Restriction map for
pF1KA1667
(Circular) (Six-base) MAPSORT of: pF1KA1667.seq Check: 1051 from: 1 to: 5237
pF1KA1667 5237 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1956 2139 1956
Size: 183 5054
AccI GT'mk_AC
Cuts at: 2275 2275
Size: 5237
Acc65I G'GTAC_C
Cuts at: 2253 2253
Size: 5237
AclI AA'CG_TT
Cuts at: 5077 5077
Size: 5237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 649 2160 2230 3062 3494 3847 4509 367
Size: 282 1511 70 832 432 353 662 1095
Fragments arranged by size:
1511 1095 832 662 432 353 282 70
AfeI AGC'GCT
Cuts at: 661 661
Size: 5237
AflIII A'CryG_T
Cuts at: 1278 2589 4389 4670 1278
Size: 1311 1800 281 1845
Fragments arranged by size:
1845 1800 1311 281
AhdI GACnn_n'nnGTC
Cuts at: 706 706
Size: 5237
AleI CACnn'nnGTG
Cuts at: 2077 2077
Size: 5237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1154 1725 1154
Size: 571 4666
AlwNI CAG_nnn'CTG
Cuts at: 243 1618 2124 2205 2542 3980 243
Size: 1375 506 81 337 1438 1500
Fragments arranged by size:
1500 1438 1375 506 337 81
ApaLI G'TGCA_C
Cuts at: 4075 4075
Size: 5237
ApoI r'AATT_y
Cuts at: 542 2023 2241 4590 4846 542
Size: 1481 218 2349 256 933
Fragments arranged by size:
2349 1481 933 256 218
AseI AT'TA_AT
Cuts at: 20 20
Size: 5237
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5237
AvaI C'yCGr_G
Cuts at: 2257 2257
Size: 5237
BamHI G'GATC_C
Cuts at: 2262 2262
Size: 5237
BanI G'GyrC_C
Cuts at: 2127 2170 2253 2667 2915 2950 2127
Size: 43 83 414 248 35 4414
Fragments arranged by size:
4414 414 248 83 43 35
BanII G_rGCy'C
Cuts at: 407 617 1305 1425 1721 2146 2251 3281 407
Size: 210 688 120 296 425 105 1030 2363
Fragments arranged by size:
2363 1030 688 425 296 210 120 105
BbeI G_GCGC'C
Cuts at: 2174 2671 2919 2174
Size: 497 248 4492
Fragments arranged by size:
4492 497 248
BbsI GAAGACnn'nnnn_
Cuts at: 1445 1445
Size: 5237
BbvCI CC'TCA_GC
Cuts at: 1162 1778 1162
Size: 616 4621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1701 1735 1701
Size: 34 5203
BciVI GTATCCnnnnn_n'
Cuts at: 3135 4191 5237 3135
Size: 1056 1046 3135
Fragments arranged by size:
3135 1056 1046
BclI T'GATC_A
Cuts at: 2757 2757
Size: 5237
BfrBI ATG'CAT
Cuts at: 2016 2016
Size: 5237
BglI GCCn_nnn'nGGC
Cuts at: 2074 2186 2074
Size: 112 5125
BglII A'GATC_T
Cuts at: 2752 4789 2752
Size: 2037 3200
BlpI GC'TnA_GC
Cuts at: 1641 2349 1641
Size: 708 4529
Bme1580I G_kGCm'C
Cuts at: 2862 2955 4079 2862
Size: 93 1124 4020
Fragments arranged by size:
4020 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2449 2530 2865 5009 2449
Size: 81 335 2144 2677
Fragments arranged by size:
2677 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1911 3604 1911
Size: 1693 3544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 908 919 1126 1317 1671 1981 302
Size: 606 11 207 191 354 310 3558
Fragments arranged by size:
3558 606 354 310 207 191 11
Bpu10I CC'TnA_GC
Cuts at: 1162 1238 1778 1162
Size: 76 540 4621
Fragments arranged by size:
4621 540 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 3292 4006 4304 2413
Size: 879 714 298 3346
Fragments arranged by size:
3346 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 917 4941 917
Size: 4024 1213
BsaAI yAC'GTr
Cuts at: 2590 2590
Size: 5237
BsaBI GATnn'nnATC
Cuts at: 6 2751 4788 6
Size: 2745 2037 455
Fragments arranged by size:
2745 2037 455
BsaHI Gr'CG_yC
Cuts at: 2171 2668 2916 3618 5114 2171
Size: 497 248 702 1496 2294
Fragments arranged by size:
2294 1496 702 497 248
BsaWI w'CCGG_w
Cuts at: 2426 2947 3625 4036 4183 4457 2426
Size: 521 678 411 147 274 3206
Fragments arranged by size:
3206 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 604 634 604
Size: 30 5207
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 308 1158 1795 308
Size: 850 637 3750
Fragments arranged by size:
3750 850 637
BseYI C'CCAG_C
Cuts at: 387 950 1412 1791 2030 2662 3420 4085 4493
Size: 563 462 379 239 632 758 665 408
Cuts at: 4493 387
Size: 1131
Fragments arranged by size:
1131 758 665 632 563 462 408 379
239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 5237
BsiEI CG_ry'CG
Cuts at: 104 1932 2779 2825 4055 104
Size: 1828 847 46 1230 1286
Fragments arranged by size:
1828 1286 1230 847 46
BsiHKAI G_wGCw'C
Cuts at: 407 517 976 2146 2251 3029 3219 4079 407
Size: 110 459 1170 105 778 190 860 1565
Fragments arranged by size:
1565 1170 860 778 459 190 110 105
BsmI GAATG_Cn'
Cuts at: 641 1633 1934 641
Size: 992 301 3944
Fragments arranged by size:
3944 992 301
BsmBI CGTCTCn'nnnn_
Cuts at: 1864 1864
Size: 5237
Bsp1286I G_dGCh'C
Cuts at: 407 517 617 976 1305 1425 1721 2146 2251
Size: 110 100 359 329 120 296 425 105
Cuts at: 2251 2862 2955 3029 3219 3281 4079 407
Size: 611 93 74 190 62 798 1565
Fragments arranged by size:
1565 798 611 425 359 329 296 190
120 110 105 100 93 74 62
BspHI T'CATG_A
Cuts at: 3669 3669
Size: 5237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 1422 1956 1967 2139 2287 2501 2803 3184
Size: 761 534 11 172 148 214 302 381
Cuts at: 3184 661
Size: 2714
Fragments arranged by size:
2714 761 534 381 302 214 172 148
11
BsrBI CCG'CTC
Cuts at: 50 817 3529 3583 5067 5232 50
Size: 767 2712 54 1484 165 55
Fragments arranged by size:
2712 1484 767 165 55 54
BsrDI GCAATG_nn'
Cuts at: 820 3149 820
Size: 2329 2908
BsrFI r'CCGG_y
Cuts at: 3235 3416 3235
Size: 181 5056
BssSI C'ACGA_G
Cuts at: 144 883 1720 1899 3508 4216 144
Size: 739 837 179 1609 708 1165
Fragments arranged by size:
1609 1165 837 739 708 179
BstAPI GCAn_nnn'nTGC
Cuts at: 2205 2614 2205
Size: 409 4828
BstBI TT'CG_AA
Cuts at: 3598 3598
Size: 5237
BstXI CCAn_nnnn'nTGG
Cuts at: 1341 1341
Size: 5237
BstYI r'GATC_y
Cuts at: 1 1035 1530 2262 2729 2752 3087 3333 3639
Size: 1034 495 732 467 23 335 246 306
Cuts at: 3639 3651 3737 4789 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1034 732 495 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 767 1257 1358 2162 767
Size: 490 101 804 3842
Fragments arranged by size:
3842 804 490 101
BtgI C'CryG_G
Cuts at: 107 1016 1559 2072 107
Size: 909 543 513 3272
Fragments arranged by size:
3272 909 543 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3187 3368 3531 3187
Size: 181 163 4893
Fragments arranged by size:
4893 181 163
BtsI GCAGTG_nn'
Cuts at: 1743 2480 1743
Size: 737 4500
DraI TTT'AAA
Cuts at: 2236 2236
Size: 5237
DraIII CAC_nnn'GTG
Cuts at: 521 2220 521
Size: 1699 3538
DrdI GACnn_nn'nnGTC
Cuts at: 2943 4287 2943
Size: 1344 3893
EaeI y'GGCC_r
Cuts at: 110 1002 1095 1449 1970 2822 2996 3387 3414
Size: 892 93 354 521 852 174 391 27
Cuts at: 3414 4759 110
Size: 1345 588
Fragments arranged by size:
1345 892 852 588 521 391 354 174
93 27
EagI C'GGCC_G
Cuts at: 2822 2822
Size: 5237
EarI CTCTTCn'nnn_
Cuts at: 3260 3470 3260
Size: 210 5027
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3445 4187 4333 5047 3445
Size: 742 146 714 3635
Fragments arranged by size:
3635 742 714 146
EcoICRI GAG'CTC
Cuts at: 405 2144 2249 405
Size: 1739 105 3393
Fragments arranged by size:
3393 1739 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 367 649 908 919 1126 1317 1671 1981
Size: 65 282 259 11 207 191 354 310
Cuts at: 1981 2160 2230 3062 3494 3847 4509 302
Size: 179 70 832 432 353 662 1030
Fragments arranged by size:
1030 832 662 432 354 353 310 282
259 207 191 179 70 65 11
EcoNI CCTnn'n_nnAGG
Cuts at: 788 1177 1579 1963 788
Size: 389 402 384 4062
Fragments arranged by size:
4062 402 389 384
EcoO109I rG'GnC_Cy
Cuts at: 744 1499 1508 2376 744
Size: 755 9 868 3605
Fragments arranged by size:
3605 868 755 9
EcoRI G'AATT_C
Cuts at: 2241 2241
Size: 5237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3256 3256
Size: 5237
FspI TGC'GCA
Cuts at: 3018 3018
Size: 5237
HaeII r_GCGC'y
Cuts at: 663 1331 2174 2671 2919 4149 4920 663
Size: 668 843 497 248 1230 771 980
Fragments arranged by size:
1230 980 843 771 668 497 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 692 724 897 929 1027 1059 1716
Size: 32 539 32 173 32 98 32 657
Cuts at: 1716 1748 3079 3109 3111 3141 121
Size: 32 1331 30 2 30 2217
Fragments arranged by size:
2217 1331 657 539 173 98 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2276 2276
Size: 5237
HindIII A'AGCT_T
Cuts at: 1055 1552 1055
Size: 497 4740
KasI G'GCGC_C
Cuts at: 2170 2667 2915 2170
Size: 497 248 4492
Fragments arranged by size:
4492 497 248
KpnI G_GTAC'C
Cuts at: 2257 2257
Size: 5237
MluI A'CGCG_T
Cuts at: 4670 4670
Size: 5237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 226 426 3996 4180 226
Size: 200 3570 184 1283
Fragments arranged by size:
3570 1283 200 184
MscI TGG'CCA
Cuts at: 112 1004 1097 1451 1972 2998 112
Size: 892 93 354 521 1026 2351
Fragments arranged by size:
2351 1026 892 521 354 93
MslI CAynn'nnrTG
Cuts at: 2077 3226 3353 2077
Size: 1149 127 3961
Fragments arranged by size:
3961 1149 127
MspA1I CmG'CkG
Cuts at: 293 419 1788 2034 2124 2348 2662 3022 3806
Size: 126 1369 246 90 224 314 360 784
Cuts at: 3806 4051 293
Size: 245 1479
Fragments arranged by size:
1479 1369 784 360 314 246 245 224
126 90
NaeI GCC'GGC
Cuts at: 3418 3418
Size: 5237
NarI GG'CG_CC
Cuts at: 2171 2668 2916 2171
Size: 497 248 4492
Fragments arranged by size:
4492 497 248
NcoI C'CATG_G
Cuts at: 107 1016 107
Size: 909 4328
NgoMIV G'CCGG_C
Cuts at: 3416 3416
Size: 5237
NsiI A_TGCA'T
Cuts at: 2018 2018
Size: 5237
NspI r_CATG'y
Cuts at: 1282 2290 2790 4393 1282
Size: 1008 500 1603 2126
Fragments arranged by size:
2126 1603 1008 500
PasI CC'CwG_GG
Cuts at: 1173 1880 1173
Size: 707 4530
PciI A'CATG_T
Cuts at: 1278 4389 1278
Size: 3111 2126
PflMI CCAn_nnn'nTGG
Cuts at: 967 1022 1104 2124 967
Size: 55 82 1020 4080
Fragments arranged by size:
4080 1020 82 55
PfoI T'CCnGG_A
Cuts at: 935 1533 2102 935
Size: 598 569 4070
Fragments arranged by size:
4070 598 569
PmeI GTTT'AAAC
Cuts at: 2236 2236
Size: 5237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 750 782 3683 3715 750
Size: 32 2901 32 2272
Fragments arranged by size:
2901 2272 32 32
PpuMI rG'GwC_Cy
Cuts at: 744 1499 744
Size: 755 4482
PstI C_TGCA'G
Cuts at: 650 1674 2136 2284 2969 650
Size: 1024 462 148 685 2918
Fragments arranged by size:
2918 1024 685 462 148
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5237
PvuII CAG'CTG
Cuts at: 419 2034 2124 2662 3022 419
Size: 1615 90 538 360 2634
Fragments arranged by size:
2634 1615 538 360 90
RsrII CG'GwC_CG
Cuts at: 3432 3432
Size: 5237
SacI G_AGCT'C
Cuts at: 407 2146 2251 407
Size: 1739 105 3393
Fragments arranged by size:
3393 1739 105
SalI G'TCGA_C
Cuts at: 2274 2274
Size: 5237
SapI GCTCTTCn'nnn_
Cuts at: 3260 3470 3260
Size: 210 5027
SbfI CC_TGCA'GG
Cuts at: 2284 2284
Size: 5237
ScaI AGT'ACT
Cuts at: 4754 4754
Size: 5237
SfcI C'TryA_G
Cuts at: 33 646 1670 2132 2280 2965 3933 4124 33
Size: 613 1024 462 148 685 968 191 1146
Fragments arranged by size:
1146 1024 968 685 613 462 191 148
SfoI GGC'GCC
Cuts at: 2172 2669 2917 2172
Size: 497 248 4492
Fragments arranged by size:
4492 497 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5237
SmaI CCC'GGG
Cuts at: 2259 2259
Size: 5237
SmlI C'TyrA_G
Cuts at: 2392 3307 4021 4283 2392
Size: 915 714 262 3346
Fragments arranged by size:
3346 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1185 67
Size: 1118 4119
SphI G_CATG'C
Cuts at: 2290 2790 2290
Size: 500 4737
StuI AGG'CCT
Cuts at: 1574 1574
Size: 5237
StyI C'CwwG_G
Cuts at: 107 179 740 1016 2371 2725 107
Size: 72 561 276 1355 354 2619
Fragments arranged by size:
2619 1355 561 354 276 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 372 730 2820 2953 3412 3621 372
Size: 358 2090 133 459 209 1988
Fragments arranged by size:
2090 1988 459 358 209 133
TatI w'GTAC_w
Cuts at: 4752 4752
Size: 5237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1020 1052 4754 4786 1020
Size: 32 3702 32 1471
Fragments arranged by size:
3702 1471 32 32
Tth111I GACn'n_nGTC
Cuts at: 909 3034 909
Size: 2125 3112
XbaI T'CTAG_A
Cuts at: 2268 2268
Size: 5237
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 964 1005 1615 2732 964
Size: 41 610 1117 3469
Fragments arranged by size:
3469 1117 610 41
XmaI C'CCGG_G
Cuts at: 2257 2257
Size: 5237
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AloI ApaI AscI AvrII BaeI
BmgBI BmtI BsiWI BspEI BsrGI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI