Restriction map for
pF1KA1692
(Circular) (Six-base) MAPSORT of: pF1KA1692.seq Check: 6364 from: 1 to: 5612
pF1KA1692 5612 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1888 1888
Size: 5612
AccI GT'mk_AC
Cuts at: 2650 2650
Size: 5612
Acc65I G'GTAC_C
Cuts at: 2628 2628
Size: 5612
AclI AA'CG_TT
Cuts at: 5452 5452
Size: 5612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1610 1909 2160 3437 3869 4222 4884 227
Size: 1383 299 251 1277 432 353 662 955
Fragments arranged by size:
1383 1277 955 662 432 353 299 251
AflIII A'CryG_T
Cuts at: 2165 2964 4764 5045 2165
Size: 799 1800 281 2732
Fragments arranged by size:
2732 1800 799 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2217 2217
Size: 5612
AlwNI CAG_nnn'CTG
Cuts at: 1028 1600 1678 1822 1986 2917 4355 1028
Size: 572 78 144 164 931 1438 2285
Fragments arranged by size:
2285 1438 931 572 164 144 78
ApaI G_GGCC'C
Cuts at: 1138 1138
Size: 5612
ApaLI G'TGCA_C
Cuts at: 4450 4450
Size: 5612
ApoI r'AATT_y
Cuts at: 511 1258 1665 2616 4965 5221 511
Size: 747 407 951 2349 256 902
Fragments arranged by size:
2349 951 902 747 407 256
AseI AT'TA_AT
Cuts at: 20 695 20
Size: 675 4937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
AvaI C'yCGr_G
Cuts at: 799 2462 2632 799
Size: 1663 170 3779
Fragments arranged by size:
3779 1663 170
BamHI G'GATC_C
Cuts at: 1955 2637 1955
Size: 682 4930
BanI G'GyrC_C
Cuts at: 2628 3042 3290 3325 2628
Size: 414 248 35 4915
Fragments arranged by size:
4915 414 248 35
BanII G_rGCy'C
Cuts at: 362 1138 2040 2626 3656 362
Size: 776 902 586 1030 2318
Fragments arranged by size:
2318 1030 902 776 586
BbeI G_GCGC'C
Cuts at: 3046 3294 3046
Size: 248 5364
BbsI GAAGACnn'nnnn_
Cuts at: 200 416 1313 1352 1616 1847 2056 200
Size: 216 897 39 264 231 209 3756
Fragments arranged by size:
3756 897 264 231 216 209 39
BbvCI CC'TCA_GC
Cuts at: 686 1771 1946 686
Size: 1085 175 4352
Fragments arranged by size:
4352 1085 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5578
BciVI GTATCCnnnnn_n'
Cuts at: 3510 4566 5612 3510
Size: 1056 1046 3510
Fragments arranged by size:
3510 1056 1046
BclI T'GATC_A
Cuts at: 600 1824 3132 600
Size: 1224 1308 3080
Fragments arranged by size:
3080 1308 1224
BfrBI ATG'CAT
Cuts at: 111 111
Size: 5612
BglII A'GATC_T
Cuts at: 144 588 3127 5164 144
Size: 444 2539 2037 592
Fragments arranged by size:
2539 2037 592 444
BlpI GC'TnA_GC
Cuts at: 2724 2724
Size: 5612
Bme1580I G_kGCm'C
Cuts at: 1138 3237 3330 4454 1138
Size: 2099 93 1124 2296
Fragments arranged by size:
2296 2099 1124 93
BmgBI CAC'GTC
Cuts at: 2166 2166
Size: 5612
BmrI ACTGGGnnnn_n'
Cuts at: 472 2824 2905 3240 5384 472
Size: 2352 81 335 2144 700
Fragments arranged by size:
2352 2144 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3979 3979
Size: 5612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1691 2524 1114
Size: 577 833 4202
Fragments arranged by size:
4202 833 577
Bpu10I CC'TnA_GC
Cuts at: 686 1771 1946 686
Size: 1085 175 4352
Fragments arranged by size:
4352 1085 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2788 3667 4381 4679 2788
Size: 879 714 298 3721
Fragments arranged by size:
3721 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 313 384 5316 313
Size: 71 4932 609
Fragments arranged by size:
4932 609 71
BsaAI yAC'GTr
Cuts at: 2965 2965
Size: 5612
BsaBI GATnn'nnATC
Cuts at: 6 1960 3126 5163 6
Size: 1954 1166 2037 455
Fragments arranged by size:
2037 1954 1166 455
BsaHI Gr'CG_yC
Cuts at: 3043 3291 3993 5489 3043
Size: 248 702 1496 3166
Fragments arranged by size:
3166 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2801 3322 4000 4411 4558 4832 2801
Size: 521 678 411 147 274 3581
Fragments arranged by size:
3581 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 1057 1087 1105 1135 2086 2116 154
Size: 30 873 30 18 30 951 30 3650
Fragments arranged by size:
3650 951 873 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 407 1113 407
Size: 706 4906
BseYI C'CCAG_C
Cuts at: 157 1614 3037 3795 4460 4868 157
Size: 1457 1423 758 665 408 901
Fragments arranged by size:
1457 1423 901 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 561
Size: 5612
BsiEI CG_ry'CG
Cuts at: 104 3154 3200 4430 104
Size: 3050 46 1230 1286
Fragments arranged by size:
3050 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2040 2070 2626 3404 3594 4454 2040
Size: 30 556 778 190 860 3198
Fragments arranged by size:
3198 860 778 556 190 30
BsmI GAATG_Cn'
Cuts at: 1068 2529 1068
Size: 1461 4151
Bsp1286I G_dGCh'C
Cuts at: 362 1138 2040 2070 2626 3237 3330 3404 3594
Size: 776 902 30 556 611 93 74 190
Cuts at: 3594 3656 4454 362
Size: 62 798 1520
Fragments arranged by size:
1520 902 798 776 611 556 190 93
74 62 30
BspHI T'CATG_A
Cuts at: 443 4044 443
Size: 3601 2011
BspMI ACCTGCnnnn'nnnn_
Cuts at: 672 1888 2662 2876 3178 3559 672
Size: 1216 774 214 302 381 2725
Fragments arranged by size:
2725 1216 774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2453 3904 3958 5442 5607 50
Size: 2403 1451 54 1484 165 55
Fragments arranged by size:
2403 1484 1451 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 1181 2206 3524 500
Size: 681 1025 1318 2588
Fragments arranged by size:
2588 1318 1025 681
BsrFI r'CCGG_y
Cuts at: 3610 3791 3610
Size: 181 5431
BssSI C'ACGA_G
Cuts at: 152 3883 4591 152
Size: 3731 708 1173
Fragments arranged by size:
3731 1173 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2065 2989 2065
Size: 924 4688
BstBI TT'CG_AA
Cuts at: 2471 3973 2471
Size: 1502 4110
BstXI CCAn_nnnn'nTGG
Cuts at: 2604 2604
Size: 5612
BstYI r'GATC_y
Cuts at: 1 144 588 1955 2118 2637 3104 3127 3462
Size: 143 444 1367 163 519 467 23 335
Cuts at: 3462 3708 4014 4026 4112 5164 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1367 1052 519 467 449 444 335 306
246 163 143 86 23 12
BtgI C'CryG_G
Cuts at: 1793 1951 1793
Size: 158 5454
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3562 3743 3906 3562
Size: 181 163 5268
Fragments arranged by size:
5268 181 163
BtsI GCAGTG_nn'
Cuts at: 512 2855 512
Size: 2343 3269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1466 1501 1466
Size: 35 5577
DraI TTT'AAA
Cuts at: 2611 2611
Size: 5612
DrdI GACnn_nn'nnGTC
Cuts at: 3318 4662 3318
Size: 1344 4268
EaeI y'GGCC_r
Cuts at: 1055 1563 2250 2432 3197 3371 3762 3789 5134
Size: 508 687 182 765 174 391 27 1345
Cuts at: 5134 1055
Size: 1533
Fragments arranged by size:
1533 1345 765 687 508 391 182 174
27
EagI C'GGCC_G
Cuts at: 3197 3197
Size: 5612
EarI CTCTTCn'nnn_
Cuts at: 172 1007 3635 3845 172
Size: 835 2628 210 1939
Fragments arranged by size:
2628 1939 835 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3820 4562 4708 5422 3820
Size: 742 146 714 4010
Fragments arranged by size:
4010 742 714 146
EcoICRI GAG'CTC
Cuts at: 2038 2624 2038
Size: 586 5026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1114 1610 1691 1909 2160 2524 3437 3869
Size: 887 496 81 218 251 364 913 432
Cuts at: 3869 4222 4884 227
Size: 353 662 955
Fragments arranged by size:
955 913 887 662 496 432 364 353
251 218 81
EcoO109I rG'GnC_Cy
Cuts at: 162 348 1135 2751 162
Size: 186 787 1616 3023
Fragments arranged by size:
3023 1616 787 186
EcoRI G'AATT_C
Cuts at: 1258 2616 1258
Size: 1358 4254
EcoRV GAT'ATC
Cuts at: 441 441
Size: 5612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 217 1475 1767 1890 2290 3631 217
Size: 1258 292 123 400 1341 2198
Fragments arranged by size:
2198 1341 1258 400 292 123
FspI TGC'GCA
Cuts at: 3393 3393
Size: 5612
HaeII r_GCGC'y
Cuts at: 3046 3294 4524 5295 3046
Size: 248 1230 771 3363
Fragments arranged by size:
3363 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 310 342 1105 1137 1654 1686 3454
Size: 32 124 32 763 32 517 32 1768
Cuts at: 3454 3484 3486 3516 154
Size: 30 2 30 2250
Fragments arranged by size:
2250 1768 763 517 124 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2651 2651
Size: 5612
HindIII A'AGCT_T
Cuts at: 209 209
Size: 5612
KasI G'GCGC_C
Cuts at: 3042 3290 3042
Size: 248 5364
KpnI G_GTAC'C
Cuts at: 2632 2632
Size: 5612
MluI A'CGCG_T
Cuts at: 5045 5045
Size: 5612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4371 4555 4371
Size: 184 5428
MscI TGG'CCA
Cuts at: 1057 1565 2252 2434 3373 1057
Size: 508 687 182 939 3296
Fragments arranged by size:
3296 939 687 508 182
MslI CAynn'nnrTG
Cuts at: 505 1007 2199 2551 3601 3728 505
Size: 502 1192 352 1050 127 2389
Fragments arranged by size:
2389 1192 1050 502 352 127
MspA1I CmG'CkG
Cuts at: 735 1614 2723 3037 3397 4181 4426 735
Size: 879 1109 314 360 784 245 1921
Fragments arranged by size:
1921 1109 879 784 360 314 245
NaeI GCC'GGC
Cuts at: 3793 3793
Size: 5612
NarI GG'CG_CC
Cuts at: 3043 3291 3043
Size: 248 5364
NcoI C'CATG_G
Cuts at: 1793 1951 1793
Size: 158 5454
NdeI CA'TA_TG
Cuts at: 1788 1788
Size: 5612
NgoMIV G'CCGG_C
Cuts at: 3791 3791
Size: 5612
NsiI A_TGCA'T
Cuts at: 113 113
Size: 5612
NspI r_CATG'y
Cuts at: 343 778 1006 2665 3165 4768 343
Size: 435 228 1659 500 1603 1187
Fragments arranged by size:
1659 1603 1187 500 435 228
PciI A'CATG_T
Cuts at: 4764 4764
Size: 5612
PmeI GTTT'AAAC
Cuts at: 2611 2611
Size: 5612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 4058
Size: 32 5580
PpuMI rG'GwC_Cy
Cuts at: 162 162
Size: 5612
PspOMI G'GGCC_C
Cuts at: 1134 1134
Size: 5612
PspXI vC'TCGA_Gb
Cuts at: 799 799
Size: 5612
PstI C_TGCA'G
Cuts at: 2659 3344 2659
Size: 685 4927
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5612
PvuII CAG'CTG
Cuts at: 735 1614 3037 3397 735
Size: 879 1423 360 2950
Fragments arranged by size:
2950 1423 879 360
RsrII CG'GwC_CG
Cuts at: 3807 3807
Size: 5612
SacI G_AGCT'C
Cuts at: 2040 2626 2040
Size: 586 5026
SalI G'TCGA_C
Cuts at: 2649 2649
Size: 5612
SapI GCTCTTCn'nnn_
Cuts at: 3635 3845 3635
Size: 210 5402
SbfI CC_TGCA'GG
Cuts at: 2659 2659
Size: 5612
ScaI AGT'ACT
Cuts at: 5129 5129
Size: 5612
SexAI A'CCwGG_T
Cuts at: 1040 2126 1040
Size: 1086 4526
SfcI C'TryA_G
Cuts at: 33 1727 2655 3340 4308 4499 33
Size: 1694 928 685 968 191 1146
Fragments arranged by size:
1694 1146 968 928 685 191
SfoI GGC'GCC
Cuts at: 3044 3292 3044
Size: 248 5364
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5612
SmaI CCC'GGG
Cuts at: 2464 2634 2464
Size: 170 5442
SmlI C'TyrA_G
Cuts at: 799 2767 3682 4396 4658 799
Size: 1968 915 714 262 1753
Fragments arranged by size:
1968 1753 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5612
SphI G_CATG'C
Cuts at: 343 2665 3165 343
Size: 2322 500 2790
Fragments arranged by size:
2790 2322 500
SspI AAT'ATT
Cuts at: 1644 1644
Size: 5612
StyI C'CwwG_G
Cuts at: 581 633 1217 1566 1793 1951 2510 2746 3100
Size: 52 584 349 227 158 559 236 354
Cuts at: 3100 581
Size: 3093
Fragments arranged by size:
3093 584 559 354 349 236 227 158
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 337 3195 3328 3787 3996 337
Size: 2858 133 459 209 1953
Fragments arranged by size:
2858 1953 459 209 133
TatI w'GTAC_w
Cuts at: 2412 5127 2412
Size: 2715 2897
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5129 5161 5129
Size: 32 5580
Tth111I GACn'n_nGTC
Cuts at: 324 3409 324
Size: 3085 2527
XbaI T'CTAG_A
Cuts at: 2643 2643
Size: 5612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3107 3107
Size: 5612
XhoI C'TCGA_G
Cuts at: 799 799
Size: 5612
XmaI C'CCGG_G
Cuts at: 2462 2632 2462
Size: 170 5442
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BglI BmtI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PsiI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XmnI ZraI