Restriction map for
pF1KA1700
(Circular) (Six-base) MAPSORT of: pF1KA1700.seq Check: 6469 from: 1 to: 5108
pF1KA1700 5108 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 463 473 463
Size: 10 5098
AccI GT'mk_AC
Cuts at: 2146 2146
Size: 5108
Acc65I G'GTAC_C
Cuts at: 2124 2124
Size: 5108
AclI AA'CG_TT
Cuts at: 4948 4948
Size: 5108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1520 2933 3365 3718 4380 389
Size: 1131 1413 432 353 662 1117
Fragments arranged by size:
1413 1131 1117 662 432 353
AflII C'TTAA_G
Cuts at: 1714 1714
Size: 5108
AflIII A'CryG_T
Cuts at: 899 2460 4260 4541 899
Size: 1561 1800 281 1466
Fragments arranged by size:
1800 1561 1466 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1573 1882 1573
Size: 309 4799
AlwNI CAG_nnn'CTG
Cuts at: 1775 1988 2413 3851 1775
Size: 213 425 1438 3032
Fragments arranged by size:
3032 1438 425 213
ApaLI G'TGCA_C
Cuts at: 832 3946 832
Size: 3114 1994
ApoI r'AATT_y
Cuts at: 590 761 1999 2112 4461 4717 590
Size: 171 1238 113 2349 256 981
Fragments arranged by size:
2349 1238 981 256 171 113
AseI AT'TA_AT
Cuts at: 20 245 20
Size: 225 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
AvaI C'yCGr_G
Cuts at: 712 2128 712
Size: 1416 3692
BamHI G'GATC_C
Cuts at: 2133 2133
Size: 5108
BanI G'GyrC_C
Cuts at: 1585 2124 2538 2786 2821 1585
Size: 539 414 248 35 3872
Fragments arranged by size:
3872 539 414 248 35
BanII G_rGCy'C
Cuts at: 311 1960 2122 3152 311
Size: 1649 162 1030 2267
Fragments arranged by size:
2267 1649 1030 162
BbeI G_GCGC'C
Cuts at: 2542 2790 2542
Size: 248 4860
BbsI GAAGACnn'nnnn_
Cuts at: 1286 1286
Size: 5108
BbvCI CC'TCA_GC
Cuts at: 1815 1815
Size: 5108
BciVI GTATCCnnnnn_n'
Cuts at: 3006 4062 5108 3006
Size: 1056 1046 3006
Fragments arranged by size:
3006 1056 1046
BclI T'GATC_A
Cuts at: 2628 2628
Size: 5108
BglI GCCn_nnn'nGGC
Cuts at: 1345 1718 1345
Size: 373 4735
BglII A'GATC_T
Cuts at: 2623 4660 2623
Size: 2037 3071
BlpI GC'TnA_GC
Cuts at: 2220 2220
Size: 5108
Bme1580I G_kGCm'C
Cuts at: 836 1190 1199 2733 2826 3950 836
Size: 354 9 1534 93 1124 1994
Fragments arranged by size:
1994 1534 1124 354 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 2320 2401 2736 4880 2320
Size: 81 335 2144 2548
Fragments arranged by size:
2548 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 646 3475 646
Size: 2829 2279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1034 1075 460
Size: 574 41 4493
Fragments arranged by size:
4493 574 41
Bpu10I CC'TnA_GC
Cuts at: 1815 1815
Size: 5108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1279 2284 3163 3877 4175 1024
Size: 255 1005 879 714 298 1957
Fragments arranged by size:
1957 1005 879 714 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 1874 2103 4812 1874
Size: 229 2709 2170
Fragments arranged by size:
2709 2170 229
BsaAI yAC'GTr
Cuts at: 2461 2461
Size: 5108
BsaBI GATnn'nnATC
Cuts at: 6 2622 4659 6
Size: 2616 2037 455
Fragments arranged by size:
2616 2037 455
BsaHI Gr'CG_yC
Cuts at: 1111 2539 2787 3489 4985 1111
Size: 1428 248 702 1496 1234
Fragments arranged by size:
1496 1428 1234 702 248
BsaWI w'CCGG_w
Cuts at: 2297 2818 3496 3907 4054 4328 2297
Size: 521 678 411 147 274 3077
Fragments arranged by size:
3077 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 936 966 1871 1901 936
Size: 30 905 30 4143
Fragments arranged by size:
4143 905 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1359 1359
Size: 5108
BseYI C'CCAG_C
Cuts at: 844 1189 1198 2043 2533 3291 3956 4364 844
Size: 345 9 845 490 758 665 408 1588
Fragments arranged by size:
1588 845 758 665 490 408 345 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1225 1240 1033
Size: 192 15 4901
Fragments arranged by size:
4901 192 15
BsiEI CG_ry'CG
Cuts at: 104 2650 2696 3926 104
Size: 2546 46 1230 1286
Fragments arranged by size:
2546 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 311 836 2122 2900 3090 3950 311
Size: 525 1286 778 190 860 1469
Fragments arranged by size:
1469 1286 860 778 525 190
BsmI GAATG_Cn'
Cuts at: 318 1560 318
Size: 1242 3866
BsmBI CGTCTCn'nnnn_
Cuts at: 1102 1102
Size: 5108
Bsp1286I G_dGCh'C
Cuts at: 311 836 1190 1199 1960 2122 2733 2826 2900
Size: 525 354 9 761 162 611 93 74
Cuts at: 2900 3090 3152 3950 311
Size: 190 62 798 1469
Fragments arranged by size:
1469 798 761 611 525 354 190 162
93 74 62 9
BspHI T'CATG_A
Cuts at: 884 3540 884
Size: 2656 2452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 463 473 550 2158 2372 2674 3055 463
Size: 10 77 1608 214 302 381 2516
Fragments arranged by size:
2516 1608 381 302 214 77 10
BsrBI CCG'CTC
Cuts at: 50 857 3400 3454 4938 5103 50
Size: 807 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 807 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3020 3020
Size: 5108
BsrFI r'CCGG_y
Cuts at: 200 3106 3287 200
Size: 2906 181 2021
Fragments arranged by size:
2906 2021 181
BssSI C'ACGA_G
Cuts at: 3379 4087 3379
Size: 708 4400
BstAPI GCAn_nnn'nTGC
Cuts at: 1988 2485 1988
Size: 497 4611
BstBI TT'CG_AA
Cuts at: 3469 3469
Size: 5108
BstXI CCAn_nnnn'nTGG
Cuts at: 1413 1413
Size: 5108
BstYI r'GATC_y
Cuts at: 1 848 2133 2600 2623 2958 3204 3510 3522
Size: 847 1285 467 23 335 246 306 12
Cuts at: 3522 3608 4660 1
Size: 86 1052 449
Fragments arranged by size:
1285 1052 847 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 769 107
Size: 662 4446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 852 3058 3239 3402 852
Size: 2206 181 163 2558
Fragments arranged by size:
2558 2206 181 163
BtsI GCAGTG_nn'
Cuts at: 1587 1612 1839 2351 1587
Size: 25 227 512 4344
Fragments arranged by size:
4344 512 227 25
DraI TTT'AAA
Cuts at: 2107 2107
Size: 5108
DraIII CAC_nnn'GTG
Cuts at: 1129 1129
Size: 5108
DrdI GACnn_nn'nnGTC
Cuts at: 2814 4158 2814
Size: 1344 3764
EaeI y'GGCC_r
Cuts at: 202 2693 2867 3258 3285 4630 202
Size: 2491 174 391 27 1345 680
Fragments arranged by size:
2491 1345 680 391 174 27
EagI C'GGCC_G
Cuts at: 2693 2693
Size: 5108
EarI CTCTTCn'nnn_
Cuts at: 408 439 883 1946 2033 3131 3341 408
Size: 31 444 1063 87 1098 210 2175
Fragments arranged by size:
2175 1098 1063 444 210 87 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 849 1953 3316 4058 4204 4918 849
Size: 1104 1363 742 146 714 1039
Fragments arranged by size:
1363 1104 1039 742 714 146
EcoICRI GAG'CTC
Cuts at: 309 2120 309
Size: 1811 3297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 460 1034 1075 1520 2933 3365 3718 4380
Size: 71 574 41 445 1413 432 353 662
Cuts at: 4380 389
Size: 1117
Fragments arranged by size:
1413 1117 662 574 445 432 353 71
41
EcoNI CCTnn'n_nnAGG
Cuts at: 634 634
Size: 5108
EcoO109I rG'GnC_Cy
Cuts at: 1598 2247 1598
Size: 649 4459
EcoRI G'AATT_C
Cuts at: 590 2112 590
Size: 1522 3586
EcoRV GAT'ATC
Cuts at: 1317 1734 1317
Size: 417 4691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 720 1320 3127 720
Size: 600 1807 2701
Fragments arranged by size:
2701 1807 600
FspI TGC'GCA
Cuts at: 1900 2889 1900
Size: 989 4119
HaeII r_GCGC'y
Cuts at: 1246 2542 2790 4020 4791 1246
Size: 1296 248 1230 771 1563
Fragments arranged by size:
1563 1296 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 114 146 364 379 396 411 1306 1338 1871
Size: 32 218 15 17 15 895 32 533
Cuts at: 1871 1903 1997 2014 2029 2046 2950 2980 2982
Size: 32 94 17 15 17 904 30 2
Cuts at: 2982 3012 114
Size: 30 2210
Fragments arranged by size:
2210 904 895 533 218 94 32 32
32 30 30 17 17 17 15 15
15 2
HincII GTy'rAC
Cuts at: 339 2147 339
Size: 1808 3300
HindIII A'AGCT_T
Cuts at: 262 914 262
Size: 652 4456
KasI G'GCGC_C
Cuts at: 2538 2786 2538
Size: 248 4860
KpnI G_GTAC'C
Cuts at: 2128 2128
Size: 5108
MluI A'CGCG_T
Cuts at: 4541 4541
Size: 5108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 755 3867 4051 755
Size: 3112 184 1812
Fragments arranged by size:
3112 1812 184
MscI TGG'CCA
Cuts at: 2869 2869
Size: 5108
MslI CAynn'nnrTG
Cuts at: 1622 3097 3224 1622
Size: 1475 127 3506
Fragments arranged by size:
3506 1475 127
MspA1I CmG'CkG
Cuts at: 1233 1775 1856 1866 2219 2533 2893 3677 3922
Size: 542 81 10 353 314 360 784 245
Cuts at: 3922 1233
Size: 2419
Fragments arranged by size:
2419 784 542 360 353 314 245 81
10
NaeI GCC'GGC
Cuts at: 202 3289 202
Size: 3087 2021
NarI GG'CG_CC
Cuts at: 2539 2787 2539
Size: 248 4860
NcoI C'CATG_G
Cuts at: 107 107
Size: 5108
NgoMIV G'CCGG_C
Cuts at: 200 3287 200
Size: 3087 2021
NspI r_CATG'y
Cuts at: 903 2161 2661 4264 903
Size: 1258 500 1603 1747
Fragments arranged by size:
1747 1603 1258 500
PciI A'CATG_T
Cuts at: 899 4260 899
Size: 3361 1747
PflMI CCAn_nnn'nTGG
Cuts at: 234 981 1775 234
Size: 747 794 3567
Fragments arranged by size:
3567 794 747
PfoI T'CCnGG_A
Cuts at: 980 980
Size: 5108
PmeI GTTT'AAAC
Cuts at: 2107 2107
Size: 5108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 5076
PpuMI rG'GwC_Cy
Cuts at: 1598 1598
Size: 5108
PstI C_TGCA'G
Cuts at: 1523 1861 2155 2840 1523
Size: 338 294 685 3791
Fragments arranged by size:
3791 685 338 294
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5108
PvuII CAG'CTG
Cuts at: 1775 1856 1866 2533 2893 1775
Size: 81 10 667 360 3990
Fragments arranged by size:
3990 667 360 81 10
RsrII CG'GwC_CG
Cuts at: 3303 3303
Size: 5108
SacI G_AGCT'C
Cuts at: 311 2122 311
Size: 1811 3297
SalI G'TCGA_C
Cuts at: 2145 2145
Size: 5108
SapI GCTCTTCn'nnn_
Cuts at: 439 2033 3131 3341 439
Size: 1594 1098 210 2206
Fragments arranged by size:
2206 1594 1098 210
SbfI CC_TGCA'GG
Cuts at: 2155 2155
Size: 5108
ScaI AGT'ACT
Cuts at: 4625 4625
Size: 5108
SfcI C'TryA_G
Cuts at: 33 1519 1851 1857 2151 2836 3804 3995 33
Size: 1486 332 6 294 685 968 191 1146
Fragments arranged by size:
1486 1146 968 685 332 294 191 6
SfoI GGC'GCC
Cuts at: 2540 2788 2540
Size: 248 4860
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5108
SmaI CCC'GGG
Cuts at: 2130 2130
Size: 5108
SmlI C'TyrA_G
Cuts at: 1039 1294 1714 2263 3178 3892 4154 1039
Size: 255 420 549 915 714 262 1993
Fragments arranged by size:
1993 915 714 549 420 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5108
SphI G_CATG'C
Cuts at: 2161 2661 2161
Size: 500 4608
StuI AGG'CCT
Cuts at: 1533 1533
Size: 5108
StyI C'CwwG_G
Cuts at: 107 2242 2596 107
Size: 2135 354 2619
Fragments arranged by size:
2619 2135 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1554 2691 2824 3283 3492 1554
Size: 1137 133 459 209 3170
Fragments arranged by size:
3170 1137 459 209 133
TatI w'GTAC_w
Cuts at: 423 4623 423
Size: 4200 908
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4625 4657 4625
Size: 32 5076
Tth111I GACn'n_nGTC
Cuts at: 1885 2905 1885
Size: 1020 4088
XbaI T'CTAG_A
Cuts at: 2139 2139
Size: 5108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 121 1999 2603 121
Size: 1878 604 2626
Fragments arranged by size:
2626 1878 604
XmaI C'CCGG_G
Cuts at: 2128 2128
Size: 5108
XmnI GAAnn'nnTTC
Cuts at: 448 448
Size: 5108
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI