
TatI w'GTAC_w
Cuts at: 3609 3609
Size: 4094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 3611 3643 415
Size: 32 3164 32 866
Fragments arranged by size:
3164 866 32 32
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 868 1114 868
Size: 246 3848
ZraI GAC'GTC
Cuts at: 554 690 554
Size: 136 3958
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI