Restriction map for
pF1KA1694
(Circular) (Six-base) MAPSORT of: pF1KA1694.seq Check: 9066 from: 1 to: 5432
pF1KA1694 5432 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 2408 2408
Size: 5432
AccI GT'mk_AC
Cuts at: 2470 2470
Size: 5432
Acc65I G'GTAC_C
Cuts at: 2448 2448
Size: 5432
AclI AA'CG_TT
Cuts at: 5272 5272
Size: 5432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 1615 2014 2392 3257 3689 4042 4704 1183
Size: 432 399 378 865 432 353 662 1911
Fragments arranged by size:
1911 865 662 432 432 399 378 353
AflIII A'CryG_T
Cuts at: 185 2784 4584 4865 185
Size: 2599 1800 281 752
Fragments arranged by size:
2599 1800 752 281
AhdI GACnn_n'nnGTC
Cuts at: 1248 1248
Size: 5432
AleI CACnn'nnGTG
Cuts at: 901 1945 901
Size: 1044 4388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 2173
Size: 32 5400
AlwNI CAG_nnn'CTG
Cuts at: 465 2056 2138 2253 2284 2737 4175 465
Size: 1591 82 115 31 453 1438 1722
Fragments arranged by size:
1722 1591 1438 453 115 82 31
ApaI G_GGCC'C
Cuts at: 1447 2021 1447
Size: 574 4858
ApaLI G'TGCA_C
Cuts at: 1747 4270 1747
Size: 2523 2909
ApoI r'AATT_y
Cuts at: 343 838 1783 2436 4785 5041 343
Size: 495 945 653 2349 256 734
Fragments arranged by size:
2349 945 734 653 495 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
AvaI C'yCGr_G
Cuts at: 126 1070 2452 126
Size: 944 1382 3106
Fragments arranged by size:
3106 1382 944
BamHI G'GATC_C
Cuts at: 2457 2457
Size: 5432
BanI G'GyrC_C
Cuts at: 214 305 2448 2862 3110 3145 214
Size: 91 2143 414 248 35 2501
Fragments arranged by size:
2501 2143 414 248 91 35
BanII G_rGCy'C
Cuts at: 237 998 1385 1447 1765 2021 2080 2305 2446
Size: 761 387 62 318 256 59 225 141
Cuts at: 2446 3476 237
Size: 1030 2193
Fragments arranged by size:
2193 1030 761 387 318 256 225 141
62 59
BbeI G_GCGC'C
Cuts at: 218 2866 3114 218
Size: 2648 248 2536
Fragments arranged by size:
2648 2536 248
BbsI GAAGACnn'nnnn_
Cuts at: 458 1628 458
Size: 1170 4262
BciVI GTATCCnnnnn_n'
Cuts at: 3330 4386 5432 3330
Size: 1056 1046 3330
Fragments arranged by size:
3330 1056 1046
BclI T'GATC_A
Cuts at: 2952 2952
Size: 5432
BglI GCCn_nnn'nGGC
Cuts at: 1348 2025 1348
Size: 677 4755
BglII A'GATC_T
Cuts at: 828 2367 2947 4984 828
Size: 1539 580 2037 1276
Fragments arranged by size:
2037 1539 1276 580
BlpI GC'TnA_GC
Cuts at: 1654 2074 2544 1654
Size: 420 470 4542
Fragments arranged by size:
4542 470 420
Bme1580I G_kGCm'C
Cuts at: 224 949 1058 1447 1751 2021 3057 3150 4274
Size: 725 109 389 304 270 1036 93 1124
Cuts at: 4274 224
Size: 1382
Fragments arranged by size:
1382 1124 1036 725 389 304 270 109
93
BmgBI CAC'GTC
Cuts at: 186 1947 186
Size: 1761 3671
BmrI ACTGGGnnnn_n'
Cuts at: 422 2644 2725 3060 5204 422
Size: 2222 81 335 2144 650
Fragments arranged by size:
2222 2144 650 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1538 1659 3799 1538
Size: 121 2140 3171
Fragments arranged by size:
3171 2140 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1287 1533 1954 2119 2230 1287
Size: 246 421 165 111 4489
Fragments arranged by size:
4489 421 246 165 111
Bpu10I CC'TnA_GC
Cuts at: 2299 2299
Size: 5432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1267 1471 2155 2608 3487 4201 4499 904
Size: 363 204 684 453 879 714 298 1837
Fragments arranged by size:
1837 879 714 684 453 363 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 154 548 1509 2260 5136 154
Size: 394 961 751 2876 450
Fragments arranged by size:
2876 961 751 450 394
BsaAI yAC'GTr
Cuts at: 1317 1476 1602 2785 1317
Size: 159 126 1183 3964
Fragments arranged by size:
3964 1183 159 126
BsaBI GATnn'nnATC
Cuts at: 6 2946 4983 6
Size: 2940 2037 455
Fragments arranged by size:
2940 2037 455
BsaHI Gr'CG_yC
Cuts at: 215 397 2405 2863 3111 3813 5309 215
Size: 182 2008 458 248 702 1496 338
Fragments arranged by size:
2008 1496 702 458 338 248 182
BsaWI w'CCGG_w
Cuts at: 653 1172 1199 2621 3142 3820 4231 4378 4652
Size: 519 27 1422 521 678 411 147 274
Cuts at: 4652 653
Size: 1433
Fragments arranged by size:
1433 1422 678 521 519 411 274 147
27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 1852 1882 418
Size: 30 1404 30 3968
Fragments arranged by size:
3968 1404 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 172
Size: 5432
BseYI C'CCAG_C
Cuts at: 174 356 629 1234 1440 1812 2857 3615 4280
Size: 182 273 605 206 372 1045 758 665
Cuts at: 4280 4688 174
Size: 408 918
Fragments arranged by size:
1045 918 758 665 605 408 372 273
206 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1213 928
Size: 285 5147
BsiEI CG_ry'CG
Cuts at: 104 1200 1458 2974 3020 4250 104
Size: 1096 258 1516 46 1230 1286
Fragments arranged by size:
1516 1286 1230 1096 258 46
BsiHKAI G_wGCw'C
Cuts at: 998 1751 1942 2080 2150 2305 2446 3224 3414
Size: 753 191 138 70 155 141 778 190
Cuts at: 3414 4274 998
Size: 860 2156
Fragments arranged by size:
2156 860 778 753 191 190 155 141
138 70
BsmI GAATG_Cn'
Cuts at: 483 483
Size: 5432
BsmBI CGTCTCn'nnnn_
Cuts at: 732 1526 2198 732
Size: 794 672 3966
Fragments arranged by size:
3966 794 672
Bsp1286I G_dGCh'C
Cuts at: 224 237 949 998 1058 1385 1447 1751 1765
Size: 13 712 49 60 327 62 304 14
Cuts at: 1765 1942 2021 2080 2150 2305 2446 3057 3150
Size: 177 79 59 70 155 141 611 93
Cuts at: 3150 3224 3414 3476 4274 224
Size: 74 190 62 798 1382
Fragments arranged by size:
1382 798 712 611 327 304 190 177
155 141 93 79 74 70 62 62
60 59 49 14 13
BspEI T'CCGG_A
Cuts at: 653 1172 653
Size: 519 4913
BspHI T'CATG_A
Cuts at: 3864 3864
Size: 5432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 557 2482 2696 2998 3379 557
Size: 1925 214 302 381 2610
Fragments arranged by size:
2610 1925 381 302 214
BsrBI CCG'CTC
Cuts at: 50 864 3724 3778 5262 5427 50
Size: 814 2860 54 1484 165 55
Fragments arranged by size:
2860 1484 814 165 55 54
BsrDI GCAATG_nn'
Cuts at: 583 3344 583
Size: 2761 2671
BsrFI r'CCGG_y
Cuts at: 1347 1666 1979 3430 3611 1347
Size: 319 313 1451 181 3168
Fragments arranged by size:
3168 1451 319 313 181
BssSI C'ACGA_G
Cuts at: 3703 4411 3703
Size: 708 4724
BstAPI GCAn_nnn'nTGC
Cuts at: 854 2809 854
Size: 1955 3477
BstBI TT'CG_AA
Cuts at: 3793 3793
Size: 5432
BstXI CCAn_nnnn'nTGG
Cuts at: 629 1089 629
Size: 460 4972
BstYI r'GATC_y
Cuts at: 1 329 650 828 1310 1862 2367 2457 2924
Size: 328 321 178 482 552 505 90 467
Cuts at: 2924 2947 3282 3528 3834 3846 3932 4984 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 552 505 482 467 449 335 328
321 306 246 178 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 349 349
Size: 5432
BtgI C'CryG_G
Cuts at: 107 310 875 902 1853 107
Size: 203 565 27 951 3686
Fragments arranged by size:
3686 951 565 203 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3382 3563 3726 3382
Size: 181 163 5088
Fragments arranged by size:
5088 181 163
BtsI GCAGTG_nn'
Cuts at: 852 1056 1337 2032 2675 852
Size: 204 281 695 643 3609
Fragments arranged by size:
3609 695 643 281 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2181 2216 2181
Size: 35 5397
DraI TTT'AAA
Cuts at: 2431 2431
Size: 5432
DrdI GACnn_nn'nnGTC
Cuts at: 289 3138 4482 289
Size: 2849 1344 1239
Fragments arranged by size:
2849 1344 1239
EaeI y'GGCC_r
Cuts at: 380 1091 1358 1526 3017 3191 3582 3609 4954
Size: 711 267 168 1491 174 391 27 1345
Cuts at: 4954 380
Size: 858
Fragments arranged by size:
1491 1345 858 711 391 267 174 168
27
EagI C'GGCC_G
Cuts at: 3017 3017
Size: 5432
EarI CTCTTCn'nnn_
Cuts at: 242 1121 2305 3455 3665 242
Size: 879 1184 1150 210 2009
Fragments arranged by size:
2009 1184 1150 879 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 390 3640 4382 4528 5242 390
Size: 3250 742 146 714 580
Fragments arranged by size:
3250 742 714 580 146
EcoICRI GAG'CTC
Cuts at: 996 2078 2303 2444 996
Size: 1082 225 141 3984
Fragments arranged by size:
3984 1082 225 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 1287 1533 1615 1954 2014 2119 2230 2392
Size: 104 246 82 339 60 105 111 162
Cuts at: 2392 3257 3689 4042 4704 1183
Size: 865 432 353 662 1911
Fragments arranged by size:
1911 865 662 432 353 339 246 162
111 105 104 82 60
EcoO109I rG'GnC_Cy
Cuts at: 711 1074 1444 1816 2571 711
Size: 363 370 372 755 3572
Fragments arranged by size:
3572 755 372 370 363
EcoRI G'AATT_C
Cuts at: 838 2436 838
Size: 1598 3834
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5432
FspI TGC'GCA
Cuts at: 3213 3213
Size: 5432
HaeII r_GCGC'y
Cuts at: 218 357 1354 2866 3114 4344 5115 218
Size: 139 997 1512 248 1230 771 535
Fragments arranged by size:
1512 1230 997 771 535 248 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1234 1266 3274 3304 3306 3336 1234
Size: 32 2008 30 2 30 3330
Fragments arranged by size:
3330 2008 32 30 30 2
HincII GTy'rAC
Cuts at: 2471 2471
Size: 5432
HindIII A'AGCT_T
Cuts at: 2380 2380
Size: 5432
KasI G'GCGC_C
Cuts at: 214 2862 3110 214
Size: 2648 248 2536
Fragments arranged by size:
2648 2536 248
KpnI G_GTAC'C
Cuts at: 2452 2452
Size: 5432
MfeI C'AATT_G
Cuts at: 446 446
Size: 5432
MluI A'CGCG_T
Cuts at: 4865 4865
Size: 5432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2207 4191 4375 2207
Size: 1984 184 3264
Fragments arranged by size:
3264 1984 184
MscI TGG'CCA
Cuts at: 1528 3193 1528
Size: 1665 3767
MslI CAynn'nnrTG
Cuts at: 112 208 784 889 901 1756 1945 3421 3548
Size: 96 576 105 12 855 189 1476 127
Cuts at: 3548 112
Size: 1996
Fragments arranged by size:
1996 1476 855 576 189 127 105 96
12
MspA1I CmG'CkG
Cuts at: 171 312 462 629 1971 1986 2178 2353 2543
Size: 141 150 167 1342 15 192 175 190
Cuts at: 2543 2857 3217 4001 4246 171
Size: 314 360 784 245 1357
Fragments arranged by size:
1357 1342 784 360 314 245 192 190
175 167 150 141 15
NaeI GCC'GGC
Cuts at: 1349 1668 3613 1349
Size: 319 1945 3168
Fragments arranged by size:
3168 1945 319
NarI GG'CG_CC
Cuts at: 215 2863 3111 215
Size: 2648 248 2536
Fragments arranged by size:
2648 2536 248
NcoI C'CATG_G
Cuts at: 107 875 1853 107
Size: 768 978 3686
Fragments arranged by size:
3686 978 768
NgoMIV G'CCGG_C
Cuts at: 1347 1666 3611 1347
Size: 319 1945 3168
Fragments arranged by size:
3168 1945 319
NspI r_CATG'y
Cuts at: 1041 1827 1962 2485 2985 4588 1041
Size: 786 135 523 500 1603 1885
Fragments arranged by size:
1885 1603 786 523 500 135
PciI A'CATG_T
Cuts at: 4584 4584
Size: 5432
PflMI CCAn_nnn'nTGG
Cuts at: 1854 1854
Size: 5432
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5432
PmlI CAC'GTG
Cuts at: 1317 1317
Size: 5432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 5400
PpuMI rG'GwC_Cy
Cuts at: 1074 1816 1074
Size: 742 4690
PshAI GACnn'nnGTC
Cuts at: 2272 2272
Size: 5432
PspOMI G'GGCC_C
Cuts at: 1443 2017 1443
Size: 574 4858
PstI C_TGCA'G
Cuts at: 275 533 692 859 1423 1919 2323 2479 3164
Size: 258 159 167 564 496 404 156 685
Cuts at: 3164 275
Size: 2543
Fragments arranged by size:
2543 685 564 496 404 258 167 159
156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5432
PvuII CAG'CTG
Cuts at: 462 1971 2178 2353 2857 3217 462
Size: 1509 207 175 504 360 2677
Fragments arranged by size:
2677 1509 504 360 207 175
RsrII CG'GwC_CG
Cuts at: 3627 3627
Size: 5432
SacI G_AGCT'C
Cuts at: 998 2080 2305 2446 998
Size: 1082 225 141 3984
Fragments arranged by size:
3984 1082 225 141
SacII CC_GC'GG
Cuts at: 313 313
Size: 5432
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5432
SanDI GG'GwC_CC
Cuts at: 1816 1816
Size: 5432
SapI GCTCTTCn'nnn_
Cuts at: 242 3455 3665 242
Size: 3213 210 2009
Fragments arranged by size:
3213 2009 210
SbfI CC_TGCA'GG
Cuts at: 692 2479 692
Size: 1787 3645
ScaI AGT'ACT
Cuts at: 490 4949 490
Size: 4459 973
SexAI A'CCwGG_T
Cuts at: 1085 1244 1085
Size: 159 5273
SfcI C'TryA_G
Cuts at: 33 271 438 529 688 855 1419 1692 1915
Size: 238 167 91 159 167 564 273 223
Cuts at: 1915 2319 2475 3160 4128 4319 33
Size: 404 156 685 968 191 1146
Fragments arranged by size:
1146 968 685 564 404 273 238 223
191 167 167 159 156 91
SfoI GGC'GCC
Cuts at: 216 2864 3112 216
Size: 2648 248 2536
Fragments arranged by size:
2648 2536 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5432
SmaI CCC'GGG
Cuts at: 1072 2454 1072
Size: 1382 4050
SmlI C'TyrA_G
Cuts at: 919 1282 1486 2170 2587 3502 4216 4478 919
Size: 363 204 684 417 915 714 262 1873
Fragments arranged by size:
1873 915 714 684 417 363 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5432
SphI G_CATG'C
Cuts at: 1041 1827 2485 2985 1041
Size: 786 658 500 3488
Fragments arranged by size:
3488 786 658 500
StyI C'CwwG_G
Cuts at: 107 875 1586 1853 2566 2920 107
Size: 768 711 267 713 354 2619
Fragments arranged by size:
2619 768 713 711 354 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 778 833 1924 3015 3148 3607 3816 778
Size: 55 1091 1091 133 459 209 2394
Fragments arranged by size:
2394 1091 1091 459 209 133 55
TatI w'GTAC_w
Cuts at: 488 974 4947 488
Size: 486 3973 973
Fragments arranged by size:
3973 973 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1852 1884 4949 4981 1852
Size: 32 3065 32 2303
Fragments arranged by size:
3065 2303 32 32
Tth111I GACn'n_nGTC
Cuts at: 3229 3229
Size: 5432
XbaI T'CTAG_A
Cuts at: 2463 2463
Size: 5432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 1242 2927 171
Size: 1071 1685 2676
Fragments arranged by size:
2676 1685 1071
XmaI C'CCGG_G
Cuts at: 1070 2452 1070
Size: 1382 4050
XmnI GAAnn'nnTTC
Cuts at: 455 1024 455
Size: 569 4863
ZraI GAC'GTC
Cuts at: 2406 2406
Size: 5432
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI