Restriction map for
pF1KA1682
(Circular) (Six-base) MAPSORT of: pF1KA1682.seq Check: 2556 from: 1 to: 5354
pF1KA1682 5354 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2392 2392
Size: 5354
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 523 878 3179 3611 4358 4626 304
Size: 219 355 2301 432 747 268 1032
Fragments arranged by size:
2301 1032 747 432 355 268 219
AfeI AGC'GCT
Cuts at: 874 2032 874
Size: 1158 4196
AflII C'TTAA_G
Cuts at: 730 730
Size: 5354
AflIII A'CryG_T
Cuts at: 2706 3810 4787 2706
Size: 1104 977 3273
Fragments arranged by size:
3273 1104 977
AgeI A'CCGG_T
Cuts at: 3742 3742
Size: 5354
AlwNI CAG_nnn'CTG
Cuts at: 694 2279 2659 4226 694
Size: 1585 380 1567 1822
Fragments arranged by size:
1822 1585 1567 380
ApaI G_GGCC'C
Cuts at: 2009 2028 2009
Size: 19 5335
ApaLI G'TGCA_C
Cuts at: 1495 4124 1495
Size: 2629 2725
ApoI r'AATT_y
Cuts at: 191 1004 1105 1141 1519 2358 4707 4963 191
Size: 813 101 36 378 839 2349 256 582
Fragments arranged by size:
2349 839 813 582 378 256 101 36
AseI AT'TA_AT
Cuts at: 20 1904 20
Size: 1884 3470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 2374 2374
Size: 5354
AvrII C'CTAG_G
Cuts at: 2064 2337 2064
Size: 273 5081
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 142 2370 2784 3032 3067 142
Size: 2228 414 248 35 2429
Fragments arranged by size:
2429 2228 414 248 35
BanII G_rGCy'C
Cuts at: 1922 2009 2028 2368 3398 1922
Size: 87 19 340 1030 3878
Fragments arranged by size:
3878 1030 340 87 19
BbeI G_GCGC'C
Cuts at: 2788 3036 2788
Size: 248 5106
BbsI GAAGACnn'nnnn_
Cuts at: 1058 2306 1058
Size: 1248 4106
BbvCI CC'TCA_GC
Cuts at: 2177 2194 2177
Size: 17 5337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5320
BciVI GTATCCnnnnn_n'
Cuts at: 1629 3252 4013 5354 1629
Size: 1623 761 1341 1629
Fragments arranged by size:
1629 1623 1341 761
BclI T'GATC_A
Cuts at: 450 2874 450
Size: 2424 2930
BfrBI ATG'CAT
Cuts at: 1288 1288
Size: 5354
BglI GCCn_nnn'nGGC
Cuts at: 2173 2173
Size: 5354
BglII A'GATC_T
Cuts at: 2869 4906 2869
Size: 2037 3317
BlpI GC'TnA_GC
Cuts at: 2466 2466
Size: 5354
Bme1580I G_kGCm'C
Cuts at: 930 1499 2009 2028 2979 3072 4128 930
Size: 569 510 19 951 93 1056 2156
Fragments arranged by size:
2156 1056 951 569 510 93 19
BmrI ACTGGGnnnn_n'
Cuts at: 2566 2647 2982 5126 2566
Size: 81 335 2144 2794
Fragments arranged by size:
2794 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2224 2224
Size: 5354
Bpu10I CC'TnA_GC
Cuts at: 2177 2194 2177
Size: 17 5337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2530 3409 3901 4199 2530
Size: 879 492 298 3685
Fragments arranged by size:
3685 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5058 5058
Size: 5354
BsaAI yAC'GTr
Cuts at: 177 2707 177
Size: 2530 2824
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 2785 3033 3735 5231 2785
Size: 248 702 1496 2908
Fragments arranged by size:
2908 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2543 3064 3742 4016 4163 4574 2543
Size: 521 678 274 147 411 3323
Fragments arranged by size:
3323 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 612 642 612
Size: 30 5324
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1294 1462 1294
Size: 168 5186
BseYI C'CCAG_C
Cuts at: 111 2027 2779 3537 4114 4610 111
Size: 1916 752 758 577 496 855
Fragments arranged by size:
1916 855 758 752 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 659 889 1705 321
Size: 338 230 816 3970
Fragments arranged by size:
3970 816 338 230
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4150 104
Size: 2792 46 1208 1308
Fragments arranged by size:
2792 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1499 1922 2368 3146 3336 4128 1499
Size: 423 446 778 190 792 2725
Fragments arranged by size:
2725 792 778 446 423 190
BsiWI C'GTAC_G
Cuts at: 173 177 173
Size: 4 5350
BsmI GAATG_Cn'
Cuts at: 1290 1775 1290
Size: 485 4869
Bsp1286I G_dGCh'C
Cuts at: 930 1499 1922 2009 2028 2368 2979 3072 3146
Size: 569 423 87 19 340 611 93 74
Cuts at: 3146 3336 3398 4128 930
Size: 190 62 730 2156
Fragments arranged by size:
2156 730 611 569 423 340 190 93
87 74 62 19
BspEI T'CCGG_A
Cuts at: 4574 4574
Size: 5354
BspHI T'CATG_A
Cuts at: 4530 4530
Size: 5354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2404 2618 2920 3301 2404
Size: 214 302 381 4457
Fragments arranged by size:
4457 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1708 3266 1708
Size: 1558 3796
BsrFI r'CCGG_y
Cuts at: 3352 3533 3742 3352
Size: 181 209 4964
Fragments arranged by size:
4964 209 181
BssSI C'ACGA_G
Cuts at: 3625 3983 3625
Size: 358 4996
BstAPI GCAn_nnn'nTGC
Cuts at: 1494 2731 1494
Size: 1237 4117
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstYI r'GATC_y
Cuts at: 1 2379 2846 2869 3204 3450 4462 4548 4560
Size: 2378 467 23 335 246 1012 86 12
Cuts at: 4560 4906 1
Size: 346 449
Fragments arranged by size:
2378 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 185 185
Size: 5354
BtgI C'CryG_G
Cuts at: 1977 1977
Size: 5354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3304 3485 3648 3304
Size: 181 163 5010
Fragments arranged by size:
5010 181 163
BtsI GCAGTG_nn'
Cuts at: 531 791 946 1341 2597 531
Size: 260 155 395 1256 3288
Fragments arranged by size:
3288 1256 395 260 155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1366 1401 1366
Size: 35 5319
DraI TTT'AAA
Cuts at: 1110 2353 1110
Size: 1243 4111
DraIII CAC_nnn'GTG
Cuts at: 1670 1670
Size: 5354
DrdI GACnn_nn'nnGTC
Cuts at: 1545 3060 3918 1545
Size: 1515 858 2981
Fragments arranged by size:
2981 1515 858
EaeI y'GGCC_r
Cuts at: 1398 2073 2081 2309 2939 3113 3504 3531 4876
Size: 675 8 228 630 174 391 27 1345
Cuts at: 4876 1398
Size: 1876
Fragments arranged by size:
1876 1345 675 630 391 228 174 27
8
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 239 575 854 857 3377 3587 239
Size: 336 279 3 2520 210 2006
Fragments arranged by size:
2520 2006 336 279 210 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1420 3562 3872 4018 5164 1420
Size: 2142 310 146 1146 1610
Fragments arranged by size:
2142 1610 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1920 2366 1920
Size: 446 4908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 523 878 2224 3179 3611 4358 4626 304
Size: 219 355 1346 955 432 747 268 1032
Fragments arranged by size:
1346 1032 955 747 432 355 268 219
EcoO109I rG'GnC_Cy
Cuts at: 150 2005 2166 2181 2493 150
Size: 1855 161 15 312 3011
Fragments arranged by size:
3011 1855 312 161 15
EcoRI G'AATT_C
Cuts at: 1519 2358 1519
Size: 839 4515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 180 250 372 1011 1667 2280 3373 180
Size: 70 122 639 656 613 1093 2161
Fragments arranged by size:
2161 1093 656 639 613 122 70
FspI TGC'GCA
Cuts at: 3135 3135
Size: 5354
HaeII r_GCGC'y
Cuts at: 876 2034 2788 3036 4058 5037 876
Size: 1158 754 248 1022 979 1193
Fragments arranged by size:
1193 1158 1022 979 754 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 1273 1305 3196 3226 3228 3258 358
Size: 32 883 32 1891 30 2 30 2454
Fragments arranged by size:
