Restriction map for
pF1KA1668
(Circular) (Six-base) MAPSORT of: pF1KA1668.seq Check: 2080 from: 1 to: 5702
pF1KA1668 5702 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1299 1666 2037 2094 1299
Size: 367 371 57 4907
Fragments arranged by size:
4907 371 367 57
AccI GT'mk_AC
Cuts at: 2740 2740
Size: 5702
Acc65I G'GTAC_C
Cuts at: 1744 2718 1744
Size: 974 4728
AclI AA'CG_TT
Cuts at: 5542 5542
Size: 5702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3527 3959 4312 4974 3527
Size: 432 353 662 4255
Fragments arranged by size:
4255 662 432 353
AfeI AGC'GCT
Cuts at: 634 1631 634
Size: 997 4705
AflIII A'CryG_T
Cuts at: 3054 4854 5135 3054
Size: 1800 281 3621
Fragments arranged by size:
3621 1800 281
AleI CACnn'nnGTG
Cuts at: 508 742 1228 508
Size: 234 486 4982
Fragments arranged by size:
4982 486 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1136 1168 1136
Size: 32 5670
AlwNI CAG_nnn'CTG
Cuts at: 901 984 1726 2358 3007 4445 901
Size: 83 742 632 649 1438 2158
Fragments arranged by size:
2158 1438 742 649 632 83
ApaI G_GGCC'C
Cuts at: 775 806 2445 775
Size: 31 1639 4032
Fragments arranged by size:
4032 1639 31
ApaLI G'TGCA_C
Cuts at: 619 4540 619
Size: 3921 1781
ApoI r'AATT_y
Cuts at: 2706 5055 5311 2706
Size: 2349 256 3097
Fragments arranged by size:
3097 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5702
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5702
AvaI C'yCGr_G
Cuts at: 774 959 1281 2444 2722 774
Size: 185 322 1163 278 3754
Fragments arranged by size:
3754 1163 322 278 185
BamHI G'GATC_C
Cuts at: 332 2065 2727 332
Size: 1733 662 3307
Fragments arranged by size:
3307 1733 662
BanI G'GyrC_C
Cuts at: 140 236 571 736 1324 1744 1903 1947 2396
Size: 96 335 165 588 420 159 44 449
Cuts at: 2396 2718 3132 3380 3415 140
Size: 322 414 248 35 2427
Fragments arranged by size:
2427 588 449 420 414 335 322 248
165 159 96 44 35
BanII G_rGCy'C
Cuts at: 545 775 806 1351 1717 1945 2333 2393 2445
Size: 230 31 545 366 228 388 60 52
Cuts at: 2445 2716 3746 545
Size: 271 1030 2501
Fragments arranged by size:
2501 1030 545 388 366 271 230 228
60 52 31
BbeI G_GCGC'C
Cuts at: 1907 3136 3384 1907
Size: 1229 248 4225
Fragments arranged by size:
4225 1229 248
BbsI GAAGACnn'nnnn_
Cuts at: 276 2334 2549 276
Size: 2058 215 3429
Fragments arranged by size:
3429 2058 215
BbvCI CC'TCA_GC
Cuts at: 388 902 388
Size: 514 5188
BciVI GTATCCnnnnn_n'
Cuts at: 2166 3600 4656 5702 2166
Size: 1434 1056 1046 2166
Fragments arranged by size:
2166 1434 1056 1046
BclI T'GATC_A
Cuts at: 2564 3222 2564
Size: 658 5044
BglI GCCn_nnn'nGGC
Cuts at: 2079 2079
Size: 5702
BglII A'GATC_T
Cuts at: 3217 5254 3217
Size: 2037 3665
BlpI GC'TnA_GC
Cuts at: 1633 2814 1633
Size: 1181 4521
Bme1580I G_kGCm'C
Cuts at: 574 623 739 775 806 1709 2445 3327 3420
Size: 49 116 36 31 903 736 882 93
Cuts at: 3420 4544 574
Size: 1124 1732
Fragments arranged by size:
1732 1124 903 882 736 116 93 49
36 31
BmrI ACTGGGnnnn_n'
Cuts at: 405 2914 2995 3330 5474 405
Size: 2509 81 335 2144 633
Fragments arranged by size:
2509 2144 633 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 564 1626 4069 561
Size: 3 1062 2443 2194
Fragments arranged by size:
2443 2194 1062 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 675 867 1059 1384 1406 1711 2221 2242
Size: 184 192 192 325 22 305 510 21
Cuts at: 2242 2380 491
Size: 138 3813
Fragments arranged by size:
3813 510 325 305 192 192 184 138
22 21
Bpu10I CC'TnA_GC
Cuts at: 187 388 530 902 1872 187
Size: 201 142 372 970 4017
Fragments arranged by size:
4017 970 372 201 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2878 3757 4471 4769 1817
Size: 1061 879 714 298 2750
Fragments arranged by size:
2750 1061 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 369 5406 369
Size: 5037 665
BsaAI yAC'GTr
Cuts at: 596 3055 596
Size: 2459 3243
BsaBI GATnn'nnATC
Cuts at: 6 3216 5253 6
Size: 3210 2037 455
Fragments arranged by size:
3210 2037 455
BsaHI Gr'CG_yC
Cuts at: 1904 3133 3381 4083 5579 1904
Size: 1229 248 702 1496 2027
Fragments arranged by size:
2027 1496 1229 702 248
BsaWI w'CCGG_w
Cuts at: 674 2015 2393 2891 3412 4090 4501 4648 4922
Size: 1341 378 498 521 678 411 147 274
Cuts at: 4922 674
Size: 1454
Fragments arranged by size:
1454 1341 678 521 498 411 378 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 1559 1589 1633 1663 349
Size: 30 1180 30 44 30 4388
Fragments arranged by size:
4388 1180 44 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 537 553 745 1345 1405 1408 1411 1414 1707
Size: 16 192 600 60 3 3 3 293
Cuts at: 1707 1770 1782 2164 2239 2392 2446 537
Size: 63 12 382 75 153 54 3793
Fragments arranged by size:
3793 600 382 293 192 153 75 63
60 54 16 12 3 3 3
BseYI C'CCAG_C
Cuts at: 112 327 586 768 877 1047 1340 1432 1555
Size: 215 259 182 109 170 293 92 123
Cuts at: 1555 1880 2040 3127 3885 4550 4958 112
Size: 325 160 1087 758 665 408 856
Fragments arranged by size:
1087 856 758 665 408 325 293 259
215 182 170 160 123 109 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 649 770 1123 1252 1412 1471 1700 214
Size: 435 121 353 129 160 59 229 4216
Fragments arranged by size:
4216 435 353 229 160 129 121 59
BsiEI CG_ry'CG
Cuts at: 104 787 940 3244 3290 4520 104
Size: 683 153 2304 46 1230 1286
Fragments arranged by size:
2304 1286 1230 683 153 46
BsiHKAI G_wGCw'C
Cuts at: 545 623 691 891 1042 1717 2207 2333 2393
Size: 78 68 200 151 675 490 126 60
Cuts at: 2393 2716 3494 3684 4544 545
Size: 323 778 190 860 1703
Fragments arranged by size:
1703 860 778 675 490 323 200 190
151 126 78 68 60
Bsp1286I G_dGCh'C
Cuts at: 545 574 623 691 739 775 806 891 1042
Size: 29 49 68 48 36 31 85 151
Cuts at: 1042 1351 1709 1717 1945 2207 2333 2393 2445
Size: 309 358 8 228 262 126 60 52
Cuts at: 2445 2716 3327 3420 3494 3684 3746 4544 545
Size: 271 611 93 74 190 62 798 1703
Fragments arranged by size:
1703 798 611 358 309 271 262 228
190 151 126 93 85 74 68 62
60 52 49 48 36 31 29 8
BspHI T'CATG_A
Cuts at: 395 4134 395
Size: 3739 1963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 1299 1666 1971 2037 2094 2752 2966 3268
Size: 1042 367 305 66 57 658 214 302
Cuts at: 3268 3649 257
Size: 381 2310
Fragments arranged by size:
2310 1042 658 381 367 305 302 214
66 57
BsrBI CCG'CTC
Cuts at: 50 340 1073 3994 4048 5532 5697 50
Size: 290 733 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 733 290 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3614 3614
Size: 5702
BsrFI r'CCGG_y
Cuts at: 233 239 574 2186 2522 3700 3881 233
Size: 6 335 1612 336 1178 181 2054
Fragments arranged by size:
2054 1612 1178 336 335 181 6
BssHII G'CGCG_C
Cuts at: 125 1548 125
Size: 1423 4279
BssSI C'ACGA_G
Cuts at: 2296 3973 4681 2296
Size: 1677 708 3317
Fragments arranged by size:
3317 1677 708
BstAPI GCAn_nnn'nTGC
Cuts at: 596 618 627 850 1146 3079 596
Size: 22 9 223 296 1933 3219
Fragments arranged by size:
3219 1933 296 223 22 9
BstBI TT'CG_AA
Cuts at: 4063 4063
Size: 5702
BstXI CCAn_nnnn'nTGG
Cuts at: 360 510 1741 360
Size: 150 1231 4321
Fragments arranged by size:
4321 1231 150
BstYI r'GATC_y
Cuts at: 1 176 332 944 2065 2727 3194 3217 3552
Size: 175 156 612 1121 662 467 23 335
Cuts at: 3552 3798 4104 4116 4202 5254 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1121 1052 662 612 467 449 335 306
246 175 156 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 549 728 929 2147 2600 549
Size: 179 201 1218 453 3651
Fragments arranged by size:
3651 1218 453 201 179
BtgI C'CryG_G
Cuts at: 107 118 165 1216 2160 2208 107
Size: 11 47 1051 944 48 3601
Fragments arranged by size:
3601 1051 944 48 47 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 646 1607 3652 3833 3996 646
Size: 961 2045 181 163 2352
Fragments arranged by size:
2352 2045 961 181 163
BtsI GCAGTG_nn'
Cuts at: 666 1905 2945 666
Size: 1239 1040 3423
Fragments arranged by size:
3423 1239 1040
DraI TTT'AAA
Cuts at: 2701 2701
Size: 5702
DraIII CAC_nnn'GTG
Cuts at: 627 627
Size: 5702
DrdI GACnn_nn'nnGTC
Cuts at: 405 3408 4752 405
Size: 3003 1344 1355
Fragments arranged by size:
3003 1355 1344
EaeI y'GGCC_r
Cuts at: 438 576 937 986 1355 1439 1613 3287 3461
Size: 138 361 49 369 84 174 1674 174
Cuts at: 3461 3852 3879 5224 438
Size: 391 27 1345 916
Fragments arranged by size:
1674 1345 916 391 369 361 174 174
138 84 49 27
EagI C'GGCC_G
Cuts at: 937 3287 937
Size: 2350 3352
EarI CTCTTCn'nnn_
Cuts at: 1409 2531 3725 3935 1409
Size: 1122 1194 210 3176
Fragments arranged by size:
3176 1194 1122 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3910 4652 4798 5512 3910
Size: 742 146 714 4100
Fragments arranged by size:
4100 742 714 146
EcoICRI GAG'CTC
Cuts at: 543 1715 2331 2391 2714 543
Size: 1172 616 60 323 3531
Fragments arranged by size:
3531 1172 616 323 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 675 867 1059 1384 1406 1711 2221 2242
Size: 184 192 192 325 22 305 510 21
Cuts at: 2242 2380 3527 3959 4312 4974 491
Size: 138 1147 432 353 662 1219
Fragments arranged by size:
1219 1147 662 510 432 353 325 305
192 192 184 138 22 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2248 2515 2248
Size: 267 5435
EcoO109I rG'GnC_Cy
Cuts at: 468 803 1209 1876 2841 468
Size: 335 406 667 965 3329
Fragments arranged by size:
3329 965 667 406 335
EcoRI G'AATT_C
Cuts at: 2706 2706
Size: 5702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5702
FspI TGC'GCA
Cuts at: 600 3483 600
Size: 2883 2819
HaeII r_GCGC'y
Cuts at: 636 1539 1633 1664 1907 2187 2370 3136 3384
Size: 903 94 31 243 280 183 766 248
Cuts at: 3384 4614 5385 636
Size: 1230 771 953
Fragments arranged by size:
1230 953 903 771 766 280 248 243
183 94 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 324 349 356 381 1633 1665 3544 3574 3576
Size: 25 7 25 1252 32 1879 30 2
Cuts at: 3576 3606 324
Size: 30 2420
Fragments arranged by size:
2420 1879 1252 32 30 30 25 25
7 2
HincII GTy'rAC
Cuts at: 2741 2741
Size: 5702
KasI G'GCGC_C
Cuts at: 1903 3132 3380 1903
Size: 1229 248 4225
Fragments arranged by size:
4225 1229 248
KpnI G_GTAC'C
Cuts at: 1748 2722 1748
Size: 974 4728
MluI A'CGCG_T
Cuts at: 5135 5135
Size: 5702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1911 2534 4461 4645 1911
Size: 623 1927 184 2968
Fragments arranged by size:
2968 1927 623 184
MscI TGG'CCA
Cuts at: 440 988 1441 3463 440
Size: 548 453 2022 2679
Fragments arranged by size:
2679 2022 548 453
MslI CAynn'nnrTG
Cuts at: 508 742 1228 2272 2635 3691 3818 508
Size: 234 486 1044 363 1056 127 2392
Fragments arranged by size:
2392 1056 1044 486 363 234 127
MspA1I CmG'CkG
Cuts at: 120 167 1426 1702 1977 2813 3127 3487 4271
Size: 47 1259 276 275 836 314 360 784
Cuts at: 4271 4516 120
Size: 245 1306
Fragments arranged by size:
1306 1259 836 784 360 314 276 275
245 47
NaeI GCC'GGC
Cuts at: 2188 3883 2188
Size: 1695 4007
NarI GG'CG_CC
Cuts at: 1904 3133 3381 1904
Size: 1229 248 4225
Fragments arranged by size:
4225 1229 248
NcoI C'CATG_G
Cuts at: 107 1216 2160 107
Size: 1109 944 3649
Fragments arranged by size:
3649 1109 944
NgoMIV G'CCGG_C
Cuts at: 2186 3881 2186
Size: 1695 4007
NspI r_CATG'y
Cuts at: 619 2271 2755 3255 4858 619
Size: 1652 484 500 1603 1463
Fragments arranged by size:
1652 1603 1463 500 484
PasI CC'CwG_GG
Cuts at: 706 1455 706
Size: 749 4953
PciI A'CATG_T
Cuts at: 4854 4854
Size: 5702
PfoI T'CCnGG_A
Cuts at: 200 344 864 200
Size: 144 520 5038
Fragments arranged by size:
5038 520 144
PmeI GTTT'AAAC
Cuts at: 2701 2701
Size: 5702
PmlI CAC'GTG
Cuts at: 596 596
Size: 5702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 4148
Size: 32 5670
PpuMI rG'GwC_Cy
Cuts at: 1209 1876 1209
Size: 667 5035
PspOMI G'GGCC_C
Cuts at: 771 802 2441 771
Size: 31 1639 4032
Fragments arranged by size:
4032 1639 31
PstI C_TGCA'G
Cuts at: 433 897 1085 1677 1900 1982 2034 2749 3434
Size: 464 188 592 223 82 52 715 685
Cuts at: 3434 433
Size: 2701
Fragments arranged by size:
2701 715 685 592 464 223 188 82
52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5702
PvuII CAG'CTG
Cuts at: 1426 1702 1977 3127 3487 1426
Size: 276 275 1150 360 3641
Fragments arranged by size:
3641 1150 360 276 275
RsrII CG'GwC_CG
Cuts at: 3897 3897
Size: 5702
SacI G_AGCT'C
Cuts at: 545 1717 2333 2393 2716 545
Size: 1172 616 60 323 3531
Fragments arranged by size:
3531 1172 616 323 60
SacII CC_GC'GG
Cuts at: 121 168 121
Size: 47 5655
SalI G'TCGA_C
Cuts at: 2739 2739
Size: 5702
SapI GCTCTTCn'nnn_
Cuts at: 3725 3935 3725
Size: 210 5492
SbfI CC_TGCA'GG
Cuts at: 897 2749 897
Size: 1852 3850
ScaI AGT'ACT
Cuts at: 5219 5219
Size: 5702
SexAI A'CCwGG_T
Cuts at: 1094 1094
Size: 5702
SfcI C'TryA_G
Cuts at: 33 429 494 893 976 1081 1673 1896 1978
Size: 396 65 399 83 105 592 223 82
Cuts at: 1978 2030 2745 3430 4398 4589 33
Size: 52 715 685 968 191 1146
Fragments arranged by size:
1146 968 715 685 592 399 396 223
191 105 83 82 65 52
SfoI GGC'GCC
Cuts at: 1905 3134 3382 1905
Size: 1229 248 4225
Fragments arranged by size:
4225 1229 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5702
SmaI CCC'GGG
Cuts at: 776 961 1283 2446 2724 776
Size: 185 322 1163 278 3754
Fragments arranged by size:
3754 1163 322 278 185
SmlI C'TyrA_G
Cuts at: 1832 2857 3772 4486 4748 1832
Size: 1025 915 714 262 2786
Fragments arranged by size:
2786 1025 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1803 67
Size: 1736 3966
SphI G_CATG'C
Cuts at: 2755 3255 2755
Size: 500 5202
StuI AGG'CCT
Cuts at: 798 1030 798
Size: 232 5470
StyI C'CwwG_G
Cuts at: 107 272 854 917 1216 2060 2160 2836 3190
Size: 165 582 63 299 844 100 676 354
Cuts at: 3190 107
Size: 2619
Fragments arranged by size:
2619 844 676 582 354 299 165 100
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 474 516 1446 1491 1569 3285 3418 3877 4086
Size: 42 930 45 78 1716 133 459 209
Cuts at: 4086 474
Size: 2090
Fragments arranged by size:
2090 1716 930 459 209 133 78 45
42
TatI w'GTAC_w
Cuts at: 2138 5217 2138
Size: 3079 2623
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1453 1485 1559 1591 5219 5251 1453
Size: 32 74 32 3628 32 1904
Fragments arranged by size:
3628 1904 74 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 280 361 783 3499 280
Size: 81 422 2716 2483
Fragments arranged by size:
2716 2483 422 81
XbaI T'CTAG_A
Cuts at: 2733 2733
Size: 5702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1452 1782 2420 3197 1452
Size: 330 638 777 3957
Fragments arranged by size:
3957 777 638 330
XmaI C'CCGG_G
Cuts at: 774 959 1281 2444 2722 774
Size: 185 322 1163 278 3754
Fragments arranged by size:
3754 1163 322 278 185
XmnI GAAnn'nnTTC
Cuts at: 2578 2578
Size: 5702
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PshAI PsiI PspXI PsrI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI