Restriction map for
pF1KA1673
(Circular) (Six-base) MAPSORT of: pF1KA1673.seq Check: 9971 from: 1 to: 5249
pF1KA1673 5249 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2287 2287
Size: 5249
Acc65I G'GTAC_C
Cuts at: 2265 2265
Size: 5249
AclI AA'CG_TT
Cuts at: 2243 5089 2243
Size: 2846 2403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1416 3074 3506 4253 4521 1416
Size: 1658 432 747 268 2144
Fragments arranged by size:
2144 1658 747 432 268
AflIII A'CryG_T
Cuts at: 2601 3705 4682 2601
Size: 1104 977 3168
Fragments arranged by size:
3168 1104 977
AgeI A'CCGG_T
Cuts at: 3637 3637
Size: 5249
AlwNI CAG_nnn'CTG
Cuts at: 617 2554 4121 617
Size: 1937 1567 1745
Fragments arranged by size:
1937 1745 1567
ApaI G_GGCC'C
Cuts at: 2076 2076
Size: 5249
ApaLI G'TGCA_C
Cuts at: 4019 4019
Size: 5249
ApoI r'AATT_y
Cuts at: 732 1116 2089 2179 2253 4602 4858 732
Size: 384 973 90 74 2349 256 1123
Fragments arranged by size:
2349 1123 973 384 256 90 74
AseI AT'TA_AT
Cuts at: 20 20
Size: 5249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5249
AvaI C'yCGr_G
Cuts at: 1008 2269 1008
Size: 1261 3988
BamHI G'GATC_C
Cuts at: 2274 2274
Size: 5249
BanI G'GyrC_C
Cuts at: 2265 2679 2927 2962 2265
Size: 414 248 35 4552
Fragments arranged by size:
4552 414 248 35
BanII G_rGCy'C
Cuts at: 1009 2076 2263 3293 1009
Size: 1067 187 1030 2965
Fragments arranged by size:
2965 1067 1030 187
BbeI G_GCGC'C
Cuts at: 2683 2931 2683
Size: 248 5001
BbsI GAAGACnn'nnnn_
Cuts at: 1337 1337
Size: 5249
BciVI GTATCCnnnnn_n'
Cuts at: 3147 3908 5249 3147
Size: 761 1341 3147
Fragments arranged by size:
3147 1341 761
BclI T'GATC_A
Cuts at: 2046 2769 2046
Size: 723 4526
BfrBI ATG'CAT
Cuts at: 1609 1657 1661 1609
Size: 48 4 5197
Fragments arranged by size:
5197 48 4
BglII A'GATC_T
Cuts at: 326 2764 4801 326
Size: 2438 2037 774
Fragments arranged by size:
2438 2037 774
BlpI GC'TnA_GC
Cuts at: 2361 2361
Size: 5249
Bme1580I G_kGCm'C
Cuts at: 1980 2076 2874 2967 4023 1980
Size: 96 798 93 1056 3206
Fragments arranged by size:
3206 1056 798 96 93
BmrI ACTGGGnnnn_n'
Cuts at: 158 563 2461 2542 2877 5021 158
Size: 405 1898 81 335 2144 386
Fragments arranged by size:
2144 1898 405 386 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3616 3616
Size: 5249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 503 1023 1126 1285 1481 284
Size: 219 520 103 159 196 4052
Fragments arranged by size:
4052 520 219 196 159 103
Bpu10I CC'TnA_GC
Cuts at: 1904 1904
Size: 5249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 3304 3796 4094 2425
Size: 879 492 298 3580
Fragments arranged by size:
3580 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 866 4953 866
Size: 4087 1162
BsaAI yAC'GTr
Cuts at: 2602 2602
Size: 5249
BsaBI GATnn'nnATC
Cuts at: 6 2763 4800 6
Size: 2757 2037 455
Fragments arranged by size:
2757 2037 455
BsaHI Gr'CG_yC
Cuts at: 2680 2928 3630 5126 2680
Size: 248 702 1496 2803
Fragments arranged by size:
2803 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1230 2438 2959 3637 3911 4058 4469 1230
Size: 1208 521 678 274 147 411 2010
Fragments arranged by size:
2010 1208 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 119 149 119
Size: 30 5219
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 633 767 984 633
Size: 134 217 4898
Fragments arranged by size:
4898 217 134
BseYI C'CCAG_C
Cuts at: 223 274 1031 1159 1885 1927 2147 2674 3432
Size: 51 757 128 726 42 220 527 758
Cuts at: 3432 4009 4505 223
Size: 577 496 967
Fragments arranged by size:
967 758 757 726 577 527 496 220
128 51 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1660 1660
Size: 5249
BsiEI CG_ry'CG
Cuts at: 104 2791 2837 4045 104
Size: 2687 46 1208 1308
Fragments arranged by size:
2687 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 720 2263 3041 3231 4023 720
Size: 1543 778 190 792 1946
Fragments arranged by size:
1946 1543 792 778 190
BsmI GAATG_Cn'
Cuts at: 1609 1609
Size: 5249
BsmBI CGTCTCn'nnnn_
Cuts at: 602 602
Size: 5249
Bsp1286I G_dGCh'C
Cuts at: 720 1009 1980 2076 2263 2874 2967 3041 3231
Size: 289 971 96 187 611 93 74 190
Cuts at: 3231 3293 4023 720
Size: 62 730 1946
Fragments arranged by size:
1946 971 730 611 289 190 187 96
93 74 62
BspEI T'CCGG_A
Cuts at: 1230 4469 1230
Size: 3239 2010
BspHI T'CATG_A
Cuts at: 4425 4425
Size: 5249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2299 2513 2815 3196 2299
Size: 214 302 381 4352
Fragments arranged by size:
4352 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3541 3595 5079 5244 50
Size: 3491 54 1484 165 55
Fragments arranged by size:
3491 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 495 3161 495
Size: 2666 2583
BsrFI r'CCGG_y
Cuts at: 3247 3428 3637 3247
Size: 181 209 4859
Fragments arranged by size:
4859 209 181
BssSI C'ACGA_G
Cuts at: 2165 3520 3878 2165
Size: 1355 358 3536
Fragments arranged by size:
3536 1355 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2626 2626
Size: 5249
BstBI TT'CG_AA
Cuts at: 3610 3610
Size: 5249
BstEII G'GTnAC_C
Cuts at: 2209 2209
Size: 5249
BstYI r'GATC_y
Cuts at: 1 326 2274 2741 2764 3099 3345 4357 4443
Size: 325 1948 467 23 335 246 1012 86
Cuts at: 4443 4455 4801 1
Size: 12 346 449
Fragments arranged by size:
1948 1012 467 449 346 335 325 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 958 1386 107
Size: 851 428 3970
Fragments arranged by size:
3970 851 428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1861 3199 3380 3543 1861
Size: 1338 181 163 3567
Fragments arranged by size:
3567 1338 181 163
BtsI GCAGTG_nn'
Cuts at: 276 1425 2492 276
Size: 1149 1067 3033
Fragments arranged by size:
3033 1149 1067
DraI TTT'AAA
Cuts at: 2248 2248
Size: 5249
DrdI GACnn_nn'nnGTC
Cuts at: 2955 3813 2955
Size: 858 4391
EaeI y'GGCC_r
Cuts at: 1383 2834 3008 3399 3426 4771 1383
Size: 1451 174 391 27 1345 1861
Fragments arranged by size:
1861 1451 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2834 2834
Size: 5249
EarI CTCTTCn'nnn_
Cuts at: 1002 1406 3272 3482 1002
Size: 404 1866 210 2769
Fragments arranged by size:
2769 1866 404 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1502 3457 3767 3913 5059 1502
Size: 1955 310 146 1146 1692
Fragments arranged by size:
1955 1692 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2261 2261
Size: 5249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 503 1023 1126 1285 1416 1481 3074 3506
Size: 219 520 103 159 131 65 1593 432
Cuts at: 3506 4253 4521 284
Size: 747 268 1012
Fragments arranged by size:
1593 1012 747 520 432 268 219 159
131 103 65
EcoNI CCTnn'n_nnAGG
Cuts at: 1598 2197 1598
Size: 599 4650
EcoO109I rG'GnC_Cy
Cuts at: 690 2072 2388 690
Size: 1382 316 3551
Fragments arranged by size:
3551 1382 316
EcoRI G'AATT_C
Cuts at: 2179 2253 2179
Size: 74 5175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 5249
FspI TGC'GCA
Cuts at: 3030 3030
Size: 5249
HaeII r_GCGC'y
Cuts at: 637 2683 2931 3953 4932 637
Size: 2046 248 1022 979 954
Fragments arranged by size:
2046 1022 979 954 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 774 806 1243 1275 1567 1599 1618
Size: 32 241 32 437 32 292 32 19
Cuts at: 1618 1650 1842 1874 3091 3121 3123 3153 501
Size: 32 192 32 1217 30 2 30 2597
Fragments arranged by size:
2597 1217 437 292 241 192 32 32
32 32 32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 527 2288 527
Size: 1761 3488
KasI G'GCGC_C
Cuts at: 2679 2927 2679
Size: 248 5001
KpnI G_GTAC'C
Cuts at: 2269 2269
Size: 5249
MluI A'CGCG_T
Cuts at: 4682 4682
Size: 5249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3920 4104 3920
Size: 184 5065
MscI TGG'CCA
Cuts at: 3010 3010
Size: 5249
MslI CAynn'nnrTG
Cuts at: 212 899 1442 2165 3238 3365 212
Size: 687 543 723 1073 127 2096
Fragments arranged by size:
2096 1073 723 687 543 127
MspA1I CmG'CkG
Cuts at: 256 918 1992 2052 2237 2360 2674 3034 4047
Size: 662 1074 60 185 123 314 360 1013
Cuts at: 4047 4292 256
Size: 245 1213
Fragments arranged by size:
1213 1074 1013 662 360 314 245 185
123 60
NaeI GCC'GGC
Cuts at: 3430 3430
Size: 5249
NarI GG'CG_CC
Cuts at: 2680 2928 2680
Size: 248 5001
NcoI C'CATG_G
Cuts at: 107 107
Size: 5249
NdeI CA'TA_TG
Cuts at: 1323 2034 1323
Size: 711 4538
NgoMIV G'CCGG_C
Cuts at: 3428 3428
Size: 5249
NsiI A_TGCA'T
Cuts at: 1611 1659 1663 1611
Size: 48 4 5197
Fragments arranged by size:
5197 48 4
NspI r_CATG'y
Cuts at: 1257 1661 2302 2802 3709 1257
Size: 404 641 500 907 2797
Fragments arranged by size:
2797 907 641 500 404
PciI A'CATG_T
Cuts at: 3705 3705
Size: 5249
PfoI T'CCnGG_A
Cuts at: 1000 1183 1000
Size: 183 5066
PmeI GTTT'AAAC
Cuts at: 2248 2248
Size: 5249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1084 1116 4388 4420 1084
Size: 32 3272 32 1913
Fragments arranged by size:
3272 1913 32 32
PpuMI rG'GwC_Cy
Cuts at: 690 690
Size: 5249
PsiI TTA'TAA
Cuts at: 241 241
Size: 5249
PspOMI G'GGCC_C
Cuts at: 2072 2072
Size: 5249
PstI C_TGCA'G
Cuts at: 194 1997 2129 2296 2981 194
Size: 1803 132 167 685 2462
Fragments arranged by size:
2462 1803 685 167 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5249
PvuII CAG'CTG
Cuts at: 256 918 1992 2052 2674 3034 256
Size: 662 1074 60 622 360 2471
Fragments arranged by size:
2471 1074 662 622 360 60
RsrII CG'GwC_CG
Cuts at: 3444 3444
Size: 5249
SacI G_AGCT'C
Cuts at: 2263 2263
Size: 5249
SalI G'TCGA_C
Cuts at: 2286 2286
Size: 5249
SanDI GG'GwC_CC
Cuts at: 690 690
Size: 5249
SapI GCTCTTCn'nnn_
Cuts at: 3272 3482 3272
Size: 210 5039
SbfI CC_TGCA'GG
Cuts at: 2296 2296
Size: 5249
ScaI AGT'ACT
Cuts at: 4766 4766
Size: 5249
SfcI C'TryA_G
Cuts at: 33 190 1993 2125 2292 2977 3970 4161 33
Size: 157 1803 132 167 685 993 191 1121
Fragments arranged by size:
1803 1121 993 685 191 167 157 132
SfoI GGC'GCC
Cuts at: 2681 2929 2681
Size: 248 5001
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5249
SmaI CCC'GGG
Cuts at: 1010 2271 1010
Size: 1261 3988
SmlI C'TyrA_G
Cuts at: 2404 3319 3811 4073 2404
Size: 915 492 262 3580
Fragments arranged by size:
3580 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5249
SphI G_CATG'C
Cuts at: 1661 2302 2802 1661
Size: 641 500 4108
Fragments arranged by size:
4108 641 500
SrfI GCCC'GGGC
Cuts at: 1010 1010
Size: 5249
SspI AAT'ATT
Cuts at: 2143 2143
Size: 5249
StyI C'CwwG_G
Cuts at: 107 1044 1735 2383 2737 107
Size: 937 691 648 354 2619
Fragments arranged by size:
2619 937 691 648 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 821 2832 2965 3424 3633 821
Size: 2011 133 459 209 2437
Fragments arranged by size:
2437 2011 459 209 133
TatI w'GTAC_w
Cuts at: 4764 4764
Size: 5249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 119 151 1731 1763 4766 4798 119
Size: 32 1580 32 3003 32 570
Fragments arranged by size:
3003 1580 570 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3046 3046
Size: 5249
XbaI T'CTAG_A
Cuts at: 2280 2280
Size: 5249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 2744 2167
Size: 577 4672
XmaI C'CCGG_G
Cuts at: 1008 2269 1008
Size: 1261 3988
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI StuI SwaI
XhoI XmnI ZraI