2454 1891 883 32 32 30 30 2
HincII GTy'rAC
Cuts at: 795 2393 795
Size: 1598 3756
HindIII A'AGCT_T
Cuts at: 2285 2285
Size: 5354
KasI G'GCGC_C
Cuts at: 2784 3032 2784
Size: 248 5106
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1316 2023 4025 4209 1157
Size: 159 707 2002 184 2302
Fragments arranged by size:
2302 2002 707 184 159
MscI TGG'CCA
Cuts at: 1400 2075 2083 2311 3115 1400
Size: 675 8 228 804 3639
Fragments arranged by size:
3639 804 675 228 8
MslI CAynn'nnrTG
Cuts at: 3343 3470 3343
Size: 127 5227
MspA1I CmG'CkG
Cuts at: 258 1190 1491 1952 2465 2779 3139 4152 4397
Size: 932 301 461 513 314 360 1013 245
Cuts at: 4397 258
Size: 1215
Fragments arranged by size:
1215 1013 932 513 461 360 314 301
245
NaeI GCC'GGC
Cuts at: 3535 3535
Size: 5354
NarI GG'CG_CC
Cuts at: 2785 3033 2785
Size: 248 5106
NcoI C'CATG_G
Cuts at: 1977 1977
Size: 5354
NgoMIV G'CCGG_C
Cuts at: 3533 3533
Size: 5354
NsiI A_TGCA'T
Cuts at: 1290 1290
Size: 5354
NspI r_CATG'y
Cuts at: 2407 2907 3814 2407
Size: 500 907 3947
Fragments arranged by size:
3947 907 500
PacI TTA_AT'TAA
Cuts at: 1868 1868
Size: 5354
PciI A'CATG_T
Cuts at: 3810 3810
Size: 5354
PflMI CCAn_nnn'nTGG
Cuts at: 2279 2279
Size: 5354
PfoI T'CCnGG_A
Cuts at: 293 293
Size: 5354
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 5322
PspOMI G'GGCC_C
Cuts at: 2005 2024 2005
Size: 19 5335
PstI C_TGCA'G
Cuts at: 1082 1348 2401 3086 1082
Size: 266 1053 685 3350
Fragments arranged by size:
3350 1053 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 258 1190 1491 1952 2779 3139 258
Size: 932 301 461 827 360 2473
Fragments arranged by size:
2473 932 827 461 360 301
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 1922 2368 1922
Size: 446 4908
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 3377 3587 3377
Size: 210 5144
SbfI CC_TGCA'GG
Cuts at: 1082 2401 1082
Size: 1319 4035
ScaI AGT'ACT
Cuts at: 281 1137 4871 281
Size: 856 3734 764
Fragments arranged by size:
3734 856 764
SfcI C'TryA_G
Cuts at: 33 1078 1344 2397 3082 4075 4266 33
Size: 1045 266 1053 685 993 191 1121
Fragments arranged by size:
1121 1053 1045 993 685 266 191
SfoI GGC'GCC
Cuts at: 2786 3034 2786
Size: 248 5106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 730 2509 3424 3916 4178 730
Size: 1779 915 492 262 1906
Fragments arranged by size:
1906 1779 915 492 262
SnaBI TAC'GTA
Cuts at: 177 177
Size: 5354
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 2407 2907 2407
Size: 500 4854
StyI C'CwwG_G
Cuts at: 146 1977 2064 2337 2488 2842 146
Size: 1831 87 273 151 354 2658
Fragments arranged by size:
2658 1831 354 273 151 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1384 2937 3070 3529 3738 352
Size: 1032 1553 133 459 209 1968
Fragments arranged by size:
1968 1553 1032 459 209 133
TatI w'GTAC_w
Cuts at: 279 776 1135 4869 279
Size: 497 359 3734 764
Fragments arranged by size:
3734 764 497 359
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 588 620 837 869 4871 4903 588
Size: 32 217 32 4002 32 1039
Fragments arranged by size:
4002 1039 217 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3151 3151
Size: 5354
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 902 2065 2849 902
Size: 1163 784 3407
Fragments arranged by size:
3407 1163 784
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PacI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI AscI BaeI
BmgBI BmtI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PasI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI