Restriction map for
pF1KA1685
(Circular) (Six-base) MAPSORT of: pF1KA1685.seq Check: 6853 from: 1 to: 7418
pF1KA1685 7418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 485 1155 2463 2844 485
Size: 670 1308 381 5059
Fragments arranged by size:
5059 1308 670 381
AccI GT'mk_AC
Cuts at: 4456 4456
Size: 7418
Acc65I G'GTAC_C
Cuts at: 4434 4434
Size: 7418
AclI AA'CG_TT
Cuts at: 7258 7258
Size: 7418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1472 2696 3199 3292 3566 3596 5243 5675
Size: 381 1224 503 93 274 30 1647 432
Cuts at: 5675 6422 6690 1091
Size: 747 268 1819
Fragments arranged by size:
1819 1647 1224 747 503 432 381 274
268 93 30
AfeI AGC'GCT
Cuts at: 3083 3083
Size: 7418
AflIII A'CryG_T
Cuts at: 1863 4770 5874 6851 1863
Size: 2907 1104 977 2430
Fragments arranged by size:
2907 2430 1104 977
AgeI A'CCGG_T
Cuts at: 5806 5806
Size: 7418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3193 3193
Size: 7418
AlwNI CAG_nnn'CTG
Cuts at: 143 2128 2149 2185 2308 2620 2749 2836 3165
Size: 1985 21 36 123 312 129 87 329
Cuts at: 3165 4195 4723 6290 143
Size: 1030 528 1567 1271
Fragments arranged by size:
1985 1567 1271 1030 528 329 312 129
123 87 36 21
ApaI G_GGCC'C
Cuts at: 2287 2579 2287
Size: 292 7126
ApaLI G'TGCA_C
Cuts at: 6188 6188
Size: 7418
ApoI r'AATT_y
Cuts at: 206 1096 1172 3005 4422 6771 7027 206
Size: 890 76 1833 1417 2349 256 597
Fragments arranged by size:
2349 1833 1417 890 597 256 76
AseI AT'TA_AT
Cuts at: 20 20
Size: 7418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7418
AvaI C'yCGr_G
Cuts at: 2856 3964 4438 2856
Size: 1108 474 5836
Fragments arranged by size:
5836 1108 474
BamHI G'GATC_C
Cuts at: 4443 4443
Size: 7418
BanI G'GyrC_C
Cuts at: 2635 4434 4848 5096 5131 2635
Size: 1799 414 248 35 4922
Fragments arranged by size:
4922 1799 414 248 35
BanII G_rGCy'C
Cuts at: 1496 1795 2028 2287 2579 2678 3511 3704 4432
Size: 299 233 259 292 99 833 193 728
Cuts at: 4432 5462 1496
Size: 1030 3452
Fragments arranged by size:
3452 1030 833 728 299 292 259 233
193 99
BbeI G_GCGC'C
Cuts at: 4852 5100 4852
Size: 248 7170
BbsI GAAGACnn'nnnn_
Cuts at: 1808 3407 1808
Size: 1599 5819
BbvCI CC'TCA_GC
Cuts at: 1506 3238 1506
Size: 1732 5686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2847 2881 2847
Size: 34 7384
BciVI GTATCCnnnnn_n'
Cuts at: 2953 5316 6077 7418 2953
Size: 2363 761 1341 2953
Fragments arranged by size:
2953 2363 1341 761
BclI T'GATC_A
Cuts at: 965 1785 4097 4328 4938 965
Size: 820 2312 231 610 3445
Fragments arranged by size:
3445 2312 820 610 231
BglI GCCn_nnn'nGGC
Cuts at: 2551 2551
Size: 7418
BglII A'GATC_T
Cuts at: 862 4933 6970 862
Size: 4071 2037 1310
Fragments arranged by size:
4071 2037 1310
BlpI GC'TnA_GC
Cuts at: 1080 1528 3783 4530 1080
Size: 448 2255 747 3968
Fragments arranged by size:
3968 2255 747 448
Bme1580I G_kGCm'C
Cuts at: 1555 2287 2579 3476 4294 5043 5136 6192 1555
Size: 732 292 897 818 749 93 1056 2781
Fragments arranged by size:
2781 1056 897 818 749 732 292 93
BmrI ACTGGGnnnn_n'
Cuts at: 1289 1383 2358 2434 2573 4630 4711 5046 7190
Size: 94 975 76 139 2057 81 335 2144
Cuts at: 7190 1289
Size: 1517
Fragments arranged by size:
2144 2057 1517 975 335 139 94 81
76
BmtI G_CTAG'C
Cuts at: 3791 4217 3791
Size: 426 6992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5785 5785
Size: 7418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 780 1016 1962 2435 3231 282
Size: 498 236 946 473 796 4469
Fragments arranged by size:
4469 946 796 498 473 236
Bpu10I CC'TnA_GC
Cuts at: 1258 1506 3230 3238 1258
Size: 248 1724 8 5438
Fragments arranged by size:
5438 1724 248 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1789 1948 4594 5473 5965 6263 1789
Size: 159 2646 879 492 298 2944
Fragments arranged by size:
2944 2646 879 492 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 1908 1980 2019 2365 7122 1908
Size: 72 39 346 4757 2204
Fragments arranged by size:
4757 2204 346 72 39
BsaAI yAC'GTr
Cuts at: 1864 4771 1864
Size: 2907 4511
BsaBI GATnn'nnATC
Cuts at: 6 1565 4932 6969 6
Size: 1559 3367 2037 455
Fragments arranged by size:
3367 2037 1559 455
BsaHI Gr'CG_yC
Cuts at: 4849 5097 5799 7295 4849
Size: 248 702 1496 4972
Fragments arranged by size:
4972 1496 702 248
BsaWI w'CCGG_w
Cuts at: 927 4607 5128 5806 6080 6227 6638 927
Size: 3680 521 678 274 147 411 1707
Fragments arranged by size:
3680 1707 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 280 310 778 808 1275 1305 280
Size: 30 468 30 467 30 6393
Fragments arranged by size:
6393 468 467 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 251 663 2255 3712 251
Size: 412 1592 1457 3957
Fragments arranged by size:
3957 1592 1457 412
BseYI C'CCAG_C
Cuts at: 1320 1847 3165 3429 4231 4843 5601 6178 6674
Size: 527 1318 264 802 612 758 577 496
Cuts at: 6674 1320
Size: 2064
Fragments arranged by size:
2064 1318 802 758 612 577 527 496
264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2675 1486
Size: 1189 6229
BsiEI CG_ry'CG
Cuts at: 104 3504 4960 5006 6214 104
Size: 3400 1456 46 1208 1308
Fragments arranged by size:
3400 1456 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 963 2421 2678 2863 4432 5210 5400 6192 963
Size: 1458 257 185 1569 778 190 792 2189
Fragments arranged by size:
2189 1569 1458 792 778 257 190 185
BsmI GAATG_Cn'
Cuts at: 282 1535 282
Size: 1253 6165
BsmBI CGTCTCn'nnnn_
Cuts at: 4398 4398
Size: 7418
Bsp1286I G_dGCh'C
Cuts at: 963 1496 1555 1795 2028 2287 2421 2579 2678
Size: 533 59 240 233 259 134 158 99
Cuts at: 2678 2863 3476 3511 3704 4294 4432 5043 5136
Size: 185 613 35 193 590 138 611 93
Cuts at: 5136 5210 5400 5462 6192 963
Size: 74 190 62 730 2189
Fragments arranged by size:
2189 730 613 611 590 533 259 240
233 193 190 185 158 138 134 99
93 74 62 59 35
BspEI T'CCGG_A
Cuts at: 6638 6638
Size: 7418
BspHI T'CATG_A
Cuts at: 6594 6594
Size: 7418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 485 550 735 744 1155 2001 2463 2674 2844
Size: 65 185 9 411 846 462 211 170
Cuts at: 2844 3468 4468 4682 4984 5365 485
Size: 624 1000 214 302 381 2538
Fragments arranged by size:
2538 1000 846 624 462 411 381 302
214 211 185 170 65 9
BsrBI CCG'CTC
Cuts at: 50 1738 5710 5764 7248 7413 50
Size: 1688 3972 54 1484 165 55
Fragments arranged by size:
3972 1688 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 287 655 1411 3470 4170 5330 287
Size: 368 756 2059 700 1160 2375
Fragments arranged by size:
2375 2059 1160 756 700 368
BsrFI r'CCGG_y
Cuts at: 5416 5597 5806 5416
Size: 181 209 7028
Fragments arranged by size:
7028 209 181
BssSI C'ACGA_G
Cuts at: 5689 6047 5689
Size: 358 7060
BstAPI GCAn_nnn'nTGC
Cuts at: 1014 2128 2149 3009 4795 1014
Size: 1114 21 860 1786 3637
Fragments arranged by size:
3637 1786 1114 860 21
BstBI TT'CG_AA
Cuts at: 4300 5779 4300
Size: 1479 5939
BstXI CCAn_nnnn'nTGG
Cuts at: 3721 3721
Size: 7418
BstYI r'GATC_y
Cuts at: 1 862 1566 1967 3302 4443 4910 4933 5268
Size: 861 704 401 1335 1141 467 23 335
Cuts at: 5268 5514 6526 6612 6624 6970 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1335 1141 1012 861 704 467 449 401
346 335 246 86 23 12
BtgI C'CryG_G
Cuts at: 1210 2996 4239 1210
Size: 1786 1243 4389
Fragments arranged by size:
4389 1786 1243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1661 1991 5368 5549 5712 1661
Size: 330 3377 181 163 3367
Fragments arranged by size:
3377 3367 330 181 163
BtsI GCAGTG_nn'
Cuts at: 417 465 3941 4661 417
Size: 48 3476 720 3174
Fragments arranged by size:
3476 3174 720 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4326 4361 4326
Size: 35 7383
DraI TTT'AAA
Cuts at: 2906 4417 2906
Size: 1511 5907
DrdI GACnn_nn'nnGTC
Cuts at: 1049 5124 5982 1049
Size: 4075 858 2485
Fragments arranged by size:
4075 2485 858
EaeI y'GGCC_r
Cuts at: 3501 4242 5003 5177 5568 5595 6940 3501
Size: 741 761 174 391 27 1345 3979
Fragments arranged by size:
3979 1345 761 741 391 174 27
EagI C'GGCC_G
Cuts at: 3501 5003 3501
Size: 1502 5916
EarI CTCTTCn'nnn_
Cuts at: 302 1436 1826 1849 4268 5441 5651 302
Size: 1134 390 23 2419 1173 210 2069
Fragments arranged by size:
2419 2069 1173 1134 390 210 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 5626 5936 6082 7228 163
Size: 5463 310 146 1146 353
Fragments arranged by size:
5463 1146 353 310 146
EcoICRI GAG'CTC
Cuts at: 2676 4430 2676
Size: 1754 5664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 780 1016 1091 1472 1962 2435 2696 3199
Size: 498 236 75 381 490 473 261 503
Cuts at: 3199 3231 3292 3566 3596 5243 5675 6422 6690
Size: 32 61 274 30 1647 432 747 268
Cuts at: 6690 282
Size: 1010
Fragments arranged by size:
1647 1010 747 503 498 490 473 432
381 274 268 261 236 75 61 32
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2189
Size: 7418
EcoO109I rG'GnC_Cy
Cuts at: 140 1836 2182 2283 2284 2575 2617 2686 3209
Size: 1696 346 101 1 291 42 69 523
Cuts at: 3209 3527 3745 4557 140
Size: 318 218 812 3001
Fragments arranged by size:
3001 1696 812 523 346 318 291 218
101 69 42 1
EcoRI G'AATT_C
Cuts at: 1096 1172 4422 1096
Size: 76 3250 4092
Fragments arranged by size:
4092 3250 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 226 272 5437 183
Size: 43 46 5165 2164
Fragments arranged by size:
5165 2164 46 43
FspI TGC'GCA
Cuts at: 5199 5199
Size: 7418
HaeII r_GCGC'y
Cuts at: 616 3085 4352 4852 5100 6122 7101 616
Size: 2469 1267 500 248 1022 979 933
Fragments arranged by size:
2469 1267 1022 979 933 500 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 246 278 406 438 950 982 1275 1307 3124
Size: 32 128 32 512 32 293 32 1817
Cuts at: 3124 3156 3324 3356 3664 3696 4082 4114 4117
Size: 32 168 32 308 32 386 32 3
Cuts at: 4117 4149 4165 4197 5260 5290 5292 5322 246
Size: 32 16 32 1063 30 2 30 2342
Fragments arranged by size:
2342 1817 1063 512 386 308 293 168
128 32 32 32 32 32 32 32
32 32 32 30 30 16 3 2
HincII GTy'rAC
Cuts at: 4457 4457
Size: 7418
HindIII A'AGCT_T
Cuts at: 2800 2800
Size: 7418
KasI G'GCGC_C
Cuts at: 4848 5096 4848
Size: 248 7170
KpnI G_GTAC'C
Cuts at: 4438 4438
Size: 7418
MluI A'CGCG_T
Cuts at: 6851 6851
Size: 7418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2145 2473 2635 6089 6273 701
Size: 1444 328 162 3454 184 1846
Fragments arranged by size:
3454 1846 1444 328 184 162
MscI TGG'CCA
Cuts at: 4244 5179 4244
Size: 935 6483
MslI CAynn'nnrTG
Cuts at: 1345 4330 5407 5534 1345
Size: 2985 1077 127 3229
Fragments arranged by size:
3229 2985 1077 127
MspA1I CmG'CkG
Cuts at: 1324 2128 2134 2146 2659 2973 3165 3396 4529
Size: 804 6 12 513 314 192 231 1133
Cuts at: 4529 4843 5203 6216 6461 1324
Size: 314 360 1013 245 2281
Fragments arranged by size:
2281 1133 1013 804 513 360 314 314
245 231 192 12 6
NaeI GCC'GGC
Cuts at: 5599 5599
Size: 7418
NarI GG'CG_CC
Cuts at: 4849 5097 4849
Size: 248 7170
NcoI C'CATG_G
Cuts at: 1210 2996 4239 1210
Size: 1786 1243 4389
Fragments arranged by size:
4389 1786 1243
NgoMIV G'CCGG_C
Cuts at: 5597 5597
Size: 7418
NheI G'CTAG_C
Cuts at: 3787 4213 3787
Size: 426 6992
NspI r_CATG'y
Cuts at: 272 2474 4471 4971 5878 272
Size: 2202 1997 500 907 1812
Fragments arranged by size:
2202 1997 1812 907 500
PasI CC'CwG_GG
Cuts at: 2021 2191 2021
Size: 170 7248
PciI A'CATG_T
Cuts at: 5874 5874
Size: 7418
PflMI CCAn_nnn'nTGG
Cuts at: 2185 2197 2185
Size: 12 7406
PfoI T'CCnGG_A
Cuts at: 993 2178 2208 993
Size: 1185 30 6203
Fragments arranged by size:
6203 1185 30
PmeI GTTT'AAAC
Cuts at: 4417 4417
Size: 7418
PmlI CAC'GTG
Cuts at: 1864 1864
Size: 7418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6557 6589 6557
Size: 32 7386
PpuMI rG'GwC_Cy
Cuts at: 140 2182 2617 2686 3527 3745 140
Size: 2042 435 69 841 218 3813
Fragments arranged by size:
3813 2042 841 435 218 69
PsiI TTA'TAA
Cuts at: 1643 1643
Size: 7418
PspOMI G'GGCC_C
Cuts at: 2283 2575 2283
Size: 292 7126
PspXI vC'TCGA_Gb
Cuts at: 2856 2856
Size: 7418
PstI C_TGCA'G
Cuts at: 2154 4002 4465 5150 2154
Size: 1848 463 685 4422
Fragments arranged by size:
4422 1848 685 463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7418
PvuII CAG'CTG
Cuts at: 1324 2128 2146 2659 2973 3165 3396 4843 5203
Size: 804 18 513 314 192 231 1447 360
Cuts at: 5203 1324
Size: 3539
Fragments arranged by size:
3539 1447 804 513 360 314 231 192
18
RsrII CG'GwC_CG
Cuts at: 5613 5613
Size: 7418
SacI G_AGCT'C
Cuts at: 2678 4432 2678
Size: 1754 5664
SalI G'TCGA_C
Cuts at: 4455 4455
Size: 7418
SapI GCTCTTCn'nnn_
Cuts at: 5441 5651 5441
Size: 210 7208
SbfI CC_TGCA'GG
Cuts at: 4465 4465
Size: 7418
ScaI AGT'ACT
Cuts at: 3663 6935 3663
Size: 3272 4146
SexAI A'CCwGG_T
Cuts at: 4143 4143
Size: 7418
SfcI C'TryA_G
Cuts at: 33 2150 2425 2828 3998 4086 4461 5146 6139
Size: 2117 275 403 1170 88 375 685 993
Cuts at: 6139 6330 33
Size: 191 1121
Fragments arranged by size:
2117 1170 1121 993 685 403 375 275
191 88
SfoI GGC'GCC
Cuts at: 4850 5098 4850
Size: 248 7170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7418
SmaI CCC'GGG
Cuts at: 4440 4440
Size: 7418
SmlI C'TyrA_G
Cuts at: 1804 1963 2856 4573 5488 5980 6242 1804
Size: 159 893 1717 915 492 262 2980
Fragments arranged by size:
2980 1717 915 893 492 262 159
SpeI A'CTAG_T
Cuts at: 67 2047 67
Size: 1980 5438
SphI G_CATG'C
Cuts at: 272 2474 4471 4971 272
Size: 2202 1997 500 2719
Fragments arranged by size:
2719 2202 1997 500
SspI AAT'ATT
Cuts at: 3489 3489
Size: 7418
StuI AGG'CCT
Cuts at: 1357 1357
Size: 7418
StyI C'CwwG_G
Cuts at: 1116 1210 1455 2996 3205 4239 4552 4906 1116
Size: 94 245 1541 209 1034 313 354 3628
Fragments arranged by size:
3628 1541 1034 354 313 245 209 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 561 1840 4027 4084 5001 5134 5593 5802
Size: 357 1279 2187 57 917 133 459 209
Cuts at: 5802 204
Size: 1820
Fragments arranged by size:
2187 1820 1279 917 459 357 209 133
57
TatI w'GTAC_w
Cuts at: 3661 6933 3661
Size: 3272 4146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 280 312 6935 6967 280
Size: 32 6623 32 731
Fragments arranged by size:
6623 731 32 32
Tth111I GACn'n_nGTC
Cuts at: 2204 2263 5215 2204
Size: 59 2952 4407
Fragments arranged by size:
4407 2952 59
XbaI T'CTAG_A
Cuts at: 4449 4449
Size: 7418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2295 3462 4913 2295
Size: 1167 1451 4800
Fragments arranged by size:
4800 1451 1167
XhoI C'TCGA_G
Cuts at: 2856 2856
Size: 7418
XmaI C'CCGG_G
Cuts at: 4438 4438
Size: 7418
XmnI GAAnn'nnTTC
Cuts at: 209 853 2801 209
Size: 644 1948 4826
Fragments arranged by size:
4826 1948 644
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BfrBI BmgBI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI NsiI PacI PshAI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SwaI ZraI