Restriction map for
pF1KA1675
(Circular) (Six-base) MAPSORT of: pF1KA1675.seq Check: 1153 from: 1 to: 6941
pF1KA1675 6941 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 1928 1928
Size: 6941
AccI GT'mk_AC
Cuts at: 323 749 3979 323
Size: 426 3230 3285
Fragments arranged by size:
3285 3230 426
Acc65I G'GTAC_C
Cuts at: 3957 3957
Size: 6941
AclI AA'CG_TT
Cuts at: 6781 6781
Size: 6941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1147 1900 2773 3054 4766 5198 5945 6213
Size: 794 753 873 281 1712 432 747 268
Cuts at: 6213 353
Size: 1081
Fragments arranged by size:
1712 1081 873 794 753 747 432 281
268
AflIII A'CryG_T
Cuts at: 860 862 1898 2960 4293 5397 6374 860
Size: 2 1036 1062 1333 1104 977 1427
Fragments arranged by size:
1427 1333 1104 1062 1036 977 2
AgeI A'CCGG_T
Cuts at: 5329 5329
Size: 6941
AhdI GACnn_n'nnGTC
Cuts at: 3534 3534
Size: 6941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2887 2887
Size: 6941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 778 810 778
Size: 32 6909
AlwNI CAG_nnn'CTG
Cuts at: 388 2154 2295 2514 2972 4246 5813 388
Size: 1766 141 219 458 1274 1567 1516
Fragments arranged by size:
1766 1567 1516 1274 458 219 141
ApaI G_GGCC'C
Cuts at: 442 1203 1832 2082 2482 2541 442
Size: 761 629 250 400 59 4842
Fragments arranged by size:
4842 761 629 400 250 59
ApaLI G'TGCA_C
Cuts at: 730 5711 730
Size: 4981 1960
ApoI r'AATT_y
Cuts at: 3945 6294 6550 3945
Size: 2349 256 4336
Fragments arranged by size:
4336 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6941
AvaI C'yCGr_G
Cuts at: 458 916 1196 1482 1590 1650 1831 2073 2381
Size: 458 280 286 108 60 181 242 308
Cuts at: 2381 2859 3229 3547 3961 458
Size: 478 370 318 414 3438
Fragments arranged by size:
3438 478 458 414 370 318 308 286
280 242 181 108 60
BamHI G'GATC_C
Cuts at: 3966 3966
Size: 6941
BanI G'GyrC_C
Cuts at: 453 845 1614 2067 2100 2167 2608 3182 3957
Size: 392 769 453 33 67 441 574 775
Cuts at: 3957 4371 4619 4654 453
Size: 414 248 35 2740
Fragments arranged by size:
2740 775 769 574 453 441 414 392
248 67 35 33
BanII G_rGCy'C
Cuts at: 179 304 365 442 791 911 1203 1720 1832
Size: 125 61 77 349 120 292 517 112
Cuts at: 1832 1975 2082 2260 2340 2482 2541 2573 2751
Size: 143 107 178 80 142 59 32 178
Cuts at: 2751 3554 3955 4985 179
Size: 803 401 1030 2135
Fragments arranged by size:
2135 1030 803 517 401 349 292 178
178 143 142 125 120 112 107 80
77 61 59 32
BbeI G_GCGC'C
Cuts at: 849 1618 2071 2171 2612 4375 4623 849
Size: 769 453 100 441 1763 248 3167
Fragments arranged by size:
3167 1763 769 453 441 248 100
BbsI GAAGACnn'nnnn_
Cuts at: 341 560 1231 1888 2100 3320 3839 341
Size: 219 671 657 212 1220 519 3443
Fragments arranged by size:
3443 1220 671 657 519 219 212
BbvCI CC'TCA_GC
Cuts at: 902 3635 3713 902
Size: 2733 78 4130
Fragments arranged by size:
4130 2733 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 211 245 1098 1132 2945 2979 90
Size: 34 87 34 853 34 1813 34 4052
Fragments arranged by size:
4052 1813 853 87 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4839 5600 6941 4839
Size: 761 1341 4839
Fragments arranged by size:
4839 1341 761
BclI T'GATC_A
Cuts at: 4461 4461
Size: 6941
BglI GCCn_nnn'nGGC
Cuts at: 2555 2851 3601 2555
Size: 296 750 5895
Fragments arranged by size:
5895 750 296
BglII A'GATC_T
Cuts at: 545 2981 4456 6493 545
Size: 2436 1475 2037 993
Fragments arranged by size:
2436 2037 1475 993
BlpI GC'TnA_GC
Cuts at: 4053 4053
Size: 6941
Bme1580I G_kGCm'C
Cuts at: 442 458 734 763 1203 1813 1832 2082 2422
Size: 16 276 29 440 610 19 250 340
Cuts at: 2422 2482 2541 2638 3423 4566 4659 5715 442
Size: 60 59 97 785 1143 93 1056 1668
Fragments arranged by size:
1668 1143 1056 785 610 440 340 276
250 97 93 60 59 29 19 16
BmgBI CAC'GTC
Cuts at: 510 1238 1992 2451 2586 3099 3459 3899 510
Size: 728 754 459 135 513 360 440 3552
Fragments arranged by size:
3552 754 728 513 459 440 360 135
BmrI ACTGGGnnnn_n'
Cuts at: 1745 2402 3038 3417 4153 4234 4569 6713 1745
Size: 657 636 379 736 81 335 2144 1973
Fragments arranged by size:
2144 1973 736 657 636 379 335 81
BmtI G_CTAG'C
Cuts at: 139 139
Size: 6941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5308 5308
Size: 6941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1563 1876 2851 3073 3505 3911 3929 1015
Size: 548 313 975 222 432 406 18 4027
Fragments arranged by size:
4027 975 548 432 406 313 222 18
Bpu10I CC'TnA_GC
Cuts at: 902 2521 3635 3713 902
Size: 1619 1114 78 4130
Fragments arranged by size:
4130 1619 1114 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 895 1234 1696 2377 3172 4117 4996 5488
Size: 297 339 462 681 795 945 879 492
Cuts at: 5488 5786 598
Size: 298 1753
Fragments arranged by size:
1753 945 879 795 681 492 462 339
298 297
BsaI GGTCTCn'nnnn_
Cuts at: 1788 2806 6645 1788
Size: 1018 3839 2084
Fragments arranged by size:
3839 2084 1018
BsaAI yAC'GTr
Cuts at: 735 863 1188 2963 4294 735
Size: 128 325 1775 1331 3382
Fragments arranged by size:
3382 1775 1331 325 128
BsaBI GATnn'nnATC
Cuts at: 6 587 4455 6492 6
Size: 581 3868 2037 455
Fragments arranged by size:
3868 2037 581 455
BsaHI Gr'CG_yC
Cuts at: 746 846 1615 1739 1925 2068 2168 2387 2609
Size: 100 769 124 186 143 100 219 222
Cuts at: 2609 4372 4620 5322 6818 746
Size: 1763 248 702 1496 869
Fragments arranged by size:
1763 1496 869 769 702 248 222 219
186 143 124 100 100
BsaWI w'CCGG_w
Cuts at: 2903 3101 4130 4651 5329 5603 5750 6161 2903
Size: 198 1029 521 678 274 147 411 3683
Fragments arranged by size:
3683 1029 678 521 411 274 198 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 778 808 1015 1045 1561 1591 1602 1632 3025
Size: 30 207 30 516 30 11 30 1393
Cuts at: 3025 3055 3838 3868 778
Size: 30 783 30 3851
Fragments arranged by size:
3851 1393 783 516 207 30 30 30
30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 733 799 919 1036 1404 1849 2518 3478 3481
Size: 66 120 117 368 445 669 960 3
Cuts at: 3481 3502 3589 3598 3830 733
Size: 21 87 9 232 3844
Fragments arranged by size:
3844 960 669 445 368 232 120 117
87 66 21 9 3
BseYI C'CCAG_C
Cuts at: 348 435 1153 1501 1534 1767 2117 2253 2604
Size: 87 718 348 33 233 350 136 351
Cuts at: 2604 2651 4366 5124 5701 6197 348
Size: 47 1715 758 577 496 1092
Fragments arranged by size:
1715 1092 758 718 577 496 351 350
348 233 136 87 47 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2985 3804 2985
Size: 819 6122
BsiEI CG_ry'CG
Cuts at: 104 1684 2773 3063 4483 4529 5737 104
Size: 1580 1089 290 1420 46 1208 1308
Fragments arranged by size:
1580 1420 1308 1208 1089 290 46
BsiHKAI G_wGCw'C
Cuts at: 365 637 734 911 3203 3955 4733 4923 5715
Size: 272 97 177 2292 752 778 190 792
Cuts at: 5715 365
Size: 1591
Fragments arranged by size:
2292 1591 792 778 752 272 190 177
97
BsiWI C'GTAC_G
Cuts at: 308 308
Size: 6941
Bsp1286I G_dGCh'C
Cuts at: 179 304 365 442 458 637 734 763 791
Size: 125 61 77 16 179 97 29 28
Cuts at: 791 911 1203 1720 1813 1832 1975 2082 2260
Size: 120 292 517 93 19 143 107 178
Cuts at: 2260 2340 2422 2482 2541 2573 2638 2751 3203
Size: 80 82 60 59 32 65 113 452
Cuts at: 3203 3423 3554 3955 4566 4659 4733 4923 4985
Size: 220 131 401 611 93 74 190 62
Cuts at: 4985 5715 179
Size: 730 1405
Fragments arranged by size:
1405 730 611 517 452 401 292 220
190 179 178 143 131 125 120 113
107 97 93 93 82 80 77 74
65 62 61 60 59 32 29 28
19 16
BspEI T'CCGG_A
Cuts at: 3101 6161 3101
Size: 3060 3881
BspHI T'CATG_A
Cuts at: 6117 6117
Size: 6941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 848 2774 2957 3991 4205 4507 4888 197
Size: 651 1926 183 1034 214 302 381 2250
Fragments arranged by size:
2250 1926 1034 651 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 969 1178 2664 3065 5233 5287 6771 6936
Size: 919 209 1486 401 2168 54 1484 165
Cuts at: 6936 50
Size: 55
Fragments arranged by size:
2168 1486 1484 919 401 209 165 55
54
BsrDI GCAATG_nn'
Cuts at: 2201 3818 4853 2201
Size: 1617 1035 4289
Fragments arranged by size:
4289 1617 1035
BsrFI r'CCGG_y
Cuts at: 1337 1430 2611 3179 4939 5120 5329 1337
Size: 93 1181 568 1760 181 209 2949
Fragments arranged by size:
2949 1760 1181 568 209 181 93
BssSI C'ACGA_G
Cuts at: 949 3196 3202 5212 5570 949
Size: 2247 6 2010 358 2320
Fragments arranged by size:
2320 2247 2010 358 6
BstAPI GCAn_nnn'nTGC
Cuts at: 962 2203 3152 4318 962
Size: 1241 949 1166 3585
Fragments arranged by size:
3585 1241 1166 949
BstBI TT'CG_AA
Cuts at: 1118 3082 5302 1118
Size: 1964 2220 2757
Fragments arranged by size:
2757 2220 1964
BstXI CCAn_nnnn'nTGG
Cuts at: 249 1758 2590 249
Size: 1509 832 4600
Fragments arranged by size:
4600 1509 832
BstYI r'GATC_y
Cuts at: 1 545 2981 3966 4433 4456 4791 5037 6049
Size: 544 2436 985 467 23 335 246 1012
Cuts at: 6049 6135 6147 6493 1
Size: 86 12 346 449
Fragments arranged by size:
2436 1012 985 544 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2938 3016 2938
Size: 78 6863
BtgI C'CryG_G
Cuts at: 248 1610 1937 1961 2741 3605 248
Size: 1362 327 24 780 864 3584
Fragments arranged by size:
3584 1362 864 780 327 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 656 2178 4891 5072 5235 656
Size: 1522 2713 181 163 2362
Fragments arranged by size:
2713 2362 1522 181 163
BtsI GCAGTG_nn'
Cuts at: 570 1917 3423 4184 570
Size: 1347 1506 761 3327
Fragments arranged by size:
3327 1506 1347 761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1371 1406 1371
Size: 35 6906
DraI TTT'AAA
Cuts at: 3770 3940 3770
Size: 170 6771
DraIII CAC_nnn'GTG
Cuts at: 662 662
Size: 6941
DrdI GACnn_nn'nnGTC
Cuts at: 3261 4647 5505 3261
Size: 1386 858 4697
Fragments arranged by size:
4697 1386 858
EaeI y'GGCC_r
Cuts at: 194 939 1392 1681 2093 2770 3509 3602 4526
Size: 745 453 289 412 677 739 93 924
Cuts at: 4526 4700 5091 5118 6463 194
Size: 174 391 27 1345 672
Fragments arranged by size:
1345 924 745 739 677 672 453 412
391 289 174 93 27
EagI C'GGCC_G
Cuts at: 1681 2770 4526 1681
Size: 1089 1756 4096
Fragments arranged by size:
4096 1756 1089
EarI CTCTTCn'nnn_
Cuts at: 826 2791 3084 4964 5174 826
Size: 1965 293 1880 210 2593
Fragments arranged by size:
2593 1965 1880 293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1072 1776 2022 2869 3409 5149 5459 5605 6751
Size: 704 246 847 540 1740 310 146 1146
Cuts at: 6751 1072
Size: 1262
Fragments arranged by size:
1740 1262 1146 847 704 540 310 246
146
EcoICRI GAG'CTC
Cuts at: 363 909 3953 363
Size: 546 3044 3351
Fragments arranged by size:
3351 3044 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1015 1147 1563 1876 1900 2773 2851 3054
Size: 662 132 416 313 24 873 78 203
Cuts at: 3054 3073 3505 3911 3929 4766 5198 5945 6213
Size: 19 432 406 18 837 432 747 268
Cuts at: 6213 353
Size: 1081
Fragments arranged by size:
1081 873 837 747 662 432 432 416
406 313 268 203 132 78 24 19
18
EcoNI CCTnn'n_nnAGG
Cuts at: 781 901 2941 3487 781
Size: 120 2040 546 4235
Fragments arranged by size:
4235 2040 546 120
EcoO109I rG'GnC_Cy
Cuts at: 186 315 439 894 921 1828 2078 2079 2478
Size: 129 124 455 27 907 250 1 399
Cuts at: 2478 2479 3234 4080 186
Size: 1 755 846 3047
Fragments arranged by size:
3047 907 846 755 455 399 250 129
124 27 1 1
EcoRI G'AATT_C
Cuts at: 3945 3945
Size: 6941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3757 4960 3757
Size: 1203 5738
FspI TGC'GCA
Cuts at: 4722 4722
Size: 6941
HaeII r_GCGC'y
Cuts at: 849 1196 1437 1618 2071 2171 2612 4375 4623
Size: 347 241 181 453 100 441 1763 248
Cuts at: 4623 5645 6624 849
Size: 1022 979 1166
Fragments arranged by size:
1763 1166 1022 979 453 441 347 248
241 181 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2359 2391 3520 3552 4783 4813 4815 4845 2359
Size: 32 1129 32 1231 30 2 30 4455
Fragments arranged by size:
4455 1231 1129 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1929 3980 1929
Size: 2051 4890
KasI G'GCGC_C
Cuts at: 845 1614 2067 2167 2608 4371 4619 845
Size: 769 453 100 441 1763 248 3167
Fragments arranged by size:
3167 1763 769 453 441 248 100
KpnI G_GTAC'C
Cuts at: 3961 3961
Size: 6941
MluI A'CGCG_T
Cuts at: 6374 6374
Size: 6941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1097 1352 1883 2951 3263 3881 5612 5796 1097
Size: 255 531 1068 312 618 1731 184 2242
Fragments arranged by size:
2242 1731 1068 618 531 312 255 184
MscI TGG'CCA
Cuts at: 196 1394 4702 196
Size: 1198 3308 2435
Fragments arranged by size:
3308 2435 1198
MslI CAynn'nnrTG
Cuts at: 247 625 2236 2588 4930 5057 247
Size: 378 1611 352 2342 127 2131
Fragments arranged by size:
2342 2131 1611 378 352 127
MspA1I CmG'CkG
Cuts at: 608 800 938 1106 1167 1707 1822 2151 2514
Size: 192 138 168 61 540 115 329 363
Cuts at: 2514 2781 3093 3152 3712 4052 4366 4726 5739
Size: 267 312 59 560 340 314 360 1013
Cuts at: 5739 5984 608
Size: 245 1565
Fragments arranged by size:
1565 1013 560 540 363 360 340 329
314 312 267 245 192 168 138 115
61 59
NaeI GCC'GGC
Cuts at: 1339 2613 5122 1339
Size: 1274 2509 3158
Fragments arranged by size:
3158 2509 1274
NarI GG'CG_CC
Cuts at: 846 1615 2068 2168 2609 4372 4620 846
Size: 769 453 100 441 1763 248 3167
Fragments arranged by size:
3167 1763 769 453 441 248 100
NdeI CA'TA_TG
Cuts at: 1291 3807 1291
Size: 2516 4425
NgoMIV G'CCGG_C
Cuts at: 1337 2611 5120 1337
Size: 1274 2509 3158
Fragments arranged by size:
3158 2509 1274
NheI G'CTAG_C
Cuts at: 135 135
Size: 6941
NspI r_CATG'y
Cuts at: 1902 3402 3994 4494 5401 1902
Size: 1500 592 500 907 3442
Fragments arranged by size:
3442 1500 907 592 500
PasI CC'CwG_GG
Cuts at: 382 756 1758 3858 382
Size: 374 1002 2100 3465
Fragments arranged by size:
3465 2100 1002 374
PciI A'CATG_T
Cuts at: 1898 5397 1898
Size: 3499 3442
PflMI CCAn_nnn'nTGG
Cuts at: 388 388
Size: 6941
PmeI GTTT'AAAC
Cuts at: 3940 3940
Size: 6941
PmlI CAC'GTG
Cuts at: 735 863 1188 2963 735
Size: 128 325 1775 4713
Fragments arranged by size:
4713 1775 325 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 778 810 958 990 1825 1857 6080 6112 778
Size: 32 148 32 835 32 4223 32 1607
Fragments arranged by size:
4223 1607 835 148 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 186 3234 186
Size: 3048 3893
PshAI GACnn'nnGTC
Cuts at: 1046 1046
Size: 6941
PspOMI G'GGCC_C
Cuts at: 438 1199 1828 2078 2478 2537 438
Size: 761 629 250 400 59 4842
Fragments arranged by size:
4842 761 629 400 250 59
PstI C_TGCA'G
Cuts at: 2876 3988 4673 2876
Size: 1112 685 5144
Fragments arranged by size:
5144 1112 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6941
PvuII CAG'CTG
Cuts at: 608 1822 2151 3712 4366 4726 608
Size: 1214 329 1561 654 360 2823
Fragments arranged by size:
2823 1561 1214 654 360 329
RsrII CG'GwC_CG
Cuts at: 2331 5136 2331
Size: 2805 4136
SacI G_AGCT'C
Cuts at: 365 911 3955 365
Size: 546 3044 3351
Fragments arranged by size:
3351 3044 546
SalI G'TCGA_C
Cuts at: 3978 3978
Size: 6941
SapI GCTCTTCn'nnn_
Cuts at: 2791 4964 5174 2791
Size: 2173 210 4558
Fragments arranged by size:
4558 2173 210
SbfI CC_TGCA'GG
Cuts at: 3988 3988
Size: 6941
ScaI AGT'ACT
Cuts at: 1570 2215 3055 6458 1570
Size: 645 840 3403 2053
Fragments arranged by size:
3403 2053 840 645
SfcI C'TryA_G
Cuts at: 33 2706 2872 3984 4669 5662 5853 33
Size: 2673 166 1112 685 993 191 1121
Fragments arranged by size:
2673 1121 1112 993 685 191 166
SfoI GGC'GCC
Cuts at: 847 1616 2069 2169 2610 4373 4621 847
Size: 769 453 100 441 1763 248 3167
Fragments arranged by size:
3167 1763 769 453 441 248 100
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6941
SgrAI Cr'CCGG_yG
Cuts at: 1430 2611 1430
Size: 1181 5760
SmaI CCC'GGG
Cuts at: 460 1484 1592 1652 2075 2383 3231 3963 460
Size: 1024 108 60 423 308 848 732 3438
Fragments arranged by size:
3438 1024 848 732 423 308 108 60
SmlI C'TyrA_G
Cuts at: 613 910 1249 1711 2392 3187 4096 5011 5503
Size: 297 339 462 681 795 909 915 492
Cuts at: 5503 5765 613
Size: 262 1789
Fragments arranged by size:
1789 915 909 795 681 492 462 339
297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6941
SphI G_CATG'C
Cuts at: 3994 4494 3994
Size: 500 6441
StuI AGG'CCT
Cuts at: 1057 3434 1057
Size: 2377 4564
StyI C'CwwG_G
Cuts at: 116 293 924 1052 2474 4075 4429 116
Size: 177 631 128 1422 1601 354 2628
Fragments arranged by size:
2628 1601 1422 631 354 177 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1747 1960 2348 4524 4657 5116 5325 1747
Size: 213 388 2176 133 459 209 3363
Fragments arranged by size:
3363 2176 459 388 213 209 133
TatI w'GTAC_w
Cuts at: 617 857 986 1031 1568 2213 3053 6456 617
Size: 240 129 45 537 645 840 3403 1102
Fragments arranged by size:
3403 1102 840 645 537 240 129 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1078 1110 1602 1634 3838 3870 6458 6490 1078
Size: 32 492 32 2204 32 2588 32 1529
Fragments arranged by size:
2588 2204 1529 492 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4738 4738
Size: 6941
XbaI T'CTAG_A
Cuts at: 3972 3972
Size: 6941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 1509 2050 4436 414
Size: 1095 541 2386 2919
Fragments arranged by size:
2919 2386 1095 541
XmaI C'CCGG_G
Cuts at: 458 1482 1590 1650 2073 2381 3229 3961 458
Size: 1024 108 60 423 308 848 732 3438
Fragments arranged by size:
3438 1024 848 732 423 308 108 60
XmnI GAAnn'nnTTC
Cuts at: 338 1141 1228 1285 2983 3127 338
Size: 803 87 57 1698 144 4152
Fragments arranged by size:
4152 1698 803 144 87 57
ZraI GAC'GTC
Cuts at: 1926 1926
Size: 6941
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AscI AvrII BaeI BfrBI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NotI NruI
NsiI PacI PfoI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI