Restriction map for
pF1KA1693
(Circular) (Six-base) MAPSORT of: pF1KA1693.seq Check: 819 from: 1 to: 5714
pF1KA1693 5714 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 564 833 1377 1628 2752 564
Size: 269 544 251 1124 3526
Fragments arranged by size:
3526 1124 544 269 251
Acc65I G'GTAC_C
Cuts at: 2730 2730
Size: 5714
AclI AA'CG_TT
Cuts at: 5554 5554
Size: 5714
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 1523 1781 2015 2021 2240 2522 3539 3971
Size: 813 258 234 6 219 282 1017 432
Cuts at: 3971 4718 4986 710
Size: 747 268 1438
Fragments arranged by size:
1438 1017 813 747 432 282 268 258
234 219 6
AflIII A'CryG_T
Cuts at: 2331 3066 4170 5147 2331
Size: 735 1104 977 2898
Fragments arranged by size:
2898 1104 977 735
AgeI A'CCGG_T
Cuts at: 4102 4102
Size: 5714
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1990 2215 1990
Size: 225 5489
AlwNI CAG_nnn'CTG
Cuts at: 889 3019 4586 889
Size: 2130 1567 2017
Fragments arranged by size:
2130 2017 1567
ApaLI G'TGCA_C
Cuts at: 4484 4484
Size: 5714
ApoI r'AATT_y
Cuts at: 2718 5067 5323 2718
Size: 2349 256 3109
Fragments arranged by size:
3109 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5714
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5714
AvaI C'yCGr_G
Cuts at: 2734 2734
Size: 5714
BamHI G'GATC_C
Cuts at: 2739 2739
Size: 5714
BanI G'GyrC_C
Cuts at: 2730 3144 3392 3427 2730
Size: 414 248 35 5017
Fragments arranged by size:
5017 414 248 35
BanII G_rGCy'C
Cuts at: 383 1196 2728 3758 383
Size: 813 1532 1030 2339
Fragments arranged by size:
2339 1532 1030 813
BbeI G_GCGC'C
Cuts at: 3148 3396 3148
Size: 248 5466
BbsI GAAGACnn'nnnn_
Cuts at: 812 1625 2168 812
Size: 813 543 4358
Fragments arranged by size:
4358 813 543
BbvCI CC'TCA_GC
Cuts at: 319 374 554 1132 1187 1367 319
Size: 55 180 578 55 180 4666
Fragments arranged by size:
4666 578 180 180 55 55
BciVI GTATCCnnnnn_n'
Cuts at: 3612 4373 5714 3612
Size: 761 1341 3612
Fragments arranged by size:
3612 1341 761
BclI T'GATC_A
Cuts at: 3234 3234
Size: 5714
BfrBI ATG'CAT
Cuts at: 2317 2317
Size: 5714
BglII A'GATC_T
Cuts at: 3229 5266 3229
Size: 2037 3677
BlpI GC'TnA_GC
Cuts at: 592 1405 1963 2188 2826 592
Size: 813 558 225 638 3480
Fragments arranged by size:
3480 813 638 558 225
Bme1580I G_kGCm'C
Cuts at: 3339 3432 4488 3339
Size: 93 1056 4565
Fragments arranged by size:
4565 1056 93
BmgBI CAC'GTC
Cuts at: 2127 2352 2127
Size: 225 5489
BmrI ACTGGGnnnn_n'
Cuts at: 423 877 1236 2926 3007 3342 5486 423
Size: 454 359 1690 81 335 2144 651
Fragments arranged by size:
2144 1690 651 454 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4081 4081
Size: 5714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 739 1285 1552 2224 472
Size: 267 546 267 672 3962
Fragments arranged by size:
3962 672 546 267 267
Bpu10I CC'TnA_GC
Cuts at: 319 374 382 554 1132 1187 1195 1367 319
Size: 55 8 172 578 55 8 172 4666
Fragments arranged by size:
4666 578 172 172 55 55 8 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1162 2890 3769 4261 4559 349
Size: 813 1728 879 492 298 1504
Fragments arranged by size:
1728 1504 879 813 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5418 5418
Size: 5714
BsaAI yAC'GTr
Cuts at: 2634 3067 2634
Size: 433 5281
BsaBI GATnn'nnATC
Cuts at: 6 3228 5265 6
Size: 3222 2037 455
Fragments arranged by size:
3222 2037 455
BsaHI Gr'CG_yC
Cuts at: 3145 3393 4095 5591 3145
Size: 248 702 1496 3268
Fragments arranged by size:
3268 1496 702 248
BsaWI w'CCGG_w
Cuts at: 504 1317 2903 3424 4102 4376 4523 4934 504
Size: 813 1586 521 678 274 147 411 1284
Fragments arranged by size:
1586 1284 813 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2089 2119 2089
Size: 30 5684
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 391 487 652 1171 1204 1300 1780 358
Size: 33 96 165 519 33 96 480 4292
Fragments arranged by size:
4292 519 480 165 96 96 33 33
BseYI C'CCAG_C
Cuts at: 185 998 3139 3897 4474 4970 185
Size: 813 2141 758 577 496 929
Fragments arranged by size:
2141 929 813 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1486 700
Size: 786 4928
BsiEI CG_ry'CG
Cuts at: 104 3256 3302 4510 104
Size: 3152 46 1208 1308
Fragments arranged by size:
3152 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 383 1196 2728 3506 3696 4488 383
Size: 813 1532 778 190 792 1609
Fragments arranged by size:
1609 1532 813 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 383 1196 2728 3339 3432 3506 3696 3758 4488
Size: 813 1532 611 93 74 190 62 730
Cuts at: 4488 383
Size: 1609
Fragments arranged by size:
1609 1532 813 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 504 1317 4934 504
Size: 813 3617 1284
Fragments arranged by size:
3617 1284 813
BspHI T'CATG_A
Cuts at: 849 4890 849
Size: 4041 1673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2764 2978 3280 3661 2764
Size: 214 302 381 4817
Fragments arranged by size:
4817 381 302 214
BsrBI CCG'CTC
Cuts at: 50 470 1283 4006 4060 5544 5709 50
Size: 420 813 2723 54 1484 165 55
Fragments arranged by size:
2723 1484 813 420 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3626 3626
Size: 5714
BsrFI r'CCGG_y
Cuts at: 121 934 3712 3893 4102 121
Size: 813 2778 181 209 1733
Fragments arranged by size:
2778 1733 813 209 181
BsrGI T'GTAC_A
Cuts at: 1677 1677
Size: 5714
BssSI C'ACGA_G
Cuts at: 3985 4343 3985
Size: 358 5356
BstAPI GCAn_nnn'nTGC
Cuts at: 3091 3091
Size: 5714
BstBI TT'CG_AA
Cuts at: 1555 4075 1555
Size: 2520 3194
BstXI CCAn_nnnn'nTGG
Cuts at: 2299 2299
Size: 5714
BstYI r'GATC_y
Cuts at: 1 2739 3206 3229 3564 3810 4822 4908 4920
Size: 2738 467 23 335 246 1012 86 12
Cuts at: 4920 5266 1
Size: 346 449
Fragments arranged by size:
2738 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 707 1520 707
Size: 813 4901
BtgI C'CryG_G
Cuts at: 107 750 1563 107
Size: 643 813 4258
Fragments arranged by size:
4258 813 643
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3664 3845 4008 3664
Size: 181 163 5370
Fragments arranged by size:
5370 181 163
BtsI GCAGTG_nn'
Cuts at: 2085 2957 2085
Size: 872 4842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 739 774 739
Size: 35 5679
DraI TTT'AAA
Cuts at: 2713 2713
Size: 5714
DraIII CAC_nnn'GTG
Cuts at: 2679 2679
Size: 5714
DrdI GACnn_nn'nnGTC
Cuts at: 3420 4278 3420
Size: 858 4856
EaeI y'GGCC_r
Cuts at: 260 1073 3299 3473 3864 3891 5236 260
Size: 813 2226 174 391 27 1345 738
Fragments arranged by size:
2226 1345 813 738 391 174 27
EagI C'GGCC_G
Cuts at: 3299 3299
Size: 5714
EarI CTCTTCn'nnn_
Cuts at: 284 906 1097 1709 1757 2147 2489 3737 3947
Size: 622 191 612 48 390 342 1248 210
Cuts at: 3947 284
Size: 2051
Fragments arranged by size:
2051 1248 622 612 390 342 210 191
48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3922 4232 4378 5524 3922
Size: 310 146 1146 4112
Fragments arranged by size:
4112 1146 310 146
EcoICRI GAG'CTC
Cuts at: 381 1194 2726 381
Size: 813 1532 3369
Fragments arranged by size:
3369 1532 813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 710 739 1285 1523 1552 1781 2015 2021
Size: 238 29 546 238 29 229 234 6
Cuts at: 2021 2224 2240 2522 3539 3971 4718 4986 472
Size: 203 16 282 1017 432 747 268 1200
Fragments arranged by size:
1200 1017 747 546 432 282 268 238
238 234 229 203 29 29 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 5714
EcoO109I rG'GnC_Cy
Cuts at: 492 534 1305 1347 1954 1990 2853 492
Size: 42 771 42 607 36 863 3353
Fragments arranged by size:
3353 863 771 607 42 42 36
EcoRI G'AATT_C
Cuts at: 2718 2718
Size: 5714
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3733 3733
Size: 5714
FspI TGC'GCA
Cuts at: 3495 3495
Size: 5714
HaeII r_GCGC'y
Cuts at: 3148 3396 4418 5397 3148
Size: 248 1022 979 3465
Fragments arranged by size:
3465 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 3556 3586 3588 3618 826
Size: 32 2698 30 2 30 2922
Fragments arranged by size:
2922 2698 32 30 30 2
HincII GTy'rAC
Cuts at: 816 1629 1899 2753 816
Size: 813 270 854 3777
Fragments arranged by size:
3777 854 813 270
HindIII A'AGCT_T
Cuts at: 349 1162 349
Size: 813 4901
KasI G'GCGC_C
Cuts at: 3144 3392 3144
Size: 248 5466
KpnI G_GTAC'C
Cuts at: 2734 2734
Size: 5714
MluI A'CGCG_T
Cuts at: 5147 5147
Size: 5714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 791 1604 4385 4569 791
Size: 813 2781 184 1936
Fragments arranged by size:
2781 1936 813 184
MscI TGG'CCA
Cuts at: 262 1075 3475 262
Size: 813 2400 2501
Fragments arranged by size:
2501 2400 813
MslI CAynn'nnrTG
Cuts at: 3703 3830 3703
Size: 127 5587
MspA1I CmG'CkG
Cuts at: 189 549 1002 1362 2825 3139 3499 4512 4757
Size: 360 453 360 1463 314 360 1013 245
Cuts at: 4757 189
Size: 1146
Fragments arranged by size:
1463 1146 1013 453 360 360 360 314
245
NaeI GCC'GGC
Cuts at: 123 936 3895 123
Size: 813 2959 1942
Fragments arranged by size:
2959 1942 813
NarI GG'CG_CC
Cuts at: 3145 3393 3145
Size: 248 5466
NcoI C'CATG_G
Cuts at: 107 1563 107
Size: 1456 4258
NgoMIV G'CCGG_C
Cuts at: 121 934 3893 121
Size: 813 2959 1942
Fragments arranged by size:
2959 1942 813
NsiI A_TGCA'T
Cuts at: 2319 2319
Size: 5714
NspI r_CATG'y
Cuts at: 2335 2767 3267 4174 2335
Size: 432 500 907 3875
Fragments arranged by size:
3875 907 500 432
PasI CC'CwG_GG
Cuts at: 525 671 1338 1484 525
Size: 146 667 146 4755
Fragments arranged by size:
4755 667 146 146
PciI A'CATG_T
Cuts at: 2331 4170 2331
Size: 1839 3875
PflMI CCAn_nnn'nTGG
Cuts at: 889 889
Size: 5714
PmeI GTTT'AAAC
Cuts at: 2713 2713
Size: 5714
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 319 351 1132 1164 4853 4885 319
Size: 32 781 32 3689 32 1148
Fragments arranged by size:
3689 1148 781 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 534 1347 1954 534
Size: 813 607 4294
Fragments arranged by size:
4294 813 607
PstI C_TGCA'G
Cuts at: 1754 2761 3446 1754
Size: 1007 685 4022
Fragments arranged by size:
4022 1007 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5714
PvuII CAG'CTG
Cuts at: 189 549 1002 1362 3139 3499 189
Size: 360 453 360 1777 360 2404
Fragments arranged by size:
2404 1777 453 360 360 360
RsrII CG'GwC_CG
Cuts at: 3909 3909
Size: 5714
SacI G_AGCT'C
Cuts at: 383 1196 2728 383
Size: 813 1532 3369
Fragments arranged by size:
3369 1532 813
SalI G'TCGA_C
Cuts at: 1627 2751 1627
Size: 1124 4590
SapI GCTCTTCn'nnn_
Cuts at: 2489 3737 3947 2489
Size: 1248 210 4256
Fragments arranged by size:
4256 1248 210
SbfI CC_TGCA'GG
Cuts at: 2761 2761
Size: 5714
ScaI AGT'ACT
Cuts at: 653 5231 653
Size: 4578 1136
SexAI A'CCwGG_T
Cuts at: 2675 2675
Size: 5714
SfcI C'TryA_G
Cuts at: 33 1750 2073 2580 2757 3442 4435 4626 33
Size: 1717 323 507 177 685 993 191 1121
Fragments arranged by size:
1717 1121 993 685 507 323 191 177
SfoI GGC'GCC
Cuts at: 3146 3394 3146
Size: 248 5466
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5714
SmaI CCC'GGG
Cuts at: 2736 2736
Size: 5714
SmlI C'TyrA_G
Cuts at: 364 1177 2869 3784 4276 4538 364
Size: 813 1692 915 492 262 1540
Fragments arranged by size:
1692 1540 915 813 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5714
SphI G_CATG'C
Cuts at: 2767 3267 2767
Size: 500 5214
StyI C'CwwG_G
Cuts at: 107 1563 2109 2166 2848 3202 107
Size: 1456 546 57 682 354 2619
Fragments arranged by size:
2619 1456 682 546 354 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3297 3430 3889 4098 3297
Size: 133 459 209 4913
Fragments arranged by size:
4913 459 209 133
TatI w'GTAC_w
Cuts at: 651 1677 2549 5229 651
Size: 1026 872 2680 1136
Fragments arranged by size:
2680 1136 1026 872
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5231 5263 5231
Size: 32 5682
Tth111I GACn'n_nGTC
Cuts at: 811 1624 3511 811
Size: 813 1887 3014
Fragments arranged by size:
3014 1887 813
XbaI T'CTAG_A
Cuts at: 159 972 2745 159
Size: 813 1773 3128
Fragments arranged by size:
3128 1773 813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3209 3209
Size: 5714
XmaI C'CCGG_G
Cuts at: 2734 2734
Size: 5714
XmnI GAAnn'nnTTC
Cuts at: 626 1439 626
Size: 813 4901
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BglI BmtI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI