Restriction map for
pF1KA1708
(Circular) (Six-base) MAPSORT of: pF1KA1708.seq Check: 8122 from: 1 to: 8096
pF1KA1708 8096 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1501 2649 1501
Size: 1148 6948
AccI GT'mk_AC
Cuts at: 3270 4519 5134 3270
Size: 1249 615 6232
Fragments arranged by size:
6232 1249 615
Acc65I G'GTAC_C
Cuts at: 5112 5112
Size: 8096
AclI AA'CG_TT
Cuts at: 1180 7936 1180
Size: 6756 1340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1142 1279 2570 3211 3464 3569 3848 4063
Size: 172 137 1291 641 253 105 279 215
Cuts at: 4063 4118 4334 4780 5921 6353 6706 7368 970
Size: 55 216 446 1141 432 353 662 1698
Fragments arranged by size:
1698 1291 1141 662 641 446 432 353
279 253 216 215 172 137 105 55
AflIII A'CryG_T
Cuts at: 682 1065 1367 5448 7248 7529 682
Size: 383 302 4081 1800 281 1249
Fragments arranged by size:
4081 1800 1249 383 302 281
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 8096
AleI CACnn'nnGTG
Cuts at: 4080 4080
Size: 8096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1283 1283
Size: 8096
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2610 2642 2610
Size: 32 8064
AlwNI CAG_nnn'CTG
Cuts at: 940 1810 1972 2088 2671 3856 4874 5401 6839
Size: 870 162 116 583 1185 1018 527 1438
Cuts at: 6839 940
Size: 2197
Fragments arranged by size:
2197 1438 1185 1018 870 583 527 162
116
ApaLI G'TGCA_C
Cuts at: 6934 6934
Size: 8096
ApoI r'AATT_y
Cuts at: 635 659 892 2132 2717 3770 5015 5100 7449
Size: 24 233 1240 585 1053 1245 85 2349
Cuts at: 7449 7705 635
Size: 256 1026
Fragments arranged by size:
2349 1245 1240 1053 1026 585 256 233
85 24
AseI AT'TA_AT
Cuts at: 20 3155 20
Size: 3135 4961
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8096
AvaI C'yCGr_G
Cuts at: 452 1114 5116 452
Size: 662 4002 3432
Fragments arranged by size:
4002 3432 662
AvrII C'CTAG_G
Cuts at: 234 4542 234
Size: 4308 3788
BamHI G'GATC_C
Cuts at: 5121 5121
Size: 8096
BanI G'GyrC_C
Cuts at: 115 4456 4845 5112 5526 5774 5809 115
Size: 4341 389 267 414 248 35 2402
Fragments arranged by size:
4341 2402 414 389 267 248 35
BanII G_rGCy'C
Cuts at: 133 560 778 1307 2703 3701 4653 5110 6140
Size: 427 218 529 1396 998 952 457 1030
Cuts at: 6140 133
Size: 2089
Fragments arranged by size:
2089 1396 1030 998 952 529 457 427
218
BbeI G_GCGC'C
Cuts at: 119 5530 5778 119
Size: 5411 248 2437
Fragments arranged by size:
5411 2437 248
BbsI GAAGACnn'nnnn_
Cuts at: 222 3217 3470 4266 4340 222
Size: 2995 253 796 74 3978
Fragments arranged by size:
3978 2995 796 253 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2665 2699 2665
Size: 34 8062
BciVI GTATCCnnnnn_n'
Cuts at: 337 2133 4902 5994 7050 8096 337
Size: 1796 2769 1092 1056 1046 337
Fragments arranged by size:
2769 1796 1092 1056 1046 337
BclI T'GATC_A
Cuts at: 1482 1893 5616 1482
Size: 411 3723 3962
Fragments arranged by size:
3962 3723 411
BfrBI ATG'CAT
Cuts at: 1450 2686 1450
Size: 1236 6860
BglII A'GATC_T
Cuts at: 296 1569 3387 3483 4590 5048 5064 5611 7648
Size: 1273 1818 96 1107 458 16 547 2037
Cuts at: 7648 296
Size: 744
Fragments arranged by size:
2037 1818 1273 1107 744 547 458 96
16
BlpI GC'TnA_GC
Cuts at: 5208 5208
Size: 8096
Bme1580I G_kGCm'C
Cuts at: 5721 5814 6938 5721
Size: 93 1124 6879
Fragments arranged by size:
6879 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 448 4205 4225 4852 5308 5389 5724 7868 448
Size: 3757 20 627 456 81 335 2144 676
Fragments arranged by size:
3757 2144 676 627 456 335 81 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6463 6463
Size: 8096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 509 674 980 4005 4436 398
Size: 111 165 306 3025 431 4058
Fragments arranged by size:
4058 3025 431 306 165 111
Bpu10I CC'TnA_GC
Cuts at: 2645 3353 2645
Size: 708 7388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2866 2905 5272 6151 6865 7163 2866
Size: 39 2367 879 714 298 3799
Fragments arranged by size:
3799 2367 879 714 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 2150 7800 2150
Size: 5650 2446
BsaAI yAC'GTr
Cuts at: 685 5449 685
Size: 4764 3332
BsaBI GATnn'nnATC
Cuts at: 6 5610 7647 6
Size: 5604 2037 455
Fragments arranged by size:
5604 2037 455
BsaHI Gr'CG_yC
Cuts at: 116 5527 5775 6477 7973 116
Size: 5411 248 702 1496 239
Fragments arranged by size:
5411 1496 702 248 239
BsaWI w'CCGG_w
Cuts at: 5285 5806 6484 6895 7042 7316 5285
Size: 521 678 411 147 274 6065
Fragments arranged by size:
6065 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 265 295 1662 1692 4003 4033 115
Size: 30 120 30 1367 30 2311 30 4178
Fragments arranged by size:
4178 2311 1367 120 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1540 1927 1930 1933 1936 1939 2908 3984 4135
Size: 387 3 3 3 3 969 1076 151
Cuts at: 4135 1540
Size: 5501
Fragments arranged by size:
5501 1076 969 387 151 3 3 3
3
BseYI C'CCAG_C
Cuts at: 284 2602 2702 5521 6279 6944 7352 284
Size: 2318 100 2819 758 665 408 1028
Fragments arranged by size:
2819 2318 1028 758 665 408 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2684 3147 2684
Size: 463 7633
BsiEI CG_ry'CG
Cuts at: 104 5638 5684 6914 104
Size: 5534 46 1230 1286
Fragments arranged by size:
5534 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 133 560 778 1307 3701 4121 4653 5110 5888
Size: 427 218 529 2394 420 532 457 778
Cuts at: 5888 6078 6938 133
Size: 190 860 1291
Fragments arranged by size:
2394 1291 860 778 532 529 457 427
420 218 190
BsmI GAATG_Cn'
Cuts at: 546 1386 4331 546
Size: 840 2945 4311
Fragments arranged by size:
4311 2945 840
BsmBI CGTCTCn'nnnn_
Cuts at: 1432 1432
Size: 8096
Bsp1286I G_dGCh'C
Cuts at: 133 560 778 1307 2703 3701 4121 4653 5110
Size: 427 218 529 1396 998 420 532 457
Cuts at: 5110 5721 5814 5888 6078 6140 6938 133
Size: 611 93 74 190 62 798 1291
Fragments arranged by size:
1396 1291 998 798 611 532 529 457
427 420 218 190 93 74 62
BspHI T'CATG_A
Cuts at: 639 1136 1374 1896 3488 4941 6528 639
Size: 497 238 522 1592 1453 1587 2207
Fragments arranged by size:
2207 1592 1587 1453 522 497 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1418 1501 2649 3194 5146 5360 5662 6043 1418
Size: 83 1148 545 1952 214 302 381 3471
Fragments arranged by size:
3471 1952 1148 545 381 302 214 83
BsrBI CCG'CTC
Cuts at: 50 6388 6442 7926 8091 50
Size: 6338 54 1484 165 55
Fragments arranged by size:
6338 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1029 6008 1029
Size: 4979 3117
BsrFI r'CCGG_y
Cuts at: 2751 3902 6094 6275 2751
Size: 1151 2192 181 4572
Fragments arranged by size:
4572 2192 1151 181
BsrGI T'GTAC_A
Cuts at: 199 199
Size: 8096
BssSI C'ACGA_G
Cuts at: 596 6367 7075 596
Size: 5771 708 1617
Fragments arranged by size:
5771 1617 708
BstAPI GCAn_nnn'nTGC
Cuts at: 5473 5473
Size: 8096
BstBI TT'CG_AA
Cuts at: 1617 4335 6457 1617
Size: 2718 2122 3256
Fragments arranged by size:
3256 2718 2122
BstXI CCAn_nnnn'nTGG
Cuts at: 3549 3549
Size: 8096
BstYI r'GATC_y
Cuts at: 1 296 937 1569 3387 3483 4015 4500 4590
Size: 295 641 632 1818 96 532 485 90
Cuts at: 4590 5048 5064 5121 5588 5611 5946 6192 6498
Size: 458 16 57 467 23 335 246 306
Cuts at: 6498 6510 6596 7648 1
Size: 12 86 1052 449
Fragments arranged by size:
1818 1052 641 632 532 485 467 458
449 335 306 295 246 96 90 86
57 23 16 12
Bsu36I CC'TnA_GG
Cuts at: 224 224
Size: 8096
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6046 6227 6390 6046
Size: 181 163 7752
Fragments arranged by size:
7752 181 163
BtsI GCAGTG_nn'
Cuts at: 721 1607 3579 5339 721
Size: 886 1972 1760 3478
Fragments arranged by size:
3478 1972 1760 886
ClaI AT'CG_AT
Cuts at: 3056 3056
Size: 8096
DraI TTT'AAA
Cuts at: 534 3446 5095 534
Size: 2912 1649 3535
Fragments arranged by size:
3535 2912 1649
DrdI GACnn_nn'nnGTC
Cuts at: 5802 7146 5802
Size: 1344 6752
EaeI y'GGCC_r
Cuts at: 2799 5681 5855 6246 6273 7618 2799
Size: 2882 174 391 27 1345 3277
Fragments arranged by size:
3277 2882 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5681 5681
Size: 8096
EarI CTCTTCn'nnn_
Cuts at: 563 1337 1904 2099 2546 2570 3023 4153 6119
Size: 774 567 195 447 24 453 1130 1966
Cuts at: 6119 6329 563
Size: 210 2330
Fragments arranged by size:
2330 1966 1130 774 567 453 447 210
195 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6304 7046 7192 7906 6304
Size: 742 146 714 6494
Fragments arranged by size:
6494 742 714 146
EcoICRI GAG'CTC
Cuts at: 131 558 776 1305 3699 4651 5108 131
Size: 427 218 529 2394 952 457 3119
Fragments arranged by size:
3119 2394 952 529 457 427 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 509 674 970 980 1142 1279 2570 3211
Size: 111 165 296 10 162 137 1291 641
Cuts at: 3211 3464 3569 3848 4005 4063 4118 4334 4436
Size: 253 105 279 157 58 55 216 102
Cuts at: 4436 4780 5921 6353 6706 7368 398
Size: 344 1141 432 353 662 1126
Fragments arranged by size:
1291 1141 1126 662 641 432 353 344
296 279 253 216 165 162 157 137
111 105 102 58 55 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4023 4023
Size: 8096
EcoO109I rG'GnC_Cy
Cuts at: 573 4546 5235 573
Size: 3973 689 3434
Fragments arranged by size:
3973 3434 689
EcoRI G'AATT_C
Cuts at: 635 2717 5100 635
Size: 2082 2383 3631
Fragments arranged by size:
3631 2383 2082
EcoRV GAT'ATC
Cuts at: 4311 4311
Size: 8096
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 592 3695 4381 4669 4810 6115 592
Size: 3103 686 288 141 1305 2573
Fragments arranged by size:
3103 2573 1305 686 288 141
FspI TGC'GCA
Cuts at: 5877 5877
Size: 8096
HaeII r_GCGC'y
Cuts at: 119 1651 5530 5778 7008 7779 119
Size: 1532 3879 248 1230 771 436
Fragments arranged by size:
3879 1532 1230 771 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 265 297 1224 1256 1960 1992 3022
Size: 32 118 32 927 32 704 32 1030
Cuts at: 3022 3054 4485 4517 4650 4682 4923 4955 5938
Size: 32 1431 32 133 32 241 32 983
Cuts at: 5938 5968 5970 6000 115
Size: 30 2 30 2211
Fragments arranged by size:
2211 1431 1030 983 927 704 241 133
118 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 420 3271 4532 5135 420
Size: 2851 1261 603 3381
Fragments arranged by size:
3381 2851 1261 603
HindIII A'AGCT_T
Cuts at: 3493 3493
Size: 8096
HpaI GTT'AAC
Cuts at: 420 4532 420
Size: 4112 3984
KasI G'GCGC_C
Cuts at: 115 5526 5774 115
Size: 5411 248 2437
Fragments arranged by size:
5411 2437 248
KpnI G_GTAC'C
Cuts at: 5116 5116
Size: 8096
MfeI C'AATT_G
Cuts at: 2332 2332
Size: 8096
MluI A'CGCG_T
Cuts at: 7529 7529
Size: 8096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 6855 7039 2915
Size: 3940 184 3972
Fragments arranged by size:
3972 3940 184
MscI TGG'CCA
Cuts at: 5857 5857
Size: 8096
MslI CAynn'nnrTG
Cuts at: 805 1379 1901 2869 3547 4080 4627 4697 6085
Size: 574 522 968 678 533 547 70 1388
Cuts at: 6085 6212 805
Size: 127 2689
Fragments arranged by size:
2689 1388 968 678 574 547 533 522
127 70
MspA1I CmG'CkG
Cuts at: 1661 2668 4996 5207 5521 5881 6665 6910 1661
Size: 1007 2328 211 314 360 784 245 2847
Fragments arranged by size:
2847 2328 1007 784 360 314 245 211
NaeI GCC'GGC
Cuts at: 2753 3904 6277 2753
Size: 1151 2373 4572
Fragments arranged by size:
4572 2373 1151
NarI GG'CG_CC
Cuts at: 116 5527 5775 116
Size: 5411 248 2437
Fragments arranged by size:
5411 2437 248
NdeI CA'TA_TG
Cuts at: 3323 3807 4963 3323
Size: 484 1156 6456
Fragments arranged by size:
6456 1156 484
NgoMIV G'CCGG_C
Cuts at: 2751 3902 6275 2751
Size: 1151 2373 4572
Fragments arranged by size:
4572 2373 1151
NsiI A_TGCA'T
Cuts at: 1452 2688 1452
Size: 1236 6860
NspI r_CATG'y
Cuts at: 790 1069 1148 1371 2296 5149 5649 7252 790
Size: 279 79 223 925 2853 500 1603 1634
Fragments arranged by size:
2853 1634 1603 925 500 279 223 79
PciI A'CATG_T
Cuts at: 1065 1367 7248 1065
Size: 302 5881 1913
Fragments arranged by size:
5881 1913 302
PflMI CCAn_nnn'nTGG
Cuts at: 2529 2529
Size: 8096
PfoI T'CCnGG_A
Cuts at: 739 739
Size: 8096
PmeI GTTT'AAAC
Cuts at: 5095 5095
Size: 8096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 765 797 3528 3560 6542 6574 765
Size: 32 2731 32 2982 32 2287
Fragments arranged by size:
2982 2731 2287 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 573 573
Size: 8096
PshAI GACnn'nnGTC
Cuts at: 2586 2586
Size: 8096
PstI C_TGCA'G
Cuts at: 2084 2267 3150 4879 4995 5143 5828 2084
Size: 183 883 1729 116 148 685 4352
Fragments arranged by size:
4352 1729 883 685 183 148 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8096
PvuII CAG'CTG
Cuts at: 2668 4996 5521 5881 2668
Size: 2328 525 360 4883
Fragments arranged by size:
4883 2328 525 360
RsrII CG'GwC_CG
Cuts at: 6291 6291
Size: 8096
SacI G_AGCT'C
Cuts at: 133 560 778 1307 3701 4653 5110 133
Size: 427 218 529 2394 952 457 3119
Fragments arranged by size:
3119 2394 952 529 457 427 218
SalI G'TCGA_C
Cuts at: 3269 5133 3269
Size: 1864 6232
SapI GCTCTTCn'nnn_
Cuts at: 2570 6119 6329 2570
Size: 3549 210 4337
Fragments arranged by size:
4337 3549 210
SbfI CC_TGCA'GG
Cuts at: 5143 5143
Size: 8096
ScaI AGT'ACT
Cuts at: 3260 3426 7613 3260
Size: 166 4187 3743
Fragments arranged by size:
4187 3743 166
SexAI A'CCwGG_T
Cuts at: 2159 2159
Size: 8096
SfcI C'TryA_G
Cuts at: 33 353 965 1074 1102 1390 2080 2263 3146
Size: 320 612 109 28 288 690 183 883
Cuts at: 3146 4520 4875 4991 5139 5824 6792 6983 33
Size: 1374 355 116 148 685 968 191 1146
Fragments arranged by size:
1374 1146 968 883 690 685 612 355
320 288 191 183 148 116 109 28
SfoI GGC'GCC
Cuts at: 117 5528 5776 117
Size: 5411 248 2437
Fragments arranged by size:
5411 2437 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8096
SmaI CCC'GGG
Cuts at: 5118 5118
Size: 8096
SmlI C'TyrA_G
Cuts at: 452 2845 2920 5251 6166 6880 7142 452
Size: 2393 75 2331 915 714 262 1406
Fragments arranged by size:
2393 2331 1406 915 714 262 75
SpeI A'CTAG_T
Cuts at: 67 3592 67
Size: 3525 4571
SphI G_CATG'C
Cuts at: 5149 5649 5149
Size: 500 7596
StuI AGG'CCT
Cuts at: 2740 2740
Size: 8096
StyI C'CwwG_G
Cuts at: 234 1037 3833 4542 4610 4835 5230 5584 234
Size: 803 2796 709 68 225 395 354 2746
Fragments arranged by size:
2796 2746 803 709 395 354 225 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 718 4687 4871 4993 5679 5812 6271 6480 718
Size: 3969 184 122 686 133 459 209 2334
Fragments arranged by size:
3969 2334 686 459 209 184 133 122
TatI w'GTAC_w
Cuts at: 199 1313 3258 3424 3595 7611 199
Size: 1114 1945 166 171 4016 684
Fragments arranged by size:
4016 1945 1114 684 171 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4003 4035 7613 7645 4003
Size: 32 3578 32 4454
Fragments arranged by size:
4454 3578 32 32
Tth111I GACn'n_nGTC
Cuts at: 2430 5893 2430
Size: 3463 4633
XbaI T'CTAG_A
Cuts at: 2434 3390 4747 5127 2434
Size: 956 1357 380 5403
Fragments arranged by size:
5403 1357 956 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2892 3546 5591 2892
Size: 654 2045 5397
Fragments arranged by size:
5397 2045 654
XhoI C'TCGA_G
Cuts at: 452 452
Size: 8096
XmaI C'CCGG_G
Cuts at: 5116 5116
Size: 8096
XmnI GAAnn'nnTTC
Cuts at: 2531 2531
Size: 8096
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI ApaI AscI BaeI BbvCI
BglI BmgBI BmtI BsiWI BspEI BssHII BstEII BstZ17I
BtgI CspCI DraIII FseI FspAI NcoI NheI NotI
NruI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI