Restriction map for
pF1KA1687
(Circular) (Six-base) MAPSORT of: pF1KA1687.seq Check: 7189 from: 1 to: 5267
pF1KA1687 5267 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 2305 2305
Size: 5267
Acc65I G'GTAC_C
Cuts at: 2283 2283
Size: 5267
AclI AA'CG_TT
Cuts at: 5107 5107
Size: 5267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 313 569 593 3092 3524 4271 4539 199
Size: 114 256 24 2499 432 747 268 927
Fragments arranged by size:
2499 927 747 432 268 256 114 24
AflIII A'CryG_T
Cuts at: 2619 3723 4700 2619
Size: 1104 977 3186
Fragments arranged by size:
3186 1104 977
AgeI A'CCGG_T
Cuts at: 3655 3655
Size: 5267
AlwNI CAG_nnn'CTG
Cuts at: 118 718 1248 2572 4139 118
Size: 600 530 1324 1567 1246
Fragments arranged by size:
1567 1324 1246 600 530
ApaLI G'TGCA_C
Cuts at: 4037 4037
Size: 5267
ApoI r'AATT_y
Cuts at: 375 447 807 1063 1088 2271 4620 4876 375
Size: 72 360 256 25 1183 2349 256 766
Fragments arranged by size:
2349 1183 766 360 256 256 72 25
AseI AT'TA_AT
Cuts at: 20 1118 20
Size: 1098 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
AvaI C'yCGr_G
Cuts at: 275 2084 2287 275
Size: 1809 203 3255
Fragments arranged by size:
3255 1809 203
BamHI G'GATC_C
Cuts at: 2292 2292
Size: 5267
BanI G'GyrC_C
Cuts at: 330 1486 2067 2283 2697 2945 2980 330
Size: 1156 581 216 414 248 35 2617
Fragments arranged by size:
2617 1156 581 414 248 216 35
BanII G_rGCy'C
Cuts at: 2281 3311 2281
Size: 1030 4237
BbeI G_GCGC'C
Cuts at: 2701 2949 2701
Size: 248 5019
BbsI GAAGACnn'nnnn_
Cuts at: 205 557 880 205
Size: 352 323 4592
Fragments arranged by size:
4592 352 323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 629 1345 1379 595
Size: 34 716 34 4483
Fragments arranged by size:
4483 716 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3165 3926 5267 3165
Size: 761 1341 3165
Fragments arranged by size:
3165 1341 761
BclI T'GATC_A
Cuts at: 2787 2787
Size: 5267
BglII A'GATC_T
Cuts at: 568 1254 2782 4819 568
Size: 686 1528 2037 1016
Fragments arranged by size:
2037 1528 1016 686
BlpI GC'TnA_GC
Cuts at: 2379 2379
Size: 5267
Bme1580I G_kGCm'C
Cuts at: 1398 2107 2892 2985 4041 1398
Size: 709 785 93 1056 2624
Fragments arranged by size:
2624 1056 785 709 93
BmgBI CAC'GTC
Cuts at: 1840 1927 1840
Size: 87 5180
BmrI ACTGGGnnnn_n'
Cuts at: 1207 2479 2560 2895 5039 1207
Size: 1272 81 335 2144 1435
Fragments arranged by size:
2144 1435 1272 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 290 2099 2137 3634 290
Size: 1809 38 1497 1923
Fragments arranged by size:
1923 1809 1497 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 1992 183
Size: 1809 3458
Bpu10I CC'TnA_GC
Cuts at: 960 1653 960
Size: 693 4574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 2443 3322 3814 4112 1309
Size: 1134 879 492 298 2464
Fragments arranged by size:
2464 1134 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1129 4971 1129
Size: 3842 1425
BsaAI yAC'GTr
Cuts at: 1764 2127 2620 1764
Size: 363 493 4411
Fragments arranged by size:
4411 493 363
BsaBI GATnn'nnATC
Cuts at: 6 2781 4818 6
Size: 2775 2037 455
Fragments arranged by size:
2775 2037 455
BsaHI Gr'CG_yC
Cuts at: 2698 2946 3648 5144 2698
Size: 248 702 1496 2821
Fragments arranged by size:
2821 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2456 2977 3655 3929 4076 4487 2456
Size: 521 678 274 147 411 3236
Fragments arranged by size:
3236 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1508 1538 1890 1920 1990 2020 1508
Size: 30 352 30 70 30 4755
Fragments arranged by size:
4755 352 70 30 30 30
BseYI C'CCAG_C
Cuts at: 687 2692 3450 4027 4523 687
Size: 2005 758 577 496 1431
Fragments arranged by size:
2005 1431 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1125 1198 838
Size: 287 73 4907
Fragments arranged by size:
4907 287 73
BsiEI CG_ry'CG
Cuts at: 104 2809 2855 4063 104
Size: 2705 46 1208 1308
Fragments arranged by size:
2705 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1911 2281 3059 3249 4041 1911
Size: 370 778 190 792 3137
Fragments arranged by size:
3137 792 778 370 190
BsmI GAATG_Cn'
Cuts at: 564 564
Size: 5267
BsmBI CGTCTCn'nnnn_
Cuts at: 1553 1553
Size: 5267
Bsp1286I G_dGCh'C
Cuts at: 1398 1911 2107 2281 2892 2985 3059 3249 3311
Size: 513 196 174 611 93 74 190 62
Cuts at: 3311 4041 1398
Size: 730 2624
Fragments arranged by size:
2624 730 611 513 196 190 174 93
74 62
BspEI T'CCGG_A
Cuts at: 4487 4487
Size: 5267
BspHI T'CATG_A
Cuts at: 1698 4443 1698
Size: 2745 2522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 248 2317 2531 2833 3214 248
Size: 2069 214 302 381 2301
Fragments arranged by size:
2301 2069 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3559 3613 5097 5262 50
Size: 3509 54 1484 165 55
Fragments arranged by size:
3509 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 740 2001 3179 740
Size: 1261 1178 2828
Fragments arranged by size:
2828 1261 1178
BsrFI r'CCGG_y
Cuts at: 345 3265 3446 3655 345
Size: 2920 181 209 1957
Fragments arranged by size:
2920 1957 209 181
BsrGI T'GTAC_A
Cuts at: 994 994
Size: 5267
BssSI C'ACGA_G
Cuts at: 3538 3896 3538
Size: 358 4909
BstAPI GCAn_nnn'nTGC
Cuts at: 1176 1402 2644 1176
Size: 226 1242 3799
Fragments arranged by size:
3799 1242 226
BstBI TT'CG_AA
Cuts at: 3628 3628
Size: 5267
BstYI r'GATC_y
Cuts at: 1 146 568 792 1254 1345 2292 2759 2782
Size: 145 422 224 462 91 947 467 23
Cuts at: 2782 3117 3363 4375 4461 4473 4819 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 947 467 462 449 422 346 335
246 224 145 91 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 151 1459 1742 151
Size: 1308 283 3676
Fragments arranged by size:
3676 1308 283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3217 3398 3561 3217
Size: 181 163 4923
Fragments arranged by size:
4923 181 163
BtsI GCAGTG_nn'
Cuts at: 719 1113 1165 2510 719
Size: 394 52 1345 3476
Fragments arranged by size:
3476 1345 394 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1908 1943 1908
Size: 35 5232
DraI TTT'AAA
Cuts at: 1568 2266 1568
Size: 698 4569
DrdI GACnn_nn'nnGTC
Cuts at: 2973 3831 2973
Size: 858 4409
EaeI y'GGCC_r
Cuts at: 1497 2852 3026 3417 3444 4789 1497
Size: 1355 174 391 27 1345 1975
Fragments arranged by size:
1975 1355 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2852 2852
Size: 5267
EarI CTCTTCn'nnn_
Cuts at: 259 569 617 764 1127 1551 2220 3290 3500
Size: 310 48 147 363 424 669 1070 210
Cuts at: 3500 259
Size: 2026
Fragments arranged by size:
2026 1070 669 424 363 310 210 147
48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3475 3785 3931 5077 3475
Size: 310 146 1146 3665
Fragments arranged by size:
3665 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2279 2279
Size: 5267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 199 313 569 593 1992 3092 3524 4271
Size: 16 114 256 24 1399 1100 432 747
Cuts at: 4271 4539 183
Size: 268 911
Fragments arranged by size:
1399 1100 911 747 432 268 256 114
24 16
EcoNI CCTnn'n_nnAGG
Cuts at: 185 185
Size: 5267
EcoO109I rG'GnC_Cy
Cuts at: 242 1737 2406 242
Size: 1495 669 3103
Fragments arranged by size:
3103 1495 669
EcoRI G'AATT_C
Cuts at: 1063 2271 1063
Size: 1208 4059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 5267
FspI TGC'GCA
Cuts at: 3048 3048
Size: 5267
HaeII r_GCGC'y
Cuts at: 2701 2949 3971 4950 2701
Size: 248 1022 979 3018
Fragments arranged by size:
3018 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 904 936 1330 1362 2041 2073 3109 3139 3141
Size: 32 394 32 679 32 1036 30 2
Cuts at: 3141 3171 904
Size: 30 3000
Fragments arranged by size:
3000 1036 679 394 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1637 1778 2060 2217 2306 1637
Size: 141 282 157 89 4598
Fragments arranged by size:
4598 282 157 141 89
HindIII A'AGCT_T
Cuts at: 461 461
Size: 5267
HpaI GTT'AAC
Cuts at: 2217 2217
Size: 5267
KasI G'GCGC_C
Cuts at: 2697 2945 2697
Size: 248 5019
KpnI G_GTAC'C
Cuts at: 2287 2287
Size: 5267
MfeI C'AATT_G
Cuts at: 844 844
Size: 5267
MluI A'CGCG_T
Cuts at: 4700 4700
Size: 5267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2112 3938 4122 2021
Size: 91 1826 184 3166
Fragments arranged by size:
3166 1826 184 91
MscI TGG'CCA
Cuts at: 3028 3028
Size: 5267
MslI CAynn'nnrTG
Cuts at: 112 1120 3256 3383 112
Size: 1008 2136 127 1996
Fragments arranged by size:
2136 1996 1008 127
MspA1I CmG'CkG
Cuts at: 897 2378 2692 3052 4065 4310 897
Size: 1481 314 360 1013 245 1854
Fragments arranged by size:
1854 1481 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3448 3448
Size: 5267
NarI GG'CG_CC
Cuts at: 2698 2946 2698
Size: 248 5019
NdeI CA'TA_TG
Cuts at: 2175 2175
Size: 5267
NgoMIV G'CCGG_C
Cuts at: 3446 3446
Size: 5267
NspI r_CATG'y
Cuts at: 2240 2320 2820 3727 2240
Size: 80 500 907 3780
Fragments arranged by size:
3780 907 500 80
PciI A'CATG_T
Cuts at: 3723 3723
Size: 5267
PflMI CCAn_nnn'nTGG
Cuts at: 699 1472 699
Size: 773 4494
PmeI GTTT'AAAC
Cuts at: 2266 2266
Size: 5267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 4406
Size: 32 5235
PpuMI rG'GwC_Cy
Cuts at: 242 1737 242
Size: 1495 3772
PshAI GACnn'nnGTC
Cuts at: 904 904
Size: 5267
PsiI TTA'TAA
Cuts at: 592 592
Size: 5267
PstI C_TGCA'G
Cuts at: 882 896 2314 2999 882
Size: 14 1418 685 3150
Fragments arranged by size:
3150 1418 685 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5267
PvuII CAG'CTG
Cuts at: 897 2692 3052 897
Size: 1795 360 3112
Fragments arranged by size:
3112 1795 360
RsrII CG'GwC_CG
Cuts at: 3462 3462
Size: 5267
SacI G_AGCT'C
Cuts at: 2281 2281
Size: 5267
SalI G'TCGA_C
Cuts at: 2304 2304
Size: 5267
SanDI GG'GwC_CC
Cuts at: 242 1737 242
Size: 1495 3772
SapI GCTCTTCn'nnn_
Cuts at: 259 569 2220 3290 3500 259
Size: 310 1651 1070 210 2026
Fragments arranged by size:
2026 1651 1070 310 210
SbfI CC_TGCA'GG
Cuts at: 2314 2314
Size: 5267
ScaI AGT'ACT
Cuts at: 4784 4784
Size: 5267
SfcI C'TryA_G
Cuts at: 33 521 878 892 1406 1688 1724 2310 2995
Size: 488 357 14 514 282 36 586 685
Cuts at: 2995 3988 4179 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 586 514 488 357 282
191 36 14
SfoI GGC'GCC
Cuts at: 2699 2947 2699
Size: 248 5019
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
SmaI CCC'GGG
Cuts at: 2289 2289
Size: 5267
SmlI C'TyrA_G
Cuts at: 1288 2084 2422 3337 3829 4091 1288
Size: 796 338 915 492 262 2464
Fragments arranged by size:
2464 915 796 492 338 262
SnaBI TAC'GTA
Cuts at: 1764 1764
Size: 5267
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5267
SphI G_CATG'C
Cuts at: 2320 2820 2320
Size: 500 4767
StyI C'CwwG_G
Cuts at: 812 987 2401 2755 812
Size: 175 1414 354 3324
Fragments arranged by size:
3324 1414 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1152 2850 2983 3442 3651 1152
Size: 1698 133 459 209 2768
Fragments arranged by size:
2768 1698 459 209 133
TatI w'GTAC_w
Cuts at: 994 4782 994
Size: 3788 1479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1890 1922 4784 4816 1890
Size: 32 2862 32 2341
Fragments arranged by size:
2862 2341 32 32
Tth111I GACn'n_nGTC
Cuts at: 3064 3064
Size: 5267
XbaI T'CTAG_A
Cuts at: 2298 2298
Size: 5267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2762 2762
Size: 5267
XhoI C'TCGA_G
Cuts at: 2084 2084
Size: 5267
XmaI C'CCGG_G
Cuts at: 2287 2287
Size: 5267
XmnI GAAnn'nnTTC
Cuts at: 451 451
Size: 5267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
BseRI BsiWI BssHII BstEII BstXI BstZ17I BtgI ClaI
DraIII EcoRV FseI FspAI NcoI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI SspI StuI SwaI
ZraI